CN219777700U - Reusable test fixture - Google Patents
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- CN219777700U CN219777700U CN202320288914.7U CN202320288914U CN219777700U CN 219777700 U CN219777700 U CN 219777700U CN 202320288914 U CN202320288914 U CN 202320288914U CN 219777700 U CN219777700 U CN 219777700U
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- 238000005538 encapsulation Methods 0.000 claims abstract description 15
- 238000004806 packaging method and process Methods 0.000 claims abstract description 14
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 238000011056 performance test Methods 0.000 claims abstract description 4
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a reusable test fixture, and belongs to the technical field of biochemical instruments. The test fixture is used for performance test of biochemical instruments, and comprises: the test device comprises a test substrate, wherein a plurality of detection sites are formed on the test substrate; each detection site is filled with a biochemical sample, and a solid transparent packaging layer for sealing the biochemical sample in the detection site; the biochemical sample is dispersed in the solid transparent encapsulation layer. The test fixture seals the biochemical sample in the detection site through the solid transparent packaging layer, so that the biochemical sample is prevented from being polluted and is convenient for long-term storage, and the biochemical sample can be kept in a fixed position in the transfer process, thereby achieving good test reproducibility, and therefore, the test fixture can be reused.
Description
Technical Field
The utility model belongs to the technical field of biochemical instruments, and particularly relates to a reusable test fixture.
Background
Before a biochemical instrument is used for testing a biochemical sample, a testing tool is required to be adopted to verify whether the performance of the biochemical instrument meets the testing capability or metering requirement of instrument equipment. For example, in the protein joint inspection project, a magnetic bead counting standard plate is required to be adopted to debug a detection module of the protein joint inspection integrated machine and verify the delivery function; in the gene sequencing project, the optical system of the sequencing standard chip auxiliary gene sequencer is also required to be adopted to realize the leveling of the chip platform of the gene sequencer, and the like.
At present, the magnetic bead counting standard plate is generally prepared by the following method: diluting the magnetic mother-of-pearl liquid with an organic solution to obtain a magnetic mother-of-pearl liquid diluent, uniformly mixing the magnetic mother-of-pearl liquid diluent with a vortex instrument, taking quantitative magnetic mother-of-pearl liquid diluent with a liquid transfer device, adding the quantitative magnetic mother-of-pearl liquid diluent into a detection site of a cell counting plate, covering a cover glass, and finally obtaining the magnetic bead counting standard plate. Similarly, a sequencing standard chip is generally prepared by the following method: diluting a gene sample by using an organic solution to obtain a gene sample diluent, uniformly mixing the gene sample diluent by using a vortex instrument, measuring and taking quantitative gene sample diluent by using a liquid transfer device, adding the quantitative gene sample diluent into a detection site of a sequencing chip, covering a cover glass, and finally obtaining the sequencing standard chip.
The test tools in the related art are all obtained by adopting the existing manufacturing method, and the reason is that: (1) The biochemical sample in the detection site of the test tool is generally a solution system, the detection site is only covered by a cover slip, so that the situation that external microorganisms fall into growth and propagation and dust fall into the biochemical sample to pollute the biochemical sample easily cannot be avoided, and the solution is easy to volatilize, so that the test tool cannot be stored for a long time, and therefore, the test tool cannot be reused and can only be used in the process of production; (2) The biochemical sample in the detection site of the test tool is generally a solution system, so that along with the transfer position and movement of the test tool, the position of the internal biochemical sample can be changed, so that the biochemical sample is overlapped, and the test tool can not be reused, so that the test tool can only be used in actual production.
However, the existing test tool has the following obvious problems: (1) The biochemical samples in the test tool are easy to be polluted, so that the biochemical samples cannot be stored for a long time, and the test tool cannot be reused; (2) The biochemical sample in the test tool is generally a solution system, so that the position and movement are inconvenient to transfer; (3) The test fixture needs to be manufactured at present each time, but in the preparation process of different batches, the added biochemical sample amount has randomness, so that the repeatability of the test fixture manufactured in different batches is easy to be poor; (4) The test fixture needs to be manufactured at present every time, so that a great deal of effort is required in the manufacturing process, and the manufacturing cost of the test fixture is high.
Therefore, the research on the test tool for testing the performance of the biochemical instrument in the related art is insufficient, and further improvement is needed, so that the reusable test tool is designed.
Disclosure of Invention
The present utility model has been made based on the findings and knowledge of the inventors regarding the following facts and problems: the test fixture in the related art is difficult to reuse and can only be used in the present process, and the time and the cost are high, so that further improvement is needed, and a reusable test fixture is designed.
The present utility model aims to solve at least one of the technical problems in the related art to some extent. Therefore, the embodiment of the utility model provides a reusable test fixture. The test tool provided by the embodiment of the utility model can be repeatedly used without existing production and use, so that the time and the cost for duplicating samples each time can be saved, and the use cost is reduced.
The reusable test fixture of the embodiment of the utility model is used for performance test of biochemical instruments and comprises the following components:
the test device comprises a test substrate, wherein a plurality of detection sites are formed on the test substrate;
each detection site is filled with a biochemical sample, and a solid transparent packaging layer for sealing the biochemical sample in the detection site; the biochemical sample is dispersed in the solid transparent encapsulation layer.
The reusable test fixture provided by the embodiment of the utility model has the following advantages and technical effects:
(1) Avoiding pollution, and can be stored for a long time:
according to the test tool provided by the embodiment of the utility model, the solid system is adopted in the detection site, so that the situation that the biochemical sample is polluted due to microorganism breeding and dust entering is thoroughly avoided, and if the surface of the test tool is polluted by dust, stains, microorganisms and the like, the test tool can be directly cleaned by dipping alcohol with the lens wiping paper, and the anti-pollution capability of the test tool is effectively enhanced, so that the test tool can realize the purpose of long-term storage;
(2) The biochemical sample is stable, and the test fixture is convenient for shift the position, and reproducibility is good:
according to the test tool disclosed by the embodiment of the utility model, after the biochemical samples in the detection sites are fixed by the solid transparent packaging layer, the biochemical samples lose the motion capability, the positions of the biochemical samples are not changed any more, and the quantity and the positions of the biochemical samples in the detection sites are constant, so that the test tool can conveniently transfer the positions and the reproducibility is not influenced;
(3) Can be repeatedly used:
the test tool provided by the embodiment of the utility model can be stored for a long time, and the test reproducibility is not affected after the position is transferred, so that the test tool can be reused for multiple times;
(4) Reducing time and cost loss of duplicate samples
The reusable test tool provided by the embodiment of the utility model can be taken at any time without being used in the prior art, so that the reusable test tool can be conveniently used for testing or metering the capability of instruments and equipment, the preparation work of the test tool before each use is avoided, and the time and the cost are greatly saved.
In some embodiments, the biochemical sample comprises a magnetic bead sample or a gene sample.
In some embodiments, the solid transparent encapsulation layer is a cured UV glue layer or a UV epoxy layer.
In some embodiments, the detection sites are grooves recessed in a thickness direction of the test substrate.
In some embodiments, the top highest point of the solid transparent encapsulation layer is not higher than the top surface of the detection site.
In some embodiments, the top highest point of the solid transparent encapsulation layer is flush with the top surface of the detection site.
In some embodiments, the test substrate includes a frame layer and a transparent bottom plate covering one side of the frame layer along a thickness direction of the frame layer, a through hole is formed in the frame layer in a penetrating manner along the thickness direction, and the transparent bottom plate seals one end of the through hole and encloses the detection site with the through hole.
In some embodiments, the plurality of through holes are arranged in an array.
In some embodiments, the test substrate is a 96-well plate.
Drawings
FIG. 1 is a schematic perspective view of a reusable magnetic bead counting standard plate according to example 1 of the present utility model;
FIG. 2 is a schematic top view of the reusable magnetic bead counting standard plate of example 1 of the present utility model;
FIG. 3 is a schematic A-A section structure of a reusable magnetic bead count standard plate of example 1 of the present utility model;
FIG. 4 is a schematic diagram showing the perspective structure of a reusable sequencing standard chip according to embodiment 2 of the present utility model;
reference numerals illustrate: 1-testing a substrate; 2-detection sites; 3-biochemical samples; 4-a solid transparent encapsulation layer; 11-a frame layer; 12-a transparent base plate; 13-through holes.
Detailed Description
Reference will now be made in detail to embodiments of the present utility model, examples of which are illustrated in the accompanying drawings. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model.
As shown in fig. 1-3, the reusable test fixture according to the embodiment of the present utility model is used for performance test of biochemical instruments, and includes:
the test substrate 1, a plurality of detection sites 2 are arranged on the test substrate 1;
each detection site 2 is filled with a biochemical sample 3 and a solid transparent packaging layer 4 for sealing the biochemical sample 3 in the detection site 2; the biochemical sample 3 is dispersed in the solid transparent encapsulation layer 4.
Working principle: according to the test tool disclosed by the embodiment of the utility model, the position of the biochemical sample is fixed in the detection site 2 by adopting the solid transparent packaging layer 4, so that a solid system is formed, and the pollution of the biochemical sample 3 after microorganisms and dust enter the detection site 2 is avoided, so that the test tool can be stored for a long time. When the surface of the test tool is polluted by dust, stains, microorganisms and the like, the test tool can be directly cleaned by dipping alcohol with the mirror wiping paper, so that the anti-pollution capability of the test tool is effectively enhanced. In addition, after the biochemical samples 3 in the detection sites 2 are fixed by the solid transparent packaging layer 4, the movement capability is lost, and the positions are not changed any more, so that the quantity and the positions of the biochemical samples 3 in each detection site 2 are constant and consistent, the position of the test tool can be randomly transferred, and the repeatability is good when the test tool is used for detecting after the transfer. In addition, compared with the existing test tools in the related art, the test tool can be taken at any time, so that the test tool can be conveniently used for testing the performance of instruments and equipment, the preparation work of the test tool before each use is omitted, the time is greatly saved, the cost of duplicating samples each time is saved, and the purpose of reducing the use cost is realized.
It can be understood that the test tool of the embodiment of the utility model is used for verifying the performance of the instrument when the biochemical instrument is tested in batch, and has no special requirements on the activity and the number of the biochemical samples 3, so long as the corresponding biochemical samples 3 are fixed in the detection sites 2, and the packaging layer for fixing and sealing the biochemical samples 3 is transparent, and the observation is not affected.
In some embodiments, the biochemical sample 3 comprises a magnetic bead sample or a gene sample. Specifically, when the test tool is a magnetic bead counting standard plate, the biochemical sample 3 is a magnetic bead sample; when the test tool is a sequencing standard chip, the biochemical sample 3 is a gene sample.
In some embodiments, the solid transparent encapsulation layer 4 is a cured UV glue layer or UV epoxy layer. UV glue, also known as shadowless glue, photosensitive glue or UV curable glue, is a type of adhesive that must be cured by UV light irradiation. The cured UV adhesive layer has high transparency and meets the detection requirement of the test tool of the embodiment of the utility model. The UV epoxy resin belongs to photosensitive resin, and is an epoxy system oligomer which can rapidly generate physical and chemical changes in a short time after being irradiated by ultraviolet light so as to crosslink and cure. The cured UV epoxy resin layer is high in transparency, and meets the detection requirements of the test tool of the embodiment of the utility model.
The embodiment of the utility model has no special requirement on the shape of the detection site 2, and can only accommodate the biochemical sample 3 and the solid transparent packaging layer 4 without influencing the observation. For example, the cross-section of the detection sites 2 may be circular, oval, square, rectangular, triangular, polygonal, etc.
As shown in fig. 4, in some embodiments, the detection sites 2 are grooves recessed in the thickness direction of the test substrate 1. The diameter of the grooves can be uniform from top to bottom, or gradually reduced from top to bottom, or gradually increased from top to bottom.
In some embodiments, the highest point of the top of the solid transparent encapsulation layer 4 is not higher than the top surface of the detection site 2, which is convenient for use.
In some embodiments, the top highest point of the solid transparent encapsulation layer 4 is flush with the top surface of the detection site 2. The solid transparent packaging layer 4 can fill the detection site 2 to the greatest extent, prevent dust, microorganisms and the like from accumulating in the detection site 2, and clean the detection site 2 more easily.
In some embodiments, the test substrate 1 includes a frame layer 11 and a transparent bottom plate 12 covering one side of the frame layer 11 along a thickness direction of the frame layer 11, and a through hole 13 is disposed on the frame layer 11 in a penetrating manner along the thickness direction, and the transparent bottom plate 12 seals one end of the through hole 13 and encloses the detection site 2 with the through hole 13. The transparent base plate 12 facilitates observation during testing.
In some embodiments, the through holes 13 are plural and arranged in an array.
In some embodiments, the test substrate 1 comprises a 96-well plate or a 48-well plate, or the like.
The material of the test substrate needs to have chemical stability, and is not dissolved by UV glue or UV epoxy resin and a diluent used in the preparation method described below, and is not easy to change color after being irradiated by ultraviolet light.
The embodiment of the utility model also provides a preparation method of the reusable test fixture, which comprises the following steps:
(1) Uniformly mixing a biochemical sample and a diluent to obtain a biochemical sample diluent, and adding the biochemical sample diluent into the detection sites 2 of the clean test substrate 1;
(2) Placing the test substrate 1 added with the biochemical sample diluent in the step (1) on a temperature-controlled oscillator for running, so that the biochemical sample is uniformly dispersed in the detection sites 2;
(3) Heating the test substrate 1 oscillated in the step (2), and drying the diluent;
(4) Dripping UV glue or UV epoxy resin into the detection sites 2 of the dried test substrate 1 in the step (3);
(5) Vacuumizing the test substrate 1 with the UV glue or the UV epoxy resin in the step (4) by using vacuum equipment;
(6) And (3) carrying out photo-curing on the UV glue or the UV epoxy resin in the test substrate 1 subjected to the vacuumizing in the step (5) to form a solid transparent packaging layer 4, and finally obtaining the reusable test fixture.
Working principle: according to the preparation method provided by the embodiment of the utility model, the prepared test tool is a solid system, so that microorganism breeding can be stopped, the influence of dust and impurities is prevented, and the purpose of long-term storage is realized; moreover, the prepared test tool can be wiped, and has strong pollution resistance; in addition, the preparation method of the embodiment of the utility model can ensure the stability of the quantity and the position of the biochemical samples 3 in the detection sites 2, and the obtained test fixture is convenient to repeatedly use; in addition, the test tool is obtained by using the preparation method of the embodiment of the utility model, so that the sample can be taken at any time and used at any time, the anti-copy sample time is saved, and the anti-copy sample cost is also saved; in addition, according to the preparation method provided by the embodiment of the utility model, the step (1) and the step (2) can ensure that the biochemical sample 3 is uniformly dispersed in a curing system in the detection site 2, so that aggregation and stacking phenomena are effectively reduced, and observation use is prevented from being influenced; in addition, according to the preparation method provided by the embodiment of the utility model, in the step (5), gaps and bubbles between the UV glue or the UV epoxy resin and the detection site 2 can be removed, so that the observation use is prevented from being influenced; in addition, the preparation method provided by the embodiment of the utility model has the advantages of simple preparation process, convenience in operation and contribution to industrial popularization.
In some embodiments, in the step (1), the biochemical sample 3 and the diluent are mixed uniformly by using a vortex apparatus or the like to obtain a biochemical sample diluent, and a quantitative biochemical sample diluent is measured by using a pipette and added into the detection site 2 of the clean test substrate 1.
In some embodiments, in step (4), the UV glue or UV epoxy has a viscosity of 500-2000mPas. The UV gel or UV epoxy resin within the above viscosity range is more advantageous for uniformly dispersing the biochemical samples 3. If the viscosity of the UV gel or UV epoxy is too low, it may cause a decrease in transparency after curing, which is disadvantageous for observation. If the viscosity of the UV glue or UV epoxy resin is too high, the biochemical samples 3 are not uniformly dispersed in the solid transparent encapsulation layer 4, aggregation and stacking phenomena may occur, and further the observation and the use are not facilitated.
In some embodiments, after the UV glue or UV epoxy is drip-coated in step (4), the level of the UV glue or UV epoxy is made flush with the highest point of the detection site 2. After the UV glue or UV epoxy resin is cured, as shown in fig. 2, the surface of the UV glue or UV epoxy resin is recessed downwards, so that the UV glue layer or UV epoxy resin layer can be filled in the detection site 2 to the greatest extent, dust, microorganisms and the like are prevented from accumulating in the detection site 2, and the detection site 2 is easier to clean.
The present utility model will be described in detail with reference to the following examples and drawings.
Example 1
As shown in fig. 1-3, the reusable magnetic bead counting standard plate of the present embodiment includes a 96-well elisa plate (test substrate 1), the 96-well elisa plate (test substrate 1) includes a frame layer 11 and a transparent bottom plate 12 covering one side of the frame layer 11 along the thickness direction of the frame layer 11, a plurality of through holes 13 arranged in an array are arranged on the frame layer 11 in a penetrating manner along the thickness direction, and the transparent bottom plate 12 seals one end of the plurality of through holes 13 and encloses a detection site 2 with the plurality of through holes 13; each detection site 2 is filled with magnetic beads (biochemical samples 3) and a cured UV adhesive layer (solid transparent packaging layer 4) for sealing the magnetic bead samples (biochemical samples 3) in the detection sites 2; the magnetic bead samples (biochemical samples 3) are uniformly dispersed in the UV glue layer (solid transparent packaging layer 4), and the number of the magnetic beads (biochemical samples 3) in each detection site 2 is 50.
The reusable magnetic bead counting standard plate of the embodiment can be manufactured by the following steps:
(1) Mixing the magnetic bead mother solution and an organic solvent (diluent), uniformly mixing by using a vortex instrument to obtain a magnetic bead diluent, metering a certain amount of the magnetic bead diluent by using a pipettor, and adding the magnetic bead diluent into a through hole 13 of a clean 96-hole ELISA plate;
(2) The 96-hole ELISA plate added with the magnetic bead diluent is arranged on a temperature control oscillator to run, so that the magnetic bead sample is ensured to be uniformly dispersed in the detection site 2, and aggregation and stacking phenomena are reduced;
(3) Heating the 96-hole ELISA plate with the magnetic bead diluent at 85 ℃ and drying and removing the organic solvent (diluent);
(4) Drip-irrigating low-viscosity UV glue into the detection site 2 of the dried 96-hole ELISA plate, ensuring that the liquid level is flush with the top surface of the detection site 2, and the viscosity of the UV glue is 1000mPas;
(5) Vacuumizing the 96-hole ELISA plate for drip irrigation of the UV glue by using vacuum equipment, and removing gaps and bubbles between the UV glue and the detection sites 2 to prevent the influence on observation and use;
(6) And taking out the vacuumized 96-hole ELISA plate, and curing by using a UV lamp to obtain the reusable magnetic bead counting standard plate.
Evaluation of performance: in the protein joint inspection project, the reusable magnetic bead counting standard plate is used for debugging and verifying the delivery function of a detection module of the protein joint inspection integrated machine. The evaluation results show that: the magnetic bead counting standard plate has good quality, can meet the requirements of the protein joint inspection integrated machine on capability inspection or metering, can realize 100% reproducibility in the debugging process of different protein joint inspection integrated machines or the use process of the same protein joint inspection integrated machine in different time periods, and can lead the capability inspection or metering work of the protein joint inspection integrated machine to be standardized.
The reusable magnetic bead counting standard plate is used for detection, and the following purposes can be achieved:
(1) Convenient: the sample can be taken at any time, so that the sample preparation time is saved;
(2) The method can be repeated: standardized products can be repeatedly used, and the result is reproducible;
(3) Low cost: the material cost and the time cost of the anti-corrosion sample preparation are saved;
(4) Easy storage: can be stored in normal room temperature environment without being affected by bacteria, dust impurities and vapor evaporation.
Example 2
As shown in fig. 4, the reusable sequencing standard chip of the embodiment includes a test substrate 1, wherein a plurality of detection sites 2 arranged in an array are arranged on the test substrate 1 along the thickness direction, the detection sites 2 are grooves formed by recessing the surface of the test substrate 1, and each detection site 2 is filled with a DNA sample and a cured UV glue layer sealing the DNA sample in the detection site 2; the DNA samples were uniformly dispersed within the UV gel layer.
Evaluation of performance: in the gene sequencing project, the reusable sequencing standard chip is used for leveling a chip platform of a gene sequencer. The evaluation results show that: the sequencing standard chip has good quality, can meet the leveling requirement of a chip platform of a gene sequencer, can realize 100% reproducibility in the leveling process of different gene sequencers or the use process of the same gene sequencer in different time periods, and can standardize the leveling work of the chip platform.
The multiplexing sequencing standard chip is used for detection, so that the following purposes can be achieved:
(1) Convenient: the sample can be taken at any time, so that the sample preparation time is saved;
(2) The method can be repeated: standardized products can be repeatedly used, and the result is reproducible;
(3) Low cost: the material cost and the time cost of the anti-corrosion sample preparation are saved;
(4) Easy storage: can be stored in normal room temperature environment without being affected by bacteria, dust impurities and vapor evaporation.
For purposes of this disclosure, the terms "one embodiment," "some embodiments," "example," "a particular example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
While embodiments of the present utility model have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the utility model, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the utility model.
Claims (8)
1. The utility model provides a can multiplexing test fixture for biochemical instrumentation's performance test, its characterized in that includes:
the test device comprises a test substrate, wherein a plurality of detection sites are formed on the test substrate;
each detection site is filled with a biochemical sample, and a solid transparent packaging layer for sealing the biochemical sample in the detection site; the biochemical sample is dispersed in the solid transparent encapsulation layer.
2. The reusable test fixture of claim 1, wherein the biochemical samples comprise magnetic bead samples or gene samples.
3. The reusable test fixture of claim 1, wherein the solid transparent encapsulation layer is a cured UV glue layer or UV epoxy layer.
4. The reusable test fixture of claim 1, wherein a top highest point of the solid transparent encapsulation layer is not higher than a top surface of the detection site.
5. The reusable test fixture of claim 4, wherein a top highest point of the solid transparent encapsulation layer is flush with a top surface of the detection site.
6. The reusable test fixture according to any one of claims 1-5, wherein the test substrate comprises a frame layer and a transparent bottom plate covering one side of the frame layer along a thickness direction of the frame layer, a through hole is formed in the frame layer in a penetrating manner along the thickness direction, and the transparent bottom plate seals one end of the through hole and encloses the detection site with the through hole.
7. The reusable test fixture of claim 6, wherein the plurality of through holes are arranged in an array.
8. The reusable test fixture of claim 7, wherein the test substrate is a 96-well plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320288914.7U CN219777700U (en) | 2023-02-08 | 2023-02-08 | Reusable test fixture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320288914.7U CN219777700U (en) | 2023-02-08 | 2023-02-08 | Reusable test fixture |
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| Publication Number | Publication Date |
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| CN219777700U true CN219777700U (en) | 2023-09-29 |
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| CN202320288914.7U Active CN219777700U (en) | 2023-02-08 | 2023-02-08 | Reusable test fixture |
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| CN (1) | CN219777700U (en) |
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