CN220425389U - Centrifuge tube pipetting device - Google Patents
Centrifuge tube pipetting device Download PDFInfo
- Publication number
- CN220425389U CN220425389U CN202321841445.3U CN202321841445U CN220425389U CN 220425389 U CN220425389 U CN 220425389U CN 202321841445 U CN202321841445 U CN 202321841445U CN 220425389 U CN220425389 U CN 220425389U
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- centrifuge tube
- pipette
- tube
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- 239000007788 liquid Substances 0.000 claims abstract description 77
- 230000007246 mechanism Effects 0.000 claims abstract description 15
- 230000000149 penetrating effect Effects 0.000 claims abstract description 4
- 239000012780 transparent material Substances 0.000 claims description 6
- 238000005213 imbibition Methods 0.000 abstract 3
- 238000000926 separation method Methods 0.000 description 9
- 239000012530 fluid Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 206010044565 Tremor Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000032798 delamination Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
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- Centrifugal Separators (AREA)
Abstract
The utility model discloses a centrifuge tube pipetting device, which comprises a centrifuge tube, a pipette and a positioning mechanism; the centrifugal tube is internally provided with a tube cavity with an opening at the top, the tube cavity is used for containing separated liquid after centrifugal layering, and the side wall of the centrifugal tube is at least provided with a vertical transparent observation area; the pipette is movably inserted into the lumen of the centrifuge tube, a cavity is arranged in the pipette, the top of the cavity is opened, the bottom area of the cavity is a pipetting cavity, the bottom surface of the pipetting cavity is an inclined surface, and a pipetting notch is arranged on the side surface of the top of the pipetting cavity and communicated with the outside; the positioning mechanism is used for positioning the position of the pipette penetrating into the centrifuge tube. The liquid that sets up the pipetting chamber and be different layers is located different cavities respectively, just can not mutual interference when adopting the imbibition of imbibition equipment, can not inhale the liquid of next floor, also can not make the liquid of lower floor take place to mix with the liquid in the imbibition, makes things convenient for the collection of layering liquid greatly, guarantees the reliability of collection sample.
Description
Technical Field
The utility model relates to the technical field of liquid separation, in particular to a centrifuge tube pipetting device.
Background
Centrifugal separation of biomolecules is the most commonly used biochemical separation method, because different biomolecules have different volumes and densities and can be separated by sedimentation under the action of different centrifugal forces.
Centrifugal separation of cell fluids is a common experimental method, which can separate different components in the cell fluids, so that the composition and functions of the cell fluids can be better studied. In the centrifugal layering process, cell fluid is placed in a centrifuge tube, and then centrifuged by a centrifuge rotating at a high speed, so that layering of different layers is finally formed. When treating cell separation liquid, the liquid level is divided into multiple layers after centrifugation, and a liquid-transferring gun, a separating funnel or a syringe is generally adopted to extend into a centrifugal tube for liquid suction. During normal liquid suction, the centrifuge tube is required to be held to be at the same height as the sight line, so that liquid suction is convenient, but the centrifuge tube is easy to shake, so that layered liquid is shake and even remixed. And when each layer of liquid is absorbed respectively, the next layer of liquid is absorbed easily due to hand shake, and the cell collection effect is affected.
Disclosure of Invention
The utility model aims to provide a centrifuge tube pipetting device which has the advantages of avoiding layered mixing and facilitating pipetting separation.
In order to achieve the above object, the solution of the present utility model is:
a centrifuge tube pipetting device comprises a centrifuge tube, a pipette and a positioning mechanism;
the centrifugal tube is internally provided with a tube cavity with an opening at the top, the tube cavity is used for containing separated liquid after centrifugal layering, and the side wall of the centrifugal tube is at least provided with a vertical transparent observation area;
the pipette is movably inserted into the lumen of the centrifuge tube, a cavity is arranged in the pipette, the top of the cavity is opened, the bottom area of the cavity is a pipetting cavity, the bottom surface of the pipetting cavity is an inclined surface, and a pipetting notch is arranged on the side surface of the top of the pipetting cavity and communicated with the outside;
the positioning mechanism is used for positioning the position of the pipette penetrating into the centrifuge tube.
Further, the bottom surface of the pipette is the same inclined surface as the bottom surface of the pipetting chamber.
Further, at least the bottom pipetting cavity of the pipette is made of transparent materials.
Furthermore, the centrifuge tube and the whole pipette are made of transparent materials.
Further, the positioning mechanism comprises an internal thread arranged on the inner wall of the centrifugal tube and an external thread arranged on the outer wall of the pipette, and the internal thread and the external thread are in threaded fit with each other.
Further, the internal threads are at least distributed at the middle lower part of the cylindrical section of the centrifugal tube, and the number of turns of the external threads is smaller than that of the internal threads.
Further, the internal thread is of a circumferentially segmented structure, the internal thread comprises two circumferentially spaced sub internal thread segments, two unthreaded areas are arranged between the circumferentially spaced sub internal thread segments, and at least one unthreaded area is the transparent observation area.
Further, the thread is located at the upper position of the pipetting cavity, the lower edge of the external thread is aligned with the lower edge of the liquid inlet notch, and the external thread is subjected to abdication treatment at the liquid inlet notch.
Further, the liquid inlet notch extends upwards to be communicated with the top opening of the cavity.
After the technical scheme is adopted, the bottom of the pipette can be penetrated into the lumen of the centrifuge tube from top to bottom, and the positioning mechanism is utilized for positioning and fixing. The liquid of last layer flows into the pipetting chamber by utilizing the liquid inlet notch, is spatially separated from the liquid of the next layer, even if different layers of liquid are respectively positioned in different chambers, the liquid can not interfere with each other when the liquid is sucked by adopting liquid sucking equipment (a pipetting gun, a separating funnel or an injector and the like) to extend into the pipetting chamber from the chamber, the liquid of the next layer can not be sucked, the liquid of the next layer can not be mixed with the liquid in the liquid suction, the collection of layered liquid is greatly facilitated, and the reliability of a collected sample is ensured.
The technical scheme can also have the following effects:
1. the transverse liquid level of the centrifuge tube is larger, so that the cross section of the pipetting cavity with the inclined bottom surface can be reduced, and the collection of layered liquid is facilitated.
2. By adopting the positioning mechanism, the pipette can be positioned to the required liquid level at one time, frequent shaking of the centrifuge tube can be avoided, and the hands are relaxed and not stiff.
Drawings
Fig. 1 is a perspective view of embodiment 1 of the present utility model;
FIG. 2 is a use state diagram of embodiment 1 of the present utility model;
FIG. 3 is a top view of FIG. 2;
FIG. 4 is a perspective cross-sectional view of FIG. 2;
FIG. 5 is a plan cross-sectional view of FIG. 2;
fig. 6 is a perspective view of embodiment 2 of the present utility model.
Description of the reference numerals: centrifuge tube 1, lumen 11, observation area 12, pipette 2, cavity 21, pipetting cavity 22, bottom surface 221 of pipetting cavity, liquid inlet notch 222, bottom surface 23 of pipette, positioning mechanism 3, internal thread 31, sub internal thread section 311, unthreaded area 312, external thread 32, cell separation liquid 4, first layer liquid 41, second layer liquid 42.
Detailed Description
For the purposes of making the objects, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, which are generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
In the description of the embodiments of the present application, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship conventionally put in place when the product of the application is used, or the orientation or positional relationship conventionally understood by those skilled in the art, is merely for convenience of describing the present application and simplifying the description, and is not indicative or implying that the apparatus or element referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.
Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more of the described features. In the description of the present application, the meaning of "a plurality" and "a number" is two or more, unless explicitly defined otherwise.
In the description of the embodiments of the present application, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically connected, electrically connected or can be communicated with each other; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art in a specific context.
In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.
The following disclosure provides many different embodiments or examples for implementing different structures of the present application. In order to simplify the disclosure of the present application, the components and arrangements of specific examples are described below. Of course, they are merely examples and are not intended to limit the present application. Furthermore, the present application may repeat reference numerals and/or letters in the various examples, which are for the purpose of brevity and clarity, and which do not in themselves indicate the relationship between the various embodiments and/or arrangements discussed.
In addition, the present application provides examples of various specific processes and materials, but one of ordinary skill in the art may recognize the application of other processes and/or the use of other materials.
Example 1
As shown in fig. 1 to 5, a centrifuge tube pipetting device of the utility model comprises a centrifuge tube 1, a pipette 2 and a positioning mechanism 3.
The centrifuge tube 1 is provided with a tube cavity 11 with an open top, and the tube cavity 11 is used for containing the cell separation liquid 4 or other separation liquids after centrifugation and delamination.
The pipette 2 may be an elongated cylindrical tube having an outer diameter slightly smaller than the inner diameter of the centrifuge tube 1, and may penetrate into the lumen 11 of the centrifuge tube 1 to separate the layered liquid.
Specifically, a cavity 21 is disposed in the pipette 2, a top opening of the cavity 21 is formed in the cavity 21, a bottom area of the cavity 21 is a pipetting cavity 22, a bottom surface 221 of the pipetting cavity is an inclined surface, a liquid inlet notch 222 is disposed on a top side surface of the pipetting cavity 22 and is communicated with the outside, and the liquid inlet notch 222 can extend upwards and be communicated with the top opening of the cavity 21.
Thus, the bottom of the pipette 2 can be inserted into the lumen 11 of the centrifuge tube 1 from top to bottom, when the liquid inlet notch 222 is aligned with the first layer of liquid 41 and is located above the dividing line between the first layer of liquid 41 and the second layer of liquid 42, the first layer of liquid 41 flows into the pipetting chamber 22 from the liquid inlet notch 222, so that the layered first layer of liquid 41 and the layered second layer of liquid 42 are respectively located in different chambers, and when a pipetting device (not shown in the drawings) is adopted, the pipetting device (which can be a pipetting gun, a separating funnel or a syringe, etc.) stretches into the pipetting chamber 22 from the chamber 21 to perform pipetting, the mutual interference can not occur, the next layer of liquid can not be sucked, and the liquid in the lower layer can not be mixed with the liquid in pipetting.
Meanwhile, the bottom surface 221 of the pipetting cavity is an inclined surface, so that the horizontal cross-sectional area of the pipetting cavity 22 is reduced from top to bottom, liquid can be effectively collected, and layered liquid collection is facilitated. After the first layer of liquid 41 is collected, the liquid enters the pipette 2 downwards, so that the liquid inlet notch 222 can align the liquid of each layer from top to bottom in sequence, and collect the liquid of each layer respectively.
In this embodiment, the bottom surface 23 of the pipette is inclined in the same manner as the bottom surface 221 of the pipetting chamber, so that the bottom side of the pipette 2 is formed as a tip portion, facilitating the insertion of the bottom of the pipette 2 into the liquid.
At least one vertical transparent observation area 12 is arranged on the side wall of the centrifugal tube 1, and at least the bottom pipetting cavity 22 of the pipette 2 is made of transparent materials, so that the position of the liquid inlet notch 222 of the pipette 2 penetrating into the centrifugal tube 1 can be observed conveniently. Of course, the centrifuge tube 1 and the pipette 2 may be entirely made of transparent materials.
The positioning mechanism 3 is used for positioning the pipette 2 deep into the centrifuge tube 1, and can control the position of the liquid inlet notch 222 of the pipette 2 to keep the liquid in a certain layer, so that the liquid entering the pipette 2 is ensured to be liquid in the same layer, and the interference caused by shaking of hands of people is reduced.
In this embodiment, the positioning mechanism 3 includes an internal thread 31 provided on the inner wall of the centrifuge tube 1, and an external thread 32 provided on the outer wall of the pipette 2, where the internal thread 31 and the external thread 32 are in threaded engagement with each other; the internal thread 31 is at least distributed at the middle lower part of the cylindrical section of the centrifuge tube 1, and the number of turns of the external thread 32 can be less than that of the internal thread 31, so that the pipette 2 can quickly and laborsaving go deep into the centrifuge tube 1.
The internal thread 31 may be a complete thread structure or a circumferentially segmented structure. The internal thread 31 of this embodiment is a circumferentially segmented structure, and includes two circumferentially spaced sub-internal thread segments 311, two unthreaded regions 312 are disposed between the two circumferentially spaced sub-internal thread segments 311, and at least one unthreaded region 312 may be the transparent observation region 12 described above, so as to facilitate observation of the position of the pipette 2.
The external thread 32 may be located above the pipetting chamber 22, the lower edge of the external thread 32 may be aligned with the lower edge of the liquid inlet notch 222, so that the position of the liquid inlet notch 222 is convenient to be observed in the rotation process, and the external thread 32 is abducted at the liquid inlet notch 222.
Therefore, by utilizing the threaded fit of the internal threads 31 and the external threads 32 of the positioning mechanism 3, the rotary pipette 2 gradually goes deep into the centrifugal tube 1, and the relative positioning and fixing of the positions of the two are kept, so that the stable collection of layered liquid is facilitated.
Example 2
As shown in fig. 6, the structure of this embodiment is basically the same as that of embodiment 1 described above, and the main difference is that the liquid inlet opening of this embodiment is not connected to the top opening of the cavity 21 upwards, but is a notch formed in the lower portion of the pipette 2 and the top of the pipetting cavity 22, so that the purpose of liquid inlet can be achieved.
The above description is only a preferred embodiment of the present utility model, and the protection scope of the present utility model is not limited to the above examples, and all technical solutions belonging to the concept of the present utility model belong to the protection scope of the present utility model. It should be noted that equivalent changes and modifications can be made by those skilled in the art without departing from the principles of the present utility model, which still falls within the scope of the present utility model.
Claims (9)
1. A centrifuge tube pipetting device, characterized in that:
comprises a centrifuge tube, a pipette and a positioning mechanism;
the centrifugal tube is internally provided with a tube cavity with an opening at the top, the tube cavity is used for containing separated liquid after centrifugal layering, and the side wall of the centrifugal tube is at least provided with a vertical transparent observation area;
the pipette is movably inserted into the lumen of the centrifuge tube, a cavity is arranged in the pipette, the top of the cavity is opened, the bottom area of the cavity is a pipetting cavity, the bottom surface of the pipetting cavity is an inclined surface, and a pipetting notch is arranged on the side surface of the top of the pipetting cavity and communicated with the outside;
the positioning mechanism is used for positioning the position of the pipette penetrating into the centrifuge tube.
2. The centrifuge tube pipetting device of claim 1 wherein: the bottom surface of the pipette is the same inclined plane as the bottom surface of the pipetting cavity.
3. The centrifuge tube pipetting device of claim 1 wherein: at least the bottom pipetting cavity of the pipette is made of transparent materials.
4. The centrifuge tube pipetting device of claim 1 wherein: the centrifuge tube and the pipette are made of transparent materials.
5. The centrifuge tube pipetting device of claim 1 wherein: the positioning mechanism comprises an internal thread arranged on the inner wall of the centrifugal tube and an external thread arranged on the outer wall of the pipette, and the internal thread and the external thread are in threaded fit with each other.
6. The centrifuge tube pipetting device of claim 5 wherein: the internal threads are at least distributed at the middle lower part of the cylindrical section of the centrifugal tube, and the number of turns of the external threads is smaller than that of the internal threads.
7. The centrifuge tube pipetting device of claim 5 wherein: the internal thread is of a circumferentially segmented structure, the internal thread comprises two circumferentially separated sub internal thread segments, two unthreaded areas are arranged between the two circumferentially spaced sub internal thread segments, and at least one unthreaded area is the transparent observation area.
8. The centrifuge tube pipetting device of claim 5 wherein: the thread is located at the upper position of the pipetting cavity, the lower edge of the external thread is aligned with the lower edge of the liquid inlet notch, and the external thread is subjected to abdication treatment at the liquid inlet notch.
9. The centrifuge tube pipetting device of claim 1 wherein: the liquid inlet notch extends upwards to be communicated with the top opening of the cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321841445.3U CN220425389U (en) | 2023-07-13 | 2023-07-13 | Centrifuge tube pipetting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321841445.3U CN220425389U (en) | 2023-07-13 | 2023-07-13 | Centrifuge tube pipetting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220425389U true CN220425389U (en) | 2024-02-02 |
Family
ID=89698728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321841445.3U Active CN220425389U (en) | 2023-07-13 | 2023-07-13 | Centrifuge tube pipetting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220425389U (en) |
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2023
- 2023-07-13 CN CN202321841445.3U patent/CN220425389U/en active Active
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