CN219409738U

CN219409738U - Semi-continuous small bioreactor

Info

Publication number: CN219409738U
Application number: CN202320069933.0U
Authority: CN
Inventors: 陈聪; 孙宇杰; 姚洁; 刘刚金; 宋扬; 刘京
Original assignee: Bipu Instrument Haining Co ltd
Current assignee: Bipu Instrument Haining Co ltd
Priority date: 2023-01-10
Filing date: 2023-01-10
Publication date: 2023-07-25
Anticipated expiration: 2033-01-10

Abstract

The utility model discloses a semi-continuous small bioreactor, which comprises a reactor body, wherein four hole sites are arranged on the upper part of the reactor body, namely a hole site I, a hole site II, a hole site III and a hole site IV, and an upper cover is arranged on each of the four hole sites; the PH meter is connected to the hole site I, the glass funnel and the glass elbow are respectively connected to the hole site II and the hole site IV, and the double-hole plug is connected to the hole site III. The utility model discloses can be applicable to laboratory and small-size pilot scale for carry out conventional experiment in the laboratory, accomplish simultaneously and add the material, sample, detect PH and carry out the function of stirring to the material and need.

Description

Semi-continuous small bioreactor

Technical Field

The utility model relates to the field of bioreactors, in particular to a semi-continuous small bioreactor.

Background

At present, when a semi-continuous biological reaction experiment is carried out, fresh materials are needed to be replenished into the bioreactor on time, products are discharged from the bioreactor, meanwhile, the biological reaction process is known by analyzing the gas production condition, the PH value in the reactor and the like, and the running stability of the reactor is judged. In the conventional semi-continuous biological reaction test, the bioreactor is required to be provided with a material adding port, a sampling port, a gas detecting port and a standby port for adding detection instruments for other purposes such as a PH meter. Only large or ultra-large reactors have such functions in the market at present, the reactors with small capacity are mostly single-hole, a small amount of holes do not meet the functions required by biological experiments, the large capacity is too large, and the large capacity is suitable for engineering and does not meet the laboratory requirements, so that the small semi-continuous biological experiments on laboratory scale are more needed to be suitable for the reactors on laboratory and small pilot scale.

Disclosure of Invention

The technical problem to be solved by the utility model is to provide a semi-continuous small bioreactor which can be suitable for laboratories and small pilot scale and is used for carrying out routine experiments in the laboratories, and the functions of adding materials, sampling, detecting PH and stirring the materials can be simultaneously realized.

The technical scheme adopted for solving the technical problems is as follows: a semi-continuous small bioreactor comprises a reactor body, wherein four hole sites, namely a hole site I, a hole site II, a hole site III and a hole site IV, are arranged at the upper part of the reactor body, and are respectively provided with an upper cover; the PH meter is connected to the hole site I, the glass funnel and the glass elbow are respectively connected to the hole site II and the hole site IV, and the double-hole plug is connected to the hole site III.

Preferably, the upper covers of the hole sites I, II and IV are solid covers or headspace covers.

Preferably, the two-hole plug connected with the hole site III is characterized in that one hole is connected with a gas pipe to lead out gas for measurement, and the other hole is connected with a gas pipe upper pipe clamp for sealing and screwed and sealed by a screw cap.

As a further improvement, the threaded cover is additionally provided with a stirrer.

Preferably, the reactor body is a borosilicate glass reactor body.

As a preference, the hole I is GL25, the holes II and IV are GL32, and the hole III is GL45.

The hole sites of the utility model can be sealed independently, namely, a solid cover is covered, and a corresponding accessory can be inserted into the hole of the head space cover to achieve a certain experimental purpose.

The utility model has the beneficial effects that the design of the device is provided with four holes, so that the device can respectively meet the requirements of loading materials, taking out materials, inserting detection instruments such as a PH meter and the like, and connecting an air outlet and stirring system. The experimental requirements of suitable laboratory and small pilot scale that do not have in the market at present are satisfied, accomplish simultaneously to add the material, sample, detect PH and carry out the required hole site quantity requirement of function of stirring to the material.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present utility model.

FIG. 2 is a schematic top view of a reactor body according to an embodiment of the present utility model.

Wherein, 1, a reactor body; 2. a GL25 specification headspace cover; 3. a glass funnel; 4. a glass elbow; 5. a GL32 specification headspace cover; 6. GL45 screw cap; 7. a double hole plug; 8. GL25 specification hole site I; 9. GL32 specification hole site II; 10. GL45 specification hole site III; 11. GL32 gauge hole site IV.

The utility model is further described below with reference to the accompanying drawings.

Detailed Description

Example 1: as shown in fig. 1 and 2, a semi-continuous small bioreactor comprises a reactor body 1, wherein the reactor body 1 is a borosilicate glass reactor body. Four hole sites are arranged on the upper portion of the reactor body 1 and are respectively a GL25 specification hole site I8, a GL32 specification hole site II 9, a GL45 specification hole site III 10 and a GL32 specification hole site IV 11, upper covers are arranged on the four hole sites, a GL32 specification headspace cover 2 is arranged on the hole site I8, and a PH meter is arranged through the headspace cover 2 to detect PH values. The hole site II 9 and the hole site IV 11 are provided with a GL 32-specification top hollow cover 5, and the hole site II 9 and the hole site IV 11 provided with the top hollow cover 5 are respectively connected with a glass funnel 3 and a glass bent pipe 4, wherein the glass funnel 3 is added with materials, and the glass bent pipe 4 is used for taking materials; and the hole III 10 is connected with a double-hole plug 7, one hole of the double-hole plug 7 is connected with an air pipe, gas can be led out for measurement, the other hole is connected with an air pipe, a pipe clamp is clamped and sealed, and the pipe clamp is screwed by a thread cover 6 to finish sealing.

Example 2: the second semi-continuous small bioreactor comprises a reactor body 1, four hole sites are arranged on the upper portion of the reactor body 1, a double-hole plug 7 is connected to the hole site III 10, the double-hole plug is screwed by a threaded cover 6, and a stirrer is additionally arranged on the threaded cover 6 to stir materials in the reactor body. The other steps are the same as in example 1.

Example 3: the third semi-continuous small bioreactor comprises a reactor body 1, wherein four hole sites are arranged at the upper part of the reactor body 1, the four hole sites are respectively a hole site I, a hole site II, a hole site III and a hole site IV, the hole site I is connected with a solid cover, and the sealing treatment is carried out when the reactor is not used. The hole site II and the hole site IV are provided with a top empty cover, and the hole site II and the hole site IV provided with the top empty cover are respectively connected with a glass funnel and a glass bent pipe, wherein the glass funnel is used for adding materials, and the glass bent pipe is used for taking materials; the hole III is connected with a double-hole plug 7, one hole of the double-hole plug 7 is connected with an air pipe to lead out air for measurement, the other hole is connected with an air pipe to be filled with pipe clamps for sealing, a screw cap is screwed for sealing, and a stirrer is additionally arranged on the screw cap to stir materials in the reactor body.

Claims

1. Semi-continuous small-size bioreactor, including reactor body (1), its characterized in that: four hole sites, namely a hole site I (8), a hole site II (9), a hole site III (10) and a hole site IV (11), are arranged at the upper part of the reactor body (1), and an upper cover is arranged on each of the four hole sites; the PH meter is connected to the hole site I (8), the glass funnel (3) and the glass elbow (4) are respectively connected to the hole site II (9) and the hole site IV (11), and the double-hole plug (7) is connected to the hole site III (10).

2. A semi-continuous mini-bioreactor as in claim 1, wherein: the upper covers of the hole sites I (8), II (9) and IV (11) are solid covers or headspace covers.

3. A semi-continuous mini-bioreactor as in claim 1, wherein: and a double-hole plug (7) connected with the hole III (10), wherein one hole is connected with the air pipe to lead out air, measurement is carried out, and the other hole is connected with the air pipe to form a pipe clamp seal, and the pipe clamp seal is screwed and sealed by a thread cover (6).

4. A semi-continuous mini-bioreactor as in claim 3, wherein: the threaded cover (6) is additionally provided with a stirrer.

5. A semi-continuous mini-bioreactor as in claim 1, wherein: the reactor body (1) is a high borosilicate glass reactor body.

6. A semi-continuous mini-bioreactor as in claim 1, wherein: the specification of the hole site I (8) is GL25, the specification of the hole site II (9) and the specification of the hole site IV (11) are GL32, and the specification of the hole site III (10) is GL45.