CN219907688U - Three-dimensional stirring formula bioreactor - Google Patents
Three-dimensional stirring formula bioreactor Download PDFInfo
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- CN219907688U CN219907688U CN202320538068.XU CN202320538068U CN219907688U CN 219907688 U CN219907688 U CN 219907688U CN 202320538068 U CN202320538068 U CN 202320538068U CN 219907688 U CN219907688 U CN 219907688U
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- mixing tank
- gas
- cell
- delivery pipe
- vertical reactor
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- 238000003756 stirring Methods 0.000 title claims abstract description 54
- 238000002156 mixing Methods 0.000 claims abstract description 101
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 230000001954 sterilising effect Effects 0.000 claims abstract description 16
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 230000000149 penetrating effect Effects 0.000 claims abstract 5
- 238000007789 sealing Methods 0.000 claims description 11
- 230000001105 regulatory effect Effects 0.000 claims description 9
- 239000007789 gas Substances 0.000 description 66
- 210000004027 cell Anatomy 0.000 description 62
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 7
- 239000001301 oxygen Substances 0.000 description 7
- 229910052760 oxygen Inorganic materials 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 210000001808 exosome Anatomy 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 239000000969 carrier Substances 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- 230000000638 stimulation Effects 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000007822 coupling agent Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000003570 air Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 230000001808 coupling effect Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A three-dimensional stirring type bioreactor comprises a control host, a cell reactor base and a vertical reactor, wherein a gas mixing tank, a cell mixing tank and a heater are arranged in the control host, a sterilizing filter, a stirring motor and a liquid delivery pipe are arranged on the cell mixing tank, the cell mixing tank is connected with the vertical reactor through the liquid delivery pipe, and a liquid water separator and a peristaltic pump are sequentially arranged on the liquid delivery pipe in a penetrating manner; the gas mixing tank is provided with a gas mixing three-way valve and a gas delivery pipe, the gas mixing tank is connected with the vertical reactor through the gas delivery pipe, and the gas delivery pipe is sequentially provided with a gas dividing valve, a gas pipe fixer and a filter in a penetrating way; the vertical reactor comprises a vertical reactor tank body, an ultrasonic generator arranged on the vertical reactor tank body and a stirring impeller arranged in the vertical reactor tank body; the cell reactor base is provided with a magnetic electric device, the magnetic electric device is provided with a magnetic blade, and the magnetic blade is magnetically connected with the stirring impeller.
Description
Technical Field
The utility model relates to the field of bioreactors, in particular to a three-dimensional stirring type bioreactor.
Background
In the prior art, in the field of preparation of biopharmaceuticals and exocrine agents, most of cell culture uses a culture dish to be placed in an incubator for culture, and the culture dish is limited to a limited space in the incubator and a cell wall-attaching area of the culture dish, so that the cell culture efficiency is limited, meanwhile, the cell incubator occupies a wide area, the input cost is high, the production and operation cost is high, the cell yield is low, and the cell culture system is inconvenient to effectively stimulate the exocrine behavior of cells.
Disclosure of Invention
The utility model aims to provide a three-dimensional stirring type bioreactor which aims to solve the technical problems in the background technology.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a three-dimensional stirring formula bioreactor, includes control host computer, cell reactor base and vertical reactor, be equipped with gas mixing tank, cell mixing tank and heater in the control host computer, the cell mixing tank includes the mixing tank body, and upwards set up sealing washer and the mixing tank apron on the mixing tank body jar mouth in proper order, be equipped with degerming filter, agitator motor and liquid delivery tube on the mixing tank apron, coaxial coupling has the (mixing) shaft in the pivot of agitator motor, is equipped with stirring vane on the (mixing) shaft, and (mixing) shaft and stirring vane locate the inside of mixing tank body, be equipped with liquid water knockout drum and peristaltic pump on the liquid delivery tube, liquid delivery tube one end is in deep into mixing tank body bottom, and other one end link up in proper order and is connected liquid water knockout drum, peristaltic pump and vertical reactor; the gas mixing tank is provided with a gas mixing three-way valve and a gas delivery pipe, the gas delivery pipe is provided with a gas dividing valve, a gas pipe fixer and a filter, and the gas delivery pipe is sequentially and thoroughly connected with the gas dividing valve, the gas pipe fixer, the filter and the vertical reactor; the vertical reactor comprises a vertical reactor tank body, an ultrasonic generator arranged on the vertical reactor tank body and a stirring impeller arranged in the vertical reactor tank body, wherein a circular groove is formed in the side wall of the vertical reactor tank body, and the ultrasonic generator is embedded in the circular groove; the cell reactor is characterized in that a magnetic electric device and a fixed support are arranged on the cell reactor base, a magnetic blade is arranged on the magnetic electric device, the magnetic blade and the vertical reactor are fixed on the fixed support, and the magnetic blade is magnetically connected with the stirring impeller.
Further, the control host is also provided with a digital display screen, control keys and a power indicator lamp; the cell reactor base is provided with a rotating speed display screen and a speed regulating knob.
Furthermore, the sterilizing filter is in through connection with the inner part of the mixing tank body, and the connecting part of the sterilizing filter and the cover plate of the mixing tank is in sealed connection.
Furthermore, the liquid delivery pipe is communicated with the inside of the mixing tank body, and the liquid delivery pipe is in sealing connection with the joint of the cover plate of the mixing tank.
Furthermore, the gas mixing three-way valve and the gas delivery pipe are all in through connection with the gas mixing tank, and the joints of the gas mixing three-way valve and the gas delivery pipe are in sealing connection with the gas mixing tank.
Compared with the prior art, the utility model has the following characteristics and beneficial effects:
the utility model has the advantages of small equipment investment, reasonable and compact structure, small occupied area and low production and operation cost, can freely adjust gas components by arranging the gas mixing tank, simulate low oxygen and normal oxygen concentration conditions, create a vertical reaction environment by arranging the magnetic electric device, the magnetic blade and the stirring impeller, provide ultrasonic physical stimulation for cells, be beneficial to the generation of extracellular bodies, achieve the purposes of low cost and high yield, have the characteristics of safety, applicability and the like, have good popularization and practical value, and can generate good economic benefit after wide popularization and application.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a cell reactor according to the present utility model;
FIG. 2 is a schematic diagram of the internal piping of the cell reactor of the present utility model;
FIG. 3 is a front view of a gas mixing tank and cell mixing tank of the present utility model;
FIG. 4 is a partial cross-sectional elevation view of a cell mixing tank of the present utility model;
FIG. 5 is a partial cross-sectional elevation view of a vertical reactor of the present utility model;
FIG. 6 is a partial cross-sectional elevation view of the magnetomotive force device of the present utility model.
Reference numerals: 1. a gas mixing tank; 2. a gas mixing three-way valve; 3. a gas delivery tube; 4. a sterilizing filter; 5. a liquid delivery tube; 6. a stirring motor; 7. a liquid water separator; 8. a cell mixing tank; 9. a gas dividing valve; 10. a peristaltic pump; 11. a tracheal tube fixer; 12. a carrier; 13. a mixing tank cover plate; 14. a sealing gasket; 15. a locking bolt; 16. a mixing tank; 17. stirring blades; 18. a stirring shaft; 19. a vertical reactor; 20. a vertical reactor tank; 21. a cover; 22. a circular groove; 23. stirring impeller; 24. a magnetic electric device; 25. a fixed bracket; 26. a magnetic blade; 27. a heater; 28. a host door; 29. a control host; 30. a digital display screen; 31. a control key; 32. a work indicator light; 33. a filter; 34. an ultrasonic generator; 35. a rotation speed display screen; 36. a cell reactor base; 37. a speed regulating knob.
Description of the embodiments
The present utility model will be further described below in order to make the technical means, innovative features, achieved objects and effects achieved by the present utility model easy to understand.
The examples described herein are specific embodiments of the present utility model, which are intended to illustrate the inventive concept, are intended to be illustrative and exemplary, and should not be construed as limiting the utility model to the embodiments and scope of the utility model. In addition to the embodiments described herein, those skilled in the art can adopt other obvious solutions based on the disclosure of the claims and specification, including those adopting any obvious substitutions and modifications to the embodiments described herein.
The utility model discloses a three-dimensional stirring bioreactor, as shown in fig. 1 and 2, comprising a control host 29, a cell reactor base 36 and a vertical reactor 19, wherein the control host 29 is of a box structure, a gas mixing tank 1, a cell mixing tank 8 and a heater 27 are arranged in the control host 29, the cell mixing tank 8 comprises a mixing tank body 16, a sealing gasket 14 and a mixing tank cover plate 13 which are sequentially arranged upwards on the tank opening of the mixing tank body 16, the sealing gasket 14 and the mixing tank cover plate 13 are fixed through a locking bolt 15, a sterilizing filter 4 is arranged on the mixing tank cover plate 13 and used for filtering mixed gas entering the cell mixing tank 8, the sterilizing filter 4 is in through connection with the inside of the mixing tank body 16, the sterilizing filter 4 is in sealed connection with the mixing tank cover plate 13, a stirring motor 6 is simultaneously arranged on the mixing tank cover plate 13, a stirring shaft 18 is coaxially connected to the rotating shaft of the stirring motor 6, stirring blades 17 are arranged on the stirring shaft 18, the stirring motor 6 is sequentially arranged in the mixing tank body 16, and is sequentially transmitted to the stirring blade 17 through the stirring shaft 18, so that the torque force is transmitted to the stirring blade 17 through the mixing tank body 16, the sealing gasket 14 and the mixing tank body is sequentially connected to the liquid outlet pipe 5, the liquid outlet pipe 7 is sequentially penetrates through the liquid outlet pipe 5 and the liquid outlet pipe 7, the liquid outlet pipe 5 is connected with the liquid outlet pipe 7, the liquid outlet pipe 5 and the liquid outlet pipe 7 is connected with the liquid outlet pipe 5, the liquid outlet pipe 7 and the liquid outlet pipe 7 is sequentially arranged in the liquid outlet pipe 5; the gas mixing tank 1 is provided with a gas mixing three-way valve 2 and a gas delivery pipe 3, the gas mixing three-way valve 2 and the gas delivery pipe 3 are all in through connection with the gas mixing tank 1, the joints of the gas mixing three-way valve 2, the gas delivery pipe 3 and the gas mixing tank 1 are all in sealed connection, the gas delivery pipe 3 is provided with a gas dividing valve 9, a gas pipe fixer 11 and a filter 33, the gas delivery pipe 3 is sequentially in through connection with the gas dividing valve 9, the gas pipe fixer 11, the filter 33 and a vertical reactor 19, as shown in figures 3 and 5, the vertical reactor 19 is arranged on a cell reactor base 36, the vertical reactor 19 comprises a vertical reactor tank body 20, an ultrasonic generator 34 arranged on the vertical reactor tank body 20, and a stirring impeller 23 arranged inside the vertical reactor tank body 20, a cover 21 is arranged at the tank opening of the vertical reactor tank body 20, the vertical reactor tank body 20 is sealed, a circular groove 22 is arranged on the side wall of the vertical reactor tank body 20, an ultrasonic generator 34 is embedded in the circular groove 22, a coupling agent is coated at the embedded part of the ultrasonic generator 34 and the circular groove 22, ultrasonic waves emitted by the ultrasonic generator 34 are 1.5 MHz, intermittent frequency is 1 KHZ, a magnet is arranged in a stirring impeller 23, a magnetomotive electric device 24 and a fixed support 25 are further arranged on a cell reactor base 36, a magnetomotive blade 26 is arranged on the magnetomotive electric device 24, the magnetomotive blade 26 and the vertical reactor 19 are fixed on the fixed support 25, the magnetomotive blade 26 is magnetically connected with the stirring impeller 23, and the stirring impeller 23 with the magnet inside in the vertical reactor 19 can be driven to rotate by adjusting the rotating speed of the magnetomotive blade 26 on the magnetomotive electric device 24 through the magnetomotive coupling effect of the magnetomotive blade 26 and the stirring impeller 23.
As shown in fig. 1, the control host 29 is further provided with a digital display screen 30, control keys 31 and a power indicator lamp; the cell reactor base 36 is provided with a rotation speed display screen 35 and a speed regulating knob 37.
The functions of the parts in the utility model are described in detail as follows:
1. control host 29: the device is a comprehensive cell culture control device integrating temperature control, gas control and liquid control, and a gas mixing tank 1 and a cell mixing tank 8 are mainly arranged in an inner cavity of the device;
2. the gas mixing tank 1 is used for mixing carbon dioxide, air and nitrogen with each other, and regulating the air, the carbon dioxide and the nitrogen through the gas mixing three-way valve 2 to provide a good growth environment suitable for cell growth or exosome extraction, so that the normal oxygen partial pressure condition required by high-density growth of cells and the low oxygen environment required by enrichment of the exosome of the cells are effectively ensured;
3. cell mixing tank 8. Cell mixing tank 8 is filled with carrier 12 suitable for cell adhesion growth, cell mixing tank 8 is provided with sterilizing filter 4, stirring motor 6 and liquid delivery tube 5; the cell mixing tank 8 works on the principle that: the stirring motor 6 drives the stirring shaft 18 and the stirring blade to rotate, so that the cells, the carrier 12 and the corresponding culture medium are fully mixed, and the cells are attached to the surface of the carrier 12; then the mixed solution of the cells, the carriers 12 and the culture medium is injected into the vertical reactor 19 through the liquid delivery pipe 5, the liquid water separator 7 and the peristaltic pump 10 on the cell mixing tank 8 in sequence, so that a constant-temperature environment and a larger attachment surface are provided for the growth of the cells, and a proper environment is provided for the attachment of the cells on the surface of the carriers 12;
4. digital display screen 30: the three-dimensional stirring type bioreactor system can intuitively display the temperature, the liquid and the gas system running state of the whole device, is displayed by a digital display screen 30, and is matched with a control key 31 to control the state of each part of process flow, such as temperature conditions, three-gas mixing proportion, gas flow, liquid stirring, flow and other parameters;
5. work indicator light 32: is the work indication of each executing mechanism in the whole device;
6. cell reactor base 36 provides support for vertical reactor 19, provides rotational power for agitation impeller 23 on vertical reactor 19, and provides physical stimulation for the cultured cells through ultrasonic generator 34 above; the working principle is as follows: the cell reactor base 36 is mutually fixed with the vertical reactor 19 through a fixed bracket 25 on the cell reactor base 36, and simultaneously, the magnetic blades 26 on the magnetic electric device 24 are mutually coupled with the magnet on the stirring impeller 23 in the vertical reactor 19 to drive the stirring impeller 23 to rotate, so that cells, carriers 12, gas and culture mediums in the cavity of the vertical reactor 19 are fully mixed, proper conditions are provided for proliferation and exosome production of the cells, meanwhile, the ultrasonic generator 34 on the cell reactor base 36 is mutually embedded with the circular groove 22 on the vertical reactor 19, the coupling agent is coated on the embedded surface, and ultrasonic waves generated by the ultrasonic generator 34 are transmitted to the surface of the cells through the conduction of the vertical reactor 19, so that physical stimulation with certain intensity is given to the cells;
7. the speed regulating knob 37 on the cell reactor base 36 can regulate the rotation speed of the magnetic force blade 26 and display the rotation speed from time to time through the rotation speed display screen 35;
8. various pipes: liquid transfer lines are used to transfer the culture medium, cells and carriers 12 to the vertical reactor 19, and gas transfer lines are used to transfer the tri-gas mixture into the vertical reactor 19.
When in use, the cell mixing tank 8 is sterilized, after the sterilization, the porous loose carrier 12, cells and culture medium are placed in the cell mixing tank 8, the temperature of the heater 27 is opened and set, and meanwhile, the proportion of three gases entering the gas mixing tank 1 is adjusted to be the normal oxygen concentration through the gas mixing three-way valve 2; then, the stirring motor 6 is started to fully mix the carrier 12, the cells and the culture medium, so that the cells are attached to the carrier 12; then the peristaltic pump 10 is started to lead the mixed carrier 12, cells and culture solution to enter the vertical reactor 19 through the liquid delivery pipe 5 and the liquid water separator 7 according to the set volume; simultaneously, the gas mixed in the gas mixing tank 1 is led into the vertical reactor 19 through the gas outlet pipe 3, the gas dividing valve 9 and the filter 33; starting a speed regulation knob 37 on a cell reactor base 36 to regulate the rotation speed of a magnetic blade 26 on the magnetic electric device 24, so as to drive the stirring impeller 23 in the vertical reactor 19 to rotate; the cells in the vertical reactor 19 are subjected to three-dimensional culture under the drive of the stirring impeller 23; after culturing for a period of time, the cells are continuously proliferated on the surface of the carrier 12 to reach the limit number, the total number of the cells is not increased, the mixed gas three-way valve 2 is regulated to reduce the oxygen content to a low oxygen state, simultaneously the ultrasonic generator 34 is turned on and embedded in the circular groove 22 on the three-dimensional reactor coated with the coupling agent, the cells in the ultrasonic generator are subjected to physical stimulation, after waiting for a certain time, the culture medium supernatant in the vertical reactor 19 is collected, and the exosomes are obtained through centrifugal separation.
Culturing mesenchymal stem cells and obtaining exosomes, and mainly comprises the following steps:
step 1, sterilization: taking out the cell mixing tank 8, tightly wrapping all pipe orifices by clean cloth, and sterilizing in a high-pressure steam sterilization device at 121 ℃ for 30min; the carrier 12 was sterilized by irradiation at a dose of 12KGy.
Step 2, inoculating: in a clean room, 3X 108 bone marrow mesenchymal stem cells, 20g of carrier 12 and 2L of alpha-MEM complete medium (containing 1% diabody and 10% fetal bovine serum) were added to cell mixing tank 8.
Step 3, connecting pipelines: in a ten-thousand-level clean environment, the cell mixing tank 8, the sterilizing filter 4 and the liquid delivery pipe 5 are connected by a silica gel pipe through aseptic operation, and the other end of the liquid delivery pipe 5 is connected with each other through a peristaltic pump 10 and a vertical reactor 19; the gas outlet pipe 3 of the gas flow line is connected to a filter 33 provided in the vertical reactor 19, and the mixed gas is introduced from the gas mixing tank 1 into the vertical reactor 19.
Step 4, setting a control host 29 to enable the ambient temperature of the gas mixing tank 1 and the cell mixing tank 8 to be 37 ℃; the volume concentration of CO2 in the culture gas mixed by the gas mixing three-way valve 2 is 5%, the volume concentration of O2 is 21%, and the culture gas is stirred at a normal speed of 30RPM after the stirring motor is started for slowly stirring at 65RPM for 4 hours.
Step 5, culturing in a vertical reactor 19: the mixed cells, carrier 12 and alpha-MEM complete medium are transported to a vertical reactor 19 through a liquid delivery pipe 5, through a liquid water separator 7, a speed regulating knob 37 is started, the rotation speed of a magnetic blade 26 on a magnetic electric device 24 is regulated to 40RPM, after 5 days of culture, the reactor is stopped, and the supernatant is taken to extract stem cell exosomes.
The foregoing description of the preferred embodiments of the utility model is not intended to limit the utility model to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the utility model are intended to be included within the scope of the utility model.
Claims (9)
1. The utility model provides a three-dimensional stirring formula bioreactor, includes control host computer (29), cell reactor base (36) and vertical reactor (19), its characterized in that: the device is characterized in that a gas mixing tank (1), a cell mixing tank (8) and a heater (27) are arranged in the control host (29), a sterilizing filter (4), a stirring motor (6) and a liquid delivery pipe (5) are arranged on the cell mixing tank (8), the cell mixing tank (8) is connected with a vertical reactor (19) through the liquid delivery pipe (5), and a liquid water separator (7) and a peristaltic pump (10) are sequentially arranged on the liquid delivery pipe (5) in a penetrating manner; the gas mixing tank (1) is provided with a gas mixing three-way valve (2) and a gas delivery pipe (3), the gas mixing tank (1) is connected with the vertical reactor (19) through the gas delivery pipe (3), and the gas delivery pipe (3) is sequentially provided with a gas dividing valve (9), a gas pipe fixer (11) and a filter (33) in a penetrating way; the vertical reactor (19) comprises a vertical reactor tank body (20), an ultrasonic generator (34) arranged on the vertical reactor tank body (20) and a stirring impeller (23) arranged inside the vertical reactor tank body (20); the cell reactor is characterized in that a magnetic electric device (24) is arranged on the cell reactor base (36), a magnetic blade (26) is arranged on the magnetic electric device (24), and the magnetic blade (26) is magnetically connected with the stirring impeller (23).
2. A three-dimensional stirred bioreactor as in claim 1 wherein: the cell mixing tank (8) comprises a mixing tank body (16), and a sealing gasket (14) and a mixing tank cover plate (13) which are sequentially arranged on the tank opening of the mixing tank body (16) upwards.
3. A three-dimensional stirred bioreactor as in claim 1 wherein: the cell reactor base (36) is provided with a fixed bracket (25), and the magnetic blades (26) and the vertical reactor (19) are fixed on the fixed bracket (25).
4. A three-dimensional stirred bioreactor as in claim 1 wherein: the control host (29) is also provided with a digital display screen (30), control keys (31) and a power indicator lamp; the cell reactor base (36) is provided with a rotating speed display screen (35) and a speed regulating knob (37).
5. The three-dimensional stirred tank bioreactor of claim 4, wherein: the stirring motor (6) is coaxially connected with a stirring shaft (18) on a rotating shaft, and stirring blades (17) are arranged on the stirring shaft (18).
6. A three-dimensional stirred bioreactor as in claim 2 wherein: the sterilizing filter (4) is communicated with the inside of the mixing tank body (16), and the sterilizing filter (4) is connected with the cover plate (13) of the mixing tank in a sealing way.
7. A three-dimensional stirred bioreactor as in claim 2 wherein: the liquid delivery pipe (5) is connected with the inside of the mixing tank body (16) in a penetrating way, and the liquid delivery pipe (5) is connected with the cover plate (13) of the mixing tank in a sealing way.
8. A three-dimensional stirred bioreactor as in claim 2 wherein: the gas mixing three-way valve (2), the gas delivery pipe (3) and the gas mixing tank (1) are all in through connection, and the gas mixing three-way valve (2), the gas delivery pipe (3) and the gas mixing tank (1) are all in sealing connection.
9. The stereoscopic stirred bioreactor according to any one of claims 1 to 8, wherein: a circular groove (22) is formed in the side wall of the vertical reactor tank body (20), and an ultrasonic generator (34) is embedded in the circular groove (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320538068.XU CN219907688U (en) | 2023-03-20 | 2023-03-20 | Three-dimensional stirring formula bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320538068.XU CN219907688U (en) | 2023-03-20 | 2023-03-20 | Three-dimensional stirring formula bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219907688U true CN219907688U (en) | 2023-10-27 |
Family
ID=88426383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320538068.XU Active CN219907688U (en) | 2023-03-20 | 2023-03-20 | Three-dimensional stirring formula bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219907688U (en) |
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2023
- 2023-03-20 CN CN202320538068.XU patent/CN219907688U/en active Active
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