CN219930128U - Stem cell culture perfusion device - Google Patents
Stem cell culture perfusion device Download PDFInfo
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- CN219930128U CN219930128U CN202320556091.1U CN202320556091U CN219930128U CN 219930128 U CN219930128 U CN 219930128U CN 202320556091 U CN202320556091 U CN 202320556091U CN 219930128 U CN219930128 U CN 219930128U
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- gear
- rod
- cell culture
- stem cell
- inner tube
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 34
- 238000004113 cell culture Methods 0.000 title claims abstract description 22
- 230000010412 perfusion Effects 0.000 title claims abstract description 22
- 238000003756 stirring Methods 0.000 claims abstract description 39
- 239000007788 liquid Substances 0.000 claims description 41
- 244000309464 bull Species 0.000 claims description 11
- 238000007599 discharging Methods 0.000 claims description 7
- 230000002457 bidirectional effect Effects 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 15
- 238000000034 method Methods 0.000 description 6
- 230000001681 protective effect Effects 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000002894 multi-fate stem cell Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of stem cell culture perfusion, and discloses a stem cell culture perfusion device, which solves the problem that the existing stem cell culture perfusion is single in mixing and stirring direction and is not enough to quickly improve the activity of stem cells. According to the utility model, when stem cells are required to be stirred through the disc-type gear set, the side gears, the stirring rod I and the stirring rod II, the motor drives the driving rod to rotate, the driving rod drives the stirring rod to rotate, the stirring rod I drives the upper disc-type gear to be meshed with the side gears to rotate, the side gears further drive the lower disc-type gear to rotate, the lower disc-type gear and the upper disc-type gear reversely rotate, the side gears drive the rotating rod to rotate, and the rotating rod is fixedly connected with the connecting plate through the bearing, so that the lower disc-type gear drives the stirring rod II to rotate, the activity of the stem cells is improved more rapidly, and the stirring and mixing effects are better.
Description
Technical Field
The utility model relates to the technical field of stem cell culture perfusion, in particular to a stem cell culture perfusion device.
Background
Stem cells are multipotent cells with self-replication capacity, and can be differentiated into a plurality of functional cells under certain conditions, and after the cells and a culture medium are added into a reactor together, part of the conditioned medium is continuously taken out in the process of cell growth and product formation, and new culture medium is continuously poured in;
according to the search, the prior Chinese patent CN215050386U discloses a stem cell culture perfusion device, which comprises a shell, an inner container, a liquid inlet pipe and a liquid outlet pipe, wherein the inner container is arranged in the inner cavity of the shell, one side of the top surface of the shell is connected with the liquid inlet pipe in an embedded manner, the bottom surface of the shell is connected with the liquid outlet pipe in an embedded manner, a protective frame is arranged in the middle of the top surface of the shell, a servo motor is arranged in the inner cavity of the protective frame, a stirring paddle is arranged in the inner cavity, an electric heating plate is arranged on the outer wall surface of the inner cavity, heating liquid is filled between the shell and the inner container, a protective box is welded in the middle of the right side wall of the shell, a time-controlled switch is arranged at the top of the surface of the protective box, and a temperature regulating button is arranged at the bottom of the surface of the protective box, and the bottom end of the liquid inlet pipe is connected with the inner container in a conducting manner.
Although the activity of stem cells is improved by the stirring rod and the heating mechanism, the problems still exist in the operation and use process: foretell puddler can only rotate a direction to unidirectional puddler mixing time is longer, increases the electric quantity loss, increase cost, and puddler unidirectional rotation effect is not good.
Disclosure of Invention
The utility model aims to provide a stem cell culture perfusion device which is used for working, so that the problem that the activity of stem cells is not sufficiently and rapidly improved due to the fact that the existing stem cell culture perfusion is single in mixing and stirring direction is solved.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the stem cell culture perfusion device comprises a shell, wherein a liquid inlet for feeding is formed in the top of the shell, a liquid outlet for discharging is formed in the bottom of the shell, a liquid discharging control structure for controlling the liquid discharging is formed in the bottom of the liquid outlet, a motor for driving is arranged at the left end of the liquid inlet, a driving rod for driving is arranged at the bottom of the motor, a stirring rod I for mixing is arranged at the bottom of the driving rod, and a rotating structure for bidirectional stirring driving is arranged at the outer end of the middle of the stirring rod I;
the rotary structure comprises an upper disc-shaped gear arranged outside the stirring rod I, a lateral gear is arranged below the upper disc-shaped gear, a lower disc-shaped gear is arranged below the lateral gear, and a stirring rod II is arranged below the lower disc-shaped gear.
Further, the upper end of the upper disc type gear is provided with a connecting plate, the connecting plate is connected with the stirring rod through a bearing, the upper disc type gear is meshed with the lateral gear, the lateral gear is meshed with the lower disc type gear, and the upper disc type gear and the lower disc type gear rotate in opposite directions.
Further, the inside of side direction gear is provided with the bull stick, and the outside of bull stick is provided with the connecting plate to the connecting plate passes through the bearing and is connected with side direction gear, the inside of bull stick is provided with puddler two, and puddler two and puddler one opposite direction motion, and puddler two passes through the bearing with puddler one and be connected.
Further, the control liquid outlet structure comprises a fixed plate arranged at the bottom of the liquid outlet, a rotating rod is arranged at the bottom of the fixed plate, and the rotating rod is connected with the fixed plate through a bearing.
Further, the inside thread groove that is in bottom of rotary rod, and the inside gear that is provided with in bottom of rotary rod, the bottom of gear and rotary rod is threaded connection, and the screw rod has self-locking function with the bottom thread groove of rotary rod to the bottom of gear sets up the handle.
Further, the right-hand member of gear is provided with the tooth piece, and the right-hand member of tooth piece is provided with the inner tube to the tooth piece distributes in the surface of inner tube, the tooth piece is provided with 6, and the tooth piece is located the half circle of inner tube.
Further, the bottom of the inner cylinder is provided with big holes, the big holes are provided with three groups, and the three groups of big holes are positioned in the general of the inner cylinder, the other half of the bottom of the inner cylinder is a baffle, the outer part of the inner cylinder is provided with an outer cylinder, and the size of the outer cylinder is larger than that of the inner cylinder.
Further, the bottom of urceolus is provided with the macropore, and the macropore is the same position with the macropore of inner tube, urceolus bottom half is provided with three groups of aperture, and the position and the macropore of aperture are symmetric distribution, the surface of urceolus is provided with the channel, and the tooth piece is located the inside slip of channel.
Compared with the prior art, the utility model has the following beneficial effects:
the stem cell culture perfusion device provided by the utility model has the advantages that the existing stem cell culture perfusion mixing and stirring direction is single, so that the activity of stem cells is not sufficiently and rapidly improved; according to the utility model, when stem cells are required to be stirred, the driving rod is driven to rotate by the motor, the driving rod is driven to rotate by the driving rod, the stirring rod is driven to rotate by the first driving rod, the upper disc type gear is meshed with the lateral gear to rotate, the lower disc type gear is driven to rotate by the further lateral gear, the lower disc type gear and the upper disc type gear are reversely driven, the lateral gear drives the rotating rod to rotate, and the rotating rod is fixedly connected with the connecting plate through the bearing, so that the lower disc type gear drives the stirring rod to rotate, the activity of the stem cells is improved more rapidly, and the stirring and mixing effects are better.
Drawings
FIG. 1 is a schematic view of the overall front cross-section of the present utility model;
FIG. 2 is a schematic three-dimensional view of a gear set and a driving rod according to the present utility model;
FIG. 3 is a schematic three-dimensional structure of a liquid outlet control structure according to the present utility model;
FIG. 4 is a schematic diagram of an enlarged three-dimensional structure of a liquid outlet control structure according to the present utility model;
FIG. 5 is a three-dimensional schematic view of an inner barrel of the present utility model;
FIG. 6 is a schematic view of a three-dimensional structure of an outer cylinder according to the present utility model;
fig. 7 is a schematic top view of the outer cylinder and the inner cylinder of the present utility model.
In the figure: 1. a housing; 2. a liquid inlet; 3. a liquid outlet; 4. a motor; 5. a driving rod; 6. a stirring rod I; 7. a rotating structure; 71. a connecting plate; 72. an upper disc type gear; 73. a lateral gear; 74. a lower disc type gear; 75. a rotating rod; 76. a stirring rod II; 8. controlling a liquid outlet structure; 81. a fixing plate; 82. a rotating rod; 83. a gear; 84. a screw; 85. a handle; 86. tooth blocks; 87. an inner cylinder; 88. a channel; 89. an outer cylinder; 810. a large hole; 811. and (3) small holes.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
For a further understanding of the present utility model, the present utility model will be described in detail with reference to the drawings.
Referring to fig. 1-7, a stem cell culture perfusion device comprises a casing 1, wherein a liquid inlet 2 for feeding is formed in the top of the casing 1, a liquid outlet 3 for discharging is formed in the bottom of the casing 1, a liquid outlet control structure 8 for controlling discharging is formed in the bottom of the liquid outlet 3, a motor 4 for driving is arranged at the left end of the liquid inlet 2, a driving rod 5 for driving is arranged at the bottom of the motor 4, a stirring rod I6 for mixing is arranged at the bottom of the driving rod 5, and a rotating structure 7 for bidirectional stirring driving is arranged at the outer end of the middle part of the stirring rod I6;
the rotating structure 7 comprises an upper disc-shaped gear 72 arranged outside the stirring rod I6, a lateral gear 73 is arranged below the upper disc-shaped gear 72, a lower disc-shaped gear 74 is arranged below the lateral gear 73, and a stirring rod II 76 is arranged below the lower disc-shaped gear 74.
The utility model is further described below with reference to examples.
Example 1:
referring to fig. 1-2, the rotating structure 7 includes an upper disc gear 72 disposed outside the stirring rod one 6, a lateral gear 73 disposed below the upper disc gear 72, a lower disc gear 74 disposed below the lateral gear 73, a stirring rod two 76 disposed below the lower disc gear 74, a connecting plate 71 disposed at an upper end of the upper disc gear 72, and the connecting plate 71 connected to the stirring rod one 6 through a bearing, wherein the upper disc gear 72 is meshed with the lateral gear 73, the lateral gear 73 is meshed with the lower disc gear 74, and the upper disc gear 72 and the lower disc gear 74 rotate in opposite directions to facilitate driving of the stirring rod one 6 and the stirring rod two 76.
The inside of side gear 73 is provided with bull stick 75, and the outside of bull stick 75 is provided with connecting plate 71 to connecting plate 71 passes through the bearing and is connected with side gear 73, and the inside of bull stick 75 is provided with puddler two 76, and puddler two 76 and puddler one 6 opposite direction motion, and puddler two 76 and puddler one 6 pass through the bearing and are connected, and the mixing effect is better, improves the activity of stem cell.
Specifically, at first, staff pours stem cells that need cultivate into casing 1 inside through inlet 2, then the staff starts motor 4 through outside button, make motor 4 drive actuating lever 5 rotate, and actuating lever 5 drive puddler one 6 rotates, puddler one 6 drives upper disc type gear 72 and side gear 73 meshing rotation, further side gear 73 drives lower disc type gear 74 rotation, and lower disc type gear 74 and upper disc type gear 72 are reverse initiative, side gear 73 drives bull stick 75 rotation simultaneously, and bull stick 75 is fixed through the bearing and connecting plate 71 connection, make lower disc type gear 74 drive puddler two 76 rotations, thereby the activity of stem cells is improved more rapidly, the effect of stirring mixes is better.
Example 2:
referring to fig. 3-7, the control liquid outlet structure 8 includes a fixing plate 81 disposed at the bottom of the liquid outlet 3, a rotating rod 82 is disposed at the bottom of the fixing plate 81, the rotating rod 82 is connected with the fixing plate 81 through a bearing, a thread groove is disposed inside the bottom end of the rotating rod 82, a gear 83 is disposed inside the bottom end of the rotating rod 82, the gear 83 is in threaded connection with the bottom of the rotating rod 82, the screw 84 and the thread groove at the bottom of the rotating rod 82 have a self-locking function, and a handle 85 is disposed at the bottom of the gear 83, so that the inner cylinder 87 and the inner cylinder are conveniently aligned
The right-hand member of gear 83 is provided with tooth piece 86, and the right-hand member of tooth piece 86 is provided with inner tube 87, and tooth piece 86 distributes in the surface of inner tube 87, tooth piece 86 is provided with 6, and tooth piece 86 is located the half circle of inner tube 87, the bottom of inner tube 87 is provided with macropore 810, and macropore 810 is provided with three groups, and three groups macropore 810 are located the general of inner tube 87, the other half of inner tube 87 bottom is the baffle, the outside of inner tube 87 is provided with urceolus 89, and the size of urceolus 89 is greater than the size of inner tube 87, the bottom of urceolus 89 is provided with macropore 810, and macropore 810 and the macropore 810 of inner tube 87 are the same position, the other half of urceolus 89 bottom is provided with three groups of aperture 811, and the position of aperture 811 is the symmetric distribution with macropore 810, the surface of urceolus 89 is provided with channel 88, and tooth piece 86 is located the inside slip of channel 88.
Specifically, when the liquid outlet amount of the liquid outlet 3 is required to be regulated according to the capacities of different culture dishes, the screw rod 84 is driven to rotate by the rotating handle 85, the screw rod 84 is in threaded connection with the threaded groove at the bottom of the rotating rod 82 to rotate, and the top of the rotating rod 82 is connected with the fixed plate 81 through the bearing, the rotating rod 82 drives the gear 83 to rotate in meshed mode with the tooth block 86, as the tooth block 86 is located on the surface of the inner cylinder 87, the bottom of the inner cylinder 87 is provided with a half solid structure, the other half is provided with a large hole 810, again because the inner cylinder 87 is arranged in the outer cylinder 89, the surface of the outer cylinder 86 is provided with a channel 88, the tooth block 86 moves in the channel 88, and the half of the bottom of the outer cylinder 89 is uniformly provided with the large hole 810, and the other half is uniformly provided with small holes 811, and when the gear 83 drives the inner cylinder 87 connected with the tooth block 86 to rotate, the large hole 810 of the inner cylinder 87 corresponds to the large hole 810 of the outer cylinder 89, thereby the effect of dry cell liquid is completed, and when the 810 of the inner cylinder 87 corresponds to the small hole 811 of the outer cylinder 89, the effect of the dry cell liquid is also convenient to regulate the liquid outlet amount of the liquid outlet 3, and the large amount of the large hole is high in applicability.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The stem cell culture perfusion device comprises a shell (1), and is characterized in that: the top of the shell (1) is provided with a liquid inlet (2) for feeding, the bottom of the shell (1) is provided with a liquid outlet (3) for discharging liquid, the bottom of the liquid outlet (3) is provided with a liquid outlet control structure (8) for controlling liquid discharging, the left end of the liquid inlet (2) is provided with a motor (4) for driving, the bottom of the motor (4) is provided with a driving rod (5) for driving, the bottom of the driving rod (5) is provided with a stirring rod I (6) for mixing, and the outer end of the middle part of the stirring rod I (6) is provided with a rotating structure (7) for bidirectional stirring driving;
the rotary structure (7) comprises an upper disc-shaped gear (72) arranged outside the stirring rod I (6), a lateral gear (73) is arranged below the upper disc-shaped gear (72), a lower disc-shaped gear (74) is arranged below the lateral gear (73), and a stirring rod II (76) is arranged below the lower disc-shaped gear (74).
2. The stem cell culture perfusion device of claim 1, wherein: the upper end of the upper disc-shaped gear (72) is provided with a connecting plate (71), the connecting plate (71) is connected with the stirring rod I (6) through a bearing, the upper disc-shaped gear (72) is meshed with the lateral gear (73), the lateral gear (73) is meshed with the lower disc-shaped gear (74), and the upper disc-shaped gear (72) and the lower disc-shaped gear (74) rotate in opposite directions.
3. A stem cell culture perfusion device according to claim 2, wherein: the inside of side direction gear (73) is provided with bull stick (75), and the outside of bull stick (75) is provided with connecting plate (71) to connecting plate (71) are connected with side direction gear (73) through the bearing, the inside of bull stick (75) is provided with puddler two (76), and puddler two (76) and puddler one (6) opposite direction motion, and puddler two (76) are connected through the bearing with puddler one (6).
4. The stem cell culture perfusion device of claim 1, wherein: the control liquid outlet structure (8) comprises a fixed plate (81) arranged at the bottom of the liquid outlet (3), a rotating rod (82) is arranged at the bottom of the fixed plate (81), and the rotating rod (82) is connected with the fixed plate (81) through a bearing.
5. The stem cell culture perfusion device of claim 4, wherein: the inside thread groove that is in bottom of rotary rod (82), and the inside gear (83) that is provided with in bottom of rotary rod (82), the bottom of gear (83) and rotary rod (82) is threaded connection, and screw rod (84) have self-locking function with the bottom thread groove of rotary rod (82), and the bottom of gear (83) sets up handle (85).
6. The stem cell culture perfusion device of claim 5, wherein: the right-hand member of gear (83) is provided with tooth piece (86), and the right-hand member of tooth piece (86) is provided with inner tube (87) to tooth piece (86) distribute in the surface of inner tube (87), tooth piece (86) are provided with 6, and tooth piece (86) are located the half circle of inner tube (87).
7. The stem cell culture perfusion device of claim 6, wherein: the bottom of inner tube (87) is provided with macropores (810), and macropores (810) are provided with three groups to three groups of macropores (810) are located in general of inner tube (87), the other half of inner tube (87) bottom is the baffle, the outside of inner tube (87) is provided with urceolus (89), and the size of urceolus (89) is greater than the size of inner tube (87).
8. The stem cell culture perfusion device of claim 7, wherein: the bottom of urceolus (89) is provided with macropore (810), and macropore (810) of inner tube (87) are the same position, the other half of urceolus (89) bottom is provided with three little hole (811), and the position of little hole (811) is symmetric distribution with macropore (810), the surface of urceolus (89) is provided with channel (88), and tooth piece (86) are located the inside slip of channel (88).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320556091.1U CN219930128U (en) | 2023-03-21 | 2023-03-21 | Stem cell culture perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320556091.1U CN219930128U (en) | 2023-03-21 | 2023-03-21 | Stem cell culture perfusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219930128U true CN219930128U (en) | 2023-10-31 |
Family
ID=88503050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320556091.1U Active CN219930128U (en) | 2023-03-21 | 2023-03-21 | Stem cell culture perfusion device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219930128U (en) |
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2023
- 2023-03-21 CN CN202320556091.1U patent/CN219930128U/en active Active
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