CN220467977U - Cell culture is with fluid infusion device - Google Patents
Cell culture is with fluid infusion device Download PDFInfo
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- CN220467977U CN220467977U CN202321950134.0U CN202321950134U CN220467977U CN 220467977 U CN220467977 U CN 220467977U CN 202321950134 U CN202321950134 U CN 202321950134U CN 220467977 U CN220467977 U CN 220467977U
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- cell culture
- infusion
- liquid
- head
- way valve
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- 238000004113 cell culture Methods 0.000 title claims abstract description 65
- 238000001802 infusion Methods 0.000 title claims abstract description 35
- 239000012530 fluid Substances 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 59
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 13
- 230000001502 supplementing effect Effects 0.000 claims abstract description 12
- 230000007246 mechanism Effects 0.000 claims description 19
- 239000000725 suspension Substances 0.000 claims description 13
- 238000002637 fluid replacement therapy Methods 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 2
- 238000009630 liquid culture Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 10
- 230000008569 process Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 239000012930 cell culture fluid Substances 0.000 description 4
- 238000010367 cloning Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a liquid supplementing device for cell culture, which relates to the technical field of cell culture, and particularly relates to a liquid supplementing device for cell culture. This cell culture is with fluid infusion device carries out fluid infusion through using peristaltic pump drive conveyer pipe two inside cell culture liquid, and cell culture liquid is in the pipeline all the time and carries, avoids cell culture liquid to spill outside to reduce cell pollution's possibility, through setting up two infusion heads, utilizes electromagnetic three-way valve control conveyer pipe two and two independent intercommunication of infusion heads, can switch to another cell culture liquid in time after one bag cell culture liquid is used up, the condition that can not appear the interrupt.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a fluid infusion device for cell culture.
Background
Cell culture refers to a method for simulating in vitro in vivo environment to enable the living, growing, propagating and maintaining main structures and functions, the cell culture is also called a cell cloning technology, the normal noun in biology is a cell culture technology, the cell culture is an essential process for the whole bioengineering technology or one of the bioengineering technology, the cell culture is a large-scale cloning of cells, the cell culture technology can be a simple single cell or a little differentiated multicellular from one cell through a large number of culture, the cell culture technology is an essential link of the cloning technology, the cell culture technology is cloning of cells, and the cell culture technology is an important and common technology in a cell biology research method.
In the process of culturing cells in the incubator, the cells consume the culture solution in the incubator, so that staff is required to supplement the culture solution to the incubator regularly, and in the existing cell culturing process, a commonly used fluid supplementing mode is to use a syringe, inversely put the syringe in a bracket to connect a cell culturing bag, pour the culture solution into the inside, and the culture solution is easily poured out of the syringe in the process, and the open fluid supplementing mode increases the possibility of cell pollution. For this purpose we provide a fluid infusion device for cell culture.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model provides a liquid supplementing device for cell culture, which solves the problems in the prior art.
In order to achieve the above purpose, the utility model is realized by the following technical scheme: the utility model provides a cell culture is with fluid infusion device, includes workstation, infusion mechanism, suspension mechanism, infusion mechanism is including fixing the cylinder at the workstation top, the infusion head is installed at the top of cylinder, the bottom of infusion head is connected with conveyer pipe one, the bottom of conveyer pipe one is connected with electromagnetic three-way valve, electromagnetic three-way valve's bottom is connected with conveyer pipe two, the internally mounted of workstation has the peristaltic pump, suspension mechanism is including fixing the support frame at the workstation top.
Optionally, the number of the infusion heads is two, the infusion heads are symmetrically distributed about the electromagnetic three-way valve, and the two infusion heads are connected with the input port of the electromagnetic three-way valve through the first conveying pipe.
Optionally, the second conveying pipe passes through the inside of the pump head of the peristaltic pump, one end of the second conveying pipe, which is far away from the electromagnetic three-way valve, is connected with a liquid supplementing head, and the liquid supplementing head is arranged on the outer side of the workbench.
Optionally, the number of the suspension mechanisms is two, and the two suspension mechanisms are respectively positioned above the infusion head.
Optionally, the bottom of support frame is connected with the balladeur train, the bottom joint of balladeur train has the conveyer belt, the inboard of conveyer belt is connected with the drive rack, the bottom of conveyer belt is connected with the culture solution stores pylon.
Optionally, driving motor is installed at the top of support frame, driving motor's output shaft activity passes the support frame, driving motor output shaft's bottom is connected with the drive wheel, the drive wheel is in the same place with the drive rack meshing.
Optionally, the top of culture solution stores pylon is connected with the couple, the bottom of culture solution stores pylon is connected with the locating plate, the spacing groove has been seted up to the inside of locating plate.
The utility model provides a fluid infusion device for cell culture, which has the following beneficial effects:
1. this cell culture is with fluid infusion device carries out fluid infusion through using peristaltic pump drive conveyer pipe two inside cell culture liquid, and cell culture liquid is in the pipeline all the time and carries, avoids cell culture liquid to spill outside to reduce cell pollution's possibility, through setting up two infusion heads, utilizes electromagnetic three-way valve control conveyer pipe two and two independent intercommunication of infusion heads, can use two bags of cell culture liquid simultaneously, can switch to another bag of cell culture liquid in time after one bag of cell culture liquid is used up, can not appear the circumstances of interrupt.
2. According to the liquid supplementing device for cell culture, after one bag of cell culture liquid is used up, the driving wheel is driven to rotate by the driving motor to drive the conveying belt to move along the sliding frame, so that the conveying belt conveys the subsequent bagged cell culture liquid to the liquid conveying head, the liquid conveying head is pushed by the air cylinder to move upwards, and the liquid conveying head is inserted into the bagged cell culture liquid to automatically supplement the cell culture liquid.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic view of the infusion mechanism of the present utility model;
FIG. 3 is a schematic view of the structure of the inside of the workbench of the utility model;
FIG. 4 is a schematic view of a suspension mechanism according to the present utility model;
FIG. 5 is a schematic view of the structure of the support frame of the present utility model;
FIG. 6 is a schematic structural view of a culture solution hanger of the present utility model.
In the figure: 1. a work table; 2. a cylinder; 3. an infusion head; 4. a first conveying pipe; 5. an electromagnetic three-way valve; 6. a second conveying pipe; 7. a peristaltic pump; 8. a fluid supplementing head; 9. a support frame; 10. a carriage; 11. a conveyor belt; 12. a drive rack; 13. a driving motor; 14. a driving wheel; 15. a culture solution hanging frame; 16. a hook; 17. a positioning plate; 18. and a limit groove.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
Referring to fig. 1 to 3, the present utility model provides a technical solution: the liquid supplementing device for cell culture comprises a workbench 1, a liquid delivering mechanism and a suspension mechanism, wherein the liquid delivering mechanism comprises a cylinder 2 fixed at the top of the workbench 1, a liquid delivering head 3 is arranged at the top of the cylinder 2, the liquid delivering head 3 is pushed to move upwards by the cylinder 2 through the cylinder 2, the liquid delivering head 3 can be automatically inserted into cell culture liquid without manual operation, the bottom of the liquid delivering head 3 is connected with a first conveying pipe 4, the bottom of the first conveying pipe 4 is connected with an electromagnetic three-way valve 5, the number of the liquid delivering heads 3 is two and symmetrically distributed relative to the electromagnetic three-way valve 5, the two liquid delivering heads 3 are connected with the input port of the electromagnetic three-way valve 5 through the first conveying pipe 4, the two liquid delivering heads 3 are independently communicated with the two liquid delivering heads 3 by controlling the second conveying pipe 6 through the electromagnetic three-way valve 5, can use two bags of cell culture liquid simultaneously, can switch to another bag of cell culture liquid in time after one bag of cell culture liquid is used up, the condition that can not appear breaking off, the bottom of electromagnetism three-way valve 5 is connected with conveyer pipe two 6, the internally mounted of workstation 1 has peristaltic pump 7, peristaltic pump 7's model is L300-1FS, conveyer pipe two 6 passes from peristaltic pump 7's pump head inside, through setting up peristaltic pump 7, utilize peristaltic pump 7 to carry the cell culture liquid of conveyer pipe two 6 inside, cell culture liquid is in the pipeline all the time and carries, avoid cell culture liquid to spill outside, and reduce the possibility of cell pollution, the one end that conveyer pipe two 6 kept away from electromagnetism three-way valve 5 is connected with fluid replacement head 8, fluid replacement head 8 installs in workstation 1's outside.
Referring to fig. 1, 4 and 5, the suspension mechanism includes a support frame 9 fixed on the top of the workbench 1, two suspension mechanisms are respectively located above the infusion heads 3, two suspension mechanisms are arranged to simultaneously convey cell culture fluid to the two infusion heads 3, the bottom of the support frame 9 is connected with a carriage 10, the bottom of the carriage 10 is clamped with a conveying belt 11, the conveying belt 11 is arranged to convey the cell culture fluid by using the conveying belt 11, no manual supplement is needed, a driving rack 12 is connected to the inner side of the conveying belt 11, a driving motor 13 is installed on the top of the support frame 9, an output shaft of the driving motor 13 movably penetrates through the support frame 9, a driving wheel 14 is connected to the bottom of an output shaft of the driving motor 13, the driving wheel 14 is driven to rotate by the driving motor 13, the conveying belt 11 can be driven to move along the carriage 10, the conveying belt 11 conveys the subsequent bagged cell culture fluid to the infusion heads 3, the driving wheel 14 can be automatically supplemented with the cell culture fluid, and the driving wheel 14 is meshed with the driving rack 12.
Referring to fig. 4 and 6, the bottom of the conveyor belt 11 is connected with a culture solution hanging rack 15, the top of the culture solution hanging rack 15 is connected with a hook 16, the hook 16 is used for hanging bagged cell culture solution through the hook 16, the bagged cell culture solution is conveniently fixed, the bottom of the culture solution hanging rack 15 is connected with a positioning plate 17, a limiting groove 18 is formed in the positioning plate 17, and the limiting groove 18 is arranged to fix the bag mouth of the bagged cell culture solution, so that the bag mouth is prevented from moving.
In summary, when the liquid replenishing device for cell culture is used, the bagged cell culture liquid is suspended on the hook 16, the bag opening at the bottom of the bag is fixed in the limiting groove 18, then the liquid replenishing head 8 is inserted into a cell culture vessel, the liquid feeding head 3 is pushed to move upwards by the air cylinder 2, the liquid feeding head 3 is inserted into the cell culture liquid, then the peristaltic pump 7 is started to convey the cell culture liquid into the cell culture vessel, when one bag of cell culture liquid is used up, the electromagnetic three-way valve 5 switches the conveying pipe II 6 to be communicated with the other liquid feeding head 3, when the two bags of cell culture liquid are used up, the air cylinder 2 drives the liquid feeding head 3 to move downwards to separate from the culture liquid, then the driving motor 13 drives the driving wheel 14 to rotate, the conveying belt 11 is driven to move along the carriage 10, the subsequent bagged cell culture liquid is conveyed to the upper part of the liquid feeding head 3, and then the steps are repeated to replenish the liquid.
In the description of the present utility model, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are based on directions or positional relationships shown in the drawings, are merely for convenience of description and simplification of description, and do not indicate or imply that the apparatus or element to be referred to must have a specific direction, be constructed and operated in the specific direction, and thus should not be construed as limiting the present utility model; the terms "first," "second," "third," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "coupled," and the like are to be construed broadly, and may be fixedly coupled, detachably coupled, or integrally coupled, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a cell culture is with fluid infusion device, includes workstation (1), transfusion system, suspension mechanism, its characterized in that: the infusion mechanism comprises an air cylinder (2) fixed at the top of a workbench (1), an infusion head (3) is mounted at the top of the air cylinder (2), a conveying pipe I (4) is connected to the bottom of the infusion head (3), an electromagnetic three-way valve (5) is connected to the bottom of the conveying pipe I (4), a conveying pipe II (6) is connected to the bottom of the electromagnetic three-way valve (5), a peristaltic pump (7) is mounted in the workbench (1), and the suspension mechanism comprises a supporting frame (9) fixed at the top of the workbench (1).
2. The fluid replacement device for cell culture according to claim 1, wherein: the number of the infusion heads (3) is two, the two infusion heads are symmetrically distributed about the electromagnetic three-way valve (5), and the two infusion heads (3) are connected with an input port of the electromagnetic three-way valve (5) through a first conveying pipe (4).
3. The fluid replacement device for cell culture according to claim 1, wherein: the second conveying pipe (6) penetrates through the pump head of the peristaltic pump (7), one end, far away from the electromagnetic three-way valve (5), of the second conveying pipe (6) is connected with a liquid supplementing head (8), and the liquid supplementing head (8) is arranged on the outer side of the workbench (1).
4. The fluid replacement device for cell culture according to claim 1, wherein: the number of the suspension mechanisms is two, and the two suspension mechanisms are respectively positioned above the infusion head (3).
5. The fluid replacement device for cell culture according to claim 1, wherein: the bottom of support frame (9) is connected with balladeur train (10), the bottom joint of balladeur train (10) has conveyer belt (11), the inboard of conveyer belt (11) is connected with drive rack (12), the bottom of conveyer belt (11) is connected with culture solution stores pylon (15).
6. The fluid replacement device for cell culture according to claim 5, wherein: the top of support frame (9) is installed driving motor (13), the output shaft activity of driving motor (13) passes support frame (9), the bottom of driving motor (13) output shaft is connected with drive wheel (14), drive wheel (14) and drive rack (12) meshing are in the same place.
7. The fluid replacement device for cell culture according to claim 5, wherein: the top of culture solution stores pylon (15) is connected with couple (16), the bottom of culture solution stores pylon (15) is connected with locating plate (17), limiting groove (18) have been seted up to the inside of locating plate (17).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321950134.0U CN220467977U (en) | 2023-07-24 | 2023-07-24 | Cell culture is with fluid infusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321950134.0U CN220467977U (en) | 2023-07-24 | 2023-07-24 | Cell culture is with fluid infusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220467977U true CN220467977U (en) | 2024-02-09 |
Family
ID=89797336
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321950134.0U Active CN220467977U (en) | 2023-07-24 | 2023-07-24 | Cell culture is with fluid infusion device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220467977U (en) |
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2023
- 2023-07-24 CN CN202321950134.0U patent/CN220467977U/en active Active
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