CN219670484U - High-temperature heating assembly and double heating assemblies of real-time fluorescence PCR analyzer - Google Patents

Abstract

The utility model provides a high-temperature heating component and a double-heating component of a real-time fluorescence PCR analyzer, wherein the double-heating component comprises a high-temperature heating component and a low-temperature heating component which are arranged on a bottom plate of the tester, the high-temperature heating component is used for heating a microfluidic chip at high temperature, and the low-temperature heating component is used for heating the microfluidic chip at low temperature; the low-temperature heating assembly comprises a left low-temperature heating assembly, a right low-temperature heating assembly and a second driving mechanism, wherein the left low-temperature heating assembly is fixedly arranged on the bottom plate of the tester, the right low-temperature heating assembly is slidably arranged on the bottom plate of the tester, and a low-temperature heating area is formed between the left low-temperature heating assembly and the right low-temperature heating assembly. The utility model can realize rapid temperature rise and temperature reduction of the micro-fluidic chip and effectively prolong the service life of the tester; meanwhile, the temperature rise and the temperature reduction are not required to be switched at the same station, so that the detection time is shortened.

Description

High-temperature heating assembly and double heating assemblies of real-time fluorescence PCR analyzer

Technical Field

The utility model relates to the technical field of real-time fluorescence PCR analysis equipment, in particular to a high-temperature heating component, and further relates to a double-heating component of a real-time fluorescence PCR analyzer.

Background

The application number is: CN202222107463.0, publication number: the utility model of CN218742049U discloses an integrated qPCR micro-fluidic chip structure (hereinafter referred to as prior art 1), which comprises a qPCR micro-fluidic chip body, a hard film and a soft film, wherein the qPCR micro-fluidic chip body is provided with a sample adding port, a working cavity, a waste liquid cavity, a lysate cavity, an eluent cavity and a plurality of cleaning liquid cavities; the sample adding port is provided with a detachable sample rubber plug, and the sample adding port, the cracking liquid cavity, the cleaning liquid cavity, the eluent cavity and the waste liquid cavity are respectively communicated with the working cavity through a sample runner, a cracking liquid runner, a cleaning liquid runner, an eluent runner and a waste liquid runner; the inside of the lysis solution cavity, the cleaning solution cavity and the eluent cavity is provided with a solution package and a puncturing structure for puncturing the solution package.

When the microfluidic chip disclosed in the prior art 1 is used for real-time fluorescence, the microfluidic chip needs to be heated, and the microfluidic chip needs to be heated and cooled for a plurality of times, namely, high-temperature heating and low-temperature heating (cooling) cycle switching is realized, so that the sample meets the requirement of fluorescence detection; when the tester in the prior art heats and cools the microfluidic chip, one station is adopted for operation, high-temperature heating is realized at the same station, low-temperature heating is also realized, the heating device needs to realize switching between heating and cooling, the time required by heating and cooling is long, and the service life of the heating device is reduced; and the switching between heating and cooling is realized, the time spent is long, and the time spent is long in the process of high-low temperature cyclic heating of the microfluidic chip.

Disclosure of Invention

The utility model aims to provide a high-temperature heating assembly and a dual-heating assembly of a real-time fluorescence PCR analyzer, which adopt two devices for high-temperature heating and low-temperature heating in actual use, can realize rapid heating and cooling of a microfluidic chip, can effectively prolong the service life of the tester, and simultaneously, does not need to realize switching between heating and cooling at the same station, thereby shortening the detection time.

In order to solve the technical problems, the utility model adopts the following technical scheme:

the high-temperature heating assembly comprises a left high-temperature heating assembly, a right high-temperature heating assembly and a first driving mechanism, wherein the left high-temperature heating assembly is fixedly arranged on a bottom plate of the tester, a high-temperature heating area is formed between the left high-temperature heating assembly and the right high-temperature heating assembly, the right high-temperature heating assembly is arranged on the bottom plate of the tester in a sliding manner, and the first driving mechanism is used for driving the right high-temperature heating assembly to be close to or far away from the microfluidic chip;

the right side high temperature heating assembly is provided with a sample heating assembly in a sliding mode, the sample heating assembly is arranged at the upper end of the right side high temperature heating assembly in a sliding mode, and a reset spring is arranged between the sample heating assembly and the right side high temperature heating assembly.

Wherein the left and right high-temperature heating components comprise a heating plate mounting seat and a heating unit fixedly arranged on the heating plate mounting seat,

the heating plate mounting seat in the left high-temperature heating assembly is fixedly arranged on the bottom plate of the tester;

the right side high temperature heating component further comprises a second supporting seat and a second spring, the second supporting seat is fixedly arranged on the bottom plate of the tester, the heating plate installation seat in the right side high temperature heating component is slidably installed on the second supporting seat, two ends of the second spring are respectively connected with the second supporting seat and the heating plate installation seat in the right side high temperature heating component, and under the action of the second spring, the heating plate installation seat in the right side high temperature heating component is contacted with the first driving mechanism.

Further preferably, a second sliding groove is formed in the second supporting seat, the heating plate mounting seat in the right high-temperature heating assembly is arranged in the second sliding groove in a sliding mode through the sliding part, and the second spring is located in the second sliding groove.

Wherein, sample heating assembly includes sample heating mount pad and hot plate, and the hot plate is installed on sample heating mount pad, and sample heating mount pad realizes sliding connection through the sliding tray that sets up on the hot plate mount pad in the high temperature heating assembly in the right side and protruding in sample heating mount pad bottom, reset spring is located the sliding tray.

Wherein, the heating unit includes ceramic heating piece and laminating the heat transfer plate on ceramic heating piece, and ceramic heating piece is fixed to be set up on the hot plate mount pad.

Further defined, the guide hole is arranged on the heating plate mounting seat in the left side high-temperature heating component, the guide rod is arranged on the heating plate mounting seat in the right side high-temperature heating component, and the guide rod is matched with the guide hole.

Further preferably, the first driving mechanism comprises a first cam, a mounting cylinder fixedly mounted on the bottom plate of the tester and a first gear motor fixedly arranged on the mounting cylinder, an output shaft of the first gear motor extends into the mounting cylinder and then is connected with the first cam, and the first cam is in contact with a heating plate mounting seat in the right high-temperature heating assembly.

Wherein, the rotation is provided with the rotary drum on the hot plate mount pad in the high temperature heating subassembly on right side, and rotary drum side and first cam side contact.

The utility model also discloses a double heating component of the real-time fluorescence PCR analyzer, which comprises a high-temperature heating component and a low-temperature heating component which are arranged on a bottom plate of the tester, wherein the high-temperature heating component is used for heating the microfluidic chip at high temperature, and the low-temperature heating component is used for heating the microfluidic chip at low temperature;

the low-temperature heating assembly comprises a left low-temperature heating assembly, a right low-temperature heating assembly and a second driving mechanism, wherein the left low-temperature heating assembly is fixedly arranged on the bottom plate of the tester, the right low-temperature heating assembly is slidably arranged on the bottom plate of the tester, and a low-temperature heating area is formed between the left low-temperature heating assembly and the right low-temperature heating assembly; the second driving mechanism is used for driving the right-side low-temperature heating component to be close to or far away from the left-side low-temperature heating component.

Compared with the prior art, the utility model has the following beneficial effects:

the utility model mainly discloses a high-temperature heating assembly, which is used for heating a microfluidic chip, in actual use, after the microfluidic chip moves to a high-temperature heating area, the right-side high-temperature heating assembly is close to the microfluidic chip under the action of a first driving mechanism and is in contact with the microfluidic chip, and at the moment, the microfluidic chip can be directly heated; meanwhile, the sample in the sample cavity in the microfluidic chip can be preheated through the arranged sample heating assembly; the heating efficiency of the micro-fluidic chip can be effectively improved;

in addition, the utility model also discloses a double heating component of the real-time fluorescence PCR analyzer, which is used for realizing the rapid switching of high-temperature heating and low-temperature heating of the microfluidic chip, avoiding the switching of high temperature and low temperature at one station, effectively prolonging the service life of the heating component, simultaneously, the high-temperature heating and the low-temperature heating are separately carried out, so that the high-temperature heating component is always at a higher temperature, the low-temperature heating component is always at a temperature at the bottom of the intersection, the time of switching the high temperature and the low temperature of the same heating device is shortened, the heating time is further effectively shortened, and the detection efficiency is improved.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of the overall structure of the present utility model.

FIG. 2 is a schematic view showing the state of the utility model mounted on a tester base plate.

FIG. 3 is a schematic view of the overall structure of the high temperature heating assembly of the present utility model.

Fig. 4 is a schematic diagram of the internal structure of the first driving mechanism of the present utility model.

Fig. 5 is a schematic view of the overall structure of the right-side high-temperature heating assembly of the present utility model.

FIG. 6 is a schematic view of the entire structure of the low temperature heating assembly of the present utility model.

Fig. 7 is a schematic diagram of the overall structure of the driving mechanism of the present utility model.

Fig. 8 is a schematic diagram of a state of the microfluidic chip of the present utility model when it is positioned at a low temperature heating assembly for low temperature heating.

FIG. 9 is a schematic diagram showing the matching relationship between the sample heating mounting base and the second supporting base.

Reference numerals:

1001-low-temperature heating component, 101-left-side low-temperature heating component, 102-right-side low-temperature heating component, 103-second driving mechanism, 104-bearing, 105-low-temperature heating region, 106-motor fixing bracket, 107-sliding component, 108-first supporting seat, 109-sliding block, 110-sliding groove, 111-spring, 112-cylindrical pin, 113-bracket, 114-gear motor, 115-cam, 116-radiating block, 117-refrigerating plate, 118-heat conducting plate, 119-radiating fin, 120-radiating fan, 121-notch, 122-bearing bracket;

2001-high temperature heating assembly, 201-left high temperature heating assembly, 202-right high temperature heating assembly, 203-first driving mechanism, 204-high temperature heating area, 205-sample heating assembly, 206-return spring, 207-heating plate mount, 208-heating unit, 209-second support, 210-second spring, 211-second chute, 212-sample heating mount, 213-heating plate, 214-sliding protrusion, 215-sliding groove, 216-ceramic heating plate, 217-heat transfer plate, 218-guide hole, 219-guide rod, 220-first cam, 221-first gear motor, 222-mounting cylinder, 223-left housing, 224-right housing, 225-rotary cylinder;

3001-microfluidic chip;

4001-tester floor.

Detailed Description

Hereinafter, only certain exemplary embodiments are briefly described. As will be recognized by those of skill in the pertinent art, the described embodiments may be modified in numerous different ways without departing from the spirit or scope of the embodiments of the present utility model. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive.

In the description of the embodiments of the present utility model, it should be understood that the terms "length," "vertical," "horizontal," "top," "bottom," and the like indicate an orientation or a positional relationship based on that shown in the drawings, and are merely for convenience in describing the embodiments of the present utility model and to simplify the description, rather than to indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the embodiments of the present utility model.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the embodiments of the present utility model, the meaning of "plurality" is two or more, unless explicitly defined otherwise.

In the embodiments of the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured" and the like are to be construed broadly and include, for example, either permanently connected, removably connected, or integrally formed; the device can be mechanically connected, electrically connected and communicated; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the embodiments of the present utility model will be understood by those of ordinary skill in the art according to specific circumstances.

In embodiments of the utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, or may include both the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is less level than the second feature.

The following disclosure provides many different implementations, or examples, for implementing different configurations of embodiments of the utility model. In order to simplify the disclosure of embodiments of the present utility model, components and arrangements of specific examples are described below. Of course, they are merely examples and are not intended to limit embodiments of the present utility model. Furthermore, embodiments of the present utility model may repeat reference numerals and/or letters in the various examples, which are for the purpose of brevity and clarity, and which do not themselves indicate the relationship between the various embodiments and/or arrangements discussed.

Embodiments of the present utility model will be described in detail below with reference to the accompanying drawings.

Example 1

Referring to fig. 1-4, the present embodiment discloses a high temperature heating assembly, which includes a left side high temperature heating assembly 201, a right side high temperature heating assembly 202 and a first driving mechanism 203, wherein the left side high temperature heating assembly 201 is fixedly arranged on a bottom board 4001 of the tester, a high temperature heating area 204 is formed between the left side high temperature heating assembly and the right side high temperature heating assembly, the right side high temperature heating assembly 202 is slidably arranged on the bottom board 4001 of the tester, and the first driving mechanism 203 is used for driving the right side high temperature heating assembly 202 to approach or separate from the microfluidic chip 3001;

the right side high temperature heating component 202 is also provided with a sample heating component 205 in a sliding manner, the sample heating component 205 is arranged at the upper end of the right side high temperature heating component 202 in a sliding manner, and a reset spring 206 is arranged between the sample heating component 205 and the right side high temperature heating component 202.

In practical use, after the microfluidic chip 3001 moves into the high-temperature heating area 204, the high-temperature heating assembly 202 on the right side is close to the microfluidic chip 3001 under the action of the first driving mechanism 203 and contacts with the microfluidic chip 3001, and at this time, direct heating of the microfluidic chip 3001 can be achieved; meanwhile, the sample heating component 205 can also preheat the sample in the sample cavity of the microfluidic chip 3001; the heating efficiency of the micro-fluidic chip 3001 can be effectively improved;

wherein, the left and right high temperature heating components comprise a heating plate mounting seat 207 and a heating unit 208 fixedly arranged on the heating plate mounting seat 207,

the heating plate mount 207 in the left side high temperature heating assembly 201 is fixedly disposed on the tester base plate 4001;

the right side high temperature heating assembly 202 further comprises a second supporting seat 209 and a second spring 210, the second supporting seat 209 is fixedly arranged on the bottom plate 4001 of the tester, the heating plate mounting seat 207 in the right side high temperature heating assembly 202 is slidably arranged on the second supporting seat 209, two ends of the second spring 210 are respectively connected with the second supporting seat 209 and the heating plate mounting seat 207 in the right side high temperature heating assembly 202, and under the action of the second spring 210, the heating plate mounting seat 207 in the right side high temperature heating assembly 202 is contacted with the first driving mechanism 203.

In this way, in actual use, the heating plate mounting seat 207 can move on the second supporting seat 209, and the first driving mechanism 203 drives the heating plate mounting seat 207 to approach the microfluidic chip 3001, so that the heating unit 208 can approach the microfluidic chip 3001 and make contact, thereby improving heating efficiency; when resetting, under the action of the second spring 210, the heating plate mount 207 can return to make the heating unit 208 far away from the microfluidic chip 3001.

Further preferably, the second supporting seat 209 is provided with a second sliding groove 211, the heating plate mounting seat 207 in the right high-temperature heating assembly 202 is slidably arranged in the second sliding groove 211 through a sliding part, and the second spring 210 is positioned in the second sliding groove 211.

The second sliding groove 211 is provided to enable the right side high temperature heating assembly 202 to smoothly slide in the heating plate mount 207 in the right side high temperature heating assembly 202; in actual use, the second sliding groove 211 may be a dovetail type sliding groove or a T-type sliding groove.

Wherein, sample heating assembly 205 includes sample heating mount 211 and hot plate 213, and hot plate 213 is installed on sample heating mount 211, and sample heating mount 211 realizes sliding connection through the sliding protrusion 214 that sets up in sample heating mount 211 bottom and the sliding groove 215 that sets up on the hot plate mount 207 in right side high temperature heating assembly 202, return spring 206 is located in sliding groove 215.

So make sample heating mount pad 211 can be in smooth slip in sliding tray 215 to mainly play the purpose of buffering at reset spring 206, be provided with stop screw at the opening part of sliding tray 215, under reset spring 206's effect, sample heating mount pad 211 and stop screw contact, after the hot plate 213 that sets up on sample heating mount pad 211 contacted with micro-fluidic chip 3001, under reset spring 206's effect, can make hot plate 213 and micro-fluidic chip 3001 in close contact realization to the preheating of sample chamber.

The heating unit 208 includes a ceramic heating plate 216 and a heat transfer plate 217 attached to the ceramic heating plate 216, and the ceramic heating plate 216 is fixedly disposed on the heating plate mount 207.

In this way, the ceramic heating sheet 216 heats up, and the heating rate is high, and the heat is conducted through the heat transfer plate 217.

Further preferably, the heater plate mount 207 in the left side heater assembly 201 is provided with guide holes 218, and the heater plate mount 207 in the right side heater assembly 202 is provided with guide rods 219, and the guide rods 219 are matched with the guide holes 218.

The guide holes 218 and the guide rods 219 enable the heater plate mount 207 in the right side high temperature heating assembly 202 to be more stable when moving.

The first driving mechanism 203 includes a first cam 220, a mounting cylinder 222 for being fixedly mounted on the bottom plate 4001 of the tester, and a first gear motor 221 fixedly disposed on the mounting cylinder 222, wherein an output shaft of the first gear motor 221 extends into the mounting cylinder 222 and then is connected with the first cam 220, and the first cam 220 is in contact with the heating plate mounting seat 207 in the right high-temperature heating assembly 202.

The first cam 220 is driven to rotate by the first gear motor 221 provided, and the heating plate mount 207 in the right-side high-temperature heating assembly 202 is driven to move while the first cam 220 rotates.

In actual use, the mounting cylinder 222 comprises a left casing 223 and a right casing 224, the left casing 223 and the right casing 224 are connected by bolts to form an integrated structure, the first gear motor 221 is mounted on the right casing, and a yielding through groove is formed in the left casing 223.

Further preferably, a rotary drum 225 is rotatably disposed on the heater plate mount 207 in the right side high temperature heating assembly 202, and the side of the rotary drum 225 is in contact with the side of the first cam 220. This avoids the first cam 220 coming into direct contact with the heater plate mount 207 in the right side high temperature heating assembly 202, and reduces wear of the first cam 220 and the heater plate mount 207 in the right side high temperature heating assembly 202 by changing sliding friction to rolling friction through the drum 225.

In practical use, the output shaft of the first gear motor 221 passes through the first cam 220 and is rotatably connected with the right housing 224 through a gear or a shaft sleeve.

Example two

Referring to fig. 1-9, the present embodiment discloses a dual heating assembly of a real-time fluorescence PCR analyzer, comprising a high temperature heating assembly 2001 and a low temperature heating assembly 1001 for mounting on a bottom plate 4001 of the analyzer, wherein the high temperature heating assembly 2001 is used for heating a microfluidic chip 3001 at a high temperature, and the low temperature heating assembly 1001 is used for heating the microfluidic chip 3001 at a low temperature;

the cryoheating assembly 1001 includes a left cryoheating assembly 101, a right cryoheating assembly 102, and a second driving mechanism 103, where the left cryoheating assembly 101 is configured to be fixedly mounted on the tester base plate 4001, the right cryoheating assembly 102 is configured to be slidably mounted on the tester base plate 4001, and a cryoheating area 105 is formed between the left cryoheating assembly 101 and the right cryoheating assembly 102; the second driving mechanism 103 is used for driving the right low-temperature heating component 102 to approach or depart from the left low-temperature heating component 101, and the high-temperature heating component 2001 is one of the high-temperature heating components 2001 in the embodiment.

The embodiment is used for realizing the rapid switching of high-temperature heating and low-temperature heating of the micro-fluidic chip 3001, avoids the switching of high temperature and low temperature at one station, can effectively prolong the service life of a heating component, and simultaneously, the high-temperature heating and the low-temperature heating are separately carried out, so that the high-temperature heating component 2001 is always at a higher temperature, the low-temperature heating component 1001 is always at a temperature of intersection, the time of high-temperature and low-temperature switching of the same heating device is shortened, and the heating time is effectively shortened, and the detection efficiency is improved.

In this embodiment, referring to fig. 6, 7 and 8,

the left low-temperature heating component 101 and the right low-temperature heating component 102 are oppositely arranged, the second driving mechanism 103 is located at the tester bottom plate 4001 close to one side of the right low-temperature heating component 102, the second driving mechanism 103 is used for driving the right low-temperature heating component 102 to be close to or far away from the left low-temperature heating component 101, and the right low-temperature heating component 102 is used for conducting heat to the micro-fluidic chip 3001 after being contacted with the micro-fluidic chip 3001.

The utility model mainly comprises a left low-temperature heating component 101 and a right low-temperature heating component 102, wherein the left low-temperature heating component 101 is fixedly heated, the right low-temperature heating component 102 is movably heated (conducting heat and reducing temperature), in actual use, when a microfluidic chip 3001 moves into a low-temperature heating area 105, a second driving mechanism 103 drives the low-temperature heating component 1001 to move and contact the microfluidic chip 3001 after moving towards the direction of the microfluidic chip 3001, and at the moment, the right low-temperature heating component 102 can realize direct heat conduction to the microfluidic chip 3001, so that the heat conduction efficiency of the microfluidic chip 3001 can be effectively improved.

Wherein, right side low temperature heating element 102 is through slip subassembly 107 slidable mounting on tester bottom plate 4001, and slip subassembly 107 includes first supporting seat 108 and slider 109, is provided with spout 110 on the first supporting seat 108, and slider 109 slides and sets up in spout 110, and right side low temperature heating element 102 fixed mounting is provided with spring 111 on slider 109 in spout 110, under the effect of spring 111, right side low temperature heating element 102 and second actuating mechanism 103 contact.

Further preferably, a cylindrical pin 112 is vertically arranged on the sliding block 109, and the side surface of the cylindrical pin 112 is in contact with the second driving mechanism 103.

Further preferably, the sliding groove 110 is a T-shaped or dovetail-shaped sliding groove, and the sliding block 109 is provided with a sliding part matched with the sliding groove 110 in shape.

In the present embodiment, the sliding groove 110 is a T-shaped sliding groove, and the sliding portion is a T-shaped structure.

The second driving mechanism 103 comprises a bracket 113, a gear motor 114 and a cam 115, wherein the bracket 113 is used for being installed on a bottom plate 4001 of the tester, the gear motor 114 is fixedly installed on the bracket 113, the cam 115 is fixedly connected with the output end of the gear motor 114, and the cam 115 is contacted with the side face of the cylindrical pin 112.

The cam 115 is driven to rotate by the set gear motor 114, and as the first supporting seat 108 is slidably provided with the sliding block 109, under the action of the spring 111, the cylindrical pin 112 arranged on the sliding block 109 contacts with the side surface of the cam 115, and when the cam 115 rotates, the cam 115 drives the cylindrical pin 112 to move, so that the right low-temperature heating assembly 102 is stably driven.

Wherein, left side low temperature heating element 101 and right side low temperature heating element 102 all include radiating block 116, refrigeration piece 117 and heat conduction board 118, are provided with the mounting groove on the radiating block 116, refrigeration piece 117 fixed mounting is in the mounting groove, heat conduction board 118 is connected with refrigeration piece 117, the radiating block 116 in the left side low temperature heating element 101 is fixed on tester bottom plate 4001, right side low temperature heating element 102 is fixed on slider 109, the position of installing refrigeration piece 117 corresponds each other in left side low temperature heating element 101 and the right side low temperature heating element 102.

It should be noted that the low-temperature heating component is essentially a cooling device for cooling the heated microfluidic chip 3001, and the low-temperature heating component 1001 is referred to as a low-temperature heating component because the temperature in the low-temperature heating region 105 is greater than room temperature, and thus the low-temperature heating component is still in a heating state for the ambient temperature.

Most of PCR equipment in the market at present realizes the switching between heating and cooling at the same station, namely, the fastest heating for 18 seconds and the fastest cooling for 18 seconds at a time; the utility model heats at high temperature and low temperature (the temperature of the low temperature heating is lower than that of the high temperature heating), the high temperature heating time is about 11 seconds, and the cooling time is about 10 seconds.

After the microfluidic chip 3001 is heated at a high temperature, the microfluidic chip 3001 is moved into the low-temperature heating area 105, and at this time, the temperature of the low-temperature heating area 105 is far less than the temperature of the microfluidic chip 3001, so that the temperature reduction operation of the microfluidic chip 3001 can be realized.

The cooling plate 117 is actually a peltier, the cold end of the cooling plate 117 contacts with the heat conducting plate 118, so as to realize heat transfer, and the heat end of the cooling plate 117 contacts with the heat dissipating block 116 to dissipate heat.

Further optimizing, a plurality of radiating fins 119 are arranged on the side face of the radiating block 116, a radiating area is formed between every two adjacent radiating fins 119, and a radiating fan 120 is arranged on the radiating block 116; the heat dissipation effect can be effectively improved.

In actual use, the fins on the heat sink 116 in the right side cryoheating assembly 102 are provided with notches 121, and the cylindrical pins 112 are located in the notches 121.

By placing the cylindrical pins 112 in the notches 121, the occupation of the cylindrical pins 112 can be reduced, and the overall volume of the device can be reduced.

Example III

The second embodiment is further optimized based on the second embodiment, in the second embodiment, the support 113 includes a bearing support 122 and a motor fixing support 106, the bearing support 122 is fixedly installed on the bottom board 4001 of the tester, the motor fixing support 106 is installed on the bearing support 122, after the gear motor 114 is installed on the motor fixing support 106, an output shaft of the gear motor 114 passes through the cam 115 and is rotationally connected with the bearing support 122.

The bearing bracket 122 is provided with a bearing 104, and an output shaft of the gear motor 114 is rotatably connected with the bearing 104.

The motor fixing support 106 and the bearing support 122 form a split structure, so that the cam 115 is more convenient to install, and meanwhile, the output shaft of the gear motor 114 is in rotary connection with the bearing support 122 through the bearing 104, so that the cam 115 is more stable in rotation.

Example IV

This embodiment is further optimized based on the second or third embodiment, in which the cylindrical pin 112 is rotatably sleeved with an outer sleeve. The overcoat is installed on cylindric lock 112 through first bearing 104 rotation, and then makes the overcoat can take place relative rotation with cylindric lock 112, when cam 115 and overcoat contact, and cam 115 and overcoat realize sliding contact, when having avoided cam 115 and cylindric lock 112 contact, cause the wearing and tearing of cam 115 and cylindric lock 112, can make right side low temperature heating element 102 be close to micro-fluidic chip 3001 as far as like this and laminating, avoid because of cam 115, cylindric lock 112 wearing and tearing, cause right side low temperature heating element 102 and micro-fluidic chip 3001's clearance increase and influence heat conduction efficiency.

Further preferably, the end of the sliding groove 110 is provided with a guiding inclined plane, so that the sliding block 109 is more convenient to install.

While preferred embodiments of the present utility model have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the utility model.

The foregoing description of the preferred embodiment of the utility model is not intended to be limiting, but rather to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the utility model.

Claims (9)

1. A high temperature heating assembly, characterized by: the device comprises a left high-temperature heating component, a right high-temperature heating component and a first driving mechanism, wherein the left high-temperature heating component is fixedly arranged on a bottom plate of the tester, a high-temperature heating area is formed between the left high-temperature heating component and the right high-temperature heating component, the right high-temperature heating component is arranged on the bottom plate of the tester in a sliding manner, and the first driving mechanism is used for driving the right high-temperature heating component to be close to or far away from the microfluidic chip;

the right side high temperature heating assembly is provided with a sample heating assembly in a sliding mode, the sample heating assembly is arranged at the upper end of the right side high temperature heating assembly in a sliding mode, and a reset spring is arranged between the sample heating assembly and the right side high temperature heating assembly.

2. A high temperature heating assembly as defined in claim 1, wherein: the left and right side high temperature heating components comprise a heating plate mounting seat and a heating unit fixedly arranged on the heating plate mounting seat,

the heating plate mounting seat in the left high-temperature heating assembly is fixedly arranged on the bottom plate of the tester;

the right side high temperature heating component further comprises a second supporting seat and a second spring, the second supporting seat is fixedly arranged on the bottom plate of the tester, the heating plate installation seat in the right side high temperature heating component is slidably installed on the second supporting seat, two ends of the second spring are respectively connected with the second supporting seat and the heating plate installation seat in the right side high temperature heating component, and under the action of the second spring, the heating plate installation seat in the right side high temperature heating component is contacted with the first driving mechanism.

3. A high temperature heating assembly as defined in claim 2, wherein: the second supporting seat is provided with a second chute, the heating plate installation seat in the right high-temperature heating assembly is arranged in the second chute in a sliding way through the sliding part, and the second spring is positioned in the second chute.

4. A high temperature heating assembly as defined in claim 2, wherein: the sample heating assembly comprises a sample heating installation seat and a heating plate, the heating plate is installed on the sample heating installation seat, the sample heating installation seat is in sliding connection with a sliding groove on the heating plate installation seat arranged on the right side through a sliding protrusion arranged at the bottom of the sample heating installation seat, and the reset spring is located in the sliding groove.

5. A high temperature heating assembly as defined in claim 2, wherein: the heating unit comprises a ceramic heating plate and a heat transfer plate attached to the ceramic heating plate, and the ceramic heating plate is fixedly arranged on the heating plate mounting seat.

6. A high temperature heating assembly as defined in claim 2, wherein: the heating plate mounting seat in the left high-temperature heating component is provided with a guide hole, and the heating plate mounting seat in the right high-temperature heating component is provided with a guide rod which is matched with the guide hole.

7. A high temperature heating assembly as defined in claim 2, wherein: the first driving mechanism comprises a first cam, a mounting cylinder fixedly mounted on the bottom plate of the tester and a first gear motor fixedly arranged on the mounting cylinder, an output shaft of the first gear motor extends into the mounting cylinder and then is connected with the first cam, and the first cam is in contact with a heating plate mounting seat in the right high-temperature heating assembly.

8. A high temperature heating assembly as defined in claim 7, wherein: the rotary drum is rotatably arranged on the heating plate mounting seat in the right high-temperature heating assembly, and the side surface of the rotary drum is contacted with the side surface of the first cam.

9. A dual heating assembly for a real-time fluorescent PCR analyzer, characterized by: the device comprises a high-temperature heating component and a low-temperature heating component which are arranged on a bottom plate of the tester, wherein the high-temperature heating component is used for heating the microfluidic chip at high temperature, and the low-temperature heating component is used for heating the microfluidic chip at low temperature;

the low-temperature heating assembly comprises a left low-temperature heating assembly, a right low-temperature heating assembly and a second driving mechanism, wherein the left low-temperature heating assembly is fixedly arranged on the bottom plate of the tester, the right low-temperature heating assembly is slidably arranged on the bottom plate of the tester, and a low-temperature heating area is formed between the left low-temperature heating assembly and the right low-temperature heating assembly; the second driving mechanism is used for driving the right low-temperature heating component to be close to or far away from the left low-temperature heating component, and the high-temperature heating component is a high-temperature heating component according to any one of claims 1-8.