CN220166171U - Cell culture dish capable of avoiding pollution - Google Patents
Cell culture dish capable of avoiding pollution Download PDFInfo
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- CN220166171U CN220166171U CN202321454504.1U CN202321454504U CN220166171U CN 220166171 U CN220166171 U CN 220166171U CN 202321454504 U CN202321454504 U CN 202321454504U CN 220166171 U CN220166171 U CN 220166171U
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- cell culture
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- culture medium
- dish
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- 238000004113 cell culture Methods 0.000 title claims abstract description 28
- 239000001963 growth medium Substances 0.000 claims abstract description 48
- 230000007246 mechanism Effects 0.000 claims abstract description 31
- 230000004888 barrier function Effects 0.000 claims abstract description 12
- 238000012795 verification Methods 0.000 claims abstract description 11
- 238000012544 monitoring process Methods 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims description 17
- 238000012360 testing method Methods 0.000 claims description 17
- 238000011109 contamination Methods 0.000 claims description 4
- 238000003556 assay Methods 0.000 claims 1
- 238000012806 monitoring device Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of cell culture equipment, and discloses a pollution-free cell culture dish which comprises a sterile table and further comprises: the utility model discloses a temperature and humidity monitoring device, which is characterized in that a barrier strip is fixedly arranged on a diagonal line of the outer wall of the top of a sterile table, the sterile table is divided into two areas by the barrier strip, the surfaces of the two areas are respectively provided with a culture mechanism and a verification mechanism, a first circular groove and a second circular groove are formed in the outer wall of the top of the sterile table, the culture mechanism and the verification mechanism are respectively arranged in the first circular groove and the second circular groove, the culture mechanism comprises a culture medium and a dish cover, and a monitoring mechanism is arranged on the outer wall of one side of the culture medium.
Description
Technical Field
The utility model relates to the technical field of cell culture equipment, in particular to a cell culture dish capable of avoiding pollution.
Background
Cell culture refers to a method of simulating in vitro an in vivo environment (sterile, suitable temperature, ph, certain nutritional conditions, etc.) to survive, grow, reproduce and maintain the major structure and function, and requires the use of culture dishes for cell culture.
In prior art (CN 207998615U, chinese patent application entitled cell culture dish), including outer dish body, a plurality of interior dish body and lid, the lid is located on the outer dish body, and outer dish body is connected with a plurality of vertical first baffles that set up, and first baffle separates into a plurality of holding chambers with outer dish body, and interior dish body detachable is located the holding intracavity, and the device can change the culture dish under the same cultivation condition or different time batch culture, and can reduce the pollution probability of culture dish. In the process of realizing the technical scheme, the inventor finds that at least the following problems exist in the prior art:
in practical application, when the device is used, the culture dish is divided into a plurality of chambers, but the whole culture dish is easily polluted if the culture dish is polluted, so that the culture of cells is influenced, and therefore, a cell culture dish capable of avoiding pollution is needed to solve the problems.
Disclosure of Invention
The utility model aims to provide a cell culture dish capable of avoiding pollution, which aims to solve the problem that the whole culture dish is polluted when the culture dish is polluted in the same way as a plurality of chambers in the culture dish provided in the prior art.
In a first aspect, the utility model provides a contamination-protected cell culture dish comprising a sterile stand, further comprising: a barrier strip is fixedly arranged on a diagonal line of the outer wall of the top of the sterile table, the sterile table is divided into two areas by the barrier strip, the surfaces of the two areas are provided with a culture mechanism and a verification mechanism, a first circular groove and a second circular groove are arranged on the outer wall of the top of the sterile table, the culture mechanism and the verification mechanism are respectively arranged in the first circular groove and the second circular groove, the culture mechanism comprises a culture medium and a dish cover, and a monitoring mechanism is arranged on the outer wall of one side of the culture medium.
In a specific embodiment, a non-slip mat is adhered to the bottom outer wall of the sterile table.
In a specific embodiment, the culture medium is clamped on the inner wall of the first circular groove, an annular check ring is clamped on the outer side of the culture medium, and the annular check ring is fixedly installed on the top outer wall of the sterile table.
In a specific embodiment, the top outer wall of the culture medium is provided with an annular groove.
In a specific embodiment, the annular clamping strips are fixedly installed on the outer wall of the bottom of the dish cover and are clamped on the inner wall of the corresponding annular groove, and armrests are fixedly installed on the outer walls of the two sides of the dish cover.
In a specific embodiment, the monitoring mechanism comprises a connecting seat, wherein the connecting seat is fixedly arranged on one side outer wall of the culture medium, a mounting hole is formed in one side outer wall of the connecting seat, a sealing tube is inserted into the circumferential inner wall of the mounting hole, and a temperature and humidity monitor is inserted into the circumferential inner wall of the sealing tube.
In a specific embodiment, a circular hole is formed in the outer wall of one side of the culture medium, close to the connecting seat, and one end of the temperature and humidity monitor penetrates through the circular hole to be located in the culture medium.
In a specific embodiment, a sealing plug is clamped on the outer wall of the top of the connecting seat, and an elastic ring is sleeved on the outer wall of the circumference of the sealing plug.
In a specific embodiment, the verification mechanism comprises a test box, the test box is clamped in the second circular groove, and a cover plate is clamped on the outer wall of the top of the test box.
In a specific embodiment, the test cassette is a sterile culture plate.
According to the cell culture dish capable of avoiding pollution, the culture mediums are placed on two sides of a diagonal line of the sterile table, the two culture mediums are separated through the barrier strips, the two culture mediums are subjected to identical culture, comparison tests are convenient to conduct, meanwhile, under the condition that one culture medium is polluted, the other culture medium is used for culture, the temperature and the humidity in the culture medium are monitored through the temperature and humidity monitor, so that the temperature and humidity in the culture medium are in a proper temperature and humidity range, and when whether the culture medium is polluted or not needs to be judged later, the culture is conducted through sampling of the sterile culture flat plate, and if a colony exists, the pollution is indicated.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic overall structure of an embodiment of the present utility model.
Fig. 2 is a schematic view of a structure of a sterile table according to an embodiment of the present utility model.
Fig. 3 is a schematic structural diagram of a monitoring mechanism according to an embodiment of the present utility model.
FIG. 4 is a schematic diagram of a culture medium according to an embodiment of the present utility model.
Fig. 5 is a schematic view of a dish cover according to an embodiment of the utility model.
Icon: 1. a sterile station; 2. a barrier strip; 3. a temperature and humidity monitor; 4. an annular retainer ring; 5. an armrest; 6. a dish cover; 7. a culture medium; 8. a cover plate; 9. a test box; 10. an anti-slip pad; 11. a first circular groove; 12. a second circular groove; 13. sealing the tube; 14. a connecting seat; 15. a sealing plug; 16. an elastic ring; 17. a mounting hole; 18. an annular groove; 19. a circular hole; 20. an annular clamping strip.
Detailed Description
Because the culture dish is divided into a plurality of chambers in the prior art, but the culture dish is polluted in the same step, if the culture dish is polluted, the whole culture dish is polluted easily. Thus, the inventors studied to provide a cell culture dish avoiding contamination, in which two media are separated by a barrier strip, the two media are cultured identically, and a comparative experiment is facilitated, while in the case that one of the media is contaminated, the culture can be performed through the other media, thereby solving the above-mentioned drawbacks.
Some embodiments of the present utility model are described in detail below with reference to the accompanying drawings. The following embodiments and features of the embodiments may be combined with each other without conflict.
Referring to fig. 1 to 5, an embodiment of the present utility model provides a cell culture dish for avoiding contamination, comprising a sterile table 1, further comprising: the barrier strip 2 is fixedly installed on a diagonal line of the outer wall of the top of the sterile table 1, the sterile table 1 is divided into two areas by the barrier strip 2, the surfaces of the two areas are provided with a culture mechanism and a verification mechanism, the outer wall of the top of the sterile table 1 is provided with a first circular groove 11 and a second circular groove 12, the culture mechanism and the verification mechanism are respectively arranged in the first circular groove 11 and the second circular groove 12, the culture mechanism comprises a culture medium 7 and a dish cover 6, and a monitoring mechanism is arranged on the outer wall of one side of the culture medium 7.
Further, the anti-slip pad 10 is adhered to the outer wall of the bottom of the aseptic platform 1, and the aseptic platform 1 is more stable through the arrangement of the anti-slip pad 10.
Further, culture medium 7 joint is on the inner wall of first circular slot 11, and culture medium 7's outside joint has annular retaining ring 4, and annular retaining ring 4 fixed mounting is on aseptic technique 1's top outer wall, carries out spacingly to culture medium 7 through annular retaining ring 4, guarantees that culture medium 7 is in steady state, the cell culture's of being convenient for goes on.
Further, an annular groove 18 is formed in the top outer wall of the culture medium 7.
Further, the annular clamping strips 20 are fixedly installed on the outer wall of the bottom of the dish cover 6, the annular clamping strips 20 are clamped on the inner wall of the corresponding annular groove 18, the handrails 5 are fixedly installed on the outer walls of the two sides of the dish cover 6, the dish cover 6 is conveniently attached to the surface of the culture medium 7 through the annular clamping strips 20, and the dish cover 6 is conveniently taken down through the handrails 5.
Further, the monitoring mechanism comprises a connecting seat 14, the connecting seat 14 is fixedly arranged on the outer wall of one side of the culture medium 7, a mounting hole 17 is formed in the outer wall of one side of the connecting seat 14, a sealing tube 13 is inserted into the circumferential inner wall of the mounting hole 17, a temperature and humidity monitor 3 is inserted into the circumferential inner wall of the sealing tube 13, and the temperature and humidity inside the culture medium 7 can be conveniently monitored through the temperature and humidity monitor 3.
Further, a circular hole 19 is formed in the outer wall of one side of the culture medium 7 and close to the connecting seat 14, and one end of the temperature and humidity monitor 3 penetrates through the circular hole 19 and is located in the culture medium 7.
Further, the sealing plug 15 is clamped on the top outer wall of the connecting seat 14, the elastic ring 16 is sleeved on the circumferential outer wall of the sealing plug 15, the sealing tube 13 can be plugged through the sealing plug 15 when the connecting seat stands still, and the sealing tube 13 is clamped through the elastic ring 16, so that air tightness is ensured.
Further, the verification mechanism comprises a testing box 9, the testing box 9 is clamped in the second circular groove 12, and the cover plate 8 is clamped on the outer wall of the top of the testing box 9.
Further, the test box 9 is a sterile culture plate, and the detection of whether the culture medium 7 is polluted or not is facilitated by the arranged sterile culture plate.
To sum up, a cell culture dish avoiding pollution is characterized in that two culture mediums 7 are separated by a barrier strip 2, the two culture mediums 7 are cultured in the same way, a comparison test is convenient to carry out, and meanwhile, under the condition that one culture medium 7 is polluted, the other culture medium 7 can be used for culturing.
Working principle: after the aseptic platform 1, the culture medium 7 and the test box 9 are disinfected and sterilized, the culture medium 7 and the test box 9 are placed on the surface of the aseptic platform 1, then cell culture is carried out through the culture medium 7, the dish cover 6 is covered on the surface of the culture medium 7 through the handrails 5, then culture is started, the temperature and humidity inside the culture medium 7 are monitored through the temperature and humidity monitor 3, the two culture mediums 7 are subjected to the same culture, a comparison test is convenient, meanwhile, under the condition that one culture medium 7 is polluted, culture can be carried out through the other culture medium 7, and when whether the culture medium 7 is polluted or not is judged later, culture is carried out through sampling of an aseptic culture flat plate, and if a colony exists, the occurrence of pollution is indicated.
The above is only a preferred embodiment of the present utility model, and is not intended to limit the present utility model, but various modifications and variations can be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (10)
1. A cell culture dish for avoiding contamination comprising a sterile station, further comprising: a barrier strip is fixedly arranged on a diagonal line of the outer wall of the top of the sterile table, the sterile table is divided into two areas by the barrier strip, the surfaces of the two areas are provided with a culture mechanism and a verification mechanism, a first circular groove and a second circular groove are arranged on the outer wall of the top of the sterile table, the culture mechanism and the verification mechanism are respectively arranged in the first circular groove and the second circular groove, the culture mechanism comprises a culture medium and a dish cover, and a monitoring mechanism is arranged on the outer wall of one side of the culture medium.
2. A cell culture dish as claimed in claim 1, wherein the bottom outer wall of the sterile table is bonded with a non-slip mat.
3. The cell culture dish for avoiding pollution according to claim 1, wherein the culture medium is clamped on the inner wall of the first circular groove, an annular retainer ring is clamped on the outer side of the culture medium, and the annular retainer ring is fixedly arranged on the top outer wall of the sterile table.
4. A cell culture dish according to claim 3 wherein the outer wall of the top of the culture medium is provided with an annular groove.
5. The cell culture dish for avoiding pollution according to claim 4, wherein the outer wall of the bottom of the dish cover is fixedly provided with an annular clamping strip, the annular clamping strip is clamped on the inner wall of the corresponding annular groove, and the outer walls of the two sides of the dish cover are fixedly provided with handrails.
6. The cell culture dish for avoiding pollution according to claim 1, wherein the monitoring mechanism comprises a connecting seat, the connecting seat is fixedly installed on the outer wall of one side of the culture medium, a mounting hole is formed in the outer wall of one side of the connecting seat, a sealing tube is inserted into the circumferential inner wall of the mounting hole, and a temperature and humidity monitor is inserted into the circumferential inner wall of the sealing tube.
7. The cell culture dish for avoiding pollution according to claim 6, wherein the outer wall of one side of the culture medium is provided with a circular hole near the connecting seat, and one end of the temperature and humidity monitor penetrates through the circular hole to be positioned in the culture medium.
8. The cell culture dish for avoiding pollution according to claim 6, wherein the sealing plug is clamped on the outer wall of the top of the connecting seat, and the elastic ring is sleeved on the outer wall of the circumference of the sealing plug.
9. The cell culture dish according to claim 1, wherein the verification mechanism comprises a test box, the test box is clamped inside the second circular groove, and a cover plate is clamped on the top outer wall of the test box.
10. The contamination free cell culture dish of claim 9, wherein the assay cassette is a sterile culture plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321454504.1U CN220166171U (en) | 2023-06-08 | 2023-06-08 | Cell culture dish capable of avoiding pollution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321454504.1U CN220166171U (en) | 2023-06-08 | 2023-06-08 | Cell culture dish capable of avoiding pollution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220166171U true CN220166171U (en) | 2023-12-12 |
Family
ID=89055967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321454504.1U Active CN220166171U (en) | 2023-06-08 | 2023-06-08 | Cell culture dish capable of avoiding pollution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220166171U (en) |
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2023
- 2023-06-08 CN CN202321454504.1U patent/CN220166171U/en active Active
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