CN219385167U

CN219385167U - Cell culture device

Info

Publication number: CN219385167U
Application number: CN202223559974.2U
Authority: CN
Inventors: 张丽; 王昭; 谢艳
Original assignee: Luoyang Orthopedic Traumatological Hospital of Henan Orthopedic Hospital
Current assignee: Luoyang Orthopedic Traumatological Hospital of Henan Orthopedic Hospital
Priority date: 2022-12-30
Filing date: 2022-12-30
Publication date: 2023-07-21
Anticipated expiration: 2032-12-30

Abstract

The utility model provides a cell culture device, which comprises a box body, wherein a baffle plate is arranged in the box body, a plurality of through holes for placing culture dishes are formed in the baffle plate, a temperature sensor is arranged at the bottom of the baffle plate, a heater is arranged at the bottom of the inner side of the box body, a first annular pipe and a second annular pipe are arranged at the outer side of the box body and correspond to the lower part of the baffle plate, a plurality of outlets extending to the inner side of the box body are formed in the first annular pipe and the second annular pipe respectively, the first annular pipe and the second annular pipe are communicated through a connecting pipe, and a reflux pump is arranged on the connecting pipe; a box cover is arranged above the box body, and a liquid suction pipe is arranged on the box cover in a penetrating manner corresponding to each through hole; the top of the box cover is provided with a sampling tube, a piston rod is arranged in the sampling tube, the bottom of the sampling tube is provided with an inlet and an outlet, and the inlet and the outlet are respectively connected with a first sealing cover and a second sealing cover through hoses. The cell culture device not only facilitates high-efficiency sampling and observation of cells, but also facilitates regulation of cell culture temperature, and has simple and practical structural design.

Description

Cell culture device

Technical Field

The utility model belongs to the technical field of cell culture, and particularly relates to a cell culture device.

Background

In bioengineering technology, cell culture is an indispensable process, and cell culture is generally performed by placing cells in a culture dish and then placing the culture dish in an incubator under a certain temperature condition. The existing incubator has the following problems in the use process: 1. the cells are often required to be sampled and observed during the culture period, the conventional sampling mode is to take the culture dish out of the incubator, then to extract part of the cells by using a suction pipe and put the extracted cells under a microscope to observe the growth condition of the cells, and the sampling mode can easily cause the culture dish to be polluted by the outside and influence the culture effect; 2. the existing incubator generally places the culture dish on a certain shelf in the incubator during the use process, so that the culture dish is not convenient to fix; 3. the temperature inside the incubator is not convenient to flexibly adjust.

There is therefore a need for a new cell culture apparatus to solve the above problems.

Disclosure of Invention

The utility model aims at overcoming the defects of the prior art, and provides a cell culture device, which comprises the following specific scheme:

the cell culture device comprises a box body, wherein a partition plate is arranged in the box body, a plurality of through holes for placing culture dishes are formed in the partition plate, a temperature sensor is arranged at the bottom of the partition plate, a heater is arranged at the bottom of the inner side of the box body, a first annular pipe and a second annular pipe are arranged below the partition plate, which correspond to the lower side of the partition plate, a plurality of outlets extending to the inner side of the box body are formed in the first annular pipe and the second annular pipe, the first annular pipe and the second annular pipe are communicated through connecting pipes, and a reflux pump is arranged on the connecting pipes; a water filling port is also arranged on the second annular pipe; a box cover is arranged above the box body, a liquid suction pipe is arranged on the box cover corresponding to each through hole in a penetrating mode, a one-way valve is arranged in the liquid suction pipe, and a cover plate aiming at the culture dish is arranged on the liquid suction pipe; the top of the box cover is provided with a supporting frame, the supporting frame is provided with a sampling tube, a piston rod is arranged in the sampling tube, the bottom of the sampling tube is provided with an inlet and an outlet, the inlet and the outlet are respectively connected with a first sealing cover and a second sealing cover through hoses, the first sealing cover is used for being in sealing connection with the top end of the liquid suction tube, the second sealing cover is used for being in sealing connection with a sampling test tube, and the inlet and the outlet are both provided with one-way valves; the temperature sensor, the heater and the reflux pump are electrically connected with a controller arranged outside the box body; the bottom of the box body is provided with a plurality of elastic supporting legs, and the bottom of the elastic supporting legs is provided with universal wheels with locking mechanisms.

Based on the above, the bottom of the inner side of the box body is also provided with a liquid level meter electrically connected with the controller.

Based on the above, the case cover is further provided with a UV lamp electrically connected with the controller.

Based on the above, the bottom of the box body is provided with a storage battery for supplying power to the whole cell culture device.

Based on the above, the box body and the box cover are respectively provided with an upper edge and a lower edge which correspond to each other, the upper edge and the lower edge are provided with a plurality of threaded through holes which correspond to each other, and the box body and the box cover are fixedly installed together through butterfly bolts which correspond to the threaded through holes.

Based on the above, the case cover is provided with a handle.

Compared with the prior art, the utility model has substantial characteristics and progress, and particularly has the following advantages:

1. according to the utility model, the heater is arranged at the bottom of the inner side of the box body, the first annular pipe and the second annular pipe are arranged below the corresponding partition plate outside the box body, a plurality of outlets extending to the inner side of the box body are arranged on the first annular pipe and the second annular pipe, the first annular pipe and the second annular pipe are communicated through the connecting pipe, and the reflux pump is arranged on the connecting pipe; this structural design is convenient for carry out quick even heating up to the water in the box and handles, guarantees the cell culture environment.

2. According to the utility model, the liquid suction pipe is arranged on the box cover in a penetrating way corresponding to each through hole, the liquid suction pipe is internally provided with the one-way valve, and the liquid suction pipe is provided with the cover plate aiming at the culture dish; the top of the box cover is provided with a supporting frame, the supporting frame is provided with a sampling tube, a piston rod is arranged in the sampling tube, the bottom of the sampling tube is provided with an inlet and an outlet, the inlet and the outlet are respectively connected with a first sealing cover and a second sealing cover through hoses, the first sealing cover is used for being in sealing connection with the top end of a liquid suction pipe, the second sealing cover is used for being in sealing connection with a sampling test tube, and the inlet and the outlet are respectively provided with one-way valves; the structure design is not only convenient for extracting and observing cells cultured in the culture dish in a closed environment and avoiding the cells in the culture dish from being polluted by the external environment, but also the design of the upper cover plate of the liquid suction pipe is convenient for fixing the culture dish and preventing the culture solution from overflowing.

Drawings

Fig. 1 is a schematic view of the overall structure of the present utility model.

In the figure: 1. a case; 2. a case cover; 3. butterfly bolts; 4. a partition plate; 5. a culture dish; 6. a heater; 7. a first annular tube; 8. a second annular tube; 9. a water filling port; 10. a connecting pipe; 11. a reflux pump; 12. a liquid level gauge; 13. a temperature sensor; 14. a liquid suction pipe; 15. a cover plate; 16. a one-way valve; 17. a support frame; 18. a sampling tube; 18-1, a piston rod; 19. an inlet; 20. a first sealing cover; 21. an outlet; 22. a second sealing cover; 23. a handle; 24. a storage battery; 25. an elastic support leg; 26. a universal wheel; 27. a controller; uv lamp tube.

Detailed Description

The technical scheme of the utility model is further described in detail through the following specific embodiments.

Examples

As shown in fig. 1, the utility model provides a cell culture device, which comprises a box body 1, wherein a partition board 4 is arranged in the box body 1, a plurality of through holes for placing a culture dish 5 are formed in the partition board 4, a temperature sensor 13 is arranged at the bottom of the partition board 4, a heater 6 is arranged at the bottom of the inner side of the box body 1, a first annular pipe 7 and a second annular pipe 8 are arranged at the periphery of the box body 1 and correspond to the lower part of the partition board 4, a plurality of outlets 21 extending into the box body 1 are formed in the first annular pipe 7 and the second annular pipe 8, the first annular pipe 7 and the second annular pipe 8 are communicated through a connecting pipe 10, and a reflux pump 11 is arranged on the connecting pipe 10; a water filling port 9 is also arranged on the second annular pipe 8; a case cover 2 is arranged above the case body 1, a liquid suction pipe 14 is arranged on the case cover 2 corresponding to each through hole in a penetrating way, a one-way valve 16 is arranged in the liquid suction pipe 14, and a cover plate 15 aiming at the culture dish 5 is arranged on the liquid suction pipe 14; the top of the box cover 2 is provided with a supporting frame 17, the supporting frame 17 is provided with a sampling tube 18, a piston rod 18-1 is arranged in the sampling tube 18, the bottom of the sampling tube 18 is provided with an inlet 19 and an outlet 21, the inlet 19 and the outlet 21 are respectively connected with a first sealing cover 20 and a second sealing cover 22 through hoses, the first sealing cover 20 is used for being in sealing connection with the top end of the liquid suction tube 14, the second sealing cover 22 is used for being in sealing connection with a sampling test tube, and the positions of the inlet 19 and the outlet 21 are respectively provided with a one-way valve 16; the temperature sensor 13, the heater 6 and the reflux pump 11 are electrically connected with a controller 27 arranged outside the box body 1; the bottom of the box body 1 is provided with a plurality of elastic supporting legs 25, and universal wheels 26 with locking mechanisms are arranged at the bottoms of the elastic supporting legs 25.

In order to monitor the water level in the tank 1, a level gauge 12 electrically connected to the controller 27 is further disposed at the bottom of the inner side of the tank 1.

In order to facilitate the disinfection and sterilization of the interior of the case 1, a UV lamp 28 electrically connected to the controller 27 is further provided on the case cover 2.

To ensure the suitability of the culture device for the environment, a battery 24 is provided at the bottom of the housing 1 to supply power to the whole cell culture device.

The box body 1 and the box cover 2 are respectively provided with an upper edge and a lower edge which correspond to each other, the upper edge and the lower edge are provided with a plurality of threaded through holes which correspond to each other, and the box body 1 and the box cover 2 are fixedly installed together through butterfly bolts 3 which correspond to the threaded through holes.

To facilitate the movement of the cover 2, a handle 23 is provided on the cover 2.

The utility model has the specific working principle that: first, the UV lamp 28 is turned on to sterilize the inside of the cabinet 1.

Then placing a culture dish 5 containing cells and culture solution into the through holes on the partition board 4, fixing the box cover 2 on the box body 1, setting the temperature range of cell culture by the controller 27, adding water into the box body 1 through the water filling port 9, and observing the water level through the liquid level meter 12; after the water quantity reaches a certain degree, the heating button is started, and the heater 6 and the reflux pump 11 work simultaneously until the temperature rises to a set range.

When the temperature inside the tank 1 is lower than the set temperature range, the controller 27 automatically activates the heater 6 and the reflux pump 11.

When the cells in the culture dish 5 need to be sampled and observed, the first sealing cover 20 is covered on the top end of the corresponding liquid suction pipe 14, the second sealing cover 22 is covered on the end part which is not shown in the sampling test tube diagram, then the piston rod 18-1 is pulled for sampling, and then the piston rod 18-1 is reset to discharge the cells into the test tube.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model and not for limiting the same; while the utility model has been described in detail with reference to the preferred embodiments, those skilled in the art will appreciate that: modifications may be made to the specific embodiments of the present utility model or equivalents may be substituted for part of the technical features thereof; without departing from the spirit of the utility model, it is intended to cover the scope of the utility model as claimed.

Claims

1. A cell culture apparatus, characterized in that: the incubator comprises a incubator body (1), wherein a baffle plate (4) is arranged inside the incubator body (1), a plurality of through holes for placing culture dishes (5) are formed in the baffle plate (4), a temperature sensor (13) is arranged at the bottom of the baffle plate (4), a heater (6) is arranged at the bottom of the inner side of the incubator body (1), a first annular pipe (7) and a second annular pipe (8) are arranged below the baffle plate (4) corresponding to the periphery of the incubator body (1), a plurality of outlets (21) extending into the incubator body (1) are formed in the first annular pipe (7) and the second annular pipe (8), the first annular pipe (7) and the second annular pipe (8) are communicated through a connecting pipe (10), and a reflux pump (11) is arranged on the connecting pipe (10); a water filling port (9) is also arranged on the second annular pipe (8); a box cover (2) is arranged above the box body (1), a liquid suction pipe (14) is arranged on the box cover (2) corresponding to each through hole in a penetrating mode, a one-way valve (16) is arranged in the liquid suction pipe (14), and a cover plate (15) aiming at the culture dish (5) is arranged on the liquid suction pipe (14); the top of the box cover (2) is provided with a supporting frame (17), the supporting frame (17) is provided with a sampling tube (18), a piston rod (18-1) is arranged in the sampling tube (18), the bottom of the sampling tube (18) is provided with an inlet (19) and an outlet (21), the inlet (19) and the outlet (21) are respectively connected with a first sealing cover (20) and a second sealing cover (22) through hoses, the first sealing cover (20) is used for being in sealing connection with the top end of the liquid suction tube (14), the second sealing cover (22) is used for being in sealing connection with a sampling test tube, and the positions of the inlet (19) and the outlet (21) are respectively provided with one-way valves (16); the temperature sensor (13), the heater (6) and the reflux pump (11) are electrically connected with a controller (27) arranged outside the box body (1); the bottom of the box body (1) is provided with a plurality of elastic supporting legs (25), and the bottom of the elastic supporting legs (25) is provided with universal wheels (26) with locking mechanisms.

2. The cell culture apparatus of claim 1, wherein: the bottom of the inner side of the box body (1) is also provided with a liquid level meter (12) which is electrically connected with the controller (27).

3. The cell culture apparatus of claim 1, wherein: and the box cover (2) is also provided with a UV lamp tube (28) electrically connected with the controller (27).

4. The cell culture apparatus of claim 1, wherein: the bottom of the box body (1) is provided with a storage battery (24) for supplying power to the whole cell culture device.

5. The cell culture apparatus of claim 1, wherein: the novel anti-theft box is characterized in that an upper edge and a lower edge which correspond to each other are respectively arranged on the box body (1) and the box cover (2), a plurality of threaded through holes which correspond to each other are formed in the upper edge and the lower edge, and the box body (1) and the box cover (2) are fixedly installed together through butterfly bolts (3) which correspond to the threaded through holes.

6. The cell culture apparatus of claim 1, wherein: the case cover (2) is provided with a handle (23).