CN219455951U - Immunoassay instrument - Google Patents
Immunoassay instrument Download PDFInfo
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- CN219455951U CN219455951U CN202223288697.6U CN202223288697U CN219455951U CN 219455951 U CN219455951 U CN 219455951U CN 202223288697 U CN202223288697 U CN 202223288697U CN 219455951 U CN219455951 U CN 219455951U
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Abstract
The utility model discloses an immunoassay analyzer, which comprises a shell, wherein a standard curve acquisition module, a detection module, a printing module and a main control module are arranged in the shell; the standard curve acquisition module, the detection module and the printing module are respectively connected with the main control module in an information manner; the detection module comprises a reaction cup holder, a light measuring system and a driving system, wherein the driving system is connected with the reaction cup holder, and the light measuring system and the driving system are respectively in information connection with the main control module. The immunity analyzer can be applied to test heterogeneous phase test solution, converts color signals into digital signals, calculates concentration values and opens up a new field of application.
Description
Technical Field
The utility model belongs to the field of medical instruments, and particularly relates to an immunoassay instrument.
Background
The immunoassay instrument in the prior art mainly comprises a fluorescence immunoassay instrument, an enzyme-labeled instrument, a chemiluminescence immunoassay instrument and the like. The fluorescent immunoassay analyzer is mainly a test instrument for solid phase chromatography reaction, and mainly converts fluorescent signals into digital signals by collecting the fluorescent signals. The enzyme-labeled instrument is an instrument for liquid phase reaction test, is calculated by converting absorbance of a collected sample into a digital signal, and requires uniformity of a system for test. The chemiluminescent immunoassay analyzer is an immunoassay analyzer using chemiluminescent agent to directly label antigen or antibody, wherein a core detection device is a photomultiplier tube, the photomultiplier tube is used for single photon detection and transmission to an amplifier, high-voltage current is applied for amplification, the amplifier converts analog current into digital current, and the digital current transmits a luminescent signal to a computer for calculation, so that a clinical result is obtained.
These instruments are several types of immunoassays commonly used at present, but cannot directly test samples with different colors, and cannot obtain ideal test results.
The applicant has developed a magnetic particle immunoadsorption technical platform which is a liquid phase reaction system. The reagent mainly comprises a reagent A and a reagent B, wherein the reagent A contains magnetic particles, and antigens or antibodies can be coated on the magnetic particles; the reagent B contains non-magnetic color latex microspheres, and antigens or antibodies can be coated on the non-magnetic color latex microspheres. When the test solution reacts, the reagent A is added, then the sample to be tested is added, the substances in the sample to be tested can be combined with the antigens or the antibodies on the magnetic particles, then the reagent B is added, the antibodies or the antigens on the non-magnetic color latex microspheres in the reagent B are combined with the other sites of the substances to be tested, and finally the magnetic particle-antigen (antibody) -substance to be tested-latex microsphere compound is formed, the compound is precipitated along with the magnetic particles under the action of an external magnetic field, the latex microspheres which are not combined are left in the supernatant, and the color in the supernatant and the concentration of the substances to be tested are in a certain proportion relation.
Because the reagent has color change and the color can be set arbitrarily, the preferable color range is mainly between red and blue; and because of the presence of microspheres, the color change needs to be measured by an instrument, so that an instrument capable of being used for color measurement needs to be developed.
Disclosure of Invention
In order to solve the technical problems, the utility model provides an immunoassay analyzer, which comprises the following technical scheme: an immunity analyzer comprises a shell, wherein a standard curve acquisition module, a detection module, a printing module and a main control module are arranged in the shell; the standard curve acquisition module, the detection module and the printing module are respectively connected with the main control module in an information manner; the detection module comprises a reaction cup holder, a light measuring system and a driving system, wherein the driving system is connected with the reaction cup holder, and the light measuring system and the driving system are respectively in information connection with the main control module.
Preferably, the reaction cup holder is a reaction cup groove, and the reaction cup is placed in the reaction cup groove; the driving system drives the reaction cup holder to slide horizontally in the shell.
Preferably, the reaction cup is a colorless transparent multi-joint cup, and the reaction cup groove is made of dark opaque materials; the driving system drives the reaction cup holder to slide along the axial direction of the reaction cup holder.
Preferably, the light measuring system comprises a light source and a signal collector, wherein light rays of the light source are directly incident into the reaction cup, and the signal collector absorbs light rays refracted or reflected by the reaction cup.
Preferably, small holes are arranged on the side surfaces of the reaction cup grooves corresponding to the positions of each reaction cup, and the heights of the small holes are not higher than the height of the liquid level in the reaction cup; the light source directly irradiates into the reaction cup from the small hole, and the signal collector above the reaction cup absorbs the light signal refracted out from the reaction cup.
Preferably, the bottom of the reaction cup bottom is provided with a magnetic piece at a position corresponding to each reaction cup.
Preferably, the shell is also provided with a sample inlet, a display module and a scanning module, the sample inlet is used for placing the reaction cup into the reaction cup groove, the scanning module scans the graphic code on the sample package to be tested, the display module and the scanning module are respectively in information connection with the main control module, and the main control module comprises a microcontroller unit.
Preferably, the standard curve acquisition module comprises an IC card reader, the light source is an LED light source, the signal acquisition device comprises a receiving sensor, and the printing module comprises a thermal printer.
Preferably, the diameter of the small hole on the side surface of the reaction cup groove is 1mm, and the small hole is arranged at the middle of the side surface of the reaction cup groove corresponding to the middle lower part of the reaction cup.
Preferably, the reaction cups are six-way cups, the bottom of each reaction cup is a narrow plane, the rear side surface of each reaction cup is an inclined plane, and the rear side surface of each reaction cup groove is provided with a slope matched with the rear side surface of each reaction cup.
The beneficial effects of the utility model are as follows: the utility model relates to an immunoassay analyzer for liquid phase reaction reagents, which is different from the conventional immunoassay analyzer in that absorbance signals are converted into digital signals, can be applied to test heterogeneous phase test solutions, converts the color signals into digital signals, calculates concentration values, and initiates application in a new field.
Drawings
Fig. 1 is a front view of an embodiment of the present utility model.
Fig. 2 is a schematic structural view of an upper case according to an embodiment of the present utility model.
Fig. 3 is a schematic view of the structure of the lower case according to the embodiment of the present utility model.
Fig. 4 is an information connection diagram of each module according to an embodiment of the present utility model.
FIG. 5 is a schematic view of the structure of a reaction cup according to an embodiment of the present utility model.
Reference numerals: the device comprises a shell 1, a standard curve acquisition module 2, a detection module 3, a printing module 4, a main control module 5, a photometry system 6 and a signal acquisition device 61; the device comprises a driving system 7, a reaction cup 8, a reaction cup groove 9, a sample inlet 10, a display module 11, a scanning module 12, an upper shell 13 and a lower shell 14.
Detailed Description
The utility model is further described below with reference to the drawings and examples.
The present embodiment provides an immunoassay analyzer including a housing 1, the housing 1 including an upper case 13 and a lower case 14. The shell 1 is internally provided with a standard curve acquisition module 2, a detection module 3, a printing module 4 and a main control module 5, and the shell 1 is also provided with a sample inlet 10, a display module 11 and a scanning module 12.
And the standard curve acquisition module 2, the detection module 3, the printing module 4, the display module 11 and the scanning module 12 are respectively in information connection with the main control module 5. The main control module 5 comprises a microcontroller unit, and the main control module 5 is in information communication with the standard curve acquisition module 2, the detection module 3, the printing module 4, the display module 11 and the scanning module 12 mainly through the microcontroller unit. The microcontroller unit controls the operations of the standard curve acquisition module 2, the detection module 3, the printing module 4, the display module 11 and the scanning module 12 respectively.
The standard curve acquisition module 2 comprises an IC card reader, the data of the standard curve are written into the IC cards in advance, each IC card corresponds to a corresponding reagent and batch number, the project name and batch number information is displayed after the card is swiped, the sample result test is automatically calculated through a microcontroller unit on the main control module 5, and finally the final test result value is given.
The detection module 3 comprises a reaction cup holder, a photometry system 6 and a driving system 7, wherein the driving system 7 is connected with the reaction cup holder, and the photometry system 6 and the driving system 7 are respectively in information connection with a microcontroller unit in the main control module 5. The microcontroller unit controls the operation of the light metering system 6 and the drive system 7.
The reaction cup holder comprises a reaction cup groove 9, a reaction cup 8 is placed in the reaction cup groove 9, and a sample inlet 10 is arranged at the inlet of the reaction cup 8 placed in the reaction cup groove 9. The bottom of each reaction cup 8 is a narrow plane. The rear side of each reaction cup 8 is an inclined plane, the rear side of the reaction cup groove 9 is provided with a slope matched with the rear side of the reaction cup 8, and the design of the slope can prevent the reaction cup 8 from being misplaced.
The reaction cup 8 is preferably a colorless transparent six-way cup, and the reaction cup groove 9 is made of dark opaque materials. The driving system 7 comprises a conveying motor, drives the reaction cup holder to slide in the shell 1 along the axial direction of the reaction cup holder, and further can carry out sample feeding and conveying on the reaction cup 8.
The side of the reaction cup groove 9 is provided with small holes corresponding to the position of each reaction cup 8, namely, the side of the reaction cup groove 9 is uniformly provided with 6 small holes, and the height of the small holes is not higher than the height of the liquid level in the reaction cup 8. The diameter of the small hole on the side surface of the reaction cup groove 9 is preferably 1mm, and the small hole is preferably arranged at the middle of the side surface of the reaction cup groove 9 corresponding to the middle lower part of the reaction cup 8.
The bottom of the reaction cup groove 9 is provided with a magnetic part, preferably a magnet, at a position corresponding to each reaction cup 8. The reagent in the cuvette 8 contains a reagent and a sample to be measured. The reagent comprises a reagent A and a reagent B, wherein the reagent A contains magnetic particles, and antigens or antibodies can be coated on the magnetic particles; the reagent B contains non-magnetic color latex microspheres, and the non-magnetic color latex microspheres can also be coated with antigen or antibody. When the test solution reacts, the reagent A is added first, then the sample to be tested is added, and substances in the sample to be tested can be combined with antigens or antibodies on the magnetic particles; then adding a reagent B, wherein the antibody or antigen on the non-magnetic color latex microsphere in the reagent B is combined with another site of the substance to be detected, and finally forming a magnetic particle-antigen (antibody) -substance to be detected-latex microsphere compound, precipitating the compound along with the magnetic particles under the action of an externally applied magnetic field of a magnet at the bottom of a reaction cup groove 9, and leaving the latex microsphere which is not combined in a supernatant, wherein the color in the supernatant and the concentration of the substance to be detected are in a certain proportion.
Preferably, the color of the test solution varies in a range between red and blue. The immunoassay analyzer of the utility model mainly measures the test solution in the color change range, and calculates the concentration data of the substance to be measured through a microcontroller unit on the main control module 5 by collecting the information of the color of the supernatant.
The immunoassay analyzer of the present utility model mainly collects color information of a test solution through a photometry system 6. The photometry system 6 comprises a light source and a signal collector 61, wherein the light source is arranged outside the side surface of the reaction cup groove 9; the signal collector 61 is arranged above the reaction cup 8, and the signal collector 61 comprises a receiving sensor.
The light source is an LED light source and is used for irradiating the test solution in the reaction cup 8. Light from the light source directly enters the reaction cup 8 from the small hole on the side surface of the reaction cup groove 9, and the signal collector 61 above the reaction cup 8 absorbs the light refracted by the reaction cup 8. The signal collector 61 receives the light signal through the receiving sensor and transmits the collected light signal to the main control module 5; the microcontroller unit reads the transmitted light signal, converts the light signal into a digital signal, calculates the digital signal according to a formula, and outputs a calculation result.
The data result can be transmitted to the printing module 4 and the display module 11 through the main control module 5.
The display module 11 is a touch screen, preferably 7 inches. The display module 11 and the main control module 5 are in bidirectional transmission, and the display module 11 comprises a program setting inlet which can input project test operation, system management operation, quality control operation, project setting and the like to the instrument; the display module 11 is also provided with a result inquiry outlet, a record outlet and the like, so that the tested data result and the stored past data result can be inquired.
The printing module 4 comprises a thermal printer, and a paper outlet of the thermal printer is arranged on the shell 1. The thermal printer can print data results transmitted from the micro-controller unit, and paper data results are transmitted from the paper outlet on the housing 1.
The scanning module 12 of this embodiment is disposed on a side of the housing 1, and the scanning module 12 scans the graphic code attached to the package of the sample to be tested, where the graphic code in this embodiment is a one-dimensional code, and the one-dimensional code contains information such as name, age, sex, and number of the person of the sample to be tested.
Workflow of the immunoassay analyzer of the present embodiment:
(1) Adding a reagent A, a reagent B and a sample to be tested into a reaction cup 8 for reaction;
(2) After the reaction of the test solution in the reaction cup 8 is completed, the test solutions with different colors are formed in the reaction cup 8 due to different contents of the to-be-tested objects in the to-be-tested sample;
(3) Putting the reaction cup into a reaction cup groove 9 through a sample inlet 10 on the shell 1, and covering an outer cover;
(4) Starting a light source, wherein the light source irradiates into the reaction cup 8 from a small hole on the side surface of the reaction cup groove 9, and a signal collector 61 above the reaction cup 8 collects light signals refracted from the reaction cup 8; the driving system 7 drives the reaction cup holder to slide, and the reaction cup 8 slides along with the reaction cup holder, so that the light source irradiates each small cup in the reaction cup 8 in sequence;
(5) The main control module 5 receives the light signal transmitted by the signal collector 61, and the microcontroller unit in the main control module 5 converts the light signal into a digital signal so as to calculate the content of the object to be detected;
(6) The main control module 5 transmits the calculation result to the display module 11 and the printing module 4, the calculation result can be displayed on the display module 11, and the printing module 4 can print out the calculation result.
Claims (10)
1. The immunoassay analyzer comprises a shell (1), wherein a standard curve acquisition module (2), a detection module (3), a printing module (4) and a main control module (5) are arranged in the shell (1), and the immunoassay analyzer is characterized in that the standard curve acquisition module (2), the detection module (3) and the printing module (4) are respectively in information connection with the main control module (5); the detection module (3) comprises a reaction cup holder, a light measuring system (6) and a driving system (7), wherein the driving system (7) is connected with the reaction cup holder, and the light measuring system (6) and the driving system (7) are respectively in information connection with the main control module (5).
2. An immunoassay analyzer according to claim 1, wherein the reaction cup holder is a reaction cup groove (9), and the reaction cup (8) is placed in the reaction cup groove (9); the driving system (7) drives the reaction cup holder to slide horizontally in the shell (1).
3. An immunoassay analyzer according to claim 2, wherein the reaction cup (8) is a colorless transparent multi-cup, and the reaction cup groove (9) is made of dark opaque material; the driving system (7) drives the reaction cup holder to slide along the axial direction.
4. An immunoassay instrument according to claim 3, wherein the photometric system (6) comprises a light source and a signal collector (61), the light from the light source is directly incident into the cuvette (8), and the signal collector (61) absorbs the light refracted or reflected from the cuvette (8).
5. The immunoassay analyzer according to claim 4, wherein the side surface of the reaction cup groove (9) is provided with small holes at positions corresponding to each reaction cup (8), and the height of the small holes is not higher than the height of the liquid level in the reaction cup (8); the light source directly irradiates into the reaction cup (8) from the small hole, and the signal collector (61) above the reaction cup (8) absorbs the light signal refracted out from the reaction cup (8).
6. An immunoassay analyzer according to claim 5, wherein the bottom of the cuvette slot (9) is provided with magnetic elements at positions corresponding to each cuvette (8).
7. The immunoassay analyzer according to claim 5 or 6, wherein the housing (1) is further provided with a sample inlet (10), a display module (11) and a scanning module (12), the sample inlet (10) is used for placing the reaction cup (8) into the reaction cup groove (9), the scanning module (12) scans graphic codes on the sample package to be measured, the display module (11) and the scanning module (12) are respectively in information connection with the main control module (5), and the main control module (5) comprises a microcontroller unit.
8. The immunoassay analyzer according to claim 7, wherein the standard curve acquisition module (2) comprises an IC card reader, the light source is an LED light source, the signal acquisition device (61) comprises a receiving sensor, and the printing module (4) comprises a thermal printer.
9. The immunoassay analyzer according to claim 8, wherein the diameter of the small hole on the side of the cuvette slot (9) is 1mm, and the small hole is provided in the middle of the side of the cuvette slot (9) corresponding to the middle lower portion of the cuvette (8).
10. The immunoassay analyzer according to claim 9, wherein the reaction cups (8) are six-way cups, the bottom of each reaction cup (8) is a narrow plane, the rear side of each reaction cup (8) is an inclined plane, and the rear side of the reaction cup groove (9) is provided with an inclined plane which is matched with the rear side of the reaction cup (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223288697.6U CN219455951U (en) | 2022-12-08 | 2022-12-08 | Immunoassay instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223288697.6U CN219455951U (en) | 2022-12-08 | 2022-12-08 | Immunoassay instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219455951U true CN219455951U (en) | 2023-08-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223288697.6U Active CN219455951U (en) | 2022-12-08 | 2022-12-08 | Immunoassay instrument |
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|---|---|
| CN (1) | CN219455951U (en) |
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2022
- 2022-12-08 CN CN202223288697.6U patent/CN219455951U/en active Active
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