CN220352099U - Lung cancer primary cell separation culture device - Google Patents

Abstract

The utility model discloses a lung cancer primary cell separation culture device, and belongs to the technical field of cell separation culture. The lung cancer primary cell separation and culture device comprises an incubator, wherein a culture cylinder is arranged in the incubator, a filter screen is arranged in the middle of the culture cylinder, and a first supporting ring is arranged in the incubator; according to the utility model, the culture cylinder is kept in a vertical state through the second supporting ring, the guide rod and the first supporting ring, the motor drives the culture cylinder to rotate, centrifugal operation is carried out, cells of tissue blocks in separating liquid are separated, the guide rod is moved downwards through loosening the hoops, the limit of the pair of culture cylinders is contacted with the supporting ring, the culture cylinder is overturned, the waste liquid on the upper layer in the culture cylinder is poured out, the culture cylinder is reset to the vertical state, and cell culture liquid is injected through the connecting pipeline, so that the cells are cultured.

Description

Lung cancer primary cell separation culture device

Technical Field

The utility model relates to the technical field of cell separation culture, in particular to a lung cancer primary cell separation culture device.

Background

The primary lung cancer cells are cancer cells obtained from a cancerous lung organ and cultured in vitro in a simulated body environment.

The lung cancer primary cell separation culture device with the publication number of CN211546563U comprises a first box body, a second box body is embedded in the center of the top of the first box body, a first shell body is placed at the bottom of an inner cavity of the second box body, the bottom of the first shell body penetrates through the second box body and extends to the inner cavity of the first box body, and a test tube is placed at the axis of the inner cavity of the first shell body.

The device controls the external temperature of the culture tube through water bath heating, so that primary lung cancer cells are cultured at a stable temperature, but the device only cultures the cells, the separation and culture of the cells are not completed in a set of equipment, the separation and culture of the cells are easily interfered by the external environment, and the pollution probability is high.

Disclosure of Invention

The utility model aims to solve the problems in the prior art and provides a lung cancer primary cell separation and culture device.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the utility model provides a lung cancer primary cell separation culture apparatus, includes the incubator, the inside of incubator is provided with the culture drum, culture drum mid-mounting has the filter screen, be provided with support ring one in the incubator, support ring one outer lane rotates and is connected with support ring two, and the culture drum can rotate for support ring two, fixedly connected with guide bar on the support ring two, and the guide bar runs through the top of incubator and with incubator sliding connection, be provided with the pivoted power unit of drive culture drum in the incubator.

Preferably, the power mechanism is a motor, the output rotating shaft of the motor is hinged with a base, the bottom of the culture cylinder is connected with the base through a bolt, and the base is in sliding connection with the inner ring of the first supporting ring.

Preferably, the guide rod is provided with a hoop, the hoop is contacted with the top of the incubator, the guide rod is fixedly connected with a second inclined rod, and the second inclined rod is close to the top of the incubator.

Preferably, the first diagonal rod is fixedly connected to the culture cylinder, the second support ring is provided with a support column, the first diagonal rod is close to the support column, the support column penetrates through the second support ring and is in sliding connection with the second support ring, a spring is sleeved outside the support column, two ends of the spring are connected with the second support ring and the support column respectively, and a collecting frame is placed in the incubator.

Preferably, the incubator internally mounted has temperature sensor, incubator internally mounted has the division board, and the division board separates into the incubator inner chamber and cultivates the chamber and adjust the temperature the chamber, has placed the basin in the chamber that adjusts the temperature, install the fan on the incubator, the convulsions end of fan is located the cultivation intracavity, and the air-out end of fan is located the basin, sliding connection has the drawer on the chamber door of incubator, set up the vent in intercommunication cultivation chamber and the chamber that adjusts the temperature on the division board, the heating wire is installed to the intracavity that adjusts the temperature, and the heating wire is close to with the vent mutually.

Preferably, a fan is mounted on the partition plate, and the fan is close to the ventilation opening.

Compared with the prior art, the utility model provides a lung cancer primary cell separation and culture device, which has the following beneficial effects.

1. According to the utility model, the culture cylinder is kept in a vertical state through the second supporting ring, the guide rod and the first supporting ring, the motor drives the culture cylinder to rotate, centrifugal operation is carried out, cells of tissue blocks in separating liquid are separated, the guide rod is moved downwards through loosening the hoops, the limit of the pair of culture cylinders is contacted with the supporting ring, the culture cylinder is overturned, the waste liquid on the upper layer of the culture cylinder is poured out, the culture cylinder is reset to the vertical state, and cell culture liquid is injected through the connecting pipeline to culture cells.

2. According to the utility model, through the cooperation of the fan and the heating wire, when the temperature detected by the temperature sensor is not in the set temperature range, the fan extracts air in the culture cavity and sends the air into water in the water tank, then the air comes out of the water, is dried by the drying agent and is heated to a proper temperature by the heating wire, and enters the culture cavity through the ventilation opening, so that the internal culture temperature of the incubator is ensured.

Drawings

FIG. 1 is a plan view of the present utility model

FIG. 2 is a schematic view showing the internal structure of the incubator according to the present utility model

FIG. 3 is a schematic view showing the removal of waste liquid in a culture vessel according to the present utility model.

FIG. 4 is a schematic view showing the connection of the base and the culture cylinder.

In the figure:

1. an incubator; 2. a blower; 3. a drawer; 4. a guide rod; 5. heating wires; 6. a fan; 7. a partition plate; 8. a support column; 9. a collection frame; 10. a first supporting ring; 11. a filter screen; 12. a motor; 13. a temperature sensor; 14. a base; 15. a first inclined rod; 16. a second supporting ring; 17. a second inclined rod; 18. a culture cylinder.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

Referring to fig. 1-4, a lung cancer primary cell separation culture device comprises a culture box 1, wherein a box door is arranged on the front surface of the culture box 1, an observation window is formed in the box door, a sealing gasket can be arranged at a gap between the box door and the culture box 1, a culture cylinder 18 is arranged in the culture box 1, a connecting pipeline is arranged above the culture cylinder 18, separating liquid and cell culture liquid enter the culture cylinder 18 through the connecting pipeline, a filter screen 11 is arranged in the middle of the culture cylinder 18, the filter screen 11 is detachably connected to the middle of the culture cylinder 18 through bolts, and a large tissue block which is not separated by the separating liquid is blocked above the filter screen 11 by the filter screen 11.

The incubator 1 is internally provided with a power mechanism for driving the culture cylinder 18 to rotate, the power mechanism is a motor 12, an output rotating shaft of the motor 12 is hinged with a base 14, the bottom of the culture cylinder 18 is connected with the base 14 through a bolt, one end of the base 14 can be fixed with a limiting block through the output rotating shaft of the motor 12, the limiting block is contacted with a hinged seat arranged on the base 14, the base 14 can only be turned to one side far away from the limiting block, and the culture cylinder 18 can be turned relative to the output rotating shaft of the motor 12.

The incubator 1 is internally provided with a first support ring 10, a base 14 is in sliding connection with an inner ring of the first support ring 10, when the base 14 and the first support ring 10 are in sliding contact, the first support ring 10 limits the first support ring 14, so that the first support ring 10 faces upwards vertically, when the base 14 is separated from the first support ring 10, a culture cylinder 18 can be turned over, an outer ring of the first support ring 10 is rotationally connected with a second support ring 16, so that the culture cylinder 18 can rotate relative to the second support ring 16, a guide rod 4 is fixedly connected to the second support ring 16, the guide rod 4 penetrates through the top of the incubator 1 and is in sliding connection with the incubator 1, a sealing gasket can be arranged at the joint of the guide rod 4 and the incubator 1, sealing is guaranteed, and the guide rod 4 enables the second support ring 16 and the first support ring 10 to keep a horizontal state.

The guide rod 4 is provided with a hoop, the hoop is contacted with the top of the incubator 1, the bottom of the guide rod 4 can be locked at different positions in the incubator 1 by adjusting the tightness of the hoop, the culture cylinder 18 is kept in a vertical state through the support ring II 16, the guide rod 4 and the support ring I10, the motor 12 drives the culture cylinder 18 to rotate for centrifugal operation, cells of tissue blocks in separated liquid are separated, the guide rod 4 is fixedly connected with the inclined rod II 17, the inclined rod II 17 is close to the top of the culture cylinder 18, the inclined rod II 17 is arranged, when the inclined rod II 17 moves downwards, transverse thrust can be provided for the culture cylinder 18, the culture cylinder 18 after centrifugal operation is turned to the side opposite to the inclined rod II 17, and waste liquid on the upper layer of the culture cylinder 18 is poured out.

The first diagonal rod 15 is fixedly connected to the culture cylinder 18, the first diagonal rod 15 is arranged on the second support ring 16, the first diagonal rod 15 is close to the support column 8, the support column 8 penetrates through the first diagonal rod 15 and is in sliding connection with the second support ring 16, springs are sleeved outside the support column 8, two ends of each spring are connected with the second support ring 16 and the support column 8 respectively, the first diagonal rod 15 and the support column 8 are arranged to provide support for the overturned culture cylinder 18, the culture cylinder 18 is enabled to be overturned slowly, the first culture cylinder 18 is prevented from being overturned at one time, the springs are arranged, the culture cylinder 18 can be reset by the compressed springs during overturning, the first support ring 16 is always supported by the first diagonal rod 15 during overturning, after waste liquid is poured out, the second support ring 16 is upwards moved, the support column 8 is abutted to the first diagonal rod 15, the culture cylinder 18 is restored to a vertical state under the blocking condition, the first diagonal rod 15 is then in sliding contact with the base 14, the springs are compressed, cell culture liquid is injected into the culture cylinder 18 through a connecting pipeline, the cell culture cylinder 18 is prevented from being overturned, the cell culture cylinder 1 is placed in the cell culture cylinder 18, and the cell culture box 1 is placed in the cell culture cylinder is provided with the cell culture cylinder 9 through the cell culture cylinder, and the cell culture box is placed in the cell culture cylinder 9 is placed in the cell culture cylinder through the cell culture box 9, and the cell culture box is placed in the cell culture box is 9, and the cell culture container is placed in the cell culture container is in the cell culture 18.

The incubator 1 internally mounted has temperature sensor 13, temperature sensor 13 is used for detecting the inside temperature of incubator 1, the inside fixedly connected with division board 7 of incubator 1, and division board 7 separates into the incubator 1 inner chamber and adjusts the temperature the chamber, culture drum 18 is located the culture chamber, the basin has been placed to the intracavity that adjusts the temperature, water is full of in the basin, install fan 2 on the incubator 1, the air extraction end of fan 2 is located the culture chamber, and the air-out end of fan 2 is located the aquatic in the basin, sliding connection has drawer 3 on the chamber door of incubator 1, be provided with sealed pad between drawer 3 and the incubator 1, the drier has been placed on the drawer 3, and the micropore has been seted up on the drawer 3, the air that supplies drawer 3 below passes through and is dried by the drier on the drawer 3.

The vent that feeds through cultivates the chamber and adjusts the temperature chamber has been seted up on the division board 7, the heating wire 5 is installed to the temperature adjusting intracavity, the power or the voltage of accessible control heating wire 5 makes the heating temperature control of heating wire 5 be in the temperature of suitable cultivateing the cell, and heating wire 5 is close to with the vent mutually, cooperation through fan 2 and heating wire 5 sets up, when temperature sensor 13 detects the temperature and is not in the interval of settlement temperature, the air in the air extraction cultivate the chamber of fan 2 and send into the aquatic in the basin, then the air comes out from the aquatic, it is dry and by heating wire 5 to heat suitable temperature to pass through the drier, get into cultivate the intracavity through the vent, install fan 6 on the division board 7, and fan 6 is close to with the vent mutually, fan 6 will be followed the quick cultivation chamber that spreads out of air in the vent, guarantee the temperature in the incubator 1.

Working principle: separating liquid is injected into the culture cylinder 18 through a connecting pipeline, the large tissue blocks are blocked by the filter screen 11, the undigested tissue blocks are blocked above the filter screen 11 after standing for a certain period of time, the motor 12 drives the culture cylinder 18 to rotate, centrifugal operation is carried out, cells of the tissue blocks in the separating liquid are adhered to the bottom of the culture cylinder 18, and waste liquid is distributed on the upper layer.

After centrifugation, the guide rod 4 is pressed down to gradually separate the first support ring 10 from the base 14, meanwhile, the second support ring 16 moves down, and the compressed spring is reset during the period that the first support ring 10 and the culture cylinder 18 are not separated, so that the support column 8 always supports the first diagonal rod 15 during the period, after the first support ring 10 is separated from the base 14, the second diagonal rod 17 moves down to provide transverse thrust for the culture cylinder 18, so that the culture cylinder 18 after centrifugation is gradually overturned to the side opposite to the second diagonal rod 17 under the support of the support column 8 until the waste liquid on the upper layer of the culture cylinder 18 is poured out.

After the waste liquid is poured out, the guide rod 4 moves upwards, the support column 8 moves upwards and enables the culture cylinder 18 to restore to the vertical state through the inclined rod I15, the limiting block limits the base 14, the culture cylinder 18 is prevented from inclining to the opposite side of the inclined rod I15, meanwhile, the guide rod 4 continues to move upwards, the support ring I10 is in sliding contact with the base 14 and limits the base 14, the support column 8 slides downwards on the support ring II 16 under the blocking of the inclined rod I15, and the spring is compressed.

During cultivation, the culture medium is injected into the cultivation cylinder 18 through the connecting pipeline, the temperature is detected through the temperature sensor 13, when the temperature detected by the temperature sensor 13 is not in a set temperature range, the fan 2 extracts air of the cultivation cavity and sends the air into water in the water tank, then the air comes out of the water, the air is dried through the drying agent and is heated to a proper temperature by the heating wire 5, the air enters the cultivation cavity through the ventilation opening, the fan 6 rapidly distributes the air coming out of the ventilation opening into the cultivation cavity, the temperature in the cultivation box 1 is ensured, after the cultivation is finished, the box door can be opened, the limit of the cultivation cylinder 18 and the base 14 is relieved, and the cultivation cylinder 18 is taken down.

The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present utility model. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.

While embodiments of the present utility model have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the utility model, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the utility model.

Claims (6)

1. The utility model provides a lung cancer primary cell separation culture apparatus, includes incubator (1), its characterized in that, the inside of incubator (1) is provided with culture drum (18), culture drum (18) mid-mounting has filter screen (11), be provided with support ring one (10) in incubator (1), support ring one (10) outer lane rotates and is connected with support ring two (16), and culture drum (18) can rotate for support ring two (16), fixedly connected with guide bar (4) on support ring two (16), and guide bar (4) run through the top of incubator (1) and with incubator (1) sliding connection, be provided with the pivoted power unit of drive culture drum (18) in incubator (1).

2. The lung cancer primary cell separation and culture device according to claim 1, wherein the power mechanism is a motor (12), an output rotating shaft of the motor (12) is hinged with a base (14), the bottom of the culture cylinder (18) is connected with the base (14) through a bolt, and the base (14) is slidably connected with an inner ring of the first support ring (10).

3. The lung cancer primary cell separation and culture device according to claim 1, wherein a hoop is installed on the guide rod (4), the hoop is in contact with the top of the incubator (1), a second diagonal rod (17) is fixedly connected to the guide rod (4), and the second diagonal rod (17) is close to the top of the culture cylinder (18).

4. The lung cancer primary cell separation and culture device according to claim 1, wherein the culture cylinder (18) is fixedly connected with a first diagonal rod (15), a second support ring (16) is provided with a support column (8), the first diagonal rod (15) is close to the support column (8), the support column (8) penetrates through the second support ring (16) and is in sliding connection with the second support ring (16), a spring is sleeved outside the support column (8), two ends of the spring are respectively connected with the second support ring (16) and the support column (8), and a collecting frame (9) is placed in the incubator (1).

5. The lung cancer primary cell separation culture device according to claim 1, wherein a temperature sensor (13) is installed inside the culture box (1), a partition plate (7) is fixedly connected inside the culture box (1), an inner cavity of the culture box (1) is divided into a culture cavity and a temperature regulating cavity by the partition plate (7), a water tank is placed in the temperature regulating cavity, a fan (2) is installed on the culture box (1), an exhaust end of the fan (2) is located in the culture cavity, an air outlet end of the fan (2) is located in the water tank, a drawer (3) is connected to a box door of the culture box (1) in a sliding mode, a vent communicated with the culture cavity and the temperature regulating cavity is formed in the partition plate (7), an electric heating wire (5) is installed in the temperature regulating cavity, and the electric heating wire (5) is close to the vent.

6. The primary lung cancer cell separation and culture device according to claim 5, wherein a fan (6) is mounted on the partition plate (7), and the fan (6) is close to the ventilation opening.