CN220317794U - Stem cell autosegregation filter equipment - Google Patents

Abstract

The utility model provides an automatic stem cell separation and filtration device, which relates to the technical field of stem cell separation equipment and comprises a filtration table, an electro-hydraulic push rod and a rotating shaft fixing frame, wherein the filtration table is fixedly connected with supporting legs and is fixedly connected with the electro-hydraulic push rod, the electro-hydraulic push rod is fixedly connected with the rotating shaft fixing frame, the rotating shaft fixing frame is fixedly connected with a motor fixing frame, and the motor fixing frame is fixedly connected with a driving motor. According to the utility model, after the filter box mechanism is installed and fixed, the electro-hydraulic push rod is shortened, the connecting rotating shaft drives the first clamping head to downwards engage with the second clamping head, after engagement is completed, the connecting rotating shaft and the first clamping head are driven by the driving motor to rotate, so that the placement box is driven to rotate rapidly, other substances in stem cells in the filter box mechanism are filtered from the stem cell filtering membrane, and the stem cells can be filtered and separated rapidly, efficiently and sufficiently under the action of centrifugal force, so that the filtering and separating efficiency is improved.

Description

Stem cell autosegregation filter equipment

Technical Field

The utility model relates to the technical field of stem cell separation equipment, in particular to an automatic stem cell separation and filtration device.

Background

At present, stem cells refer to primitive cells with self-renewal and multidirectional differentiation potential, are originating cells of an organism, can be differentiated into one or more cells constituting human tissues or organs under specific conditions, and are required to be filtered and separated during cultivation.

For example, chinese patent number is: the 'SVF cell automatic separation device' of CN206654917U comprises a rack, wherein a liquid adding component, a main body tank, a filtering device and a power device are arranged on the rack, the liquid adding component is connected with a main body tank through a conduit, the main body tank is divided into two paths of conduits which are connected with the power device, one path of conduit is directly connected with a power device and then is connected with a collector, and the other path of conduit is connected with the power device after passing through the filtering device and then is connected with a waste liquid cylinder. The device can ensure that SVF cells are fully automatically collected under the whole-course closed and aseptic conditions. The device has reasonable structural design, convenient and simple use and controllable separation process, greatly saves separation time, improves separation efficiency, ensures activity of the adipose-derived stem cells and has high separation rate. Accordingly, the inventor also establishes a corresponding use method, and the management and industrialization of large-scale separation and extraction of SVF cells can be realized by applying the automatic separation device and the method thereof.

In the prior art, when stem cells are separated and filtered, the stem cells and substances in the stem cells are mostly adopted for gravity flow filtration, so that the filtering efficiency is poor, the stem cells are inconvenient to rapidly and sufficiently filter and separate, and operators need to consume more time to separate and filter the stem cells.

Disclosure of Invention

The utility model aims to solve the problems that in the prior art, when stem cells are separated and filtered, the stem cells and substances in the stem cells are mostly adopted for gravity flow filtration, the filtration efficiency is poor, and the stem cells are inconvenient to rapidly and sufficiently filter and separate, so that operators need to consume more time to separate and filter the stem cells.

In order to achieve the above purpose, the present utility model adopts the following technical scheme: the utility model provides a stem cell autosegregation filter device, includes filter stand, electrohydraulic push rod and pivot mount, filter stand and supporting leg fixed connection, and filter stand and electrohydraulic push rod fixed connection, electrohydraulic push rod and pivot mount fixed connection, pivot mount and motor mount fixed connection, motor mount and driving motor fixed connection, centrifugal separation filter mechanism is including connecting pivot, chuck and fixed slot, connect pivot and driving motor fixed connection, and connect pivot and pivot mount rotation connection, chuck and connection pivot fixed connection, and chuck and No. two chucks intermeshing, fixed slot and filter stand fixed connection, and fixed slot and connecting plate rotation connection, connecting plate and placement box fixed connection, placement box and case lid rotation connection, case lid and No. two chucks fixed connection.

Preferably, the bottom of the filtering table is provided with four supporting legs, and the four supporting legs are distributed on the bottom surface of the filtering table in a rectangular shape.

Preferably, four electro-hydraulic push rods are arranged at the top of the filtering table, and the four electro-hydraulic push rods are distributed on the top surface of the electro-hydraulic push rod in a rectangular shape.

Preferably, the placement box is fixedly connected with a fixing plate, and the fixing plate is fixedly connected with a first rubber pad.

Preferably, the center position of the top of the box cover is fixedly connected with the second clamping head, and the box cover is fixedly connected with the second anti-skid rubber pad.

Preferably, the second anti-slip rubber pad is tightly extruded and fixed with the upper surface of the filter box mechanism, and the bottom surface of the filter box mechanism is tightly extruded and fixed with the top surface of the first rubber pad.

Preferably, a stem cell filtering membrane is arranged inside the filtering box mechanism, and a plurality of through holes are formed in the surface of the filtering box mechanism.

Compared with the prior art, the utility model has the advantages and positive effects that:

1. according to the utility model, stem cells to be filtered and separated are placed in the filter box mechanism, the filter box mechanism is protected on the surface of the fixing plate, the filter box mechanism is in threaded connection with the placement box through the box cover, the second anti-slip rubber pad presses the surface of the filter box mechanism downwards, the friction force on the clamping and fixing position of the filter box mechanism is improved through the first rubber pad and the second anti-slip rubber pad, and the filter box mechanism is convenient to fix stably.

2. According to the utility model, after the filter box mechanism is installed and fixed, the electro-hydraulic push rod is shortened, the connecting rotating shaft drives the first clamping head to downwards engage with the second clamping head, after engagement is completed, the connecting rotating shaft and the first clamping head are driven to rotate by the driving motor, so that the placing box is driven to rotate rapidly, other substances in stem cells in the filter box mechanism are filtered from the stem cell filtering membrane, and the stem cells can be filtered and separated rapidly, efficiently and sufficiently under the action of centrifugal force, so that the filtering and separating efficiency is improved.

Drawings

Fig. 1 is a perspective view showing an automatic stem cell separation and filtration device according to the present utility model;

FIG. 2 is a side perspective view of an automatic stem cell separation filter according to the present utility model;

FIG. 3 is a perspective view showing a placement box of an automatic stem cell separation and filtration device according to the present utility model;

fig. 4 is a schematic cross-sectional view of a placement box of an automatic stem cell separation and filtration device according to the present utility model.

Legend description: 1. a filter stage; 2. support legs; 3. an electro-hydraulic push rod; 4. a rotating shaft fixing frame; 5. a motor fixing frame; 6. a driving motor; 7. the connecting rotating shaft; 8. a first clamping head; 9. a fixing groove; 10. a connecting plate; 11. placing a box; 12. a case cover; 13. a second clamping head; 14. a fixing plate; 15. a first rubber pad; 16. a filter cartridge mechanism; 17. and a second anti-skid rubber pad.

Detailed Description

In order that the above objects, features and advantages of the utility model will be more clearly understood, a further description of the utility model will be rendered by reference to the appended drawings and examples. It should be noted that, in the case of no conflict, the embodiments of the present application and the features in the embodiments may be combined with each other.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present utility model, however, the present utility model may be practiced otherwise than as described herein, and therefore the present utility model is not limited to the specific embodiments of the disclosure that follow.

Referring to fig. 1-4, the present utility model provides a technical solution: an automatic stem cell separation and filtration device comprises a filtration table 1, an electro-hydraulic push rod 3 and a rotating shaft fixing frame 4, wherein the filtration table 1 is fixedly connected with a supporting leg 2, the filtration table 1 is fixedly connected with the electro-hydraulic push rod 3, the electro-hydraulic push rod 3 is fixedly connected with the rotating shaft fixing frame 4, the rotating shaft fixing frame 4 is fixedly connected with a motor fixing frame 5, the motor fixing frame 5 is fixedly connected with a driving motor 6, a centrifugal separation and filtration mechanism comprises a connecting rotating shaft 7, a first clamping head 8 and a fixed groove 9, the connecting rotating shaft 7 is fixedly connected with the driving motor 6, the connecting rotating shaft 7 is rotationally connected with the rotating shaft fixing frame 4, the first clamping head 8 is fixedly connected with the connecting rotating shaft 7, the first clamping head 8 is meshed with a second clamping head 13, the fixed groove 9 is fixedly connected with the filtration table 1, the fixed groove 9 is rotationally connected with a connecting plate 10, the connecting plate 10 is fixedly connected with a placing box 11, the placing box 11 is rotationally connected with the box cover 12, the box cover 12 is fixedly connected with the second clamping head 13, stem cells which need to be filtered and separated are placed in the filtering box mechanism 16, the filtering box mechanism 16 is protected on the surface of the fixed plate 14, the box cover 12 is in threaded connection with the placing box 11, the second anti-slip rubber pad 17 presses the surface of the filtering box mechanism 16 downwards, the friction force of the clamping and fixing position of the filtering box mechanism 16 is improved through the first rubber pad 15 and the second anti-slip rubber pad 17, the filtering box mechanism 16 is stably fixed, after the filtering box mechanism 16 is installed and fixed, the electro-hydraulic push rod 3 is shortened, the connecting rotating shaft 7 drives the first clamping head 8 to downwards engage and plug-in with the second clamping head 13, after engagement is completed, the connecting rotating shaft 7 and the first clamping head 8 are driven to rotate through the driving motor 6, so that the placing box 11 is driven to rotate rapidly, at this time, other substances in the stem cells inside the filter box mechanism 16 are filtered from the stem cell filtering membrane, and under the action of centrifugal force, the stem cells can be filtered and separated quickly, efficiently and sufficiently, so that the filtering and separating efficiency is improved.

The bottom of the filtering table 1 is provided with four supporting legs 2, the four supporting legs 2 are distributed on the bottom surface of the filtering table 1 in a rectangular shape, and the four supporting legs 2 are convenient for stably supporting the surface of the filtering table 1.

Four electro-hydraulic push rods 3 are arranged at the top of the filtering table 1, the four electro-hydraulic push rods 3 are distributed on the top surface of the electro-hydraulic push rods 3 in a rectangular shape, and the upper structure of the filtering table is convenient to stably push through the four electro-hydraulic push rods 3.

Place case 11 and fixed plate 14 fixed connection, fixed plate 14 and rubber pad 15 fixed connection, case lid 12 top central point puts and No. two dop 13 fixed connection, and case lid 12 and No. two anti-skidding rubber pad 17 fixed connection, no. two anti-skidding rubber pad 17 and the inseparable extrusion of filtration box mechanism 16 upper portion surface are fixed, filtration box mechanism 16 bottom surface and No. one rubber pad 15 top surface are closely extruded fixedly, filtration box mechanism 16 inside is provided with stem cell filtration membrane, and filtration box mechanism 16 surface is provided with a plurality of through-hole, improve the frictional force to the tight fixed part of filtration box mechanism 16 clamp through No. one rubber pad 15 and No. two anti-skidding rubber pads 17, be convenient for carry out stable fixed to filtration box mechanism 16, other substances in the stem cell of filtration box mechanism 16 inside are from stem cell filtration membrane filtered the department, through centrifugal force effect, be convenient for carry out quick, high efficiency, abundant filtration separation to the stem cell, and improvement filtration separation efficiency.

The application method and the working principle of the device are as follows: the stem cells needing to be filtered and separated are placed inside the filter box mechanism 16, the filter box mechanism 16 is protected on the surface of the fixed plate 14, the filter box mechanism 16 is connected with the placement box 11 through the box cover 12 in a threaded mode, the second anti-slip rubber pad 17 presses the surface of the filter box mechanism 16 downwards, the friction force of the clamping and fixing position of the filter box mechanism 16 is improved through the first anti-slip rubber pad 15 and the second anti-slip rubber pad 17, the filter box mechanism 16 is stabilized and fixed, after the filter box mechanism 16 is installed and fixed, the electro-hydraulic push rod 3 is shortened, the connecting rotating shaft 7 drives the first clamping head 8 to be meshed and inserted downwards with the second clamping head 13, after meshing is completed, the connecting rotating shaft 7 and the first clamping head 8 are driven to rotate through the driving motor 6, so that the placement box 11 is driven to rotate rapidly, and other substances in the stem cells inside the filter box mechanism 16 are filtered from the stem cell filtering membrane, rapid, efficient and sufficient filtering separation is facilitated through centrifugal force, and filtering separation efficiency is improved.

The present utility model is not limited to the above-mentioned embodiments, and any equivalent embodiments which can be changed or modified by the technical content disclosed above can be applied to other fields, but any simple modification, equivalent changes and modification made to the above-mentioned embodiments according to the technical substance of the present utility model without departing from the technical content of the present utility model still belong to the protection scope of the technical solution of the present utility model.

Claims (7)

1. The utility model provides a stem cell autosegregation filter device, includes filter stand (1), electrohydraulic push rod (3) and pivot mount (4), filter stand (1) and supporting leg (2) fixed connection, and filter stand (1) and electrohydraulic push rod (3) fixed connection, electrohydraulic push rod (3) and pivot mount (4) fixed connection, pivot mount (4) and motor mount (5) fixed connection, motor mount (5) and driving motor (6) fixed connection, its characterized in that: centrifugal separation filter mechanism, centrifugal separation filter mechanism is including connecting pivot (7), chuck (8) and fixed slot (9), connect pivot (7) and driving motor (6) fixed connection, and connect pivot (7) and pivot mount (4) rotation connection, chuck (8) and connect pivot (7) fixed connection, and chuck (8) and No. two chucks (13) intermeshing, fixed slot (9) and filter table (1) fixed connection, and fixed slot (9) and connecting plate (10) rotation connection, connecting plate (10) and placement box (11) fixed connection, placement box (11) and case lid (12) rotation connection, case lid (12) and No. two chucks (13) fixed connection.

2. The stem cell automatic separation filter device according to claim 1, wherein: the bottom of the filtering table (1) is provided with four supporting legs (2), and the four supporting legs (2) are distributed on the bottom surface of the filtering table (1) in a rectangular shape.

3. The stem cell automatic separation filter device according to claim 1, wherein: four electro-hydraulic push rods (3) are arranged at the top of the filtering table (1), and the four electro-hydraulic push rods (3) are distributed on the top surface of the electro-hydraulic push rods (3) in a rectangular shape.

4. The stem cell automatic separation filter device according to claim 1, wherein: the placing box (11) is fixedly connected with the fixing plate (14), and the fixing plate (14) is fixedly connected with the first rubber pad (15).

5. The stem cell automatic separation filter device according to claim 1, wherein: the center of the top of the box cover (12) is fixedly connected with a second clamping head (13), and the box cover (12) is fixedly connected with a second anti-slip rubber pad (17).

6. The stem cell autosegregation filter device of claim 5, wherein: the second anti-slip rubber pad (17) is tightly extruded and fixed with the upper surface of the filter box mechanism (16), and the bottom surface of the filter box mechanism (16) is tightly extruded and fixed with the top surface of the first rubber pad (15).

7. The stem cell automatic separation filter device according to claim 6, wherein: the stem cell filtering membrane is arranged inside the filtering box mechanism (16), and a plurality of through holes are formed in the surface of the filtering box mechanism (16).