CN220364523U - Sample reaction tank for FISH pretreatment equipment - Google Patents

Sample reaction tank for FISH pretreatment equipment Download PDF

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Publication number
CN220364523U
CN220364523U CN202321847603.6U CN202321847603U CN220364523U CN 220364523 U CN220364523 U CN 220364523U CN 202321847603 U CN202321847603 U CN 202321847603U CN 220364523 U CN220364523 U CN 220364523U
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Prior art keywords
groove
sample
reaction tank
sample reaction
tank
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CN202321847603.6U
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戢汇亮
肖永军
张忠雄
陈传坤
魏亮
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Wuhan Youzhiyou Medical Technology Co ltd
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Wuhan Youzhiyou Medical Technology Co ltd
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Abstract

The utility model relates to the technical field of medical in-vitro diagnostic instruments, in particular to a sample reaction tank for FISH pretreatment equipment, which comprises a cover body device and a main body device; the cover body device comprises a groove cover and a sample bracket, and the lower end of the groove cover is connected with the sample bracket; the sample is placed on the sample support, and the groove cover is grabbed and placed in the accommodating cavity, so that experimental differences caused by directly grabbing the sample and placing the sample are avoided; the main body device comprises a containing groove, a containing cavity is formed in the containing groove, the sample support is placed in the containing cavity, a stirring piece is further arranged in the containing cavity, and the stirring piece is detachably arranged in the containing cavity and can rotate in the containing cavity; the stirring piece is used for stirring, so that the flow efficiency of the reagent can be effectively improved and controlled, and the efficiency of cleaning the reaction tank can be improved after detection is completed.

Description

Sample reaction tank for FISH pretreatment equipment
Technical Field
The utility model relates to the technical field of medical in-vitro diagnostic instruments, in particular to a sample reaction tank for FISH pretreatment equipment.
Background
Conventional paraffin-embedded tissue sections (conventional sections) are the most widely used method in conventional slice making technology of histology, are commonly used for researching, observing and judging morphological changes of cell tissues in pathology, can be used for qualitative and positioning observation research of macromolecular substances such as polypeptides, proteins and the like of the cell tissues in the tissue sections in combination with immunological technology, can be used for gene positioning and state analysis in cells in combination with a FISH (fluorescence in situ hybridization technology) method, and are widely used in research in the field of multidisciplinary at present.
In conventional FISH testing, paraffin-embedded tissue sections are subjected to dewaxing, penetrating agent penetration, pepsin digestion, washing, draining, and the like. However, the lack of standardized operation can result in errors in the detection assay due to varying levels of personnel operation. In the prior art, the method for detecting the flow of the reagent in the reaction tank generally achieves the purpose of the flow of the reagent in the reaction tank by shaking the whole reaction tank or blowing air at the bottom of the reaction tank, and the method can lead to low flow efficiency of the reagent detected in the reaction tank, reduce the reaction efficiency of the reagent and the cleaning effect after the reaction is finished.
Disclosure of Invention
The sample reaction tank for the FISH pretreatment equipment provided by the utility model can be used for solving the technical problems that in the prior art, the FISH detection cannot be unified with standard operation standards, so that experimental difference exists in detection, the flow efficiency of detection reagent in the sample reaction tank is low, the reaction efficiency is low and the cleaning effect is poor after the reaction is finished.
(II) technical scheme
In order to solve the above technical problems, an embodiment of the present utility model provides a sample reaction tank for FISH pretreatment apparatus, including a cover device and a main body device;
the cover body device comprises a groove cover and a sample bracket, and the lower end of the groove cover is connected with the sample bracket;
the main body device comprises a containing groove, a containing cavity is formed in the containing groove, the sample support is placed in the containing cavity, a stirring piece is further arranged in the containing cavity, and the stirring piece is detachably installed in the containing cavity and can rotate in the containing cavity.
Further, the main body device further comprises a heat dissipation mechanism, and the heat dissipation mechanism is connected with the containing groove.
Further, the heat dissipation mechanism comprises a radiator and a refrigerating sheet, the radiator is connected with the refrigerating sheet, and the radiator is embedded into the outer wall of the accommodating groove.
Further, the radiator comprises a radiating plate and radiating protrusions, one side of the radiating plate is connected with the refrigerating plate, the radiating protrusions are arranged on the other side of the radiating plate, through grooves are formed in the outer wall of the accommodating groove, and the radiating protrusions are embedded into the through grooves.
Further, the heat dissipation mechanism is provided with two groups, which are respectively positioned at two opposite sides of the accommodating groove.
Further, the main body device further comprises a driving mechanism, and the driving mechanism is used for driving the stirring piece to rotate in the accommodating cavity.
Further, the driving mechanism comprises a magnetic component and a driving motor;
the magnetic component comprises a magnet and a ferromagnetic element, and the driving motor and the magnet are arranged outside the accommodating groove and are positioned at the bottom of the accommodating groove;
the stirring piece is provided with a ferromagnetic element or made of ferromagnetic materials, and the driving motor is used for driving the magnet to rotate so as to drive the stirring piece to rotate.
Further, the side wall of the accommodating groove is also provided with two communicating ports for communicating the accommodating cavity, the two communicating ports are communicated through a communicating pipe, and a photoelectric sensor is arranged on the communicating pipe.
Further, one side of the accommodating groove is provided with an overflow port communicated with the accommodating cavity.
Further, a temperature probe is further arranged in the accommodating groove and used for monitoring the temperature in the accommodating groove.
The utility model has the beneficial effects that: the utility model provides a sample reaction tank for a FISH pretreatment device, which comprises a cover body device and a main body device; the cover body device comprises a groove cover and a sample bracket, and the lower end of the groove cover is connected with the sample bracket; the sample is placed on the sample support, and the groove cover is grabbed and placed in the accommodating cavity, so that experimental differences caused by directly grabbing the sample and placing the sample are avoided; the main body device comprises a containing groove, a containing cavity is formed in the containing groove, the sample support is placed in the containing cavity, a stirring piece is further arranged in the containing cavity, and the stirring piece is detachably arranged in the containing cavity and can rotate in the containing cavity; the stirring piece is used for stirring, so that the flow efficiency of the reagent can be effectively improved and controlled, and the efficiency of cleaning the reaction tank can be improved after detection is completed.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a sample reaction tank of a FISH pretreatment apparatus according to an embodiment of the present utility model;
fig. 2 is a schematic diagram of a sample reaction tank of FISH pretreatment apparatus according to an embodiment of the present utility model.
Icon: 1-a cover device; 101-a trough cover; 102-sample holder; 103-a heat dissipation mechanism; 1031-a heat sink; 10311-a heat dissipation plate; 10312—heat dissipating bump; 1032-refrigerating sheets; 104-a driving mechanism; 1041-a magnet; 1042-driving motor;
2-a body device; 201-a receiving groove; 2011-a communication port; 2012-communicating pipe; 2013-a photosensor; 2014-overflow port; 202-stirring piece.
Detailed Description
The technical solutions of the present utility model will be clearly and completely described in connection with the embodiments, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be noted that the azimuth or positional relationship indicated by the terms "upper", "lower", etc. are based on the azimuth or positional relationship shown in the drawings, and are merely for convenience of describing the present utility model and simplifying the description, and are not indicative or implying that the apparatus or element in question must have a specific azimuth, be constructed and operated in a specific azimuth, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; the communication may be direct or indirect through an intermediate medium, or may be internal to two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art. Furthermore, in the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.
As shown in fig. 1 and 2, the present utility model provides a sample reaction tank for FISH pretreatment apparatus, comprising a cover device 1 and a main body device 2; the cover body device 1 comprises a groove cover 101 and a sample bracket 102, wherein the lower end of the groove cover 101 is connected with the sample bracket 102; the main body device 2 comprises a holding groove 201, a holding cavity is formed in the holding groove 201, the sample support 102 is placed in the holding cavity, a stirring piece 202 is further arranged in the holding cavity, and the stirring piece 202 is detachably arranged in the holding cavity and can rotate in the holding cavity.
In the present embodiment, the sample reaction tank includes a cover device 1 and a main body device 2; the cover body device 1 comprises a groove cover 101 and a sample bracket 102, wherein the lower end of the groove cover 101 is connected with the sample bracket 102; the sample is placed on the sample support 102, and the sample is placed in the accommodating cavity through the grabbing groove cover 101, so that experimental differences caused by directly grabbing the sample for placement are avoided; the main body device 2 comprises a containing groove 201, a containing cavity is formed in the containing groove 201, the sample support 102 is placed in the containing cavity, a stirring piece 202 is further arranged in the containing cavity, and the stirring piece 202 is detachably arranged in the containing cavity and can rotate in the containing cavity; the stirring means 202 can effectively improve and control the flow efficiency of the reagent, and can also improve the efficiency of cleaning the reaction tank after the completion of the detection.
In this embodiment, the liquid in the accommodating tank 201 is allowed to flow by stirring the sample reaction process using the stirring member, so that the reaction is more efficient and thorough, and the cleaning step is more effective.
According to an embodiment of the present utility model, as shown in fig. 2, the main body device 2 further includes a heat dissipation mechanism 103, where the heat dissipation mechanism 103 is connected to the accommodating groove 201.
Further, the heat dissipation mechanism 103 includes a heat sink 1031 and a refrigerating sheet 1032, the heat sink 1031 is connected to the refrigerating sheet 1032, and the heat sink 1031 is embedded in the outer wall of the accommodation groove 201.
In the present embodiment, the main body device 2 is provided with the heat radiation mechanism 103, the heat radiation mechanism 103 is in contact with the accommodation groove 201, and the heat radiator 1031 transmits temperature into the accommodation groove 201.
The heat radiation mechanism 103 includes a heat radiator 1031 and a cooling fin 1032, the heat radiator 1031 is connected to the cooling fin 1032, and the heat radiator 1031 is embedded in the outer wall of the accommodation tank 201, and the heat radiator 1031 transfers the temperature of the cooling fin 1032 to the liquid in the accommodation tank 201.
Wherein, optionally, the radiator 1031 is connected with the temperature output surface of the refrigerating sheet 1032, and the radiator 1031 is embedded into the outer wall of the accommodating groove 201, so that the temperature produced by the refrigerating sheet 1032 can be transferred to the liquid in the accommodating groove 201 more effectively.
According to an embodiment of the present utility model, as shown in fig. 2, the heat sink 1031 includes a heat dissipation plate 10311 and a heat dissipation protrusion 10312, one side of the heat dissipation plate 10311 is connected with the refrigerating plate 1032, the other side of the heat dissipation plate 10311 is provided with the heat dissipation protrusion 10312, the outer wall of the accommodating groove 201 is provided with a through groove, and the heat dissipation protrusion 10312 is embedded into the through groove.
In this embodiment, one side of the heat spreader 1031 is connected to the refrigerating plate 1032 for transferring the temperature of the refrigerating plate 1032 to the liquid in the accommodating groove 201, the heat spreader 1031 is connected to the temperature output surface of the refrigerating plate 1032, a plurality of heat dissipating protrusions 10312 are disposed on the other side of the heat spreader 1031 away from the refrigerating plate 1032, the heat dissipating protrusions 10312 of the heat spreader 1031 are embedded into the through groove of the accommodating groove 201 and contact with the reagent in the accommodating cavity, and the heat dissipating protrusions 10312 are soaked by the reagent in the accommodating cavity, wherein the heat dissipating protrusions 10312 are preferably configured as columnar protrusions, which can increase the contact area between the heat spreader 1031 and the reagent in the accommodating cavity, thereby being beneficial to transferring the temperature of the output surface of the refrigerating plate 1032 to the reagent in the accommodating cavity more efficiently and reducing the time required for raising and lowering the temperature of the reagent.
In this embodiment, the heat dissipation surface of the heat sink 1031 is a columnar heat dissipation protrusion 10312, which can directly contact with the reagent in the accommodating cavity, and the columnar protrusion structure increases the contact area with the reagent, thereby accelerating the temperature transfer rate and improving the temperature transfer efficiency.
The heat dissipation protrusion 10312 of the heat dissipation device 1031 may be, but not limited to, a columnar protrusion structure, or other protrusion structures that help to increase the contact area between the heat dissipation surface of the heat dissipation device 1031 and the reagent in the accommodating cavity, which does not deviate from the design concept of the present utility model and is intended to fall within the scope of the present utility model.
According to one embodiment of the present utility model, as shown in fig. 2, the heat dissipation mechanism 103 is provided with two groups, which are respectively located at two opposite sides of the accommodating groove 201.
In this embodiment, preferably, the heat dissipation mechanism 103 is provided with two groups and is respectively located at two opposite sides of the accommodating groove 201, so that the contact area between the heat dissipation protrusion 10312 and the reagent in the accommodating cavity can be maximized, the temperature transfer can be more uniform, and the transfer efficiency of transferring the temperature from the refrigerating plate 1032 to the accommodating cavity is significantly improved.
According to an embodiment of the present utility model, as shown in fig. 2, the main body device 2 further includes a driving mechanism 104, where the driving mechanism 104 is used to drive the stirring member 202 to rotate in the accommodating cavity.
In this embodiment, the main body device 2 further comprises a driving mechanism 104, wherein the driving mechanism 104 is preferably located at the outer side of the bottom of the accommodating groove 201 and is used for driving the stirring member 202 to rotate in the accommodating cavity.
According to one embodiment of the present utility model, as shown in FIG. 2, the drive mechanism 104 includes a magnetic attraction assembly and a drive motor 1042; the magnetic attraction assembly comprises a magnet 1041 and a ferromagnetic element, and the driving motor 1042 and the magnet 1041 are arranged outside the accommodating groove 201 and are positioned at the bottom of the accommodating groove 201; the stirring member 202 is provided with a ferromagnetic element, or the stirring member 202 is made of ferromagnetic material, and the driving motor 1042 is used for driving the magnet 1041 to rotate so as to drive the stirring member 202 to rotate.
In this embodiment, the drive mechanism 104 includes a magnetic attraction assembly and a drive motor 1042; the magnetic attraction assembly comprises a magnet 1041 and a ferromagnetic element, and the driving motor 1042 and the magnet 1041 are arranged outside the accommodating groove 201 and are positioned at the bottom of the accommodating groove 201; the driving mechanism 104 can directly control the stirring piece 202 to rotate, and in the reaction process of the sample in the accommodating cavity, the driving mechanism 104 is used for driving the stirring piece 202 to stir so as to enable the reagent in the accommodating cavity to flow, so that the reaction is more effective and thorough, and the effect is better in the cleaning step.
In this embodiment, the driving mechanism 104 includes a magnetic component and a driving motor 1042, the magnetic component includes a magnet 1041 and a ferromagnetic element, the magnet 1041 and the stirring member 202 are correspondingly installed at the outer side of the bottom of the accommodating groove 201, the driving motor 1042 is located at the bottom of the driving mechanism 104, the driving motor 1042 drives the magnet 1041 to rotate, the magnet 1041 drives the stirring member 202 to rotate, thereby stirring the reagent in the accommodating cavity, the reagent flows, the magnetic stirring mode can be used to effectively improve and control the flowing efficiency of the reagent, the magnet 1041 and the stirring member 202 are not connected by a physical mode, the risk of reagent leakage is avoided, the reagent in the accommodating cavity flows by using the magnetic stirring mode, the reaction is more effective and thorough, and the effect in the cleaning step can be better.
Preferably, two magnets 1041 are selected, the magnets 1041 are installed above the driving motor 1042, and the two magnets 1041 are placed in parallel, so that the stirring member 202 can be driven by the magnets 1041 to perform rotary stirring more smoothly.
The stirring member 202 is provided with a ferromagnetic element, or the stirring member 202 is made of a ferromagnetic material, and by setting the stirring member 202 to be a ferromagnetic material, or by installing a ferromagnetic element corresponding to the magnet on the stirring member 202, the rotation efficiency of the magnet 1041 driving the stirring member 202 can be effectively improved.
In this embodiment, the manner of driving the stirring member 202 to rotate further includes directly connecting with the stirring member 202 through a driving motor to drive it to rotate, or driving it to rotate through a transmission assembly, such as a gear transmission and a transmission rod transmission, etc., which do not depart from the design concept of the present utility model and shall fall within the protection scope of the present utility model.
According to an embodiment of the present utility model, as shown in fig. 1 and 2, the side wall of the accommodating groove 201 is further provided with two communication ports 2011 connected to the accommodating cavity, the two communication ports 2011 are communicated through a communication pipe 2012, and the communication pipe 2012 is provided with a photoelectric sensor 2013.
In this embodiment, the side wall of the accommodating groove 201 is further provided with two communicating ports 2011 communicating with the accommodating cavity, and the two communicating ports 2011 are communicated with each other through one communicating pipe 2012.
In this embodiment, the communicating pipe 2012 mounted on the side wall of the accommodating tank 201 is provided with a photosensor 2013, and the photosensor 2013 on the communicating vessel feeds back whether the pumped reagent reaches a predetermined capacity, and when the reagent liquid level in the accommodating chamber reaches a predetermined height, the photosensor 2013 on the communicating vessel triggers, thereby stopping pumping the reagent.
The accommodating groove 201 provided in this embodiment uses the combination of the communicating pipe 2012 and the photoelectric sensor 2013 to determine the height of the reagent liquid level in the accommodating cavity, so as to realize the quantification of reagent pumping, and the liquid path principle is simple and reliable.
As shown in fig. 1 and 2, one side of the accommodation groove 201 is provided with an overflow port 2014 communicating with the accommodation chamber.
In this embodiment, an overflow port 2014 connected with the accommodating cavity is formed on one side of the accommodating groove 201, when the equipment fails, the reagent in the accommodating cavity flows out from the overflow port 2014 due to gravity, so that the risk of circuit burnout caused by reagent overflow is avoided, and the use safety of the accommodating groove 201 in this embodiment is improved. An overflow outlet 2014 communicated with the accommodating cavity is formed in one side of the accommodating groove 201, and when the liquid inlet fails, the reagent can be automatically discharged from the overflow outlet 2014 when the liquid level is too high, so that serious consequences caused by flowing to a circuit part are avoided.
Wherein, optionally, a thicker pipeline is connected at the overflow outlet 2014, when the reagent overflows from the accommodating cavity, the reagent in the accommodating cavity can automatically flow out from the overflow outlet 2014 to avoid the reagent flowing to the circuit part, thereby causing more serious consequences.
As shown in fig. 2, a temperature probe is further disposed inside the accommodating groove 201, and the temperature probe is used for monitoring the temperature inside the accommodating groove 201.
In this embodiment, the temperature probe is disposed inside the accommodating groove 201, and the temperature probe can effectively monitor the temperature in the accommodating groove 201 and feed back to the operator, so that the operator can accurately control the temperature in the accommodating groove 201.
The foregoing is only illustrative of the present utility model and is not to be construed as limiting thereof, but rather as various modifications, equivalent arrangements, improvements, etc., within the spirit and principles of the present utility model.

Claims (10)

1. A sample reaction tank for FISH pretreatment apparatus, characterized by comprising a cover device (1) and a main body device (2);
the cover body device (1) comprises a groove cover (101) and a sample bracket (102), wherein the lower end of the groove cover (101) is connected with the sample bracket (102);
the main body device (2) comprises a containing groove (201), a containing cavity is formed in the containing groove (201), the sample support (102) is placed in the containing cavity, a stirring piece (202) is further arranged in the containing cavity, and the stirring piece (202) is detachably installed in the containing cavity and can rotate in the containing cavity.
2. A sample reaction tank for FISH pretreatment apparatus according to claim 1, characterized in that said main body means (2) further comprises a heat radiation mechanism (103), said heat radiation mechanism (103) being connected to said holding tank (201).
3. A sample reaction tank for FISH pretreatment apparatus according to claim 2, wherein the heat radiation mechanism (103) comprises a heat radiator (1031) and a refrigerating plate (1032), the heat radiator (1031) is connected to the refrigerating plate (1032), and the heat radiator (1031) is embedded in the outer wall of the housing tank (201).
4. A sample reaction tank for FISH pretreatment apparatus according to claim 3, wherein said heat sink (1031) comprises a heat radiation plate (10311) and heat radiation protrusions (10312), one side of said heat radiation plate (10311) is connected to said refrigerating plate (1032), said heat radiation protrusion (10312) is provided on the other side of said heat radiation plate (10311), a through groove is provided on the outer wall of said housing groove (201), and said heat radiation protrusions (10312) are embedded in said through groove.
5. The sample reaction tank for FISH pretreatment apparatus according to claim 4, wherein said heat dissipating means (103) is provided with two sets, respectively located on opposite sides of said receiving tank (201).
6. A sample reaction tank for a FISH pretreatment apparatus according to claim 1, wherein the main body means (2) further comprises a driving mechanism (104), and the driving mechanism (104) is configured to drive the stirring member (202) to rotate in the accommodating chamber.
7. The sample reaction cell for FISH pretreatment apparatus of claim 6, wherein said drive mechanism (104) comprises a magnetic attraction assembly and a drive motor (1042);
the magnetic component comprises a magnet (1041) and a ferromagnetic element, and the driving motor (1042) and the magnet (1041) are arranged outside the accommodating groove (201) and are positioned at the bottom of the accommodating groove (201);
the stirring piece (202) is provided with a ferromagnetic element, or the stirring piece (202) is made of ferromagnetic materials, and the driving motor (1042) is used for driving the magnet (1041) to rotate so as to drive the stirring piece (202) to rotate.
8. The sample reaction tank for FISH pretreatment apparatus according to claim 1, wherein the side wall of the holding tank (201) is further provided with two communicating ports (2011) communicating with the holding chamber, the two communicating ports (2011) are communicated with each other through a communicating pipe (2012), and the communicating pipe (2012) is provided with a photoelectric sensor (2013).
9. The sample reaction tank for FISH pretreatment apparatus according to claim 8, wherein one side of the accommodation tank (201) is provided with an overflow port (2014) communicating with the accommodation chamber.
10. A sample reaction tank for FISH pretreatment apparatus according to claim 9, characterized in that a temperature probe is further provided inside the accommodation tank (201) for monitoring the temperature inside the accommodation tank (201).
CN202321847603.6U 2023-07-13 2023-07-13 Sample reaction tank for FISH pretreatment equipment Active CN220364523U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202321847603.6U CN220364523U (en) 2023-07-13 2023-07-13 Sample reaction tank for FISH pretreatment equipment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202321847603.6U CN220364523U (en) 2023-07-13 2023-07-13 Sample reaction tank for FISH pretreatment equipment

Publications (1)

Publication Number Publication Date
CN220364523U true CN220364523U (en) 2024-01-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202321847603.6U Active CN220364523U (en) 2023-07-13 2023-07-13 Sample reaction tank for FISH pretreatment equipment

Country Status (1)

Country Link
CN (1) CN220364523U (en)

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