CN220514212U - Pipetting suction head with low CV value - Google Patents
Pipetting suction head with low CV value Download PDFInfo
- Publication number
- CN220514212U CN220514212U CN202321928531.8U CN202321928531U CN220514212U CN 220514212 U CN220514212 U CN 220514212U CN 202321928531 U CN202321928531 U CN 202321928531U CN 220514212 U CN220514212 U CN 220514212U
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- Prior art keywords
- pipette
- low
- pipetting
- value
- suction head
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- 239000000463 material Substances 0.000 claims abstract description 24
- 238000007789 sealing Methods 0.000 claims abstract description 13
- 238000007747 plating Methods 0.000 claims abstract description 7
- 239000004743 Polypropylene Substances 0.000 claims abstract description 6
- -1 polypropylene Polymers 0.000 claims abstract description 6
- 229920001155 polypropylene Polymers 0.000 claims abstract description 6
- 239000011248 coating agent Substances 0.000 claims abstract description 5
- 238000000576 coating method Methods 0.000 claims abstract description 5
- 238000005498 polishing Methods 0.000 claims description 14
- 238000000227 grinding Methods 0.000 claims description 6
- 238000005229 chemical vapour deposition Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000005240 physical vapour deposition Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 238000001179 sorption measurement Methods 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 13
- 230000006978 adaptation Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000005260 corrosion Methods 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000002310 reflectometry Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Abstract
The utility model relates to the technical field of laboratory vessel burets, in particular to a low CV value pipetting tip which comprises an assembly cavity tube, a pipette and a pipetting tip, wherein the lower surface of the assembly cavity tube and the upper surface of the pipetting tip are fixedly connected with the upper surface and the lower surface of the pipette respectively, and DLC plating layers are uniformly plated on the inner walls of the assembly cavity tube and the pipette; the structure of the assembly cavity tube comprises an adaptation tube, wherein the middle part of the inner wall of the adaptation tube is fixedly connected with a plurality of sealing plug rings, and the adaptation tube, a pipette and a pipetting head are made of the same materials; in the pipetting suction head with the low CV value, the DLC coating is uniformly plated on the inner wall of the pipetting suction head, the sealing plug ring is arranged in the adapting pipe, and the modified polypropylene material with low adsorptivity is adopted, so that the functions of reducing volume variation and distribution error when the suction head is used for sucking liquid are realized, and the effects of low adsorptivity and low residual quantity are achieved.
Description
Technical Field
The utility model relates to the technical field of laboratory vessel burets, in particular to a pipetting tip with a low CV value.
Background
The suction head is a tool for pipetting, sucking, distributing and transferring liquid in the fields of laboratory, medical treatment, industry and the like, and at present, a part of liquid can not be completely discharged all the time in the titration process using the suction head, and remains in the suction head, especially when an experiment with high accuracy and sensitivity is performed, a certain error result and a problem of reagent waste are caused, and a CV value refers to a variation coefficient for measuring the discrete degree of a sample test result, so that how to reduce the volume variation and distribution error during liquid suction, and therefore, the improvement of the accuracy and the repeatability of an analysis result are the key for improving the lower residual rate of the suction head.
For example, application number cn201822152. X discloses a high-precision pipette tip, including an upper end opening, an upper wall portion, a lower wall portion, a tip opening, a single chamfer, a double chamfer and a connecting tube structure, by improving the shape of the pipette tip, i.e., designing the tip opening to have a tip opening with a single chamfer or a double chamfer for reducing bead hanging residue, improving the accuracy of the pipette tip, but for a liquid with high viscosity or a large surface tension, there is a defect that adhesion phenomenon is more likely to occur in the tip opening, resulting in incomplete discharge or difficult discharge of the liquid, and cleaning of the tip opening, resulting in cross contamination of samples that occurs incompletely with cleaning and affecting the accuracy of experimental results; the application number is CN202221560835.9 discloses among the pipetting tip, including the tip, the pipette, positioning platform, assembly chamber, bellying and bayonet part structure, through utilizing the bump assembly of tip and pipette, make the plug power of tip and pipette little, be used for pulling out convenient, leakproofness and durability good, and through utilizing positioning platform to fix a position the depth of insertion of pipette, be used for guaranteeing that the depth of insertion of every pipette is unanimous, but there is the adsorptivity of tip material high, the wall thickness is great to lead to the reagent residual quantity high, and the leakproofness is poor to lead to the gaseous defect that has appeared the volume variation that gets into.
Therefore, both of the above patent utility models disclose pipette tips, but there are still variations in volume and dispensing errors during pipetting, and the problem of high reagent residue caused by high adsorptivity and large wall thickness of the produced tip material.
Disclosure of Invention
The utility model aims to provide a pipetting tip with a low CV value, which solves the technical problems mentioned in the background art.
In order to achieve the above purpose, the utility model discloses a low CV value pipetting tip, which comprises an assembling cavity tube, a pipette and a pipetting tip, wherein the lower surface of the assembling cavity tube and the upper surface of the pipetting tip are fixedly connected with the upper surface and the lower surface of the pipette respectively, and DLC plating layers are uniformly plated on the inner walls of the assembling cavity tube and the pipette; the structure of the assembly cavity tube comprises an adapting tube, and a plurality of sealing plug rings are fixedly connected to the middle of the inner wall of the adapting tube.
Optionally, the adapting tube, the pipette and the pipette tip are made of the same material and are made of low-adsorption modified polypropylene materials, so that the cleaning and maintenance convenience is improved.
Optionally, the DLC coating is prepared by a physical vapor deposition or chemical vapor deposition process, and has the characteristics of high hardness, low friction coefficient, chemical inertness, biocompatibility and transparency, for optimizing pipetting accuracy to 0.1ul, so that the error range of each liquid transfer can be controlled within 0.1 ul.
Optionally, the inner wall of the liquid-transfering suction head adopts polishing material to polish the liquid-transfering suction head, so that the smoothness of the inner wall of the liquid-transfering suction head reaches Ra0.01, and the inner wall of the liquid-transfering suction head is used for controlling the average roughness of the inner surface of the liquid-transfering suction head to be 0.01 microns, so that the inner wall of the liquid-transfering suction head has no obvious concave-convex and flaws, and has the characteristics of low friction resistance, high reflectivity and good corrosion resistance; the polishing material is any one of polishing liquid, grinding paste and polishing tool, and adopts rotation, friction and other means to polish, so as to further improve the smoothness of the inner wall of the pipetting head.
Optionally, the bottom of the pipette tip is a flat opening, the caliber of the pipette tip is less than or equal to 0.6mm, the wall thickness of the pipette tip from the upper caliber to the lower caliber is gradually thinned, and the pipette tip is used for enabling the wall thickness of the pipette tip to be inversely proportional to pipetting accuracy, and the pipette tip has the characteristics that the thinner the inner wall is, the smaller the CV value is.
Optionally, the material of sealing ring is silica gel for sealing connection pipettes adapter, reduces the inside air volume of suction head when installing, is used for reducing the mixture and the violent fluctuation of liquid and gas, thereby reduces the CV value.
Optionally, the thickness of the adapting tube is 0.5-0.8mm, and the thickness is directly proportional to the pipetting accuracy, so as to maintain the tightness of the adapting tube.
Advantageous effects
Compared with the prior art, the utility model has the following advantages:
1. in the pipetting suction head with the low CV value, the DLC coating is uniformly plated on the inner wall of the pipetting suction head, the sealing plug ring is arranged in the adapting pipe, and the modified polypropylene material with low adsorptivity is adopted, so that the functions of reducing volume variation and distribution error when the suction head is used for sucking liquid are realized, and the effects of low adsorptivity and low residual quantity are achieved.
Drawings
FIG. 1 is a schematic view of the elevation view of a low CV value pipette tip of the present utility model.
FIG. 2 is a schematic diagram of a low CV value pipette tip of the present utility model in a side elevational view.
FIG. 3 is a cross-sectional view showing the longitudinal structure of a low CV value pipette tip of the present utility model.
The reference numerals are: 1. assembling a cavity tube; 101. adapting the pipe; 102. sealing the plug ring; 2. a pipette; 3. pipetting tips; 4. DLC plating.
Detailed Description
The technical scheme of the utility model is explained in detail by specific examples.
Referring to FIGS. 1-3, the utility model discloses a pipetting tip structure with low CV value, which comprises an assembling cavity tube 1, a pipette 2 and a pipetting tip 3, wherein the lower surface of the assembling cavity tube 1 and the upper surface of the pipetting tip 3 are fixedly connected with the upper surface and the lower surface of the pipette 2 respectively, and DLC plating layers 4 are uniformly plated on the inner walls of the assembling cavity tube 1 and the pipette 2; the structure of the assembly cavity tube 1 comprises an adapting tube 101, and a plurality of sealing plug rings 102 are fixedly connected to the middle part of the inner wall of the adapting tube 101.
In a preferred embodiment, the adapting tube 101, the pipette 2 and the pipette tip 3 are made of the same material and are made of a low-adsorption modified polypropylene material, and the material has the characteristics of improving the anti-adsorption property, increasing the smoothness of the material surface and improving the convenience of cleaning and maintenance, and is used for optimizing the material of the tip and reducing the adsorptivity of the traditional polypropylene material.
In a preferred embodiment, the DLC coating 4 is produced by a physical vapor deposition or chemical vapor deposition process, which has the characteristics of high hardness, low friction coefficient, chemical inertness, biocompatibility and transparency, for optimizing the pipetting accuracy to 0.1ul, so that the error range per liquid transfer can be controlled within 0.1ul, improving the pipetting accuracy while ensuring the accuracy and reliability of the experimental results.
In a preferred embodiment, the inner wall of the pipette tip 3 is polished by adopting a polishing material, so that the smoothness of the inner wall of the pipette tip 3 reaches Ra0.01, and the inner wall of the pipette tip 3 is used for controlling the average roughness of the inner surface of the pipette tip 3 to be 0.01 micrometers, so that the inner wall of the pipette tip is free from obvious concave-convex and flaw, and has the characteristics of low friction resistance, high reflectivity and good corrosion resistance; the polishing material is any one of polishing liquid, grinding paste and polishing tool, and adopts rotation, friction and other means to polish, so as to further improve the smoothness of the inner wall of the pipetting head 3 and remove fine flaws and scratches.
In a preferred embodiment, the bottom end of the pipette tip 3 is a flat opening, the caliber of the bottom end is less than or equal to 0.6mm, the wall thickness of the pipette tip 3 from the upper caliber to the lower caliber is gradually reduced, the wall thickness of the pipette tip 3 is inversely proportional to pipetting accuracy, the thinner the inner wall is, the smaller the CV value is, the less reagent residue in the tip is, and the experiment using the pipette tip test is better in stability and accuracy.
In a preferred embodiment, the sealing ring 102 is made of silica gel for sealing the pipette adapter, reducing the air volume inside the tip during installation, reducing mixing and severe fluctuations of liquid and gas, and thus reducing CV values.
In a preferred embodiment, the adapter tube 101 has a thickness of 0.5-0.8mm, which is proportional to the pipetting accuracy for maintaining the tightness of the adapter tube.
Working principle: firstly, cleaning and low-temperature drying are carried out on a pipette head formed by an assembly cavity tube 1, a pipette 2 and a pipette head 3, grinding is carried out on the inner wall of the pipette head 3 by using a grinding material, polishing treatment is carried out on the inner wall of the pipette head 3 by using a polishing material after grinding, after cleaning and low-temperature drying again, DLC plating 4 is uniformly plated on the inner wall of the pipette head 3 by adopting physical vapor deposition or chemical vapor deposition, so that the DLC plating 4 has low adsorptivity, then the inner movable sleeve on the upper surface of an adaptation tube 101 is sleeved at the bottom end of the adaptation pipette part, the sealing plug ring 102 is matched with the outer wall of the adaptation pipette part, and the air containing amount in the pipette head during installation is reduced, so that CV value is reduced, and the lower caliber of the pipette head 3 is used for absorbing reagent.
The above description is only of the preferred embodiments of the present utility model, and is not intended to limit the utility model, but any modifications, equivalent substitutions, improvements, etc. within the design concept of the present utility model should be included in the scope of the present utility model.
Claims (7)
1. A low CV value pipette tip, characterized in that: the automatic pipetting device comprises an assembly cavity tube (1), a pipette (2) and a pipetting tip (3), wherein the lower surface of the assembly cavity tube (1) and the upper surface of the pipetting tip (3) are fixedly connected with the upper surface and the lower surface of the pipette (2) respectively, and DLC plating layers (4) are uniformly plated on the inner walls of the assembly cavity tube (1) and the pipette (2); the structure of the assembly cavity tube (1) comprises an adapting tube (101), and a plurality of sealing plug rings (102) are fixedly connected to the middle of the inner wall of the adapting tube (101).
2. A low CV value pipette tip as defined in claim 1, wherein: the adapting pipe (101), the pipette (2) and the pipette tip (3) are made of the same material and are made of low-adsorption modified polypropylene materials.
3. A low CV value pipette tip as defined in claim 1, wherein: the DLC coating (4) is prepared by a physical vapor deposition or chemical vapor deposition process.
4. A low CV value pipette tip as defined in claim 1, wherein: the inner wall of the liquid-transfering suction head (3) is polished by adopting a polishing material, so that the smoothness of the inner wall of the liquid-transfering suction head (3) reaches Ra0.01; the polishing material is selected from any one of polishing solution, grinding paste and polishing tool.
5. A low CV value pipette tip as defined in claim 1, wherein: the bottom end of the liquid-transfering suction head (3) is a flat opening, the caliber of the liquid-transfering suction head is less than or equal to 0.6mm, and the wall thickness of the liquid-transfering suction head (3) from the upper caliber to the lower caliber is gradually thinned.
6. A low CV value pipette tip as defined in claim 1, wherein: the sealing plug ring (102) is made of silica gel.
7. A low CV value pipette tip as defined in claim 1, wherein: the thickness of the adapting pipe (101) is 0.5-0.8mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321928531.8U CN220514212U (en) | 2023-07-21 | 2023-07-21 | Pipetting suction head with low CV value |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321928531.8U CN220514212U (en) | 2023-07-21 | 2023-07-21 | Pipetting suction head with low CV value |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220514212U true CN220514212U (en) | 2024-02-23 |
Family
ID=89938972
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321928531.8U Active CN220514212U (en) | 2023-07-21 | 2023-07-21 | Pipetting suction head with low CV value |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220514212U (en) |
-
2023
- 2023-07-21 CN CN202321928531.8U patent/CN220514212U/en active Active
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant |