CN219861327U - Reaction tube for tubular nucleic acid extraction - Google Patents
Reaction tube for tubular nucleic acid extraction Download PDFInfo
- Publication number
- CN219861327U CN219861327U CN202320534117.2U CN202320534117U CN219861327U CN 219861327 U CN219861327 U CN 219861327U CN 202320534117 U CN202320534117 U CN 202320534117U CN 219861327 U CN219861327 U CN 219861327U
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- reaction
- acid extraction
- tubular
- reaction chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 121
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 91
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 91
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 91
- 238000000605 extraction Methods 0.000 title claims abstract description 44
- 239000002699 waste material Substances 0.000 claims abstract description 51
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 238000002347 injection Methods 0.000 claims abstract description 14
- 239000007924 injection Substances 0.000 claims abstract description 14
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 abstract description 5
- 230000007547 defect Effects 0.000 abstract description 3
- 238000003745 diagnosis Methods 0.000 abstract description 3
- 238000000338 in vitro Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of in-vitro diagnosis, in particular to a reaction tube for tubular nucleic acid extraction, which comprises a reaction tube body and a waste liquid tube body, wherein a reaction chamber is formed in the interior of the reaction tube body in a hollow manner, the reaction chamber is used for containing reaction liquid, an injection port for injecting the reaction liquid is arranged in the reaction chamber, one end of the waste liquid tube body is communicated with the reaction chamber, the other end of the waste liquid tube body is used for being connected with a waste liquid tube of a nucleic acid extraction instrument, and waste liquid formed after the reaction of the reaction liquid in the reaction chamber is completely discharged through the waste liquid tube body by sucking air through the waste liquid tube. Can be matched with a nucleic acid extractor to realize automatic tubular nucleic acid extraction, overcomes the defect that waste liquid in the nucleic acid extraction process needs to be sucked out by using a disposable suction head singly in the prior art, can effectively save the consumable usage amount of the automatic nucleic acid extractor, reduces the volume of the automatic nucleic acid extractor, and simultaneously provides a solution for automatic tubular nucleic acid extraction on the automatic nucleic acid extractor.
Description
Technical Field
The utility model relates to the technical field of in-vitro diagnosis, in particular to a reaction tube for tubular nucleic acid extraction.
Background
In vitro diagnosis refers to products and services for detecting human body samples (blood, body fluid, tissue, etc.) outside the human body to obtain clinical diagnosis information and further judging diseases or body functions.
One of the key steps in detecting human samples is the purification of nucleic acids (DNA and RNA) in the sample. The method of nucleic acid purification is an important factor affecting the quality of the extracted nucleic acid, and only high-quality nucleic acid can meet various downstream applications, and the extracted high-quality nucleic acid can be directly applied to PCR (polymerase chain reaction) experiments or other molecular biology experiments.
In the process of extracting nucleic acid, a nucleic acid extractor and related consumable materials are required to be matched, and the nucleic acid extractor on the market at present mainly takes a plate type as a main part and is divided into: the types of 8, 16, 24, 32, 48 and 96 are all matched with 96 deep hole plates and magnetic rod sleeves as consumable materials, and the consumable materials which are tubular for extraction and can be matched with an automatic nucleic acid extractor for use are not common. Thus, there is a need for a reaction tube for tubular nucleic acid extraction to address the nucleic acid extraction reaction in automated tubular nucleic acid extraction.
Disclosure of Invention
In view of the above-mentioned drawbacks of the prior art, the present utility model is to provide a reaction tube for tubular nucleic acid extraction, which can be used with a nucleic acid extractor to realize automated tubular nucleic acid extraction.
In order to solve the technical problems, the utility model adopts the following technical scheme:
the utility model provides a reaction tube for tubular nucleic acid extraction, which comprises a reaction tube body and a waste liquid tube body, wherein a reaction chamber is formed in the hollow part of the reaction tube body, the reaction chamber is used for containing reaction liquid, an injection port for injecting the reaction liquid is arranged in the reaction chamber, one end of the waste liquid tube body is communicated with the reaction chamber, the other end of the waste liquid tube body is used for being connected with a waste liquid tube of a nucleic acid extractor, and waste liquid formed after the reaction of the reaction liquid in the reaction chamber is completely discharged through the waste liquid tube body through air suction of the waste liquid tube.
Preferably, one end of the liquid discarding pipe body, which is communicated with the reaction chamber, extends into one end of the reaction chamber, which is far away from the injection port, and a gap is reserved between the liquid discarding pipe body and one end of the reaction chamber, which is far away from the injection port.
Preferably, the spacing gap is 0.2-0.8mm.
Preferably, one end of the waste tube used for connecting the nucleic acid extractor is provided with a waste outlet, and the waste outlet is arranged on the reaction tube.
Preferably, the injection port and the waste disposal port are arranged on the same end face of the reaction tube body.
Preferably, the liquid discarding pipe is arranged in the reaction chamber.
Preferably, the liquid discarding pipe body is fixed on the side wall of the reaction chamber.
Preferably, the reaction tube is provided with a fixing portion for connecting to the nucleic acid extractor outside the reaction chamber.
Preferably, two fixing parts are arranged, and the two fixing parts are respectively positioned at two sides of the injection port of the reaction chamber.
Preferably, the nucleic acid extracting apparatus has a flow rail, and the fixing portion includes a first fixing groove and a second fixing groove for respectively connecting and fixing an upper side and a lower side of the flow rail.
Compared with the prior art, the utility model has obvious progress:
the reaction tube for tubular nucleic acid extraction can be matched with a nucleic acid extractor to realize automatic tubular nucleic acid extraction, waste liquid in a reaction chamber of the reaction tube body can be automatically discharged by the waste liquid tube body through waste liquid tube suction of the automatic nucleic acid extractor, the defect that waste liquid in the nucleic acid extraction process needs to be sucked out by a disposable suction head singly in the prior art is overcome, the consumption of consumable materials of the automatic nucleic acid extractor can be effectively saved, the volume of the automatic nucleic acid extractor is reduced, meanwhile, a solution is provided for automatic tubular nucleic acid extraction on the automatic nucleic acid extractor, and the possibility is provided for the development of a follow-up flow type nucleic acid detection platform.
Drawings
FIG. 1 is a schematic perspective view of a reaction tube for tubular nucleic acid extraction according to an embodiment of the present utility model.
FIG. 2 is a schematic semi-sectional view of a reaction tube for tubular nucleic acid extraction according to an embodiment of the present utility model.
Wherein reference numerals are as follows:
the utility model is illustrated, but not limited, by the following claims.
Detailed Description
In the description of the present utility model, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
Furthermore, in the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.
As shown in FIGS. 1 and 2, one example of a reaction tube for tubular nucleic acid extraction of the present utility model.
The reaction tube for tubular nucleic acid extraction of the present embodiment includes a reaction tube body 1 and a discard solution tube body 2. The reaction tube 1 has a hollow interior to form a reaction chamber 10, and the reaction chamber 10 is provided with an inlet 11 for injecting a reaction liquid. The reaction chamber 10 is used for containing a reaction solution to perform a nucleic acid extraction reaction, thereby realizing tubular nucleic acid extraction. One end of the waste liquid pipe body 2 is communicated with the reaction chamber 10, the other end of the waste liquid pipe body 2 is used for being connected with a waste liquid removing pipe of the nucleic acid extraction instrument, and waste liquid formed after the reaction of the reaction liquid in the reaction chamber 10 is completed is discharged through the waste liquid removing pipe body 2 by sucking air through the waste liquid removing pipe of the nucleic acid extraction instrument. The nucleic acid extractor is the existing equipment, and the automatic nucleic acid extractor is provided with a waste discarding pipe capable of automatically sucking air, so that the waste liquid in the reaction chamber 10 of the reaction pipe body 1 can be automatically discharged.
Therefore, the reaction tube for tubular nucleic acid extraction of the embodiment can be matched with a nucleic acid extractor to realize automatic tubular nucleic acid extraction, waste liquid in the reaction chamber 10 of the reaction tube body 1 can be automatically discharged by the waste liquid tube body 2 through waste liquid suction of the automatic nucleic acid extractor, the defect that waste liquid in the nucleic acid extraction process needs to be sucked out by a disposable suction head in the prior art is overcome, the consumption of consumable materials of the automatic nucleic acid extractor can be effectively saved, the volume of the automatic nucleic acid extractor is reduced, meanwhile, a solution is provided for automatic tubular nucleic acid extraction on the automatic nucleic acid extractor, and the possibility is provided for the development of a follow-up flow type nucleic acid detection platform.
In this embodiment, preferably, one end of the liquid-discarding tube 2 communicating with the reaction chamber 10 of the reaction tube 1 extends into one end of the reaction chamber 10 away from the injection port 11, and a gap is left between one end of the liquid-discarding tube 2 communicating with the reaction chamber 10 of the reaction tube 1 and one end of the reaction chamber 10 away from the injection port 11, where the gap is 0.2-0.8mm, preferably 0.5mm. Thereby, the waste liquid in the reaction chamber 10 can be discharged as much as possible, and the circulation of the waste liquid is facilitated.
In this embodiment, preferably, one end of the waste tube 2 for connecting the waste tube of the nucleic acid extractor is provided with a waste port 21, and the waste port 21 is provided on the reaction tube 1, so that the fixing of the waste tube 2 to the reaction tube 1 and the connection of the waste tube 2 to the waste tube of the nucleic acid extractor are facilitated.
Further, for convenience of operation, the inlet 11 of the reaction chamber 10 of the reaction tube 1 and the reject outlet 21 of the reject tube 2 are provided on the same end face of the reaction tube 1.
In this embodiment, the liquid-discarding tube 2 is preferably built into the reaction chamber 10 of the reaction tube 1. Preferably, the reject tube 2 is fixed to the side wall of the reaction chamber 10.
In this embodiment, the reaction tube 1 is preferably provided with a fixing portion 12 for connecting to a nucleic acid extractor outside the reaction chamber 10. When the reaction tube-based nucleic acid extractor for tubular nucleic acid extraction of the present embodiment is used, the reaction tube of the present embodiment can be fixedly mounted on the nucleic acid extractor by connecting the fixing portion 12 on the reaction tube body 1 to the nucleic acid extractor. In general, an automated nucleic acid isolation instrument has a flow path, and the fixing portion 12 of the reaction tube 1 is preferably fixed to the flow path of the nucleic acid isolation instrument.
In order to increase the stability of the reaction tube of the present embodiment in assembling on the nucleic acid extractor, preferably, two fixing portions 12 are provided on the reaction tube body 1, and the two fixing portions 12 are respectively located at two sides of the injection port 11 of the reaction chamber 10, and are connected and fixed with the nucleic acid extractor through the two fixing portions 12, so as to ensure the fixation on the nucleic acid extractor of the reaction tube.
In order to facilitate the connection and fixation of the fixing portion 12 on the reaction tube 1 to the flow rail of the nucleic acid extractor, it is preferable that the fixing portion 12 on the reaction tube 1 includes a first fixing groove 121 for connection and fixation with an upper side of the flow rail of the nucleic acid extractor and a second fixing groove 122 for connection and fixation with a lower side of the flow rail of the nucleic acid extractor. The first fixing groove 121 and the second fixing groove 122 are connected and fixed with the flowing track of the nucleic acid extracting apparatus in a plugging and clamping manner, and have the advantage of convenient disassembly and assembly.
Referring to fig. 1 and 2, in a preferred embodiment, the reaction tube is vertically disposed up and down in use, the reaction tube body 1, the reaction chamber 10 and the waste liquid removing tube body 2 all extend in the up-down direction, and the injection port 11 of the reaction chamber 10 of the reaction tube body 1 and the waste liquid removing port 21 of the waste liquid removing tube body 2 are both disposed on the upper end surface of the reaction tube body 1; the two fixing parts 12 are respectively positioned at two sides of the injection port 11 of the reaction chamber 10, namely at two sides of the upper end of the reaction tube body 1, and the upper end surfaces of the two fixing parts 12 are flush with the upper end surface of the reaction tube body 1, so that the reaction tube body 1 is in a T shape as a whole; each fixing portion 12 includes a first fixing groove 121 and a second fixing groove 122, the first fixing groove 121 is a groove formed by downwardly recessing the upper surface of the fixing portion 12, the second fixing groove 122 is a groove formed by upwardly recessing the lower surface of the fixing portion 12, the recessing depth of the first fixing groove 121 is smaller than that of the second fixing groove 122, and the first fixing groove 121 and the second fixing groove 122 are not communicated with each other.
The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that modifications and substitutions can be made by those skilled in the art without departing from the technical principles of the present utility model, and these modifications and substitutions should also be considered as being within the scope of the present utility model.
Claims (10)
1. A reaction tube for tubular nucleic acid extraction, its characterized in that includes reaction tube body (1) and abandons liquid body (2), reaction tube body (1) inside cavity forms reaction chamber (10), reaction chamber (10) are used for holding the reaction liquid, reaction chamber (10) are equipped with and are used for injecting filling port (11) of reaction liquid, abandon liquid body (2) one end with reaction chamber (10) are linked together, abandon the other end of liquid body (2) and be used for connecting the abandoning useless pipe of nucleic acid extraction appearance, through abandoning useless pipe inspiration messenger in reaction chamber (10) waste liquid that forms after the reaction liquid reaction is accomplished is discharged via abandoning liquid body (2).
2. The reaction tube for tubular nucleic acid extraction according to claim 1, wherein an end of the discard tube body (2) communicating with the reaction chamber (10) extends into an end of the reaction chamber (10) away from the injection port (11) and a gap is left between the discard tube body and an end of the reaction chamber (10) away from the injection port (11).
3. The reaction tube for tubular nucleic acid extraction of claim 2, wherein the gap is 0.2-0.8mm.
4. The reaction tube for tubular nucleic acid extraction according to claim 1, wherein one end of the waste tube (2) for connecting the nucleic acid extractor is provided with a waste outlet (21), and the waste outlet (21) is provided on the reaction tube (1).
5. The reaction tube for tubular nucleic acid extraction according to claim 4, wherein the inlet (11) and the reject port (21) are provided on the same end face of the reaction tube body (1).
6. The reaction tube for tubular nucleic acid extraction according to claim 1, characterized in that the reject tube body (2) is built into the reaction chamber (10).
7. The reaction tube for tubular nucleic acid extraction according to claim 6, characterized in that the reject tube body (2) is fixed on a side wall of the reaction chamber (10).
8. The reaction tube for tubular nucleic acid extraction according to claim 1, wherein a fixing portion (12) for connecting the nucleic acid extractor is provided on the reaction tube body (1) outside the reaction chamber (10).
9. The reaction tube for tubular nucleic acid extraction according to claim 8, wherein two of the fixing portions (12) are provided, the two fixing portions (12) being located on both sides of the injection port (11) of the reaction chamber (10), respectively.
10. The reaction tube for tubular nucleic acid extraction according to claim 8, wherein the nucleic acid extraction instrument has a flow rail, and the fixing portion (12) includes a first fixing groove (121) and a second fixing groove (122) for respectively connecting and fixing an upper side and a lower side of the flow rail.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320534117.2U CN219861327U (en) | 2023-03-17 | 2023-03-17 | Reaction tube for tubular nucleic acid extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320534117.2U CN219861327U (en) | 2023-03-17 | 2023-03-17 | Reaction tube for tubular nucleic acid extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219861327U true CN219861327U (en) | 2023-10-20 |
Family
ID=88319100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320534117.2U Active CN219861327U (en) | 2023-03-17 | 2023-03-17 | Reaction tube for tubular nucleic acid extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219861327U (en) |
-
2023
- 2023-03-17 CN CN202320534117.2U patent/CN219861327U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR960000025B1 (en) | Medical micro pipette tips for difficult to reach places and related methods | |
| JP5984616B2 (en) | Comolded stabable stopper and its manufacturing method | |
| JP2006181345A (en) | Double take-off needle and method for producing it | |
| CN112300911A (en) | Nucleic acid detector and nucleic acid detection method | |
| US20070163366A1 (en) | Device and method for pre-treating and injecting liquid specimen | |
| CN219861327U (en) | Reaction tube for tubular nucleic acid extraction | |
| CN111172008B (en) | Method and device for automatic nucleic acid extraction | |
| CN213856203U (en) | Biochemical reaction cup cleaning module and cleaning device | |
| CN219314928U (en) | Embryo culture dish | |
| CN111659477A (en) | Independent sample introduction micro-fluidic immunoassay joint inspection device | |
| CN111690506A (en) | Nucleic acid extraction equipment | |
| CN115029210A (en) | Closed type rapid nucleic acid extraction device and extraction method adopting one-step method | |
| CN219374719U (en) | Multi-purpose collector of amniotic fluid puncture | |
| CN219306780U (en) | Urine collection bottle convenient for assaying and sampling | |
| CN217289628U (en) | Puncture needle self-cleaning mechanism for digital PCR | |
| CN218666055U (en) | Detection kit | |
| CN110711614A (en) | Multi-channel biological reaction device with micro-fluidic or nano-fluidic structure | |
| CN218340217U (en) | Glue filling system for gene sequencer | |
| CN210019424U (en) | Medical sampling device for preventing secondary pollution | |
| CN215611667U (en) | Pipette suction head | |
| CN220175117U (en) | Micro blood sampling straw | |
| CN219130198U (en) | Liquid device for removing outer wall of sampling needle of glycosylated hemoglobin instrument | |
| TW202017632A (en) | Method and device of automatic nucleic acid extraction | |
| CN219629666U (en) | Saliva sample collection and preservation device | |
| CN218860690U (en) | Microinjection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |