CN220231752U - Portable colloidal gold immunochromatography test paper quantitative determination equipment - Google Patents
Portable colloidal gold immunochromatography test paper quantitative determination equipment Download PDFInfo
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- CN220231752U CN220231752U CN202321963243.6U CN202321963243U CN220231752U CN 220231752 U CN220231752 U CN 220231752U CN 202321963243 U CN202321963243 U CN 202321963243U CN 220231752 U CN220231752 U CN 220231752U
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- colloidal gold
- test paper
- mcu
- immunochromatographic test
- gold immunochromatographic
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- 238000012360 testing method Methods 0.000 title claims abstract description 62
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 59
- 238000003317 immunochromatography Methods 0.000 title claims description 11
- 238000001514 detection method Methods 0.000 claims abstract description 56
- 238000010438 heat treatment Methods 0.000 claims abstract description 23
- 238000012545 processing Methods 0.000 claims abstract description 20
- 238000004891 communication Methods 0.000 claims description 8
- 238000013461 design Methods 0.000 abstract description 4
- 241000700605 Viruses Species 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 11
- 230000008569 process Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 238000009529 body temperature measurement Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 125000000205 L-threonino group Chemical group [H]OC(=O)[C@@]([H])(N([H])[*])[C@](C([H])([H])[H])([H])O[H] 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013135 deep learning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
Abstract
The utility model discloses portable colloidal gold immunochromatographic test paper quantitative detection equipment, and relates to colloidal gold detection equipment. Comprises a machine body, a slot arranged at one side of the machine body and used for being inserted with colloidal gold immunochromatographic test paper, and a processing system arranged in the machine body; the processing system comprises an MCU, a CCD camera, an SD card and an LED lighting module which are electrically connected with the MCU, an infrared heating module for heating the colloidal gold immunochromatographic test paper, and a thermocouple for detecting the temperature of the colloidal gold immunochromatographic test paper; the trigger end of the infrared heating module is electrically connected with the control end of the MCU, and the data output end of the thermocouple is electrically connected with the input end of the MCU. The utility model realizes the heating and temperature detection of the colloidal gold immunochromatographic test paper, is easy to carry, simple in design and simple in use, and is convenient to popularize and apply in areas with insufficient equipment and storage conditions and in areas with virus pandemics.
Description
Technical Field
The utility model relates to colloidal gold detection equipment, in particular to portable colloidal gold immunochromatography test paper quantitative detection equipment.
Background
The colloidal gold immunochromatography detection method has high rate, is quick to detect and simple to operate, and quantitatively detects the concentration of the substance solution to be detected by utilizing the combination mode of the antigen and the antibody, so that the method has become a main detection technical means in the current medical direction.
The existing colloidal gold immunochromatography detection technology mostly adopts a CCD imaging technology to detect colloidal gold immunochromatography test paper, and the technical method is that the CCD is used for collecting the image information of the test paper, and then a computer is used for processing and analyzing by using an image processing technology. However, the existing detection instrument using the technology has the problems of long detection time, high instrument cost, large instrument and the like, so how to design a light instrument system capable of rapidly detecting the attribute and concentration information of the colloidal gold immunochromatography test paper (14) becomes a research hot spot.
The portable CCD reader for colloidal gold disclosed in CN104836942A has the advantages that when test paper detection is carried out, a detection card is inserted into a sample tank, a CCD camera is used for collecting test paper images, an image processing module is used for carrying out image analysis processing, the processed data are transmitted into a single chip microcomputer, the processed data are compared with a standard curve to obtain the measured concentration, and then the measured concentration is output through a display screen. The instrument has the advantages of light structure, suitability for carrying, accurate detection data and capability of overcoming the problem that a large-sized detection instrument is heavy and inconvenient to move.
However, existing portable detection devices can only obtain the concentration of a sample. Experiments prove that the concentrations of the samples on the test paper are different, and the temperatures after heating are different. Through the temperature difference between the heated samples, the detection result can be further judged and checked, so that the accuracy of the detection result is improved. Based on the above, we provide a portable colloidal gold immunochromatography test paper quantitative detection device with the functions of heating a sample and detecting temperature.
Disclosure of Invention
The utility model aims to solve the technical problem of providing a portable colloidal gold immunochromatographic test paper quantitative detection device aiming at the defects of the prior art.
The utility model relates to portable colloidal gold immunochromatographic test paper quantitative detection equipment, which comprises a machine body, a slot arranged on one side of the machine body and used for being inserted with colloidal gold immunochromatographic test paper, and a processing system arranged in the machine body; the processing system comprises an MCU, a CCD camera, an SD card and an LED lighting module which are electrically connected with the MCU, an infrared heating module for heating the colloidal gold immunochromatographic test paper, and a thermocouple for detecting the temperature of the colloidal gold immunochromatographic test paper; the trigger end of the infrared heating module is electrically connected with the control end of the MCU, and the data output end of the thermocouple is electrically connected with the input end of the MCU.
The processing system further comprises a Bluetooth communication module, and the Bluetooth communication module is electrically connected with the MCU.
The machine body is provided with a touch screen, and the touch screen is electrically connected with the MCU.
The CCD camera adopts a 0V7725 camera module.
The thermocouple adopts a MAX6675 module.
The steering engine is arranged in the engine body, the control end of the steering engine is electrically connected with the MCU, the transverse rod is arranged at the driving end of the steering engine, and the thermocouple is arranged at the tail end of the transverse rod.
The machine body is of a square box-packed structure.
Advantageous effects
The utility model has the advantages that:
1. an infrared heating module for heating the colloidal gold immunochromatographic test paper and a thermocouple for detecting the temperature of the colloidal gold immunochromatographic test paper are additionally arranged in a processing system arranged in a machine body, so that the heating and the temperature detection of the colloidal gold immunochromatographic test paper are realized, further judgment and detection of detection results are facilitated, and the accuracy of the detection results is improved.
2. The machine body adopts a box-packed structure, is beneficial to the design of smaller volume, is easy to carry, has simple design and simple use, and is convenient to popularize and apply in areas with insufficient equipment and storage conditions and in virus pandemic areas.
3. Through the setting of the Bluetooth communication module, the remote transmission between the detection equipment and the mobile terminal or the PC terminal is realized.
Drawings
FIG. 1 is a schematic view of the appearance of a detecting apparatus according to the present utility model;
FIG. 2 is a schematic diagram of a processing system according to the present utility model;
FIG. 3 is a schematic diagram of the workflow of the detection apparatus of the present utility model;
FIG. 4 is a flow chart of the test device initialization and self-test process of the present utility model;
fig. 5 is a flowchart of a specific implementation of the detection device of the present utility model.
Detailed Description
The utility model is further described below in connection with the examples, which are not to be construed as limiting the utility model in any way, but rather as falling within the scope of the claims.
Referring to fig. 1-5, the portable colloidal gold immunochromatographic test paper quantitative detection device of the present utility model comprises a machine body 12, a slot 13 arranged at one side of the machine body 12 and used for inserting the colloidal gold immunochromatographic test paper 14, and a processing system arranged in the machine body 12. The machine body 12 is a square box structure. The processing system comprises an MCU, a CCD camera 5 electrically connected with the MCU, an SD card 7, an LED illumination module 3, an infrared heating module 2 and a thermocouple 6.
Wherein, the MCU adopts STM32C8T6 singlechip as main control chip to integrate and control each module, and simultaneously performs image acquisition of the colloidal gold immunochromatographic test paper 14 and identification of quality control line/detection line of the colloidal gold immunochromatographic test paper 14. Since the specific processing procedure of the MCU is the prior art, no further discussion is made herein. The CCD camera 5 adopts a 0V7725 camera module to collect images of the colloidal gold immunochromatographic test paper 14 inserted in the slot 13. The SD card 7 is used for storing the acquired image and temperature value. The LED illumination module 3 is used for illuminating the colloidal gold immunochromatographic test paper 14. The infrared heating module 2 is used for heating the colloidal gold immunochromatographic test paper 14. The MCU controls the power supply of the infrared heating module 2 through the relay module to stop/start the work of the infrared heating module 2. The thermocouple 6 is used to detect the temperature of the colloidal gold immunochromatographic test strip 14 and transmit the temperature to the MCU.
In this embodiment, the MAX6675 module is adopted as the thermocouple 6, and the power supply end thereof is electrically connected with the other control end of the MCU through the relay module. The concrete installation mode is that a steering engine is installed in the engine body 12, the control end of the steering engine is electrically connected with the MCU, the driving end of the steering engine is provided with a cross rod, and the thermocouple 6 is installed at the tail end of the cross rod.
After the MCU will send temperature measurement signal to the control module of steering wheel, the steering wheel starts, and the drive end of steering wheel drives the horizontal pole to drive thermocouple 6 and rotates to the settlement position, thermocouple 6 and the contact of colloidal gold immunochromatography test paper 14 this moment. Meanwhile, the MCU sends out a starting signal to enable the thermocouple 6 to work in a power-on mode, and therefore the temperature of the colloidal gold immunochromatographic test paper 14 is collected. After the detection equipment finishes detecting the colloidal gold immunochromatographic test paper 14, the steering engine and the thermocouple 6 are reset.
Preferably, the processing system further includes a bluetooth communication module 9, where the bluetooth communication module 9 is electrically connected to the MCU, and is configured to perform remote communication with the mobile terminal 10 or the PC terminal 11, so as to implement mutual transmission of data.
Preferably, the body 12 is provided with a touch screen 8, and the touch screen 8 is electrically connected with the MCU and is used for displaying the acquired image and the detection data generated in the detection process.
The detection device is further provided with a power supply module 1 for supplying power. The power module 1 provides power for the MCU and the module needing independent power supply, and the power module 1 provides output of 5V and 3.3V two kinds of voltages, so that different requirements of different modules on voltage input are met.
The working principle of the utility model is as follows: in use, the colloidal gold immunochromatographic test paper 14 is inserted into the slot 13 of the detection device. As shown in fig. 3, which is a working flow chart of the detection device, after the detection device is powered on, the detection device automatically detects whether the colloidal gold immunochromatographic test paper 14 is in the slot 13. If the colloidal gold immunochromatographic test strip 14 is present, the C/T line of the colloidal gold immunochromatographic test strip 14 is captured, and then the image and/or the temperature of the colloidal gold immunochromatographic test strip 14 is acquired and quantified. And finally, outputting the quantized result to a display screen. The capturing of the C/T line and the quantification of the acquired data are all the prior art.
The specific operation is shown in fig. 4-5. After the detection equipment is powered on, the detection equipment performs initialization and self-checking of each module, such as SD card self-checking, LCD touch screen self-checking, CCD camera self-checking, thermocouple self-checking and the like, if the self-checking has error reporting, the LCD touch screen can have error reporting dialog box to prompt the self-checking to have error reporting, whether each module is powered on is firstly confirmed, after the power on is confirmed to be correct, a self-checking button on the touch screen is clicked to perform re-self-checking again, and the specific initialization and self-checking process is shown in fig. 4. Since the initialization and self-checking process is an original function in the detection device, the present utility model does not improve the same, and thus, the description thereof is omitted.
After the automatic initialization of the detection equipment is finished, the operation of quantitatively detecting the colloidal gold immunochromatographic test paper 14 can be carried out only by carrying out the operation according to the prompt of the interactive interface after the human-computer interactive interface is displayed on the touch screen 8.
Specifically, fig. 5 is a flowchart of a specific implementation of the detection device in detection. Three modes, namely an image acquisition mode, a temperature acquisition mode and a mixed mode, are provided on the touch screen 8, and the three modes on the touch screen 8 are selected for testing.
For example, when the image acquisition mode is adopted, the detection of the original detection mode of the apparatus is performed. The LED illumination is started, the CCD camera 5 is started, the image is processed by the MCU after being acquired, and the processing result is transmitted to the touch screen 8 for display. The method comprises the steps that an image is acquired, images monitored in real time by a deep learning target detection method are compared with a colloidal gold immunochromatographic test paper data set trained by yolov5, 5000 600 x 640 pictures of various common colloidal gold immunochromatographic test papers on the market are used as training samples in the colloidal gold immunochromatographic test paper data set, and all common colloidal gold immunochromatographic test papers on the market can be identified; the redundant candidates are optimized by using NMS algorithm in the process of obtaining the image, and non-maximal suppression is carried out on all boxes (positioning samples) obtained by the model in the process of testing and detecting:
(1) screening boxes meeting confidence threshold (conf_thres) condition
(2) Screening out the most probable class of each box from (1) as the most probable class of the box
(3) Coordinate conversion: box (x, y, w, h) - > box (x 1, y1, x2, y 2)
(4) And (3) sorting information (x 1, y1, x2, y2, confidence, probability and class) of the remaining boxes after screening, and counting non-repeated classes in all boxes.
(5) Traversing the class, taking corresponding boxes of each traversing class, calculating score=confidence degree probability of the boxes, sorting the scores, recording original box positions (line numbers) after sorting, and re-acquiring the sorted boxes.
(6) NMS processing: firstly, the box corresponding to the maximum score is stored, and then the iou of the box and all the boxes after the box is calculated (two boxes of the same type)
(7) Satisfying iou > nms_thres (predicting that a plurality of boxes are reserved in the same position), recording the serial numbers of the boxes, temporarily eliminating the boxes, performing (6) and (7) operations on the rest boxes, and finally obtaining unique boxes in different positions (the position predictions are all of the same type)
(8) Traversing the next class: repeating (5) (6) (7), and finally screening out unique boxes of the same type or different types at different positions.
The training amount of the program is sufficient, the parameter conf-thres in the program is set to 0.8, the iou-thres is set to 0.5, so that the detection precision is improved, the number of the colloidal gold immunochromatographic test papers in the picture is generally only 1, and the max-det is set to 1.
The colloidal gold immunochromatographic test paper data set trained by the algorithm is imported into the MCU as a program for collecting images, the algorithm captures images containing marked samples and then intercepts the images for calculation and uploading, and the method improves the recognition accuracy and recognition speed and obtains needed information more quickly.
When the temperature acquisition mode is adopted, the MCU sends out a heating instruction to enable the infrared heating module 2 to heat the colloidal gold immunochromatographic test paper 14. After heating, the MCU sends out a temperature measurement instruction, and the steering engine drives the thermocouple 6 measuring end to move to the detection line of the colloidal gold immunochromatographic test paper 14, and the temperature measurement is carried out through the thermocouple 6. After the measurement is finished, the steering engine drives the thermocouple 6 to return to the initial position in the original path. When the thermocouple collects temperature data, the collected temperature data adopts median filtering, so that temperature fluctuation caused by accidental factors can be effectively overcome.
When the mixed acquisition mode is adopted, the detection equipment is firstly carried out in the flow of the image acquisition mode, and then is carried out in the flow of the temperature acquisition mode.
While only the preferred embodiments of the present utility model have been described above, it should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present utility model, and these do not affect the effect of the implementation of the present utility model and the utility of the patent.
Claims (7)
1. A portable colloidal gold immunochromatographic test paper quantitative detection device comprises a machine body (12), a slot (13) arranged on one side of the machine body (12) and used for being inserted with colloidal gold immunochromatographic test paper (14), and a processing system arranged in the machine body (12); the processing system comprises an MCU, a CCD camera (5) electrically connected with the MCU, an SD card (7) and an LED lighting module (3), and is characterized by further comprising an infrared heating module (2) for heating the colloidal gold immunochromatography test paper (14) and a thermocouple (6) for detecting the temperature of the colloidal gold immunochromatography test paper (14); the trigger end of the infrared heating module (2) is electrically connected with the control end of the MCU, and the data output end of the thermocouple (6) is electrically connected with the input end of the MCU.
2. The portable colloidal gold immunochromatographic test strip quantitative detection device according to claim 1, wherein the processing system further comprises a Bluetooth communication module (9), and the Bluetooth communication module (9) is electrically connected with the MCU.
3. The portable colloidal gold immunochromatographic test strip quantitative detection device according to claim 1, wherein the machine body (12) is provided with a touch screen (8), and the touch screen (8) is electrically connected with the MCU.
4. The portable colloidal gold immunochromatographic test strip quantitative detection device according to claim 1, wherein the CCD camera (5) adopts a 0V7725 camera module.
5. The portable colloidal gold immunochromatographic test strip quantitative detection device according to claim 1, wherein the thermocouple (6) adopts a MAX6675 module.
6. The portable colloidal gold immunochromatographic test paper quantitative detection device according to claim 1 or 5, wherein a steering engine is installed in the machine body (12), a control end of the steering engine is electrically connected with the MCU, a cross rod is installed at a driving end of the steering engine, and the thermocouple (6) is installed at the tail end of the cross rod.
7. The portable colloidal gold immunochromatographic test strip quantitative detection device of claim 1, wherein the body (12) has a square box-packed structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321963243.6U CN220231752U (en) | 2023-07-25 | 2023-07-25 | Portable colloidal gold immunochromatography test paper quantitative determination equipment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321963243.6U CN220231752U (en) | 2023-07-25 | 2023-07-25 | Portable colloidal gold immunochromatography test paper quantitative determination equipment |
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| Publication Number | Publication Date |
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| CN220231752U true CN220231752U (en) | 2023-12-22 |
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|---|---|---|---|
| CN202321963243.6U Active CN220231752U (en) | 2023-07-25 | 2023-07-25 | Portable colloidal gold immunochromatography test paper quantitative determination equipment |
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| CN (1) | CN220231752U (en) |
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- 2023-07-25 CN CN202321963243.6U patent/CN220231752U/en active Active
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