CN219449723U - Bioreactor bag and bioreactor - Google Patents

Abstract

The utility model relates to a biological reaction bag and a biological reactor, which comprises a bag body and an air inlet assembly, wherein the air inlet assembly comprises an air inlet pipe and an aeration piece, an opening is formed in the bag body, the air inlet pipe penetrates through the opening and is in sealing connection with the bag body, the aeration piece is accommodated in the bag body, and the air inlet pipe is communicated with the aeration piece. The air inlet component is preassembled on the bag body, so that the secondary installation step is omitted, and the installation and the use are simplified; the air inlet pipe is sealed with the bag body at the opening, so that the tightness of the biological reaction bag is ensured, the leakage of gas or culture solution is avoided, external impurities are prevented from entering the bag body, the cell culture solution is polluted, and the tightness of the biological reaction bag is improved; the aeration piece is used for conveying gas into the culture solution, so that cells can be effectively prevented from flowing back into the air inlet pipe along with the culture solution; all parts in the biological reaction bag are disposable products, and sterilization or cleaning steps are not needed when the biological reaction bag is used, so that the risk of cross contamination is reduced to the maximum extent, and the biological reaction bag has wider application prospect.

Description

Bioreactor bag and bioreactor

Technical Field

The utility model relates to the technical field of biological reaction equipment, in particular to a biological reaction bag and a biological reactor.

Background

With the development of the technology of biological reaction equipment, the stirring type biological reactor is widely applied to biological reaction engineering. A stirred bioreactor with a disposable bioreactor bag is favored by the bioengineering industry because of the advantages of no need of sterilization or cleaning and reduced risk of cross contamination.

In the prior art, a bag body of the biological reaction bag and an air inlet assembly are arranged in a split mode, an air inlet is arranged above the biological reaction bag, an air inlet pipe is inserted from the air inlet and stretches into the position below the liquid level, and oxygen enters culture solution through the air inlet pipe to supply oxygen for cell reaction. However, before the bioreactor bag is used, a professional technician is required to assemble the air inlet assembly with the bag body, and the bioreactor bag is inconvenient to use.

Disclosure of Invention

Based on this, it is necessary to provide a bioreactor bag and a bioreactor.

The utility model provides a biological reaction bag, includes the bag body and the subassembly that admits air, the subassembly that admits air includes intake pipe and aeration spare, the opening has been seted up on the bag body, the intake pipe wears to establish the opening part of the bag body and with bag body sealing connection, aeration spare holding in the bag body, just the intake pipe with aeration spare is linked together.

The air inlet component is preassembled on the bag body, so that the secondary installation step is omitted, and the installation and the use are simplified; the air inlet pipe is sealed with the bag body at the opening of the bag body, so that the tightness of the biological reaction bag is ensured, the leakage of gas or culture solution is avoided, external impurities are prevented from entering the bag body, the cell culture solution is polluted, and the tightness of the biological reaction bag is improved; the aeration piece is used for conveying gas into the culture solution, so that cells can be effectively prevented from flowing back into the air inlet pipe along with the culture solution; all parts in the biological reaction bag are disposable products, and sterilization or cleaning steps are not needed when the biological reaction bag is used, so that the risk of cross contamination is reduced to the maximum extent, and the biological reaction bag has wider application prospect.

In one embodiment, the position of the opening corresponds to the position of an electrode window on the culture vessel.

So set up, the opening on the bag body concentrates on the position of electrode window, has made things convenient for the unified management of all kinds of pipelines, has utilized the electrode window on the culture tank simultaneously, does not have to be in order to adapt the open position of biological reaction bag and reaperture on the culture tank, has reduced manufacturing cost and use cost, can also reduce the length of pipeline in the bag simultaneously.

In one embodiment, the bioreactor bag further comprises a stirring assembly, the stirring assembly comprises a main shaft and a plurality of paddles arranged on the main shaft, and the air inlet pipe in the bag body is bent towards the bottom of the bag body so as to avoid the paddles.

The arrangement ensures that the gas is uniformly dispersed in the culture solution by stirring, increases the contact area of the gas and the liquid, and ensures that the gas introduced into the bag is more fully dissolved in the culture solution; the air inlet pipe is bent towards the bottom of the bag body in the bag body, so that the paddles are avoided, and damage to cells caused by shearing force formed by too close distance between the air inlet pipe and the paddles in the culture solution stirring process is prevented.

In one embodiment, the number of air inlet pipes is multiple, a plurality of air outlet holes with gears are formed in the aeration piece, and each air outlet hole with gears corresponds to at least one air inlet pipe.

So set up, outside air feed equipment controls the intake pipe that each fender venthole corresponds respectively, can accurate control cultivate the oxygen content in the jar, is applicable to the scene of different cell cultures.

In one embodiment, the number of the air inlet pipes is two, and the air outlet holes of the large bubble blocking position and the small bubble blocking position are formed in the aeration piece.

So set up, the aeration piece has two at least and keeps off the regulation and control, is big bubble respectively and keeps off and keep off with little bubble, and aeration amount accessible according to different keeps off the position adjustment of aeration piece, has increased the service scenario.

In one embodiment, a plurality of the air inlet pipes are arranged on the same side of the aeration member.

The plurality of air inlet pipes can extend out of the culture tank through the electrode window, so that unified management is facilitated; and the length of the air inlet pipe in the bag body is shorter, so that the air inlet pipe and the blade are prevented from being interfered or wound in the stirring process.

In one embodiment, a plurality of the air inlet pipes are spaced around the aeration member.

The number of the air inlet pipes can be increased, the gear accuracy can be increased, and the cell reaction can be finely controlled.

In one embodiment, the aeration piece comprises an aeration disc and a plurality of connectors protruding from the peripheral wall of the aeration disc, and the connectors are used for being connected with the air inlet pipe;

the aeration disc is provided with the air outlet holes, and the air outlet holes are arranged in an annular array.

So set up, the aeration piece is given vent to anger evenly, under the stirring effect, and the gas of input biological reaction bag can evenly disperse in the culture solution, avoids gas dispersion inhomogeneous influence cell culture process.

In one embodiment, the bioreactor bag further comprises a stirring assembly and a first connecting piece, wherein the stirring assembly comprises a main shaft and a plurality of paddles arranged on the main shaft, the first connecting piece is arranged at the bottom of the bag body, and the bottom of the main shaft and the aeration piece are both connected with the first connecting piece.

So set up, adopt first connecting piece to inject the bottom of main shaft, avoided the rocking of main shaft when stirring, stirring subassembly and aeration piece all are connected in first connecting piece, have reduced connecting piece quantity, have reduced installation cost.

In one embodiment, the first connecting piece comprises a welding disc and a clamping structure which are connected with each other, the first connecting piece is fixed at the bottom of the bag body through welding of the welding disc, the main shaft stretches into the clamping structure and is fixedly connected with the clamping structure, and the aeration disc is clamped on the peripheral wall of the clamping structure.

The main shaft and the aeration disc are fixed through the clamping structure, so that the integration level and the integrity of the biological reaction bag are improved; the stirring assembly is fixed at the position of the stirring assembly through the main shaft, and the aeration disc is directly fixed on the clamping structure, so that shaking caused by water flow can be effectively avoided in use.

In one embodiment, the air inlet assembly further comprises a second connecting piece, the air inlet pipe comprises an inner bag pipe and an outer bag pipe, two ends of the inner bag pipe are respectively connected with the aeration piece and the second connecting piece, and two ends of the outer bag pipe are respectively connected with the second connecting piece and the external air supply device.

So set up, the bag inner tube passes through the second connecting piece with the bag outer tube and is connected, and bag inner tube and bag outer tube each other do not influence when the installation, can adjust the model category of bag inner tube or bag outer tube as required, and it is convenient to expand, and the suitability is good.

In one embodiment, a welding ring is convexly arranged on the outer peripheral wall of the air inlet pipe, and the welding ring is fixed at the opening in a welding mode.

So set up, the welding area of intake pipe and opening part has been increased in the setting of weld ring, firm in connection, and intake pipe formula pipeline as an organic whole, and the continuity is good.

In one embodiment, the bioreactor bag further comprises a filter, wherein the filter is positioned outside the bag body and communicated with the air inlet pipe, and the filter is used for filtering the air provided by the external air supply equipment.

The arrangement ensures the purity of the input gas and effectively reduces the influence of impurities in the gas on the cell culture process.

The utility model also relates to a bioreactor which comprises a culture tank and the biological reaction bag, wherein the biological reaction bag is accommodated in the culture tank.

So set up, paste in the culture tank in the biological reaction bag, at cell culture in-process, the bag body is fixed by the culture tank, and the bag body is difficult to warp when stirring.

In one embodiment of the utility model, an electrode window is formed in the outer peripheral wall of the lower side of the culture tank.

So set up, the pipeline of biological reaction bag can concentrate and set up in electrode window department, and monitoring or check out test set can also set up here, is favorable to centralized management.

Drawings

FIG. 1 is a schematic view of a bioreactor bag according to an embodiment of the present utility model;

FIG. 2 is a schematic view showing the structure of a bioreactor according to an embodiment of the present utility model;

FIG. 3 is a schematic view showing the structure of an aeration device according to an embodiment of the present utility model;

FIG. 4 is a schematic view showing the structure of a bioreactor bag according to another embodiment of the present utility model;

FIG. 5 is a cross-sectional view of a bioreactor bag according to another embodiment of the present utility model;

FIG. 6 is an enlarged view of a portion of the X portion of FIG. 5;

FIG. 7 is a schematic view of a second connector;

fig. 8 is a side view of the second connector shown in fig. 7.

Reference numerals:

100. a biological reaction bag; 10. a bag body; 11. an opening; 20. a stirring assembly; 21. a main shaft; 22. a paddle; 30. an air intake assembly; 31. an air inlet pipe; 311. a bag inner tube; 312. a bag outer tube; 32. an aeration member; 321. an aeration disc; 3211. an air outlet hole; 322. a joint; 33. a second connector; 331. a mounting head; 332. welding plates; 40. a first connector; 41. a welding plate; 42. a clamping structure; 50. a filter;

200. a culture tank; 201. an electrode window; 300. a bioreactor.

Detailed Description

In order that the above objects, features and advantages of the utility model will be readily understood, a more particular description of the utility model will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present utility model. The present utility model may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the utility model, whereby the utility model is not limited to the specific embodiments disclosed below.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present utility model, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.

In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present utility model, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.

Referring to fig. 1 and 2, fig. 1 is a schematic diagram illustrating a bioreactor bag 100 according to an embodiment of the utility model, and fig. 2 is a schematic diagram illustrating a bioreactor 300 according to an embodiment of the utility model. A common field of application of the bioreactor bag 100 is animal and plant cell culture, and can also be applied to microbial fermentation.

The biological reaction bag 100 comprises a bag body 10, a stirring assembly 20 and an air inlet assembly 30, wherein the stirring assembly 20 is arranged in the bag body 10, an opening 11 is formed in the bag body 10, and the air inlet assembly 30 supplies air into the bag body 10 through the opening 11.

In the prior art, a bag body of the biological reaction bag and an air inlet assembly are arranged in a split mode, an air inlet is arranged above the biological reaction bag, an air inlet pipe is inserted from the air inlet and stretches into the position below the liquid level, and oxygen enters culture solution through the air inlet pipe to supply oxygen for cell reaction. However, before the bioreactor bag is used, a professional technician is required to assemble the air inlet assembly with the bag body, and the bioreactor bag is inconvenient to use.

Based on this, in the present utility model, the air intake assembly 30 includes an air intake pipe 31 and an aeration member 32, the bag body 10 is provided with an opening 11, the air intake pipe 31 passes through the opening 11 and is connected with the bag body 10 in a sealing manner, the aeration member 32 is accommodated in the bag body 10, and the air intake pipe 31 is communicated with the aeration member 32.

The air inlet assembly 30 is preassembled on the bag body 10, so that the secondary installation step is omitted, the installation and the use are simplified, the air inlet pipe 31 is sealed with the bag body 10 at the opening of the bag body 10, the tightness of the biological reaction bag 100 is ensured, the leakage of air or culture solution is avoided, the external impurities are prevented from entering the bag body 10, the tightness of the biological reaction bag 100 is ensured, the liquid is conveyed into the culture solution through the aeration piece 32, and the backflow of cells along with the culture solution into the air pipe can be effectively prevented; all parts in the biological reaction bag 100 are disposable products, and sterilization or cleaning steps are not needed when the biological reaction bag is used, so that the risk of cross contamination is reduced to the maximum extent, and the biological reaction bag has wider application prospect.

In addition, in the traditional biological reaction bag, because the air inlet is formed in the top of the bag body, the air inlet pipeline in the bag body is longer, and the pipeline is required to be fixed on the inner side of the disposable biological reaction bag through a plurality of supporting pieces, so that the production cost is increased.

Alternatively, in one embodiment of the present utility model, the position of the opening corresponds to the position of the electrode window 201 on the culture tank 200.

So set up, the opening on the bag body 10 concentrates on the position of electrode window 201, has made things convenient for the unified management of all kinds of pipelines, has utilized electrode window 201 on the culture tank 200 simultaneously, avoids reopening on the culture tank 200 in order to adapt the open position of biological reaction bag 100, has reduced manufacturing cost and use cost.

Optionally, as shown in fig. 1, in an embodiment of the present utility model, the bioreactor bag further includes a stirring assembly 20, the stirring assembly 20 includes a main shaft 21 and a plurality of paddles 22 disposed on the main shaft 21, and an air inlet pipe 31 disposed in the bag 10 is bent toward the bottom of the bag 10 to avoid the paddles 22.

The biological reaction bag is arranged in such a way that the gas is uniformly dispersed in the culture solution through the stirring assembly 20, the contact area of the gas and the liquid is increased, and the gas introduced into the bag is more fully dissolved in the culture solution; the air inlet pipe 31 is bent towards the bottom of the bag body 10 in the bag body 10, so that the paddle 22 is avoided, and damage to cells caused by shearing force formed by too close distance between the air inlet pipe 31 and the paddle 22 in the process of stirring the culture solution is prevented.

It will be appreciated that in other embodiments, the air inlet tube 31 is disposed and secured against the side walls and bottom of the bioreactor bag 100.

So set up, whether in the state of admitting air or in the stirring in-process, intake pipe 31 can not rock, and keep away from paddle 22, reduced intake pipe 31 to the interference of cell culture process.

In the prior art, the aeration disc has different types or specifications, different aeration discs can be selected according to different cells, and the aeration disc and an air inlet pipeline are required to be assembled secondarily, so that the aeration disc is inconvenient and the risk of cross contamination exists during bag opening and installation.

Referring to fig. 3, fig. 3 is a schematic view illustrating a structure of an aeration device 32 according to an embodiment of the present utility model.

Optionally, in one embodiment of the present utility model, the air inlet pipe 31 is multiple, the aeration member 32 is provided with a plurality of air outlet holes 3211 with a plurality of steps, and each air outlet hole 3211 with a plurality of steps corresponds to at least one air inlet pipe 31.

So set up, the outside air supply equipment controls the intake pipe 31 that each keeps off venthole 3211 corresponds respectively, can accurate control cultivate the oxygen content in jar 200, is applicable to the scene of different cell cultures.

It will be appreciated that in other embodiments, the air inlet pipe 31 is a single pipe, the aeration disc 321 is provided with air outlet holes 3211 with a fixed size, and the external air supply device (not shown) controls the air inlet amount by adjusting the air supply rate.

So set up, aeration dish 321 simple structure is convenient for produce, and the air input is decided by the air feed rate of outside air feed equipment, and the air input is estimated easily.

Optionally, in one embodiment of the present utility model, the number of air inlet pipes 31 is two, and the air outlet holes 3211 of the large bubble blocking position and the small bubble blocking position are formed on the aeration member 32.

So set up, aeration piece 32 can be divided into two at least and keep off the regulation and control, is big bubble fender, little bubble fender respectively, and aeration amount accessible according to different fender position adjustment of aeration piece 32 has increased the service scenario.

Of course, the large bubble block and the small bubble block can be opened simultaneously to increase the air inflow.

By the arrangement, the range of the air output of the aeration disc 321 is expanded, and the use scene is further increased.

Alternatively, referring to fig. 1 to 3, in one embodiment of the present utility model, a plurality of air inlet pipes 31 are provided at the same side of the aeration member 32.

So arranged, the plurality of air inlet pipes 31 can extend out of the culture tank 200 through the electrode window 201, so that unified management is facilitated; and, the length of the air inlet pipe 31 in the bag body 10 is shorter, so that the air inlet pipe 31 and the blade 22 are prevented from being interfered or wound in the stirring process.

Referring to fig. 4, fig. 4 is a schematic structural diagram of a bioreactor bag 100 according to another embodiment of the utility model.

Alternatively, in one embodiment of the present utility model, a plurality of air inlet pipes 31 are spaced around the aeration member 32.

By the arrangement, the number of the air inlet pipes 31 can be increased, the gear accuracy can be increased, and the cell reaction can be finely controlled.

Optionally, in one embodiment of the present utility model, the aeration member 32 includes an aeration disc 321 and a plurality of connectors 322 protruding from the peripheral wall of the aeration disc 321, where the connectors 322 are used to connect with the air inlet pipe 31; the aeration plate 321 is provided with air outlet holes 3211, and the air outlet holes 3211 are arranged in a ring array.

So set up, aeration piece 32 is given vent to anger evenly, under the stirring effect, and the gas of input biological reaction bag 100 can evenly disperse in the culture solution, avoids the local gaseous accumulation in the culture solution to produce the influence to the cell culture process.

Alternatively, the air outlet holes 3211 of the same gear are located on the same circumference.

It will be appreciated that in other embodiments, the larger and smaller gas outlet holes 3211, 3211 are spaced apart in the radial direction of the aeration disc 321.

So set up, because the culture solution velocity of flow of stirring in-process bag body 10 everywhere is different, through the size of gas vent, the position of gas vent and the velocity of flow of culture solution, can control dissolved oxygen efficiency more accurately.

Referring to fig. 5 and 6, fig. 6 is a sectional view of a bioreactor bag 100 according to another embodiment of the present utility model shown in fig. 5, and fig. 6 is a partial enlarged view of the portion X shown in fig. 5.

Optionally, in one embodiment of the present utility model, the bioreactor bag 100 includes a stirring assembly 20 and a first connecting member 40, the stirring assembly 20 includes a main shaft 21 and a plurality of paddles 22 disposed on the main shaft 21, the first connecting member 40 is disposed at the bottom of the bag body 10, and the bottom of the main shaft 21 and the aeration member 32 are connected to the first connecting member 40.

So set up, adopt first connecting piece 40 to inject the bottom of main shaft 21, avoided the rocking of main shaft 21 when stirring, stirring subassembly 20 and aeration piece 32 all are connected in first connecting piece 40, have reduced the quantity of connecting piece, have reduced the installation cost.

It will be appreciated that in other embodiments, the stirring assembly 20 may be coupled to the aeration member 32, and the aeration member 32 is fixedly coupled to the bottom center within the pouch 10.

So configured, the aeration member 32 is directly fixed inside the bioreactor bag 100, and the integrity of the bioreactor bag 100 is increased.

Alternatively, in one embodiment of the present utility model, the first connecting member 40 includes a welding disc 41 and a clamping structure 42 that are connected to each other, the first connecting member 40 is welded and fixed to the bottom of the bag body 10 by the welding disc 41, the main shaft 21 extends into the clamping structure 42 and is fixedly connected to the clamping structure 42, and the aeration disc 321 is clamped to the outer peripheral wall of the clamping structure 42.

So arranged, the main shaft 21 and the aeration disc 321 are fixed through the clamping structure 42, so that the integration level and the integrity of the biological reaction bag 100 are improved; the stirring assembly 20 is fixed at the position through the main shaft 21, and the aeration disc 321 is directly fixed on the clamping structure 42, so that shaking caused by water flow can be effectively avoided in use.

Referring to fig. 1, 7 and 8, fig. 7 is a schematic structural view of the second connecting member 33, and fig. 8 is a side view of the second connecting member 33 shown in fig. 7.

Optionally, in an embodiment of the present utility model, the air intake assembly 30 further includes a second connecting member 33, the second connecting member 33 includes a mounting head 331 and a welding plate 332, the air intake tube 31 includes an inner tube 311 and an outer tube 312, two ends of the inner tube 311 are respectively connected to the aeration member 32 and the mounting head 331, two ends of the outer tube 312 are respectively connected to the mounting head 331 and an external air supply device, and the second connecting member 33 is fixed to the side wall opening 11 of the bag body 10 by the welding plate 332.

So set up, bag inner tube 311 passes through second connecting piece 33 with bag outer tube 312 to be connected, and bag inner tube 311 does not influence each other with bag outer tube 312 when the installation, can adjust the model category of bag inner tube 311 or bag outer tube 312 as required, and it is convenient to extend, and the suitability is good.

It will be appreciated that in other embodiments, the air intake assembly 30 further includes a second connector 33, where a bending portion (not shown) is disposed at an end of the second connector 33 connected to the inner bag tube 311, and the second connector 33 is mounted and fixed along the bending portion toward the bottom of the bag body 10.

So set up, bag inner tube 311 can press close to bag body 10 lateral wall after the installation is accomplished, avoids the bag inner tube to buckle, influences air inlet efficiency.

Alternatively, in one embodiment of the present utility model, a welding ring is protruded on the outer circumferential wall of the air inlet tube 31, and the welding ring is welded and fixed at the opening 11 of the bag body 10.

So set up, the welding area of intake pipe 31 and the department of bag body 10 opening 11 has been increased in the setting of weld ring, firm in connection, and intake pipe 31 pipeline as an organic whole, and the continuity is good.

It will be appreciated that in other embodiments, the outer peripheral wall of the air inlet pipe 31 is provided with a weld ring (not shown) protruding therefrom, the annulus of the weld ring intersecting the cross-section of the air inlet pipe at an incline.

By means of the arrangement, the air inlet pipe 31 is obliquely inserted into the bag body 10, the bending degree of the air inlet pipe 31 is reduced, and the air inlet efficiency is prevented from being influenced due to the fact that the air inlet pipe 31 is partially closed due to bending.

Optionally, in one embodiment of the present utility model, the bioreactor bag 100 further comprises a filter 50, wherein the filter 50 is located outside the bag body 10 and is connected to the air inlet pipe 31, and the filter 50 is used for filtering the air provided by the external air supply device.

The arrangement ensures the purity of the input gas and effectively reduces the influence of impurities in the gas on the cell culture process. In addition, the filter is integrated on the pouch body 10, and the bioreactor pouch 100 has a filtering function, thereby simplifying installation.

It will be appreciated that in other embodiments, the second connector 33 is further provided with a filtering portion (not shown), and the gas input from the gas inlet pipe 31 enters the bag 10 after being filtered by the filtering portion.

The present utility model also provides a bioreactor 300, comprising a culture tank 200 and a bioreactor bag 100, wherein the bioreactor bag 100 comprises the bioreactor bag 100, and the bioreactor bag 100 is accommodated in the culture tank 200.

In this way, the bioreactor bag 100 is attached to the culture tank 200, and the shape of the bag 10 is fixed by the culture tank 200 during cell culture, so that the bag 10 is not easily deformed during stirring.

Alternatively, in one embodiment of the present utility model, an electrode window 201 is formed in the outer peripheral wall of the lower side of the culture tank 200.

So arranged, the piping of the bioreactor bag 100 can be arranged at the electrode window 201 in a centralized manner, and the monitoring or detecting equipment can be arranged at the electrode window, which is beneficial to centralized management.

It will be appreciated that in other embodiments, a transparent window (not shown) is also provided in the side wall of the culture tank 200.

The device can be used for monitoring and observing in real time in the cell culture process, so that experiments or production personnel can conveniently and pertinently adjust the culture scheme.

The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the utility model. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the utility model, which are all within the scope of the utility model. Accordingly, the scope of protection of the present utility model is to be determined by the appended claims.

Claims (14)

1. The utility model provides a biological reaction bag, its characterized in that includes the bag body and admits air the subassembly, the subassembly that admits air includes intake pipe and aeration spare, the opening has been seted up on the bag body, the intake pipe wears to establish the opening part of the bag body and with bag body sealing connection, aeration spare holding in the bag is internal, just the intake pipe with aeration spare is linked together.

2. The bioreactor bag of claim 1, wherein the opening is positioned to correspond to the position of an electrode window on the culture tank.

3. The bioreactor bag of claim 2, further comprising a stirring assembly, wherein the stirring assembly comprises a main shaft and a plurality of paddles disposed on the main shaft, and the air inlet pipe in the bag body is bent towards the bottom of the bag body so as to avoid the paddles.

4. The bioreactor bag of claim 1, wherein the number of air inlet pipes is multiple, a plurality of air outlet holes with gears are formed in the aeration piece, and each air outlet hole with gears corresponds to at least one air inlet pipe.

5. The bioreactor bag of claim 4, wherein the number of air inlet pipes is two, and the aeration piece is provided with the air outlet holes of a large bubble blocking position and a small bubble blocking position.

6. The bioreactor bag of claim 4, wherein a plurality of said air inlet pipes are provided on the same side of said aeration member; alternatively, a plurality of the air inlet pipes are spaced around the aeration member.

7. The bioreactor bag of claim 4, wherein the aeration member comprises an aeration disc and a plurality of connectors protruding from the peripheral wall of the aeration disc, and the connectors are used for being connected with the air inlet pipe;

the aeration disc is provided with the air outlet holes, and the air outlet holes are arranged in an annular array.

8. The bioreactor bag of claim 1, further comprising a stirring assembly and a first connector, wherein the stirring assembly comprises a main shaft and a plurality of paddles disposed on the main shaft, the first connector is disposed at the bottom of the bag body, and the bottom of the main shaft and the aerator are both connected to the first connector.

9. The bioreactor bag of claim 8, wherein the first connecting piece comprises a welding disc and a clamping structure which are connected with each other, the first connecting piece is welded and fixed at the bottom of the bag body through the welding disc, the main shaft stretches into the clamping structure and is fixedly connected with the clamping structure, and the aeration piece is clamped on the peripheral wall of the clamping structure.

10. The bioreactor bag according to claim 1, wherein the air inlet assembly further comprises a second connecting piece, the air inlet pipe comprises an inner bag pipe and an outer bag pipe, two ends of the inner bag pipe are respectively connected with the aeration piece and the second connecting piece, and two ends of the outer bag pipe are respectively connected with the second connecting piece and an external air supply device.

11. The bioreactor bag of claim 1, wherein a welding ring is arranged on the outer peripheral wall of the air inlet pipe in a protruding mode, and the welding ring is fixed at the opening in a welding mode.

12. The bioreactor bag of claim 1, further comprising a filter located outside the bag body and in communication with the air inlet tube, the filter for filtering air provided by an external air supply.

13. A bioreactor comprising a culture tank and a bioreactor bag as claimed in any one of claims 1 to 12, the bioreactor bag being housed within the culture tank.

14. The bioreactor of claim 13, wherein an electrode window is formed in the peripheral wall of the lower side of the culture tank.