PCR instrument with electric thermal cover assembly
Technical Field
The utility model belongs to the technical field of biological detection, and relates to a thermal cover assembly of a PCR instrument.
Background
The rapid change of the ambient temperature is accompanied in the PCR gene amplification process, and the evaporation phenomenon of the reaction reagent greatly influences the normal operation of the reaction and the result judgment. In order to overcome the evaporation phenomenon of the existing PCR instrument in PCR gene amplification, the thermal cover technology is widely applied. The existing hot cover pressing plate is mainly driven by a screw rod manually. In order to improve detection efficiency and reduce labor cost, a PCR instrument for electrically realizing lifting of a heat cover appears in the prior art, for example, chinese patent literature discloses an utility model patent of an automatic cover opening and closing mechanical device for PCR detection, the bulletin number is CN215668000U, a motor drives a threaded rod to drive a heat cover pressing plate to lift, the heat cover pressing plate is inclined easily due to the lifting structure, test tubes at different positions are stressed unevenly, and sealing of the test tubes is affected.
Disclosure of Invention
The utility model provides a PCR instrument with an electric thermal cover assembly aiming at the problems in the prior art, and aims to overcome the defect that the existing electric thermal cover assembly is easy to cause uneven stress of test tubes at different positions.
The utility model is realized in the following way:
the utility model provides a PCR appearance with electric heat lid subassembly, includes the organism and installs heat lid subassembly on the organism, heat lid subassembly is including fixing base on the organism, its characterized in that, set up on the base can be relative the mounting panel of base lateral movement and drive the first driving piece that the mounting panel removed, be equipped with on the mounting panel can be relative lifter plate and the drive that the mounting panel reciprocated the second driving piece that the lifter plate removed, be equipped with the heat lid clamp plate on the lifter plate in order to follow the lifter plate removes, the mounting panel with be equipped with guide assembly between the heat lid clamp plate.
When the detection is needed, the first driving piece drives the mounting plate to the working position, the machine body is provided with a detection disc for placing test tubes to be detected, when the mounting plate is in the working position, the hot cover pressing plate is located right above the detection disc, the second driving piece drives the lifting plate to descend and drives the hot cover pressing plate to descend until the hot cover pressing plate presses the test tubes, and the hot cover pressing plate heats so that the test tubes are in a proper temperature environment. The guide assembly can effectively guarantee the angle of the hot cover pressing plate relative to the mounting plate, for example, guarantee that the hot cover pressing plate and the mounting plate are parallel, incline when avoiding the lifter plate to drive the hot cover pressing plate to lift, guarantee that the hot cover pressing plate can evenly press on the test tube, the test tube compression degree of each position on the detection disc can not appear great gap, guarantee the whole gas tightness of test tube, reduce the evaporation phenomenon, the normal clear of the reaction of being convenient for improves the accuracy of result judgement.
Preferably, the guide assembly comprises a guide post fixed on the mounting plate or the hot cover pressing plate and a guide piece fixed on the hot cover pressing plate or the mounting plate, wherein the guide piece is provided with a guide groove matched with the guide post. The parallelism of the hot cover pressing plate and the mounting plate can be effectively guaranteed through the cooperation of the guide post and the guide groove. Two groups of guide components are respectively arranged at two ends of the hot cover pressing plate and the mounting plate.
Preferably, a guide post is fixed on the hot cover pressing plate, the guide post penetrates through the lifting plate, a limiting part is arranged at the upper end of the guide post, an elastic part is arranged between the hot cover pressing plate and the lifting plate, and the elastic force of the elastic part enables the hot cover pressing plate to move downwards relative to the lifting plate area. When the lifter plate descends, the lifter plate mainly pushes the hot cover pressing plate to move downwards through the elastic piece, after the hot cover pressing plate abuts against the test tube, the lifter plate continues to move downwards, the position of the hot cover pressing plate is unchanged, and the elastic piece deforms, so that the hot cover pressing plate has a certain floating space relative to the lifter plate, the test tube is prevented from being stressed too much, and the test tube can be guaranteed to be subjected to enough pressure to improve the sealing performance. The elastic piece is a spring and is sleeved on the guide post, and the four guide posts correspond to the four springs, so that the thrust of the lifting plate to the hot cover pressing plate is balanced. When the lifting plate ascends, the lifting plate moves the hot cover pressing plate upwards by pulling the guide post. The stop may be a nut or a nut.
Preferably, the lifting plate is provided with a pressure sensor, the hot cover pressing plate is provided with an abutting structure, and when the hot cover pressing plate is at the lower limit position, the abutting structure is in abutting connection with the pressure sensor. The pressure sensor is connected with a controller of the PCR instrument, pressure information is transmitted to the controller, and the controller judges the compression strength of the test tube according to the pressure condition, so that the second driving piece is controlled to stop driving the lifting plate to descend according to the preset pressure, and the descending amplitude of the lifting plate is controlled better by the PCR instrument.
Preferably, the hot cover pressing plate is provided with a connecting column, the connecting column is provided with a connecting groove with an upward opening, and the connecting groove is fixedly provided with an abutting column. The butt post can with pressure sensor butt, and the state of the hot lid clamp plate of pressure sensor detection of being convenient for. When the hot cover pressing plate does not act on the test tube, the abutting column can keep a gap with the pressure sensor, and when the hot cover pressing plate presses on the test tube and moves upwards relative to the lifting plate, the abutting column is in contact with the pressure sensor.
Preferably, an elastic pad is arranged on the lower surface of the hot cover pressing plate. The heat lid clamp plate presses on the test tube through the elastic cushion, and the elastic cushion can produce the deformation parcel and live the upper end of test tube for the leakproofness of test tube is better, improves the accuracy that detects.
Preferably, the second driving piece is vertically staggered with the lifting plate, a bedplate is fixed on the lifting plate, and one end of the bedplate extends out of the lifting plate and is connected with the second driving piece. The second driving piece drives the bedplate, and the bedplate drives the lifter plate, and the bedplate is the platy structure and can be connected with the lifter plate more stably, is favorable to lifter plate and mounting panel parallel to better assurance lifter plate and hot cover clamp plate are parallel. The second driving piece and the lifting plate are arranged in a staggered mode, so that the second driving piece is convenient to fix, and the overall height of the heat cover assembly is reduced. The second driving piece is a motor, and the motor is connected with the bedplate through a screw rod mechanism.
Preferably, a first position sensing assembly is provided between the mounting plate and the lifter plate to detect an upper limit position of the lifter plate. The first position sensing assembly comprises a photoelectric sensor and a trigger piece, the photoelectric sensor is connected with the controller, and when the first position sensing assembly detects that the lifting plate rises to the upper limit position, the controller controls the second driving piece to enable the lifting plate to stop rising.
Preferably, the first driving piece is connected with the mounting plate through a screw rod mechanism, a guide rail is fixed on the base, a guide block is fixed on the mounting plate, and a guide groove is formed in the guide block and matched with the guide rail. Thus, the first driving piece can stably drive the mounting plate to switch between the working position and the uncovering position.
Preferably, a second position sensing assembly is arranged between the base and the mounting plate to detect the working position of the mounting plate, and a third position sensing assembly is arranged between the base and the mounting plate to detect the cover opening position of the mounting plate. The second position sensing assembly and the third position sensing assembly both comprise photoelectric sensors and triggering pieces, and when the second position sensing assembly detects that the mounting plate reaches the working position, the controller controls the first driving piece to stop working and controls the second driving piece to drive the lifting plate to descend. When the third position sensing assembly detects that the mounting plate reaches the cover opening position, the controller controls the first driving piece to stop working, and at the moment, a worker can take and put the test tube.
The heat cover assembly is sleeved with a shell, a manual operation hole is formed in the shell, and an operation part is arranged on the second driving piece. When the second driving piece cannot electrically drive the lifting plate, an operator passes through the manual operation hole through a tool such as a wrench and acts on the operation part to enable the second driving piece to manually rotate so as to drive the lifting plate to lift.
According to the PCR instrument with the electric thermal cover assembly, the guide assembly can effectively ensure the angle of the thermal cover pressing plate relative to the mounting plate, the thermal cover pressing plate is prevented from inclining when being driven to lift by the lifting plate, the thermal cover pressing plate is ensured to be uniformly pressed on the test tube, the compression degree of the test tube at each position on the detection disc cannot be greatly different, the air tightness of the whole test tube is ensured, the evaporation phenomenon is reduced, the normal operation of the reaction is facilitated, and the accuracy of result judgment is improved.
Drawings
FIG. 1 is a schematic diagram of the structure of a PCR apparatus;
FIG. 2 is a schematic view of a first angle of a partial structure of a PCR instrument;
FIG. 3 is a second angular schematic view of the partial structure of the PCR instrument;
FIG. 4 is a first angular schematic view of the thermal cover assembly;
FIG. 5 is an enlarged view of portion A of FIG. 4;
FIG. 6 is a second angular schematic view of the thermal cover assembly;
FIG. 7 is a schematic cross-sectional structural view of the thermal cover assembly;
fig. 8 is an enlarged view of a portion B in fig. 7.
The drawings are marked with the following description: 100. a body; 200. a base; 210. a first driving member; 211. a guide rail; 212. a guide block; 300. a mounting plate; 310. a second driving member; 311. a platen; 400. a lifting plate; 410. a pressure sensor; 500. a thermal cover platen; 510. a guide post; 520. an elastic member; 530. a limiting piece; 540. a connecting column; 550. abutting the column; 560. an elastic pad; 610. a guide post; 620. a base; 630. a guide member; 640. a guide groove; 710. a first position sensing assembly; 720. a second position sensing assembly; 730. a third position sensing assembly; 740. a photoelectric sensor; 750. a trigger; 800. a housing; 810. a manual operation hole; 900. the disc is inspected.
Detailed Description
The following detailed description of the embodiments of the present utility model is provided with reference to the accompanying drawings, so that the technical scheme of the present utility model can be understood and mastered more easily. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.
The present embodiment provides a PCR apparatus, as shown in fig. 1-8, including a machine body 100 and a thermal cover assembly mounted on the machine body 100, wherein the thermal cover assembly includes a base 200 fixed on the machine body 100, a mounting plate 300 capable of moving transversely relative to the base 200 and a first driving member 210 for driving the mounting plate 300 to move are provided on the base 200, a lifting plate 400 capable of moving up and down relative to the mounting plate 300 and a second driving member 310 for driving the lifting plate 400 to move are provided on the mounting plate 300, a thermal cover pressing plate 500 is provided on the lifting plate 400 to move along with the lifting plate 400, and a guiding assembly is provided between the mounting plate 300 and the thermal cover pressing plate 500. When the test is needed, the first driving member 210 drives the mounting plate 300 to the working position, the machine body 100 is provided with the test tray 900 for placing test tubes to be tested, when the mounting plate 300 is in the working position, the hot cover pressing plate 500 is positioned right above the test tray 900, the second driving member 310 drives the lifting plate 400 to descend and drives the hot cover pressing plate 500 to descend until the hot cover pressing plate 500 presses the test tubes, and the hot cover pressing plate 500 heats up to enable the test tubes to be in a proper temperature environment.
As shown in fig. 4 and 5, the guide assembly includes guide posts 610 fixed to the mounting plate 300 and guide members 630 fixed to the hot cover platen 500, and the guide members 630 have guide grooves 640 and guide posts 610 engaged with each other. The mounting plate 300 is detachably fixed with an L-shaped seat 620, and the guide post 610 is fixed on the seat 620, thereby improving the stability of the guide post 610. In alternative embodiments, guide posts 610 may also be provided on the hot cover platen 500 with guides 630 secured to the mounting plate 300.
As shown in fig. 5 and 8, a guide post 510 is fixed on the hot cover pressing plate 500, the guide post 510 passes through the lifting plate 400, a limiting member 530 is disposed at the upper end of the guide post 510, an elastic member 520 is disposed between the hot cover pressing plate 500 and the lifting plate 400, and the elastic force of the elastic member 520 makes the hot cover pressing plate 500 move downward relative to the lifting plate 400. When lifter plate 400 descends, lifter plate 400 mainly pushes hot lid clamp plate 500 to move down through elastic component 520, and after hot lid clamp plate 500 supports to the test tube, lifter plate 400 continues to move down, and hot lid clamp plate 500 position is unchangeable, and elastic component 520 warp for hot lid clamp plate 500 has certain floating space for lifter plate 400 like this, avoids the test tube atress too big, can guarantee again that the test tube can receive sufficient pressure in order to improve the leakproofness. The elastic member 520 is a spring, and is sleeved on the guide post 510, and the guide post 510 has four springs corresponding to the four springs, which is beneficial to balancing the thrust of the lifting plate 400 to the hot cover pressing plate 500. When the lifter plate 400 is lifted, the lifter plate 400 moves up the hot cover platen 500 by pulling the guide post 510. The stop 530 may be a nut or a nut. The guide post 510 may be directly or indirectly secured to the hot cap platen 500.
As shown in fig. 8, the lifting plate 400 is provided with a pressure sensor 410, and the thermal cover pressing plate 500 is provided with an abutting structure, and when the thermal cover pressing plate 500 is at the lower limit position, the abutting structure abuts against the pressure sensor 410. The pressure sensor 410 is connected with a controller of the PCR instrument, and transmits pressure information to the controller, and the controller determines the compression strength of the test tube according to the pressure condition, so that the second driving member 310 is controlled to stop driving the lifting plate 400 to descend according to the preset pressure, and the PCR instrument can better control the descending amplitude of the lifting plate 400. The thermal cover pressing plate 500 is provided with a connecting column 540, the connecting column 540 is provided with a connecting groove with an upward opening, and an abutting column 550 is fixed on the connecting groove. The abutment post 550 can abut against the pressure sensor 410, facilitating the pressure sensor 410 to detect the state of the hot cover platen 500. When the hot cap platen 500 is not acting on the test tube, the abutment post 550 may be in clearance with the pressure sensor 410, and when the hot cap platen 500 is pressed against the test tube and moves up relative to the lift plate 400, the abutment post 550 contacts the pressure sensor 410.
As shown in fig. 6 and 8, the lower surface of the hot cover platen 500 is provided with an elastic pad 560. The heat cover pressing plate 500 is pressed on the test tube through the elastic pad 560, and the elastic pad 560 can deform to wrap the upper end of the test tube, so that the sealing performance of the test tube is better, and the detection accuracy is improved.
As shown in fig. 4, the second driving member 310 is vertically staggered with respect to the lifting plate 400, and the lifting plate 400 is fixed with a platen 311, and one end of the platen 311 extends out of the lifting plate 400 and is connected to the second driving member 310. The second driving piece 310 drives the platen 311, the platen 311 drives the lifting plate 400, and the platen 311 is in a plate-shaped structure and can be more stably connected with the lifting plate 400, so that the lifting plate 400 is parallel to the mounting plate 300, and the lifting plate 400 and the hot cover pressing plate 500 are better ensured to be parallel. The second driving member 310 is arranged in a staggered manner with the lifting plate 400, so that the second driving member 310 is conveniently fixed, and the overall height of the heat cover assembly is reduced. The second driving member 310 is a motor, and the motor is connected to the platen 311 through a screw mechanism.
As shown in fig. 4 and 5, a first position sensing assembly 710 is provided between the mounting plate 300 and the lifter plate 400 to detect the upper limit position of the lifter plate 400. The first position sensing assembly 710 includes a photo sensor 740 and a trigger 750, the photo sensor 740 being connected to a controller, and when the first position sensing assembly 710 detects that the lifter plate 400 is lifted to the upper limit position, the controller controls the second driving member 310 such that the lifter plate 400 stops lifting.
As shown in fig. 2 to 4, the first driving member 210 is connected to the mounting plate 300 through a screw mechanism, a guide rail 211 is fixed to the base 200, a guide block 212 is fixed to the mounting plate 300, and a guide groove is formed in the guide block 212 to be matched with the guide rail 211. The first driving member 210 is a motor such that the first driving member 210 can stably drive the mounting plate 300 to switch between the operating position and the door opening position.
As shown in fig. 6, a second position sensing assembly 720 is provided between the base 200 and the mounting plate 300 to detect the working position of the mounting plate 300, and a third position sensing assembly 730 is provided between the base 200 and the mounting plate 300 to detect the uncapping position of the mounting plate 300. The second position sensing assembly 720 and the third position sensing assembly 730 both include a photoelectric sensor 740 and a trigger 750, and when the second position sensing assembly 720 detects that the mounting plate 300 reaches the working position, the controller controls the first driving member 210 to stop working and controls the second driving member 310 to drive the lifting plate 400 to descend. When the third position sensing assembly 730 detects that the mounting plate 300 reaches the uncapping position, the controller controls the first driving member 210 to stop working, and the worker can take and put the test tube.
As shown in fig. 1, the heat cover assembly is externally provided with a housing 800, a manual operation hole 810 is provided in the housing 800, and an operation part is provided in the second driving member 310. When the operator cannot electrically drive the lifter plate 400 by the second driver 310, the operator passes through the manual operation hole 810 by a tool such as a wrench, and applies the tool to the operation portion so that the second driver 310 is manually rotated to drive the lifter plate 400 to be lifted.