CN220625913U - Automatic whole blood sample preparation instrument - Google Patents

Abstract

The utility model discloses an automatic whole blood sample preparation instrument which comprises a blood collection tube loading bin, a blood collection tube conveyor belt, a blood sample mixing device, a code scanner, a blood collection tube automatic centering device positioned at the tail end of the blood collection tube conveyor belt, a blood collection tube centering position and a screwing cover position, wherein the blood collection tube automatic centering device is positioned at the tail end of the blood collection tube conveyor belt; the blood collection tube transversely falls onto the blood collection tube conveyor belt from the blood collection tube loading bin; the blood sampling tube conveyor belt is matched with the blood sample mixing device to drive the blood sampling tube to rotate, mix and transmit, and the blood sampling tube is identified by the code scanning device; the evenly mixed blood collection tube falls into the blood collection tube alignment position in the upward posture of the tube cover under the action of gravity and an automatic blood collection tube alignment device; the sample tube is arranged at the same station or two independent stations, and a first manipulator device transfers the sample tube between the two stations; the cap screwing position is provided with an automatic cap opening and closing device for the blood sampling tube; the uncapped whole blood sample is sucked and distributed by a second manipulator device; the instrument automatically records the source of blood sample dispensed to each blood sample loading structure and the reagents dispensed.

Description

Automatic whole blood sample preparation instrument

Technical Field

The utility model relates to the field of medical equipment, in particular to an automatic whole blood sample preparation instrument.

Background

In medical research and clinical practice, whole blood is often required to be tested. The whole blood sample is often required to be distributed into a plurality of samples, and the blood sample is required to be fully mixed when being distributed as much as possible, so that the cells contained in each blood sample obtained by distribution are uniformly mixed, and the detection result obtained by detecting each blood sample obtained by distribution is consistent with the detection result obtained by detecting the original blood sample, namely the quality of the detection result is ensured.

Applicant found that prior art devices for dispensing only small amounts (no more than 50 ul) of whole blood sample per blood sample, but often require dispensing preparation of a larger amount of blood sample and dispensing of reagent simultaneously in medical laboratory work, while dispensing preparation of a larger amount (more than 100 ul) of whole blood sample is currently mainly dependent on manual work, and currently lack of equipment for automatic dispensing preparation of a larger amount of blood sample, simultaneous dispensing of reagent, high speed automation, which results in a complicated and inefficient part of the test operation.

Disclosure of Invention

The utility model aims to: the utility model aims to solve the technical problems of the prior art and provides an automatic whole blood sample preparation instrument which can be used for preparing and processing a large amount of whole blood samples.

In the present application, the term "multiple-unit dispensing of a larger amount of blood sample" refers to preparing a plurality of equal amounts of blood samples uniformly mixed according to the test requirements in a blood collection tube, and dispensing the blood samples into each of the loading vessels used for the test of the blood samples.

In order to solve the technical problems, the utility model discloses an automatic whole blood sample preparation instrument. The automatic whole blood sample preparation instrument comprises a blood collection tube loading bin for storing blood collection tubes to be processed, a blood collection tube conveying belt arranged below the blood collection tube loading bin, a blood sample mixing device matched with the blood collection tube conveying belt, a code scanner, a blood collection tube gravity automatic centering device positioned at the tail end of the blood collection tube conveying belt, a blood collection tube centering position, a screwing cover position, a first mechanical arm device, a second mechanical arm device and a loading structure for supporting a blood sample detection structure. The blood collection tube conveyor belt is in a strip shape with two sides symmetrically arranged, and a plurality of separation baffles are arranged on each blood collection tube conveyor belt at intervals. For each blood collection tube conveyor belt, the distance between two adjacent separation baffles only allows one blood collection tube to be placed. The blood collection tubes to be treated are orderly placed in the blood collection tube loading bin towards the same side according to the tube cover, and the blood collection tubes can transversely fall between two adjacent separation baffles on the blood collection tube conveyor belt one by one from the blood collection tube loading bin and move along the conveying direction under the drive of the blood collection tube conveyor belt and the separation baffles. The blood collection tube conveyor belt is matched with the blood sample mixing device to drive the blood collection tube to rotate, mix and transmit, and the code scanner is arranged above the blood collection tube conveyor belt and used for scanning and identifying bar codes on the rotating blood collection tube. The blood collection tube after the blood sample is rotated and evenly mixed automatically falls into the blood collection tube alignment position at the tail end of the blood collection tube conveyor belt after the tube cover is aligned downwards to the tail end of the blood collection tube under the combined action of gravity and the automatic blood collection tube gravity alignment device. The blood collection tube aligning position and the cap screwing position are the same station or two independent stations, and the blood collection tube is transferred between the two independent stations by the first manipulator device. The cap screwing position is provided with an automatic cap opening and closing device for the blood sampling tube. And the whole blood sample in the blood collection tube with the tube cover opened on the screwing position is sucked by a sample sucking tip loaded by the second manipulator device and is distributed to a blood sample detection structure on the loading structure. The preparation instrument automatically records the source of whole blood sample dispensed to each blood sample testing structure on the loading structure and the reagents dispensed.

Specifically, the second manipulator device comprises a sample extractor, and the sample sucking tip is loaded on the sample extractor. The sample sucking tip is a disposable liquid sucking tip which is communicated with a sample sucking passage inside the sample extractor. The preparation instrument further comprises a first storage area for storing unused disposable pipette tips, a first discarding bin for collecting the disposable pipette tips after use, and a first collecting bin for collecting the discarded blood collection tubes after distribution. The first manipulator device is also used for grabbing blood collection tubes which are distributed and covered by the tube cover from the cover screwing position to the first collection bin for discarding, and grabbing blood collection tubes with unqualified blood collection amounts in the blood collection tubes from the blood collection tube aligning position to the unqualified sample position.

Further, the preparation instrument further comprises more than 1 reagent dispensing means for dispensing a specific reagent into the sample testing structure for testing either before or after dispensing of the whole blood sample.

Specifically, the automatic gravity centering device for the blood collection tube is a transverse baffle plate which extends outwards from the tail end of one side, close to the cover end of the blood collection tube, of the blood collection tube conveyor belt. The blood collection tube positioning device is characterized in that a blood collection tube groove for receiving the blood collection tube is further formed in the blood collection tube positioning position, the blood collection tube groove comprises a cylindrical pipeline section and a horn mouth section connected to the upper portion of the cylindrical pipeline section, and the large diameter end of the horn mouth section faces upwards. The straightening refers to adjusting the posture of the blood collection tube into a vertical posture that the tube cover end faces upwards and the tail end of the tube faces downwards.

Specifically, when the heparin tube is put and is screwed up the position and be two independent stations, heparin tube is put and is provided with the heparin tube liquid level position judgement device that is used for detecting blood specimen height, heparin tube liquid level position judgement device includes light source and optical signal detector, the light source is used for shining the heparin tube that is located heparin tube and puts the position that is put, optical signal detector sets up in the same height of light source and corresponds in opposite directions for receive the light that is located heparin tube and put the light that the light source sent through the heparin tube, heparin tube liquid level position judgement device is through comparing above-mentioned light quantity and the threshold value of permeating of predetermineeing, thereby judge that is located heparin tube is put on the heparin tube and is put the blood specimen height in the heparin tube and be qualified.

When the blood collection tube is arranged at the same station in a straightening position and a screwing position, the sample suction passage is provided with an air pressure detection device, the air pressure detection device is used for measuring a negative pressure value formed when the disposable liquid suction tip stretches into the blood collection tube to a preset height for sample suction, and the preparation instrument judges whether the blood collection tube has a blood sample at the preset height by comparing the negative pressure value with a preset negative pressure threshold value.

In particular, the loading structure is a sample cup carrier or a test card loading tray. When the loading structure is a sample cup holder, the sample detection structure is a sample cup. When the loading structure is a test card loading tray, the sample detection structure is a test card. Each of the sample cup holders is used for loading an independent sample cup, or each of the sample cup holders is used for simultaneously loading a plurality of sample cups, and the sample cup holders are disc-shaped or strip-shaped.

Specifically, the preparation instrument is provided with an identification code for loading and dispensing the sample cup carrier or the test card loading tray, and the identification code records patient information of the whole blood sample currently loaded at each designated position of each loading structure and the dispensed reagent. The preparation instrument is also provided with an identification reading device matched with the identification code, and the identification code can be identified at the analyzer end according to the information of the original sample traced by the identification code.

Specifically, the blood sample mixing device comprises a mixing transmission belt, wherein the mixing transmission belt is positioned in the middle of the blood collection tube transmission belt, and the upper surface of the mixing transmission belt is higher than the upper surface of the blood collection tube transmission belt and lower than the top surface of the separation baffle. The mixing transmission belt and the blood collection tube transmission belt are transmitted in the same direction in a differential mode or in opposite directions. When the mixing conveyor belt continuously transmits, friction force between the blood collection tube on the mixing conveyor belt and the mixing conveyor belt drives the blood collection tube to synchronously move along with the mixing conveyor belt, and the blood collection tube is finally limited to form rotary motion at the position close to the separation baffle under the blocking of the separation baffle, so that the rotary mixing of the whole blood sample in the blood collection tube is realized.

Specifically, the blood sample mixing device is more than one group of extrusion type rotating devices, each group of extrusion type rotating devices comprises a passive rotating device and an active rotating device, and the passive rotating device and the active rotating device are respectively provided with two opposite sides of the blood collection tube conveying belt. When the blood collection tube conveyor belt pauses transmission, the passive rotating device and the active rotating device respectively prop against two ends of the blood collection tube at corresponding positions, and the active rotating device drives the blood collection tube to rotate, so that the whole blood sample in the blood collection tube is uniformly mixed in a rotating way. One group of extrusion type rotating devices drive the blood collection tube to rotate so as to realize the scanning and identification of the bar code on the rotating blood collection tube by the bar code scanner.

Specifically, the two sides of the blood collection tube conveyor belt are in a state that one side is higher than the other side, or the two sides are in a high-low staggered mode, and the whole blood sample is fully and uniformly mixed by rotating and flowing and reciprocating along the axial direction of the blood collection tube in the transmission process.

The beneficial effects are that:

(1) The automatic whole blood sample preparation instrument provided by the utility model is suitable for processing the whole blood sample with larger blood volume for detection, and each prepared blood sample is more than 100ul and is fully and uniformly mixed to achieve the uniformity very similar to or the same as the original blood sample, so that the content of various cells and liquid in each blood sample is ensured to be equal. The instrument has the functions of automatically transferring, uniformly mixing, bar code identification scanning, automatically opening and closing a cover, automatically distributing blood samples and automatically completing the addition of reagents into the distributed blood samples, and finally prepares the whole blood samples suitable for specific detection. The blood collection tubes to be processed are transversely dropped onto the blood collection tube conveyor belt one by one from the blood collection tube loading bin and are driven by the blood collection tube conveyor belt to move along the conveying direction. The blood sampling tube conveyor belt and the blood sample mixing device are matched to drive the blood sampling tube to rotate, and the code scanning device is used for scanning and identifying the bar code on the rotating blood sampling tube. The blood collection tube after the blood sample is rotated and evenly mixed is arranged in a posture that a tube cover is upward and the tube tail end is downward under the combined action of the gravity and the automatic blood collection tube gravity arranging device, and the blood collection tube falls into a blood collection tube arranging position. The sampling tube is transferred between two independent stations through a first manipulator device. The cap screwing position is provided with an automatic cap opening and closing device for the blood sampling tube. The whole blood sample in the screw-on position is dispensed by the second manipulator means to the blood sample testing structure on the loading structure. The preparation instrument automatically records the source of whole blood sample dispensed to each blood sample testing structure on the loading structure. Therefore, the preparation instrument realizes the functions of continuously and smoothly carrying out transmission mixing, automatic bar code reading and automatic distribution processing on a large number of whole blood samples. The preparation instrument has the characteristics of smooth sample processing flow, suitability for preparing more than 100ul of blood samples in a distributing way, and high sample processing speed, and can meet the requirements of various whole blood multi-item sample preparation and large-scale whole blood sample preparation, such as platelet function detection, thrombus elastography detection, blood viscosity detection, other blood cell component detection and other detection required whole blood sample preparation.

(2) The automatic whole blood sample preparation instrument provided by the embodiment of the utility model also has the functions of judging whether the height of the blood sample of the blood collection tube is qualified or not and automatically removing the blood collection tube with unqualified blood sample height from the blood collection tube to the waste blood collection tube collection bin.

(3) In an automatic whole blood sample preparation instrument provided by an embodiment of the utility model, the automatic gravity centering device of the blood collection tube is a transverse baffle plate extending outwards from the tail end of one side of the blood collection tube conveyor belt, which is close to the end of the blood collection tube cover. Under the action of the transverse baffle, the transverse blood collection tube deflects, the tube cover end deflects upwards due to the stop of the transverse baffle, the tube tail end deflects downwards, and finally falls into the cylindrical pipeline section in an upward vertical posture of the tail tube end under the guidance of the bell mouth section of the blood collection tube slot, so that the alignment of the blood collection tube is realized.

(4) In an automatic whole blood sample preparation instrument provided by one embodiment of the utility model, a plurality of separation baffles are arranged on a blood collection tube conveyor belt at intervals. Two adjacent separation baffles and the blood collection tube conveyor belt form a space for accommodating only one blood collection tube. The blood sample mixing device comprises a mixing driving belt, wherein the mixing driving belt is positioned in the middle of the blood collection tube driving belts arranged on two sides, and the upper surface of the mixing driving belt is higher than the upper surface of the blood collection tube driving belt but lower than the top surface of the separation baffle plate. The mixing transmission belt and the blood collection tube transmission belt are transmitted in the same direction in a differential mode or in opposite directions. When the mixing conveyor belt continuously transmits, the blood collection tube positioned on the mixing conveyor belt is driven by friction force between the blood collection tube and the mixing conveyor belt to synchronously move along with the mixing conveyor belt, and the blood collection tube finally rotates at a position close to the separation baffle under the blocking of the separation baffle, so that the rotation mixing of the whole blood sample in the blood collection tube is realized.

(5) The automatic whole blood sample preparation instrument provided by the utility model can be used as independent equipment, and can also be used as a special sample preparation unit for the front channel of the analyzer to form a whole with the instrument.

Drawings

The foregoing and/or other advantages of the utility model will become more apparent from the following detailed description of the utility model when taken in conjunction with the accompanying drawings and detailed description.

FIG. 1 is a top view of an automated whole blood sample preparation instrument according to a first embodiment of the present application;

FIG. 2 is a cross-sectional view taken along line A-A of a lancet tube loading bay and its lower lancet tube transfer related portion of the automated whole blood sample preparation instrument depicted in FIG. 1.

FIG. 3 is a cross-sectional view taken along line B-B of FIG. 1;

FIG. 4 is a top view of an automated whole blood sample preparation instrument according to a second embodiment of the present application;

fig. 5 is a schematic perspective view of a sample cup carrier loaded with sample cups in the form of multi-well cups in the automated whole blood sample preparation instrument shown in fig. 4.

Reference numerals of the present application are as follows:

the blood collection tube loading chamber 1, the blood collection tube 101, the blood collection tube conveyor 201, the blood sample mixing device 202, the code scanner 203, the separation baffle 204, the blood collection tube aligning position 4, the blood collection tube groove 401, the blood collection tube gravity automatic aligning device 5, the screwing cap position 6, the blood collection tube automatic cap opening and closing device 601, the detection card 701, the detection card loading disc 801, the sample cup bracket 802, the first storage area 9, the station 11 for loading reagent, the first collection chamber 12, the first discarding chamber 13, the reagent distributing arm 14, the first manipulator device 15, the second manipulator device 16, the light source 17, the light signal detector 18, the driving wheel 19, the driven wheel 20, the reject sample position 21, the first waiting area 22, the preparation area 23 and the second storage area 24.

Detailed Description

The technical solutions of the present application are described in detail below with reference to the accompanying drawings.

Example 1

A first embodiment of the present utility model provides an automated whole blood sample preparation instrument. As shown in fig. 1, the automatic whole blood sample preparation instrument comprises a blood collection tube loading bin 1 for storing a blood collection tube 101 to be processed, a blood collection tube conveyor belt 201 arranged below the blood collection tube loading bin 1, a blood sample mixing device 202 matched with the blood collection tube conveyor belt 201, a code scanner 203, a blood collection tube gravity automatic centering device 5 positioned at the tail end of the blood collection tube conveyor belt, a blood collection tube centering position 4, a screwing position 6, a first manipulator device 15, a second manipulator device 16 and a loading structure for supporting a blood sample detection structure. In this embodiment, as shown in fig. 1, the blood sample testing structure is a testing card 701, and the loading structure for holding the blood sample testing structure is a testing card loading tray 801.

As shown in fig. 1 and 2, the blood collection tube conveyor 201 is in a strip shape with two symmetrically arranged sides, and each blood collection tube conveyor 201 is provided with a plurality of separation baffles 204 arranged at intervals. For each lancet tube conveyor 201, the distance between two adjacent separation baffles 204 allows only one lancet tube 101 to be placed. That is, adjacent two separation baffles 204 form a space with the blood collection tube conveyor 201 for accommodating only one of the blood collection tubes.

As shown in fig. 1 and 2, the blood collection tubes 101 to be processed are orderly placed in the blood collection tube loading bin 1 according to the direction of the tube cover towards the same side, and the blood collection tubes transversely fall between two adjacent separation baffles 204 on the blood collection tube conveyor 201 one by one from the blood collection tube loading bin 1 and are driven by the blood collection tube conveyor 201 and the separation baffles 204 to move along the conveying direction. Meanwhile, the blood sample mixing device 202 drives each blood collection tube on the blood collection tube conveying belt 201 to rotate, mix and transfer, and the code scanner 203 is arranged above the blood collection tube conveying belt 201 and used for scanning and identifying bar codes on the rotating blood collection tubes 101. The blood collection tube 101 after the blood sample is rotationally and uniformly mixed is arranged at the tail end of the blood collection tube conveying belt 201 under the combined action of the gravity and the automatic blood collection tube gravity centering device 5, and after centering, a tube cover is formed to automatically fall into the blood collection tube centering position 4 at the tail end of the blood collection tube conveying belt 201 in a downward posture at the tail end of the tube. The blood collection tube aligning position 4 and the cap screwing position 6 are the same station or two independent stations, and as shown in fig. 1, the blood collection tube is transferred between the two independent stations by a first manipulator device 15. The cap screwing position 6 is provided with an automatic cap opening and closing device 601 for the blood collection tube. The whole blood sample in the blood collection tube with the tube cover opened on the cover screwing position 6 is sucked by the sample sucking tip loaded by the second manipulator device 16 and distributed to the blood sample detection structure on the loading structure. The preparation instrument automatically records the source of whole blood sample dispensed to each blood sample testing structure on the loading structure and the reagents dispensed.

The automatic transmission and mixing device can automatically realize functions of carrying out transmission and mixing on a large number of whole blood samples, distributing the uniformly mixed whole blood samples, automatically adding different reagents into each distributed blood sample, recording the original data information of each distributed blood sample and the like.

In this application, the centering means to adjust the posture of the blood collection tube 101 to a vertical posture in which the tube cap end faces upward and the tube tail end faces downward.

In particular, the second manipulator device 16 comprises a sample extractor on which the sample-absorbing tip is carried. The sample sucking tip is a disposable liquid sucking tip which is communicated with a sample sucking passage inside the sample extractor. Not shown in the figures. As shown in fig. 1, the preparation instrument further comprises a first storage area 9 for storing unused disposable pipette tips, a first disposal bin 13 for collecting disposable pipette tips after use, and a first collection bin 12 for collecting disposal blood collection tubes after dispensing. The first manipulator device 15 is further configured to grasp the blood collection tube 101 with the tube cover completely dispensed and capped from the cap screwing position 6 to the first collection bin 12 for discarding, and grasp the blood collection tube 101 with the failed blood collection amount in the blood collection tube from the blood collection tube aligning position 4 to the first collection bin 12 for discarding, or grasp the blood collection tube to a specially designated failed sample position 21.

Further, the preparation instrument further comprises more than 1 reagent dispensing means for dispensing a specific reagent to the sample detection structure for detection before or after dispensing of the whole blood sample. The preparation instrument comprising the reagent dispensing device may be either a whole blood sample preparation part of a fully automated instrument or a separate whole blood sample preparation instrument. Specifically, as shown in fig. 1, the reagent dispensing device comprises a station 11 for loading reagent, a reagent bottle arranged on the station 11 for loading reagent, and a reagent dispensing arm 14, and one or more reagent needles are arranged below the reagent dispensing arm 14 and connected with a diluter inside the instrument through a pipeline and a valve. When the reagent is dispensed, the reagent needle extends into the reagent in the reagent bottle, a diluter connected with the interior of the instrument sucks a certain amount of reagent in the reagent needle, then the reagent dispensing arm 14 drives the reagent needle to the position of the blood sample detection structure for loading the blood sample, and the diluter in the interior of the instrument pushes the reagent sucked in the reagent needle back into the blood sample detection structure for loading the blood sample.

Specifically, as shown in fig. 1 and 3, the automatic gravity centering device 5 for a blood collection tube is a lateral baffle extending outwards from the end of the blood collection tube conveyor 201 near the end of the blood collection tube. The blood collection tube positioning position 4 is also provided with a blood collection tube groove 401 for receiving a blood collection tube. The blood collection tube slot 401 comprises a cylindrical pipeline section and a flare section connected to the upper side of the cylindrical pipeline section, and the large diameter end of the flare section faces upwards.

In order to remove blood collection tubes with unqualified height blood samples, the preparation instrument has the function of detecting whether the blood collection tube is qualified enough in blood sample loading height. In a specific embodiment, as shown in fig. 1, the blood collection tube positioning position 4 and the cap screwing position 6 are two independent stations, and the blood collection tube positioning position 4 is provided with a blood collection tube liquid level position judging device for detecting the height of a blood sample. As shown in fig. 2, the device for determining the liquid level of the blood collection tube comprises a light source 17 and a light signal detector 18, which are positioned at the same height and at opposite positions, wherein the light source 17 is used for irradiating the blood collection tube positioned at the blood collection tube positioning position 4, and the light signal detector 18 is used for receiving the transmitted light quantity of the light emitted by the light source 17 positioned at the blood collection tube positioning position 4 through the blood collection tube. The blood collection tube liquid level judging device is arranged at a certain height position outside the blood collection tube in the blood collection tube setting position 4, and judges whether the height of the blood sample in the blood collection tube positioned in the blood collection tube setting position 4 is qualified or not by comparing the transmitted light quantity with a preset threshold value, so that whether the blood sample loading quantity in each blood collection tube is enough or not is evaluated. Specifically, when the blood collection tube falls into the blood collection tube setting position 4, the instrument automatically starts the light source 17 and the optical signal detector 18 corresponding to the position to work. The light source 17 is a long wavelength light source of 600nm or more, and the positions of the light source 17 and the optical signal detector 18 are located at a height where the blood sampling tube is required to be reached. When the blood sample loading reaches the height, the light emitted by the light source 17 cannot pass through the blood sample in the blood sampling tube, so that the light signal detector 18 cannot detect the signal from the light source 17, or the signal is very weak, and the measurable absorbance value is more than 2.0OD; however, when the blood sample in the blood collection tube is insufficient, the blood sample cannot reach the height, and at this time, the light emitted by the light source 17 is not blocked by the blood sample in the region of the blood collection tube, and even if the wall of the tube is provided with a blood wall-mounted film, the light emitted by the light source can transmit a stronger light signal, so that the light signal detector 18 can detect the stronger signal from the light source 17, and the measured absorbance value is less than 1.0OD. In another specific embodiment, the blood collection tube positioning position 4 and the screwing position 6 are the same station, the sample suction passage is provided with an air pressure detection device, the air pressure detection device is used for measuring a negative pressure value formed when the disposable pipette tip stretches into the blood collection tube to a preset height for sample suction, and the preparation instrument judges whether the blood collection tube has a blood sample at the preset height by comparing the negative pressure value with a preset negative pressure threshold value. Specifically, when the blood sample is sucked, the second manipulator device 16 drives the disposable pipette tip to descend into the blood collection tube to a certain depth, and the sample extractor sucks the blood sample. When no blood sample exists at the position, the air pressure detection device arranged on the sample sucking passage cannot detect pressure change, so that the blood collecting tube is high in the position without blood sample. However, when a blood sample is present at the height of the blood collection tube, the air pressure detecting device mounted on the suction passage can detect a decrease in pressure in the tube when the sample is sucked by the sample extractor, indicating that the blood collection tube is high enough to collect a blood sample at the height of the blood collection tube.

Specifically, the preparation instrument is provided with identification codes for the loading structures after loading and dispensing, and the identification codes record patient information of whole blood samples currently loaded at each designated position of each loading structure and dispensed reagents. The preparation instrument is also provided with an identification reading device matched with the identification code, and the identification code can be identified at the analyzer end according to the information of the original sample traced by the identification code.

The system can drive and mix the blood collection tube uniformly. In a specific embodiment, as shown in fig. 1-3, the blood sample mixing device 202 includes a mixing belt positioned in the middle of the blood collection tube conveyor 201, and as shown in fig. 3, with its upper surface above the upper surface of the blood collection tube conveyor 201 and below the top surface of the separation barrier 204. The mixing transmission belt and the blood collection tube transmission belt 201 are transmitted in the same direction and in different directions or opposite directions. When the mixing conveyor belt continuously transmits, friction force between the blood collection tube on the mixing conveyor belt and the mixing conveyor belt drives the blood collection tube to synchronously move along with the mixing conveyor belt, the blood collection tube cannot do linear movement under the blocking of the separation baffle 204, but is limited to form rotary movement at the position close to the separation baffle 204, and therefore rotary mixing of whole blood samples in the blood collection tube is achieved. In another particular embodiment, the blood sample mixing device 202 is a set of more than one squeeze-type rotating devices, not shown. Each group of extrusion type rotating devices comprises a passive rotating device and an active rotating device, and the passive rotating device and the active rotating device are respectively provided with two opposite sides of the blood collection tube conveyor belt 201. When the blood collection tube conveyor belt 201 pauses transmission, the passive rotating device and the active rotating device respectively prop against two ends of the blood collection tube at corresponding positions, and the active rotating device drives the blood collection tube to rotate, so that the whole blood sample in the blood collection tube is uniformly mixed in a rotating way. At least one group of extrusion type rotating devices is arranged at the code scanner 203, and the extrusion type rotating devices drive the blood collection tube to rotate, so that the code scanner 203 scans and identifies the bar code on the rotating blood collection tube 101.

Example 2

In a second embodiment of the present application, the loading structure is a sample cup carrier 802 and the sample detection structure is a sample cup 702. The sample cup holder 802 may be a sample cup holder for loading an independent sample cup 702, or the sample cup holder 802 may be a sample cup holder capable of simultaneously loading a plurality of sample cups 702, which is disk-shaped or bar-shaped. For the same sample cup holder 802, the plurality of sample cups 702 may be a plurality of independent single-cup sample cups or may be connected together to form a multi-well cup. In one particular embodiment, as shown in fig. 4 and 5, the sample cup holder 802 is used to simultaneously load a plurality of sample cups 702, and the plurality of sample cups 702 are connected together to form a multi-well cup.

As shown in fig. 4, the sample cup carrier 802 loaded with the unused sample cups 702 is placed in the first waiting area 22 waiting for loading of the blood sample, and the sample cup carrier 802 loaded with the unused sample cups 702 is gradually transferred to the preparation area 23. Within the preparation area 23, the whole blood sample in the blood collection tube after opening the tube cap at the cap screwing station 6 is dispensed by the second manipulator device 16 into the sample cup 702 of the corresponding sample cup holder 802. Reagents are added by the reagent dispensing arm 14 to each of the sample cups 702 in which the blood sample has been dispensed. The designated reagent is mixed with the blood sample to obtain the blood sample for testing. The sample cup carrier 802, loaded with sample cups 702 after sample processing is then transferred to the second storage area 24 for storage. The double-headed arrow in fig. 4 indicates the direction of movement of the reagent dispensing arm 14 employed in the present embodiment. The transfer of the sample cup holder 802 is prior art and is therefore not described in detail in this application.

In any of the above embodiments, the two sides of the blood collection tube conveyor 201 are higher than each other, or the two sides are staggered. When the two sides of the blood collection tube conveyor 201 are staggered in height, the whole blood sample flows in a rotating way and flows back and forth along the axial direction of the blood collection tube in the transmission process, so that the blood sample is more fully and uniformly mixed.

In any of the above embodiments, as shown in fig. 2, the preparing apparatus includes a driving pulley 19 and a driven pulley 20, and the blood collection tube conveyor 201 is rotatably connected to the outer circumferential sides of the driving pulley 19 and the driven pulley 20. In a specific embodiment, the driving wheel is driven to rotate by a stepping motor, and then the driven wheel is matched with the driving wheel to drive the blood collection tube conveying belt 201 to move.

The application also provides a working method of the automatic whole blood sample preparation instrument, which is implemented by adopting the automatic whole blood sample preparation instrument, and comprises the following steps:

step 1, horizontally placing all blood collection tubes to be treated into a blood collection tube loading bin 1, and flatly placing the blood collection tubes into the blood collection tube loading bin 1 in a mode that tube cover ends are unified at the same end and tube tail ends are at the other end during placing; the blood collection tubes in the blood collection tube loading bin 1 fall onto the blood collection tube conveyor belt 201 under the action of gravity, and the blood collection tubes are separated by a separation baffle 204 arranged on the conveyor belt; the blood collection tube moves unidirectionally along the conveying direction along with the blood collection tube conveying belt 201; in the process of unidirectional movement of the blood collection tube along the conveying direction, the blood collection tube is driven by the blood collection tube mixing device 202, the separation baffle 204 on the blood collection tube conveying belt 201 stops the blood collection tube from rotating to mix the blood collection tube, and the code scanner 203 performs identification record on the bar code on the wall of the blood collection tube in the process of the rotation movement of the blood collection tube;

step 2, after the blood collection tube at the tail end of the blood collection tube conveying belt 201 leaves the blood collection tube conveying belt 201, switching from a transverse state to a vertical state with the tube cover end upward and the tube tail end downward to fall under the combined action of gravity and the automatic blood collection tube gravity centering device 5, and enabling the blood collection tube centering position 4 to receive the blood collection tube falling in the vertical state;

step 3, arranging a blood collection tube liquid level position judging device at the blood collection tube setting position 4, wherein the blood collection tube liquid level position judging device is used for detecting whether the blood sample amount in the blood collection tube is enough or not to detect the required blood sample amount, if not, prompting the blood collection tube to be not treated as a qualified blood collection tube, and if so, continuing to treat the blood collection tube according to the normal process;

step 4: the first manipulator device 15 grabs the blood collection tube from the blood collection tube setting position 4, and if the blood collection tube meets the requirements, the first manipulator device 15 grabs the blood collection tube to the cap screwing position 6; however, if the label bar code can not read or the liquid level detection is insufficient, the first manipulator device 15 sends the blood sample to the unqualified sample position 21 to be processed;

step 5: the blood collection tube is vertically placed at the cap screwing position 6, the tube cap end is upward, the cap screwing position 6 is provided with a blood collection tube automatic cap opening and closing device 601, the blood collection tube is opened by the blood collection tube automatic cap opening and closing device 601, and the cap is removed, so that the upper end of the blood collection tube is completely unobstructed;

step 6: the second manipulator device 16 drives the disposable pipette tip to extend into the blood collection tube of the screwing cap position 6 for sucking; after the disposable pipette tip draws a whole blood sample, the second manipulator device 16 drives the disposable pipette tip into a sample detection structure and distributes the drawn whole blood sample into the structure; repeating the steps to distribute the whole blood sample into more than 1 sample test structures according to test requirements;

step 7: the instrument records or marks the loading structure loading the sample detection structure; the prepared sample can be used for detection; alternatively, after the second manipulator device 16 dispenses the blood sample, the reagent dispensing device of the apparatus also adds reagents to each of the above-mentioned sample detection structures of the dispensed blood sample, mixes the reagents with the blood sample, and completes preparation of the blood sample for detection;

step 8: in the screwing position 6, the blood collection tube with the distributed blood collection tube is covered by the automatic cover opening and closing device 601, the distributed blood collection tube with the covered tube cover is grabbed to the first collection bin 12 to be discarded by the first manipulator device 15, the disposable pipette tip after being used is discarded to the first discarding bin 13 by the second manipulator device 16, and then a new disposable pipette tip is loaded on the second manipulator device 16 for standby; the blood collection tube conveyor 201 transfers forward and sends a new blood collection tube to the blood collection tube alignment position 4, and the instrument again performs the same treatment on the new blood collection tube according to the above flow until all the blood collection tubes to be treated in the blood collection tube loading bin 1 are treated.

The utility model provides an idea and a method for an automatic whole blood sample preparation instrument, and the method and the way for realizing the technical scheme are numerous, the above description is only a preferred embodiment of the utility model, and it should be noted that, for a person skilled in the art, several improvements and modifications can be made without departing from the principle of the utility model, and the improvements and modifications should be regarded as the protection scope of the utility model. The components not explicitly described in this embodiment can be implemented by using the prior art.

Claims (10)

1. An automatic whole blood sample preparation instrument is characterized by comprising a blood collection tube loading bin (1) for storing blood collection tubes to be processed, a blood collection tube conveying belt (201) arranged below the blood collection tube loading bin (1), a blood collection tube mixing device (202) matched with the blood collection tube conveying belt (201), a code scanner (203), a blood collection tube gravity automatic centering device (5) positioned at the tail end of the blood collection tube conveying belt, a blood collection tube centering position (4), a screwing position (6), a first mechanical arm device (15), a second mechanical arm device (16) and a loading structure for supporting a blood collection detection structure; the blood collection tube conveyor belts (201) are in a strip shape with two sides symmetrically arranged, and a plurality of separation baffles (204) are arranged on each blood collection tube conveyor belt (201) at intervals; for each blood collection tube conveyor belt (201), the distance between two adjacent separation baffles (204) only allows one blood collection tube (101) to be placed; the blood collection tubes (101) to be treated are orderly placed in the blood collection tube loading bin (1) towards the same side according to the tube cover, the blood collection tubes can transversely fall between two adjacent separation baffles (204) on the blood collection tube conveyor belt (201) one by one from the blood collection tube loading bin (1) and move along the conveying direction under the driving of the blood collection tube conveyor belt (201) and the separation baffles (204); the blood collection tube conveyor belt (201) is matched with the blood sample mixing device (202) to drive the blood collection tube to rotate, mix and transmit, and the code scanner (203) is arranged above the blood collection tube conveyor belt (201) and used for scanning and identifying bar codes on the rotating blood collection tube; the blood collection tube after the blood sample is rotated and evenly mixed automatically falls into the blood collection tube righting position (4) at the tail end of the blood collection tube conveyor belt (201) under the combined action of gravity and the automatic blood collection tube gravity righting device (5) after the tube cover is righted downwards towards the tail end of the blood collection tube; the blood collection tube aligning position (4) and the cap screwing position (6) are the same station or two independent stations, and the blood collection tube is transferred between the two independent stations by a first manipulator device (15); the cap screwing position (6) is provided with an automatic cap opening and closing device (601) for the blood collection tube; the whole blood sample in the blood collection tube with the tube cover opened on the screwing position (6) is sucked by a sample sucking tip loaded by a second manipulator device (16) and is distributed to a blood sample detection structure on the loading structure; the preparation instrument automatically records the source of whole blood sample dispensed to each blood sample testing structure on the loading structure and the reagents dispensed.

2. An automated whole blood sample preparation instrument according to claim 1, wherein the second manipulator device (16) comprises a sample extractor on which the sample-absorbing tip is carried; the sample sucking tip is a disposable liquid sucking tip which is communicated with a sample sucking passage in the sample extractor; the preparation instrument also comprises a first storage area (9) for storing unused disposable pipette tips, a first discarding bin (13) for collecting the disposable pipette tips after use and a first collecting bin (12) for collecting the discarded blood collection tubes after distribution; the first manipulator device (15) is also used for grabbing blood collection tubes which are distributed and covered by the tube cover from the cover screwing position (6) to the first collecting bin (12) to be abandoned, and grabbing blood collection tubes with unqualified blood collection amounts in the blood collection tubes from the blood collection tube arranging position (4) to the unqualified sample position (21).

3. An automated whole blood sample preparation instrument according to claim 2, further comprising more than 1 reagent dispensing means for dispensing specific reagents into the sample testing structure for testing either before or after the whole blood sample is dispensed.

4. An automated whole blood sample preparation device according to any one of claims 1 to 3, wherein the automatic lancet gravity centering device (5) is a transverse baffle extending outwardly from the end of the lancet conveyor belt (201) on the side of the lancet tube cap end; the blood collection tube positioning device is characterized in that the blood collection tube positioning device (4) is further provided with a blood collection tube groove (401) for receiving a blood collection tube, the blood collection tube groove (401) comprises a cylindrical pipeline section and a horn mouth section connected to the upper part of the cylindrical pipeline section, and the large diameter end of the horn mouth section faces upwards; the straightening refers to adjusting the posture of the blood collection tube into a vertical posture that the tube cover end faces upwards and the tail end of the tube faces downwards.

5. An automated whole blood sample preparation instrument according to any one of claims 2 to 3, wherein when the blood collection tube alignment position (4) and the screwing position (6) are two independent stations, the blood collection tube alignment position (4) is provided with blood collection tube liquid level position judgment means for detecting the height of a blood sample, the blood collection tube liquid level position judgment means comprises a light source (17) and an optical signal detector (18), the light source (17) is used for irradiating the blood collection tube positioned at the blood collection tube alignment position (4), the optical signal detector (18) is arranged at the position which corresponds to the position of the light source (17) at the same height and opposite to the light source, and is used for receiving the transmitted light quantity of the light emitted by the light source (17) positioned at the blood collection tube alignment position (4) through the blood collection tube, and the blood collection tube liquid level position judgment means judges whether the height of the blood sample positioned in the blood collection tube positioned at the blood collection tube alignment position (4) is qualified or not by comparing the transmitted light quantity with a preset threshold;

when the blood collection tube is arranged at the same station in the straightening position (4) and the screwing position (6), an air pressure detection device is arranged on the sample suction passage and used for measuring a negative pressure value formed when the disposable liquid suction tip stretches into the blood collection tube to a preset height for sample suction, and the preparation instrument judges whether the blood collection tube has a blood sample at the preset height by comparing the negative pressure value with a preset negative pressure threshold value.

6. An automated whole blood sample preparation instrument according to claim 3, wherein the loading structure is a sample cup carrier (802) or a test card loading tray (801); when the loading structure is a sample cup carrier (802), the sample detection structure is a sample cup (702); when the loading structure is a test card loading tray (801), the sample detection structure is a test card (701); each of the sample cup holders (802) is used for loading a separate sample cup (702), or each of the sample cup holders (802) is used for simultaneously loading a plurality of sample cups (702), and the sample cup holders (802) are disc-shaped or strip-shaped.

7. An automated whole blood sample preparation instrument according to claim 6, wherein the preparation instrument is provided with identification codes for loading the dispensed sample cup holders (802) or the test card loading trays (801), the identification codes describing patient information of the whole blood sample currently loaded at each designated location on each loading structure and the dispensed reagents; the preparation instrument is also provided with an identification reading device matched with the identification code, and the identification code can be identified at the analyzer end according to the information of the original sample traced by the identification code.

8. An automated whole blood sample preparation instrument according to any one of claims 1 to 3, wherein the blood sample mixing device (202) comprises a mixing belt located in the middle of the blood collection tube conveyor (201) and having an upper surface above the upper surface of the blood collection tube conveyor (201) below the top surface of the separation barrier (204); the mixing transmission belt and the blood collection tube transmission belt (201) are transmitted in the same direction and in different directions; when the mixing conveyor belt continuously transmits, friction force between the blood collection tube on the mixing conveyor belt and the mixing conveyor belt drives the blood collection tube to synchronously move along with the mixing conveyor belt, and the blood collection tube is finally limited to form rotary motion at the position close to the separation baffle (204) under the blocking of the separation baffle (204), so that rotary mixing of whole blood samples in the blood collection tube is realized.

9. An automated whole blood sample preparation instrument according to any one of claims 1 to 3, wherein the blood sample mixing device (202) is a plurality of sets of squeeze-type rotation devices, each set of squeeze-type rotation devices comprising a passive rotation device and an active rotation device, the passive rotation device and the active rotation device being provided with opposite sides of the blood collection tube conveyor belt (201), respectively; when the blood collection tube conveyor belt (201) pauses transmission, the passive rotating device and the active rotating device respectively prop against two ends of the blood collection tube at corresponding positions, and the active rotating device drives the blood collection tube to rotate so as to realize rotation and uniform mixing of whole blood samples in the blood collection tube; wherein the method comprises the steps of

The extrusion type rotating device drives the blood collection tube to rotate so as to realize the scanning and identification of the bar code on the rotating blood collection tube (101) by the code scanner (203).

10. An automated whole blood sample preparation device according to any one of claims 1 to 3, wherein the sides of the lance conveyor belt (201) are higher than each other or are staggered in height, and the whole blood sample is thoroughly mixed during transfer by both rotating and reciprocating along the lance axis.