CN219670512U - Heating device for nucleic acid reagent extraction instrument - Google Patents
Heating device for nucleic acid reagent extraction instrument Download PDFInfo
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- CN219670512U CN219670512U CN202321207012.2U CN202321207012U CN219670512U CN 219670512 U CN219670512 U CN 219670512U CN 202321207012 U CN202321207012 U CN 202321207012U CN 219670512 U CN219670512 U CN 219670512U
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- nucleic acid
- extraction
- acid reagent
- heating
- signal
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- 238000010438 heat treatment Methods 0.000 title claims abstract description 88
- 238000000605 extraction Methods 0.000 title claims abstract description 69
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 43
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 43
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 43
- 239000012445 acidic reagent Substances 0.000 title claims abstract description 41
- 238000001514 detection method Methods 0.000 claims abstract description 20
- 239000002390 adhesive tape Substances 0.000 claims description 9
- 230000000903 blocking effect Effects 0.000 claims description 7
- 238000005485 electric heating Methods 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 5
- 230000002093 peripheral effect Effects 0.000 claims description 4
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 8
- 238000011330 nucleic acid test Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Natural products C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002918 waste heat Substances 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a heating device for a nucleic acid reagent extraction instrument, which comprises an extraction instrument body, wherein a control screen is arranged on the extraction instrument body, an extraction cavity for placing a nucleic acid reagent to be extracted is formed in the extraction instrument body, a placement opening communicated with the extraction cavity and the outside is formed in the extraction instrument body, a cabin door for opening and closing the placement opening is also arranged on the extraction instrument body, a plurality of heating pieces are arranged on the inner wall of the extraction cavity, and a temperature control circuit for controlling the opening and the closing of the heating pieces is coupled with the heating pieces, and the temperature control circuit comprises: the temperature control circuit comprises a temperature sensor, a comparison unit and a switch unit. The utility model has the effect of improving the detection accuracy of the nucleic acid reagent extractor.
Description
Technical Field
The utility model relates to the field of biological sample extraction, in particular to a heating device for a nucleic acid reagent extraction instrument.
Background
The nucleic acid reagent extractor is an analytical instrument in the field of biology, is widely applied to biological extraction and biochemical analysis operations, is a high-tech instrument composed of a plurality of structures, and comprises heating devices in a plurality of component structures, and because nucleic acid extracting solution is easy to crystallize at low temperature, the heating devices are required to provide necessary working temperature for the nucleic acid extracting solution.
The existing nucleic acid reagent extractor generally fixes the extraction time in the biological extraction process to ensure that enough samples are extracted, and in the general extraction process, the heating time is the same as the extraction time, if the extraction time is longer, the sample in the nucleic acid reagent extractor is damaged due to the fact that the heating temperature is too high, and the detection accuracy of the nucleic acid reagent extractor is reduced, so that improvement is needed.
Disclosure of Invention
The utility model provides a heating device for a nucleic acid reagent extractor, which aims to improve the detection accuracy of the nucleic acid reagent extractor.
The utility model provides a heating device for a nucleic acid reagent extractor, which adopts the following technical scheme:
the utility model provides a heating device for nucleic acid reagent draws appearance, includes draws the appearance body, draw the appearance body to install and control the screen, draw the appearance body to offer and be used for placing the extraction chamber of waiting to draw nucleic acid reagent, draw the appearance body to offer the intercommunication draw chamber and external mouth of placing, draw the appearance body still to install and be used for opening and closing place the hatch door of mouth, draw the inner wall in chamber and install a plurality of heating pieces, the heating piece coupling has be used for controlling the control by temperature change circuit that the heating piece opened and close, the control by temperature change circuit includes: the temperature control circuit comprises a temperature sensor, a comparison unit and a switch unit;
the temperature sensor is fixed on the inner wall of the extraction cavity, and is used for detecting the temperature in the extraction cavity and sending out a detection signal;
the comparison unit is coupled to the temperature sensor and is provided with a threshold signal VREF, so as to send out a comparison signal when the detection signal is greater than the threshold signal VREF; when the detection signal is smaller than the threshold signal VREF, a comparison signal is not sent out;
the switch unit is coupled to the comparison unit and connected in series in the power supply loop of the heating element, so as to send a disconnection signal to disconnect the power supply loop of the heating element when receiving the comparison signal.
Through adopting above-mentioned technical scheme, close through the hatch door and place the mouth, start nucleic acid reagent extractor, the work of nucleic acid reagent extractor this moment, extract sample and heating piece start-up work, temperature sensor real-time monitoring draws the intracavity temperature this moment, when drawing intracavity temperature to be higher than the setting value, comparison unit sends comparison signal to switch unit, switch unit control heating piece's power supply loop disconnection, control heating piece stop work promptly, thereby make heating piece stop heating, when making extraction time longer, the difficult continuous work of heating piece is to the messenger draws the intracavity temperature too high and damages the sample, improve nucleic acid reagent extractor's detection accuracy.
Preferably, the comparing unit includes a comparator N1, a first signal input end of the comparator N1 is coupled to the temperature sensor, a second signal input end of the comparator N1 is connected to the threshold signal VREF, and a signal output end of the comparator N1 is coupled to the switching unit.
Through adopting above-mentioned technical scheme, comparator N1 is with the threshold value signal VREF of first signal input access detection signal with second signal input in real time and is compared and output the comparison signal, in time carries out the comparison of signal and then the switching unit output switch signal, realizes the comparison function to the size through comparator N1.
Preferably, the switching unit includes a transistor Q1, a base of the transistor Q1 is coupled to a signal output end of the comparator N1, an emitter of the transistor Q1 is coupled to the power voltage VCC, and a collector of the transistor Q1 is grounded.
By adopting the technical scheme, when the base electrode of the triode Q1 receives the comparison signal, the base electrode of the triode Q1 is converted from low level to high level, the triode Q1 is conducted and sends a closing signal to control the heating element to be closed; when the base electrode of the triode Q1 does not receive the comparison signal, the base electrode of the triode Q1 maintains a low level, the triode Q1 is not conducted, the heating piece is kept working, and the function of sending a switching signal by the switching unit is achieved.
Preferably, the switch unit further comprises a relay KM1, a coil of the relay KM1 is connected with a collector of the triode Q1 in series and then grounded, the relay KM1 comprises a normally-closed contact switch KM1-1, and the normally-closed contact switch KM1-1 is connected in series in a power supply loop of the heating element.
By adopting the technical scheme, when the triode Q1 is conducted, the coil of the relay KM1 is electrified, the normally-closed contact switch KM1-1 is disconnected, and the power supply loop of the heating element is disconnected, namely the heating element stops working; when the triode Q1 is disconnected, the coil of the relay KM1 is powered off, the normally-closed contact switch KM1-1 is closed again, and the power supply loop of the heating element is conducted, namely the heating element works, so that the function of controlling the heating element to automatically obtain power failure is realized
Preferably, the heating elements are electric heating tubes, the electric heating tubes are respectively arranged on two side walls and the bottom wall of the extraction cavity, and the electric heating tubes are arranged on the inner wall of the extraction cavity in a snake-shaped winding manner.
Through adopting above-mentioned technical scheme, the electrothermal tube sets up respectively at both sides wall and the diapire of extracting the chamber, and is the electrothermal tube that the serpentine set up and make the position that generates heat even, and the intracavity heating position is even promptly extracted, is difficult for making the temperature of local position too high because of concentrated heating, makes the sample impaired or makes the misjudgement of temperature control circuit lead to the heating abundant.
Preferably, a blocking net is further fixed on the inner wall of the extraction cavity, and the blocking net is covered on the heating element.
By adopting the technical scheme, when the extraction work is just finished, the temperature of the electric heating tube is higher, the net is blocked, the workers are protected from being scalded, and the safety is improved.
Preferably, the extractor body is provided with the subassembly of placing that is used for placing nucleic acid reagent, places the subassembly and includes two guide rails and is used for placing nucleic acid reagent place the board, two the guide rail sets up respectively the relative both sides wall in extraction cavity, place the relative both sides of board respectively with two the guide rail one-to-one joint.
Through adopting above-mentioned technical scheme, place the board and install in the guide rail through the joint, be convenient for change the board of placing of different specifications, make the extractor satisfy the nucleic acid test paper that awaits measuring of different demand specifications.
Preferably, an adhesive tape is installed on one side of the cabin door, which is close to the extraction cavity, and when the cabin door is closed, the adhesive tape is abutted against the peripheral wall of the placement opening.
Through adopting above-mentioned technical scheme, when the hatch door was closed, the adhesive tape was supported tightly with the perisporium of placing the mouth, made to place and be difficult for leaving the clearance between mouth perisporium and the hatch door, avoided extracting the in-process and draw the chamber to keep airtight, improved the detection accuracy.
In summary, the present utility model includes at least one of the following beneficial technical effects:
1. when the temperature in the extraction cavity is higher than a set value, the comparison unit sends a comparison signal to the switch unit, and the switch unit controls the power supply loop of the heating element to be disconnected, namely controls the heating element to stop working, so that the heating element stops heating, and when the extraction time is longer, the heating element is not easy to continuously work until the temperature in the extraction cavity is too high to damage a sample, and the detection accuracy of the nucleic acid reagent extractor is improved;
2. the heating part in the extraction cavity is uniform, so that the temperature of the local position is not easy to be too high due to concentrated heating, and the sample is damaged or the temperature control circuit misjudges to cause sufficient heating;
3. when the cabin door is closed, the adhesive tape is abutted against the peripheral wall of the placing port, so that a gap is not easy to leave between the peripheral wall of the placing port and the cabin door, the extraction cavity is prevented from being kept airtight in the extraction process, and the detection accuracy is improved.
Drawings
Fig. 1 is a schematic view showing the overall structure of an opened state of a door according to an embodiment of the present utility model.
Figure 2 is an overall front view of the door of the embodiment of the present utility model in an open position.
Fig. 3 is a schematic view of a portion of the structure of the placement plate under the placement plate according to the embodiment of the present utility model.
Fig. 4 is a circuit diagram of a temperature control circuit according to an embodiment of the utility model.
Reference numerals illustrate:
1. an extractor body; 2. a control panel; 3. an extraction chamber; 4. a placement port; 5. a cabin door; 6. a handle; 7. an adhesive tape; 8. a heating member; 9. a blocking net; 10. a hoop; 11. placing the assembly; 111. a guide rail; 112. placing a plate; 12. an extraction assembly; 13. a temperature sensor; 14. a comparison unit; 15. a switching unit; 16. and a limiting block.
Detailed Description
The utility model is described in further detail below with reference to fig. 1-4.
The embodiment of the utility model discloses a heating device for a nucleic acid reagent extractor. Referring to fig. 1 and 2, a heating device for nucleic acid reagent draws appearance, including extracting appearance body 1, control screen 2 is installed at the top of extracting appearance body 1, extracting appearance body 1 has been seted up and has been used for placing the extraction chamber 3 of waiting to extract nucleic acid reagent, extracting appearance body 1 has been seted up and has been linked together and has been extracted chamber 3 and external the mouth 4 of placing, it still installs the hatch door 5 that is used for opening and closing and place mouth 4 to extract appearance body 1, hatch door 5 is fixed with handle 6, hatch door 5 is close to one side of extracting chamber 3 and installs adhesive tape 7, when hatch door 5 is closed, adhesive tape 7 supports with the perisporium of placing mouth 4 tightly, make and place and be difficult for leaving the clearance between mouth 4 perisporium and the hatch door 5, it keeps airtight to avoid extracting the chamber 3 in the extraction process, improve the detection accuracy.
Referring to fig. 2 and 3, three heating elements 8 are installed on the inner wall of the extraction cavity 3, the three heating elements 8 are all electrothermal tubes which are arranged in a serpentine shape, the three electrothermal tubes are respectively fixed on two side walls and the bottom wall of the extraction cavity 3 through anchor clamps 10, three blocking nets 9 are also fixed on the inner wall of the extraction cavity 3, and the three blocking nets 9 are respectively covered on the three electrothermal tubes to protect workers from being scalded by waste heat of the electrothermal tubes.
Referring to fig. 1 and 2, the extractor body 1 is further provided with a placement component 11 for placing a nucleic acid reagent and an extraction component 12 for extracting a sample, the placement component 11 includes two guide rails 111 and a placement plate 112 for placing the nucleic acid reagent, the two guide rails 111 are respectively disposed on two opposite side walls of the extraction cavity 3, in this embodiment, the guide rails 111 are concavely disposed on an inner side wall of the extraction cavity 3, two opposite sides of the placement plate 112 are respectively clamped with the two guide rails 111 in a one-to-one correspondence manner, the placement plate 112 is assembled and disassembled along the depth direction of the extraction cavity 3 under the combined action of the two guide rails 111, the replacement of the placement plate 112 is facilitated, and a limiting block 16 forming a placement groove is further fixed on the top surface of the placement plate 112.
Referring to fig. 2 and 4, the heating element 8 is coupled with a temperature control circuit for controlling the heating element 8 to be turned on or off, and the temperature control circuit includes a temperature sensor 13, a comparing unit 14, and a switching unit 15. A temperature sensor 13 is fixed to the side wall of the extraction chamber 3, and the temperature sensor 13 is used for detecting the temperature in the extraction chamber 3 and sending out a detection signal.
Referring to fig. 4, the comparing unit 14 is coupled to the temperature sensor 13 and is provided with a threshold signal VREF to emit a comparison signal when the detection signal is greater than the threshold signal; the comparing unit 14 includes a comparator N1, a first signal input terminal of the comparator N1 is a normal phase input terminal, the normal phase input terminal is coupled to the temperature sensor 13 at the same time, a second signal input terminal of the comparator N1 is an inverted input terminal, the inverted input terminal is connected to the threshold signal VREF, and a signal output terminal of the comparator N1 is coupled to the switching unit 15.
Referring to fig. 4, the switch unit 15 is coupled to the comparing unit 14 and is connected in series in the power supply circuit of the heating element 8, so that when the switch unit 15 receives the comparing signal, the power supply circuit of the heating element 8 is turned off, and when the switch unit receives the closing signal, the power supply circuit of the heating element 8 is kept on; the switch unit 15 includes a PNP-type triode Q1 and a relay KM1, where a base electrode of the triode Q1 is coupled to a signal output end of the comparator N1, an emitter electrode of the triode Q1 is coupled to a power supply voltage VCC, a collector electrode of the triode Q1 is connected in series with a coil of the relay KM1 and then grounded, the relay KM1 includes a normally-closed contact switch KM1-1, the normally-closed contact switch KM1-1 is connected in series in a power supply loop of the heating element 8, and a switch S1 is connected in series in the power supply loop of the heating element 8, when the extractor works, the switch S1 is closed, and when the extractor does not work, the switch S1 remains open.
The embodiment of the utility model provides a heating device for a nucleic acid reagent extractor, which comprises the following implementation principles: after the placing plate 112 with the nucleic acid test paper to be tested is clamped on the guide rail 111, the cabin door 5 is closed, the placing opening 4 is closed through the cabin door 5, and the nucleic acid reagent extractor is started, so that the nucleic acid reagent extractor works.
When the extraction cavity 3 works, the switch S1 is closed, the power supply loop of the heating element 8 is conducted, the temperature sensor 13 monitors the temperature in the extraction cavity 3 in real time, when the temperature in the extraction cavity 3 is higher than the value set by the temperature sensor 13, the detection signal is larger than the threshold signal VREF, the comparison unit 14 sends a closing signal to the switch unit 15, and the power supply loop of the heating element 8 is disconnected, so that the heating element 8 stops working; namely, after the heating element 8 is heated to the set temperature, the heating element 8 automatically stops working, so that the heating element 8 stops heating, and when the extraction time is longer, the heating element 8 is not easy to continuously work until the temperature in the extraction cavity 3 is overhigh to damage a sample, and the detection accuracy of the nucleic acid reagent extractor is improved.
When the detected value is lower than the set value of the temperature sensor 13, the detection signal is smaller than the threshold signal VREF, the comparison unit 14 does not send out a comparison signal, the power supply loop of the heating element 8 is unchanged, at the moment, if the switch S1 is closed, the power supply loop of the heating element 8 is conducted and the heating element 8 is operated, and if the switch S1 is opened, the power supply loop of the heating element 8 is disconnected and the heating element 8 is not operated.
The above embodiments are not intended to limit the scope of the present utility model, so: all equivalent changes in structure, shape and principle of the utility model should be covered in the scope of protection of the utility model.
Claims (8)
1. The utility model provides a heating device for nucleic acid reagent draws appearance, its characterized in that, including extracting appearance body (1), it controls screen (2) to extract appearance body (1) installs, it offers extraction chamber (3) that are used for placing the nucleic acid reagent that waits to extract appearance body (1), it offers the intercommunication to extract appearance body (1) place mouth (4) outside extraction chamber (3), it still installs and be used for opening and closing hold hatch door (5) of mouth (4) to extract appearance body (1), a plurality of heating piece (8) are installed to the inner wall of extraction chamber (3), heating piece (8) coupling has be used for controlling the control by temperature change circuit that heating piece (8) opened and close, the control by temperature change circuit includes: a temperature sensor (13), a comparison unit (14) and a switching unit (15);
the temperature sensor (13) is fixed on the inner wall of the extraction cavity (3), and the temperature sensor (13) is used for detecting the temperature in the extraction cavity (3) and sending out a detection signal;
the comparison unit (14) is coupled to the temperature sensor (13) and is provided with a threshold signal VREF to send out a comparison signal when the detection signal is greater than the threshold signal VREF; when the detection signal is smaller than the threshold signal VREF, a comparison signal is not sent out;
the switch unit (15) is coupled to the comparison unit (14) and is connected in series in the power supply circuit of the heating element (8) so as to send a disconnection signal to disconnect the power supply circuit of the heating element (8) when receiving the comparison signal.
2. The heating device for a nucleic acid reagent extraction instrument according to claim 1, wherein the comparing unit (14) comprises a comparator N1, a first signal input terminal of the comparator N1 is coupled to the temperature sensor (13), a second signal input terminal of the comparator N1 is connected to the threshold signal VREF, and a signal output terminal of the comparator N1 is coupled to the switching unit (15).
3. The heating device for a nucleic acid reagent extraction apparatus according to claim 2, wherein the switching unit (15) comprises a transistor Q1, a base of the transistor Q1 is coupled to a signal output terminal of the comparator N1, an emitter of the transistor Q1 is coupled to a power supply voltage VCC, and a collector of the transistor Q1 is grounded.
4. A heating device for a nucleic acid reagent extractor according to claim 3, wherein the switch unit (15) further comprises a relay KM1, the coil of the relay KM1 is connected in series with the collector of the triode Q1 and then grounded, the relay KM1 comprises a normally closed contact switch KM1-1, and the normally closed contact switch KM1-1 is connected in series in the power supply circuit of the heating element (8).
5. The heating device for the nucleic acid reagent extraction instrument according to claim 1, wherein the heating elements (8) are electric heating tubes, the electric heating tubes are respectively arranged on two side walls and the bottom wall of the extraction cavity (3), and the electric heating tubes are arranged on the inner wall of the extraction cavity (3) in a serpentine shape.
6. The heating device for a nucleic acid reagent extraction instrument according to claim 1, wherein a blocking net (9) is further fixed on the inner wall of the extraction chamber (3), and the blocking net (9) is covered on the heating element (8).
7. The heating device for a nucleic acid reagent extraction instrument according to claim 1, wherein the instrument body (1) is provided with a placement component (11) for placing a nucleic acid reagent, the placement component (11) comprises two guide rails (111) and a placement plate (112) for placing the nucleic acid reagent, the two guide rails (111) are respectively arranged on two opposite side walls of the extraction cavity (3), and two opposite sides of the placement plate (112) are respectively clamped with the two guide rails (111) in a one-to-one correspondence manner.
8. The heating device for a nucleic acid reagent extraction instrument according to claim 1, wherein an adhesive tape (7) is mounted on a side of the door (5) close to the extraction chamber (3), and when the door (5) is closed, the adhesive tape (7) abuts against a peripheral wall of the placement port (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321207012.2U CN219670512U (en) | 2023-05-18 | 2023-05-18 | Heating device for nucleic acid reagent extraction instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321207012.2U CN219670512U (en) | 2023-05-18 | 2023-05-18 | Heating device for nucleic acid reagent extraction instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219670512U true CN219670512U (en) | 2023-09-12 |
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ID=87928907
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321207012.2U Active CN219670512U (en) | 2023-05-18 | 2023-05-18 | Heating device for nucleic acid reagent extraction instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219670512U (en) |
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2023
- 2023-05-18 CN CN202321207012.2U patent/CN219670512U/en active Active
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