CN220460714U - Liquid drop positioning detection device of photoinduction dielectric wetting chip - Google Patents
Liquid drop positioning detection device of photoinduction dielectric wetting chip Download PDFInfo
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- CN220460714U CN220460714U CN202321777153.8U CN202321777153U CN220460714U CN 220460714 U CN220460714 U CN 220460714U CN 202321777153 U CN202321777153 U CN 202321777153U CN 220460714 U CN220460714 U CN 220460714U
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- 239000007788 liquid Substances 0.000 title claims abstract description 60
- 238000009736 wetting Methods 0.000 title claims abstract description 43
- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 229920002545 silicone oil Polymers 0.000 claims abstract description 9
- 230000000694 effects Effects 0.000 claims abstract description 5
- 239000011521 glass Substances 0.000 claims description 14
- 230000002209 hydrophobic effect Effects 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 claims description 2
- 238000005286 illumination Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
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Abstract
The utility model discloses a liquid drop positioning detection device of a photoinduction dielectric wetting chip, which belongs to the field of digital microfluidics, and comprises the following components: the device comprises a photoinduced dielectric wetting chip, black silicone oil filled in the chip, liquid drops to be operated, a liquid drop positioning detection device and an infrared light source; the utility model achieves the technical effects that: after the upper polar plate and the lower polar plate of the chip are electrified with alternating voltage with certain frequency and amplitude, liquid drops in the chip can be driven by light spots irradiated on the lower polar plate of the chip by an infrared light source, and meanwhile, infrared light passing through the liquid drops can be received by a liquid drop positioning detection device, so that the function of detecting the positions of the liquid drops in real time is achieved.
Description
Technical Field
The utility model relates to the field of digital microfluidics, in particular to a liquid drop positioning detection device of a photoinduction dielectric wetting chip.
Background
The Digital micro-fluidic (Digital MicroFluidics, DMF) technology is a novel technology for manipulating discrete liquid drops, can precisely control the discrete liquid drops through dielectric wetting, magnetic force and the like, and has wide application prospects in the aspects of nucleic acid amplification, cell culture, chemical reaction and the like.
At present, in the application of the digital microfluidic field, a dielectric wetting principle or a photo-induced dielectric wetting principle is mostly adopted to control the liquid drop, and the liquid drop needs to be positioned in the process of controlling the liquid drop.
Disclosure of Invention
The utility model aims at solving the problem that the cost is high because a high-precision camera or a microscope is adopted for positioning liquid drops by a traditional photoinduction dielectric wetting chip, and discloses a liquid drop positioning detection device of the photoinduction dielectric wetting chip, which comprises the following components:
the liquid drop positioning detection device of the photoinduced dielectric wetting chip is characterized by comprising:
the structure of the photoinduced dielectric wetting chip can be divided into an upper part and a lower part, and the lower half part of the structure of the photoinduced dielectric wetting chip is sequentially from bottom to top: the upper half part of the structure of the photoinduction dielectric wetting chip is sequentially provided with a hydrophobic layer and ITO conductive glass from bottom to top; packaging the upper part and the lower part of the structure of the photoinduced dielectric wetting chip by using a double-sided tape to form a closed cavity for droplet operation;
the ITO conductive glass at the upper part of the photoinduced dielectric wetting chip structure and the ITO conductive glass at the lower part of the photoinduced dielectric wetting chip structure can be applied with alternating current with certain amplitude and frequency to charge the photoinduced dielectric wetting chip;
a liquid comprising a droplet and a black silicone oil, in a chamber for droplet operation, sandwiched between a hydrophobic layer in an upper portion of the photo-induced dielectric wetting chip structure and a hydrophobic layer in a lower portion of the photo-induced dielectric wetting chip structure;
the liquid drop positioning detection device is positioned right above the photoinduced dielectric wetting chip and consists of a photodiode array and a processor, wherein the size of the photodiode array is consistent with the length and the width of the chip;
the purple LED is used for generating light spots to drive the liquid drops, and light transmitted through the liquid drops is received by the liquid drop positioning detection device, so that liquid drop positioning is realized.
In the improved scheme, the liquid drop positioning detection device consists of a photodiode array and a processor, wherein the size of the photodiode array is consistent with the length and the width of a chip;
the photodiode array is formed by a plurality of photodiodes, and is used for receiving the light rays which are emitted from the purple LED and penetrate through the liquid drops and realizing photoelectric conversion;
the processor is electrically connected with the photodiode array, and is used for receiving the electric signals sent by the photodiode array and judging the positions of the liquid drops according to the positions of the photodiodes, which receive the optical signals, in the photodiode array.
In a modified solution, the chamber for droplet manipulation is filled with black silicone oil, which surrounds the droplets, so that the light emitted by the violet LED can be received by the photodiode array only through a portion of the droplets.
In the improved scheme, after alternating current with a certain amplitude and frequency is supplied to ITO conductive glass of the upper and lower parts of the structure of the photoinduced dielectric wetting chip, a light spot generated by a purple LED changes the conductive characteristic of a photoconductive layer, when the light spot irradiates on one side of a liquid drop, a dielectric electrowetting effect can occur on the one side, so that the contact angle between the edge of the liquid drop on the irradiated side and a hydrophobic layer is reduced, and a pressure difference is generated inside the liquid drop, so that the liquid drop moves towards the irradiation direction of an infrared light source, and the phenomenon is also called the photoelectric electrowetting effect.
The beneficial effects of the utility model are as follows:
the droplet may be driven by the electrowetting effect and as the violet LED spot moves, the droplet may be driven to any location in the chip.
The black silicone oil surrounding the drop can ensure that only the violet LED light passing through the drop can be received by the photodiode array directly above the chip.
When the purple LED light passing through the liquid drop is received by the photodiode array, the output voltage of the photodiode can change, the processor can receive the electric signal, the real-time positioning of the liquid drop is realized, and the accurate movement of the liquid drop to the place where the liquid drop is required to arrive is ensured.
Drawings
FIG. 1 is a schematic diagram of a photo-induced dielectric wetting device and a droplet placement detection device according to the present application;
FIG. 2 is a schematic diagram of the photodiode array of FIG. 1;
FIG. 3 is a schematic diagram of the purple LED of FIG. 1 emitting light to drive a droplet.
Reference numerals:
1-photo-induced dielectric wetting chip body; 10-ITO conductive glass; 11-a light guiding layer; 12-a dielectric layer; 13-a hydrophobic layer; 14-a tape layer; 2-liquid in the chip; 20-droplets to be handled; 21-black silicone oil; a 3-violet LED light source; 4-a droplet positioning detection device; 41-photodiode array; 410-a photodiode; 42-processor.
Detailed Description
The present utility model will be described in detail with reference to the accompanying drawings.
Embodiment 1 As shown in fig. 1, as a specific embodiment of the utility model, a liquid drop positioning detection device of a photo-induced dielectric wetting chip is disclosed, which is used in the field of digital microfluidics, and comprises a photo-induced dielectric wetting chip 1, an intra-chip liquid 2, a purple LED light source 3 and a liquid drop positioning detection device 4.
As shown in fig. 1, the structure of the light-induced dielectric wetting chip may be divided into an upper half and a lower half, where the lower half is sequentially from bottom to top: the ITO conductive glass 10, the photoconductive layer 11, the dielectric layer 12 and the hydrophobic layer 13, wherein the upper half part is sequentially provided with the hydrophobic layer 13 and the ITO conductive glass 10 from bottom to top; the upper and lower parts are connected and fixed by double-sided tape 14 to form a closed chamber for droplet operations.
As shown in fig. 1, the droplet positioning detection device 4 is located right above the upper polar plate of the photoinduced dielectric wetting chip 1, and is composed of a photodiode array 41 and a processor 42; as shown in fig. 2, the photodiode array is composed of photodiodes 410, the size of the photodiode array is the same as that of the chip, the photodiodes are a photoelectric converter, the output voltage of the photodiodes is proportional to the input purple LED illumination, the digital voltage value of the output of the photodiodes which do not receive the purple LED illumination is approximately 0V, and the output voltage of the photodiodes which receive the purple LED illumination (the purple LED light passes through the micro-droplets) is between 0.5 and 2V, so that the photoelectric conversion can be completed; the processor 42 is electrically connected to the droplet positioning detection device 41, and the processor 42 is configured to receive the electrical signal sent by the photodiode array, as shown in fig. 3, and determine the position of the droplet according to the position of the photodiode in the photodiode array that sends the electrical signal.
The relationship between the contact angle between the plate-under-chip hydrophobic layer 13 and the droplet 20 and the dielectric layer voltage drop can be expressed by the Young-Lippmann equation:
wherein,indicating the initial contact angle of the droplet 20 with the hydrophobic layer when no voltage is applied,represents the surface tension between the lyophobic droplet and the gas, c represents the total capacitance value, and V represents the voltage drop of the dielectric layer.
As shown in fig. 3, when alternating voltages with certain amplitude and frequency are applied to the upper and lower ITO conductive glass 10, since there is no illumination, the impedance value of the photoconductive layer 11 is very large, the voltage drop falls on the photoconductive layer instead of the dielectric layer, and as known from Young-Lippmann equation, the voltage is applied at this time, so that the droplet does not move; when the purple LED light source emits light, the impedance value of the light guide layer is reduced to be very small under the condition of illumination, the voltage drop at the moment can fall on the medium layer, the contact angle of the liquid drop can be reduced according to the Young-Lippmann equation, the curvature radiuses of the two sides of the liquid drop are inconsistent at the moment, the pressure difference is generated inside the liquid drop, and when the driving force in the liquid drop is larger than the resistance of the liquid drop, the liquid drop can move in the light spot irradiation direction. Thus, the slowly moving spot will achieve control and manipulation of the droplet.
As shown in fig. 3, the black silicone oil 21 in the chip has a strong light absorption capacity, so that besides the violet LED light passing through the liquid drop, the rest of the violet LED light can be absorbed by the black silicone oil, that is, only the photodiode corresponding to the position of the liquid drop can receive the violet LED light signal, and the photodiodes at other positions can not receive the violet LED light signal, so that the device can realize accurate positioning of the liquid drop.
The utility model is not a matter of the known technology.
The above embodiments are provided to illustrate the technical concept and features of the present utility model and are intended to enable those skilled in the art to understand the content of the present utility model and implement the same, and are not intended to limit the scope of the present utility model. All equivalent changes or modifications made in accordance with the spirit of the present utility model should be construed to be included in the scope of the present utility model.
Claims (4)
1. A droplet registration detection apparatus for a photo-induced dielectric wetting chip, comprising:
the structure of the photoinduced dielectric wetting chip (1) can be divided into an upper part and a lower part, and the lower half part of the structure of the photoinduced dielectric wetting chip is sequentially from bottom to top: the light-induced dielectric wetting chip comprises ITO conductive glass (10), a light guide layer (11), a dielectric layer (12) and a hydrophobic layer (13), wherein the upper half part of the structure of the light-induced dielectric wetting chip is sequentially provided with the hydrophobic layer (13) and the ITO conductive glass (10) from bottom to top; the upper part and the lower part of the structure of the light-induced dielectric wetting chip are connected by a double-sided tape (14), so that a cavity for droplet operation is formed;
an ITO conductive glass (10), wherein the ITO conductive glass at the upper part of the photoinduced dielectric wetting chip structure and the ITO conductive glass at the lower part of the photoinduced dielectric wetting chip structure can be applied with alternating current with a certain amplitude and frequency to charge the photoinduced dielectric wetting chip;
a liquid (2) within the chamber for droplet operations, sandwiched between a hydrophobic layer of an upper portion of the photo-induced dielectric wetting chip structure and a hydrophobic layer of a lower portion of the photo-induced dielectric wetting chip structure, comprising droplets (20) and black silicone oil (21);
the liquid drop positioning detection device (4) is positioned right above the photoinduced dielectric wetting chip and consists of a photodiode array (41) and a processor (42), and the size of the photodiode array is consistent with the length and the width of the chip;
and the purple LED (3) is used for generating light spots to drive the liquid drops, and enabling light transmitted through the liquid drops to be received by the liquid drop positioning detection device so as to realize liquid drop positioning.
2. The drop placement detection device according to claim 1, wherein the photodiode array is configured to receive light emitted from the violet LED that is transmitted through the drop and to perform photoelectric conversion;
the processor is electrically connected with the photodiode array, and is used for receiving the electric signals sent by the photodiode array and judging the positions of the liquid drops according to the positions of the photodiodes (410) of the optical signals received by the photodiode array.
3. The photo-induced dielectric wetting chip droplet positioning detection device according to claim 1, characterized in that the chamber for droplet manipulation is filled with black silicone oil surrounding the droplet such that light emitted by the violet LED can be received by the photodiode array only through a portion of the droplet.
4. The device for positioning and detecting the liquid drop of the photoinduced dielectric wetting chip according to claim 1, wherein after alternating current with a certain amplitude and frequency is supplied to the upper and lower ITO conductive glass parts of the structure of the photoinduced dielectric wetting chip, a light spot generated by the purple LED on the chip changes the conductive characteristic of the photoconductive layer, and when the light spot irradiates one side of the liquid drop, a dielectric electrowetting effect occurs on the one side, so that the contact angle between the edge of the liquid drop on the irradiated side and a hydrophobic layer is reduced, and a pressure difference is generated inside the liquid drop, thereby moving the liquid drop towards the irradiation direction of the purple LED.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321777153.8U CN220460714U (en) | 2023-07-06 | 2023-07-06 | Liquid drop positioning detection device of photoinduction dielectric wetting chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321777153.8U CN220460714U (en) | 2023-07-06 | 2023-07-06 | Liquid drop positioning detection device of photoinduction dielectric wetting chip |
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| Publication Number | Publication Date |
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| CN220460714U true CN220460714U (en) | 2024-02-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202321777153.8U Active CN220460714U (en) | 2023-07-06 | 2023-07-06 | Liquid drop positioning detection device of photoinduction dielectric wetting chip |
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| Country | Link |
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| CN (1) | CN220460714U (en) |
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2023
- 2023-07-06 CN CN202321777153.8U patent/CN220460714U/en active Active
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