CN220246110U - Multi-bin cell inoculation culture device - Google Patents
Multi-bin cell inoculation culture device Download PDFInfo
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- CN220246110U CN220246110U CN202321488693.4U CN202321488693U CN220246110U CN 220246110 U CN220246110 U CN 220246110U CN 202321488693 U CN202321488693 U CN 202321488693U CN 220246110 U CN220246110 U CN 220246110U
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- inoculation culture
- cell inoculation
- rack
- cell
- detachable
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- 238000011081 inoculation Methods 0.000 title claims abstract description 82
- 239000000725 suspension Substances 0.000 claims abstract description 11
- 229910001220 stainless steel Inorganic materials 0.000 claims description 12
- 239000010935 stainless steel Substances 0.000 claims description 12
- 230000002572 peristaltic effect Effects 0.000 claims description 10
- 238000009434 installation Methods 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 7
- 238000003780 insertion Methods 0.000 claims description 6
- 230000037431 insertion Effects 0.000 claims description 6
- 238000005192 partition Methods 0.000 claims description 6
- 238000003466 welding Methods 0.000 claims description 4
- 238000010899 nucleation Methods 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 15
- 210000004027 cell Anatomy 0.000 description 35
- 238000004113 cell culture Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005429 filling process Methods 0.000 description 1
- 210000000497 foam cell Anatomy 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a multi-bin cell inoculation culture device, which relates to the technical field of inoculation culture devices and aims to solve the technical problem that the inoculation culture device in the prior art cannot perform stable inoculation culture; the inside detachable mount subassembly that is provided with of cell inoculation culture rack, inside fixed unremovable support frame that is provided with of cell inoculation culture rack, inside suspension type inoculation culture subassembly that is provided with of cell inoculation culture rack, cell inoculation culture rack outside is provided with control drive assembly, inside jacket mechanism that is provided with of detachable mount subassembly, this inoculation culture apparatus can play the effect of quick assembly disassembly change through detachable mount subassembly, utilizes suspension type inoculation culture subassembly can play stable protection inoculation culture's effect.
Description
Technical Field
The utility model belongs to the technical field of inoculation culture devices, and particularly relates to a multi-bin cell inoculation culture device.
Background
The cell culture process flow is shake flask cell expansion culture-Rocker cell expansion-SUB cell culture, wherein the Rocker cell expansion and SUB cell culture adopts a throwing process, namely, a disposable bag is used for containing cell liquid and relevant culture medium and replenishing solution, the disposable bag needs to be manually transferred to a target position when the process is inoculated and filled with solution, the disposable reaction bag is easy to damage in the transfer process, the risk of pollution of the cell liquid and the solution is caused, the cell culture needs to be carried out under aseptic conditions, the aseptic welder connecting pipeline and peristaltic pump are used in the inoculation and solution filling processes, so that aseptic conditions and stable transportation rate are ensured, operations such as welding-pumping-welding and the like are repeated continuously, operators repeatedly replace and carry equipment, and when the cell liquid is pumped by the peristaltic pump, good angle support is required to be manually given, bubbles generated by improper operations are easy to damage cells, foam accumulation is easy to influence the environment in the cell culture process, the conventional method and device are easy to meet the requirements, and simultaneously, the artificial transfer efficiency is improved by designing the same pump and carrying process, the manual operation is improved, and the manual operation is carried out by the pump is carried out under the conditions.
Therefore, in order to solve the problem that the inoculation culture device cannot perform multi-bin stable inoculation culture, a solution is needed to improve the use situation of the inoculation culture device.
Disclosure of Invention
(1) Technical problem to be solved
Aiming at the defects of the prior art, the utility model aims to provide a multi-bin cell inoculation culture device which aims at solving the technical problem that the multi-bin stable inoculation culture cannot be realized by the inoculation culture device in the prior art.
(2) Technical proposal
In order to solve the technical problems, the utility model provides a multi-bin cell inoculation culture device, which comprises a cell inoculation culture rack, a side support column body arranged on the inside of the cell inoculation culture rack, and a top end hanging component connected with the side support column body; the cell inoculation culture rack is characterized in that a detachable fixing rack assembly is arranged inside the cell inoculation culture rack, a fixed non-detachable support frame is arranged inside the cell inoculation culture rack, a suspension type inoculation culture assembly is arranged inside the cell inoculation culture rack, a control driving assembly is arranged outside the cell inoculation culture rack, a jacket mechanism is arranged inside the detachable fixing rack assembly, and a peristaltic pump is arranged inside the control driving assembly.
When the inoculation culture device of the technical scheme is used, the effect of quick assembly disassembly and replacement can be achieved through the arrangement of the detachable fixing frame assembly, so that the use functionality of the cell inoculation culture frame is improved, the effect of multi-bin stable protection inoculation culture can be achieved through the arrangement of the suspension type inoculation culture assembly, and the cell inoculation culture efficiency is further improved.
Further, the top hanging component comprises a disposable bag hanging hook, the detachable fixing frame component comprises a detachable bracket rod, and the disposable bag hanging hook can play a role in stably hanging and placing the top.
Further, the dismouting cradling piece outside fixedly connected with inserts installation branch, insert the inside internally mounted hole of having seted up of installation branch, the inside patchhole of having seted up of side support cylinder can play the effect of location insertion installation through the setting of inserting installation branch and patchhole.
Further, the outside mounting hole has been seted up to the side support cylinder is inside, the inside mounting hole is provided with the mounting with the outside mounting hole outside, through the setting of outside mounting hole and mounting, can play quick assembly disassembly's effect.
Further, the detachable fixing frame assembly comprises a disposable bag supporting plate, a detachable disposable biological reaction bag is arranged on the outer side of the disposable bag supporting plate, a pipeline passage is formed in the disposable bag supporting plate, an undetachable rotating bearing is arranged on the outer side of the disposable bag supporting plate, and the effect of matching rotation installation can be achieved through the arrangement of the disposable bag supporting plate and the undetachable rotating bearing.
Further, the inside stainless steel that is included of jacket mechanism goes up to press from both sides, the stainless steel goes up the clamp outside and is provided with disposable bag and places the department, but stainless steel goes up the clamp outside and is provided with rotatory fixing bolt, but through the stainless steel go up clamp and the setting of rotatory fixing bolt, makes disposable bag can stably place the use.
Further, the suspension type inoculation culturing component is internally provided with a cell inoculation culturing box, a partition board in a sub-bin is arranged in the cell inoculation culturing box, the control driving component is internally provided with a sterile welding machine connected with a peristaltic pump, and the effect of multi-bin stable protection inoculation culturing can be achieved through the arrangement of the cell inoculation culturing box and the partition board in the sub-bin.
(3) Advantageous effects
Compared with the prior art, the utility model has the following beneficial effects:
1. the utility model can play the role of quick disassembly and replacement by arranging the detachable fixing frame component, thereby improving the use functionality of the cell inoculation culture frame.
2. According to the utility model, through the arrangement of the suspension type inoculation culture assembly, the effect of multi-bin stable protection inoculation culture can be achieved, and the cell inoculation culture efficiency is further improved.
Drawings
FIG. 1 is a schematic diagram showing the structure of an embodiment of an inoculating culture apparatus of the present utility model;
FIG. 2 is a schematic view showing the structure of a disposable bioreactor bag according to an embodiment of the inoculation culture apparatus of the present utility model;
FIG. 3 is a schematic view showing the structure of a jacket mechanism of an embodiment of the inoculating culture apparatus of the present utility model;
FIG. 4 is a schematic view showing the structure of an insertion mounting strut of an embodiment of an inoculating culture device of the present utility model;
FIG. 5 is a schematic diagram showing the structure of a cell inoculation incubator in one embodiment of the inoculation culture apparatus of the present utility model.
The marks in the drawings are: 1. a cell inoculation culture rack; 2. a side support column; 3. a top end hanging component; 4. a detachable fixing frame component; 5. fixing the non-detachable support frame; 6. a suspension inoculation and culture assembly; 7. controlling the driving assembly; 8. a jacket mechanism; 9. a peristaltic pump; 10. a disposable bag hanging hook; 11. disassembling and assembling the bracket rod; 12. inserting a mounting support rod; 13. an internal mounting hole; 14. an insertion hole; 15. an outer mounting hole; 16. a fixing member; 17. a disposable bag support plate; 18. a conduit path; 19. a non-detachable swivel bearing; 20. stainless steel upper clamp; 21. the disposable bag is placed; 22. a rotatable fixing bolt; 23. a cell inoculation incubator; 24. dividing the cabin into inner partition boards; 25. a sterile welder; 26. a disposable biological reaction bag.
Detailed Description
As shown in fig. 1 to 4, the present utility model provides a multi-chamber cell inoculation culture apparatus, comprising a cell inoculation culture rack 1, a side support column 2 arranged on the inside of the cell inoculation culture rack 1, and a top hanging component 3 connected to the side support column 2; the cell inoculation cultivates frame 1 inside is provided with removable mount subassembly 4, cell inoculation cultivates frame 1 inside is provided with fixed non-removable support frame 5, cell inoculation cultivates frame 1 inside is provided with suspension type inoculation cultivates subassembly 6, cell inoculation cultivates frame 1 outside is provided with control drive assembly 7, removable mount subassembly 4 inside is provided with jacket mechanism 8, control drive assembly 7 inside is including peristaltic pump 9.
For this embodiment, the installation position of the peristaltic pump 9 can be adjusted and set according to the design requirement.
The top hanging component 3 comprises a disposable bag hanging hook 10, the detachable fixing frame component 4 comprises a detachable support rod 11, an inserting and mounting support rod 12 is fixedly connected to the outer side of the detachable support rod 11, an inner mounting hole 13 is formed in the inserting and mounting support rod 12, an inserting hole 14 is formed in the side supporting column body 2, an outer side mounting hole 15 is formed in the side supporting column body 2, a fixing piece 16 is arranged outside the inner mounting hole 13 and the outer side mounting hole 15, and the fixing piece 16 can be set to be a standard piece such as a screw, a bolt and the like.
In addition, the inside disposable bag backup pad 17 that is provided with of removable mount subassembly 4, disposable bag backup pad 17 outside is provided with removable disposable biological reaction bag 26, the inside pipeline passageway 18 of seting up of disposable bag backup pad 17, disposable bag backup pad 17 outside is provided with non-removable swivel bearing 19, press from both sides on the cover mechanism 8 inside including stainless steel 20, the stainless steel 20 outside is provided with disposable bag and places department 21, the stainless steel 20 outside is provided with rotatable fixing bolt 22, the inside cell inoculation incubator 23 that is provided with of suspension type inoculation cultivation subassembly 6, the inside baffle 24 in the branch storehouse that is provided with of cell inoculation incubator 23, the inside aseptic welding machine 25 that is connected with peristaltic pump 9 that is included of control drive subassembly 7.
It should be noted here that the distribution intervals and the number of the partition plates 24 in the sub-compartments can be adjusted accordingly according to actual needs.
When the inoculation culture device of the technical scheme is used, firstly, the effect of stably hanging and placing the top can be achieved through the disposable bag hanging hook 10, the effect of positioning, inserting and installing can be achieved through the inserting and installing support rod 12 and the inserting hole 14, then the effect of quick dismounting can be achieved through the outer side installing hole 15 and the fixing piece 16, the effect of matching and rotating and installing can be achieved through the disposable bag supporting plate 17 and the non-detachable rotating bearing 19, then the disposable bag can be stably placed and used through the stainless steel upper clamp 20 and the rotatable fixing bolt 22, and finally the effect of multi-bin stable protection inoculation culture can be achieved through the cell inoculation incubator 23 and the partition plate 24 in the sub bin.
Claims (7)
1. The multi-bin cell inoculation culture device comprises a cell inoculation culture rack, a side support column body arranged on the inside of the cell inoculation culture rack, and a top end hanging component connected with the side support column body; the cell inoculation culture rack is characterized in that a detachable fixing rack assembly is arranged inside the cell inoculation culture rack, a fixed non-detachable support frame is arranged inside the cell inoculation culture rack, a suspension type inoculation culture assembly is arranged inside the cell inoculation culture rack, a control driving assembly is arranged outside the cell inoculation culture rack, a jacket mechanism is arranged inside the detachable fixing rack assembly, and a peristaltic pump is arranged inside the control driving assembly.
2. The multi-chamber cell seeding and culturing device according to claim 1, wherein the top hanging component comprises a disposable bag hanging hook, and the detachable fixing frame component comprises a detachable bracket rod.
3. The multi-bin cell inoculation culture device according to claim 2, wherein the outer side of the dismounting bracket rod is fixedly connected with an insertion installation supporting rod, an inner installation hole is formed in the insertion installation supporting rod, and an insertion hole is formed in the side support column.
4. A multi-chamber cell seeding and culturing device according to claim 3, wherein the side support column is internally provided with an outer mounting hole, and the inner mounting hole and the outer mounting hole are externally provided with a fixing member.
5. The multi-bin cell inoculation culture device according to claim 4, wherein the detachable fixing frame assembly comprises a disposable bag supporting plate, a detachable disposable biological reaction bag is arranged on the outer side of the disposable bag supporting plate, a pipeline passage is formed in the disposable bag supporting plate, and a non-detachable rotating bearing is arranged on the outer side of the disposable bag supporting plate.
6. The multi-bin cell inoculation culture device according to claim 5, wherein the jacket mechanism comprises a stainless steel upper clamp, a disposable bag placement part is arranged on the outer side of the stainless steel upper clamp, and a rotatable fixing bolt is arranged on the outer side of the stainless steel upper clamp.
7. The multi-bin cell inoculation and culture device according to claim 1, wherein the suspension type inoculation and culture assembly comprises a cell inoculation incubator, a partition plate in a bin is arranged in the cell inoculation incubator, and a sterile welding machine connected with a peristaltic pump is arranged in the control driving assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321488693.4U CN220246110U (en) | 2023-06-12 | 2023-06-12 | Multi-bin cell inoculation culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321488693.4U CN220246110U (en) | 2023-06-12 | 2023-06-12 | Multi-bin cell inoculation culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220246110U true CN220246110U (en) | 2023-12-26 |
Family
ID=89232980
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321488693.4U Active CN220246110U (en) | 2023-06-12 | 2023-06-12 | Multi-bin cell inoculation culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220246110U (en) |
-
2023
- 2023-06-12 CN CN202321488693.4U patent/CN220246110U/en active Active
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