CN219709478U - Cartridge for molecular detection - Google Patents
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- CN219709478U CN219709478U CN202320114218.4U CN202320114218U CN219709478U CN 219709478 U CN219709478 U CN 219709478U CN 202320114218 U CN202320114218 U CN 202320114218U CN 219709478 U CN219709478 U CN 219709478U
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- 238000001514 detection method Methods 0.000 title claims abstract description 86
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 46
- 230000009471 action Effects 0.000 claims abstract description 12
- 238000001125 extrusion Methods 0.000 claims abstract description 6
- 239000012528 membrane Substances 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims description 68
- 150000007523 nucleic acids Chemical class 0.000 claims description 55
- 102000039446 nucleic acids Human genes 0.000 claims description 51
- 108020004707 nucleic acids Proteins 0.000 claims description 51
- 239000000243 solution Substances 0.000 claims description 41
- 238000006243 chemical reaction Methods 0.000 claims description 40
- 102000004190 Enzymes Human genes 0.000 claims description 39
- 108090000790 Enzymes Proteins 0.000 claims description 39
- 239000011324 bead Substances 0.000 claims description 36
- 238000005406 washing Methods 0.000 claims description 30
- 230000009089 cytolysis Effects 0.000 claims description 23
- 238000004891 communication Methods 0.000 claims description 21
- 230000003321 amplification Effects 0.000 claims description 20
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 20
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 20
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 20
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims description 20
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims description 20
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 20
- 238000000605 extraction Methods 0.000 claims description 17
- 239000011259 mixed solution Substances 0.000 claims description 10
- 239000006166 lysate Substances 0.000 claims description 9
- 238000003776 cleavage reaction Methods 0.000 claims description 6
- 230000007017 scission Effects 0.000 claims description 6
- 238000012123 point-of-care testing Methods 0.000 abstract description 6
- 238000005336 cracking Methods 0.000 description 16
- 239000000126 substance Substances 0.000 description 12
- 230000009286 beneficial effect Effects 0.000 description 11
- 102000004169 proteins and genes Human genes 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 239000012634 fragment Substances 0.000 description 6
- 230000006037 cell lysis Effects 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000002934 lysing effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000001516 effect on protein Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to the field of molecular detection equipment, and particularly relates to a cartridge for molecular detection. The kit is provided with a reagent vesicle, one side of the reagent vesicle facing the inside of the kit body is provided with an extrusion rupture membrane, and the extrusion rupture membrane ruptures under the action of external force to release the reagent in the reagent vesicle during detection. When the detection is needed, the reagent vesicle can release the internal reagent under the action of external force so as to finish the sample treatment, and the reagent vesicle as a reagent accommodating structure is well sealed with the whole cartridge, so that the detection cost is reduced, and meanwhile, when the detection is needed, the reagent cartridge is only required to be put in a proper position in the detector, the detection step is simple, and the detection time is shortened, thereby facilitating the family of the molecular POCT detection.
Description
Technical Field
The utility model belongs to the field of molecular detection equipment, and particularly relates to a cartridge for molecular detection.
Background
Molecular detection refers to a technique for detecting the structural or expression level change of genetic material in a patient side body by using a molecular biological method, and the molecular detection mainly comprises three steps, namely nucleic acid extraction, nucleic acid amplification and detection. During detection, firstly, nucleic acid in a collected sample is extracted, and then the collected nucleic acid is amplified to increase the quantity of extracted genetic materials so as to facilitate the subsequent more accurate detection. Molecular detection POCT can be performed on site by means of POCT detection technology and analyzed immediately, and has the comprehensive advantages of rapidness, convenience and low cost, and can be popularized to realize the family of molecular detection.
In the prior art, a cartridge for molecular detection is usually prepared by injecting a lysis solution, a cleaning solution and a reactive enzyme into a sample respectively by using a piston extraction or other pneumatic structures, disrupting and lysing biological cells collected by the lysis solution to release genetic materials such as nucleic acid, removing impurities such as cell fragments and proteins in the mixed solution by using the cleaning solution, amplifying the genetic materials such as nucleic acid by entering an amplification region, and performing subsequent detection. The detection device in the prior art has a complex structure, the detection instrument executes a plurality of steps, the detection cost is increased, the detection time is too long, special equipment is needed to be used in a special detection room, and the detection device is not beneficial to the families of molecular detection POCT.
Disclosure of Invention
The utility model aims to provide a cartridge for molecular detection, which solves the technical problems of high molecular POCT detection cost, complex detection steps and long detection time consumption in the prior art.
In order to achieve the above purpose, the technical scheme of the cartridge for molecular detection provided by the utility model is as follows: the kit is provided with a reagent vesicle, one side of the reagent vesicle facing the inside of the kit body is provided with an extrusion rupture membrane, and the extrusion rupture membrane is ruptured under the action of external force to release the reagent in the reagent vesicle during detection.
The beneficial effects are that: when the detection is needed, the reagent vesicle can release the internal reagent under the action of external force so as to finish the sample treatment, and the reagent vesicle as a reagent accommodating structure is well sealed with the whole cartridge, so that the detection cost is reduced, and meanwhile, when the detection is needed, the reagent cartridge is only required to be put in a proper position in the detector, the detection step is simple, and the detection time is shortened, thereby facilitating the family of the molecular POCT detection.
Further, the cartridge comprises a nucleic acid extraction part, a communication channel and a cleaning area which are connected with the nucleic acid amplification part are arranged on the nucleic acid extraction part, and a valve is arranged on the communication channel; the reagent vesicles are provided on the nucleic acid extraction unit.
The beneficial effects are that: the nucleic acid extraction part and the nucleic acid amplification part are arranged on the cartridge, and when the nucleic acid detection device is used, the nucleic acid extraction and the amplification of the sample can be completed only by injecting the sample into the cartridge, so that the detection steps are simplified.
Further, a cracking area is arranged on the nucleic acid extraction part, the cracking area is communicated with a cleaning area, and the communicating channel is arranged on the cleaning area; the reagent vesicles on the lysis zone are lysis solution vesicles for containing lysis solution, and the reagent vesicles on the cleaning zone are cleaning solution vesicles for containing cleaning solution.
The beneficial effects are that: according to the process of extracting nucleic acid, the nucleic acid extracting part is partitioned, and different reagent vesicles are correspondingly arranged on different areas, when the sample is detected, the sample is treated by using a lysate to crack sample cells, substances inside the sample cells are released, and the sample cells are cleaned by using a cleaning liquid to remove proteins, cell fragments and other substances except nucleic acid generated after the cell cracking, so that only the nucleic acid is ensured to enter the amplifying part, the extraction of the nucleic acid is finished, and the smooth amplification of the nucleic acid is ensured.
Further, the cleaning area is also provided with a reagent vesicle for accommodating the reaction enzyme, the reagent vesicle for accommodating the reaction enzyme is a reaction enzyme vesicle, the reaction enzyme is not mutually dissolved with the cleaning solution, and the lysate is not mutually dissolved with the reaction enzyme and the cleaning solution.
The beneficial effects are that: the kit has the advantages that the reaction enzyme is also filled into the vesicles without arranging a reaction area, the mechanism of the kit is simplified, the reaction enzyme and the cleaning solution are not mutually dissolved, when the reaction enzyme vesicles and the cleaning solution vesicles release internal reagents simultaneously, the reagents are not mutually dissolved, when detection is carried out, the lysis solution vesicles, the cleaning solution vesicles and the reaction enzyme vesicles release corresponding reagents, the reagents can be naturally layered after release, a valve is not required to be arranged between the cleaning area and the lysis area, the structure of the kit is simplified, the detection step is simplified, the detection efficiency is improved, the detection cost is reduced, the cleaning effect on proteins, cell fragments and other substances generated after cell lysis can be ensured, and the reaction enzyme and the nucleic acid enter into a communication channel for the next nucleic acid amplification.
Further, the splitting area is also provided with a reagent vesicle for containing the nano magnetic beads, and the reagent vesicle for containing the nano magnetic beads is a magnetic bead vesicle.
The beneficial effects are that: the nanometer magnetic beads can be coupled with substances such as proteins and nucleic acids after cell lysis, the movement of the nucleic acid substances is realized through the magnetic field change, the nucleic acid substances can be ensured to accurately move to each area in the card box, and meanwhile, the nanometer magnetic beads are filled into vesicles, so that the nanometer magnetic beads can be conveniently injected into a lysis area and combined with various substances after the lysis.
Further, the reagent vesicles are spherical vesicles.
The beneficial effects are that: the spherical vesicle can be ruptured by only pressing the highest point of the arc-shaped part, so that the reagent inside the reagent vesicle can be released conveniently.
Further, the lysis solution vesicles, the magnetic bead vesicles and the lysis zone are concentrically arranged, and the lysis solution vesicles and the magnetic bead vesicles are respectively protruded out of the top surface and the bottom surface of the cartridge; the cleaning solution vesicles and the reactive enzyme vesicles are concentrically arranged and respectively protrude out of the top surface and the bottom surface of the cartridge.
The beneficial effects are that: the positions of the cleavage vesicles, the magnetic bead vesicles and the cleaning solution vesicles and the reaction enzyme vesicles are reasonably arranged, so that the positions of the vesicles on the whole cartridge are reasonably arranged, the size of the cartridge is convenient to reduce, and the cartridge is more convenient to transport and carry.
Further, two cleaning areas are arranged along the flowing direction of the sample, namely a first cleaning area and a second cleaning area, wherein a first cleaning fluid vesicle and a second cleaning fluid vesicle are respectively arranged on the first cleaning area and the second cleaning area, and a reactive enzyme vesicle is arranged on the second cleaning area.
The beneficial effects are that: the provision of two washing zones and two washing fluid vesicles ensures better removal of proteins and cell debris generated after cell lysis.
Further, the nucleic acid amplification part comprises at least two mixed solution reaction areas, and the primer types in any two mixed solution reaction areas are different; the communication channel between the cleaning area and the nucleic acid amplification part is provided with a plurality of sample outlets which are respectively communicated with the corresponding mixed liquid reaction areas.
The beneficial effects are that: different kinds of primers are conveniently added into different mixed liquid reaction areas to amplify different nucleic acid fragments, so that the efficiency of molecular detection is improved.
Further, the valve on the communication channel is a wax valve or a unidirectional PDMS soft valve, and when the valve on the communication channel is a wax valve, the cartridge needs to be heated to open the wax valve.
The beneficial effects are that: the wax valve and the unidirectional PDMS soft valve are simple in structure, the cost of the cartridge is reduced, and the unidirectional PDMS soft valve can also prevent the mixed liquid entering the cartridge for molecular detection from flowing back into the cracking zone.
Drawings
FIG. 1 is a plan view of a cartridge for molecular detection in example 1 of the present utility model;
FIG. 2 is a side view of the cartridge for molecular detection in example 1;
FIG. 3 is a block diagram of a one-way PDMS soft valve of the cartridge for molecular assay in example 1;
FIG. 4 is a schematic diagram showing the structure of a communication channel of the cartridge for molecular detection in example 9;
FIG. 5 is a schematic diagram of the structure of a one-way PDMS soft valve of the cartridge for molecular assay in example 11;
FIG. 6 is a schematic diagram of the structure of a one-way PDMS soft valve of the cartridge for molecular assay in example 12.
Reference numerals illustrate:
1. a case body; 2. a nucleic acid extraction unit; 21. a sample inlet; 212. a sample inlet cover; 22. a cleavage zone; 221. a lysate vesicle; 222. magnetic bead vesicles; 23. a first cleaning zone; 231. a first cleaning fluid vesicle; 24. a second cleaning zone; 241. a second cleaning fluid vesicle; 242. reactive enzyme vesicles; 3. a nucleic acid amplification unit; 31. a communication passage; 311. a unidirectional PDMS soft valve; 312. a sample inlet channel; 313. a sample outlet channel; 32. and a mixed liquor reaction zone.
Detailed Description
The present utility model will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent. It should be understood that the particular embodiments described herein are illustrative only and are not intended to limit the utility model, i.e., the embodiments described are merely some, but not all, of the embodiments of the utility model. The components of the embodiments of the present utility model generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be made by a person skilled in the art without making any inventive effort, are intended to be within the scope of the present utility model.
It is noted that relational terms such as "first" and "second", and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, terms such as "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" or the like is not excluded from a process, method, or the like that includes the element.
In the description of the present utility model, the terms "mounted," "connected," "coupled," and "connected," as may be used broadly, and may be connected, for example, fixedly, detachably, or integrally, unless otherwise specifically defined and limited; can be mechanically or electrically connected; either directly, indirectly through intermediaries, or in communication with the interior of the two elements. The specific meaning of the above terms in the present utility model can be understood by those skilled in the art in specific cases.
In the description of the present utility model, it should be noted that, unless explicitly stated and limited otherwise, the term "provided" may be interpreted broadly, and for example, an object "provided" may be a part of a body, may be separately disposed from the body, and may be connected to the body, where the connection may be a detachable connection or an undetachable connection. The specific meaning of the above terms in the present utility model can be understood by those skilled in the art in specific cases.
The present utility model is described in further detail below with reference to examples.
The present utility model provides a specific example 1 of a cartridge for molecular detection:
the cartridge for molecular detection provided in this embodiment includes a nucleic acid extraction portion, as shown in fig. 1 and 2, the nucleic acid extraction portion 2 includes a sample inlet 21, a sample inlet cover 212 is provided on the sample inlet 21, and the sample inlet cover 212 is in interference fit with the cartridge body 1, so as to ensure tightness of the position of the sample inlet 21. The sample inlet 21 is communicated with the cracking zone 22, the cracking zone 22 is provided with a cracking liquid accommodating structure and a magnetic bead accommodating structure, the cracking liquid accommodating structure is a cracking liquid vesicle 221, the magnetic bead accommodating structure is a magnetic bead vesicle 222, the cracking liquid vesicle 221, the magnetic bead vesicle 222 and the cracking zone 22 are concentrically arranged, the cracking liquid vesicle 221 protrudes out of the top surface of the cartridge to be arranged, and the magnetic bead vesicle 221 protrudes out of the cartridge to be arranged.
As shown in fig. 1 and 2, the cracking zone 22 is communicated with a cleaning zone, the cleaning zone is provided with a cleaning solution accommodating structure and a reactive enzyme accommodating structure, the cleaning solution accommodating structure in this embodiment is a cleaning solution vesicle, the reactive enzyme accommodating structure is a reactive enzyme vesicle 242, the cleaning zone is provided with two zones along the flowing direction of the sample, namely a first cleaning zone 23 and a second cleaning zone 24, the reactive enzyme vesicle 242 is arranged on the second cleaning zone 24, the first cleaning zone 23 and the second cleaning zone 24 are respectively provided with a cleaning solution vesicle, the second cleaning solution vesicle 241 and the reactive enzyme vesicle 242 on the second cleaning zone are concentrically arranged on the second cleaning zone 24, and the second cleaning solution vesicle 241 and the reactive enzyme vesicle 242 are respectively protruded out of the top surface and the bottom surface of the cartridge. The cracking area is designed into an expanded structure, so that the storage volume of a sample, magnetic beads and a cracking liquid can be met, and the fluctuation of the cracking liquid level possibly caused by different cell crushing modes can be met. And a space is reserved for the liquid level and the sample inlet cover, so that the functions of evaporation and overflow prevention and the like are achieved, and pollution is prevented.
As shown in fig. 1 and 2, a connection channel 31 is arranged on the cleaning area, the connection channel 31 is communicated with the nucleic acid amplification part 3, the nucleic acid amplification part 3 comprises four mixed liquid reaction areas 32, the connection channel 31 is provided with four connection channels 31, sample outlets of the four connection channels 31 are respectively connected with one mixed liquid reaction area 32, each connection channel 31 is provided with a valve, the valve in the embodiment is a unidirectional PDMS soft valve 311, the structure of the unidirectional PDMS soft valve 311 is as shown in fig. 3, the unidirectional PDMS soft valve 311 comprises two valve plates bent into circular arc shapes, one side of each valve plate is fixed on the inner wall of the connection channel 31, the other side of each valve plate is tightly attached to the other valve plate bent into circular arc shape, and the inner side of the bent circular arc shape of the valve plate faces the nucleic acid amplification part 3 so as to play a role of unidirectional conduction and prevent the mixed liquid in the nucleic acid amplification part 3 from flowing back into the cleaning area. Different primers are added to the four mixed liquor reaction zones 32 to amplify different accounting fragments, and the mixed liquor reaction zones 32 in the embodiment have a conical structure.
In this embodiment, the overall size of the cartridge is 50-80mm, the width is 20-50mm, the size of the reagent vesicle is 1-4mm, the width of the sample inlet 21 is 0.5-4mm, after the sample inlet cover 212 is matched with the cartridge body 1, a sealed environment is formed in the cartridge body 1, the cleaning of the internal environment of the cartridge body 1 can be ensured before sample injection, and after sample injection, the drifting of sample droplets can be avoided. The card box body 1 is made of hard plastic.
When detection is needed, the cartridge is put in the corresponding position of the detection instrument, the lysis solution vesicle 221, the magnetic bead vesicle 222, the cleaning solution vesicle and the reactive enzyme vesicle 242 are extruded to release the substances inside the cartridge body 1, the lysis solution, the cleaning solution and the reactive enzyme are insoluble in each other, the inside of the cartridge is naturally layered, the sample is injected into the cartridge body of the cartridge, firstly, the sample enters the lysis zone 22, the acquired sample cells are ruptured by physical heating and ultrasonic action to release DNA, substances such as nucleic acid, protein and cell fragments after cell lysis are coupled with the nano magnetic beads, the substances after cell lysis and the nano magnetic beads enter the cleaning zone together under the action of a magnetic field, the cleaning agent removes the cell fragments and the protein, finally, the complex of the reactive enzyme and the nano magnetic beads and the nucleic acid enters the nucleic acid amplification part 3 through the one-way soft valve 311 on the communication channel 31, the magnetic beads and the nucleic acid are separated under the action of the magnetic field, the magnetic beads are moved out of the nucleic acid amplification part 3, and the amplified substances are subjected to fluorescence detection, and the detection work of the amplified substances is completed, and the detection work of the nucleic acid is completed.
Specific example 2 of the cartridge for molecular detection provided by the present utility model:
the difference from the embodiment 1 is mainly that in the present embodiment, a reaction zone is provided behind the washing zone, the reactive enzyme vesicles are disposed on the reaction zone, and single-phase PDMS soft valves are disposed between the lysis zone and the washing zone and between the reaction zone, so that it is ensured that each vesicle is not mutually dissolved after releasing the internal agent, and the single-phase PDMS soft valve is opened under the pushing of the nano magnetic beads during the detection, so as to gradually perform the lysis, washing and extraction of the sample.
Specific example 3 of the cartridge for molecular detection provided by the present utility model:
the difference from embodiment 1 is mainly that in this embodiment, a unidirectional PDMS soft valve is provided between the cleaning area and the lysing area to separate different areas, so that the problem of mutual dissolution of solutions can be avoided, each vesicle can not be mutually dissolved after releasing the internal agent, and the PDMS soft valve is opened under the pushing of the nano magnetic beads during detection, so as to gradually perform the tasks of lysing, cleaning and extracting the sample.
Specific example 4 of the cartridge for molecular detection provided by the present utility model:
the difference from embodiment 1 is mainly that, in this embodiment, no magnetic bead vesicle is separately provided, the nano magnetic beads are put in the lysis zone in advance, and a one-way valve is provided between the lysis zone and the washing zone to ensure that the nano magnetic beads cannot enter the washing zone.
Specific example 5 of the cartridge for molecular detection provided by the present utility model:
the difference from example 1 is mainly that in this example, both the lysate vesicles and the magnetic bead vesicles are disposed adjacent to the lysis zone, and both the lysate vesicles and the magnetic bead vesicles are disposed protruding from the top surface of the cartridge. In other embodiments, the lysate vesicles and the magnetic bead vesicles may both be provided protruding from the bottom surface of the cartridge.
Specific example 6 of the cartridge for molecular detection provided by the present utility model:
the difference from embodiment 1 is mainly that in this embodiment, only one washing zone is provided, on which washing solution vesicles and reactive enzyme vesicles are provided, the washing solution vesicles and the reactive enzyme vesicles are arranged concentrically, and the washing solution vesicles and the reactive enzyme vesicles are provided protruding from the top surface and the bottom surface of the cartridge, respectively.
Specific example 7 of the cartridge for molecular detection provided by the present utility model:
the main difference from example 1 is that in this example, both the washing liquid vesicles and the reactive enzyme vesicles on the second washing zone are disposed adjacently on the second washing zone; the cleaning solution vesicles and the reactive enzyme vesicles on the second cleaning zone are both arranged protruding from the bottom surface of the cartridge, and in other embodiments, the cleaning solution vesicles and the reactive enzyme vesicles on the second cleaning zone may be arranged protruding from the top surface of the cartridge.
Specific example 8 of the cartridge for molecular detection provided by the present utility model:
the difference from example 1 is mainly that in this example, two mixed solution reaction regions are provided in the nucleic acid amplification section, and the types of primers in the two mixed solution reaction regions are different, so that two different nucleic acid fragments can be amplified simultaneously, and whether or not the two nucleic acid fragments exist in the sample can be detected. In other embodiments, the number of mixed liquor reaction zones may be three, four, five or other numbers, and the number of specific mixed liquor reaction zones may be set according to the number of nucleic acid fragments detected by design.
Specific example 9 of the cartridge for molecular detection provided by the present utility model:
the difference from embodiment 1 is mainly that in this embodiment, the connection channel is composed of two parts, including a single channel sample inlet channel connected to the washing area and four sample outlet channels connected to the reaction areas of the mixed solution, the valve for controlling the on-off of the connection channel is only one and on the sample inlet channel, the valve in this embodiment is a wax valve, the wax valve is opened under the heating of the heating module in the detecting instrument, the magnetic nano magnetic beads enter the amplifying part together with the extracted nucleic acid under the action of the magnetic field, and the structure of the connection channel in this embodiment is as shown in fig. 4. In other embodiments the valve may also be an electrically operated valve or a wax valve.
Specific example 10 of the cartridge for molecular detection provided by the present utility model:
the difference from embodiment 1 is mainly that in this embodiment, the communication channel is composed of two parts, including a single channel sample inlet channel connected to the cleaning zone and four sample outlet channels connected to the reaction zones of the mixed solution, and a valve for controlling the on-off of the communication channel is disposed on each sample outlet channel, and in this embodiment, the valve is an electric valve, and in other embodiments, the valve may be a unidirectional PDMS soft valve or a wax valve.
Specific example 11 of the cartridge for molecular detection provided by the present utility model:
the difference from embodiment 1 is mainly that, in this embodiment, the structure of the single-phase PDMS soft valve 311 is shown in fig. 5, and the single-phase PDMS soft valve includes a piece of valve plate bent into a circular arc shape and a piece of planar valve plate, where one ends of the planar valve plate and the valve plate bent into a circular arc shape are fixed on the inner wall of the communication channel, the inner side of the circular arc-shaped valve plate faces the cleaning area, and the other side of the circular arc-shaped valve plate abuts against one side of the planar valve plate facing away from the cleaning area.
Specific example 12 of the cartridge for molecular detection provided by the present utility model:
the difference from embodiment 1 is mainly that, in this embodiment, the structure of the single-phase PDMS soft valve 311 is shown in fig. 6, and the single-phase PDMS soft valve includes a piece of valve plate bent into a circular arc shape, where one side of the circular arc-shaped valve plate is fixed on the inner wall of the communication channel, and the other side of the circular arc-shaped valve plate is abutted against the inner wall of the communication channel.
Specific example 13 of the cartridge for molecular detection provided by the present utility model:
the main difference from embodiment 1 is that in this embodiment, the material of the cartridge body is PP hard plastic.
Specific example 14 of a cartridge for molecular detection provided by the present utility model:
the main difference from embodiment 1 is that in this embodiment, the material of the cartridge body is PC hard plastic. In other embodiments, the material of the cartridge may be other kinds of plastics.
Specific example 15 of the cartridge for molecular detection provided by the present utility model:
the difference from embodiment 1 is mainly that in this embodiment, the mixed liquor reaction zone has a square structure, and in other embodiments, the mixed liquor reaction zone may have other shapes.
It should be noted that the above-mentioned embodiments are merely preferred embodiments of the present utility model, and the present utility model is not limited to the above-mentioned embodiments, but may be modified without inventive effort or equivalent substitution of some of the technical features thereof by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (10)
1. The cartridge for molecular detection is characterized in that a reagent vesicle is arranged on the cartridge, an extrusion rupture membrane is arranged on one side of the reagent vesicle facing the inside of the cartridge body (1), and the extrusion rupture membrane ruptures under the action of external force to release the reagent in the reagent vesicle during detection.
2. The cartridge for molecular detection according to claim 1, wherein the cartridge comprises a nucleic acid extraction portion (2), a communication channel (31) and a washing region connected to a nucleic acid amplification portion (3) are provided on the nucleic acid extraction portion (2), and a valve is provided on the communication channel (31); the reagent vesicles are provided on the nucleic acid extraction unit (2).
3. The cartridge for molecular detection according to claim 2, wherein a cleavage region (22) is provided on the nucleic acid extraction portion (2), the cleavage region (22) communicates with the washing region, and the communication channel (31) is provided on the washing region; the reagent vesicles on the lysis zone (22) are lysis solution vesicles (221) for containing lysis solution, and the reagent vesicles on the cleaning zone are cleaning solution vesicles for containing cleaning solution.
4. A cartridge for molecular detection according to claim 3, wherein the washing zone is further provided with a reagent vesicle for accommodating a reaction enzyme, the reagent vesicle for accommodating a reaction enzyme is a reaction enzyme vesicle (242), the reaction enzyme is insoluble in the washing liquid, and the lysate is insoluble in both the reaction enzyme and the washing liquid.
5. The cartridge for molecular detection according to claim 4, wherein the cleavage region (22) is further provided with a reagent vesicle for accommodating a nanomagnetic bead, and the reagent vesicle for accommodating a nanomagnetic bead is a magnetic bead vesicle (222).
6. The cartridge for molecular detection according to claim 5, wherein the reagent vesicles are spherical vesicles.
7. The cartridge for molecular detection according to claim 6, wherein the lysate vesicle (221), the magnetic bead vesicle (222) and the cleavage zone (22) are arranged concentrically, and the lysate vesicle (221) and the magnetic bead vesicle (222) are provided protruding from the top surface and the bottom surface of the cartridge, respectively; the cleaning solution vesicles and the reactive enzyme vesicles (242) are concentrically arranged, and the cleaning solution vesicles and the reactive enzyme vesicles (242) are respectively protruded out of the top surface and the bottom surface of the cartridge.
8. The cartridge for molecular detection according to any one of claims 4 to 7, wherein two washing zones are provided along the flow direction of the sample, namely a first washing zone (23) and a second washing zone (24), wherein a first washing liquid vesicle (231) and a second washing liquid vesicle (241) are provided on the first washing zone (23) and the second washing zone (24), respectively, and wherein the reactive enzyme vesicle (242) is provided only on the second washing zone (24).
9. The cartridge for molecular detection according to claim 2, wherein the nucleic acid amplification section (3) comprises at least two mixed solution reaction regions (32), and the types of primers in any two mixed solution reaction regions (32) are different; the communication channel (31) between the washing area and the nucleic acid amplification part (3) is provided with a plurality of sample outlets which are respectively communicated with the corresponding mixed solution reaction areas (32).
10. The cartridge for molecular detection according to claim 9, wherein the valve on the communication channel (31) is a wax valve or a one-way PDMS soft valve (311), and when the valve on the communication channel (31) is the wax valve, the cartridge is heated to open the wax valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320114218.4U CN219709478U (en) | 2023-01-17 | 2023-01-17 | Cartridge for molecular detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320114218.4U CN219709478U (en) | 2023-01-17 | 2023-01-17 | Cartridge for molecular detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219709478U true CN219709478U (en) | 2023-09-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320114218.4U Active CN219709478U (en) | 2023-01-17 | 2023-01-17 | Cartridge for molecular detection |
Country Status (1)
| Country | Link |
|---|---|
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117783250A (en) * | 2024-02-26 | 2024-03-29 | 中国人民解放军总医院 | Electrochemical sensing micro-fluidic cartridge based on quantitative detection of alpha defensin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117783250A (en) * | 2024-02-26 | 2024-03-29 | 中国人民解放军总医院 | Electrochemical sensing micro-fluidic cartridge based on quantitative detection of alpha defensin |
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