CN219526600U

CN219526600U - Real-time fluorescence quantitative PCR appearance sampling device

Info

Publication number: CN219526600U
Application number: CN202320685227.9U
Authority: CN
Inventors: 陈晨
Original assignee: Feisite Hebei Biotechnology Co ltd
Current assignee: Feisite Hebei Biotechnology Co ltd
Priority date: 2023-03-31
Filing date: 2023-03-31
Publication date: 2023-08-15
Anticipated expiration: 2033-03-31

Abstract

The utility model belongs to the field of sample injection equipment, in particular to a real-time fluorescence quantitative PCR instrument sample injection device, which comprises a feeding frame, wherein a control box is fixedly arranged on the rear side of the feeding frame, moving grooves are formed in the inner walls of the two sides of the feeding frame, a moving plate is slidably arranged in the moving grooves, the front sides of the two moving plates are fixedly provided with the same upper carriage, a plurality of tube placing holes are formed in the upper carriage, the bottom of the upper carriage is slidably provided with a lower fixed plate, the lower fixed plate is provided with a fixed hole, the fixed hole is matched with the tube placing holes, the same threaded rod is rotatably arranged on the inner walls of the front side and the rear side of the moving groove, the moving plate is sleeved on the threaded rod in a threaded manner, one end of the threaded rod is compressed in the control box, and one side of the control box is provided with a power component. The utility model has reasonable design, the purpose of sampling the sample tube can be realized by moving the upper carriage, and the purpose of clamping and fixing the sample tube can be realized by the tube placing hole and the fixing hole.

Description

Real-time fluorescence quantitative PCR appearance sampling device

Technical Field

The utility model relates to the technical field of sample injection equipment, in particular to a sample injection device of a real-time fluorescent quantitative PCR instrument.

Background

The real-time fluorescence quantitative pcr instrument consists of a fluorescence quantitative system and a computer, wherein the fluorescence quantitative system is used for monitoring fluorescence in a circulation process, the computer connected with real-time equipment is used for collecting fluorescence data, the data is displayed in a chart form through developed real-time analysis software, raw data are drawn into a chart of fluorescence intensity relative to the circulation number, analysis can be started after the raw data are collected, the software of the real-time equipment can enable the collected data to be subjected to normalization processing to make up for differences of background fluorescence, and threshold level can be set after normalization, namely the level of analysis fluorescence data.

When the real-time fluorescence quantitative PCR instrument is used, a sample is firstly required to be imported, a PCR reaction system is generally manually added and sequentially put into heating holes of the instrument, but the temperature of the heating holes is higher, and scalding is easy to occur, so that the real-time fluorescence quantitative PCR instrument sampling device is provided for solving the problems.

Disclosure of Invention

The utility model aims to solve the defects in the prior art, and provides a real-time fluorescence quantitative PCR instrument sample injection device.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the real-time fluorescence quantitative PCR instrument sample injection device comprises a feeding frame, wherein a control box is fixedly arranged at the rear side of the feeding frame, moving grooves are formed in the inner walls of two sides of the feeding frame, moving plates are slidably arranged in the moving grooves, the front sides of the two moving plates are fixedly provided with the same upper carriage, a plurality of tube placing holes are formed in the upper carriage, a lower fixing plate is slidably arranged at the bottom of the upper carriage, fixing holes are formed in the lower fixing plate, and the fixing holes are matched with the tube placing holes;

the front side and the rear side inner wall of the movable groove are rotatably provided with the same threaded rod, the movable plate is sleeved on the threaded rod in a threaded mode, one end of the threaded rod is compressed in the control box, one side of the control box is provided with a power assembly, and the power assembly is connected with the two threaded rods.

Preferably, the power assembly comprises a driving mechanism and a transmission mechanism, the driving mechanism is arranged on the control box, and the transmission mechanism is arranged between the driving mechanism and the threaded rod.

Preferably, the driving mechanism comprises a driving motor and a driving shaft, the driving shaft is rotatably arranged on the inner walls of the two sides of the control box, the driving motor is fixedly arranged on one side of the control box, and an output shaft of the driving motor is fixedly connected with the driving shaft.

Preferably, the transmission mechanism comprises a driving bevel gear and a driven bevel gear, wherein the driving bevel gear is fixedly arranged on the driving shaft, the driven bevel gear is fixedly arranged on the threaded rod, and the driving bevel gear is meshed with the corresponding driven bevel gear.

Preferably, the bottom of the upper carriage is fixedly provided with a partition board, one side of the lower fixed plate is fixedly provided with an extrusion rod, the extrusion rod penetrates through the partition board and is matched with the feeding frame, and a reset mechanism is arranged between the extrusion rod and the partition board.

Preferably, the reset mechanism comprises a baffle and a reset spring, the baffle is fixedly arranged on the extrusion rod, and the reset spring is arranged between the baffle and the baffle.

Preferably, a connecting seat is fixedly arranged at the bottom of the upper carriage, a guide groove is formed in one side of the connecting seat, a guide rod is slidably arranged in the guide groove, and the guide rod is fixedly connected with the lower fixing plate.

Preferably, the limiting grooves are formed in the inner wall of the top and the inner wall of the bottom of the moving groove, limiting seats are fixedly arranged at the top and the bottom of the moving plate, and the limiting seats are in sliding connection with the corresponding limiting grooves.

The utility model has the beneficial effects that:

1. the sample tube is sequentially placed in the tube placing holes of the upper pushing plate, the driving motor is started to drive the driving shaft to rotate, the driving shaft can drive the two threaded rods to rotate through the cooperation of the driving bevel gear and the driven bevel gear, and the threaded rods can drive the moving plate and the upper supporting plate to move towards the feeding frame, so that the sample tube sample injection purpose can be realized;

2. when the upper carriage moves, the upper supporting plate can drive the lower fixing plate to move, when the extrusion rod is in contact with the sample injection frame, when the upper supporting plate continues to move, the upper supporting plate and the lower fixing plate move relatively, and at the moment, the tube placing holes and the fixing holes are staggered, so that the purpose of clamping and fixing the sample tube can be achieved through the tube placing holes and the fixing holes.

Drawings

FIG. 1 is a schematic diagram of a real-time fluorescence quantitative PCR instrument sample injection device in a three-dimensional structure;

FIG. 2 is a schematic diagram of a sample injection device of a real-time fluorescent quantitative PCR apparatus according to the present utility model;

FIG. 3 is a schematic diagram of a portion A of a sample injection device of a real-time fluorescence quantitative PCR instrument according to the present utility model;

FIG. 4 is a schematic diagram of a sample injection device of a real-time fluorescent quantitative PCR apparatus according to the present utility model;

fig. 5 is a schematic diagram of a part B of a sample injection device of a real-time fluorescent quantitative PCR instrument according to the present utility model.

In the figure: 1. a feeding frame; 2. a control box; 3. an upper carriage; 4. a pipe hole is arranged; 5. a moving groove; 6. a moving plate; 7. a threaded rod; 8. a return spring; 9. a driven bevel gear; 10. a driving motor; 11. a drive shaft; 12. a drive bevel gear; 13. a fixing hole; 14. a lower fixing plate; 15. a partition plate; 16. an extrusion rod; 17. and a baffle.

Detailed Description

The technical scheme of the present utility model will be clearly and completely described in connection with specific embodiments. It will be apparent that the described embodiments are only some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to fall within the scope of the utility model.

When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present, the term "disposed" indicating a manner of presence and may be connected, mounted, fixedly connected, operatively connected, or the like. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. The terminology used herein in the description of the utility model is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1-5, a real-time fluorescence quantitative PCR instrument sampling device, including feeding frame 1, control box 2 is fixedly installed to the rear side of feeding frame 1, all offer on the both sides inner wall of feeding frame 1 and remove groove 5, sliding mounting has movable plate 6 in the removal groove 5, the front side fixed mounting of two movable plates 6 has same upper carriage 3, a plurality of hole 4 of putting has been offered on the upper carriage 3, lower fixed plate 14 is installed to the bottom slidable mounting of upper carriage 3, fixed orifices 13 has been offered on the lower fixed plate 14, and fixed orifices 13 and hole 4 looks adaptation put, the same threaded rod 7 is installed in the rotation on the front side and the rear side inner wall of movable groove 5, and movable plate 6 thread bush is established on threaded rod 7, in the one end compression control box 2 of threaded rod 7, one side of control box 2 is equipped with power component, and power component is connected with two threaded rods 7, more specifically, the spacing groove has been offered on the top inner wall of movable plate 6 and the bottom inner wall, and spacing seat has all fixed mounting, and spacing seat and corresponding spacing seat are connected with spacing seat, can realize moving plate 6 through setting up spacing seat and spacing seat, can realize moving 6.

In this embodiment, the power component includes actuating mechanism and drive mechanism, actuating mechanism installs on control box 2, and be equipped with drive mechanism between actuating mechanism and the threaded rod 7, actuating mechanism includes driving motor 10 and drive shaft 11, drive shaft 11 rotates and installs on the both sides inner wall of control box 2, driving motor 10 fixed mounting is in one side of control box 2, driving motor 10's output shaft and drive shaft 11 fixed connection, through being provided with actuating mechanism, driving motor 10 can drive shaft 11 and rotate, above-mentioned drive mechanism includes drive bevel gear 12 and driven bevel gear 9, drive bevel gear 12 fixed mounting is on drive shaft 11, driven bevel gear 9 fixed mounting is on threaded rod 7, and drive bevel gear 12 meshes with corresponding driven bevel gear 9, under the cooperation of drive bevel gear 12 and driven bevel gear 9, drive shaft 11 can drive two threaded rods 7 and rotate.

In this embodiment, baffle 15 is fixed mounting in the bottom of last planker 3, one side fixed mounting of lower fixed plate 14 has extrusion pole 16, extrusion pole 16 runs through baffle 15 and with feeding frame 1 looks adaptation, be equipped with canceling release mechanical system between extrusion pole 16 and the baffle 15, canceling release mechanical system includes baffle 17 and return spring 8, baffle 17 fixed mounting is on extrusion pole 16, install return spring 8 between baffle 17 and the baffle 15, through being provided with return spring 8, return spring 8 can promote lower fixed plate 14 and reset, the bottom fixed mounting of upper planker 3 has the connecting seat, the guide way has been seted up to one side of connecting seat, slidable mounting has the guide bar in the guide way, and guide bar and lower fixed plate 14 fixed connection, under the cooperation of guide way and guide bar, can realize leading lower fixed plate 14 for lower fixed plate 14 can carry out stable removal.

According to the utility model, a sample tube is sequentially placed in the tube placing hole 4 of the upper push plate, the driving motor 10 is started, the driving motor 10 can drive the driving shaft 11 to rotate, the driving shaft 11 can drive the two threaded rods 7 to rotate under the cooperation of the driving bevel gear 12 and the driven bevel gear 9, the threaded rods 7 can drive the moving plate 6 and the upper support plate to move towards the feeding frame 1, so that the purpose of sample tube injection can be realized, when the upper support plate 3 moves, the upper support plate can drive the lower fixing plate 14 to move, when the extruding rod 16 is in contact with the injection frame, when the upper support plate moves continuously, the upper support plate moves relatively with the lower fixing plate 14, at this time, the tube placing hole 4 and the fixing hole 13 are dislocated, and the purpose of sample tube clamping and fixing can be realized through the tube placing hole 4 and the fixing hole 13.

The sample injection device of the real-time fluorescence quantitative PCR instrument provided by the utility model is described in detail above. The principles and embodiments of the present utility model have been described herein with reference to specific examples, which are intended to be merely illustrative of the methods of the present utility model and their core ideas. It should be noted that it will be apparent to those skilled in the art that various modifications and adaptations of the utility model can be made without departing from the principles of the utility model and these modifications and adaptations are intended to be within the scope of the utility model as defined in the following claims.

Claims

1. The utility model provides a real-time fluorescence ration PCR appearance sampling device, its characterized in that includes feeding frame (1), the rear side fixed mounting of feeding frame (1) has control box (2), all seted up on the both sides inner wall of feeding frame (1) and moved groove (5), move movable plate (6) are installed to movable groove (5) sliding, two the front side fixed mounting of movable plate (6) has same upper carriage (3), a plurality of hole (4) of putting have been seted up on upper carriage (3), the bottom slidable mounting of upper carriage (3) has lower fixed plate (14), set up fixed orifices (13) on lower fixed plate (14), just fixed orifices (13) and put hole (4) looks adaptation;

the inner walls of the front side and the rear side of the moving groove (5) are rotatably provided with the same threaded rod (7), the moving plate (6) is sleeved on the threaded rod (7) in a threaded way, one end of the threaded rod (7) is compressed in the control box (2), one side of the control box (2) is provided with a power assembly, and the power assembly is connected with the two threaded rods (7).

2. The sample injection device of the real-time fluorescence quantitative PCR instrument according to claim 1, wherein the power assembly comprises a driving mechanism and a transmission mechanism, the driving mechanism is installed on the control box (2), and the transmission mechanism is arranged between the driving mechanism and the threaded rod (7).

3. The real-time fluorescence quantitative PCR instrument sample injection device according to claim 2, wherein the driving mechanism comprises a driving motor (10) and a driving shaft (11), the driving shaft (11) is rotatably installed on the inner walls of the two sides of the control box (2), the driving motor (10) is fixedly installed on one side of the control box (2), and an output shaft of the driving motor (10) is fixedly connected with the driving shaft (11).

4. The real-time fluorescence quantitative PCR instrument sample feeding device according to claim 2, wherein the transmission mechanism comprises a driving bevel gear (12) and a driven bevel gear (9), the driving bevel gear (12) is fixedly arranged on a driving shaft (11), the driven bevel gear (9) is fixedly arranged on a threaded rod (7), and the driving bevel gear (12) is meshed with the corresponding driven bevel gear (9).

5. The real-time fluorescence quantitative PCR instrument sample injection device according to claim 1, wherein a partition plate (15) is fixedly arranged at the bottom of the upper carriage (3), an extrusion rod (16) is fixedly arranged at one side of the lower fixed plate (14), the extrusion rod (16) penetrates through the partition plate (15) and is matched with the feeding frame (1), and a reset mechanism is arranged between the extrusion rod (16) and the partition plate (15).

6. The real-time fluorescence quantitative PCR instrument sample injection device according to claim 5, wherein the reset mechanism comprises a baffle plate (17) and a reset spring (8), the baffle plate (17) is fixedly arranged on the extrusion rod (16), and the reset spring (8) is arranged between the baffle plate (17) and the partition plate (15).

7. The real-time fluorescence quantitative PCR instrument sample injection device according to claim 1, wherein a connecting seat is fixedly arranged at the bottom of the upper carriage (3), a guide groove is formed in one side of the connecting seat, a guide rod is slidably arranged in the guide groove, and the guide rod is fixedly connected with the lower fixing plate (14).

8. The sample injection device of the real-time fluorescence quantitative PCR instrument according to claim 1, wherein limit grooves are formed in the inner wall of the top and the inner wall of the bottom of the movable groove (5), limit seats are fixedly arranged at the top and the bottom of the movable plate (6), and the limit seats are in sliding connection with the corresponding limit grooves.