CN220300689U

CN220300689U - Converter and separation device with same

Info

Publication number: CN220300689U
Application number: CN202321119028.8U
Authority: CN
Inventors: 张统; 赵军; 崔鑫; 段红平; 张国强; 董骧
Original assignee: Beijing Naton Medical Technology Holdings Co Ltd
Current assignee: Beijing Naton Medical Technology Holdings Co Ltd
Priority date: 2023-05-10
Filing date: 2023-05-10
Publication date: 2024-01-05
Anticipated expiration: 2033-05-10

Abstract

The present utility model relates to a converter and a separating device having the same, the converter comprising: the device comprises a shell, a conversion tube, an adjusting assembly and a positioning assembly. The switching tube is worn to locate the casing, the switching tube has an adjustment section, the adjustment section is located in the casing, the tip of switching tube is used for linking to each other with the syringe, adjusting part with the adjustment section links to each other, adjusting part is relative the casing is movable, in order to change the circulation clearance of adjusting the section, locating component locates the casing with on one of adjusting part, locating component can with the casing with another one of adjusting part offsets, in order to with adjusting part location to locking gear. The converter provided by the utility model can improve the yield and activity of SVF cells, has excellent separation effect and can be accurately regulated.

Description

Converter and separation device with same

Technical Field

The utility model relates to the technical field of medical equipment, in particular to a converter and a separation device with the same.

Background

Adipose tissue is used as a source of adult stem cells and can be used in plastic surgery and regenerative medicine. The vascular matrix component (SVF) isolated from adipose tissue is a heterogeneous cell population, and the important components contained in the matrix vascular component (SVF) are mainly adipose mesenchymal stem cells, endothelial progenitor cells, endothelial cells, regulatory T cells, macrophages, smooth muscle cells, pericytes, preadipocytes and the like. Among the main components of SVF described above, adipose mesenchymal stem cells (ADSCs) have self-replicative capacity and multipotency, and have chemotaxis, homing, migration and low antigenicity, and can differentiate into bone cells, cartilage cells, muscle cells, hematopoietic stromal cells, nerve cells, and the like.

In the related art, the preparation process of SVF cells mainly includes an enzymatic digestion method and a mechanical emulsification method. Enzymatic digestion separates the contents into two distinct phases by digesting the fat portion of the fat aspirate with collagenase: mature adipocyte fraction and target cell fraction, and then obtaining SVF cells by centrifugation. However, the enzyme digestion method has problems of excessive treatment of tissue cells and enzyme residue clinically, and the enzyme digestion time is too long, which results in complicated operation steps and high cost and has a safety hazard.

In the mechanical emulsification method, the SVF cell yield prepared by using the Closed tube equipment of Tiryaki company is 1.34×10 ⁶ cell/mL, SVF cell viability was 85.82%.

As an example, the Fatstem kit apparatus from CORIOS Soc.Coop, which produces SVF cells with a yield of 3X 10 ⁴ cell/mL, SVF cell viability was 52.00%.

Taking myStem equipment from myStem LLC as an example, SVF cells were prepared with a yield of 8X 10 ³ cell/mL, SVF cell viability was 43.00%.

Among them, most of the fat converters in the mechanical emulsification method are designed with hollow blades or fine wire mesh structures, and the extracted fat extract and fat cells are usually pushed back and forth by means of a syringe and broken, thinned and emulsified by passing through the hollow blades or fine wire mesh holes of the fat converters. However, while adipose tissue and fat cells are disrupted, refined and emulsified, SVF cells entrapped within the fat particles and dispersed among the fat cells are chopped or crushed together. On the other hand, the gear adjustment of the fat transformer in the related art is poor in accuracy, thus resulting in low SVF cell yield and poor cell viability.

Disclosure of Invention

The present utility model aims to solve at least one of the technical problems in the related art to some extent.

Therefore, the embodiment of the utility model provides a converter which can improve the yield and the activity of SVF cells, has excellent separation effect and can be accurately regulated.

The embodiment of the utility model provides a separation device.

The converter of the embodiment of the utility model comprises: a housing; the conversion tube penetrates through the shell and is provided with an adjusting section, the adjusting section is positioned in the shell, and the end part of the conversion tube is connected with the injector; the adjusting assembly is connected with the adjusting section and can move relative to the shell so as to change the circulation gap of the adjusting section; the positioning component is arranged on one of the shell and the adjusting component, and can be propped against the other of the shell and the adjusting component to position the adjusting component to a locking gear.

According to the converter provided by the embodiment of the utility model, the fat extract to be separated can be pushed through the conversion tube repeatedly through the injector, and the circulation gap of the adjusting section can be adjusted to the locking gear for pushing due to the adjustable circulation gap of the adjusting section, so that the crushing, thinning and emulsifying of adipose tissues and fat cells are realized, and SVF cells can smoothly pass through the circulation gap and are not damaged. In addition, because the positioning function of the positioning component, the adjusting component can be fixed at the locking gear, so that the position of the circulation gap adjustment is more accurate. Therefore, the converter of the embodiment of the utility model can greatly improve the yield and the cell activity of SVF cells, so that the separation effect of the converter is excellent, and the accuracy in adjustment is higher.

In some embodiments, the positioning assembly is disposed on the housing, the positioning assembly being in abutment with the adjustment assembly.

In some embodiments, the plurality of locking gears are arranged at intervals along the moving direction of the adjusting assembly, the circulation gaps corresponding to different locking gears are different, and the adjusting assembly can be matched with the positioning assemblies at different positions to position the adjusting assembly on different locking gears.

In some embodiments, the positioning assembly comprises a plurality of positioning pieces, the positioning pieces are arranged at intervals along the direction of the adjusting assembly, the positioning pieces correspond to the locking gears one by one, and the adjusting assembly can elastically abut against the positioning pieces at different positions.

In some embodiments, the positioning assembly comprises a ball plunger, the ball plunger is arranged on the shell, a concave part is arranged on the adjusting assembly, the ball end of the ball plunger is elastically abutted with the concave part, and/or a closed cavity is formed in the shell, and the adjusting section is positioned in the closed cavity.

In some embodiments, the adjusting assembly includes a connecting member, a first extrusion block and a second extrusion block, the connecting member is connected with the housing and the first extrusion block, the adjusting section is disposed between the first extrusion block and the second extrusion block, the connecting member is movable relative to the housing so that the first extrusion block and the second extrusion block are close to and far away from each other, and the first extrusion block can abut against the positioning assembly to position the adjusting assembly to the locking gear.

In some embodiments, a concave part is arranged on the first extrusion block, and the positioning component is elastically abutted with the concave part; and/or the connecting piece is a threaded piece and is in threaded fit with the threaded piece and the shell; and/or, the first extrusion block is clamped with the connecting piece.

In some embodiments, the shell comprises two half shells, the two half shells are detachably connected, the connecting piece is connected with one of the two half shells, the second extrusion block is arranged on the other of the two half shells, the half shells are respectively provided with a first pipe groove and a second pipe groove along the length direction of the conversion pipe, the first pipe groove is abutted against the outer edge of the first end of the conversion pipe, and the second pipe groove is abutted against the outer edge of the second end of the conversion pipe; and/or the converter further comprises two first connectors, the two first connectors are respectively arranged at two ends of the conversion pipe and detachably connected with the injector, the half shell is respectively provided with a first matching groove and a second matching groove along the length direction of the conversion pipe, the first matching groove is abutted against the outer edge of one first connector, and the second matching groove is abutted against the outer edge of the other first connector; and/or one of the two half shells is provided with a positioning part, the other half shell is provided with a positioning groove, and the positioning part is matched in the positioning groove.

In some embodiments, the adjustment assembly is located outside the adjustment section, the adjustment section is resilient, and the adjustment assembly can press against an outer wall of the adjustment section to change the flow gap of the adjustment section.

A separation device according to another embodiment of the present utility model includes: the converter is the converter according to any one of the embodiments of the present utility model, and two injectors are respectively provided at two ends of the conversion tube.

According to the separating device provided by the embodiment of the utility model, the fat extract to be separated can be pushed through the conversion tube repeatedly through the two syringes, and the circulation gap of the adjusting section can be adjusted to the locking gear for pushing due to the adjustable circulation gap of the adjusting section, so that the crushing, thinning and emulsifying of adipose tissues and fat cells are realized, and SVF cells can smoothly pass through the circulation gap and are not damaged. In addition, because the positioning function of the positioning component, the adjusting component can be fixed at the locking gear, so that the position of the circulation gap adjustment is more accurate. Therefore, the separation device provided by the embodiment of the utility model can greatly improve the yield and the cell viability of SVF cells, so that the separation effect of the separation device is excellent, and the accuracy in adjustment is higher.

Drawings

Fig. 1 is a front view of a separation device according to an embodiment of the present utility model.

Fig. 2 is an isometric view of a separation device according to an embodiment of the utility model.

Fig. 3 is an exploded view of a transducer of an embodiment of the present utility model.

Fig. 4 is a cross-sectional view of a transducer of an embodiment of the present utility model.

Fig. 5 is a schematic view of a switching tube of a switch of an embodiment of the present utility model.

Fig. 6 is a schematic view of a positioning member of a transducer according to an embodiment of the present utility model.

Fig. 7 is a schematic view of a syringe of a separation device of an embodiment of the present utility model.

FIG. 8 is an enlarged schematic view of SVF cells isolated by a separation device according to an embodiment of the utility model under a microscope.

Reference numerals:

1. a converter;

11. a switching tube; 111. a regulating section;

12. an adjustment assembly; 121. a connecting piece; 1211. a clamping table; 122. a first extrusion block; 1221. a clamping groove; 1222. a recessed portion; 123. a second extrusion block;

13. a housing; 131. a half shell; 1311. a first mating groove; 1312. a second mating groove; 1313. a positioning groove; 1314. a positioning part; 1315. positioning holes;

14. a first joint;

15. a positioning assembly; 151. a positioning piece; 1511. a plunger body; 1512. an elastic member; 1513. ball head;

16. a bolt;

2. a syringe; 21. a syringe; 22. an injection rod; 23. a piston; 24. and a second joint.

Detailed Description

Reference will now be made in detail to embodiments of the present utility model, examples of which are illustrated in the accompanying drawings. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model.

A converter and a separation apparatus having the same according to an embodiment of the present utility model are described below with reference to fig. 1 to 8.

As shown in fig. 1 to 8, a converter 1 according to an embodiment of the present utility model includes: a transition tube 11, an adjustment assembly 12, a housing 13, and a positioning assembly 15. The transfer tube 11 is arranged through the housing 13, the transfer tube 11 having an adjustment section 111, the adjustment section 111 being located in the housing 13, the end of the transfer tube 11 being intended to be connected to the syringe 2. It will be appreciated that the adjustment section 111 is located between a first end of the transfer tube 11 (e.g. the left end of the transfer tube 11 in fig. 5) and a second end of the transfer tube 11 (e.g. the right end of the transfer tube 11 in fig. 5), the first end of the transfer tube 11 and the second end of the transfer tube 11 being connected to two syringes 2, respectively.

The adjustment assembly 12 is connected to the adjustment section 111, the adjustment assembly 12 being movable relative to the housing 13 to change the flow gap of the adjustment section 111. A positioning assembly 15 is provided on one of the housing 13 and the adjustment assembly 12, the positioning assembly 15 being abuttable against the other of the housing 13 and the adjustment assembly 12 to position the adjustment assembly 12 to the locking gear.

It will be appreciated that the cross-sectional flow area of the first end of the transition duct 11 and the cross-sectional flow area of the second end of the transition duct 11 are both smaller than the cross-sectional flow area of the conditioning section 111. The modulation component 12 may adjust the flow gap of the modulation segment 111 to be less than the adipocyte diameter (e.g., 200 um) and greater than the SVF cell population diameter (e.g., 2um to 70 um). The two injectors 2 may cyclically push the fat extract so that the fat extract may be repeatedly pushed through the adjustment section 111.

In addition, the positioning assembly 15 can lock the adjusting assembly 12, so that the problem that the adjusting assembly 12 is shifted when the converter 1 performs injection is avoided, and the adjusting precision of the converter 1 is improved.

According to the converter 1 of the embodiment of the utility model, the fat extract to be separated can be pushed through the conversion tube 11 repeatedly through the injector 2, and the circulation gap of the adjusting section 111 can be adjusted to the locking gear for pushing due to the adjustable circulation gap of the adjusting section 111, so that the crushing, thinning and emulsifying of the adipose tissue and the adipose cells can be realized, and the SVF cells can smoothly pass through the circulation gap without being damaged. In addition, due to the positioning function of the positioning component 15, the adjusting component 12 can be fixed in the locking gear, so that the position of the circulation gap adjustment is more accurate. Therefore, the converter 1 of the embodiment of the utility model can greatly improve the yield and the cell viability of SVF cells, so that the separation effect of the converter 1 is excellent, and the accuracy in adjustment is higher.

For example, the positioning assembly 15 is disposed on the housing 13, and the positioning assembly 15 abuts against the adjusting assembly 12 to fix the adjusting assembly 12 in the locking position.

For another example, the positioning assembly 15 is disposed on the adjusting assembly 12, and the positioning assembly 15 abuts against the housing 13 to fix the adjusting assembly 12 in the locking position.

In a specific example, as shown in fig. 3, the positioning assembly 15 is disposed on the housing 13, and the positioning assembly 15 elastically abuts against the adjusting assembly 12. It can be appreciated that, since the positioning assembly 15 is elastically abutted with the adjusting assembly 12, an operator can apply an external force to the adjusting assembly 12 to elastically offset and separate the positioning assembly 15 from the adjusting assembly 12, so that the risk of misoperation of the operator is reduced, and the use of the operator is facilitated.

In some embodiments, as shown in fig. 1 to 3, the locking gear is multiple, and the multiple locking gears are arranged at intervals along the moving direction of the adjusting component 12, and the circulation gaps corresponding to different locking gears are different, so that the adjusting component 12 can be matched with the positioning components 15 at different positions to position the adjusting component 12 to different locking gears, thereby further improving the adjusting precision of the converter 1.

It will be appreciated that in embodiments of the utility model, the housing 13 may be provided as a closed structure. The adjusting mechanism in the related art is designed as stepless screw adjustment. In clinical use, the internal structure of the transducer 1 is visible, and a doctor needs to visually correspond to the external scale and the position of the pressing block of the internal adjusting mechanism to realize the quantitative displacement of the adjusting mechanism. While the converter 1 of the embodiment of the present utility model may adopt a totally enclosed structure. Optionally, the housing 13 has a closed chamber therein, and the adjustment section 111 is located within the closed chamber.

In order to guarantee the blind operation feasibility of the closed structure, the fixing and mechanical feedback of different gears can be realized by arranging the positioning assembly 15, so that on one hand, the difficulty of accurate operation and the risk of incorrect operation of the traditional electrodeless adjustment are reduced, and on the other hand, the design of the fully-closed internal structure is beneficial to the physical protection of the internal structure of the product, and the risk of exposing contents due to incorrect operation is reduced.

In an embodiment of the utility model, the converter 1 employs a multi-gear adjustment. For example, the flow gap of the adjustment section 111 is different for each stage of lock gear. It will be appreciated that during initial pushing, a locking gear with a larger flow gap may be selected, and then the distance to the flow gap is sequentially reduced to increase the yield and viability of SVF cells.

Alternatively, as shown in fig. 3, the positioning assembly 15 includes a plurality of positioning members 151, the plurality of positioning members 151 are arranged at intervals along the direction of the adjusting assembly 12, the plurality of positioning members 151 are in one-to-one correspondence with the plurality of locking gears, and the adjusting assembly 12 can elastically abut against the positioning members 151 at different positions. For example, the housing 13 is provided with a positioning hole 1315, and the positioning member 151 is detachably mounted on the positioning hole 1315, thereby facilitating the mounting and dismounting of the positioning assembly 15.

For example, as shown in fig. 3 and 6, the positioning member 151 is a ball plunger provided on the housing 13, and the assembly position of the ball plunger is determined by the gear design and the flow gap of the adjustment section 111. The adjusting component 12 is provided with a recess 1222, and the ball end of the ball plunger elastically abuts against the recess 1222. Specifically, the ball plunger includes a plunger body 1511, an elastic member 1512, and a ball 1513, the plunger body 1511 is screw-fitted in the positioning hole 1315, the ball 1513 is provided at an end of the plunger body 1511, and the elastic member 1512 is provided between the ball 1513 and the plunger body 1511. The resilient member 1512 may be a spring, and the spring urges the ball 1513 toward the adjustment assembly 12.

When the operator switches between different locking gears, the adjusting assembly 12 can be driven to move relative to the housing 13, and when the external force applied to the adjusting assembly 12 exceeds the elastic force between the ball 1513 and the recess 1222, the adjusting assembly 12 can go beyond the locking gear and continue to move to the next locking gear.

In some embodiments, as shown in fig. 3 and 4, the adjustment assembly 12 includes a connector 121, a first extrusion block 122, and a second extrusion block 123, the connector 121 being connected to the housing 13 and the first extrusion block 122, the adjustment segment 111 being disposed between the first extrusion block 122 and the second extrusion block 123, the connector 121 being movable relative to the housing 13 to move the first extrusion block 122 and the second extrusion block 123 toward and away from each other, the first extrusion block 122 being movable against the positioning assembly 15 to position the adjustment assembly 12 in the locked gear. It is understood that the connecting member 121 may drive the first extrusion 122 to move in a direction toward or away from the second extrusion 123 such that the first extrusion 122 and the second extrusion 123 compress the adjustment segment 111. The first extrusion block 122 is provided with a recess 1222, and when the first extrusion block 122 moves, the recess 1222 on the first extrusion block 122 can be elastically clamped with ball plungers at different positions.

Alternatively, as shown in fig. 3 and 4, the connecting member 121 is screw-fitted with the housing 13. It will be appreciated that the housing 13 is provided with a threaded hole into which the connector 121 is screwed, and that the connector 121 may be screwed in a direction towards the adjustment section 111 such that the connector 121 presses the adjustment section 111, thereby reducing the distance of the flow gap. Likewise, the connecting member 121 may be threaded in a direction away from the adjustment section 111 such that the connecting member 121 is spaced away from the adjustment section 111, thereby increasing the distance of the flow gap.

Alternatively, as shown in fig. 3 and 4, the connecting piece 121 is provided with a clamping table 1211, the first extrusion block 122 is provided with a clamping groove 1221, and the clamping table 1211 is elastically clamped in the clamping groove 1221, so that the fixing of the connecting piece 121 and the first extrusion block 122 is completed, and the connecting piece is convenient to install and simple to process and manufacture.

Optionally, a guide slideway (not shown) is provided in the housing 13, and the first extrusion block 122 is matched with the guide slideway, so that the first extrusion block 122 only slides along the length direction of the guide slideway, thereby improving the accuracy of adjustment of the adjustment assembly 12.

In some embodiments, as shown in fig. 3 and 4, the housing 13 includes two half-shells 131, the two half-shells 131 are detachably connected, the connecting member 121 is connected to one of the two half-shells 131, and the second extrusion block 123 is provided on the other of the two half-shells 131. For example, the two half-shells 131 are connected by bolts 16, thereby facilitating the mounting and dismounting of the housing 13. When the conversion tube 11 needs to be installed, the two half-shells 131 may be separated, then the conversion tube 11 is installed between the two half-shells 131, and then the two half-shells 131 are fixed by the bolts 16, thereby completing the installation work of the converter 1.

For example, as shown in fig. 3, the half shell 131 is provided with a first pipe groove (not shown) and a second pipe groove (not shown) along the length direction of the converting pipe 11, the first pipe groove is abutted against the outer edge of the first end of the converting pipe 11, and the second pipe groove is abutted against the outer edge of the second end of the converting pipe 11, and it can be understood that the first pipe groove and the second pipe groove are both semicircular hole structures. Taking the first pipe groove as an example, the first pipe grooves of the two half-shells 131 can be spliced into a whole round hole, so that the end part of the conversion pipe 11 is fixed, the conversion pipe 11 is prevented from moving relative to the shell 13, and the installation reliability of the conversion pipe 11 is improved.

Further, as shown in fig. 3, the converter 1 includes two first connectors 14, the two first connectors 14 are respectively disposed at two ends of the conversion tube 11 and detachably connected with the syringe 2, the half-shell 131 is respectively provided with a first mating groove 1311 and a second mating groove 1312 along the length direction of the conversion tube 11, the first mating groove 1311 abuts against an outer edge of one of the first connectors 14, and the second mating groove 1312 abuts against an outer edge of the other first connector 14. For example, the first fitting 14 is a luer fitting. The first fitting groove 1311 and the second fitting groove 1312 are both half quincuncial hole structures, so that the first connector 14 can be compressed, and the installation firmness of the first connector 14 can be improved.

Alternatively, as shown in fig. 3, one of the two half-shells 131 is provided with a positioning portion 1314, and the other half-shell 131 is provided with a positioning groove 1313, and the positioning portion 1314 is fitted in the positioning groove 1313, and it is understood that the positioning portion 1314 may be installed in the positioning groove 1313 when the half-shells 131 are installed, so that the housing 13 is pre-fixed, so that a screw member smoothly passes through the two half-shells 131, and the convenience of installing the housing 13 is improved.

Optionally, the half shell 131 may be made of medical stainless steel (1 Cr18Ni 9T), 316L stainless steel, 317L stainless steel, TC4 titanium alloy, medical plastic (PC, PP, ABS), and the like.

The material of the first joint 14 may be medical stainless steel (1 Cr18Ni9T, 316L or 317L), TC4 titanium alloy, medical plastic (PC, PP, ABS), or the like.

The connecting piece 121, the first extrusion block 122 and the second extrusion block 123 may be made of medical stainless steel (1 Cr18Ni 9T), 316L stainless steel, 317L stainless steel, TC4 titanium alloy, etc.

In some embodiments, as shown in fig. 5, where the adjustment assembly 12 is located outside the adjustment section 111, the adjustment section 111 is resilient and the adjustment assembly 12 can press against the outer wall of the adjustment section 111 to change the flow gap of the adjustment section 111. It will be appreciated that the adjustment assembly 12 is mounted externally of the adjustment section 111, the adjustment assembly 12 varying the size of the flow gap by squeezing the adjustment section 111. For example, the conversion tube 11 is a silicone tube, and the conversion tube 11 can be elastically deformed by external force. Because the conversion tube 11 is a silica gel hose, the damage to SVF cells during the pushing of the fat extract can be further avoided, the yield and the activity of the SVF cells are improved, and the device has the advantages of simple structure, convenient operation and convenient processing and manufacturing.

As shown in fig. 1 and 2, a separation apparatus according to another embodiment of the present utility model includes: a transducer 1 and two syringes 2, the transducer 1 is the transducer 1 of the present utility model, and the two syringes 2 are respectively arranged at two ends of the transducer tube 11.

According to the separating device provided by the embodiment of the utility model, the fat extract to be separated can be pushed through the two injectors 2 repeatedly passing through the conversion pipe 11, and the circulation gap of the adjusting section 111 can be adjusted to the locking gear for pushing due to the adjustable circulation gap of the adjusting section 111, so that the crushing, thinning and emulsifying of the fat tissues and the fat cells are realized, and the SVF cells can smoothly pass through the circulation gap without being damaged. In addition, due to the positioning function of the positioning component 15, the adjusting component 12 can be fixed in the locking gear, so that the position of the circulation gap adjustment is more accurate. Therefore, the separation device provided by the embodiment of the utility model can greatly improve the yield and the cell viability of SVF cells, so that the separation effect of the separation device is excellent, and the accuracy in adjustment is higher.

Alternatively, as shown in fig. 7, the syringe 2 includes a syringe barrel 21, a syringe rod 22, and a piston 23, and an end of the syringe barrel 21 is detachably connected to an end of the transfer tube 11, for example, an end of the syringe barrel 21 is fitted with a second connector 24, and the second connector 24 is a luer connector. The piston 23 is slidably disposed within the syringe 21, and the injection rod 22 is detachably connected to the piston 23. For example, injection rod 22 is threadably connected to piston 23. It will be appreciated that the syringe 21 and the piston 23 may constitute a centrifugal separator, since the injection rod 22 is detachably connected to the piston 23. That is, after the syringe 21 removes the injection rod 22, a stopper may be provided at the end of the syringe 21, and then the syringe 21 may be transferred to a centrifuge for separation.

In a preferred embodiment, the injector 2 is a centrifugal separator. It will be appreciated that the injector 2 of the present embodiment may be used as an injection device or as a centrifugal separator, thereby simplifying the operation steps of the SVF production method and providing higher SVF yield and activity.

Specifically, the method for preparing SVF by using the separation device of the utility model comprises the following steps:

the subject's abdominal superficial fat, 100ml of fat extract, was extracted.

Placing the fat extract in a centrifugal separator for centrifugal separation, wherein the centrifugal force is 700×g, the centrifugal time is 1min, the fat extract is centrifuged into three layers, the uppermost layer is a fat layer, and the lowermost layer is a bloodred swelling liquid and a blood water layer.

The injection rod 22 of the injector 2 is screwed on, the bottom swelling liquid and the blood water layer are slowly and stably pushed out downwards, the injector 2 of the separating device is used for extracting the fat extract cell layer, then the injector 2 and the converter 1 are screwed together, the connecting piece 121 is continuously rotated so as to adjust the circulation gap of the adjusting section 111, when the first click sound is heard and obvious rotation resistance is felt, the adjusting section 111 is positioned at the first locking gear, the circulation gap of the adjusting section 111 is 5mm at the moment, the injector 2 is repeatedly pushed and injected under the locking gear, the fat extract is emulsified, and the pushing and injecting times are more than or equal to 10 times;

then the connecting piece is rotated forcefully to enable the pressing block to continuously move downwards beyond the first locking gear, when a second sound click sound is heard and obvious rotation resistance can be felt, the adjusting section 111 is positioned at the second locking gear, the circulation gap of the adjusting section 111 is 2mm, the injector 2 is repeatedly pushed and injected under the locking gear, the fat extract is emulsified, and the pushing and injection times are more than or equal to 10 times;

then the connecting piece is rotated forcefully to enable the pressing block to continuously move downwards beyond the second locking gear, when a third click sound is heard and obvious rotation resistance can be felt, the adjusting section 111 is positioned at the third locking gear, at the moment, the circulation gap of the adjusting section 111 is 5 mu m, and the injector 2 is repeatedly pushed and injected in the gear, so that the fat extract is emulsified, and the pushing and injection times are preferably 20 times;

injecting the emulsified fat into a centrifugal separator for secondary centrifugal separation, wherein the centrifugal force is 2060 Xg, the centrifugal time is 15min, the fat extract is centrifuged into three layers, the upper layer is a fat layer, the middle layer is a fat layer containing part of SVF cells, fragments and fat droplets (the bottom of the middle layer is a milky light layer), and the lowest layer is a SVF layer.

The SVF suspension layer and the upper milky white layer were drawn up by syringe 2 connection at 1:1 into proper amount of physiological saline for injection. Placing the mixture into a centrifugal separator, shaking thoroughly, and selecting 700X centrifugal forceg, centrifugation time was chosen to be 1min. After centrifugation, the extract was separated into two layers, the lower liquid being a SVF cell suspension. As shown in FIG. 8, the cell morphology was observed under a microscope and the count result was 3X 10 ⁷ Cell viability was calculated to be > 90% by trypan blue staining per ml. Therefore, the separation device provided by the embodiment of the utility model can greatly improve the yield and the activity of SVF cells, and has excellent separation effect.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present utility model, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.

In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present utility model, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

For purposes of this disclosure, the terms "one embodiment," "some embodiments," "example," "a particular example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.

While the above embodiments have been shown and described, it should be understood that the above embodiments are illustrative and not to be construed as limiting the utility model, and that variations, modifications, alternatives, and variations of the above embodiments may be made by those of ordinary skill in the art without departing from the scope of the utility model.

Claims

1. A converter, comprising:

a housing;

the conversion tube penetrates through the shell and is provided with an adjusting section, the adjusting section is positioned in the shell, and the end part of the conversion tube is connected with the injector;

the adjusting assembly is connected with the adjusting section and can move relative to the shell so as to change the circulation gap of the adjusting section;

the positioning component is arranged on one of the shell and the adjusting component, and can be propped against the other of the shell and the adjusting component to position the adjusting component to a locking gear.

2. The transducer of claim 1, wherein the positioning assembly is disposed on the housing, the positioning assembly being in abutment with the adjustment assembly.

3. The converter according to claim 2, wherein a plurality of said locking gear steps are provided, said plurality of said locking gear steps being arranged at intervals along the direction of movement of said adjustment assembly, said flow-through gaps corresponding to different ones of said locking gear steps being different, said adjustment assembly being cooperable with said positioning assemblies in different positions for positioning said adjustment assembly onto different ones of said locking gear steps.

4. A transducer according to claim 3, wherein the positioning assembly comprises a plurality of positioning members arranged at intervals along the direction of the adjustment assembly, the plurality of positioning members are in one-to-one correspondence with the plurality of locking gears, and the adjustment assembly is elastically abutted with the positioning members at different positions.

5. The transducer of claim 1, wherein the positioning assembly comprises a ball plunger disposed on the housing, the adjustment assembly having a recess, a ball end of the ball plunger resiliently abutting the recess;

and/or, a closed cavity is formed in the shell, and the adjusting section is positioned in the closed cavity.

6. The transducer of any of claims 1-5, wherein the adjustment assembly includes a connector, a first squeeze block and a second squeeze block, the connector being coupled to the housing and the first squeeze block, the adjustment section being disposed between the first squeeze block and the second squeeze block, the connector being movable relative to the housing to move the first squeeze block and the second squeeze block toward and away from each other, the first squeeze block being abuttable against the positioning assembly to position the adjustment assembly to the locked gear.

7. The transducer of claim 6, wherein the first extrusion block has a recess, the positioning assembly resiliently abutting the recess;

and/or the connecting piece is a threaded piece and is in threaded fit with the threaded piece and the shell;

and/or, the first extrusion block is clamped with the connecting piece.

8. The transducer of claim 6, wherein the housing comprises two half-shells, the two half-shells being detachably connected, the connector being connected to one of the two half-shells, the second extrusion being provided on the other of the two half-shells,

the half shell is provided with a first pipe groove and a second pipe groove along the length direction of the conversion pipe respectively, the first pipe groove is abutted against the outer edge of the first end of the conversion pipe, and the second pipe groove is abutted against the outer edge of the second end of the conversion pipe;

and/or the converter further comprises two first connectors, the two first connectors are respectively arranged at two ends of the conversion pipe and detachably connected with the injector, the half shell is respectively provided with a first matching groove and a second matching groove along the length direction of the conversion pipe, the first matching groove is abutted against the outer edge of one first connector, and the second matching groove is abutted against the outer edge of the other first connector;

and/or one of the two half shells is provided with a positioning part, the other half shell is provided with a positioning groove, and the positioning part is matched in the positioning groove.

9. The transducer of claim 1, wherein the conditioning assembly is located outside the conditioning section, the conditioning section having resiliency, the conditioning assembly being compressible against an outer wall of the conditioning section to change a flow gap of the conditioning section.

10. A separation device, comprising:

a converter according to any one of claims 1-9;

the two injectors are respectively arranged at the two ends of the conversion tube.