AU2013312546B2

AU2013312546B2 - Centrifuge tube structure

Info

Publication number: AU2013312546B2
Application number: AU2013312546A
Authority: AU
Inventors: Chaocheng YANG
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-09-10
Filing date: 2013-09-04
Publication date: 2016-12-22
Anticipated expiration: 2033-09-04
Also published as: WO2014036945A1; AU2013312546A1; CN103657756A

Abstract

Disclosed is a centrifuge tube structure. The centrifuge tube structure comprises a tube body (2), a first blocking body (3), and a second blocking body (4). The tube body (2) has a first receiving trough (20), a second receiving trough (22), and a necking portion (21). The necking portion (21) is connected to the first receiving trough (20) and the second receiving trough (22). The first blocking body (3) is fixed on the first receiving trough (20), and defines a fixed volume compartment (200) with the first receiving trough (20) commonly. The second blocking body (4) moves along the second receiving trough (22), and defines a variable volume compartment (220) with the second receiving trough (22) commonly. The necking portion (21) defines a fluid communication path (210) to communicate the fixed volume compartment (200) with the variable volume compartment (220).

Description

ι 2013312546 09 Apr 2015

CENTRIFUGAL TUBE STRUCTURE FIELD OF THE INVENTION

[0001] The present invention relates to a centrifugal tube structure, and more particularly to a centrifugal tube structure for facilitating extracting a thin layer of solution part.

BACKGROUND OF THE INVENTION

[0002] With increasing development of the biomedical technology, the researches on human blood become more complete for the biomedical field. The various ingredients in the blood have their important functions. If the ingredients in the blood can be separated smoothly and used for adjuvant therapy in medical treatment, the therapeutic efficacy can be largely enhanced. In accordance with a usual method of separating the ingredients of the blood, a test tube containing the blood is placed in a centrifuge device and then the centrifuge device starts. By a centrifuging action, the blood in the test tube is fractionated into plural component parts.

[0003] As mentioned above, after the test tube is centrifuged by the centrifuge device, the blood in the test tube is fractionated into an upper solution part, a middle solution part and a lower solution part. The upper solution part mainly contains plasma. The lower solution part mainly contains erythrocytes. The middle solution part is called as a buffy coat layer consisting of platelets and lymphocytes, leukocytes and stem cells. Due to the medical and commercial value, the buffy coat layer is extremely valuable. However, since the buffy coat layer in the test tube is a thin layer and the thickness of the buffy coat layer is very small, it is difficult to extract the buffy coat layer from the ordinary test tube. If the extraction is not carefully done, the upper solution part or the lower solution part is easily extracted. In other words, it is difficult for the operator to extract the buffy coat layer [0004] For example, a bio device is disclosed in Taiwanese Patent Publication No. 201124191, which is entitled “Bio device for extracting hematopoietic stem cells and mesenchymal stem cells within peripheral blood”. The bio device comprises a main body 11 and a top cover 10. The main body 11 comprises a neck part 110. The top cover 10 is detachably moved forth or back in the longitudinal direction of the main body 11. Moreover, when the top cover 10 is rotated by the operator, the top cover 10 may be

6357970_1 (GHMatters) P99405.AU NIOUSHAA 2 2013312546 09 Apr 2015 moved forth or back in the longitudinal direction of the main body 11. However, it is found that the movable mechanism of the top cover 10 of the bio device is not necessary. If the top cover 10 is modified as a fixed cover or the top cover 10 is integrally formed with the main body 11, the structure and process ease of the structure will be largely increased. Moreover, if the top cover 10 is changed from the movable cover to the fixed cover on the main body 11, the bio device becomes a single-use disposable device. However, for saving cost, the unscrupulous vendor may open the top cover 10 and clean the bio device in order to repeatedly use the bio device. The bio device with the fixed cover can solve the above drawbacks.

[0005] Moreover, the bio device further comprises a bottom cover 13. When the top cover 10 is rotated by the operator, the bottom cover 13 may be moved forth or back in the longitudinal direction of the main body 11. The bio device further comprises a sealing part 12 for sealing a lower reservoir 120 of the main body 11. When the bottom cover 13 is rotated by the operator, the sealing part 12 is correspondingly elevated, and thus the volume of the lower reservoir 120 is decreased. However, since the bottom cover is twisted by the fingers of the operator, some problems may occur. For example, a component of the twist force is perpendicular to the longitudinal direction of the main body 11. Consequently, the whole bio device has a tendency to swinging toward left and right sides. Under this circumstance, the upper solution part, the middle solution part and the lower solution part which have been clearly fractionated are possibly mixed with each other again.

[0006] Therefore, there is a need of providing an improved centrifugal tube structure for easily extracting the fractionated solution. Moreover, the centrifugal tube structure is easily fabricated because of the simplified structure, and can ensure the single-use purpose. Moreover, while the volume of the lower reservoir is decreased, the force applied to the centrifugal tube structure is substantially in the longitudinal direction. Consequently, the possibility of swinging the fractionated solution within the centrifugal tube structure will be minimized.

SUMMARY OF THE INVENTION

[0007] A possible advantage of the present invention is the provision of a centrifugal tube having a simplified structure, the centrifugal tube structure is easily fabricated and facilitates mass production.

6357970_1 (GHMatters) P99405.AU NIOUSHAA 3 2013312546 29 Nov 2016 [0008] Another possible advantage is the provision of a centrifugal tube structure having a mechanism for receiving an axial force. Consequently, while the fractionated solution within the centrifugal tube structure is pulled up, the possibility of swinging the centrifugal tube structure will be minimized. Due to the centrifugal tube structure, the operator can extract the fractionated solution part more easily.

[0009] In an embodiment, the present invention provides a centrifugal tube structure. The centrifugal tube structure includes a tube body, a first blocking body and a second blocking body. The tube body includes a first reservoir, a second reservoir and a neck part connected with the first reservoir and the second reservoir. The first blocking body is fixed on the first reservoir, wherein a fixed volume compartment is defined by the first blocking body and the first reservoir collaboratively. The second blocking body includes a bottom cover and a piston part. The bottom cover is fixed on a bottom end of the second reservoir. The second reservoir is sealed by the piston part. The piston part is accommodated within the second reservoir. A variable volume compartment is defined by the piston part and the second reservoir collaboratively. Moreover, a communication channel is defined by the neck part, and the communication channel is in communication with the fixed volume compartment and the variable volume compartment.

[0010] In an embodiment, the present invention provides a centrifugal tube structure, comprising: a tube body comprising a first reservoir, a second reservoir and a neck part connected with the first reservoir and the second reservoir; a first blocking body fixed on the first reservoir, wherein a fixed volume compartment is defined by the first blocking body and the first reservoir collaboratively; and a second blocking body comprising a bottom cover movably disposed at a bottom of the tube body and a piston part arranged between the neck part and the bottom cover and movable with a push-pull force toward or away from the neck part, wherein a variable volume compartment is defined by the piston part and the second reservoir collaboratively, and the bottom cover serves as a stopper for limiting the piston part from being over-pulled out of the second reservoir,

8460732_1 (GHMatters) P99405.AU 3a 2013312546 29 Nov 2016 wherein a communication channel is defined by the neck part, and the communication channel is in communication with the fixed volume compartment and the variable volume compartment.

[0011] Preferably, the first reservoir has a first opening part, and the first blocking body is fixed on the first opening part of the first reservoir.

[0012] Preferably, the first blocking body and the first reservoir are integrally formed with each other.

[0013] Preferably, the first blocking body has a vent, and the vent is aligned with the neck part, wherein the fixed volume compartment is in communication with surroundings through the vent.

[0014] Preferably, the first blocking body has a liquid inlet, and the liquid inlet is located beside the vent.

[0015] Preferably, the first blocking body includes two plugs, and the liquid inlet and the vent are sealed by the two plugs, respectively, wherein the plug that seals the vent has a perforation, and a syringe needle is permitted to be introduced into the first reservoir through the perforation of the plug.

8460732J (GHMallers) P99405.AU 4 2013312546 09 Apr 2015 [0015] Preferably, a cross section area of the neck part is smaller than a cross section area of the first reservoir and a cross section area of the second reservoir.

[0016] Preferably, the piston part is movable within the second reservoir, so that the variable volume compartment is defined by the piston part and the second reservoir collaboratively; or the piston part is accommodated within the second reservoir and an external force is applied to the piston part to result in deformation of the piston part, so that the variable volume compartment is defined by the piston part and the second reservoir collaboratively.

[0017] Preferably, the second blocking body further comprises a push rod, a center of the bottom cover is a hollow part, the push rod is penetrated through the hollow part and connected with the piston part, and the piston part is pushed toward the neck part by the push rod when an external force is applied to the push rod.

[0018] The present invention further provides a centrifugal tube structure. The centrifugal tube structure includes a tube body, a first blocking body and a second blocking body. The tube body includes a first reservoir, a second reservoir and a neck part connected with the first reservoir and the second reservoir. The first blocking body is fixed on the first reservoir, wherein a fixed volume compartment is defined by the first blocking body and the first reservoir collaboratively. A variable volume compartment is defined by the second blocking body and the second reservoir collaboratively. Moreover, a communication channel is defined by the neck part, and the communication channel is in communication with the fixed volume compartment and the variable volume compartment.

[0019] Preferably, the first blocking body and the first reservoir are integrally formed with each other.

[0020] Preferably, the first reservoir has a first opening part, and the first blocking body is fixed on the first opening part of the first reservoir.

[0021] Preferably, the first blocking body has a vent, and the vent is aligned with the neck part, wherein the fixed volume compartment is in communication with surroundings through the vent.

[0022] Preferably, the first blocking body further has a liquid inlet, and the liquid inlet is located beside the vent.

6357970_1 (GHMatters) P99405.AU NIOUSHAA 5 2013312546 09 Apr 2015 [0023] Preferably, the first blocking body further includes two plugs, and the liquid inlet and the vent are sealed by the two plugs, respectively, wherein the plug that seals the vent has a perforation, and a syringe needle is permitted to be introduced into the first reservoir through the perforation of the plug.

[0024] Preferably, a cross section area of the neck part is smaller than a cross section area of the first reservoir and a cross section area of the second reservoir.

[0025] Preferably, the second blocking body includes a piston part, and the piston part is accommodated within the second reservoir and movable within the second reservoir, so that the variable volume compartment is defined by the piston part and the second reservoir collaboratively; or the second blocking body includes a piston part, the piston part is accommodated within the second reservoir, and an external force is applied to the piston part to result in deformation of the piston part, so that the variable volume compartment is defined by the piston part and the second reservoir collaboratively.

[0026] Preferably, the second blocking body further includes a bottom cover, and the bottom cover is fixed on a bottom end of the second reservoir, so that the piston part is arranged between the bottom cover and the neck part.

[0027] Preferably, the bottom cover is movably disposed within the second reservoir, wherein when the bottom cover is moved toward the neck part, the piston part is correspondingly pushed by the bottom cover, so that the piston part is moved toward the neck part.

[0028] Preferably, a surface of the bottom cover has male threads, a surface of the second reservoir has female threads, the male threads match the female threads, and the bottom cover is moved in an axial direction when the bottom cover is rotated about the axial direction.

[0029] Preferably, the bottom cover has a convex structure, the second reservoir has a concave structure, the convex structure matches the concave structure, so that the bottom cover is slidable relative to the second reservoir.

[0030] Preferably, the bottom cover is fixed on the second reservoir, and a center of the bottom cover is a hollow part.

[0031] Preferably, the second blocking body further includes a push rod, the push rod is penetrated through the hollow part and connected with the piston part, and the piston

6357970_1 (GHMatters) P99405.AU NIOUSHAA 6 2013312546 09 Apr 2015 part is pushed toward the neck part by the push rod when an external force is applied to the push rod.

[0032] Preferably, the second blocking body further includes a push rod, the push rod is connected with the piston part, and the piston part is pushed toward the neck part by the push rod when an external force is applied to the push rod.

[0033] The present invention further provides a centrifugal tube structure. The centrifugal tube structure includes a tube body, a fixing cover and a bottom cover. The tube body includes a first reservoir, a second reservoir and a neck part connected with the first reservoir and the second reservoir, wherein the first reservoir has a fixing means. The fixing cover is engaged with the fixing means of the first reservoir, wherein the fixing cover is fixed on the first reservoir through the fixing means. The bottom cover is located at a bottom end of the second reservoir.

[0034] Preferably, the fixing cover has a vent and a liquid inlet, and the vent is aligned with the neck part, wherein the fixed volume compartment is in communication with surroundings through the vent. After a syringe is penetrated through the liquid inlet, a portion of the syringe is introduced into the neck part.

[0035] Preferably, the centrifugal tube structure further includes a piston part, and the piston part is accommodated within the second reservoir and movable within the second reservoir.

[0036] Preferably, the second reservoir has a moving means, and the bottom cover is abutted against the moving means, so that the bottom cover is movably disposed within the second reservoir.

[0037] Preferably, the piston part is arranged between the bottom cover and the neck part, wherein when the bottom cover is moved toward the neck part, the piston part is correspondingly pushed by the bottom cover, so that the piston part is moved toward the neck part.

[0038] Preferably, the moving means of the second reservoir includes female threads of the second reservoir, a surface of the bottom cover has male threads, the male threads match the female threads, and the bottom cover is moved in an axial direction when the bottom cover is rotated about the axial direction.

6357970.1 (GHMatters) P99405.AU NIOUSHAA 7 2013312546 09 Apr 2015 [0039] Preferably, the moving means of the second reservoir includes a concave structure of the second reservoir, the bottom cover has a convex structure, and the convex structure matches the concave structure, so that the bottom cover is slidable relative to the second reservoir.

[0040] Preferably, the second blocking body further includes a push rod, the push rod is connected with the piston part, and the piston part is pushed toward the neck part by the push rod when an external force is applied to the push rod.

[0041] The present invention further includes a centrifugal tube structure. The centrifugal tube structure includes a tube body, a first blocking body and a second blocking body. The tube body includes a first reservoir, a second reservoir and a neck part connected with the first reservoir and the second reservoir. The first blocking body is fixed and positioned on a top edge of a reservoir body of the first reservoir. The second blocking body is movably coupled to a bottom edge of a reservoir body of the second reservoir. A volume compartment is defined by the first blocking body, the first reservoir, the neck part, the second reservoir and the second blocking body collaboratively. By moving the second blocking body, a volume distribution status of the volume compartment is correspondingly changed.

[0042] Preferably, the second blocking body further includes a piston part, and the piston part is disposed within the second reservoir and movable within the second reservoir.

[0043] Preferably, the second blocking body further includes a push rod, and the push rod is connected with the piston part. When an axial pushing force is applied to the push rod, the piston part is pushed by the push rod in a longitudinal direction and the piston part is linearly moved toward the neck part, so that the volume compartment is decreased. When an axial pulling force is applied to the push rod, the piston part is pulled by the push rod in a longitudinal direction and the piston part is linearly moved away from the neck part, so that the volume compartment is increased.

[0044] Preferably, an end of the push rod is connected with the piston part and integrally formed with the piston part; or an end of the push rod is hooked on the piston part.

[0045] Preferably, the second blocking body further includes a bottom cover, and the bottom cover is movably located at the bottom edge of the reservoir body of the second

6357970_1 (GHMatters) P99405.AU NIOUSHAA 8 2013312546 09 Apr 2015 reservoir, wherein when the bottom cover is moved toward the neck part, the piston part is correspondingly pushed by the bottom cover and the piston part is moved toward the neck part, so that the volume compartment is decreased; or when the bottom cover is moved away from the neck part, the piston part is correspondingly pulled by the bottom cover and the piston part is moved away from the neck part, so that the volume compartment is increased.

[0046] The present invention further includes a centrifugal tube structure. The centrifugal tube structure includes a tube body, a first blocking body and a second blocking body. The tube body includes a first reservoir, a second reservoir and a neck part connected with the first reservoir and the second reservoir. The first blocking body closes the first reservoir. The second blocking body includes a bottom cover and a piston part. The bottom cover is fixed on a bottom edge of the second reservoir. The second reservoir is sealed by the piston part. The piston part is movable within the second reservoir.

[0047] Preferably, the centrifugal tube structure further includes a push rod, and the push rod is connected with the piston part, wherein when an axial pushing force is applied to the push rod, the piston part is pushed by the push rod in a longitudinal direction and the piston part is linearly moved toward the neck part.

[0048] Preferably, a center of the bottom cover has a center hollow part.

[0049] Preferably, the centrifugal tube structure further includes a push rod, and the push rod is penetrated through the hollow part and connected with the piston part, wherein when an axial pushing force is applied to the push rod, the piston part is pushed by the push rod in a longitudinal direction and the piston part is linearly moved toward the neck part.

[0050] Preferably, the first blocking body is fixed on the first reservoir, so that a fixed volume compartment is defined by the first blocking body and the first reservoir collaboratively; or the first blocking body is movably disposed within the first reservoir, so that a variable volume compartment is defined by the first blocking body and the first reservoir collaboratively.

[0051] Preferably, the first blocking body has a vent, and the vent is aligned with the neck part, wherein the fixed volume compartment is in communication with surroundings through the vent.

6357970_1 (GHMatters) P99405.AU NIOUSHAA 9 2013312546 09 Apr 2015 [0052] Preferably, the first blocking body further has a liquid inlet, and the liquid inlet is located beside the vent.

[0053] Preferably, the first blocking body further includes two plugs, and the liquid inlet and the vent are sealed by the two plugs, respectively, wherein the plug that seals the vent has a perforation, and a syringe needle is permitted to be introduced into the first reservoir through the perforation of the plug.

[0054] Preferably, a cross section area of the neck part is smaller than a cross section area of the first reservoir and a cross section area of the second reservoir.

[0055] The present invention further includes a centrifugal tube structure. The centrifugal tube structure includes a tube body, a first blocking body, a piston part and a push rod. The tube body includes a first reservoir, a second reservoir and a neck part connected with the first reservoir and the second reservoir. The first blocking body closes the first reservoir. The piston part seals the second reservoir, wherein the piston part is movable within the second reservoir. The push rod is connected with the piston part. When an external force is applied to the push rod, the piston part is pushed by the push rod in a longitudinal direction and the piston part is linearly moved toward the neck part.

[0056] Preferably, the external force is an axial pushing force, so that the piston part is pushed by the push rod in a longitudinal direction and the piston part is linearly moved toward the neck part.

[0057] Preferably, the centrifugal tube structure further includes a bottom cover, and the bottom cover is fixed on a bottom end of the second reservoir, so that the piston part is arranged between the bottom cover and the neck part.

[0058] Preferably, the first blocking body is fixed on the first reservoir, so that a fixed volume compartment is defined by the first blocking body and the first reservoir collaboratively; or the first blocking body is movably disposed within the first reservoir, so that an additional variable volume compartment is defined by the first blocking body and the first reservoir collaboratively.

[0059] Preferably, the first blocking body has a vent, and the vent is aligned with the neck part, wherein the fixed volume compartment is in communication with surroundings through the vent.

6357970_1 (GHMatters) P99405.AU NIOUSHAA ίο 2013312546 09 Apr 2015 [0060] The present invention further includes a centrifugal tube structure. The centrifugal tube structure includes a tube body, a first blocking body and a movable member. The tube body includes first reservoir, a second reservoir and a neck part connected with the first reservoir and the second reservoir. The first blocking body closes the first reservoir. The movable member seals the second reservoir. When an axial pushing force is applied to the movable member, the movable member is linearly moved toward the neck part in a longitudinal direction.

[0061] Preferably, the movable member includes a piston part, wherein a variable volume compartment is defined by the piston part and the second reservoir collaboratively.

[0062] Preferably, the movable member further includes a push rod, and the push rod is connected with the piston part, wherein when the axial pushing force is applied to the push rod, the piston part is pushed by the push rod in the longitudinal direction and the piston part is linearly moved toward the neck part, so that the variable volume compartment is decreased.

[0063] Preferably, the first blocking body is fixed on the first reservoir, so that a fixed volume compartment is defined by the first blocking body and the first reservoir collaboratively; or the first blocking body is movably disposed within the first reservoir, so that an additional variable volume compartment is defined by the first blocking body and the first reservoir collaboratively.

[0064] Preferably, the first blocking body has a vent, and the vent is aligned with the neck part, wherein the fixed volume compartment or the additional variable volume compartment is in communication with surroundings through the vent.

[0065] The present invention further includes a centrifugal tube structure. The centrifugal tube structure includes a tube body, a first blocking body and a movable member. The tube body includes a first reservoir, a second reservoir and a neck part connected with the first reservoir and the second reservoir. The first blocking body closes a top edge of a reservoir body of the first reservoir. The movable member seals a bottom edge of a reservoir body the second reservoir. A total volume compartment is defined by the first blocking body, the first reservoir, the neck part, the second reservoir and the movable member collaboratively. When the axial pushing force is applied to the movable member, the movable member is linearly moved toward the neck part in a longitudinal

$357970.1 (GHMatters) P99405.AU NIOUSHAA 11 2013312546 09 Apr 2015 direction and the piston part, so that a volume distribution status of the volume compartment is correspondingly changed.

[0066] Preferably, the movable member includes a piston part, and the piston part is accommodated within the second reservoir and movable within the second reservoir.

[0067] Preferably, the movable member further includes a push rod, and the push rod is connected with the piston part, wherein when an axial pushing force is applied to the push rod, the piston part is pushed by the push rod in a longitudinal direction and the piston part is linearly moved toward the neck part, so that the total volume compartment is decreased; or when an axial pulling force is applied to the push rod, the piston part is pulled by the push rod in a longitudinal direction and the piston part is linearly moved away from the neck part, so that the total volume compartment is increased.

[0068] Preferably, an end of the push rod is connected with the piston part and integrally formed with the piston part; or an end of the push rod is hooked on the piston part.

[0069] Preferably, the movable member further includes a bottom cover, and the bottom cover is movably disposed on the bottom edge of the reservoir body the second reservoir, wherein when the bottom cover is moved toward the neck part, the piston part is correspondingly pushed by the bottom cover and the piston part is moved toward the neck part, so that the total volume compartment is decreased; or when the bottom cover is moved away from the neck part, the piston part is correspondingly pulled by the bottom cover and the piston part is moved away from the neck part, so that the total volume compartment is increased.

[0070] From the above descriptions, the present invention provides the centrifugal tube structure. The centrifugal tube structure has the neck part with a narrower cross section area. Consequently, the operator can extract the fractionated solution part more easily. In accordance with the design of the centrifugal tube structure, the first blocking body is fixed on the first reservoir. Since the first blocking body and first reservoir cannot be separated from each other, the centrifugal tube structure of the present invention can ensure the single-use purpose. Moreover, because of the simplified structure, the centrifugal tube structure is easily fabricated and facilitates mass production. In accordance with the design of the centrifugal tube structure, the bottom cover is fixed on the bottom edge of a reservoir body of the second reservoir, so that the position of the

$357970.1 (GHMallers) P99405.AU NIOUSHAA 12 2013312546 09 Apr 2015 piston part is limited by the bottom cover. Moreover, the movable member is designed to receive an axial force so as to push the piston part. While the operator extracts the fractionated solution part, the components of the external force in other directions are not applied to the centrifugal tube structure. Consequently, the possibility of swinging the centrifugal tube structure will be minimized.

[0071] The above objects and advantages of the present invention will become more readily apparent to those ordinarily skilled in the art after reviewing the following detailed description and accompanying drawings, in which:

BRIEF DESCRIPTION OF THE DRAWINGS

[0072] FIG. 1 is a schematic cross-sectional view illustrating a conventional bio device; [0073] FIG. 2 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a first embodiment of the present invention before the blood is pushed up; [0074] FIG. 3 is a schematic cross-sectional view illustrating the centrifugal tube structure according to the first embodiment of the present invention after the blood is pushed up and a syringe is inserted; [0075] FIG. 4 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a second embodiment of the present invention; [0076] FIG. 5 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a third embodiment of the present invention; [0077] FIG. 6 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a fourth embodiment of the present invention; [0078] FIG. 7 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a fifth embodiment of the present invention; [0079] FIG. 8 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a sixth embodiment of the present invention; [0080] FIG. 9 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a seventh embodiment of the present invention; [0081] FIG. 10 is a schematic cross-sectional view illustrating a centrifugal tube structure according to an eighth embodiment of the present invention; and

6357970_1 (GHMatters) P99405.AU NIOUSHAA 13 2013312546 09 Apr 2015 [0082] FIG. 11 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a ninth embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

[0083] The present invention will now be described more specifically with reference to the following embodiments. It is to be noted that the following descriptions of preferred embodiments of this invention are presented herein for purpose of illustration and description only. In the following embodiments and drawings, the elements irrelevant to the concepts of the present invention are omitted and not shown.

[0084] The spirit of the present invention is to provide a centrifugal tube structure. The centrifugal tube structure is used for containing the blood which is intended to be separated. Moreover, the blood is blended with a small amount of anticoagulant. The anticoagulant can prevent the coagulation of the blood during the operation. After the blood is sealed in the centrifugal tube structure, the centrifugal tube structure containing the blood is placed in a general centrifuge device and then the centrifuge device starts. The principles of blood fractionation will be illustrated as follows. Generally, the ingredients of the blood have different densities. After the centrifugal tube structure containing the blood is centrifuged by the centrifuge device, the blood stands for a certain time period. Consequently, the blood in the centrifugal tube structure is clearly fractionated into three layers of solution parts, including an upper solution part, a middle solution part and a lower solution part. The upper solution part is a plasma layer. The middle solution part is a buffy coat layer. The lower solution part is an erythrocyte layer. Since the buffy coat layer between the plasma layer and the erythrocyte layer is rich in growth factors, the buffy coat layer is helpful for repairing the aging tissue, promoting metabolism and promoting the newborn skin. Consequently, the buffy coat layer is often applied to the treatment process in cosmetic medicine, and its commercial value is very high. However, since the buffy coat layer is very thin, it is difficult for the operator to extract the buffy coat layer. The centrifugal tube structure is specially designed for facilitating the operator to extract the thin layer solution. In an embodiment, the thin layer solution is the buffy coat layer.

[0085] Please refer to FIGS. 2~4. FIGS. 2 and 3 are schematic cross-sectional views illustrating a centrifugal tube structure according to a first embodiment of the present invention before and after the blood is pushed up. FIG. 4 is a schematic

$357970.1 (GHMatters) P99405.AU NIOUSHAA 14 2013312546 09 Apr 2015 cross-sectional view illustrating a centrifugal tube structure according to a second embodiment of the present invention. The centrifugal tube structure comprises a tube body 2. The tube body 2 comprises a first reservoir 20, a second reservoir 22 and a neck part 21. The neck part 21 is in connected with the first reservoir 20 and the second reservoir 22. The cross section area of the neck part 21 is smaller than the cross section area of the first reservoir 20 and the cross section area of the second reservoir 22. That is, the neck part 21 of the centrifugal tube structure has a narrow path. Consequently, the outer appearance of the centrifugal tube structure is hourglass-shaped.

[0086] Moreover, the centrifugal tube structure further comprises a first blocking body 3 and a second blocking body 4. The first blocking body 3 is fixed and positioned on a top edge of a reservoir body of the first reservoir 20, so that the first blocking body 3 is immobile. The second blocking body 4 is movably coupled to a bottom edge of a reservoir body of the second blocking body 4. Due to this arrangement, a total volume compartment 5 is defined by the first blocking body 3, the first reservoir 20, the neck part 21, the second reservoir 22 and the second blocking body 4 collaboratively. Moreover, by moving the second blocking body 4, the volume distribution status of the total volume compartment 5 is correspondingly changed. On the other hand, since the first blocking body 3 is fixed on the first reservoir 20, a fixed volume compartment 200 is defined by the first blocking body 3 and the first reservoir 20 collaboratively. Similarly, since the second blocking body 4 is movably disposed within the second reservoir 22, a variable volume compartment 220 is defined by the second blocking body 4 and the second reservoir 22 collaboratively. Moreover, a communication channel 210 is defined by the neck part 21. The communication channel 210 is in communication with the fixed volume compartment 200 and the variable volume compartment 220. In other words, the total volume compartment 5 is composed of the fixed volume compartment 200, the communication channel 210 and the variable volume compartment 220.

[0087] It is noted that the way of fixing the first blocking body 3 on the first reservoir 20 is not restricted. In an embodiment, the first blocking body 3 and the first reservoir 20 are integrally formed with each other in the fabricating process (see FIGS. 2 and 3). Alternatively, as shown in FIG. 4, the first reservoir 20 further comprises a fixing means 205 and the first blocking body 3 is a fixing cover 35. The fixing cover 35 is engaged with the fixing means 205 of the first reservoir 20. Due to the fixing means 205,

6357970_1 (GHMatters) P99405.AU NIOUSHAA 15 2013312546 09 Apr 2015 the fixing cover 35 is fixed on the first reservoir 20. The first blocking body 3 may be fixed on a first opening part 201 of the first reservoir 20 by a locking means, an embedding means, an adhering means or any other appropriate fixing means. Due to this arrangement, the first blocking body 3 cannot be easily removed from the first reservoir 20. Consequently, the possibility of allowing the unscrupulous vendor to wash the centrifugal tube structure and repeatedly use the centrifugal tube structure will be minimized. Since blood may carry infectious bacteria and viruses, the centrifugal tube structure must be single-use disposable.

[0088] Please refer to FIGS. 2 and 3. When the centrifugal tube structure is in the usage state, the centrifugal tube structure is in vertical arrangement. The first blocking body 3 is located at the upper side, and the second blocking body 4 is located at the lower side. The blood is contained in the total volume compartment 5. Consequently, after the blood fractionation is completed, the blood in the total volume compartment 5 is fractionated into three layers of solution parts. If the second blocking body 4 is pushed up toward the neck part 21, the total volume compartment 5 is decreased and the three layers of solution parts are ascended. Whereas, if the second blocking body 4 is pulled away from the neck part 21, the total volume compartment 5 is increased and the three layers of solution parts are descended. That is, the altitudes of the three layers of solution parts may be adjusted by operating the second blocking body 4. Since the three layers of solution parts have their fixed volumes and the cross section area of the neck part 21 is reduced, the layer of solution part which is adjusted to the neck part 21 has increased thickness. Under this circumstance, the easy of extracting this layer of solution part by the operator is largely increased.

[0089] Please refer to FIGS. 2 and 3 again. The first blocking body 3 has a vent 202 and a liquid inlet 203. While the centrifuging action is performed, the blood within the centrifugal tube structure may leak out of the vent 202 or liquid inlet 203. For preventing the blood from leaking out of the vent 202 or liquid inlet 203, the first blocking body 3 further comprises two plugs 204 and 204’. While the centrifuging action is performed, the liquid inlet 203 and the vent 202 are sealed by the two plugs 204 and 204’ of the first blocking body 3. The plug 204’ has a perforation 204’a. A syringe needle 6 may be penetrated through the perforation 204’a of the plug 204’. The vent 202 is aligned with the neck part 21. When the syringe needle 6 is penetrated through the

$357970_1 (GHMallers) P99405.AU NIOUSHAA 16 2013312546 09 Apr 2015 perforation 204’a, a portion of the syringe needle 6 is introduced into the first reservoir to extract the solution parts within the total volume compartment 5. Of course, a desired layer of solution part may be extracted according to the practical requirements of the user.

[0090] In particular, the liquid inlet 203 is located beside the vent 202. An external liquid may be introduced into the total volume compartment 5 through the liquid inlet 203. The total volume compartment 5 is in communication with the surroundings through the vent 202. While the blood is introduced into the total volume compartment 5, a portion of the total volume compartment 5 is occupied by the liquid. Due to the arrangement of the vent 202, a portion of the gas originally contained in the total volume compartment 5 is ejected to the surroundings through the vent 202.

[0091] Please refer to FIGS. 2-4. The second blocking body 4 comprises a piston part 41. The piston part 41 is accommodated within the second reservoir 22 and movable within the second reservoir 22. By moving the piston part 41, the volume distribution status of the total volume compartment 5 is correspondingly changed. For example, the operator may push the piston part 41 to decrease the total volume compartment 5 or pull the piston part 41 to increase the total volume compartment 5. Moreover, if an external force is applied to the piston part 41 to result in deformation of the piston part 41, the total volume compartment 5 may be selectively decreased or increased. In other words, various modifications of the piston part 41 of the centrifugal tube structure may be made while retaining the teachings of the invention.

[0092] Please refer to FIGS. 5 and 6. FIG. 5 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a third embodiment of the present invention. FIG. 6 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a fourth embodiment of the present invention. It is noted that the way of fixing the first blocking body 3 on the first reservoir 20 is not restricted. As shown in FIG. 5, the first blocking body 3 and the first reservoir 20 are integrally formed with each other. As shown in FIG. 5, the first blocking body 3 is a fixing cover that is engaged with the fixing means of the first reservoir 20. The relationship between the first blocking body 3 and the first reservoir 20 is presented herein for purpose of illustration and description only. Consequently, the first blocking body 3 may be fixed on the first reservoir 20 by any appropriate fixing means.

6357970_1 (GHMatters) P99405.AU NIOUSHAA 17 2013312546 09 Apr 2015 [0093] In the embodiment of FIG. 5, a push rod 42 is used to push the piston part 41. In particular, the second blocking body 4 further comprises the push rod 42. The push rod 42 is connected with the piston part 41. An axial direction of the push rod 42 is in parallel with a longitudinal direction of the tube body 2. Preferably, the axial direction of the push rod 42 is identical to the longitudinal direction of the tube body 2. Consequently, when an axial pushing force is applied to the push rod 42, the piston part 41 is pushed by the push rod 42 in the longitudinal direction. Under this circumstance, since the piston part 41 is linearly moved toward the neck part 21, the total volume compartment 5 is decreased. Similarly, when an axial pulling force is applied to the push rod 42, the piston part 41 is pulled by the push rod 42 in the longitudinal direction. Under this circumstance, since the piston part 41 is linearly moved away from the neck part 21, the total volume compartment 5 is increased. It is noted that the way of connecting the push rod 42 and the piston part 41 is not restricted. For example, an end of the push rod 42 and the piston part 41 are integrally formed with each other. Alternatively, an end of the push rod 42 is hooked on the piston part 41. Consequently, the operator may operate the push rod 42 to control the upward or downward movement of the piston part 41. These examples are presented herein for purpose of illustration and description only.

[0094] Moreover, by moving the piston part 41, the volume distribution status of the total volume compartment 5 is correspondingly changed. For example, the operator may push the piston part 41 to decrease the total volume compartment 5 or pull the piston part 41 to increase the total volume compartment 5. Moreover, if an external force is applied to the piston part 41 to result in deformation of the piston part 41, the total volume compartment 5 may be selectively decreased or increased. In other words, various modifications of the piston part 41 of the centrifugal tube structure may be made while retaining the teachings of the invention.

[0095] As shown in FIG. 6, the push rod 42 is used to push the piston part 41. In addition, the centrifugal tube structure of this embodiment further comprises a bottom cover 43. In particular, the second blocking body 4 further comprises the bottom cover 43. The bottom cover 43 is fixed on a bottom edge of the reservoir body of the second bottom cover 43. Moreover, by the bottom cover 43, the position of the piston part 41 is limited between the bottom cover 43 and the neck part 21. In this embodiment, a hollow part 430 is formed in a center of the bottom cover 43. The push rod 42 is penetrated

6357970.1 (GHMallers) P99405.AU NIOUSHAA 18 2013312546 09 Apr 2015 through the hollow part 430 and connected with the piston part 41. When an external force is applied to the push rod 42, the piston part 41 is pushed toward the neck part 21 by the push rod 42 or the piston part 41 is pulled away from the neck part 21 by the push rod 42. Consequently, the fractionated solution parts of the blood within the centrifugal tube structure are ascended or descended. The actions are similar to those of the previous embodiment. Moreover, due to the arrangement of the bottom cover 43, the piston part 41 is not over-pulled to be escaped from the second reservoir 22.

[0096] Please refer to FIGS. 7 and 8. FIG. 7 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a fifth embodiment of the present invention. FIG. 8 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a sixth embodiment of the present invention. In these two embodiments, the piston part 41 is pushed by a bottom cover. In particular, the second blocking body 4 further comprises a bottom cover 44 or 45. The bottom cover 44 or 45 is movably fixed on the bottom edge of the reservoir body of the second reservoir 22. Consequently, the piston part 41 is arranged between the bottom cover 44 or 45 and the neck part 21. The bottom cover 44 or 45 is connected with the piston part 41. The way of connecting the bottom cover 44 or 45 with the piston part 41 is not restricted. For example, the bottom cover 44 or 45 and the piston part 41 may be integrally formed with each other, or engaged with each other, or abutted against each other. When the bottom cover 44 or 45 is moved toward the neck part 21, the piston part 41 is pushed by the bottom cover 44 or 45. Consequently, the piston part 41 is correspondingly moved toward the neck part 21 to decrease the total volume compartment 5. Similarly, when the bottom cover 44 or 45 is moved away from the neck part 21, the piston part 41 is pulled by the bottom cover 44 or 45. Consequently, the piston part 41 is correspondingly moved away from the neck part 21 to increase the total volume compartment 5.

[0097] Please refer to FIGS. 7 and 8 again. As shown in FIG. 7, the movement of the bottom cover 44 relative to the second reservoir 22 is achieved by the following mechanism. A surface of the bottom cover 44 has male threads 221, and a surface of the second reservoir 22 has female threads 222. The male threads 221 match the female threads 222. When an external force in a tangential direction is applied to bottom cover 44, the bottom cover 44 is rotated about the axial direction and moved in the axial direction.

6357970_1 (GHMatters) P99405.AU NIOUSHAA 19 2013312546 09 Apr 2015

It is noted that the movement of the bottom cover relative to the second reservoir 22 is not restricted.

[0098] As shown in FIG. 8, the bottom cover 45 movably coupled to a bottom edge of a reservoir body of the second blocking body 4. When an axial pushing force is applied to the bottom cover 45, the bottom cover 45 is moved toward the neck part 21 in the longitudinal direction. Moreover, since the piston part 41 is pushed by the bottom cover 45, the piston part 41 is moved toward the neck part 21 and the total volume compartment 5 is decreased. Alternatively, when an axial pulling force is applied to the bottom cover 45, the bottom cover 45 is moved away from the neck part 21 in the longitudinal direction. Since the piston part 41 is pulled by the bottom cover 45, the piston part 41 is moved away from the neck part 21 and the total volume compartment 5 is increased. Especially, the bottom cover 45 has a convex structure 224, and the second reservoir 22 has a convex structure 223. The concave structure 223 match the convex structure 224, so that the bottom cover 45 is slidable relative to the second reservoir 22.

[0099] In the above embodiments, the female threads 222 of the second reservoir 22 and the convex structure 223 of the second reservoir 22 are possible moving means of the second reservoir 22. It is noted that the examples of the moving means are not restricted.

[00100] FIG. 9 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a seventh embodiment of the present invention. In this embodiment, the first blocking body 3 of the centrifugal tube structure is a fixing cover 35. The fixing cover 35 is engaged with a fixing means 205 of the first reservoir 20. Due to the fixing means 205, the fixing cover 35 is fixed on the first reservoir 20. Moreover, the second blocking body 4 further comprises the push rod 42. The push rod 42 is connected with the piston part 41. An axial direction of the push rod 42 is in parallel with the longitudinal direction of the centrifugal tube structure. Consequently, the push rod 42 can be pushed by the user.

[00101] FIG. 10 is a schematic cross-sectional view illustrating a centrifugal tube structure according to an eighth embodiment of the present invention. In this embodiment, the first blocking body 3 of the centrifugal tube structure is a fixing cover 35. The fixing cover 35 is engaged with a fixing means 205 of the first reservoir 20. Due to the fixing means 205, the fixing cover 35 is fixed on the first reservoir 20. Moreover, the

$357970.1 (GHMatters) P99405.AU NIOUSHAA 20 2013312546 09 Apr 2015 second blocking body 4 further comprises a bottom cover 46. The bottom cover 46 is a movable bottom cover. When an external force in a tangential direction is applied to bottom cover 46, the bottom cover 46 is rotated about the axial direction and moved in the axial direction, so that the piston part 41 is moved toward the neck part 21 by the bottom cover 44. And/or, the bottom cover 46 may be served as a stopper for limiting the piston part 41 from being over-pulled out of the second reservoir 22. Moreover, the second blocking body 4 further comprises a push rod 42. The push rod 42 is connected with the piston part 41. An axial direction of the push rod 42 is in parallel with the longitudinal direction of the centrifugal tube structure. Consequently, when the push rod 42 is pushed by the user, the piston part 41 is correspondingly moved in the axial direction.

[00102] FIG. 11 is a schematic cross-sectional view illustrating a centrifugal tube structure according to a ninth embodiment of the present invention. In this embodiment, the first blocking body 3 of the centrifugal tube structure is a fixing cover 35. The fixing cover 35 is engaged with a fixing means 205 of the first reservoir 20. Due to the fixing means 205, the fixing cover 35 is fixed on the first reservoir 20. Moreover, the second blocking body 4 further comprises a bottom cover 44. When an external force in a tangential direction is applied to bottom cover 44, the bottom cover 44 is rotated about the axial direction and moved in the axial direction. Consequently, the piston part 41 is pushed toward the neck part 21 by the bottom cover 44.

[00103] From the above descriptions, the present invention provides the centrifugal tube structure. The centrifugal tube structure has the neck part with a narrower cross section area. Consequently, the operator can extract the fractionated solution part more easily. In accordance with the design of the centrifugal tube structure, the first blocking body is fixed on the first reservoir. Since the first blocking body and first reservoir cannot be separated from each other, the centrifugal tube structure of the present invention can ensure the single-use purpose. Moreover, because of the simplified structure, the centrifugal tube structure is easily fabricated and facilitates mass production. In accordance with the design of the centrifugal tube structure, the bottom cover is fixed on the bottom edge of a reservoir body of the second reservoir, so that the position of the piston part is limited by the bottom cover. Moreover, a movable member including the piston part and the push rod is designed to receive an axial force so as to push the piston part. While the operator extracts the fractionated solution part, the components of the

6357970_1 (GHMatters) P99405.AU NIOUSHAA 21 2013312546 09 Apr 2015 external force in other directions are not applied to the centrifugal tube structure. Consequently, the possibility of swinging the centrifugal tube structure will be minimized.

[00104] While the invention has been described in terms of what is presently considered to be the most practical and preferred embodiments, it is to be understood that the invention needs not be limited to the disclosed embodiment. On the contrary, it is intended to cover various modifications and similar arrangements included within the spirit and scope of the appended claims which are to be accorded with the broadest interpretation so as to encompass all such modifications and similar structures.

[00105] In the claims which follow and in the preceding description of the invention, except where the context requires otherwise due to express language or necessary implication, the word “comprise” or variations such as “comprises” or “comprising” is used in an inclusive sense, i.e. to specify the presence of the stated features but not to preclude the presence or addition of further features in various embodiments of the invention.

[00106] It is to be understood that, if any prior art publication is referred to herein, such reference does not constitute an admission that the publication forms a part of the common general knowledge in the art, in Australia or any other country.

6357970.1 (GHMallers) P99405.AU NIOUSHAA

Claims

THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:

1. A centrifugal tube structure, comprising: a tube body comprising a first reservoir, a second reservoir and a neck part connected with the first reservoir and the second reservoir; a first blocking body fixed on the first reservoir, wherein a fixed volume compartment is defined by the first blocking body and the first reservoir collaboratively; and a second blocking body comprising a bottom cover movably disposed at a bottom of the tube body and a piston part arranged between the neck part and the bottom cover and movable with a push-pull force toward or away from the neck part, wherein a variable volume compartment is defined by the piston part and the second reservoir collaboratively, and the bottom cover serves as a stopper for limiting the piston part from being over-pulled out of the second reservoir, wherein a communication channel is defined by the neck part, and the communication channel is in communication with the fixed volume compartment and the variable volume compartment.
2. The centrifugal tube structure according to claim 1, wherein the first reservoir has a first opening part and the first blocking body is fixed on the first opening part of the first reservoir; or the first blocking body and the first reservoir are integrally formed with each other.
3. The centrifugal tube structure according to claim 2, wherein the first blocking body has a vent and a liquid inlet, the vent is aligned with the neck part, the fixed volume compartment is in communication with surroundings through the vent, and the liquid inlet is located beside the vent, wherein the first blocking body further comprises two plugs, the liquid inlet and the vent are sealed by the two plugs, respectively, wherein the plug that seals the vent has a perforation, and a syringe needle is permitted to be introduced into the first reservoir through the perforation of the plug.
4. The centrifugal tube structure according to any one of the preceding claims, wherein the second blocking body further comprises a push rod, a center of the bottom cover is a hollow part, and the push rod is penetrated through the hollow part and connected with the piston part, wherein the piston part is pushed toward the neck part or pulled away from the neck part by the push rod when an external force is applied to the push rod for providing the push-pull force.
5. The centrifugal tube structure according to any one of the preceding claims, wherein a surface of the bottom cover has male threads, a surface of the second reservoir has female threads, the male threads match the female threads, and the bottom cover is moved in an axial direction when the bottom cover is rotated about the axial direction; or the bottom cover has a convex structure, the second reservoir has a concave structure, the convex structure matches the concave structure, so that the bottom cover is slidable relative to the second reservoir.
6. The centrifugal tube structure according to any one of the preceding claims, wherein the second reservoir includes a concave structure, the bottom cover has a convex structure, and the convex structure matches the concave structure, so that the bottom cover is slidable relative to the second reservoir for providing the push-pull force, so as to drive the piston part to move toward or away from the neck part.