CN220304908U - Device for collecting environmental DNA in turbid water - Google Patents

Abstract

The utility model discloses an environment DNA acquisition device in turbid water, and relates to the technical field of environment monitoring. The utility model comprises a primary filter cabin, an equipment cabin and a secondary filter cabin, wherein a primary filter assembly is clamped in the primary filter cabin, a water pump and a vacuum pump positioned above the water pump are fixed in the equipment cabin, an interface is fixedly penetrated through the center of a partition board, and a glass fiber filter membrane is covered at the upper end of the interface; the primary filter component comprises two vertical rods which are vertically arranged, the peripheries of the two vertical rods are fixedly provided with a lower annular plate and an upper annular plate which is positioned above the lower annular plate, and the upper ends of the upper annular plate and the lower annular plate are fixedly provided with filter screens. According to the utility model, by arranging the primary filter cabin, the equipment cabin, the secondary filter cabin, the primary filter component, the glass fiber filter membrane, the water pump and the vacuum pump, the problem that the microporous filter membrane is easy to be blocked, frequent in replacement and long in suction filtration time when taking the DNA in the water sample environment is solved, and the DNA in the water sample is degraded indirectly; and the problem that the filtering assembly is inconvenient to clean and replace after a large amount of water is filtered.

Description

Device for collecting environmental DNA in turbid water

Technical Field

The utility model belongs to the technical field of environmental monitoring, and particularly relates to an environmental DNA acquisition device in turbid water.

Background

The term "environmental DNA" refers to DNA fragments that can be directly extracted from environmental samples (e.g., water, soil, air, ice core, etc.), and is a mixture of DNA from different species such as microorganisms, animals, plants, etc., and includes both intracellular DNA released from organisms into epidermal cells in the environment via skin, urine, feces, mucus, etc., and extracellular DNA released into the environment by lysis after cell death. DNA is captured from an environmental sample and stored, extracted, amplified, sequenced and classified to determine the distribution of organisms in the sampling environment. In popular terms, DNA is extracted from the water sample after suction filtration, sequencing and identification are carried out, and the data in the library are compared to obtain the distribution and abundance of organisms. However, the following disadvantages still exist during the collection of the environmental DNA in the turbid water body:

the key point of the prior art is how to replace the filter membrane rapidly and efficiently, such as a magazine type filter membrane, a wheel type filter membrane and the like. The method is effective when the suspended particles in the water body are very few, but when the suspended particles are dense when facing a river with high sediment content such as yellow river, a circular microporous filter membrane with the diameter of 5cm needs to be replaced every 30ml to 50ml on average. And when the DNA content of the monitored species is low, the sampling water body is required to be enlarged and the sampling point positions are required to be increased. When these several conditions are overlapped, it is not helpful to replace more filters. Therefore, the problems that microporous filter membranes are easy to block, frequent in replacement and long in suction filtration time when DNA in a water sample environment is taken in a river with high sand or turbidity are solved, and DNA in the water sample is degraded are solved indirectly.

Disclosure of Invention

The utility model aims to provide an environment DNA collecting device in turbid water, which solves the problems that when water sample environment DNA is adopted by conventional means, a microporous filter membrane is easy to block, frequent to replace and overlong in suction filtration time, and DNA in the water sample is degraded indirectly by arranging a primary filter chamber, an equipment chamber, a secondary filter chamber, a primary filter assembly, a glass fiber filter membrane, a water pump and a vacuum pump; and the problem that the filtering assembly is inconvenient to clean and replace after a large amount of water is filtered.

In order to solve the technical problems, the utility model is realized by the following technical scheme:

the utility model relates to an environment DNA acquisition device in turbid water, which comprises a first-stage filter tank, an equipment tank and a second-stage filter tank, wherein the first-stage filter tank, the equipment tank and the second-stage filter tank are arranged side by side, and the equipment tank is fixed between the first-stage filter tank and the second-stage filter tank; the primary filter cabin is internally provided with a primary filter assembly in a clamping manner, a water pump and a vacuum pump positioned above the water pump are fixed in the equipment cabin, a baffle plate is fixed in the middle of an inner cavity of the secondary filter cabin, an interface is fixedly penetrated through the center of the baffle plate, the upper end of the interface is covered with a glass fiber filter membrane, a connecting pipe is screwed outside the interface, and a funnel is fixed at the upper end of the connecting pipe;

the primary filter cabin is used for carrying out preliminary filtration on turbid water, a preliminary filter assembly is arranged in the primary filter cabin to carry out preliminary filtration to obtain a large number of impurity samples for subsequent eDNA detection, various mechanical driving devices such as a water pump are arranged in the equipment cabin to pump water, a vacuum pump is used for carrying out vacuum pumping and filtering, the suction filtration process is accelerated, a glass fiber filter membrane is arranged in the secondary filter cabin and is used for carrying out secondary filtration on the water after water filtration, the water samples after filtration and the filtered impurity samples are quickly obtained after the filtration are filtered again through the glass fiber filter membrane after the filtration of the preliminary filter assembly, and the problem of DNA degradation in a water sample is reduced;

the primary filter assembly comprises two vertical rods which are vertically arranged, wherein the peripheries of the two vertical rods are fixedly provided with a lower annular plate and an upper annular plate positioned above the lower annular plate, and the upper ends of the upper annular plate and the lower annular plate are fixedly provided with filter screens;

the primary filter component adopts conventional filter screen type filtration, and is matched with a subsequent glass fiber filter membrane to carry out secondary filtration, so that the traditional microporous filter membrane filtration treatment is changed, the upper annular plate and the lower annular plate are both provided with filter screens, and the filter screens with different meshes are matched to carry out double filtration treatment to filter a large amount of suspended particles in the water body;

the peripheries of the upper ring plate and the lower ring plate are fixed with symmetrical clamping blocks, and the clamping blocks are slidably clamped in clamping grooves vertically formed in the inner wall of the primary filter cabin; go up the crown block and lower crown block all through the fixture block joint in the draw-in groove, make it firmly restrict in the one-level filter cabin when filtering, secondly go up crown block and lower crown block and two montants are fixed, guarantee overall structure stability, it is simpler when taking out, mention two montants, can be with last crown block, lower crown block and the filter screen of its top and the impurity that is filtered out by the filter screen are synchronous once only taken out, obtain a large amount of environment samples fast, carry out eDNA to this impurity of filtering out and detect, and change the clearance and be simpler and more convenient.

Further, the upper end of the primary filter cabin is screwed with a cabin cover I, the top end of the cabin cover I is fixedly penetrated by an air valve, and the top end of the cabin cover I is also fixedly provided with a water inlet pipe with a valve;

the first-stage filter cabin is closed by the cabin cover, the air pressure in the first-stage filter cabin can be balanced with the external environment by opening the air valve, and the air pressure in the first-stage filter cabin is in a negative pressure suction filtration state in a normal state and is input into a turbid water body to be collected through the water inlet pipe.

Further, two exhaust pipes are fixed at the exhaust end of the vacuum pump, one end of the exhaust pipe, which is far away from the vacuum pump, is communicated with the inner cavity of the primary filter cabin below the lower annular plate, the other end of the exhaust pipe, which is far away from the vacuum pump, is communicated with the inner cavity of the secondary filter cabin below the partition plate, and an air outlet pipe is also fixed at the air outlet end of the vacuum pump;

through the work of the vacuum pump, the air in the first-stage filter chamber and the second-stage filter chamber is respectively pumped out by utilizing the two exhaust pipes, and is discharged through the air outlet pipe, so that negative pressure is formed in the inner chambers of the first-stage filter chamber and the second-stage filter chamber, and the water bodies on the filter screen and the glass fiber filter membrane are forced to be filtered quickly, so that a quick suction filtration treatment effect is formed.

Further, a water pumping pipe is fixed at the water pumping end of the water pump, one end of the water pumping pipe, which is far away from the water pump, is communicated with the inner bottom of the inner cavity of the primary filter cabin, a water outlet pipe is fixed at the water outlet end of the water pump, and one end of the water outlet pipe, which is far away from the water pump, is communicated and fixed with a second cabin cover screwed on the top of the secondary filter cabin;

the water pump works, the water body filtered by the primary filtering component is pumped by the water pumping pipe, the water body is guided into the inner cavity of the secondary filtering cabin through the water outlet pipe and is collected by the funnel, the upper end of the secondary filtering cabin is also sealed through the cabin cover II, and the water pumping pipe is connected with the inner cavity of the secondary filtering cabin below the partition plate through the air pumping pipe on the vacuum pump, so that the secondary filtering cabin also has a suction filtering function.

Further, a limiting ring is fixed in the connecting pipe, a sealing gasket is arranged at the bottom of the limiting ring and is abutted against the upper part of the glass fiber filter membrane, the interfaces protrude out of the upper end of the partition plate, and the part of each interface protruding upwards out of the partition plate is screwed in the connecting pipe; the glass fiber filter membrane shell is used for further filtering relevant particle suspended matters in the water body, the impurity sample in the water body sample can be separated again through rapid filtration again, the required impurity sample and the filtered water body sample are rapidly obtained, the water body in the funnel is filtered by the glass fiber filter membrane, the water body enters the inner cavity of the secondary filter chamber below the partition plate through the interface, the water body sample is finally discharged through the drain pipe, and the filtered impurity sample is reserved above the glass fiber filter membrane.

Further, the filter screen on the upper annular plate is 300 meshes, the filter screen on the lower annular plate is 400 meshes, and the filter screen with gradually increased mesh number ensures efficient filtering treatment.

Further, a drain pipe is fixed on one side of the secondary filter cabin far away from the primary filter cabin, and the drain pipe is communicated with the inner cavity of the secondary filter cabin below the partition plate; the drain pipe is used for discharging the water body sample.

The utility model has the following beneficial effects:

according to the utility model, the primary filter chamber, the equipment chamber, the secondary filter chamber, the primary filter assembly, the glass fiber filter membrane, the water pump and the vacuum pump are arranged, so that the problems that when the water sample environment DNA is adopted by the conventional means, the microporous filter membrane is easy to be blocked, the replacement is frequent, the suction filtration time is too long, and the degradation of the DNA in the water sample is indirectly caused are solved; the primary filter assembly adopts conventional filter screen type filtration, filter screens are arranged on an upper annular plate and a lower annular plate, double filtration treatment is carried out by matching with the filter screens with different mesh numbers, a large amount of suspended particles in a water body are filtered, and then the water body is matched with a subsequent glass fiber filter membrane for secondary filtration, and negative pressure suction filtration is carried out by pumping through a vacuum pump, so that the filtration process is accelerated.

The utility model solves the problem that the filter assembly is inconvenient to clean and replace after a large amount of water is filtered by arranging the primary filter assembly; go up the crown block and lower crown block all through the fixture block joint in the draw-in groove, make it firmly restrict in the one-level filter chamber when filtering, secondly go up crown block and lower crown block and two montants are fixed, guarantee overall structure stability, it is simpler when taking out, mention two montants, can be with last crown block, lower crown block and the filter screen of its top and the impurity synchronous disposable that is filtered out by the filter screen take out, change clearance is simpler convenient more, simultaneously can obtain a large amount of environmental samples that are filtered out again fast, the convenience carries out eDNA detection to the impurity that should filter out.

Drawings

FIG. 1 is a perspective view of an environmental DNA collection device in a turbid water body;

FIG. 2 is a block diagram of the hatch after opening;

FIG. 3 is a structural connection diagram of the primary filter pod and the equipment pod in cross section;

FIG. 4 is a cross-sectional view of a primary filter cartridge;

FIG. 5 is a cross-sectional view of the equipment bay;

FIG. 6 is a disassembled view of the primary filter assembly;

FIG. 7 is a cross-sectional view of a secondary filter pod;

FIG. 8 is a cross-sectional connection view of the funnel and connecting tube;

fig. 9 is a connection diagram of the connection pipe and the connector after being cut.

Reference numerals:

1. a first-stage filter chamber; 101. a first hatch cover; 1011. an air valve; 1012. a water inlet pipe; 102. a clamping groove; 2. an equipment compartment; 201. a water pump; 2011. a water pumping pipe; 2012. a water outlet pipe; 202. a vacuum pump; 2021. an exhaust pipe; 2022. an air outlet pipe; 3. a second-stage filter chamber; 301. a second hatch cover; 302. a drain pipe; 303. a partition plate; 3031. an interface; 304. a funnel; 305. a connecting pipe; 3051. a limiting ring; 3052. a sealing gasket; 306. a glass fiber filter membrane; 4. a primary filtration assembly; 401. a vertical rod; 402. a lower ring plate; 403. an upper ring plate; 404. a filter screen; 405. and (5) clamping blocks.

Detailed Description

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model.

Referring to fig. 1-9, the utility model discloses an environmental DNA collection device in turbid water, which comprises a first filter chamber 1, an equipment chamber 2 and a second filter chamber 3, wherein the first filter chamber 1, the equipment chamber 2 and the second filter chamber 3 are arranged side by side, and the equipment chamber 2 is fixed between the first filter chamber 1 and the second filter chamber 3; the primary filter chamber 1 is internally and fixedly connected with a primary filter assembly 4, a water pump 201 and a vacuum pump 202 positioned above the water pump 201 are fixed in the equipment chamber 2, a partition plate 303 is fixed in the middle of an inner cavity of the secondary filter chamber 3, an interface 3031 is fixedly penetrated through the center of the partition plate 303, the upper end of the interface 3031 is covered with a glass fiber filter membrane 306, a connecting pipe 305 is connected with the outside of the interface 3031 in a screwed manner, and a funnel 304 is fixed at the upper end of the connecting pipe 305;

the primary filter cabin 1 is used for preliminarily filtering turbid water, a primary filter assembly 4 is arranged in the primary filter cabin to carry out preliminary filtration to obtain a large number of impurity samples for subsequent eDNA detection, various mechanical driving devices such as a water pump 201 are arranged in the equipment cabin 2 to pump water, a vacuum pump 202 is used for vacuumizing and filtering, the vacuumizing and filtering process is accelerated, a glass fiber filter membrane 306 is arranged in the secondary filter cabin 3 and is used for carrying out secondary filtration on the water after water filtration, the water samples after filtration and the impurity samples after filtration are rapidly obtained after filtration are filtered again through the glass fiber filter membrane 306 after the primary filter assembly 4, and the problem of DNA degradation in the water sample is reduced;

the primary filter assembly 4 comprises two vertical rods 401 which are vertically arranged, wherein the peripheries of the two vertical rods 401 are fixedly provided with a lower annular plate 402 and an upper annular plate 403 positioned above the lower annular plate 402, and the upper ends of the upper annular plate 403 and the lower annular plate 402 are fixedly provided with a filter screen 404;

the primary filter assembly 4 adopts a conventional filter screen 404 for filtering, and is matched with a subsequent glass fiber filter membrane 306 for filtering again, so that the conventional microporous filter membrane filtering treatment is changed, the upper annular plate 403 and the lower annular plate 402 are both provided with the filter screens 404, and the filter screens 404 with different meshes are matched for double filtering treatment to filter a large amount of particle suspended matters in the water body;

symmetrical clamping blocks 405 are fixed on the peripheries of the upper ring plate 403 and the lower ring plate 402, and the clamping blocks 405 are slidably clamped in clamping grooves 102 vertically formed in the inner wall of the primary filter tank 1; go up the crown plate 403 and go down crown plate 402 and all pass through fixture block 405 joint in draw-in groove 102, make it firmly restrict in one-level filter capsule 1 when filtering, secondly go up crown plate 403 and lower crown plate 402 and two montants 401 are fixed, guarantee that overall structure is stable, it is simpler when taking out, mention two montants 401, can be with last crown plate 403, lower crown plate 402 and the filter screen 404 of its top and the impurity that is filtered out by filter screen 404 are synchronous disposable take out, obtain a large amount of environmental samples fast, carry out eDNA detection to this impurity of filtering out, and change the clearance and be simpler and more convenient.

The upper end of the primary filter cabin 1 is screwed with a cabin cover I101, the top end of the cabin cover I101 is fixedly penetrated by an air valve 1011, and the top end of the cabin cover I101 is also fixedly provided with a water inlet pipe 1012 with a valve;

the first cabin cover 101 seals the upper end of the first-stage filter cabin 1, the air pressure in the first-stage filter cabin 1 can be balanced with the external environment by opening the air valve 1011, and the air pressure is in a negative pressure suction filtration state in a normal state, and turbid water needing to be collected is input through the water inlet pipe 1012.

Two air extraction pipes 2021 are fixed at the air extraction end of the vacuum pump 202, one end of one air extraction pipe 2021 far away from the vacuum pump 202 is communicated with the inner cavity of the primary filter cabin 1 below the lower annular plate 402, the other end of the other air extraction pipe 2021 far away from the vacuum pump 202 is communicated with the inner cavity of the secondary filter cabin 3 below the partition plate 303, and an air outlet pipe 2022 is also fixed at the air outlet end of the vacuum pump 202;

through the work of the vacuum pump 202, the air in the inner cavities of the primary filter chamber 1 and the secondary filter chamber 3 is respectively pumped out by utilizing the two exhaust pipes 2021 and is exhausted through the air outlet pipe 2022, so that negative pressure is formed in the inner cavities of the primary filter chamber 1 and the secondary filter chamber 3, and the water bodies on the filter screen 404 and the glass fiber filter membrane 306 are forced to be filtered quickly, so that a quick suction filtration treatment effect is formed.

A water pumping pipe 2011 is fixed at the water pumping end of the water pump 201, one end of the water pumping pipe 2011, which is far away from the water pump 201, is communicated with the inner bottom of the inner cavity of the primary filter cabin 1, a water outlet pipe 2012 is fixed at the water outlet end of the water pump 201, and one end of the water outlet pipe 2012, which is far away from the water pump 201, is communicated and fixed with a second cabin cover 301 screwed on the top of the secondary filter cabin 3;

the water pump 201 works, the water body filtered by the primary filter assembly 4 is pumped by the water pumping pipe 2011, is guided into the inner cavity of the secondary filter cabin 3 through the water outlet pipe 2012, is taken in by the funnel 304, the upper end of the secondary filter cabin 3 is also sealed through the cabin cover II 301, and the secondary filter cabin 3 also has a suction filtration function by connecting the air pumping pipe 2021 on the vacuum pump 202 and penetrating through the inner cavity of the secondary filter cabin 3 below the partition plate 303.

A limiting ring 3051 is fixed in the connecting pipe 305, a sealing gasket 3052 is arranged at the bottom of the limiting ring 3051, the sealing gasket 3052 is abutted against the upper part of the glass fiber filter membrane 306, the interfaces 3031 protrude out of the upper end of the partition plate 303, and the part of each interface 3031 protruding upwards out of the partition plate 303 is screwed in the connecting pipe 305; the glass fiber filter membrane 306 shell further filters relevant particle suspended matters in the water body, the impurity sample in the water body sample can be separated again through rapid filtration again, the required impurity sample and the filtered water body sample can be obtained rapidly, the water body entering the funnel 304 is filtered by the glass fiber filter membrane 306, enters the inner cavity of the secondary filter chamber 3 below the partition plate 303 through the interface 3031, the water body sample is finally discharged through the drain pipe 302, and the filtered impurity sample is reserved above the glass fiber filter membrane 306.

The filter screen 404 on the upper ring plate 403 is 300 meshes, the filter screen 404 on the lower ring plate 402 is 400 meshes, and the filter screen 404 with gradually increased mesh number ensures efficient filtering treatment.

A drain pipe 302 is also fixed on one side of the secondary filter chamber 3 far away from the primary filter chamber 1, and the drain pipe 302 is communicated with the inner cavity of the secondary filter chamber 3 below the partition plate 303; drain 302 is used to drain the water sample.

The specific working principle of the utility model is as follows: the turbid water to be collected is input through a water inlet pipe 1012, enters the first-stage filter capsule 1, is filtered by a filter screen 404 arranged on an upper annular plate 403 and a lower annular plate 402, is subjected to double filtration by matching with the filter screens 404 with different meshes, filters a large amount of suspended particles in the water, simultaneously, the air in the inner cavities of the first-stage filter capsule 1 and the second-stage filter capsule 3 is pumped out through a suction pipe 2021 by the work of a vacuum pump 202, the air in the inner cavities of the first-stage filter capsule 1 and the second-stage filter capsule 3 is discharged through an air outlet pipe 2022, so that negative pressure is formed in the inner cavities of the first-stage filter capsule 1 and the second-stage filter capsule 3, the water on the filter screen 404 is forced to be rapidly filtered, a rapid suction filtration effect is formed, the water sample filtered by the primary filter assembly 4 is pumped by a water pump 201, the water filtered by the primary filter assembly 4 is pumped by a water pump 2011, is guided into the inner cavity of the second-stage filter capsule 3 by a water outlet pipe 2012, the upper end of the second-stage filter capsule 3 is also sealed by a capsule cover 301, then is received by a funnel 304, filtered by a glass fiber filter membrane 306, and finally enters the inner cavity of the second-stage drain pipe 302 below a partition board 303, and finally discharged by the water body 302; the whole process is carried out in a closed space for filtration treatment, so that exogenous pollution to a sample is avoided;

the final samples required for detection are:

1. the impurity samples remained on the two filter screens 404 are used for extracting DNA by using a kit, and then sequencing is used;

2. the impurity sample which is filtered by the glass fiber filter membrane 306 and remains on the glass fiber filter membrane 306 is also used for eDNA detection;

3. the water sample discharged through the water discharge pipe 302 can be used for measuring physical and chemical parameters of water.

The foregoing is only a preferred embodiment of the present utility model, and the present utility model is not limited thereto, and any modification, equivalent replacement, and improvement of some of the technical features described in the foregoing embodiments are all within the scope of the present utility model.

Claims (7)

1. The utility model provides an environment DNA collection system in muddy water body, includes one-level filter capsule (1), equipment compartment (2) and second grade filter capsule (3), its characterized in that: the first-stage filter chamber (1), the equipment chamber (2) and the second-stage filter chamber (3) are arranged side by side, and the equipment chamber (2) is fixed between the first-stage filter chamber (1) and the second-stage filter chamber (3); the primary filter cabin (1) is internally clamped with a primary filter assembly (4), a water pump (201) and a vacuum pump (202) positioned above the water pump (201) are fixedly arranged in the equipment cabin (2), a partition plate (303) is fixedly arranged in the middle of an inner cavity of the secondary filter cabin (3), an interface (3031) is fixedly penetrated through the center of the partition plate (303), a glass fiber filter membrane (306) is covered at the upper end of the interface (3031), a connecting pipe (305) is externally connected with the interface (3031), and a funnel (304) is fixedly arranged at the upper end of the connecting pipe (305);

the primary filter assembly (4) comprises two vertical rods (401) which are vertically arranged, a lower annular plate (402) and an upper annular plate (403) which is positioned above the lower annular plate (402) are jointly fixed on the periphery of the two vertical rods (401), and filter screens (404) are fixed at the upper ends of the upper annular plate (403) and the lower annular plate (402);

the peripheries of the upper annular plate (403) and the lower annular plate (402) are both fixed with symmetrical clamping blocks (405), and the clamping blocks (405) are slidably clamped in clamping grooves (102) vertically formed in the inner wall of the primary filter chamber (1).

2. The device for collecting environmental DNA in turbid water according to claim 1, wherein: the upper end of the primary filter cabin (1) is screwed with a first cabin cover (101), an air valve (1011) is fixedly penetrated at the top end of the first cabin cover (101), and a water inlet pipe (1012) with a valve is also fixedly arranged at the top end of the first cabin cover (101).

3. The device for collecting environmental DNA in turbid water according to claim 1, wherein: two exhaust pipes (2021) are fixed at the exhaust end of the vacuum pump (202), one end of one exhaust pipe (2021) far away from the vacuum pump (202) is communicated with the inner cavity of the primary filter cabin (1) below the lower annular plate (402), one end of the other exhaust pipe (2021) far away from the vacuum pump (202) is communicated with the inner cavity of the secondary filter cabin (3) below the partition plate (303), and the air outlet end of the vacuum pump (202) is also fixed with an air outlet pipe (2022).

4. The device for collecting environmental DNA in turbid water according to claim 1, wherein: the water pumping end of the water pump (201) is fixedly provided with a water pumping pipe (2011), one end, far away from the water pump (201), of the water pumping pipe (2011) is communicated with the inner bottom of the inner cavity of the primary filter cabin (1), the water outlet end of the water pump (201) is fixedly provided with a water outlet pipe (2012), and one end, far away from the water pump (201), of the water outlet pipe (2012) is communicated with a second cabin cover (301) which is screwed with the top of the secondary filter cabin (3).

5. The device for collecting environmental DNA in turbid water according to claim 1, wherein: the connecting pipe (305) is internally fixed with a limiting ring (3051), the bottom of the limiting ring (3051) is provided with a sealing gasket (3052), the sealing gasket (3052) is abutted above the glass fiber filter membrane (306), the interfaces (3031) protrude out of the upper end of the partition plate (303), and the part of each interface (3031) protruding upwards out of the partition plate (303) is screwed in the connecting pipe (305).

6. The device for collecting environmental DNA in turbid water according to claim 1, wherein: the filter screen (404) on the upper annular plate (403) is 300 meshes, and the filter screen (404) on the lower annular plate (402) is 400 meshes.

7. The device for collecting environmental DNA in turbid water according to claim 1, wherein: a drain pipe (302) is further fixed on one side, far away from the primary filter cabin (1), of the secondary filter cabin (3), and the drain pipe (302) is communicated with the inner cavity of the secondary filter cabin (3) below the partition plate (303).