CN2204809Y - 一种生物发酵液分析仪之酶电极 - Google Patents

一种生物发酵液分析仪之酶电极 Download PDF

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CN2204809Y
CN2204809Y CN 94229256 CN94229256U CN2204809Y CN 2204809 Y CN2204809 Y CN 2204809Y CN 94229256 CN94229256 CN 94229256 CN 94229256 U CN94229256 U CN 94229256U CN 2204809 Y CN2204809 Y CN 2204809Y
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enzyme
electrodes
electrode
probe head
probe
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杨庆玲
邹宏声
毕可万
王瑞光
张宇
王乐群
王霜鹤
曲军
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Dalian Institute of Chemical Physics of CAS
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Dalian Institute of Chemical Physics of CAS
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Abstract

本装置属于分析仪之探头部件,其结构由氧化氢 探头和酶膜构成,外面为聚砜圆筒,筒内装有铂电极 和两个银电极,用环氧树脂封住,电极分别和引线引 出,下部装有酶膜固定圈,内装酶膜,之间为充有电解 液空隙,端部为球面形,里面装有内层膜,外层为支撑 层,将铂电极加0.7伏电位测定生成H202氧化电 流。优点:结构简单,测量速度快,精度高,工作性能 稳定,生产容易,成本低,可推广使用。

Description

本实用新型属于一种分析仪探头部件。
目前,在以对糖类、有机酸、醇类的分析多采用化学法、酶法和色谱法。目前多采用化学方法对工业发酵液的分析,其缺点:操作麻烦,耗资大,耗时长,误差大,其杂质的干扰往往影响测量结果。为解决对工业发酵液的分析,近年常采用流动注射型酶电极分析仪以对各种工业发酵液检测,以采用氧化酶电极为检测元件,以安装电极数目的不同分为单电极系统和双电极系统。当采用氧化酶酶电极检测底物,使用H2O2探头灵敏度高,但酶膜不仅要排除大分子量的干扰物,而且要能排除小分子量的电极活性干扰物的影响,按照上述要求制造的酶电极是将醋酸纤维素配成环乙铜溶液,水上成膜,用聚乙烯板托出,晾干;将覆盖粘结层材料配在溶液或调成糊状,加到醋酸纤维素膜上,用聚碳酸脂膜覆盖,形成叠层酶膜,其缺点是水上成膜,工艺难度大,酶-粘结层的组成浪费酶量大,有时粘结不牢,要求电解池结构复杂,采用Ag/AgCe电极有一定的寿命,定期填加补充电解液不便。
为解决以上酶电极之不足,本实用新型提供一种分析仪之酶电极,利用氧化氢探头和酶膜构成,以达到测量速度快,性能稳定,抗干扰性强的目的。
本实用新型的结构设计结合实施例加以说明。
图1为生物发酵液分析仪之酶电极结构原理图;
图1为生物发酵液分析仪之酶电极正投影图。
其结构结合附图加以说明,该酶电极其结构由过氧化氢探头和酶膜构成,其中过氧化氢探头是在聚砜(或有机玻璃)管1内装有铂电极2和两个半环状银电极3和4,三个电极用环氧树脂5封在管1内,6、7、8为电极引线,在聚砜管1下部外面装有聚四氟乙烯的酶膜定位圈9,定位圈内为酶膜圈14,就装在定位圈内,探头端部加工成球面形,以保证酶膜和探头表面接触良好,10为酶膜14和探头之间的间隙,充满了电解液,是一个很薄的液层,11为内层膜,12为活性层,13为外层支撑膜,酶电极以一个压帽固定到检测池上进行测量,电极引线6接恒电位源的工作电极,7、8分别接到参考电极和辅助电极上,给铂电极加0.7伏(相对于银参考电极)电位,测定酶促反应生成的H2O2的氧化电流,此电流作为分析仪测定生物发酵酶的重要性能参数。
本实用新型之优点:此酶电极测量速度快,精度高,工作性能稳定,容易生产,成本低,可推广使用。

Claims (2)

1、一种生物发酵液分析仪之酶电极,其特征是该酶电极其结构由过氧化氢探头和酶膜构成,其中过氧化氢探头是在聚砜(或有机玻璃)管(1)内装有铂电极(2)和两个半环状银电极(3)和(4),三个电极用环氧树脂(5)封在管(1)内,(6)、(7)、(8)为电极引线,在聚砜管(1)下部外面装有聚四氟乙烯的酶膜定位圈(9),定位圈内为酶膜圈(14),就装在定位圈(9)内,探头端部为球面形,酶膜(14)与探头之间为间隙(10),充满了电解液,是一个很薄的液层,(11)为内层膜,(12)为活性层,(13)为外层支撑膜。
2、按权利要求1所述之酶电极,其特征在于酶电极以一个压帽固定在检测池,电极引线(6)接恒电位源的工作电极,(7)、(8)分别接到参考电极和辅助电极上,给铂电极加0.7伏(相对于银电极)电位,测定酶促反应生成的H2O2的氧化电流。
CN 94229256 1994-07-30 1994-07-30 一种生物发酵液分析仪之酶电极 Expired - Fee Related CN2204809Y (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101140259B (zh) * 2007-10-11 2010-12-22 福州大学 毛细管电泳-热工作电极-安培检测装置及热工作电极制作方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101140259B (zh) * 2007-10-11 2010-12-22 福州大学 毛细管电泳-热工作电极-安培检测装置及热工作电极制作方法

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