CN219830754U

CN219830754U - Immune cell counting device

Info

Publication number: CN219830754U
Application number: CN202321312894.9U
Authority: CN
Inventors: 张金龙; 艾鹏; 刘榴
Original assignee: Shanghai Danrui Biomedical Technology Co ltd
Current assignee: Shanghai Danrui Biomedical Technology Co ltd
Priority date: 2023-05-29
Filing date: 2023-05-29
Publication date: 2023-10-13
Anticipated expiration: 2033-05-29

Abstract

The utility model relates to the technical field of laboratory equipment and discloses an immune cell counting device which comprises a bottom plate, wherein a workbench is arranged on the surface of the bottom plate, a vertical plate is arranged on the upper surface of the bottom plate, a detection table is fixedly connected to the upper surface of the vertical plate, a display screen is arranged on the surface of the detection table, an observation barrel is arranged on the upper surface of the detection table, an acquisition shooting barrel is fixedly connected to the lower surface of the detection table, a zooming barrel is arranged at the lower end of the acquisition shooting barrel, a zoom lens is arranged on the inner wall of the zooming barrel, a culture dish body is arranged on the upper surface of the workbench corresponding to the acquisition shooting barrel, a vertical plate is fixedly connected to one side of the culture dish body, a square groove is formed in the surface of the vertical plate, a screw rod is rotationally connected to the inner wall of the square groove, and a handle is fixedly connected to the upper end of the screw rod. The utility model has the advantage that the focal length can be adjusted without frequent observation of the microscope lens by a worker.

Description

Immune cell counting device

Technical Field

The utility model relates to the technical field of laboratory equipment, in particular to an immune cell counting device.

Background

Cell counting is necessary in life science and medical field, immune cells are the material basis of immune function, immune cells are classified into lymphocyte, macrophage, granulocyte and the like, and the counting of immune cells is used for more accurately evaluating the immune function of an organism so as to judge the intensity of the immunity of the organism.

The current cell counting method is mainly divided into two types, namely an electrical impedance method and an image recognition method, wherein the electrical impedance method has high cost and high equipment price, so that the image recognition method is mainly used at present, and is used for shooting the cells in a culture dish by using a microscopic camera, counting the cells by using an artificial intelligence algorithm and marking the cell positions in the image.

However, in the prior art, when performing image recognition cell counting, because the sizes of different immune cells are different, when performing microscopic photography on culture dishes of different immune cells, related personnel are often required to adjust the focal length of a microscopic camera so as to avoid that the immune cells are too small to cause that the camera cannot completely acquire the whole cell distribution image, therefore, when counting different immune cell culture dishes, the personnel need to observe from a microscope opening for many times and adjust the focal length simultaneously, and therefore, the utility model provides an immune cell counting device which does not need to observe a microscopic lens by the personnel and adjust the focal length simultaneously.

Disclosure of Invention

In order to overcome the defects in the prior art, the utility model provides an immune cell counting device which has the advantage that a worker can adjust the focal length without frequently observing a microscope lens.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides an immune cell counting assembly, includes the bottom plate, the bottom plate surface is provided with the workstation, surface mounting has the riser on the bottom plate, the last fixed surface of riser is connected with the detection platform, the surface mounting who detects the platform has the display screen, the last surface mounting who detects the platform has an observation section of thick bamboo, the lower fixed surface that detects the platform is connected with gathers a section of thick bamboo of making a video recording, gather the lower extreme of making a video recording the section of thick bamboo and install a section of thick bamboo that zooms, the inner wall of a zoom section of thick bamboo is installed, the workstation upper surface corresponds the position of gathering a section of thick bamboo of making a video recording and is provided with the culture dish body, one side fixedly connected with riser of culture dish body, square groove has been seted up on the surface of riser, square groove inner wall rotation is connected with the screw rod, the upper end fixedly connected with handle of screw rod, the inner wall sliding connection of square groove has the slider, slider and screw rod threaded connection.

As a preferable technical scheme of the utility model, two movable grooves are formed in the inner wall of the acquisition camera shooting barrel, a movable plate is connected to the inner wall of the movable groove in a sliding manner, the lower end of the movable plate is fixedly connected with the zoom barrel, a rotating ring is rotatably connected to the arc surface of the acquisition camera shooting barrel, a plurality of rings of dense thread grooves are formed in the inner wall of the rotating ring, and the outer wall of the movable plate is meshed with the thread grooves in the inner wall of the rotating ring.

As a preferable technical scheme of the utility model, one side of the sliding block is fixedly connected with a limiting ring, the upper surface of the limiting ring is fixedly connected with an anti-slip pad, and the anti-slip pad is a rubber pad.

As a preferable technical scheme of the utility model, one side of the surface of the vertical plate, which is close to the square groove, is provided with scale marks, and the length of the scale marks is equal to the length of the square groove.

As a preferable technical scheme of the utility model, one side of the vertical plate is fixedly connected with a marking plate.

As a preferable technical scheme of the utility model, one side of the bottom plate is fixedly connected with an auxiliary plate, the surface of the auxiliary plate is provided with a placing groove, and the inner wall of the placing groove is provided with a plurality of culture dish bodies.

As a preferable technical scheme of the utility model, a positioning ring piece is fixedly connected to the upper surface of the workbench near the lower end of the acquisition camera cylinder, the positioning ring piece is semicircular, and the positioning ring piece is a transparent ring piece.

As a preferable technical scheme of the utility model, a plurality of illuminating lamps are arranged on the inner wall of the workbench.

Compared with the prior art, the utility model has the following beneficial effects:

according to the utility model, the vertical plate is arranged on one side of the culture dish body, so that the rotating handle is arranged according to the size of immune cells in an immune cell sample placed in the culture dish, the sliding block slides to the corresponding height, the height can be obtained through experiments in advance, the height of the sliding block can be synchronously adjusted when the immune cells are dyed in the culture dish, then when the immune cells are required to be counted, the culture dish body is placed under the collecting camera cylinder, focusing and amplifying can be rapidly completed by moving the zoom cylinder downwards to the position of the sliding block, a worker does not need to observe through the observation cylinder, and meanwhile, the zooming is adjusted, so that the time is effectively saved.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a schematic view showing the structure of the upper end of the culture dish body according to the present utility model;

FIG. 3 is an enlarged view of the utility model at A in FIG. 2;

FIG. 4 is a cross-sectional view showing the internal structure of the pick-up cartridge of the present utility model;

fig. 5 is a schematic view showing the auxiliary plate and its surface structure according to the present utility model.

In the figure: 1. a bottom plate; 11. a work table; 12. a lighting lamp; 13. a vertical plate; 14. a detection table; 15. a display screen; 16. an observation tube; 2. collecting a camera shooting barrel; 21. a rotating ring; 22. a movable groove; 221. a movable plate; 222. a zoom cylinder; 223. a zoom lens; 3. a culture dish body; 31. a riser; 32. a square groove; 321. a screw; 322. a handle; 323. a slide block; 324. a limiting ring; 325. an anti-slip pad; 33. a marking plate; 34. scale marks; 35. a positioning ring member; 4. an auxiliary plate; 41. and (5) placing a groove.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1 to 5, the utility model provides an immune cell counting device, which comprises a bottom plate 1, a workbench 11 is arranged on the surface of the bottom plate 1, a vertical plate 13 is arranged on the upper surface of the bottom plate 1, a detection table 14 is fixedly connected to the upper surface of the vertical plate 13, a display screen 15 is arranged on the surface of the detection table 14, an observation cylinder 16 is arranged on the upper surface of the detection table 14, an acquisition shooting cylinder 2 is fixedly connected to the lower surface of the detection table 14, the acquisition shooting cylinder 2 is connected with the observation cylinder 16, a zooming cylinder 222 is arranged at the lower end of the acquisition shooting cylinder 2, a zooming lens 223 is arranged on the inner wall of the zooming cylinder 222, a culture dish body 3 is arranged on the upper surface of the workbench 11 and corresponds to the position of the acquisition shooting cylinder 2, a vertical plate 31 is fixedly connected to one side of the culture dish body 3, a square groove 32 is arranged on the surface of the vertical plate 31, a screw 321 is rotatably connected to the inner wall of the square groove 32, a handle 322 is fixedly connected to the upper end of the screw 321, a sliding block 323 is slidingly connected to the inner wall of the square groove 32, and the sliding block 323 is in threaded connection with the screw 321.

The working principle and beneficial effects of the technical scheme are as follows: setting up twist grip 322, make slider 323 slide to corresponding height, this height can be obtained through the experiment in advance, when dyeing immune cells in the culture dish, can synchronous adjustment slider 323's height, when later need count immune cells, place culture dish body 3 in gathering the below of making a video recording section of thick bamboo 2, through moving down a zoom section of thick bamboo 222 to slider 323 position, can accomplish focusing fast and enlarge, need not the staff and observe through observing section of thick bamboo 16 in addition, and adjust simultaneously and zoom, effectively save time.

As shown in fig. 2 and 4, in this embodiment, two movable grooves 22 are formed in the inner wall of the capturing and photographing cylinder 2, a movable plate 221 is slidably connected to the inner wall of the movable groove 22, the lower end of the movable plate 221 is fixedly connected to the zoom cylinder 222, the circular arc surface of the capturing and photographing cylinder 2 is rotatably connected to a rotating ring 21, a plurality of rings of dense thread grooves are formed in the inner wall of the rotating ring 21, and the outer wall of the movable plate 221 is meshed with the thread grooves in the inner wall of the rotating ring 21.

Through the arrangement of a plurality of dense thread grooves, when the rotating ring 21 is rotated, the thread grooves can be synchronously rotated, and the movable plate 221 meshed with the thread grooves is matched, so that the movable plate 221 moves up and down to drive the zoom lens 223 to move up and down so as to adjust the focal length, and the movable plate 221 moves up and down more slowly and accurately due to the fact that the thread grooves are denser.

As shown in fig. 2 and 3, in the present embodiment, a limiting ring 324 is fixedly connected to one side of a slider 323, an anti-slip pad 325 is fixedly connected to an upper surface of the limiting ring 324, and the anti-slip pad 325 is a rubber pad.

Through the setting of spacing ring 324, be used for carrying out the spacing to the zoom section of thick bamboo 222 that the top moved down, make zoom section of thick bamboo 222 butt to anti-skidding pad 325 surface, improve zoom section of thick bamboo 222 adjustment accuracy.

Wherein, scale marks 34 are arranged on one side of the surface of the vertical plate 31, which is close to the square groove 32, and the arrangement length of the scale marks 34 is equal to the length of the square groove 32.

By the arrangement of the graduation marks 34, they serve as a reference when adjusting the position of the slider 323, so that the adjustment is more precise.

In addition, a marking plate 33 is fixedly connected to one side of the vertical plate 31.

By the arrangement of the marking plate 33, the immune cell type in the culture dish body 3 can be recorded at the position of the marking plate 33, so that the differentiation is convenient.

As shown in fig. 5, an auxiliary plate 4 is fixedly connected to one side of the bottom plate 1, a placement groove 41 is formed in the surface of the auxiliary plate 4, and a plurality of culture dish bodies 3 are placed on the inner wall of the placement groove 41.

Through the setting of accessory plate 4, be convenient for place a plurality of culture dish bodies 3 and reserve.

As shown in fig. 2, a positioning ring member 35 is fixedly connected to the upper surface of the workbench 11 near the lower end of the capturing cylinder 2, the positioning ring member 35 is in a semicircular shape, and the positioning ring member 35 is a transparent ring member.

Through setting up of holding ring spare 35 for when placing culture dish body 3, fix a position culture dish body 3, ensure the comprehensiveness of shooting the count.

As shown in fig. 1, a plurality of illumination lamps 12 are installed on the inner wall of the table 11.

Through the setting of a plurality of lights 12 for the illumination of workstation 11 bottom, be convenient for take a picture the count to immune cells in the culture dish.

The working principle and the using flow of the utility model are as follows:

proper immune cells are cultured in the culture dish body 3, marks are made on the marking plate 33 according to the type, the size and the like of the immune cells, according to the marks, the adjusting handle 322 rotates, the screw 321 synchronously rotates, the sliding block 323 and the limiting ring 324 are driven to move up and down, and the adjusting is carried out by referring to the scale mark 34, after all the adjusting is finished, after the cell liquid in the culture dish body 3 is dyed, the cell liquid can be placed on the surface of the workbench 11 for counting, the culture dish body 3 is abutted against the positioning ring piece 35 for positioning, then the rotating ring 21 is rotated, the zoom cylinder 222 is adjusted to move downwards until the zoom cylinder 222 is abutted against the anti-slip pad 325 on the surface of the limiting ring 324, and then the cell surface image acquisition and counting can be carried out.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An immune cell counting device comprising a base plate (1), characterized in that: the utility model provides a portable electronic device, including bottom plate (1), bottom plate (1) surface is provided with workstation (11), surface mounting has riser (13) on bottom plate (1), the last fixed surface of riser (13) is connected with detects platform (14), the surface mounting who detects platform (14) has display screen (15), the last surface mounting who detects platform (14) has observation section of thick bamboo (16), the lower fixed surface who detects platform (14) is connected with collection section of thick bamboo (2) of making a video recording, the lower extreme that gathers section of thick bamboo (2) of making a video recording is installed and is zoomed section of thick bamboo (222), zoom lens (223) are installed to the inner wall that zooms section of thick bamboo (222), the position that workstation (11) upper surface corresponds to gather section of thick bamboo (2) of thick bamboo of making a video recording is provided with culture dish body (3), one side fixedly connected with riser (31) of culture dish body (3), square groove (32) have been seted up on the surface of riser (31), square groove (32) inner wall rotation is connected with screw (321), the upper end fixedly connected with handle (322) of screw (321), inner wall sliding connection (321) and screw (323).

2. An immune cell counting device according to claim 1, wherein: two movable grooves (22) are formed in the inner wall of the acquisition camera shooting barrel (2), a movable plate (221) is connected to the inner wall of the movable groove (22) in a sliding mode, the lower end of the movable plate (221) is fixedly connected with the zoom barrel (222), a rotating ring (21) is connected to the arc surface of the acquisition camera shooting barrel (2) in a rotating mode, a plurality of rings of dense thread grooves are formed in the inner wall of the rotating ring (21), and the outer wall of the movable plate (221) is meshed with the thread grooves in the inner wall of the rotating ring (21).

3. An immune cell counting device according to claim 1, wherein: one side of the sliding block (323) is fixedly connected with a limiting ring (324), the upper surface of the limiting ring (324) is fixedly connected with an anti-slip pad (325), and the anti-slip pad (325) is a rubber pad.

4. An immune cell counting device according to claim 1, wherein: scale marks (34) are formed in one side, close to the square groove (32), of the surface of the vertical plate (31), and the formed length of each scale mark (34) is equal to the length of the square groove (32).

5. An immune cell counting device according to claim 1, wherein: one side of the vertical plate (31) is fixedly connected with a marking plate (33).

6. An immune cell counting device according to claim 1, wherein: one side fixedly connected with auxiliary plate (4) of bottom plate (1), standing groove (41) have been seted up on the surface of auxiliary plate (4), a plurality of culture dish bodies (3) have been placed to the inner wall of standing groove (41).

7. An immune cell counting device according to claim 1, wherein: the upper surface of the workbench (11) is fixedly connected with a positioning ring (35) at the lower end position close to the acquisition camera cylinder (2), the positioning ring (35) is semi-circular, and the positioning ring (35) is a transparent ring.

8. An immune cell counting device according to claim 1, wherein: a plurality of illuminating lamps (12) are arranged on the inner wall of the workbench (11).