CN220564637U

CN220564637U - Cell culture reaction device

Info

Publication number: CN220564637U
Application number: CN202321914234.8U
Authority: CN
Inventors: 杨晓明; 张家友; 刘博�; 张哲罡; 张文佳; 苏敏讷; 陈光南
Original assignee: SHANGHAI RITAI MEDICINE EQUIPMENT PROJECT CO Ltd; WUHAN INSTITUTE OF BIOLOGICAL PRODUCTS CO LTD; China Biotechnology Co ltd
Current assignee: SHANGHAI RITAI MEDICINE EQUIPMENT PROJECT CO Ltd; WUHAN INSTITUTE OF BIOLOGICAL PRODUCTS CO LTD; China Biotechnology Co ltd
Priority date: 2023-07-19
Filing date: 2023-07-19
Publication date: 2024-03-08
Anticipated expiration: 2033-07-19

Abstract

The utility model relates to the technical field of cell culture devices, and solves the problems that in the prior art, a stirring device is unreasonable in design and is not beneficial to cell culture and propagation; the device comprises a tank body and a tank cover, wherein a guide cylinder along the vertical direction is arranged in the tank body, a carrier basket is arranged on the side part of the guide cylinder, a carrier unit for filling the tank body is arranged on the carrier basket, two ends of the guide cylinder are respectively communicated with the top space and the bottom space of the carrier unit, an upper magnetic stirring device extending into the upper part of the guide cylinder is arranged on the tank cover, and a lower magnetic stirring device extending into the lower part of the guide cylinder is arranged at the bottom of the tank body; the scheme is that an upper magnetic stirring device and a lower magnetic stirring device are respectively arranged at the top and the bottom of a tank body, magnetic stirring is started in the cell culture process, an upward liquid manifold is provided to enable a carrier unit to float in liquid in a static mode, the upper magnetic stirring is started in the cell digestion process, and a downward liquid manifold is provided to enable cells attached to the carrier unit to be digested.

Description

Cell culture reaction device

Technical Field

The utility model relates to the technical field of cell culture devices, in particular to a cell culture reaction device.

Background

Cell culture technology is also called cell cloning technology, in biology, normal noun is cell culture technology, and cell culture is an indispensable process in both whole bioengineering technology and biological cloning technology of one of them, cell culture is a large-scale cloning of cells, cell culture technology can be realized by culturing a large number of cells into simple single cells or few differentiated multicells, a large number of cells or metabolites thereof are obtained through cell culture, the growth of the cells needs to be carried out in a sealed culture tank, the infection of external bacterial dust is avoided, and in order to provide oxygen necessary for the growth of the cells, oxygen needs to be provided for a culture solution in the culture tank.

Cell culture equipment in the prior art, such as a biological cell culture equipment disclosed in CN213357616U, is designed and runs through the transfer line of jar body, is connected with stirring paddle on the puddler, and the transfer line drives stirring paddle and provides the liquid stirring in the stirring pipe. In the use process of the device, the cell culture carrier units are distributed in the tank body, namely between different stirring paddles, in the stirring process, the liquid flow driven by the stirring paddles can enable the cell carrier units to generate larger up-down turning, and the generated liquid vortex can cause larger damage to cells, so that the cell culture and propagation are not facilitated.

Disclosure of Invention

Based on the expression, the utility model provides a cell culture reaction device, which aims to solve the problems that the stirring device in the prior art is unreasonable in design and is unfavorable for cell culture propagation.

The technical scheme for solving the technical problems is as follows:

the utility model provides a cell cultivation reaction unit, includes the jar body and cover, the bottom of the jar body is provided with the discharge gate, the inside draft tube that is provided with along vertical direction of jar, the lateral part of draft tube is provided with the carrier basket, be provided with the carrier unit who fills the jar body on the carrier basket, the top and the bottom space of carrier unit are communicated respectively at the both ends of draft tube, be provided with the last magnetic stirring device that stretches into to the draft tube top on the cover, the bottom of the jar body is provided with the lower magnetic stirring device that stretches into to the draft tube below.

On the basis of the technical scheme, the utility model can be improved as follows.

Further, go up magnetic stirring device includes first drive arrangement, first (mixing) shaft and first stirring subassembly, first drive arrangement sets up the axle center department on the cover, first (mixing) shaft sets up in first drive arrangement's bottom, first stirring subassembly sets up in first (mixing) shaft's bottom, first stirring subassembly includes a plurality of circumference distributed churn, churn perpendicular to first (mixing) shaft and outwards extend, churn is hollow drum.

Further, magnetic stirring device includes second drive arrangement, second (mixing) shaft and second stirring subassembly down, second drive arrangement sets up in the bottom of jar body, the second (mixing) shaft is connected in second drive arrangement's output, second stirring subassembly is connected in the top of second (mixing) shaft, second stirring subassembly includes a plurality of circumferentially distributed paddles.

Further, a surface layer air inlet, a sight lamp, a rupture disc opening, a jacket water outlet, a liquid transferring opening, a material supplementing valve opening, a sight glass and a pressure gauge opening are arranged on the side wall of the tank body above the carrier basket.

Further, a sampling valve port, a thermometer interface, a process interface, a PH electrode interface, a DO electrode interface and a deep air inlet are arranged on the side wall of the tank body below the carrier basket.

Further, an open annular jacket cylinder is sleeved on the outer circumference of the tank body, the jacket cylinder comprises a cylinder steel plate arranged along the circumference of the tank body, and the jacket cylinder further comprises a guide plate positioned on the outer circumference of the tank body.

Further, a liquid level gauge with the same height as the highest part of the jacket cylinder body is arranged on the outer circumference of the tank body.

Compared with the prior art, the technical scheme of the application has the following beneficial technical effects:

this scheme is internal to be gone up in the jar through carrier basket and draft tube with jar internal portion about three district, the cell cultivates in the district of carrier basket, carrier basket top is the upper region, carrier basket below is the lower zone, through draft tube intercommunication upper region and lower zone, set up magnetic stirring device and lower magnetic stirring device's double stirring respectively with the bottom at jar top of the body, magnetic stirring under the culture process start of cell, provide ascending liquid manifold and make carrier unit static float in liquid, start magnetic stirring at the cell digestion in-process, provide decurrent liquid manifold and make the cell digestion that adheres to in carrier unit get off, transfer through the discharge gate of tank bottom.

Drawings

FIG. 1 is a schematic diagram of a cell culture reaction apparatus according to the present utility model;

FIG. 2 is an enlarged view of a portion of the area of FIG. 1;

FIG. 3 is a cross-sectional view taken at A-A of FIG. 1;

FIG. 4 is a cross-sectional view taken at B-B of FIG. 1;

in the drawings, the list of components represented by the various numbers is as follows:

1. a tank body; 2. a can lid; 3. a level gauge; 4. a discharge port; 5. a guide cylinder; 6. a carrier basket; 7. a first driving device; 8. a first stirring shaft; 9. a first stirring assembly; 10. a second driving device; 11. a second stirring shaft; 12. a second stirring assembly; 13. a surface layer air inlet; 14. a vision lamp; 15. rupture disc ports; 16. a jacket water outlet; 17. a pipetting port; 18. a material supplementing valve port; 19. a viewing mirror; 20. a pressure gauge port; 21. a sampling valve port; 22. a thermometer interface; 23. a process interface; 24. a PH electrode interface; 25. a DO electrode interface; 26. a deep air inlet; 27. a jacket cylinder; 28. and a deflector.

Detailed Description

In order to facilitate an understanding of the present application, a more complete description of the present application will now be provided with reference to the relevant figures. Examples of the present application are given in the accompanying drawings. This application may, however, be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

It will be appreciated that spatially relative terms such as "under …," "under …," "below," "under …," "over …," "above," and the like may be used herein to describe one element or feature's relationship to another element or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use and operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements or features described as "under" or "beneath" other elements would then be oriented "on" the other elements or features. Thus, the exemplary terms "under …" and "under …" may include both an upper and a lower orientation. Furthermore, the device may also include an additional orientation (e.g., rotated 90 degrees or other orientations) and the spatial descriptors used herein interpreted accordingly.

It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or be connected to the other element through intervening elements. In the following embodiments, "connected" is understood to mean "electrically connected", "communicatively connected", and the like, if the connected circuits, modules, units, and the like have electrical or data transferred therebetween.

As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and/or "comprising," and/or the like, specify the presence of stated features, integers, steps, operations, elements, components, or groups thereof, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or groups thereof.

The cell culture reaction device shown in fig. 1 and 2 comprises a tank body 1 and a tank cover 2, wherein a dish-shaped sealing head is sleeved at the bottom of the tank body 1, a discharge port 4 for discharging the cell tank is arranged at the bottom of the dish-shaped sealing head, and a tank bottom diaphragm valve and a discharge valve are arranged at the discharge port 4. An open-ring-shaped jacket cylinder 27 is sleeved on the outer circumference of the tank body 1, the jacket cylinder 27 comprises a cylinder steel plate arranged along the circumference of the tank body 1, and the cylinder steel plate and the tank body 1 are fixed in a lateral sealing manner through a jacket in the vertical direction. The jacket cylinder 27 further comprises a deflector 28 located on the outer circumference of the tank 1. A liquid level gauge 3 having the same height as the jacket cylinder 27 is further provided on the outer circumference of the tank 1.

The inside draft tube 5 that is provided with along vertical direction of jar body 1, the lateral part of draft tube 5 is provided with carrier basket 6, and carrier basket 6 is placed on installing the inside tray of jar body 1, is provided with the carrier unit of filling jar body 1 on the carrier basket 6, and the carrier unit reserves has the nutrient to be used for cultivating the cell. The two ends of the guide cylinder 5 are respectively communicated with the top space and the bottom space of the carrier unit, an upper magnetic stirring device extending into the upper part of the guide cylinder 5 is arranged on the tank cover 2, and a lower magnetic stirring device extending into the lower part of the guide cylinder 5 is arranged at the bottom of the tank body 1. The upper magnetic stirring device provides a top-to-bottom liquid manifold, and the lower magnetic stirring device provides a bottom-to-top liquid manifold.

Go up magnetic stirring device and include first drive arrangement 7, first (mixing) shaft 8 and first (mixing) module 9, first drive arrangement 7 sets up the axle center department on cover 2, first drive arrangement 7 is including setting up in cover 2 regular quick-witted seal interface, quick-witted seal seat and sealing washer, and driving motor and reduction gear, first (mixing) shaft 8 passes cover 2 downwardly extending to jar in 1, first (mixing) module 9 has been cup jointed in the bottom of first (mixing) shaft 8, first (mixing) module 9 includes a plurality of circumference distributed churn, churn perpendicular to first (mixing) shaft 8 and outwards extend, the churn is hollow drum. The lower magnetic stirring device comprises a second driving device 10, a second stirring shaft 11 and a second stirring assembly 12, wherein the second driving device 10 is arranged at the bottom of the tank body 1 and also comprises a corresponding sealing seat, a motor and a speed reducer, the second stirring shaft 11 is connected to the output end of the second driving device 10, the second stirring assembly 12 is connected to the top end of the second stirring shaft 11, and the second stirring assembly 12 comprises a plurality of circumferentially distributed paddles.

Lower magnetic stirring was used for the cell culture growth phase. In the cell growth phase, it is desirable that the cells in the carrier unit have sufficient nutrient exchange while minimizing shear damage thereto. The upward liquid flow shape provided by the lower magnetic stirring allows the carrier unit to float still in the liquid, facilitating the growth of cells attached to the carrier. The upper magnetic stirring was used for the later cell digestion stage. In the latter stage of cell culture, the cells adhered to the carrier are digested and transferred through the discharge port 4 at the bottom of the tank, and the downward liquid manifold provided by the upper magnetic stirring can allow the digested cells to be transferred through the outlet of the tank bottom along with the fluid passing through the carrier basket 6 downwards under the stirring action.

The side wall of the tank body 1 above the carrier basket 6 is provided with a surface air inlet 13, a sight lamp 14, a rupture disc port 15, a jacket water outlet 16, a liquid transfer port 17, a material supplementing valve port 18, a sight glass 19 and a pressure gauge port 20.

The side wall of the tank body 1 below the carrier basket 6 is provided with a sampling valve port 21, a thermometer interface 22, a process interface 23, a PH electrode interface 24, a DO electrode interface 25 and a deep air inlet 26.

The foregoing description of the preferred embodiments of the utility model is not intended to limit the utility model to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the utility model are intended to be included within the scope of the utility model.

Claims

1. The utility model provides a cell cultivation reaction unit, includes the jar body and cover, the bottom of the jar body is provided with the discharge gate, its characterized in that, the inside draft tube that is provided with along vertical direction of jar, the lateral part of draft tube is provided with the carrier basket, be provided with the carrier unit who fills the jar body on the carrier basket, the top and the bottom space of carrier unit are linked together respectively at the both ends of draft tube, be provided with the last magnetic stirring device who stretches into to the draft tube top on the cover, the bottom of the jar body is provided with the lower magnetic stirring device who stretches into to the draft tube below.

2. The cell culture reaction device according to claim 1, wherein the upper magnetic stirring device comprises a first driving device, a first stirring shaft and a first stirring assembly, the first driving device is arranged at the axle center of the tank cover, the first stirring shaft is arranged at the bottom end of the first driving device, the first stirring assembly is arranged at the bottom end of the first stirring shaft, the first stirring assembly comprises a plurality of stirring cylinders distributed circumferentially, the stirring cylinders are perpendicular to the first stirring shaft and extend outwards, and the stirring cylinders are hollow cylinders.

3. The cell culture reaction device according to claim 1, wherein the lower magnetic stirring device comprises a second driving device, a second stirring shaft and a second stirring assembly, the second driving device is arranged at the bottom of the tank body, the second stirring shaft is connected to the output end of the second driving device, the second stirring assembly is connected to the top end of the second stirring shaft, and the second stirring assembly comprises a plurality of blades distributed circumferentially.

4. The cell culture reaction device according to claim 1, wherein a surface layer air inlet, a sight lamp, a rupture disc port, a jacket water outlet, a liquid transfer port, a material supplementing valve port, a sight glass and a pressure gauge port are arranged on the side wall of the tank body above the carrier basket.

5. The cell culture reaction device according to claim 1, wherein a sampling valve port, a thermometer interface, a process interface, a PH electrode interface, a DO electrode interface and a deep air inlet are arranged on the side wall of the tank body below the carrier basket.

6. The cell culture reaction device according to claim 1, wherein an open-loop jacket cylinder is sleeved on the outer circumference of the tank body, the jacket cylinder comprises a cylinder steel plate arranged along the circumference of the tank body, and the jacket cylinder further comprises a guide plate positioned on the outer circumference of the tank body.

7. The cell culture reaction apparatus according to claim 6, wherein a liquid level gauge having the same height as the highest position of the jacket cylinder is further provided on the outer circumference of the tank.