CN219824207U - Cell culture device for living cell imaging - Google Patents
Cell culture device for living cell imaging Download PDFInfo
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- CN219824207U CN219824207U CN202321120066.5U CN202321120066U CN219824207U CN 219824207 U CN219824207 U CN 219824207U CN 202321120066 U CN202321120066 U CN 202321120066U CN 219824207 U CN219824207 U CN 219824207U
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- fixedly connected
- shell
- cell culture
- cell
- culture dish
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- 238000004113 cell culture Methods 0.000 title claims abstract description 36
- 238000003384 imaging method Methods 0.000 title claims abstract description 25
- 239000000523 sample Substances 0.000 claims abstract description 15
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 18
- 239000001301 oxygen Substances 0.000 claims description 18
- 229910052760 oxygen Inorganic materials 0.000 claims description 18
- 239000007789 gas Substances 0.000 claims description 17
- 238000007789 sealing Methods 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 3
- 238000010859 live-cell imaging Methods 0.000 claims 7
- 230000009471 action Effects 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- 238000009434 installation Methods 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 8
- 230000008859 change Effects 0.000 description 5
- 238000005259 measurement Methods 0.000 description 4
- 239000000428 dust Substances 0.000 description 3
- 230000004103 aerobic respiration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture device for living cell imaging, which comprises a shell, wherein the upper surface of the shell is fixedly connected with a transparent plate, the middle part of the inner bottom wall of the shell is rotationally connected with a screw rod, the outer surface of the screw rod is in threaded connection with a sliding plate, and the upper surface of the sliding plate is provided with a groove; the inner wall of the groove is provided with a cavity. This a cell culture apparatus for living cell formation of image, through the setting of lead screw, the slide, the spring, splint and culture dish, it is fixed that the splint carries out the centre gripping to the culture dish under the elastic action of spring, the installation is dismantled conveniently, can make the culture dish more stable at the placing of device, rotate the lead screw, make the slide spiral rise, be convenient for adjust the position and the height of culture dish, be convenient for microscope probe is formed an image to the inside cell of each culture dish, make the cultivation and the integration setting of formation of image of cell, it is more accurate to measure the imaging data, the practicality of device has been increased.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a cell culture device for living cell imaging.
Background
Cell biology is the subject of research on cell-level life processes, and is described as a morphology in the early stage, after that, the cell absorbs molecular biology achievement and goes deep to the level of ultrastructure, so that biological processes such as cell growth, metabolism, genetics and the like are mainly researched, the cell biology is developed, and cell culture needs to rely on a cell culture device, so that a cell culture dish needs to be kept under proper conditions.
Through retrieving, chinese patent publication (bulletin) No. CN206916162U discloses a cell culture apparatus of adjustable gas concentration, through having set up spring, spring cylinder, depression bar, spacing ring and stopper, the gas concentration that makes cell culture apparatus possess can be adjusted, has effectually solved current cell culture apparatus because the cell carries out aerobic respiration, needs to consume the inside air of cell culture apparatus.
Although the above patent can adjust the gas concentration inside the device, it is inconvenient to image the cell measurement after the cell culture dish is placed inside the device, if the cell sample is separated from the device for microscopic imaging, the phenomenon that the measurement data is inaccurate is easily caused, and the use has limitations, so that it is necessary to design a cell culture device for living cell imaging to solve the above problems.
Disclosure of Invention
The utility model mainly aims to provide a cell culture device for living cell imaging, which can effectively solve the problems in the background technology.
In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:
the cell culture device for living cell imaging comprises a shell, wherein the upper surface of the shell is fixedly connected with a transparent plate, the middle part of the inner bottom wall of the shell is rotationally connected with a screw rod, the outer surface of the screw rod is in threaded connection with a sliding plate, and the upper surface of the sliding plate is provided with a groove; the inner wall of recess has seted up the cavity, the inside fixedly connected with spring of cavity, the one end fixedly connected with splint of spring, the surface swing joint of splint has the culture dish, the top fixedly connected with solid fixed ring of culture dish surface.
In order to achieve the effect of convenient display, as the cell culture device for living cell imaging, one side of the inner top wall of the transparent plate is fixedly connected with a microscope probe, and one side of the front surface of the shell is fixedly connected with a touch display screen.
In order to achieve the effect of being convenient for monitoring the change of the gas in the shell, as the cell culture device for living cell imaging, the other side of the inner top wall of the transparent plate is fixedly connected with a gas sensor.
In order to achieve the effect of convenient oxygenation, as the cell culture device for living cell imaging, one side of the inner bottom wall of the shell is fixedly connected with an oxygen generation pump, the inside of the oxygen generation pump is fixedly connected with a connecting pipe, and one end of the connecting pipe is fixedly connected with a filtering plug.
In order to achieve the effect of enhancing the sealing performance of the junction of the box door, as the cell culture device for living cell imaging, the box door is hinged to the other side of the front face of the shell through the hinge, and the sealing ring is fixedly connected to the back face of the box door.
In order to achieve the effect of being convenient for fixing the box door, as the cell culture device for living cell imaging, one sides of the front sides of the shell and the box door are provided with limiting grooves, and one side, close to the limiting grooves, of the inner part of the shell is rotationally connected with a stop block.
In order to achieve the effect of convenient operation, as the cell culture device for living cell imaging, rubber gloves are fixedly connected to two sides of the inner side surface of the shell.
In order to achieve the effect of being convenient for opening and closing the box door, as the cell culture device for living cell imaging, a handle is fixedly connected to the edge of the outer surface of the box door.
Compared with the prior art, the utility model has the following beneficial effects:
1. according to the utility model, through the arrangement of the screw rod, the sliding plate, the springs, the clamping plates and the culture dishes, the culture dishes are clamped and fixed by the clamping plates under the elastic action of the springs, the mounting and dismounting are convenient, the culture dishes can be placed in the device more stably, the screw rod is rotated, the sliding plate is spirally lifted, the position and the height of the culture dishes are convenient to adjust, the microscope probe is convenient to image cells in the culture dishes, the integrated arrangement of cell cultivation and imaging is realized, the measurement imaging data is more accurate, and the practicability of the device is improved.
2. According to the utility model, through the arrangement of the microscope probe, the touch display screen, the gas sensor and the oxygen generating pump, the microscope probe is in a vertical relation with the culture dish, when the culture dish moves to the upper part of the inside of the shell, cells in the culture dish can be imaged through the microscope probe and displayed through the touch display screen, so that the observation of workers is facilitated, the gas sensor monitors the change of the gas concentration in the inside of the shell, the oxygen generating pump transmits oxygen to the inside of the shell through the connecting pipe, the oxygen concentration in the inside of the device can be adjusted, and the problem that the cells stop growing due to the depletion of air consumption in the inside of the device is avoided.
Drawings
Fig. 1 is a schematic front view of embodiment 1 of the present utility model;
FIG. 2 is a schematic cross-sectional plan view of embodiment 1 of the present utility model;
fig. 3 is a schematic view showing the internal structure of a housing according to embodiment 1 of the present utility model;
FIG. 4 is a schematic view of a skateboard according to embodiment 1 of the present utility model;
fig. 5 is a schematic view showing the structure of a handle according to embodiment 2 of the present utility model.
In the figure: 1. a housing; 2. a transparent plate; 3. a screw rod; 4. a slide plate; 5. a groove; 6. a cavity; 7. a spring; 8. a clamping plate; 9. a culture dish; 10. a fixing ring; 11. a microscope probe; 12. touching the display screen; 13. a gas sensor; 14. an oxygen generating pump; 15. a connecting pipe; 16. filtering plugs; 17. a door; 18. a seal ring; 19. a limit groove; 20. a stop block; 21. rubber gloves; 22. a handle.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Example 1
1-4, a cell culture device for living cell imaging comprises a shell 1, wherein a transparent plate 2 is fixedly connected to the upper surface of the shell 1, a screw rod 3 is rotatably connected to the middle part of the inner bottom wall of the shell 1, a sliding plate 4 is connected to the outer surface of the screw rod 3 in a threaded manner, and a groove 5 is formed in the upper surface of the sliding plate 4;
in this embodiment, cavity 6 has been seted up to the inner wall of recess 5, and the inside fixedly connected with spring 7 of cavity 6, the one end fixedly connected with splint 8 of spring 7, the surface swing joint of splint 8 has culture dish 9, the top fixedly connected with solid fixed ring 10 of culture dish 9 surface.
When the device is specifically used, through the arrangement of the screw rod 3, the sliding plate 4, the groove 5, the spring 7, the clamping plate 8 and the culture dish 9, the transparent plate 2 is fixed on the upper surface of the shell 1, the operator can check the cultivation condition of cells inside the shell 1 from the outside, the culture dish 9 with living cells is placed inside the groove 5, the clamping plate 8 clamps and fixes the culture dish 9 under the elastic action of the spring 7, the device is convenient to mount and dismount, the culture dish 9 can be placed more stably in the device, the fixing ring 10 is convenient for take and place the culture dish 9, the hand force is convenient, the screw rod 3 is rotated, the sliding plate 4 is enabled to be spirally lifted, the position and the height of the culture dish 9 are convenient to adjust, the microscope probe 11 is convenient for imaging the cells inside the culture dish 9, the cultivation and the imaging integrated setting of the cells are convenient, the measurement imaging data are more accurate, and the practicability of the device is increased.
In this embodiment, a microscope probe 11 is fixedly connected to one side of the inner top wall of the transparent plate 2, and a touch display screen 12 is fixedly connected to one side of the front face of the housing 1.
When the device is specifically used, through the arrangement of the microscope probe 11 and the touch display screen 12, the microscope probe 11 is in vertical relation with the culture dish 9, when the culture dish 9 moves to the upper side inside the shell 1, cells inside the culture dish 9 can be imaged through the microscope probe 11, and the cells are displayed through the touch display screen 12, so that the device is convenient for workers to observe, and the practicability of the device is improved.
In this embodiment, the other side of the inner top wall of the transparent plate 2 is fixedly connected with a gas sensor 13.
When the cell culture device is particularly used, through the arrangement of the gas sensor 13, the gas sensor 13 can monitor the change of the gas concentration in the shell 1, and the problem that the change of the gas concentration in some cell culture devices cannot be observed is effectively solved.
In this embodiment, an oxygen generating pump 14 is fixedly connected to one side of the inner bottom wall of the housing 1, a connecting pipe 15 is fixedly connected to the inside of the oxygen generating pump 14, and a filtering plug 16 is fixedly connected to one end of the connecting pipe 15.
When the device is specifically used, through the arrangement of the oxygen making pump 14, the oxygen making pump 14 conveys oxygen to the inside of the shell 1 through the connecting pipe 15, so that the oxygen concentration in the device can be adjusted, the problem that cells stop growing due to the fact that air consumption in the device is completely consumed is avoided, and the air sucked by the connecting pipe 15 is filtered by the filtering plug 16, so that dust is prevented from entering.
In this embodiment, the other side of the front surface of the housing 1 is hinged with a door 17 through a hinge, and the back surface of the door 17 is fixedly connected with a sealing ring 18.
When the sealing device is specifically used, the box door 17 is hinged to the outer surface of the shell 1 through the hinge, so that the sealing device is convenient to open and close, and when the box door 17 is closed, the sealing ring 18 increases the tightness of the joint of the box door 17 and the inner side surface of the shell 1, so that external air and dust are prevented from entering, and the sealing effect is enhanced.
In this embodiment, the front sides of the housing 1 and the door 17 are both provided with a limit groove 19, and a stop block 20 is rotatably connected to the inner side of the housing 1 near the limit groove 19.
When the refrigerator door is specifically used, through the arrangement of the stop block 20, the stop block 20 is rotationally connected to the outer surface of the casing 1, close to the inside of the limit groove 19, and when the stop block 20 rotates to the inside of the limit groove 19 on the outer surface of the refrigerator door 17, the stop block 20 can be limited, so that the refrigerator door 17 is prevented from being opened at will.
In this embodiment, rubber gloves 21 are fixedly connected to both sides of the inner side of the housing 1.
When the device is specifically used, through the arrangement of the rubber glove 21, the rubber glove 21 is arranged in the shell 1, so that a worker can conveniently perform aseptic operation, and the culture dish 9 in the device can be observed in a refined mode, so that the operation of a closed aseptic environment is realized.
Working principle: in use, the box door 17 is opened, the culture dish 9 with living cells is placed in the groove 5, the clamp plate 8 clamps and fixes the culture dish 9 under the elastic action of the spring 7, the box door 17 is closed, the sealing ring 18 prevents external air and dust from entering, the stop block 20 is rotated, the box door 17 is fixed, the gas sensor 13 monitors the change of the gas concentration in the shell 1, the oxygen generating pump 14 conveys oxygen into the shell 1 through the connecting pipe 15, the oxygen concentration in the device can be adjusted, the rubber glove 21 is convenient for a worker to perform aseptic operation, the screw rod 3 is rotated, the slide plate 4 is spirally lifted, the position and the height of the culture dish 9 are adjusted, the microscope probe 11 is convenient for imaging the cells in each culture dish 9, and the integrated arrangement of cultivation and imaging of the cells is realized through the touch display screen 12, and the practicability of the device is improved.
Example 2
As shown in fig. 1 to 5, the distinguishing features of this embodiment that distinguish embodiment 1 are: a handle 22 is fixedly connected to the edge of the outer surface of the box door 17.
When the door 17 is particularly used, the handle 22 is convenient for a user to control the door 17 through the arrangement of the handle 22, so that the hand force is convenient, and the use convenience is improved.
The foregoing has shown and described the basic principles and main features of the present utility model and the advantages of the present utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (8)
1. A cell culture device for living cell imaging, comprising a housing (1), characterized in that: the upper surface of the shell (1) is fixedly connected with a transparent plate (2), the middle part of the inner bottom wall of the shell (1) is rotationally connected with a screw rod (3), the outer surface of the screw rod (3) is in threaded connection with a sliding plate (4), and the upper surface of the sliding plate (4) is provided with a groove (5);
the inner wall of recess (5) has seted up cavity (6), the inside fixedly connected with spring (7) of cavity (6), the one end fixedly connected with splint (8) of spring (7), the surface swing joint of splint (8) has culture dish (9), the top fixedly connected with solid fixed ring (10) of culture dish (9) surface.
2. A cell culture apparatus for live cell imaging according to claim 1, wherein: one side of the inner top wall of the transparent plate (2) is fixedly connected with a microscope probe (11), and one side of the front surface of the shell (1) is fixedly connected with a touch display screen (12).
3. A cell culture apparatus for live cell imaging according to claim 1, wherein: the other side of the inner top wall of the transparent plate (2) is fixedly connected with a gas sensor (13).
4. A cell culture apparatus for live cell imaging according to claim 1, wherein: one side of the inner bottom wall of the shell (1) is fixedly connected with an oxygen generating pump (14), the inside of the oxygen generating pump (14) is fixedly connected with a connecting pipe (15), and one end of the connecting pipe (15) is fixedly connected with a filtering plug (16).
5. A cell culture apparatus for live cell imaging according to claim 1, wherein: the other side of the front face of the shell (1) is hinged with a box door (17) through a hinge, and the back face of the box door (17) is fixedly connected with a sealing ring (18).
6. A cell culture apparatus for live cell imaging according to claim 5, wherein: limiting grooves (19) are formed in one sides of the front faces of the shell (1) and the box door (17), and a stop block (20) is rotatably connected to one side, close to the limiting grooves (19), of the interior of the shell (1).
7. A cell culture apparatus for live cell imaging according to claim 1, wherein: rubber gloves (21) are fixedly connected to two sides of the inner side face of the shell (1).
8. A cell culture apparatus for live cell imaging according to claim 5, wherein: the edge of the outer surface of the box door (17) is fixedly connected with a handle (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321120066.5U CN219824207U (en) | 2023-05-11 | 2023-05-11 | Cell culture device for living cell imaging |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321120066.5U CN219824207U (en) | 2023-05-11 | 2023-05-11 | Cell culture device for living cell imaging |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219824207U true CN219824207U (en) | 2023-10-13 |
Family
ID=88246675
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321120066.5U Active CN219824207U (en) | 2023-05-11 | 2023-05-11 | Cell culture device for living cell imaging |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219824207U (en) |
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2023
- 2023-05-11 CN CN202321120066.5U patent/CN219824207U/en active Active
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