CN220587365U

CN220587365U - Low-temperature storage device for immune cells

Info

Publication number: CN220587365U
Application number: CN202322303339.6U
Authority: CN
Inventors: 徐晓琳; 刘明明
Original assignee: Shandong Zhangjiang Bioengineering Co ltd
Current assignee: Shandong Zhangjiang Bioengineering Co ltd
Priority date: 2023-08-24
Filing date: 2023-08-24
Publication date: 2024-03-15
Anticipated expiration: 2033-08-24

Abstract

The utility model relates to the technical field of immune cells, in particular to a low-temperature storage device for immune cells, which comprises a box body, a hydraulic pump, a first motor, an installation box and a taking bin; the hydraulic pump is installed at the box top, and first motor is installed at the box top, and the threaded rod is connected to first motor, and the threaded rod is rotated and is installed in the box, threaded rod threaded connection slide, slide intermediate bearing connection movable rod, movable rod bottom connection second motor, second motor are installed in the mounting box, and the mounting bracket is connected to the slide, and mounting bracket bottom and mounting box are connected, and box one side lateral wall is connected in visual window position department and is taken. The utility model uses the combination of the first motor, the threaded rod and the sliding plate to enable the cell bottle to automatically lift along with the sliding plate, and the cell bottle can be taken by the taking bin; through the cooperation of second motor and movable rod, make the cell bottle rotate, cell bottle and liquid nitrogen more comprehensive contact freeze the deposit, also can rotate the cell bottle to the storehouse horizontal position of taking simultaneously, conveniently take.

Description

Low-temperature storage device for immune cells

Technical Field

The utility model relates to the technical field of immune cells, in particular to a low-temperature storage device for immune cells.

Background

Immune cells refer to cells involved in or associated with an immune response. Including lymphocytes, dendritic cells, monocytes/macrophages, granulocytes, mast cells, and the like. Immune cells can be divided into a variety of classes, and various immune cells play an important role in humans. Immune cells are commonly called white blood cells, and include congenital lymphocytes, various phagocytes and the like, lymphocytes capable of recognizing antigens and generating specific immune responses, and the like, and the immune cells need to be stored at a low temperature in storage so as to ensure that the immune cells can be stored for a long time and can be effectively used after subsequent use.

Chinese authorized utility model disclosure (bulletin) number: the utility model discloses a low temperature storage device for immune cells in CN215809548U, this low temperature storage device for immune cells includes box, liquid nitrogen refrigeration subassembly, automatic lifting unit, stop gear and automatic illuminating mechanism, although can the automatic lifting test tube, conveniently take the test tube, when opening the container, liquid nitrogen gasifies in the twinkling of an eye and frostbite operating personnel easily, has certain limitation, simultaneously, when operating personnel take out the cell, makes cell preservation device produce the bacterium easily, and can not carry out classified storage to the cell type, is inconvenient for seek and takes out. In view of this drawback, we have devised a cryogenic storage device for immune cells.

Disclosure of Invention

The present utility model is directed to a low temperature storage device for immune cells, which solves the above-mentioned problems of the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a low temperature storage device for immunocyte, includes box, hydraulic pump, first motor, install bin, take the storehouse, the cavity has been seted up to the box inner wall, box top fixed mounting has the hydraulic pump, install the transfer line on the infusion port of hydraulic pump, the one end that the hydraulic pump was kept away from to the transfer line extends to in the cavity, fixed mounting has liquid level sensor on one side inner wall of cavity, box top fixed mounting has first motor, first motor output is connected with the threaded rod, the one end that the threaded rod kept away from first motor rotates and installs in the box, threaded rod threaded connection has the slide, threaded rod's one end that the slide kept away from the threaded rod alternates respectively and is connected with two dead levers around, dead lever top and bottom respectively with box inner chamber top and bottom fixed connection, slide intermediate bearing is connected with the movable rod, two turntables of respectively fixedly connected with in movable rod top and below, the equidistance lateral wall fixed mounting has a plurality of electric telescopic handle, the sleeve end is provided with the test tube inner chamber, the test tube cover is placed to place the first motor, the first motor output shaft is connected with the first motor, the other end fixed connection has the bulb, the bulb is connected with the bulb is fixed with the bottom the first motor, the inside tube is connected with the first end, the bulb is fixed with the bottom of the bulb is fixed connection, the bulb is connected with the bottom cylinder top fixed with the first end, the bulb is connected with the bottom of the bulb fixed mounting bracket, the bulb is fixed connection. The visual window is arranged on the side wall of one side of the box body, the taking bin is connected to the side wall of one side of the box body at the position of the visual window, the top of the taking bin is connected with a taking bin cover plate, and the joint of the box body and the taking bin is connected with a rotary cover plate.

Preferably, a temperature and humidity sensor and a heat conducting fin are respectively and fixedly arranged on the side wall of the inner cavity of the box body.

Preferably, the top of the taking bin is hinged with a cover plate of the taking bin, and the joint of the box body and the taking bin is hinged with a rotary cover plate.

Preferably, the telescopic link tip is equipped with splint, splint one side and cell bottle's lateral wall laminating mutually, splint are with cell bottle lateral wall assorted circular-arc.

Preferably, the side wall of the infusion tube is provided with a control valve.

Preferably, the visual window is communicated with the taking bin.

Compared with the prior art, the utility model has the beneficial effects that: the utility model utilizes the combination of the first motor, the threaded rod and the sliding plate to enable the cell bottle to automatically lift along with the sliding plate, so that the cell bottle can be moved to the horizontal position of the taking bin, and the cell bottle can be taken by the taking bin, thereby avoiding frostbite to operators caused by instantaneous gasification of liquid nitrogen when the cell bottle is taken by opening the container, and simultaneously avoiding bacteria in the container and damaging immune cells stored in the container when the cell bottle is taken out by the operator; different cell bottles are respectively placed at the inner cavity of the test tube sleeve, so that test tubes of different cells can be conveniently sorted and stored, subsequent cell searching is convenient, and the cell bottles are taken out from the taking bin, so that the problem that the existing human immune cell storage device cannot be used for sorting and storing cell types and is inconvenient to search and take out is solved; through the cooperation between the second motor and the movable rod, the cell bottle can slowly move, the cell bottle and the liquid nitrogen are more comprehensively contacted and frozen, the cell storage quality in the cell bottle is ensured, the existing human immune cell storage device is solved, and meanwhile, the cell bottle can be rotated to the horizontal position of the taking bin, so that the cell bottle is convenient to take; the box body can be illuminated through the cooperation of the third motor and the air cylinder, so that the cell bottle can be conveniently taken out; through telescopic link and spring cooperation use, can press from both sides the cell bottle tightly, and the telescopic link can the not cell bottle of equidimension of centre gripping, makes the scope of storage cell bottle bigger.

Drawings

FIG. 1 is a cross-sectional view of the present utility model;

FIG. 2 is an enlarged view of FIG. 1A;

FIG. 3 is an enlarged view of FIG. 1B;

FIG. 4 is a schematic view of a cavity structure of the present utility model;

FIG. 5 is a top view of the present utility model;

FIG. 6 is a schematic view of the structure of the slide plate, movable rod and cell flask of the present utility model;

FIG. 7 is a schematic view showing the internal structure of the test tube sleeve according to the present utility model;

fig. 8 is a schematic view of the internal structure of the picking chamber of the present utility model.

In the figure: 1. a case; 2. a hydraulic pump; 3. a first motor; 4. a mounting box; 5. taking a bin; 6. a cavity; 7. an infusion tube; 8. a liquid level sensor; 9. a threaded rod; 10. a slide plate; 11. a fixed rod; 12. a movable rod; 13. a turntable; 14. an electric telescopic rod; 15. a test tube sleeve; 16. a cell bottle; 17. a telescopic rod; 18. a spring; 19. a second motor; 20. a third motor; 21. a fixing plate; 22. a bulb; 23. a cylinder; 24. a visual window; 25. taking a bin cover plate; 26. rotating the cover plate; 27. a temperature and humidity sensor; 28. a heat conductive sheet; 29. a clamping plate; 30. a control valve; 31. and (5) mounting a frame.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In the description of the present utility model, it should be noted that the azimuth or positional relationship indicated by the terms "vertical", "upper", "lower", "horizontal", etc. are based on the azimuth or positional relationship shown in the drawings, and are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or element referred to must have a specific azimuth, be constructed and operated in a specific azimuth, and thus should not be construed as limiting the present utility model.

In the description of the present utility model, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

Referring to fig. 1-8, the present utility model provides a technical solution: the utility model provides a low temperature storage device for immune cells, which comprises a box body 1, a hydraulic pump 2, a first motor 3, the mounting box 4, the storehouse 5 of taking, cavity 6 has been seted up to the box body 1 inner wall, the hydraulic pump 2 is fixedly installed at box body 1 top, install transfer line 7 on the infusion port of hydraulic pump 2, the one end that transfer line 7 kept away from hydraulic pump 2 extends to in cavity 6, fixedly installed liquid level sensor 8 on the inner wall of one side of cavity 6, the fixed mounting in box body 1 top one end has a first motor 3, first motor 3 output is connected with threaded rod 9, threaded rod 9 keeps away from the one end rotation of first motor 3 and installs in box body 1, threaded rod 9 threaded connection has slide 10, slide 10 keep away from threaded rod 9 one end front and back respectively alternate and be connected with two dead levers 11, dead lever 11 top and bottom respectively with box body 1 inner chamber top and bottom fixed connection, the middle bearing of the slide plate 10 is connected with a movable rod 12, two turntables 13 are respectively and fixedly connected above and below the movable rod 12, a plurality of electric telescopic rods 14 are fixedly installed on the side walls of the turntables 13 at equal intervals, a test tube sleeve 15 is arranged at the end part of each electric telescopic rod 14, a cell bottle 16 is placed in the inner cavity of each test tube sleeve 15, a plurality of telescopic rods 17 are connected between the inner side walls of the test tube sleeves 15 and the outer side walls of the cell bottles 16, springs 18 are sleeved outside the telescopic rods 17, the bottom ends of the movable rods 12 are connected with second motors 19, the second motors 19 are fixedly installed in the installation boxes 4, an installation frame 31 is fixedly connected with the bottom of the installation frame 31 and the installation boxes 4, a third motor 20 is installed on the inner wall of the top of the box 1, a fixed plate 21 is connected with an output shaft of the third motor 20, a bulb 22 is hinged at the bottom of the fixed plate 21, one end of a cylinder 23 is hinged at the bottom of one side of the fixed plate 21, the other end of the air cylinder 23 is hinged with the bulb 22, a visual window 24 is formed in the side wall of one side of the box body 1, the taking bin 5 is connected to the side wall of one side of the box body 1 at the position of the visual window 24, a taking bin cover plate 25 is connected to the top of the taking bin 5, and a rotary cover plate 26 is connected to the joint of the box body 1 and the taking bin 5.

Further, the temperature and humidity sensor 27 and the heat conducting fin 28 are fixedly installed on the side wall of the inner cavity of the box body 1 respectively, so that the temperature in the box body 1 can be conveniently monitored.

Further, the top of the taking bin 5 is hinged with a taking bin cover plate 25, and the joint of the box body 1 and the taking bin 5 is hinged with a rotary cover plate 26.

Further, the end of the telescopic rod 17 is provided with a clamping plate 29, one side of the clamping plate 29 is attached to the outer side wall of the cell bottle 16, and the clamping plate 29 is arc-shaped matched with the outer side wall of the cell bottle 16, so that the cell bottle 16 can be clamped conveniently.

Further, a control valve 30 is arranged on the side wall of the infusion tube 7 to control the delivery of liquid nitrogen.

Further, the visual window 24 is communicated with the picking bin 5, so that the cell bottle 16 can be conveniently observed and picked up.

Working principle: when immune cells need to be stored at low temperature, the hydraulic pump 2 is started, the control valve 30 is opened, liquid nitrogen is conveyed into the cavity 6 through the liquid conveying pipe 7 to form a low-temperature storage environment, the liquid level sensor 8 is arranged, for example, when the liquid nitrogen is lower than the liquid level sensor 8, the hydraulic pump 2 is started, the liquid nitrogen is conveyed into the cavity 6, when the liquid nitrogen reaches the highest position, the hydraulic pump 2 is closed, the temperature in the box 1 can be monitored through the temperature and humidity sensor 27, for example, when the low temperature in the box 1 is lower than a specified value, the heat conducting sheet 28 starts to be heated, and when the specified value is reached, the heat conducting sheet 28 stops to be heated; before the cell bottle 16 is stored and taken out, the bulb 22 is opened, so that illumination observation is facilitated, when the angle is required to be adjusted for viewing, the air cylinder 23 is started, the inclination angle of the bulb 22 is adjusted, after the adjustment is finished, the third motor 20 is started again, the fixing plate 21 is driven to rotate, the bulb 22 is driven to rotate, and after illumination is finished, the third motor 20 is closed; when the cell bottle 16 is stored and taken out, the first motor 3 is started, the output shaft of the first motor 3 drives the threaded rod 9 to rotate, the threaded rod 9 rotates to drive the sliding plate 10 to move up and down, the sliding plate 10 drives the movable rod 12 and the mounting frame 31 to move, the movable rod 12 moves to drive the cell bottle 16 to move up and down, when the cell bottle 16 moves to the horizontal position of the storage bin 5, the first motor 3 is closed, the second motor 19 is started, the second motor 19 drives the movable rod 12 to rotate, the movable rod 12 drives the cell bottle 16 to slowly rotate, the cell bottle 16 and liquid nitrogen are in more comprehensive contact for freezing storage, meanwhile, the required cell bottle 16 is determined through the visual window 24, when the cell bottle 16 to be taken out is parallel to the storage bin 5, the electric telescopic rod 14 is started to convey the cell bottle 16 into the inner cavity of the storage bin 5 through the rotary cover plate 26, workers open the storage bin cover plate 25 to take out the cell bottle 16, and meanwhile, bacteria generated in the storage of the storage container due to the fact that the liquid nitrogen is instantaneously gasified when the container is opened to take out the cell bottle 16 are avoided, and immune cells inside the storage is destroyed.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the spirit and scope of the utility model as defined by the appended claims and their equivalents.

Claims

1. The utility model provides a low temperature storage device for immune cells, includes box (1), hydraulic pump (2), first motor (3), install bin (4), takes storehouse (5), its characterized in that: the utility model discloses a flexible electric test device, including box (1) and electric turntable (14) and is characterized in that a cavity (6) is offered to box (1) inner wall, hydraulic pump (2) is fixedly installed at box (1) top, install transfer line (7) on the infusion port of hydraulic pump (2), one end that hydraulic pump (2) was kept away from to transfer line (7) extends to in cavity (6), fixedly installed on one side inner wall of cavity (6) liquid level sensor (8), box (1) top one end fixed mounting has first motor (3), first motor (3) output is connected with threaded rod (9), threaded rod (9) are kept away from one end rotation of first motor (3) and are installed in box (1), threaded rod (9) threaded connection has slide (10), slide (10) are kept away from one end front and back of threaded rod (9) respectively alternatingly and are connected with two dead levers (11), dead lever (11) top and bottom respectively with box (1) inner chamber top and bottom fixed connection, sleeve (10) intermediate bearing are connected with movable lever (12), movable lever (12) are kept away from one end rotation of first motor (3) and are installed in box (1), two end portions (14) are fixed connection has flexible turntable (13) respectively, cell bottle (16) have been placed to test tube cover (15) inner chamber, be connected with a plurality of telescopic link (17) between test tube cover (15) inside wall and cell bottle (16) lateral wall, telescopic link (17) outside has cup jointed spring (18), movable rod (12) bottom is connected with second motor (19), second motor (19) fixed mounting is in install bin (4), slide (10) one side fixedly connected with mounting bracket (31), mounting bracket (31) bottom and install bin (4) fixed connection, install third motor (20) on box (1) top inner wall, third motor (20) output shaft has fixed plate (21), bulb (22) are articulated to fixed plate (21) bottom, one end of cylinder (23) is articulated to fixed plate (21) one side bottom, the other end and bulb (22) of cylinder (23) are articulated, box (1) one side lateral wall has seted up removable window (24), box (1) one side lateral wall is in removable window (24) position (5) are connected with take bin (5) top bin (5), the junction of box (1) and storehouse (5) of taking is connected with rotatory apron (26).

2. A cryogenic storage device for immune cells according to claim 1, characterized in that: and a temperature and humidity sensor (27) and a heat conducting fin (28) are fixedly arranged on the side wall of the inner cavity of the box body (1) respectively.

3. A cryogenic storage device for immune cells according to claim 1, characterized in that: the top of the taking bin (5) is hinged with a taking bin cover plate (25), and the joint of the box body (1) and the taking bin (5) is hinged with a rotary cover plate (26).

4. A cryogenic storage device for immune cells according to claim 1, characterized in that: the end part of the telescopic rod (17) is provided with a clamping plate (29), one side of the clamping plate (29) is attached to the outer side wall of the cell bottle (16), and the clamping plate (29) is in an arc shape matched with the outer side wall of the cell bottle (16).

5. A cryogenic storage device for immune cells according to claim 1, characterized in that: the side wall of the infusion tube (7) is provided with a control valve (30).

6. A cryogenic storage device for immune cells according to claim 1, characterized in that: the visual window (24) is communicated with the taking bin (5).