CN220099051U - Stem cell exosome culture apparatus - Google Patents
Stem cell exosome culture apparatus Download PDFInfo
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- CN220099051U CN220099051U CN202321157911.6U CN202321157911U CN220099051U CN 220099051 U CN220099051 U CN 220099051U CN 202321157911 U CN202321157911 U CN 202321157911U CN 220099051 U CN220099051 U CN 220099051U
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- fixedly connected
- incubator body
- inner cavity
- incubator
- sensor
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 45
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 19
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 30
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 28
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 28
- 239000001301 oxygen Substances 0.000 claims abstract description 28
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 15
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 15
- 238000009434 installation Methods 0.000 claims abstract description 10
- 239000004973 liquid crystal related substance Substances 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 239000007921 spray Substances 0.000 claims description 8
- 239000000428 dust Substances 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a stem cell exosome culture device, which comprises an incubator body and an installation cavity, wherein the front surface of the incubator body is hinged with a sealing door through a hinge, the inner cavity of the incubator body is provided with a placing plate, the upper end of the left side of the inner cavity of the incubator body is fixedly connected with a temperature sensor, the lower end of the left side of the inner cavity of the incubator body is fixedly connected with a humidity sensor, and the upper end of the right side of the inner cavity of the incubator body is fixedly connected with an oxygen sensor; according to the utility model, the temperature sensor, the humidity sensor, the oxygen sensor, the carbon dioxide sensor, the PLC and the liquid crystal display are arranged, so that the temperature and the humidity of the inner cavity of the incubator body and the oxygen content can be conveniently monitored, a worker can conveniently know the temperature and the humidity of the culture environment of the exosome, the temperature of the culture environment can be conveniently regulated and controlled, the culture efficiency of the exosome is effectively improved, and convenience is brought to the culture work of the exosome.
Description
Technical Field
The utility model belongs to the field of exosome culture devices, and particularly relates to a stem cell exosome culture device.
Background
The stem cell exosomes are small vesicles produced by stem cells during division and propagation, are rich in cholesterol, sphingomyelin and the like, mainly can regulate proliferation, differentiation and the like of cells, can play a role in coordinating the inside of a human body, can secrete a plurality of cells and body fluids in the human body, and can be placed in an incubator for cultivation when the cell exosomes are optimally cultivated;
according to the Chinese patent application number: 202221534254.8 the utility model discloses a novel adipose-derived stem cell culture device, which comprises a device main body, the inside of device main part is equipped with multilayer baffle, multilayer baffle is with a plurality of culture bins of device main part internal partition department, and every culture bin's inside all slidable mounting has pull box, the upper surface of pull box is equipped with multiunit fixture, fixture includes movable rod, dead lever, pull block and rotary disk, the inside of pull box is hollow setting, and the upper surface corresponds dead lever, movable rod position department level has seted up the spout, spout fixed connection is passed to the bottom of dead lever, the movable rod bottom pass the spout and with spout sliding connection, the bottom lateral wall fixedly connected with arm-tie of movable rod, the arm-tie level sets up and keeps away from the one end fixedly connected with first spring of slide lever, compares case adipose-derived stem cell exosome culture device, and is convenient to culture vessel's fixed mode operation;
the effectual solution of comparative case is harder to realize to single operation when fixing culture apparatus, uses inconvenient problem to the fixed mode of household utensils, has the advantage of simple operation when fixed to the household utensils, however in the in-service use, owing to lack the monitoring to humiture and oxygen content in the incubator for the staff can not in time master the culture environment of exosome, thereby lead to the humiture and the oxygen content in the inconvenient control incubator, and then influenced the cultivation of exosome, brought inconvenience for the cultivation work of exosome.
In summary, the present utility model provides a stem cell exosome culture apparatus to solve the above-mentioned problems.
Disclosure of Invention
In order to solve the technical problems, the utility model provides a stem cell exosome culture device, which aims to solve the problems that in the prior art, due to lack of monitoring of temperature and humidity and oxygen content in an incubator, a worker cannot timely master the culture environment of exosome, so that the temperature and humidity and oxygen content in the incubator are inconvenient to control, the culture of exosome is further influenced, and inconvenience is brought to the culture work of exosome.
The utility model provides a stem cell exosome culture apparatus, includes incubator body and installation cavity, the front of incubator body is articulated through the hinge to have the sealing door, the inner chamber of incubator body is provided with places the board, the positive upper end fixedly connected with temperature sensor of incubator body inner chamber left side, the positive lower extreme fixedly connected with humidity transducer of incubator body inner chamber left side, the upper end fixedly connected with oxygen sensor on incubator body inner chamber right side, the lower extreme fixedly connected with carbon dioxide sensor on incubator body inner chamber right side, the left side fixedly connected with refrigerator body of installation cavity inner chamber bottom, the right side fixedly connected with electromagnetic heater of installation cavity inner chamber bottom, the right side intercommunication of incubator body has the intake pipe, the positive right side fixedly connected with PLC controller of incubator body, the positive left side fixedly connected with liquid crystal display of incubator body.
Preferably, the surface of the air inlet pipe is communicated with an air inlet electromagnetic valve, and the top of the inner cavity of the installation cavity is fixedly connected with a dust screen.
Preferably, the top fixedly connected with humidifier body of incubator body, the top fixedly connected with atomising head of incubator body inner chamber, the top intercommunication of atomising head has communicating pipe, the one end of keeping away from the atomising head of communicating pipe runs through to the outside of incubator body and communicates with the humidifier body.
Preferably, the input ends of the refrigerator body, the electromagnetic heater, the air inlet electromagnetic valve and the humidifier body are electrically connected with the output end of the PLC, and the input end of the PLC is electrically connected with the output ends of the temperature sensor, the humidity sensor, the oxygen sensor and the carbon dioxide sensor respectively.
Preferably, a servo motor is fixedly connected to the center of the bottom of the inner cavity of the installation cavity, and a fan blade is fixedly connected to an output shaft of the servo motor.
Preferably, both sides of incubator body inner chamber are all fixedly connected with fixed plate, place the board and be located between two fixed plates, two the spout has all been seted up to one side that the fixed plate is relative, the inner chamber sliding connection of spout has the slider, the one end that the spout inner chamber was kept away from to the slider is with place board fixed connection.
Preferably, the front of incubator body has seted up the observation window, the front fixedly connected with handle of incubator body.
Compared with the prior art, the utility model has the following beneficial effects:
1. according to the utility model, the temperature sensor, the humidity sensor, the oxygen sensor, the carbon dioxide sensor, the PLC and the liquid crystal display are arranged, so that the temperature and the humidity of the inner cavity of the incubator body and the oxygen content can be conveniently monitored, the temperature sensor can monitor the culture temperature, the humidity sensor can monitor the humidity of the inner cavity of the incubator body, the oxygen sensor can monitor the oxygen content of the inner cavity of the incubator body, the carbon dioxide sensor can monitor the carbon dioxide content of the inner cavity of the incubator body, the temperature sensor, the humidity sensor, the oxygen sensor and the carbon dioxide sensor send monitoring results to the PLC, the PLC sends the results to the liquid crystal display and displays the results through the liquid crystal display, thereby being convenient for staff to know the temperature and the humidity of the culture environment of the exosome, being convenient to regulate and control the culture environment temperature, effectively improving the culture efficiency of the exosome and bringing convenience to the culture work of the exosome.
2. According to the utility model, the incubator body, the mounting cavity, the sealing door, the placing plate, the refrigerator body, the electromagnetic heater and the air inlet pipe are arranged, so that the effect of cultivating the exosomes is achieved, the refrigerator body and the electromagnetic heater can adjust the cultivation temperature of the inner cavity of the incubator body, the air inlet pipe is communicated with the peripheral oxygen tank, and oxygen can be conveyed to the inner cavity of the incubator body, thereby meeting the environment of exosome cultivation, effectively improving the cultivation efficiency of the exosomes and bringing convenience to the cultivation work of the exosomes.
Drawings
FIG. 1 is a schematic diagram of the front view of the present utility model;
FIG. 2 is a schematic cross-sectional elevation view of the incubator body of the present utility model;
FIG. 3 is a schematic view of a partial enlarged structure of the present utility model at A in FIG. 1;
fig. 4 is a system flow diagram of the present utility model.
In the figure:
1. an incubator body; 2. a mounting chamber; 3. sealing the door; 4. placing a plate; 5. a temperature sensor; 6. a humidity sensor; 7. an oxygen sensor; 8. a carbon dioxide sensor; 9. a refrigerator body; 10. an electromagnetic heater; 11. an air inlet pipe; 12. a PLC controller; 13. a liquid crystal display; 14. an air inlet electromagnetic valve; 15. a humidifier body; 16. a spray head; 17. a communicating pipe; 18. a servo motor; 19. a fan blade; 20. a fixing plate; 21. a chute; 22. a slide block; 23. an observation window; 24. a handle.
Detailed Description
Embodiments of the present utility model are described in further detail below with reference to the accompanying drawings and examples. The following examples are illustrative of the present utility model and are not intended to limit the scope of the utility model.
As shown in fig. 1-4, the utility model provides a stem cell exosome culture device, which comprises a culture box body 1 and a mounting cavity 2, wherein the front surface of the culture box body 1 is hinged with a sealing door 3 through a hinge, the inner cavity of the culture box body 1 is provided with a placing plate 4, the upper end of the left side of the inner cavity of the culture box body 1 is fixedly connected with a temperature sensor 5, the lower end of the left side of the inner cavity of the culture box body 1 is fixedly connected with a humidity sensor 6, the upper end of the right side of the inner cavity of the culture box body 1 is fixedly connected with an oxygen sensor 7, the lower end of the right side of the inner cavity of the culture box body 1 is fixedly connected with a carbon dioxide sensor 8, the left side of the bottom of the inner cavity of the mounting cavity 2 is fixedly connected with a refrigerator body 9, the right side of the bottom of the inner cavity of the mounting cavity 2 is fixedly connected with an electromagnetic heater 10, the right side of the culture box body 1 is communicated with an air inlet pipe 11, the right side of the front surface of the culture box body 1 is fixedly connected with a PLC controller 12, and the left side of the front surface of the culture box body 1 is fixedly connected with a liquid crystal display 13.
As one implementation mode of the utility model, the surface of the air inlet pipe 11 is communicated with the air inlet electromagnetic valve 14, the top of the inner cavity of the installation cavity 2 is fixedly connected with the dust screen, and the air inlet electromagnetic valve 14 is arranged, so that the effect of conveniently controlling the air inlet flow is achieved.
As one embodiment of the present utility model, the humidifier body 15 is fixedly connected to the top of the incubator body 1, the spray head 16 is fixedly connected to the top of the inner cavity of the incubator body 1, the communication pipe 17 is connected to the top of the spray head 16, one end of the communication pipe 17, which is far away from the spray head 16, penetrates through the outside of the incubator body 1 and is communicated with the humidifier body 15, and by providing the humidifier body 15, the spray head 16 and the communication pipe 17, the effect of humidifying the inner cavity of the incubator body 1 is achieved, so that the culture environment of the exosome can be improved.
As an embodiment of the present utility model, the input ends of the refrigerator body 9, the electromagnetic heater 10, the air intake electromagnetic valve 14 and the humidifier body 15 are electrically connected to the output ends of the PLC controller 12, and the input ends of the PLC controller 12 are electrically connected to the output ends of the temperature sensor 5, the humidity sensor 6, the oxygen sensor 7 and the carbon dioxide sensor 8, respectively.
As one implementation mode of the utility model, the servo motor 18 is fixedly connected to the center of the bottom of the inner cavity of the mounting cavity 2, the fan blade 19 is fixedly connected to the output shaft of the servo motor 18, and the effect of accelerating the airflow in the inner cavity of the incubator body 1 is achieved by arranging the servo motor 18 and the fan blade 19, the fan blade 19 is driven to rotate by the rotation of the output shaft of the servo motor 18, and the airflow in the inner cavity of the mounting cavity 2 is driven to flow to the inner cavity of the incubator body 1 by the airflow generated by the rotation of the fan blade 19, so that the effect of accelerating the airflow in the inner cavity of the incubator body 1 can be achieved.
As one implementation mode of the utility model, the two sides of the inner cavity of the incubator body 1 are fixedly connected with the fixed plates 20, the placing plate 4 is positioned between the two fixed plates 20, the opposite sides of the two fixed plates 20 are provided with the sliding grooves 21, the inner cavity of the sliding grooves 21 is slidably connected with the sliding blocks 22, one end of the sliding blocks 22 far away from the inner cavity of the sliding grooves 21 is fixedly connected with the placing plate 4, the effect of stably supporting the placing plate 4 is achieved by arranging the fixed plates 20, the effect of conveniently extracting the placing plate 4 from between the two fixed plates 20 is achieved by arranging the sliding grooves 21 and the sliding blocks 22, and therefore, the culture dish can be placed or taken conveniently.
As one embodiment of the utility model, the front surface of the incubator body 1 is provided with the observation window 23, the front surface of the incubator body 1 is fixedly connected with the handle 24, the observation window 23 is arranged to facilitate observation of the culture condition of the inner cavity of the incubator body 1, and the handle 24 is arranged to facilitate opening and closing of the sealing door 3.
The specific working principle is as follows:
firstly, placing a culture dish with exosomes on the top of a placing plate 4, then closing a sealing door 3, setting culture temperature, humidity and oxygen content values through a PLC (programmable logic controller) 12, monitoring the temperature of the inner cavity of the incubator body 1 by a temperature sensor 5, monitoring the humidity of the inner cavity of the incubator body 1 by a humidity sensor 6, monitoring the oxygen content of the inner cavity of the incubator body 1 by an oxygen sensor 7, monitoring the carbon dioxide content of the inner cavity of the incubator body 1 by a carbon dioxide sensor 8, sending monitoring results to the PLC 12 by the temperature sensor 5, the humidity sensor 6, the oxygen sensor 7 and the carbon dioxide sensor 8, sending the results to a liquid crystal display 13 by the PLC 12, displaying by the liquid crystal display 13, when the values exceed or are lower than the preset values, the PLC 12 respectively starts the refrigerator body 9, the electromagnetic heater 10, the humidifier body 15 and the air inlet electromagnetic valve 14, the refrigerator body 9 and the electromagnetic heater 10 can improve the culture temperature of the inner cavity of the incubator body 1, the humidifier body 15 can humidify the inner cavity of the incubator body 1, the air inlet electromagnetic valve 14 can convey oxygen to the inner cavity of the incubator body 1, thereby improving the culture environment of the exosome, facilitating the staff to know the temperature and humidity condition of the culture environment of the exosome, further being convenient to regulate and control the culture environment temperature, effectively improving the culture efficiency of the exosome, bringing convenience to the culture work of the exosome, so as to solve the problems that the staff cannot timely master the culture environment of the exosome due to the lack of monitoring of the temperature and the oxygen content in the incubator, thereby causing inconvenient control of the temperature and the oxygen content of the exosome in the incubator, thereby influencing the culture of exosomes and bringing inconvenience to the culture work of exosomes.
The embodiments of the present utility model have been shown and described for the purpose of illustration and description, it being understood that the above embodiments are illustrative and not to be construed as limiting the utility model, and that variations, modifications, alternatives and variations may be made therein by one of ordinary skill in the art without departing from the scope of the utility model.
Claims (7)
1. The utility model provides a stem cell exosome culture apparatus, includes incubator body (1) and installation cavity (2), its characterized in that: the incubator comprises an incubator body (1), a sealing door (3) is hinged to the front face of the incubator body (1) through a hinge, a placing plate (4) is arranged in an inner cavity of the incubator body (1), a temperature sensor (5) is fixedly connected to the upper end of the left side of the inner cavity of the incubator body (1), a humidity sensor (6) is fixedly connected to the lower end of the left side of the inner cavity of the incubator body (1), an oxygen sensor (7) is fixedly connected to the upper end of the right side of the inner cavity of the incubator body (1), a carbon dioxide sensor (8) is fixedly connected to the lower end of the right side of the inner cavity of the incubator body (1), a refrigerator body (9) is fixedly connected to the left side of the bottom of the inner cavity of the mounting chamber (2), an electromagnetic heater (10) is fixedly connected to the right side of the bottom of the inner cavity of the mounting chamber (2), an air inlet pipe (11) is communicated to the right side of the incubator body (1), a PLC (12) is fixedly connected to the right side of the inner cavity of the incubator body (1), and a liquid crystal display (13) is fixedly connected to the right side of the incubator body (1).
2. The stem cell exosome culture apparatus according to claim 1, wherein: the surface of the air inlet pipe (11) is communicated with an air inlet electromagnetic valve (14), and the top of the inner cavity of the installation cavity (2) is fixedly connected with a dust screen.
3. The stem cell exosome culture apparatus according to claim 2, wherein: the incubator is characterized in that the humidifier body (15) is fixedly connected to the top of the incubator body (1), the spray head (16) is fixedly connected to the top of the inner cavity of the incubator body (1), the communication pipe (17) is communicated with the top of the spray head (16), and one end, far away from the spray head (16), of the communication pipe (17) penetrates through the outside of the incubator body (1) and is communicated with the humidifier body (15).
4. The stem cell exosome culture apparatus according to claim 3, wherein: the refrigerator is characterized in that the input ends of the refrigerator body (9), the electromagnetic heater (10), the air inlet electromagnetic valve (14) and the humidifier body (15) are electrically connected with the output end of the PLC (12), and the input end of the PLC (12) is electrically connected with the output ends of the temperature sensor (5), the humidity sensor (6), the oxygen sensor (7) and the carbon dioxide sensor (8) respectively.
5. The stem cell exosome culture apparatus according to claim 1, wherein: the center of the bottom of the inner cavity of the installation cavity (2) is fixedly connected with a servo motor (18), and an output shaft of the servo motor (18) is fixedly connected with a fan blade (19).
6. The stem cell exosome culture apparatus according to claim 1, wherein: both sides of incubator body (1) inner chamber are all fixedly connected with fixed plate (20), place board (4) and be located between two fixed plates (20), two spout (21) have all been seted up to one side that fixed plate (20) are relative, the inner chamber sliding connection of spout (21) has slider (22), the one end that spout (21) inner chamber was kept away from to slider (22) is with place board (4) fixed connection.
7. The stem cell exosome culture apparatus according to claim 1, wherein: the front of incubator body (1) has seted up observation window (23), the front fixedly connected with handle (24) of incubator body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321157911.6U CN220099051U (en) | 2023-05-12 | 2023-05-12 | Stem cell exosome culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321157911.6U CN220099051U (en) | 2023-05-12 | 2023-05-12 | Stem cell exosome culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220099051U true CN220099051U (en) | 2023-11-28 |
Family
ID=88868379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321157911.6U Active CN220099051U (en) | 2023-05-12 | 2023-05-12 | Stem cell exosome culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220099051U (en) |
-
2023
- 2023-05-12 CN CN202321157911.6U patent/CN220099051U/en active Active
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