CN220340031U - Super-resolution three-dimensional imaging device based on medium microspheres - Google Patents
Super-resolution three-dimensional imaging device based on medium microspheres Download PDFInfo
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- CN220340031U CN220340031U CN202322004107.0U CN202322004107U CN220340031U CN 220340031 U CN220340031 U CN 220340031U CN 202322004107 U CN202322004107 U CN 202322004107U CN 220340031 U CN220340031 U CN 220340031U
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Abstract
The utility model discloses a super-resolution three-dimensional imaging device based on medium microspheres, and relates to the technical field of optical imaging. The utility model mainly aims to solve the problem that the existing microscopic sample is difficult to observe the omnibearing shape when being observed, and provides the following technical scheme: the visual angle adjusting mechanism comprises a first visual angle adjusting mechanism and a second visual angle adjusting mechanism; the vision feedback module records the view acquired by the image acquisition module and transmits the view to the main control module; the image acquisition module comprises a rotating frame, a rotating shaft and an acquisition assembly. The beneficial effects are generated: the first visual angle adjusting mechanism can flexibly change the angle of the lens, the second visual angle adjusting mechanism controls the movement of the medium microsphere, and the first visual angle adjusting mechanism and the second visual angle adjusting mechanism are matched to realize the omnibearing scanning imaging of a sample, so that the imaging distortion problem caused by limited angle imaging is avoided.
Description
Technical Field
The utility model relates to the technical field of optical imaging, in particular to a super-resolution three-dimensional imaging device based on medium microspheres.
Background
Due to the existence of optical diffraction limits, the lateral resolution of conventional optical microscopes is far from meeting the resolution requirements of modern scientific techniques. Super-resolution imaging is a technology for improving the resolution of pictures, and achieves the effects of high precision and high definition by enabling the resolution of the pictures to exceed the traditional optical limit. Super-resolution imaging techniques are very widely used, for example in the fields of medicine, biology, material science, semiconductor physics, optical imaging, etc. A plurality of super-resolution microscopic imaging technologies have been sequentially proposed nowadays, and are closely focused by research teams at home and abroad. Among the optical super-resolution microscopic imaging technologies, the super-resolution imaging technology based on medium microspheres has the advantages of low cost, simple operation, high resolution and the like, and has attracted great attention. The super-resolution imaging can be realized by covering the surface of the sample with a film composed of microspheres and a transparent medium layer which are closely arranged and observing the surface by combining a traditional optical microscope.
The existing medium microsphere super-resolution imaging technology can observe some tiny structures, but can only realize imaging on a two-dimensional plane, and is not deep and comprehensive for observation of a sample, so that the imaging under multiple visual angles still has the following problems. Such as:
1) In observation of microscopic samples, the phenomenon that the shape of the sample is not easy to observe in all directions often exists.
2) When the medium microsphere and the film are fixed, and the micro structure is observed, the displacement and the angle of the lens cannot be accurately controlled due to the small height.
3) The aberration of the medium microsphere exists when the field of view of the objective lens deviates from the vertical angle, and the distortion phenomenon can occur when the imaging is observed at a limited angle.
Disclosure of Invention
The utility model aims to provide a super-resolution three-dimensional imaging device based on medium microspheres, which aims to solve the problem that the omni-directional shape is difficult to observe when the existing microscopic sample is observed.
The technical scheme for solving the technical problems is as follows:
a super-resolution three-dimensional imaging device based on medium microspheres, comprising: the system comprises a main control module, a visual feedback module, a visual angle adjusting mechanism and an image acquisition module, wherein the main control module is respectively in communication connection with the visual feedback module and the visual angle adjusting mechanism, and the visual angle adjusting mechanism comprises a first visual angle adjusting mechanism and a second visual angle adjusting mechanism; transmitting the view acquired by the image acquisition module to the main control module through the visual feedback module; the image acquisition module comprises a rotating frame, a rotating shaft and an acquisition assembly, the rotating frame is rotationally connected with the acquisition assembly through the rotating shaft, the first visual angle adjusting mechanism is connected with the rotating frame, and the imaging end of the visual feedback module is connected with the acquisition assembly; the second visual angle adjusting mechanism is rotatably connected with a carrying component for placing a sample, and the carrying component is positioned below the collecting component and corresponds to the collecting component.
The beneficial effects of adopting above-mentioned technical scheme are: the first visual angle adjusting mechanism can flexibly change the angle of the lens, the second visual angle adjusting mechanism can flexibly control the movement of the medium microsphere, and the first visual angle adjusting mechanism and the second visual angle adjusting mechanism are matched to realize the omnibearing scanning imaging of a sample, so that the imaging distortion problem caused by limited angle imaging is avoided.
Further, the carrier assembly comprises a medium microsphere, a pneumatic object stage and a curved surface supporting table, wherein the surface of the medium microsphere is packaged with a film, the pneumatic object stage is arranged on the upper surface of the curved surface supporting table, a sample is placed on the pneumatic object stage, and the medium microsphere film is positioned on the pneumatic object stage and covers the sample.
Further, the image acquisition module further comprises a mirror arm, and the mirror arm is connected with the rotating frame.
Further, the first visual angle adjusting mechanism and the second visual angle adjusting mechanism both comprise motors, the motors of the first visual angle adjusting mechanism are connected with the rotating frame through shaft sleeves, the motor output end of the second visual angle adjusting mechanism is connected with a driving gear in a matched mode, the end faces of the medium microspheres, the pneumatic objective table and the curved surface supporting table are respectively provided with a rotating shaft, and the end portions of the rotating shafts are provided with driven gears meshed with the driving gears.
Further, the shape of the medium microsphere thin film is bowl-shaped.
The utility model has the following beneficial effects:
1. the first visual angle adjusting mechanism can flexibly change the angle of the lens, and the lens barrel is controlled by the matching of the rotating frame, the rotating shaft, the lens arm and the motor; the second visual angle adjusting mechanism can flexibly control the movement of the medium microsphere, and the medium microsphere and the second visual angle adjusting mechanism are matched to realize the omnibearing scanning imaging of a sample, and the medium microsphere is recorded by the visual feedback module and fed back to the main control module.
2. Compared with the traditional objective table, the center of the pneumatic objective table is jacked up by the curved surface supporting table, so that the sample is more attached to the medium microsphere. Meanwhile, the medium microsphere film is designed into a bowl shape, so that the sample is covered by the medium microsphere film in all directions, the medium microsphere can be flexibly controlled to move through the second visual angle adjusting mechanism, the sample is subjected to all-direction scanning imaging, and the imaging distortion problem caused by limited angle imaging is avoided.
Drawings
FIG. 1 is a schematic diagram of a super-resolution three-dimensional imaging device based on medium microspheres;
fig. 2 is an enlarged schematic view of a portion of the device within the dashed line box.
The reference numerals shown in fig. 1 to 2 are respectively denoted as: 1-a main control module; 2-a visual feedback module; 3-rotating frame; 4-rotating shaft; 5-ocular; 6-an objective lens; 7-fine Jiao Luoxuan knob; 8-mirror walls; 10-a first viewing angle adjustment mechanism; 11-a second viewing angle adjustment mechanism; 12-medium microspheres; 13-a pneumatic stage; 14-a curved surface support; 15-samples.
Detailed Description
The following description of the embodiments of the present utility model will be made more apparent and fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by a person skilled in the art without any inventive effort, are intended to be within the scope of the present utility model, based on the embodiments of the present utility model.
Referring to fig. 1-2, the utility model provides a super-resolution three-dimensional imaging device based on medium microspheres, which comprises a main control module 1, a visual feedback module 2, a visual angle adjusting mechanism and an image acquisition module, wherein the main control module 1 is respectively in communication connection with the visual feedback module 2 and the visual angle adjusting mechanism, the communication connection mode comprises a WIFI, a bluetooth, 4G, 5G or a wired connection mode, the visual angle adjusting mechanism comprises a first visual angle adjusting mechanism 10 and a second visual angle adjusting mechanism 11, and views acquired by the image acquisition module are transmitted to the main control module 1 through the visual feedback module 2. The main control module 1 is a computer end and is used for receiving the pictures recorded by the visual feedback module 2 and controlling the visual angle adjusting mechanism to adjust the visual angle. The first visual angle adjusting mechanism 10 is used for adjusting the image acquisition module to a proper position, and the second visual angle adjusting mechanism 11 is used for precisely controlling the angles of the medium microsphere 12, the pneumatic object stage 13 and the curved surface supporting table 14.
The image acquisition module comprises a rotating frame 3, a rotating shaft 4 and an acquisition assembly, wherein the rotating frame 3 is rotationally connected with the acquisition assembly through the rotating shaft 4, the first visual angle adjusting mechanism 10 is connected with the rotating frame 3, and the imaging end of the visual feedback module 2 is connected with the acquisition assembly; the second visual angle adjusting mechanism 11 is rotatably connected with a carrying component for placing the sample 15, and the carrying component is positioned below the collecting component and corresponds to the collecting component. The first visual angle adjusting mechanism 10 and the second visual angle adjusting mechanism 11 comprise a motor and a base, the motor of the first visual angle adjusting mechanism 10 is connected with the rotating frame 3 through a shaft sleeve, the motor output end of the second visual angle adjusting mechanism 11 is connected with a driving gear in a matched mode, the end faces of the medium microsphere 12, the pneumatic objective table 13 and the curved surface supporting table 14 are respectively provided with a rotating shaft, and the end portion of the rotating shaft is provided with a driven gear meshed with the driving gear. When the angle of a sample 15 to be observed needs to be adjusted, a driving gear at the output end of a motor of the second visual angle adjusting mechanism 11 drives driven gears on end face rotating shafts of the medium microsphere 12, the pneumatic object stage 13 and the curved surface supporting table 14 to rotate for fine adjustment of the angle, so that the sample 15 reaches a proper observation angle.
The image acquisition module can use a microscope in the prior art, the acquisition component can comprise an ocular 5, an objective lens 6, a fine Jiao Luoxuan knob 7 and an ocular arm 8, focusing is performed by using the fine Jiao Luoxuan knob 7, the rotating frame 3 is connected with the ocular 5 and the objective lens 6 through a rotating shaft 4 to adjust the transverse angle, and the lens of the visual feedback module 2 is connected with the ocular 5.
The carrier component is used for carrying a sample 15, and comprises a medium microsphere 12, a pneumatic object stage 13 and a curved surface supporting table 14, wherein the pneumatic object stage 13 is arranged on the upper surface of the curved surface supporting table 14, the sample 15 is placed on the pneumatic object stage 13, and a medium microsphere film is positioned on the pneumatic object stage 13 and covers the sample 15.
In order to prevent the problem of imaging under multiple visual angles, the device for loading the medium microspheres 12 is subjected to movement control treatment, and the pneumatic object stage 13 and the bowl-shaped medium microsphere film are added, so that the sample 15 can be tightly attached to the medium microspheres and the film, scanning imaging can be realized, and pictures under the two-dimensional imaging effect are obtained. In order to make the sample omnibearing visual display rendering of the 2D picture clearer through technical treatment, and achieve the purpose of generating a new visual angle effect that the sample display has more stereoscopic impression under a visual angle by using a limited shot picture, the picture of two-dimensional observation of an object can be utilized under multiple angles and three-dimensional reconstruction is carried out on the picture, so that the sample imaging picture can have more three-dimensional impression, which is helpful for omnibearing display of the sample 15. Then, the picture is subjected to neural radiation field algorithm (NeRF) three-dimensional reconstruction, which is processed, operated and analyzed by a mathematical model suitable for computer representation and processing in a computer environment, and virtual realization expressing objective world is established in the computer, so that the method is helpful for observation of micro samples. Neural radiation field algorithms (NeRF) are state of the art.
When the medium microsphere 12 is positioned on the surface of the observation sample 15, the information of evanescent waves of an object part is coupled and converted into propagation waves which can be conducted in a far field, so that super-resolution imaging is realized. In order to achieve local information imaging or large area imaging of the sample 15, microsphere movement is required. The medium microsphere 12 is packaged into a bowl shape, so that the sample 15 can be covered by the medium microsphere film in all directions by the design, and the rotation of the sample can be precisely controlled by the operation table.
The pneumatic objective table 13 adopts pneumatic design, and the surface of the objective table is slightly raised by the curved surface supporting table 14 controlled by voltage, so that the sample 15 can be better attached to the medium microsphere 12, scanning imaging is realized on the sample 15, and imaging distortion is prevented. The motor of the second visual angle adjusting mechanism 11 is finely controlled, so that the rotation of the medium microsphere 12, the film, the pneumatic object stage 13 and the curved surface supporting table 14 is controlled to finish the scanning imaging work at different angles, and further, the occurrence of distortion of partial pictures is prevented.
The implementation steps of the utility model are as follows: the sample 15 is placed between the medium microsphere 12 and the pneumatic object stage 13, the positions of the rotating frame 3 and the rotating shaft 4 are adjusted through the motor of the first visual angle adjusting mechanism 10, the acquisition assembly is rotated to different angles, the rotation of the medium microsphere 12, the pneumatic object stage 13 and the curved surface supporting table 14 is controlled through the motor of the second visual angle adjusting mechanism 11, the sample 15 is rotated to a proper angle, the fine adjustment is performed on the lens of the acquisition assembly through the fine adjustment Jiao Luoxuan knob 7, in the process, the vision feedback module 2 is used for photographing every time the position needing photographing is adjusted, the acquired photograph is transmitted to the main control module 1, and the three-dimensional reconstruction is performed through the nerve radiation field algorithm (NeRF).
The foregoing description of the preferred embodiments of the utility model is not intended to limit the utility model to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the utility model are intended to be included within the scope of the utility model.
Claims (5)
1. A super-resolution three-dimensional imaging device based on medium microspheres, comprising: the visual angle adjusting device comprises a main control module (1), a visual feedback module (2), a visual angle adjusting mechanism and an image acquisition module, wherein the main control module (1) is respectively in communication connection with the visual feedback module (2) and the visual angle adjusting mechanism, and the visual angle adjusting mechanism comprises a first visual angle adjusting mechanism (10) and a second visual angle adjusting mechanism (11); transmitting the view acquired by the image acquisition module to a main control module through the visual feedback module (2);
the image acquisition module comprises a rotating frame (3), a rotating shaft (4) and an acquisition assembly, wherein the rotating frame (3) is rotationally connected with the acquisition assembly through the rotating shaft (4), the first visual angle adjusting mechanism (10) is connected with the rotating frame (3), and an imaging end of the visual feedback module (2) is connected with the acquisition assembly;
the second visual angle adjusting mechanism (11) is rotatably connected with a carrying component for placing a sample (15), and the carrying component is positioned below the collecting component and corresponds to the collecting component.
2. The super-resolution three-dimensional imaging device based on medium microspheres according to claim 1, wherein the carrier assembly comprises medium microspheres (12), a pneumatic stage (13) and a curved support table (14), wherein the surface of the medium microspheres (12) is encapsulated with a film, the pneumatic stage (13) is arranged on the upper surface of the curved support table (14), a sample (15) is placed on the pneumatic stage (13), and the medium microsphere film is positioned on the pneumatic stage (13) and covers the sample (15).
3. The super-resolution three-dimensional imaging device based on medium microspheres according to claim 1, wherein the image acquisition module further comprises a mirror arm (8), the mirror arm (8) being connected with the turret (3).
4. The super-resolution three-dimensional imaging device based on medium microspheres according to claim 3, wherein the first visual angle adjusting mechanism (10) and the second visual angle adjusting mechanism (11) comprise motors, the motors of the first visual angle adjusting mechanism (10) are connected with the rotating frame (3) through shaft sleeves, the motor output end of the second visual angle adjusting mechanism (11) is connected with a driving gear in a matched mode, the end faces of the medium microspheres (12), the pneumatic object table (13) and the curved surface supporting table (14) are respectively provided with rotating shafts, and driven gears meshed with the driving gears are arranged at the end parts of the rotating shafts.
5. The super-resolution three-dimensional imaging device based on medium microspheres according to claim 2, wherein the medium microsphere thin film is bowl-shaped in shape.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202322004107.0U CN220340031U (en) | 2023-07-27 | 2023-07-27 | Super-resolution three-dimensional imaging device based on medium microspheres |
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| CN202322004107.0U CN220340031U (en) | 2023-07-27 | 2023-07-27 | Super-resolution three-dimensional imaging device based on medium microspheres |
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| CN220340031U true CN220340031U (en) | 2024-01-12 |
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