CN219409684U

CN219409684U - Vibration device for exosome separation

Info

Publication number: CN219409684U
Application number: CN202320318187.4U
Authority: CN
Inventors: 孔祥梅; 刘锐
Original assignee: Shandong Huasi Biotechnology Co ltd
Current assignee: Shandong Huasi Biotechnology Co ltd
Priority date: 2023-02-27
Filing date: 2023-02-27
Publication date: 2023-07-25
Anticipated expiration: 2033-02-27

Abstract

The utility model discloses an oscillation device for separating an exosome, which relates to the technical field of oscillation equipment and comprises a base, wherein four corners of the upper end surface of the base are fixedly connected with limiting slide bars, oscillation plates are arranged among the four limiting slide bars, the four corners of the oscillation plates are respectively provided with a sliding sleeve in sliding connection with the limiting slide bars, the upper end surface of the oscillation plates is fixedly connected with a plurality of placing cylinders, two sides of each placing cylinder are provided with side openings, each placing cylinder is also provided with a strip-shaped hole, and a fixing assembly for fixing a test tube is arranged in each strip-shaped hole; according to the utility model, the test tube can be fixed through the fixing assembly, so that the test tube can be prevented from flying out in the vibration process, and the safety of the device is greatly improved.

Description

Vibration device for exosome separation

Technical Field

The utility model relates to the technical field of oscillation equipment, in particular to an oscillation device for separating exosomes.

Background

Exosomes refer to small vesicles (30-150 nm) containing complex RNAs and proteins, which nowadays are specifically disc-shaped vesicles with diameters of 40-100 nm. A variety of cells secrete exosomes under normal and pathological conditions. The method mainly derives from a multi-vesicle formed by the invagination of intracellular lysosome particles, is released into extracellular matrix after being fused with cell membranes through multi-vesicle outer membranes, and is the most commonly used method for extracting exosome RNA at present with fewer reagents on the market. In the process of extracting exosome RNA, the reagent and the exosome are uniformly mixed, and then the exosome is placed in a test tube for precipitation, but the exosome is difficult to be fully mixed with the reagent due to the extremely viscous character, and the reagent needs manual vibration, which is time-consuming and labor-consuming.

Based on the above-mentioned problems, CN 213032364U proposes an oscillation device for separating exosomes, which comprises a bottom plate, wherein the upper side of the bottom plate is fixedly connected with the lower ends of a group of uniformly arranged L-shaped rod vertical rods, and the upper side of the bottom plate is fixedly connected with the lower side of a mounting seat; the opposite ends of the L-shaped rod cross rods are fixedly connected with one side of the disc respectively, and the upper side of the disc is fixedly connected with the lower sides of a group of evenly distributed lower spherical shells. The device can realize that the box drives a set of drum and reciprocates the slip from top to bottom in reciprocating rotation, can carry out even vibration to the exosome and the reagent in a set of test tube.

However, the device still has certain defect in practical use, and the device is placed into the drum to vibrate when using, but the device can repeatedly reciprocate at the in-process of vibrating the drum, and when the drum reciprocates, because the test tube placed in the drum is not fixed, when the drum reciprocates, the test tube can fly out from the drum due to inertia effect, and not only can the test tube be broken, but also the test tube flies out and hits the staff easily, and the security is relatively poor.

Disclosure of Invention

The utility model aims to provide an oscillation device for separating exosomes, so as to solve the problems in the background technology.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

the vibration device for separating the exosomes comprises a base, wherein four corners of the upper end surface of the base are fixedly connected with limiting slide bars, vibration plates are arranged between the four limiting slide bars, sliding sleeves which are in sliding connection with the limiting slide bars are arranged at the four corners of the vibration plates, a plurality of placing cylinders are fixedly connected with the upper end surface of the vibration plates, side openings are formed in two sides of each of the placing cylinders, strip-shaped holes are formed in the placing cylinders, and fixing components used for fixing test tubes are arranged in the strip-shaped holes;

the base up end is equipped with the drive assembly that is used for driving the up-and-down motion of oscillating plate and shakes.

As a further scheme of the utility model: the fixed subassembly includes the lifter, lifter sliding connection is downthehole at the bar, lifter both sides sliding connection has spacing picture peg, spacing picture peg is close to the position fixedly connected with spacing side piece of lifter, install the extension spring between one side and the lifter that the section of thick bamboo was kept away from to spacing picture peg, still be equipped with in the bar and be used for driving the lifter and reciprocate the lifter.

As still further aspects of the utility model: the lifting assembly comprises mounting seats, the mounting seats are respectively and fixedly connected to the positions at two ends of the strip-shaped holes, a thread bush is arranged at the middle position of the lifting block, a threaded rod is connected between the two mounting seats in a rotating mode, the threaded rod is in threaded connection with the thread bush, and a handle is fixedly connected to the upper end of the threaded rod in a penetrating mode.

As still further aspects of the utility model: one end of the limiting plugboard, which is far away from the placement barrel, is fixedly connected with a pull ring.

As still further aspects of the utility model: the driving assembly comprises rotating seats, the two rotating seats are respectively and fixedly connected to the positions on two sides of the upper end face of the base, a driving shaft is rotationally connected between the two rotating seats, rotating discs are installed at two ends of the driving shaft, the positions, away from the circle center, of the rotating discs are rotationally connected with driving rods, the upper ends of the driving rods are rotationally connected with the oscillating plates, and a power assembly used for driving the driving shaft to rotate is further arranged on the base.

As still further aspects of the utility model: the power assembly comprises a driving gear which is fixedly connected to one end of the driving shaft, a motor is arranged at the position, close to the driving shaft, of the upper end face of the base, a motor gear is arranged at the output end of the motor, and the motor gear is meshed with the driving gear.

As still further aspects of the utility model: rubber pads are respectively stuck to the inner walls of the side openings and the lower end surfaces of the limiting plugboards.

As still further aspects of the utility model: and the upper ends of the limiting slide bars are fixedly connected with limiting caps.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the utility model, the test tube can be fixed through the fixing assembly, so that the test tube can be prevented from flying out in the vibration process, and the safety of the device is greatly improved.

2. The height of the lifting block can be adjusted through the lifting assembly, so that the position of the lifting block can be correspondingly adjusted according to the height of the test tube in actual use, and the device can be suitable for test tubes with different heights, and is more convenient to use.

3. The side opening is arranged, so that the test tube in the side opening can be conveniently taken out when the test tube rack is used, and the condition that the test tube is difficult to take out due to the fact that the depth of the accommodating cylinder is large is avoided.

Drawings

Fig. 1 is a schematic structural view of the present utility model.

Fig. 2 is a schematic structural view of a fixing assembly in the present utility model.

Fig. 3 is a schematic structural view of a driving assembly in the present utility model.

Fig. 4 is a schematic view of a partial structure in the present utility model.

Wherein: 1. a base; 2. a limit slide bar; 3. a sliding sleeve; 4. a placing barrel; 5. an oscillating plate; 6. a driving rod; 7. a rotating disc; 8. a motor gear; 9. a motor; 10. a side opening; 11. a bar-shaped hole; 12. a handle; 13. a lifting block; 14. a pull ring; 15. a threaded rod; 16. a mounting base; 17. limiting plugboards; 18. a tension spring; 19. limiting side blocks; 20. a thread sleeve; 21. a drive gear; 22. a drive shaft; 23. and a rotating seat.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Example 1

Referring to fig. 1-4, in the embodiment of the utility model, an oscillation device for separating exosomes comprises a base 1, wherein four corners of the upper end surface of the base 1 are fixedly connected with limiting slide bars 2, oscillation plates 5 are arranged between the four limiting slide bars 2, the four corners of the oscillation plates 5 are respectively provided with a sliding sleeve 3 which is in sliding connection with the limiting slide bars 2, the upper end surface of the oscillation plates 5 is fixedly connected with a plurality of placing cylinders 4, two sides of each placing cylinder 4 are respectively provided with a side opening 10, each placing cylinder 4 is also provided with a strip-shaped hole 11, and fixing components for fixing test tubes are arranged in the strip-shaped holes 11; rubber pads are respectively stuck to the inner wall of the side opening 10 and the lower end face of the limiting plugboard 17; and the upper ends of the limiting slide bars 2 are fixedly connected with limiting caps.

When using, place the test tube that will vibrate and lay a section of thick bamboo 4 in, then fix through fixed subassembly, it is fixed after accomplishing through drive assembly start lay a section of thick bamboo 4 reciprocate vibrate can, the rubber pad that sets up simultaneously can protect the test tube, avoids getting when putting the test tube, the test tube with lay a section of thick bamboo 4 and take place to collide with.

The fixing assembly comprises a lifting block 13, the lifting block 13 is slidably connected in a bar-shaped hole 11, two sides of the lifting block 13 are slidably connected with limiting insertion plates 17, a limiting side block 19 is fixedly connected to the position, close to the lifting block 13, of the limiting insertion plates 17, a tension spring 18 is arranged between one side, far away from the placing cylinder 4, of the limiting insertion plates 17 and the lifting block 13, and a lifting assembly for driving the lifting block 13 to move up and down is further arranged in the bar-shaped hole 11; one end of the limiting plugboard 17, which is far away from the placement barrel 4, is fixedly connected with a pull ring 14.

When the test tube placing device is used, the pull ring 14 is pulled firstly to enable the limiting plugboard 17 to withdraw from the placing barrel 4, the problem that the test tube is blocked when the test tube is placed is avoided, the pull ring 14 is loosened after the test tube is placed, the limiting plugboard 17 is enabled to be returned to the placing barrel 4 again to limit the test tube, and then the limiting plugboard 17 is pressed onto the test tube through the lifting assembly, so that the test tube is prevented from flying out of the placing barrel 4 in the vibration process.

The lifting assembly comprises mounting seats 16, the mounting seats 16 are respectively and fixedly connected to the positions of two ends of the strip-shaped hole 11, a thread bush 20 is arranged in the middle of the lifting block 13, a threaded rod 15 is rotatably connected between the two mounting seats 16, the threaded rod 15 is in threaded connection with the thread bush 20, and a handle 12 is fixedly connected to the upper end of the threaded rod 15, penetrating out of the mounting seats 16.

During operation, the rotating handle 12 drives the threaded rod 15 to rotate, the threaded rod 15 rotates to drive the lifting block 13 to move up and down, and then the height of the limiting plugboard 17 can be correspondingly adjusted according to the height of the test tube, so that the use is more convenient.

The upper end face of the base 1 is provided with a driving component for driving the oscillating plate 5 to move up and down to oscillate; the driving assembly comprises a rotating seat 23, the two rotating seats 23 are respectively and fixedly connected to the positions on two sides of the upper end face of the base 1, a driving shaft 22 is rotatably connected between the two rotating seats 23, rotating discs 7 are installed at two ends of the driving shaft 22, a driving rod 6 is rotatably connected to the position, away from the circle center, of the rotating discs 7, the upper end of the driving rod 6 is rotatably connected with the oscillating plate 5, and a power assembly for driving the driving shaft 22 to rotate is further arranged on the base 1; when the vibration test tube vibration device works, the driving assembly drives the driving shaft 22 to rotate, the driving shaft 22 rotates to drive the rotating disc 7 to rotate, the rotating disc 7 rotates to drive the driving rod 6 to move, the driving rod 6 moves to drive the vibration plate 5 to move up and down, and then the placing cylinder 4 can be driven to move up and down repeatedly to vibrate the test tube.

Example 2

Referring to fig. 3, the difference from embodiment 1 is that: the power assembly comprises a driving gear 21, the driving gear 21 is fixedly connected to one end of a driving shaft 22, a motor is arranged at the position, close to the driving shaft 22, of the upper end face of the base 1, a motor gear 8 is arranged at the output end of the motor 9, and the motor gear 8 is meshed with the driving gear 21; when the motor is in operation, the output end of the motor 9 rotates to drive the motor gear 8 to rotate, and the motor gear 8 is meshed with the driving gear 21 to realize the rotation of the driving shaft 22.

The working principle of the utility model is as follows: when the test tube placing device is used, the pull ring 14 is pulled firstly to enable the limiting plugboard 17 to withdraw from the placing cylinder 4, the problem that the test tube is blocked when the test tube is placed is avoided, the pull ring 14 is loosened after the test tube is placed, the limiting plugboard 17 is returned to the placing cylinder 4 again to limit the test tube, then the limiting plugboard 17 is pressed on the test tube through the lifting assembly, the test tube is prevented from flying out of the placing cylinder 4 in the vibration process, after the placing is completed, the driving assembly drives the driving shaft 22 to rotate, the driving shaft 22 rotates to drive the rotating disc 7 to rotate, the rotating disc 7 rotates to drive the driving rod 6 to move, the driving rod 6 moves to drive the vibration plate 5 to move up and down, and the placing cylinder 4 can be driven to move up and down repeatedly to vibrate the test tube.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. Although the present disclosure describes embodiments in terms of one embodiment, not every embodiment is provided with only one embodiment, and the description is for clarity only, and those skilled in the art should recognize that the embodiments described in the disclosure may be combined appropriately to form other embodiments that will be understood by those skilled in the art.

Claims

1. The utility model provides an exosome is vibration device for separation, includes base (1), its characterized in that: four corners of the upper end face of the base (1) are fixedly connected with limit sliding rods (2), oscillating plates (5) are arranged among the four limit sliding rods (2), sliding sleeves (3) which are in sliding connection with the limit sliding rods (2) are arranged at the four corners of the oscillating plates (5), a plurality of placing cylinders (4) are fixedly connected with the upper end face of the oscillating plates (5), side openings (10) are formed in two sides of each of the placing cylinders (4), strip-shaped holes (11) are formed in the placing cylinders (4), and fixing components used for fixing test tubes are arranged in the strip-shaped holes (11);

the upper end face of the base (1) is provided with a driving component for driving the oscillating plate (5) to move up and down to oscillate.

2. The vibration device for separating the exosome according to claim 1, wherein the fixing component comprises a lifting block (13), the lifting block (13) is slidably connected in the bar-shaped hole (11), two sides of the lifting block (13) are slidably connected with a limiting insertion plate (17), a limiting side block (19) is fixedly connected to the position of the limiting insertion plate (17) close to the lifting block (13), a tension spring (18) is installed between one side, far away from the placing barrel (4), of the limiting insertion plate (17) and the lifting block (13), and a lifting component for driving the lifting block (13) to move up and down is further arranged in the bar-shaped hole (11).

3. The vibration device for separating the exosome according to claim 2, wherein the lifting assembly comprises mounting seats (16), the mounting seats (16) are respectively and fixedly connected at the positions of two ends of the strip-shaped hole (11), a threaded sleeve (20) is arranged at the middle position of the lifting block (13), a threaded rod (15) is rotationally connected between the two mounting seats (16), the threaded rod (15) is in threaded connection with the threaded sleeve (20), and a handle (12) is fixedly connected at the position of the upper end of the threaded rod (15) penetrating out of the mounting seat (16).

4. The vibration device for separating the exosomes according to claim 2, wherein a pull ring (14) is fixedly connected to one end of the limiting plugboard (17) far away from the placement barrel (4).

5. The vibration device for separating exosomes according to claim 1, wherein the driving assembly comprises rotating seats (23), the two rotating seats (23) are fixedly connected to the positions on two sides of the upper end face of the base (1) respectively, a driving shaft (22) is rotatably connected between the two rotating seats (23), rotating discs (7) are mounted at two ends of the driving shaft (22), driving rods (6) are rotatably connected to positions, away from the circle center, of the rotating discs (7), the upper ends of the driving rods (6) are rotatably connected with the vibration plates (5), and a power assembly for driving the driving shaft (22) to rotate is further arranged on the base (1).

6. The vibration device for separating exosomes according to claim 5, wherein the power assembly comprises a driving gear (21), the driving gear (21) is fixedly connected to a position at one end of a driving shaft (22), a motor (9) is installed at a position, close to the driving shaft (22), of the upper end face of the base (1), a motor gear (8) is installed at the output end of the motor (9), and the motor gear (8) is meshed with the driving gear (21).

7. The vibration device for separating the exosomes according to claim 2, wherein rubber pads are respectively stuck to the inner wall of the side opening (10) and the lower end surface of the limiting plugboard (17).

8. The vibration device for separating exosomes according to claim 1, wherein the upper ends of the limit sliding rods (2) are fixedly connected with limit caps.