CN219320086U - Spherical brown algae cyst body inherent optical measurement system based on spectrophotometer - Google Patents

Spherical brown algae cyst body inherent optical measurement system based on spectrophotometer Download PDF

Info

Publication number
CN219320086U
CN219320086U CN202222559261.XU CN202222559261U CN219320086U CN 219320086 U CN219320086 U CN 219320086U CN 202222559261 U CN202222559261 U CN 202222559261U CN 219320086 U CN219320086 U CN 219320086U
Authority
CN
China
Prior art keywords
spectrophotometer
cuvette
brown algae
integrating sphere
cyst
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202222559261.XU
Other languages
Chinese (zh)
Inventor
李薛
林供
商少凌
李忠平
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xiamen University
Original Assignee
Xiamen University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xiamen University filed Critical Xiamen University
Priority to CN202222559261.XU priority Critical patent/CN219320086U/en
Application granted granted Critical
Publication of CN219320086U publication Critical patent/CN219320086U/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The utility model discloses a spherical brown algae cyst body inherent optical measurement system based on a spectrophotometer, which comprises an absorption measurement device, an attenuation measurement device and a back scattering measurement device; the absorption measurement device comprises a first color comparison dish and a first spectrophotometer to detect absorbance of the spherical brown algae capsule; the attenuation measuring device comprises a second cuvette, a black slit plate and a second spectrophotometer to detect the attenuation degree of the spherical brown algae cyst; the back-scattering measurement device comprises a third cuvette and a third spectrophotometer to detect the reflectivity of the spherical brown algae cysts. The utility model can effectively protect the structure of the spherical brown algae cyst and simultaneously can measure the inherent optical characteristics (absorption, attenuation and backscattering) of the spherical brown algae cyst.

Description

Spherical brown algae cyst body inherent optical measurement system based on spectrophotometer
Technical Field
The utility model relates to the technical field of spherical brown algae cyst body measurement, in particular to a spherical brown algae cyst body inherent optical measurement system based on a spectrophotometer.
Background
Spherical brown algae (P.globosa) has frequently exploded algal bloom in coastal areas of China in recent years, and is one of main algae of Harmful Algal Bloom (HABs) endangering coasts of China. The algal bloom takes the large colloid capsule as the main material, and the maximum capsule diameter is even more than 3cm, so that serious threat is generated to the safety of the cold source of the coastal nuclear power. The development of a rapid and efficient monitoring means for the algal bloom, in particular a remote sensing monitoring technology with the advantages of large-scale synchronization, no need of sampling measurement and the like, is certainly of great significance. The inherent optical characteristics of the spherical brown algae cysts are important basis for analyzing the remote sensing mechanism of the spherical brown algae cysts, but are limited by the special life form of the spherical brown algae, the uneven space-time distribution of the cysts, the low indoor culture cyst rate and other reasons, so that the understanding of the remote sensing mechanism of the spherical brown algae cysts is quite deficient. The traditional quantitative filtering membrane technology and the commercial inherent optical measuring instrument can lead to the breakage of the capsule body during measurement, thereby causing measurement errors.
Disclosure of Invention
The utility model mainly aims to provide a spherical brown algae cyst body inherent optical measurement system based on a spectrophotometer, which can effectively protect the spherical brown algae cyst body structure and simultaneously obtain the absorption, attenuation and back scattering optical characteristics of the spherical brown algae cyst body.
The utility model adopts the following technical scheme:
a spectrophotometer-based spherical brown algae capsule intrinsic optical measurement system comprising:
an absorption measurement device comprising a first cuvette and a first spectrophotometer; the first spectrophotometer includes a first integrating sphere detector; a first spherical brown algae cyst body sample is placed in the first color comparison dish; the first colorimetric ware is arranged in a first integrating sphere detector, and the first integrating sphere detector measures the absorbance of the spherical brown algae capsule;
the attenuation measuring device comprises a second cuvette, a black slit plate and a second spectrophotometer; the second spectrophotometer includes a second integrating sphere detector; a second spherical brown algae cyst body sample is placed in the second cuvette; the black slit plate is arranged between the second cuvette and the second integrating sphere detector, and the second integrating sphere detector is used for measuring the attenuation degree of the spherical brown algae cyst;
a back-scatter measurement device comprising a third cuvette and a third spectrophotometer; the third spectrophotometer includes a third integrating sphere detector; a third cuvette in which a third sample of spherical brown algae cysts is placed is arranged at the reflective window of a third integrating sphere detector that measures the reflectivity of the spherical brown algae cysts.
Preferably, the absorption measurement device further comprises: a fixing frame; the first colorimetric ware is mounted on the fixing frame.
Preferably, the first cuvette is a cylindrical quartz cuvette; the diameter of the first cuvette is 2.5cm, and the optical path is 4cm.
Preferably, the second spectrophotometer further comprises: a sample bin; the second cuvette is disposed within the sample compartment.
Preferably, the forward opening angle of the black slit plate is not more than 0.23 °.
Preferably, the third cuvette is a quartz cuvette; the diameter of the third cuvette is 2.5cm, and the optical path is 10cm.
Preferably, the front end of the third cuvette is provided with a convex hemispherical shape, and the rear end of the third cuvette is provided with black aluminum foil paper.
Preferably, the backscatter measurement device further comprises a base; the third cuvette is mounted on the base.
Preferably, the backscatter measurement device further comprises a back cover; the rear cover is arranged at the tail end of the third integrating sphere detector and covers the base; the inner layer of the rear cover is arranged into a honeycomb net structure, and the inner surface of the rear cover is coated with a black coating.
Preferably, the diameter of the first integrating sphere, the diameter of the second integrating sphere and the diameter of the third integrating sphere are all 15cm.
Compared with the prior art, the utility model has the following beneficial effects:
(1) According to the utility model, the fixing frame fixed with the cylindrical quartz cuvette with the diameter of 2.5cm and the optical path of 4cm is placed into the integrating sphere detector with the first spectrophotometer with the diameter of 15cm to measure the absorption coefficient of the spherical brown algae cyst;
(2) According to the utility model, the black slit plate is added in front of the integrating sphere detector of the second spectrophotometer for measuring the attenuation coefficient of the spherical brown algae, and the forward opening angle is reduced to be not more than 0.23 DEG, so that the detector can only obtain transmitted light and scattered light within 0.23 DEG, the measurement error is greatly reduced, and the measurement precision is improved;
(3) According to the utility model, the third cuvette is placed in the reflection window of the integrating sphere detector of the third spectrophotometer for backward scattering measurement, the front end of the third cuvette is provided with the convex hemispherical shape, the rear end of the third cuvette is provided with the black aluminum foil paper, the tail end of the integrating sphere of the third spectrophotometer is provided with the rear cover, the inner layer of the rear cover is provided with the honeycomb net structure, and the inner surface of the rear cover is coated with the black coating, so that the measurement has high absorption and low reflectivity, and the measurement precision is improved.
The foregoing description is only an overview of the present utility model, and is intended to provide a more clear understanding of the technical means of the present utility model, so that it may be carried out in accordance with the teachings of the present specification, and to provide a more complete understanding of the above and other objects, features and advantages of the present utility model, as exemplified by the following detailed description.
The above and other objects, advantages and features of the present utility model will become more apparent to those skilled in the art from the following detailed description of the specific embodiments of the present utility model when taken in conjunction with the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram of an absorption measurement device according to an embodiment of the present utility model;
FIG. 2 is a schematic diagram of a first cuvette and a holder according to an embodiment of the present utility model;
FIG. 3 is a schematic diagram of an attenuation measuring device according to an embodiment of the present utility model;
fig. 4 is a schematic structural view of a black slit plate according to an embodiment of the present utility model;
FIG. 5 is a schematic view illustrating a structure of a backscatter measurement device according to an embodiment of the present utility model
FIG. 6 is a schematic diagram of a third cuvette and a base according to an embodiment of the present utility model;
fig. 7 is a schematic structural view of a rear cover of an integrating sphere of a third spectrophotometer according to an embodiment of the present utility model.
Detailed Description
The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model; it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments, and that all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments in the present utility model are within the protection scope of the present utility model.
In the description of the present utility model, it should be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "inner", "outer", "top/bottom/front/rear", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of description and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "provided," "engaged/connected," "connected," and the like are to be construed broadly, and for example, "connected" may be a fixed connection, may be a detachable connection, or may be an integral connection, may be a mechanical connection, may be an electrical connection, may be a direct connection, may be an indirect connection via an intermediary, may be a communication between two elements, and for one of ordinary skill in the art, the specific meaning of the terms in this disclosure may be understood in a specific case.
Referring to fig. 1 to 7, the utility model provides a spectrophotometer-based spherical brown algae cyst intrinsic optical measurement system, comprising:
an absorption measurement device 1 comprising a first cuvette and a first spectrophotometer; the first spectrophotometer includes a first integrating sphere detector; a first spherical brown algae cyst body sample is placed in the first color comparison dish; the first colorimetric ware is arranged in a first integrating sphere detector, and the first integrating sphere detector measures the absorbance of the spherical brown algae capsule;
an attenuation measuring device 2 including a second cuvette, a black slit plate, and a second spectrophotometer; the second spectrophotometer includes a second integrating sphere detector; a second spherical brown algae cyst body sample is placed in the second cuvette; the black slit plate is arranged between the second cuvette and the second integrating sphere detector, and the second integrating sphere detector is used for measuring the attenuation degree of the spherical brown algae cyst;
a backscatter measurement device 3 comprising a third cuvette and a third spectrophotometer; the third spectrophotometer includes a third integrating sphere detector; a third cuvette in which a third sample of spherical brown algae cysts is placed is arranged at the reflective window of a third integrating sphere detector that measures the reflectivity of the spherical brown algae cysts.
It should be noted that the first, second and third spherical brown algae cyst samples may be understood as the same sample divided into three for measurement. The absorption measurement device 1 and the attenuation measurement device 2 are to perform measurement simultaneously, so that the first spectrophotometer and the second spectrophotometer are different spectrophotometers, and the third spectrophotometer may be the same spectrophotometer as the first spectrophotometer and the second spectrophotometer, or different spectrophotometers may be used. In this embodiment, the first spectrophotometer, the second spectrophotometer, and the third spectrophotometer may employ Lambda 950, lambda 850, or the like. The utility model designs a spherical brown algae cyst intrinsic optical measurement system based on a spectrophotometer based on the characteristics of the spherical brown algae cyst, so that the absorption, attenuation and backward scattering optical characteristics of the spherical brown algae cyst can be obtained while the structure of the spherical brown algae cyst is effectively protected, the measurement error is reduced, and the measurement precision is improved.
Specifically, referring to fig. 1 and 2, in this embodiment, the first cuvette 10 is a cylindrical quartz cuvette, the first cuvette 10 is fixed by a fixing frame 11, and during absorption measurement, the fixing frame 11 with the first cuvette 10 fixed thereon is placed in the first integrating sphere detector 12 for absorption measurement. Specifically, first cuvette 10 may be placed in the center of first integrating sphere detector 12. Referring to fig. 3, the light source 13 sequentially passes through the filter 14, the slit 15, the first cuvette 10 and the reflecting plate 16, and the first integrating sphere detector 12 compares the absorbance value OD s Measurements were made. The light source 13, the filter 14, the slit 15 and the reflection plate 16 of the first spectrophotometer may be existing components of the first spectrophotometer. According to the opening size of the first integrating sphere detector 12, the utility model prepares a cylindrical quartz cuvette with the diameter of 2.5cm and the optical path of 4cm so as to just put the cuvetteThe integrated ball is filled, and the spherical brown algae cysts can be accommodated, so that the absorption measurement of the spherical brown algae cysts can be better applicable while the structure of the spherical brown algae cysts is not damaged. In this embodiment, the diameter of the first integrating sphere detector 12 is 15cm.
In FIG. 1, I 0 Representing incident light; i a Representing Absorption; i s Representing Scattering; i t Representing transmitted light Transmitted light.
The attenuation coefficient can be obtained by measuring the light beam transmittance by a spectrophotometer, and since the forward scattering of suspended algae cells accounts for a large total scattering specific gravity, in order to reduce the measurement error of the attenuation coefficient, the detection distance is increased and the slit is reduced as much as possible before the integrating sphere detector to prevent the forward scattering from being detected by the detector. Referring to fig. 3 and 4, in this embodiment, a black slit plate 21 is added in front of the second integrating sphere detector 20, and the forward open angle is reduced to 0.23 °, so that the detector can only obtain transmitted light and scattered light within 0.23 °, thereby greatly reducing measurement errors and improving measurement accuracy. In order to ensure the validity of the measurement, in this embodiment, the attenuation measurement and the absorption measurement need to be performed simultaneously.
Referring to fig. 3, a light source 22 of the second spectrophotometer sequentially passes through a filter 23, a slit 24, a sample chamber 25 (a second cuvette is provided therein, which is not shown in the figure, and an existing cuvette may be used for the second cuvette in the attenuation measurement), and a black slit plate 21, and the attenuation coefficient is measured by a second integrating sphere detector 20. The light source 22, filter 23, slit 24 and sample compartment 25 of the second spectrophotometer may be existing components of the second spectrophotometer.
In FIG. 3, I 0 Representing incident light; i a Representing Absorption; i s Representing Scattering; i t Representing transmitted light Transmitted light.
Referring to fig. 5-7, in the case of the spherical brown algae capsule backscattering measurement, the third cuvette 30 is placed in the reflection window of the integrating sphere detector of the dual-path uv-vis spectrophotometer (e.g., lambda 850) to measure the backscattering flux.
In this embodiment, the third cuvette 30 is a quartz cuvette, the diameter of the quartz cuvette is 2.5cm, and the optical path is 10cm; the front end of the third cuvette 30 is provided with a convex hemispherical shape, and the rear end of the third cuvette 30 is provided with black aluminum foil paper; the diameter of the third integrating sphere detector 33 is 15cm. The third cuvette 30 is arranged on the base 31; the integrating sphere end of the third spectrophotometer is provided with a rear cover 32 covering the base 31, the inner layer of the rear cover 32 is provided in a honeycomb net structure and the inner surface is coated with a black coating. In addition, the material of back lid skin can be duralumin or stainless steel, the material of back lid inlayer can be aluminium.
Referring to fig. 5, the light source 34 of the third spectrophotometer sequentially passes through the filter 35, the slit 36 and the third cuvette 30, and the third integrating sphere detector 33 measures the reflectivity. The light source 34, filter 35 and slit 36 of the third spectrophotometer may be existing components of the third spectrophotometer.
In FIG. 5, I 0 Representing the Incident light, incoden light; i a Representing Absorption; i bb Representing back-scattered flux scattering Backscattering flux; i fb Representing back-scattered flux scattering Forward scattering flux; i t Representing transmitted light Transmitted light; i r Representing Reflected flux
Errors in the backscatter measurements may be mainly due to: (1) instrument error; (2) specular reflection from the front end of the quartz cuvette; (3) specular reflection at the rear end of the quartz cuvette. The performance of the integrating sphere rear cover customized by the embodiment is superior to that of the original integrating sphere rear cover, the integrating sphere rear cover has high absorption and low reflectivity, and the average reflectivity of the new and old systems in the range of 400-800nm is 0.06% and 0.14% respectively. The measurement result of the sample filtrate with the thickness of 0.2 μm is removed, so that the influence of the reflection of the front end mirror surface of the quartz cuvette can be offset to the greatest extent. Whereas the back end mirror reflection of the cuvette may be reduced by placing an ultra low reflectivity black aluminum foil (Thorlabs, inc.) at the back end of the quartz cuvette during measurement.
The above description is only of the preferred embodiments of the present utility model; the scope of the utility model is not limited in this respect. Any person skilled in the art, within the technical scope of the present disclosure, may apply to the present utility model, and the technical solution and the improvement thereof are all covered by the protection scope of the present utility model.

Claims (10)

1. A spectrophotometer-based spherical brown algae capsule intrinsic optical measurement system, comprising:
an absorption measurement device comprising a first cuvette and a first spectrophotometer; the first spectrophotometer includes a first integrating sphere detector; a first spherical brown algae cyst body sample is placed in the first color comparison dish; the first color comparison dish is arranged in the first integrating sphere detector;
the attenuation measuring device comprises a second cuvette, a black slit plate and a second spectrophotometer; the second spectrophotometer includes a second integrating sphere detector; a second spherical brown algae cyst body sample is placed in the second cuvette; the black slit plate is arranged between the second cuvette and the second integrating sphere detector;
a back-scatter measurement device comprising a third cuvette and a third spectrophotometer; the third spectrophotometer includes a third integrating sphere detector; a third cuvette with a third aliquot of the spherical brown algae cyst body sample was placed in the reflective window of a third integrating sphere detector.
2. The spectrophotometer-based spherical brown algae capsule inherent optical measurement system of claim 1, wherein the absorption measurement device further comprises: a fixing frame; the first colorimetric ware is mounted on the fixing frame.
3. The spectrophotometer-based spherical brown algae cyst intrinsic optical measurement system of claim 1, wherein the first cuvette is a cylindrical quartz cuvette; the diameter of the first cuvette is 2.5cm, and the optical path is 4cm.
4. The spectrophotometer-based spherical brown algae capsule inherent optical measurement system of claim 1, wherein the second spectrophotometer further comprises: a sample bin; the second cuvette is disposed within the sample compartment.
5. The spectrophotometer-based spherical brown algae capsule inherent optical measurement system of claim 1, wherein the forward open angle of the black slit plate is no greater than 0.23 °.
6. The spectrophotometer-based spherical brown algae cyst intrinsic optical measurement system of claim 1, wherein the third cuvette is a quartz cuvette; the diameter of the third cuvette is 2.5cm, and the optical path is 10cm.
7. The spectrophotometer-based spherical brown algae cyst intrinsic optical measurement system of claim 1, wherein the front end of the third cuvette is provided with a convex hemispherical shape, and the rear end of the third cuvette is provided with black aluminum foil paper.
8. The spectrophotometer-based spherical brown algae capsule inherent optical measurement system of claim 1, wherein the backscatter measurement device further comprises a base; the third cuvette is mounted on the base.
9. The spectrophotometer-based spherical brown algae capsule inherent optical measurement system of claim 8, wherein the backscatter measurement device further comprises a back cover; the rear cover is arranged at the tail end of the third integrating sphere detector and covers the base; the inner layer of the rear cover is arranged into a honeycomb net structure, and the inner surface of the rear cover is coated with a black coating.
10. The spectrophotometer-based spherical brown algae cyst intrinsic optical measurement system of claim 1, wherein the diameter of the first integrating sphere, the diameter of the second integrating sphere, and the diameter of the third integrating sphere are all 15cm.
CN202222559261.XU 2022-09-27 2022-09-27 Spherical brown algae cyst body inherent optical measurement system based on spectrophotometer Active CN219320086U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202222559261.XU CN219320086U (en) 2022-09-27 2022-09-27 Spherical brown algae cyst body inherent optical measurement system based on spectrophotometer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202222559261.XU CN219320086U (en) 2022-09-27 2022-09-27 Spherical brown algae cyst body inherent optical measurement system based on spectrophotometer

Publications (1)

Publication Number Publication Date
CN219320086U true CN219320086U (en) 2023-07-07

Family

ID=87004014

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202222559261.XU Active CN219320086U (en) 2022-09-27 2022-09-27 Spherical brown algae cyst body inherent optical measurement system based on spectrophotometer

Country Status (1)

Country Link
CN (1) CN219320086U (en)

Similar Documents

Publication Publication Date Title
CN104596990B (en) Double channel optical fiber turbidimetry method and sensor
US4711126A (en) Sensor for the measurement of the refractive index of a fluid and/or phase boundary between two fluids by means of visible or invisible light
CN105424631B (en) A kind of hypersensitivity nitrogen oxides measuring system based on UV, visible light wave band absorption spectrum
EP2238233B1 (en) Detecting and counting bacteria suspended in biological fluids
Zaneveld et al. Reflective-tube absorption meter
JPH01501505A (en) Characteristics monitoring device for thin films on substrates
CN103439233A (en) Flue dust concentration detection system
CN105866091A (en) Portable trace explosive detector
CN219320086U (en) Spherical brown algae cyst body inherent optical measurement system based on spectrophotometer
CN115773817A (en) Liquid color space RGB value detection device and detection method and application thereof
CN209513613U (en) A kind of integrating sphere detection device
CN201417244Y (en) Laser concentration sensor
CN109470636B (en) ATR total reflection prism
CN107894395A (en) A kind of Aerosol Extinction Coefficients measuring method based on Research on Cavity Ring Down Spectroscopy
CN109799204A (en) A kind of low strength COD measuring device based on spectroscopic methodology
CN202794024U (en) Sample cell used for measuring molecular spectral absorption
CN115684045A (en) Spectrophotometer-based method and system for inherent optical measurement of spherical phaeocystis fuscus capsule
CN106197953B (en) A kind of universal laser gyro reflecting mirror on-line measurement device
CN209309685U (en) A kind of explosion-protection equipment of laser detection methane gas leakage
CN209446841U (en) A kind of polarization-maintaining unidirectional optical power detector
CN208383151U (en) A kind of integral type micro-displacement optical fiber sensing probe
CN205785764U (en) Lens transmittance detector
CN217819978U (en) Automatic carbon dioxide concentration detection device based on CRDS
CN211318159U (en) Flat field concave grating for polychromator
CN108489916A (en) A kind of lake algae chlorophyll-a concentration quick monitoring method

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant