CN220166159U

CN220166159U - Multichannel miniature bioreactor

Info

Publication number: CN220166159U
Application number: CN202321363938.0U
Authority: CN
Inventors: 杨海蛟; 燕佩霞
Original assignee: Jiuquan Youyue Information Technology Co ltd
Current assignee: Jiuquan Youyue Information Technology Co ltd
Priority date: 2023-05-31
Filing date: 2023-05-31
Publication date: 2023-12-12
Anticipated expiration: 2033-05-31

Abstract

The utility model relates to a multichannel micro-bioreactor, which comprises a device bin, wherein the inner bottom wall of the device bin is fixedly connected with a reaction bin, the top surface of the device bin is provided with a cover plate, the top surface of the cover plate is fixedly connected with a plurality of feeding pipes, one ends of the feeding pipes vertically penetrate through the cover plate and extend into the device bin, the outer peripheral walls of the feeding pipes are fixedly provided with first electromagnetic valves, the top surfaces of the feeding pipes are respectively provided with a sealing plate, and the bottom surfaces of the sealing plates are respectively fixedly connected with a sealing pad, one ends of the sealing pad extend into the feeding pipes. This multichannel micro-bioreactor stores different additives through a plurality of inlet pipe effects down, and then under the effect through first solenoid valve, controls the emission of additive, makes things convenient for the staff to use, avoids causing the injury to the microorganism as far as possible, simultaneously, seals the inlet pipe through under the effect of sealed pad, improves the leakproofness of inlet pipe.

Description

Multichannel miniature bioreactor

Technical Field

The utility model relates to the technical field of reactors, in particular to a multichannel micro-bioreactor.

Background

The bioreactor mainly uses naturally-occurring microorganisms to be placed in a liquid phase or a solid phase for reaction so as to culture a required biological material, wherein in the experimental culture of the microorganisms, in order to fully react the microorganisms reacted under the same conditions, a multi-channel micro-bioreactor is needed to be used for reaction of a plurality of groups of microorganisms.

The patent CN208562359U discloses a micro-bioreactor with improved ventilation structure, which discloses a technical scheme for improving the gas mass transfer efficiency in the reactor, and solves the problems that the existing method for improving the rotating speed can generate larger shearing force, the high shearing force caused by the high rotating speed can damage cells, and meanwhile, the operating space of the micro-reactor for regulating and controlling oxygen is reduced, so that the application range of the micro-bioreactor is not beneficial to further expanding to support the cell culture with high oxygen consumption and high density.

However, when the device is used, the additive is added through a single pipeline, so that the addition of a plurality of additives by workers is inconvenient, the pipeline is repeatedly opened, the single output of air is caused to form impact on a single position in the culture solution, the impact is further caused on microorganisms in the culture solution, and the microorganisms are damaged, and therefore, the multichannel micro-bioreactor is provided for solving the problems.

Disclosure of Invention

Aiming at the defects of the prior art, the utility model provides the multichannel micro bioreactor which has the advantage of multichannel input, and solves the problems that when the device is used, additives are added through a single pipeline, a worker cannot conveniently add various additives, the pipeline is required to be repeatedly opened, and the single output of air causes the impact of the pipeline on a single position in a culture solution, so that the impact is caused on microorganisms in the culture solution and the damage is caused on the microorganisms.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the multichannel micro bioreactor comprises a device bin, wherein a reaction bin is fixedly connected with the inner bottom wall of the device bin, and a cover plate is arranged on the top surface of the device bin.

The top surface fixedly connected with quantity of apron is a plurality of and one end runs through the apron perpendicularly and extends to the inside inlet pipe of device storehouse, and is a plurality of the equal fixed mounting of periphery wall of inlet pipe has a first solenoid valve, and a plurality of the closing plate has all been placed to the top surface of inlet pipe, a plurality of the equal fixedly connected with one end of bottom surface of closing plate extends to the inside sealed pad of inlet pipe.

And the device bin is provided with a stirring assembly for accelerating the efficiency of the microbial reaction.

The device bin is provided with a flow guide component for discharging objects.

Further, the shape of device storehouse is inside cavity and the cuboid that the top surface lacks, sealed pad is the ring form, and with the laminating of the inner peripheral wall of inlet pipe, a solenoid valve is located the inside of device storehouse.

Further, the bottom surface fixedly connected with solid fixed ring of apron, the groove of stepping down has been seted up to solid fixed ring's bottom surface, the device storehouse extends to the inside of the groove of stepping down, the inside fixedly connected with fixed bolster of groove of stepping down.

Further, the fixing ring and the device bin are tightly attached to the fixing pad.

Further, the stirring assembly comprises a driving motor, the driving motor is fixedly mounted on the top surface of the cover plate, one end of the output end of the driving motor penetrates through the cover plate and extends to a rotating rod on the inner bottom wall of the reaction bin, a limiting hole is formed in the inner bottom wall of the reaction bin, the rotating rod extends to the inside of the limiting hole, stirring rods with a plurality of stirring rods are fixedly connected to the left side surface and the right side surface of the rotating rod, and a connecting rod is vertically fixed between every two adjacent stirring rods.

Further, the shape of reaction storehouse is inside cavity and the cylinder that the top surface lacks, and is a plurality of the puddler all is located the inside of reaction storehouse, is located a plurality of homonymy the puddler is linear equidistance from last down and distributes, bull stick and spacing hole clearance fit.

Further, the water conservancy diversion subassembly includes the water conservancy diversion storehouse, water conservancy diversion storehouse fixed connection is in the inside in device storehouse, water conservancy diversion storehouse and reaction storehouse fixed connection, the bull stick runs through the water conservancy diversion storehouse, equal fixedly connected with supporting leg all around the bottom surface in device storehouse, the interior bottom wall fixedly connected with in reaction storehouse is two and one end runs through the reaction storehouse and extends the row material pipe of device storehouse bottom, two the equal fixed mounting of outer perisporium of row material pipe has the second solenoid valve.

Further, the shape of water conservancy diversion storehouse is inside cavity and the cone that top surface and bottom surface all were missed, two arrange the material pipe and use the bull stick to be the bilateral symmetry distribution as the center, two arrange the bottom relative slope of material pipe.

Compared with the prior art, the technical scheme of the utility model has the following beneficial effects:

1. according to the multichannel micro-bioreactor, different additives are stored under the action of the plurality of feeding pipes, and then the discharge of the additives is controlled under the action of the first electromagnetic valve, so that the multichannel micro-bioreactor is convenient for workers to use, the damage to microorganisms is avoided as much as possible, meanwhile, the feeding pipes are sealed under the action of the sealing gasket, and the sealing performance of the feeding pipes is improved;

2. this multichannel micro-bioreactor, under the effect through the fixed bolster, convenient is fixed the apron, makes things convenient for the staff to dismouting, under the effect through the puddler for the reaction efficiency of microorganism, simultaneously, under the effect through the row material pipe, convenient with microorganism discharge, make things convenient for the staff to operate, convenient and practical more.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is an enlarged schematic view of the inner structure of the feed pipe in the structure of the present utility model;

fig. 3 is a schematic front view of the structure of the present utility model.

In the figure: 1 device bin, 2 reaction bin, 3 water conservancy diversion bin, 4 bull stick, 5 first solenoid valve, 6 inlet pipes, 7 closing plate, 8 driving motor, 9 apron, 10 solid fixed ring, 11 fixed bolster, 12 groove of stepping down, 13 puddler, 14 row material pipe, 15 supporting legs, 16 second solenoid valve, 17 connecting rod, 18 sealing washer, 19 spacing hole.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Embodiment one: referring to fig. 1-3, a multi-channel micro-bioreactor in this embodiment includes a device housing 1, a reaction housing 2 fixedly connected to an inner bottom wall of the device housing 1, and a cover plate 9 disposed on a top surface of the device housing 1.

The top surface fixedly connected with quantity of apron 9 just runs through apron 9 perpendicularly and extends to the inside inlet pipe 6 of device storehouse 1 a plurality of and one end, the equal fixed mounting of periphery wall of a plurality of inlet pipes 6 has first solenoid valve 5, sealing plate 7 has all been placed to the top surface of a plurality of inlet pipes 6, the equal fixedly connected with one end of the bottom surface of a plurality of sealing plates 7 extends to the inside sealed pad 18 of inlet pipe 6, the bottom surface fixedly connected with solid fixed ring 10 of apron 9, the groove 12 of stepping down has been seted up to the bottom surface of solid fixed ring 10, device storehouse 1 extends to the inside of groove 12 of stepping down, the inside fixedly connected with fixed pad 11 of groove 12 of stepping down.

Wherein, device storehouse 1's shape is the cuboid that inside cavity and top surface were absent, and sealing pad 18 is the ring, and laminates with the inner peripheral wall of inlet pipe 6, and first solenoid valve 5 is located the inside of device storehouse 1, and fixed ring 10 and device storehouse 1 all closely laminate with fixed pad 11.

The fixing pad 11 and the sealing pad 18 are both ring-shaped and rubber pads.

Specifically, place microorganism into reaction storehouse 2's inside, place apron 9 at the top surface of device storehouse 1, the inside of the groove 12 of stepping down is entered at the top of device storehouse 1, laminate with fixed pad 11, fix apron 9, pour into the inside of a plurality of inlet pipes 6 respectively with multiple additive, place closing plate 7 on inlet pipe 6, sealed inside that fills up 18 enters into inlet pipe 6, fix apron 9, start a plurality of solenoid valves 5 in proper order, add the inside of reaction storehouse 2 respectively with multiple additive.

Embodiment two: referring to fig. 1-3, on the basis of the first embodiment, a stirring assembly is arranged on a device bin 1, the stirring assembly comprises a driving motor 8, the driving motor 8 is fixedly installed on the top surface of a cover plate 9, an output end of the driving motor 8 is fixedly connected with a rotating rod 4, one end of the rotating rod penetrates through the cover plate 9 and extends to the inner bottom wall of a reaction bin 2, a limiting hole 19 is formed in the inner bottom wall of the reaction bin 2, the rotating rod 4 extends to the inside of the limiting hole 19, stirring rods 13 with a plurality of stirring rods 13 are fixedly connected to the left side surface and the right side surface of the rotating rod 4, and a connecting rod 17 is vertically fixed between two adjacent stirring rods 13.

Wherein, the shape of reaction storehouse 2 is inside cavity and the cylinder that the top surface lacks, and a plurality of puddlers 13 all are located the inside of reaction storehouse 2, and a plurality of puddlers 13 that are located the homonymy are linear equidistance from last down and distribute, bull stick 4 and spacing hole 19 clearance fit.

Specifically, the driving motor 8 is started, and the output end of the driving motor 8 drives the rotating rod 4 to rotate, so as to drive the stirring rod 13 to do circular motion, and the microorganisms are stirred.

Embodiment III: referring to fig. 1-3, on the basis of the first embodiment and the second embodiment, a diversion assembly is arranged on the device bin 1, the diversion assembly comprises a diversion bin 3, the diversion bin 3 is fixedly connected inside the device bin 1, the diversion bin 3 is fixedly connected with the reaction bin 2, the rotating rod 4 penetrates through the diversion bin 3, supporting legs 15 are fixedly connected to the periphery of the bottom surface of the device bin 1, the inner bottom wall of the reaction bin 2 is fixedly connected with two discharge pipes 14, one ends of which penetrate through the reaction bin 2 and extend to the bottom of the device bin 1, and the outer peripheral walls of the two discharge pipes 14 are fixedly provided with second electromagnetic valves 16.

The guide bin 3 is in a hollow cone shape, the top surface and the bottom surface of the cone are missing, the two discharge pipes 14 are symmetrically distributed left and right with the rotary rod 4 as the center, and the bottom ends of the two discharge pipes 14 are inclined relatively.

It should be noted that, the first electromagnetic valve 5 and the second electromagnetic valve 16 are conventional devices known to the public in the prior art, and the specific structure and the working principle thereof will not be described herein.

Specifically, the additive is limited by the action of the diversion bin 3, and after the microorganism reaction is finished, the second electromagnetic valve 16 is started, and the microorganism is discharged by the delivery of the discharge pipe 14.

The working principle of the embodiment is as follows:

the method comprises the steps of placing microorganisms into the reaction bin 2, placing a cover plate 9 on the top surface of the device bin 1, enabling the top of the device bin 1 to enter the inside of a yielding groove 12, attaching a fixing pad 11, fixing the cover plate 9, pouring various additives into the inside of a plurality of feeding pipes 6 respectively, placing a sealing plate 7 on the feeding pipes 6, enabling a sealing pad 18 to enter the inside of the feeding pipes 6, fixing the cover plate 9, sequentially starting a plurality of first electromagnetic valves 5, respectively adding various additives into the inside of the reaction bin 2, limiting the additives under the action of a diversion bin 3, starting a driving motor 8, enabling the output end of the driving motor 8 to drive a rotating rod 4 to rotate, further driving a stirring rod 13 to perform circular motion, stirring the microorganisms, and after the microorganism reaction is finished, starting a second electromagnetic valve 16 to discharge the microorganisms under the conveying of a discharge pipe 14.

The control mode of the utility model is controlled by the controller, the control circuit of the controller can be realized by simple programming by a person skilled in the art, the supply of power also belongs to common knowledge in the art, and the utility model is mainly used for protecting the mechanical device, so the utility model does not explain the control mode and circuit connection in detail.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A multichannel micro-bioreactor comprising a device compartment (1), characterized in that: the inner bottom wall of the device bin (1) is fixedly connected with a reaction bin (2), and a cover plate (9) is arranged on the top surface of the device bin (1);

the top surface of the cover plate (9) is fixedly connected with a plurality of feeding pipes (6) with one ends penetrating through the cover plate (9) vertically and extending to the inside of the device bin (1), the first electromagnetic valves (5) are fixedly installed on the peripheral walls of the feeding pipes (6), sealing plates (7) are arranged on the top surfaces of the feeding pipes (6), and sealing gaskets (18) with one ends extending to the inside of the feeding pipes (6) are fixedly connected to the bottom surfaces of the sealing plates (7);

the device bin (1) is provided with a stirring assembly, and the device bin (1) is provided with a flow guiding assembly.

2. A multi-channel micro-bioreactor according to claim 1, wherein: the shape of device storehouse (1) is inside cavity and the cuboid that the top surface lacks, sealed pad (18) are the ring form, and laminate with the inner peripheral wall of inlet pipe (6), first solenoid valve (5) are located the inside of device storehouse (1).

3. A multi-channel micro-bioreactor according to claim 2, wherein: the bottom surface fixedly connected with solid fixed ring (10) of apron (9), the bottom surface of solid fixed ring (10) has seted up groove (12) of stepping down, device storehouse (1) extend to the inside of groove (12) of stepping down, the inside fixedly connected with fixed bolster (11) of groove (12) of stepping down.

4. A multi-channel micro-bioreactor according to claim 3, wherein: the fixing ring (10) and the device bin (1) are tightly attached to the fixing pad (11).

5. A multi-channel micro-bioreactor according to claim 3, wherein: the stirring assembly comprises a driving motor (8), the driving motor (8) is fixedly arranged on the top surface of a cover plate (9), one end of the output end of the driving motor (8) penetrates through the cover plate (9) and extends to a rotating rod (4) on the inner bottom wall of a reaction bin (2), a limiting hole (19) is formed in the inner bottom wall of the reaction bin (2), the rotating rod (4) extends to the inside of the limiting hole (19), stirring rods (13) with a plurality of stirring rods (13) are fixedly connected to the left side surface and the right side surface of the rotating rod (4), and connecting rods (17) are vertically fixed between every two adjacent stirring rods (13).

6. A multi-channel micro-bioreactor according to claim 5, wherein: the shape of reaction bin (2) is inside cavity and the cylinder that the top surface lacks, a plurality of puddler (13) all are located the inside of reaction bin (2), are located a plurality of puddler (13) of homonymy are linear equidistance from last down and distribute, bull stick (4) and spacing hole (19) clearance fit.

7. A multi-channel micro-bioreactor according to claim 5, wherein: the utility model provides a device, including device storehouse (1), device storehouse (1) are arranged in the device, water conservancy diversion subassembly includes water conservancy diversion storehouse (3), the inside in device storehouse (1) is connected in water conservancy diversion storehouse (3) and reaction storehouse (2) fixed connection, bull stick (4) run through water conservancy diversion storehouse (3), equal fixedly connected with supporting leg (15) all around the bottom surface in device storehouse (1), the interior bottom wall fixedly connected with quantity in reaction storehouse (2) is two and one end runs through reaction storehouse (2) and extends row material pipe (14) of device storehouse (1) bottom, two the equal fixed mounting of outer peripheral wall of row material pipe (14) has second solenoid valve (16).

8. A multi-channel micro-bioreactor according to claim 7, wherein: the shape of the diversion bin (3) is a cone with hollow inside and missing top surfaces and bottom surfaces, the two material discharging pipes (14) are symmetrically distributed left and right by taking the rotating rod (4) as the center, and the bottom ends of the two material discharging pipes (14) are inclined relatively.