CN219590204U - Fungal spore suspension counter - Google Patents

Abstract

The utility model discloses a fungal spore suspension counter, wherein one end of a cantilever is arranged on a base of the counter; a microscope camera, an objective lens interface and an objective lens are sequentially and downwards arranged on the lower end surface of the other end of the cantilever; the blood cell counting plate is arranged on the device base through a blood cell counting plate bracket and is positioned between the device base and the objective lens; an adjustable cold light source is arranged on a device base below the blood cell counting plate; a plurality of device supporting legs are arranged below the device base to support the whole device; the device is simple to operate, has extremely low requirements on personnel, provides great convenience for spore concentration quantification of fungus spore suspension, greatly saves time and energy wasted by test personnel in spore counting, and has more accurate spore concentration quantification result.

Description

Fungal spore suspension counter

Technical Field

The utility model belongs to the field of microorganism detection, and particularly relates to a fungal spore suspension counter.

Background

In microbiological examination and detection tests in the fields of medicines, medical instruments and the like, quantitative counting of spore concentration in prepared fungal spore suspension is often required. Common methods for counting fungal spore suspensions include plate count and plate count, which are not commonly used because they require 5-7 days of incubation to obtain the count, and are time consuming and laborious. The hemocytometer is a method in which a volume of fungal spore suspension is dripped onto a hemocytometer covered with a cover slip, amplified by an optical microscope, and identified and counted by human eyes. However, this method has the following disadvantages: 1. the ocular lens of the optical microscope is smaller, and can cause eye fatigue after long-time use; 2. the multiple of the objective lens, the height of the objective table and the visual field are required to be adjusted every time the optical microscope is used, and the requirements on personnel are high; 3. error is easy to cause and time is consumed; 4. no special equipment is needed, and the optical microscope adopted at present has high cost and is not smart and convenient enough. Therefore, in the prospect of rapid development of modern science and technology, it is very necessary to develop a set of smart and convenient instruments dedicated to spore counting in fungal spore suspensions.

Disclosure of Invention

The utility model aims at overcoming the defects of the prior art and provides a fungal spore suspension counter.

The aim of the utility model is realized by the following technical scheme: a fungal spore suspension counter, the counter comprising: the device comprises a device cantilever, a microscope camera, an objective lens interface, an objective lens, a blood cell counting plate bracket, a cold light source, a device base and background processing equipment;

the main rod of the device cantilever is arranged on the device base; a microscope camera, an objective lens interface and an objective lens are sequentially and downwards arranged on the lower end surface of the cantilever cross rod; the blood cell counting plate is arranged on the device base through a blood cell counting plate bracket and is positioned between the device base and the objective lens; the cold light source is arranged on the device base in the middle of the blood cell counting plate bracket, and the background processing equipment is connected with the microscope camera in a wireless way and receives pictures shot by the microscope camera for storage and viewing.

Further, the microscope camera has a function of transmitting pictures through WIFI or Bluetooth.

Further, the dust cap is transparent; the dust cover is mounted on the device base and covers all devices except the background processing equipment.

Further, the objective lens is an optical microscope lens, and the magnification is 400 times.

Further, a mobile power supply is arranged on the bottom surface of the device base; providing needed power supply for the cold light source and the microscope camera through the circuit; a brightness adjusting knob is arranged between the cold light source and the mobile power supply; for adjusting the brightness of the light source.

Further, the cantilever main rod is a telescopic rod regulated by a bolt.

Further, the background processing device comprises a computer or a mobile phone capable of storing and amplifying images.

The utility model has the beneficial effects that:

the images in the visual field are transmitted to a computer or mobile phone terminal in a wireless mode, so that the images can be amplified and stored, and the counting result can be traced back; meanwhile, spores are counted on the screen, so that eye fatigue caused by long-time use of the microscope is avoided, long-time occupation of the microscope is avoided, and the utilization rate of the instrument is increased. The device is simple to operate, has extremely low requirements on personnel, provides great convenience for spore concentration quantification of fungus spore suspension, greatly saves time and energy wasted by test personnel in spore counting, and has more accurate spore concentration quantification result.

Drawings

FIG. 1 is a schematic diagram of a fungal spore suspension counter device according to an embodiment of the present utility model;

in the figure: 1. a dust cover; 2. a device cantilever; 3. a microscope camera; 4. an objective interface; 5. an optical microscope lens; 6. a blood cell counting plate; 7. a blood cell counting plate holder; 8. a cold light source; 9. an adjustment button; 10. a mobile power supply; 11. a device leg; 12. and a device base.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments. All other embodiments, based on a single embodiment of the utility model, which would be apparent to one of ordinary skill in the art without making any inventive effort are intended to be within the scope of the utility model.

As shown in fig. 1, the utility model provides a fungal spore suspension counter,

one end of the device cantilever 2 is arranged on the device base 10; a microscope camera 3, an objective lens interface 4 and an objective lens 5 are sequentially arranged downwards on the lower end surface of the other end of the cantilever 2; a blood cell counting plate bracket 7 through which the blood cell counting plate 6 passes is arranged on the device base 10 and is positioned between the device base 10 and the objective lens 5; an adjustable cold light source 8 is mounted on the device base 10 below the blood cell count plate 6; a plurality of device supporting legs 11 are arranged below the device base 10 to support the whole device; a mobile power supply 10 is arranged on the bottom surface of the device base; the cold light source 8 and the microscope camera 3 are provided with required power sources through lines; a brightness adjusting knob 9 is arranged between the cold light source 8 and the mobile power supply 10; for adjusting the brightness of the light source; the background processing equipment is used for receiving the pictures transmitted by the microscope camera 3 for storage and viewing; the dust cover 1 is mounted on the device base 10, and covers all devices except for the background processing equipment.

The device is divided into two parts, one part is a device body, the other part is background processing equipment, the device is shown as a main body structure diagram, the dust cover 1 in the device is made of transparent acrylic, and the device is protected from being polluted by external dust and from being damaged by external force in a test interval period; the cantilever 2 of the device plays a role in installing a microscope lens and a microscope camera; the model 3 of the microscope camera is a Murzider M2000 type high-definition digital camera, and the image signal can be transmitted to a computer/mobile phone end through wifi; the objective lens interface 4 plays a role of connecting the camera and the objective lens; the optical microscope 5 can be amplified by about 400 times according to actual needs; the blood cell counting plate 6 carries a fungal spore suspension to be observed, and is covered with a cover glass; the blood cell counting plate support 7 plays a role of supporting a blood cell counting plate; the model of the cold light source 8 is Rainbow vision JH-RS502660-W annular cold light source, and the required brightness can be obtained through the adjusting button 9; the mobile power supply 10 provides a 12V power supply for a cold light source and a microscope camera through a circuit; the device legs 11 and the device base 12 support the whole set of device;

the second part is a background processing computer which is used for presenting the uploaded image in real time, so that the laboratory staff can conveniently identify and count spores in the image, and meanwhile, the image and the counting result can be summarized and archived together.

Example 1: first, a blood cell counting plate (25×16 or 16×25) is covered with a cover glass. And taking fungus spore suspension by using a pipette, dripping the fungus spore suspension on the edge of a cover glass, and slowly infiltrating the spore suspension into a counting chamber to avoid generating bubbles. Next, the blood cell count plate 6 is placed on the blood cell count plate holder 7. The mobile power supply 10 is turned on, WIFI is connected, and the image is transmitted to a mobile phone or a computer. The brightness of the cold light source 8 is regulated by the regulating button 9, and meanwhile, the length of the main rod of the cantilever 2 of the device is regulated, so that the brightness of the image is moderate and the picture is clear. Finally, by moving the position of the blood cell counting plate 6, the middle square on the counting plate is found, spores are identified, counted and counted.

The calculation method is that

(1) Blood cell counting plate gaugeWhen the grid is 25×16, the total number of spores N in 5 middle grids is counted, and then the final concentration is calculated according to a formula: (N/5). Times.25.times.10 ⁴ cfu/ml；

(2) When the specification of the hemocytometer is 16×25 grids, the total number of spores N of 4 middle grids is counted, and then the final concentration is calculated according to a formula: (N/4). Times.15.times.10 ⁴ cfu/ml。

The above-described embodiments are intended to illustrate the present utility model, not to limit it, and any modifications and variations made thereto are within the spirit of the utility model and the scope of the appended claims.

Claims (7)

1. A fungal spore suspension counter, the counter comprising: the device comprises a device cantilever (2), a microscope camera (3), an objective lens interface (4), an objective lens (5), a blood cell counting plate (6), a blood cell counting plate bracket (7), a cold light source (8), a device base (12) and background processing equipment;

the main rod of the device cantilever (2) is arranged on the device base (12); a microscope camera (3), an objective lens interface (4) and an objective lens (5) are sequentially and downwards arranged on the lower end surface of the cross rod of the cantilever (2); the blood cell counting plate (6) is arranged on the device base (12) through the blood cell counting plate bracket (7) and is positioned between the device base (12) and the objective lens (5); the cold light source (8) is arranged on a device base (12) in the middle of the blood cell counting plate bracket (7), and the background processing equipment is connected with the microscope camera (3) in a wireless way and receives images shot by the microscope camera (3) for storage and viewing.

2. A fungal spore suspension counter according to claim 1, characterised in that the microscope camera (3) has the function of transmitting images via WIFI or bluetooth.

3. A fungal spore suspension counter according to claim 1, characterised in that the fungal spore suspension counter further comprises a dust cover (1), the dust cover (1) being transparent; the dust cover (1) is mounted on the device base (12) to cover all devices except for the background processing equipment.

4. A fungal spore suspension counter according to claim 1, characterised in that the objective (5) is an optical microscope lens with a magnification of 400.

5. A fungal spore suspension counter according to claim 1, characterised in that the device base (12) has a mobile power supply (10) mounted on its underside; the cold light source (8) and the microscope camera (3) are provided with required power supplies through lines; a brightness adjusting knob (9) is arranged between the cold light source (8) and the mobile power supply (10); for adjusting the brightness of the light source.

6. A fungal spore suspension counter according to claim 1, wherein the cantilever boom is a bolt-adjustable telescopic boom.

7. A fungal spore suspension counter according to claim 1, wherein the background processing device comprises a computer or a cell phone capable of storing and magnifying images.