CN219279911U

CN219279911U - Immobilized enzyme reaction device

Info

Publication number: CN219279911U
Application number: CN202223570544.0U
Authority: CN
Inventors: 汪娟娟; 周媛; 曹照兵; 翟景付; 刘淑琦; 陈洋
Original assignee: Anhui Redpont Biotechnology Co ltd
Current assignee: Anhui Redpont Biotechnology Co ltd
Priority date: 2022-12-30
Filing date: 2022-12-30
Publication date: 2023-06-30
Anticipated expiration: 2032-12-30

Abstract

The utility model discloses an immobilized enzyme reaction device which comprises a stainless steel support bracket, wherein a kettle body fixing rod is arranged at the upper end of the stainless steel support bracket, a reaction kettle body is connected to the kettle body fixing rod, a long rod thermometer is arranged on the reaction kettle body, an engine is arranged above the reaction kettle body, an output shaft of the engine is connected with a stirring device, a filtering device consisting of stainless steel and a filter membrane is arranged on the inner side of the bottom of the reaction kettle body, a reaction kettle discharge hole is arranged at the bottom of the reaction kettle body, and a material receiving device is connected to the reaction kettle discharge hole. The immobilized enzyme reaction device provided by the utility model has the advantages that the preparation and the catalytic reaction of the immobilized enzyme are carried out in one reaction kettle body, meanwhile, the reaction liquid and the immobilized enzyme can be separated, the next batch of reaction can be directly fed, the reaction efficiency is greatly improved, the experimental operation is convenient, and the post-treatment pressure is reduced.

Description

Immobilized enzyme reaction device

Technical Field

The utility model relates to the technical field of immobilized enzymes, in particular to an immobilized enzyme reaction device.

Background

The prior process of the fusion expression cellulose binding domain immobilized enzyme experiment is to firstly mix enzyme solution after cell disruption and centrifugation with a certain amount of microcrystalline cellulose at 25 ℃ for 1-2 hours with stirring, then remove the enzyme solution through centrifugation, rinse 1-2 times with phosphate buffer solution with the same volume, centrifugally collect microcrystalline cellulose after enzyme solution binding, namely immobilized enzyme, and the collected immobilized enzyme can be directly used for catalytic reaction.

The whole process comprises the steps of microcrystalline cellulose combination, centrifugal filtration, buffer flushing, centrifugal collection, substrate solution combination and catalytic reaction. At present, because no related equipment can collect immobilized enzyme and catalytic reaction into a whole, the immobilized enzyme can be reused for multiple batches, and reaction liquid and the immobilized enzyme cannot be effectively separated when the multiple batches of reactions are carried out, so that the immobilized enzyme needs to be repeatedly collected and then fed, the operation greatly increases the manpower resource cost, and meanwhile, the loss of the immobilized enzyme is also caused to a certain extent.

Disclosure of Invention

The utility model aims to provide an immobilized enzyme reaction device, which is used for carrying out the preparation and the catalytic reaction of immobilized enzyme in a reaction kettle body, separating reaction liquid from immobilized enzyme, directly feeding the next batch of reaction, greatly improving the reaction efficiency, facilitating the experimental operation, reducing the post-treatment pressure and solving the problems in the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

the utility model provides an immobilized enzyme reaction unit, includes the stainless steel support frame, the upper end of stainless steel support frame is provided with the cauldron body dead lever, be connected with the reation kettle body on the dead lever, be provided with the stock thermometer on the reation kettle body, the top of the reation kettle body is provided with the engine, the output shaft of engine has agitating unit, agitating unit is located the reation kettle body, the inboard of reation kettle body bottom is provided with the filter equipment who comprises stainless steel and filter membrane, the bottom of the reation kettle body is provided with the reation kettle discharge gate, the reation kettle discharge gate is connected with material receiving device, material receiving device's upper end is provided with the interface, the interface passes through pipeline and vacuum pump connection.

Preferably, a feeding port is arranged at the top of the reaction kettle body.

Preferably, the upper end of the reaction kettle body is provided with a first conduction interface, and the lower end of the reaction kettle body is provided with a second conduction interface.

Preferably, the material receiving device is connected to the lower end of the stainless steel support bracket through a material receiving fixing rod.

Preferably, the bottom of the receiving device is obliquely arranged.

Preferably, a discharge hole is formed in the bottom end of the receiving device.

Compared with the prior art, the utility model has the following beneficial effects:

according to the immobilized enzyme reaction device, the preparation and the catalytic reaction of the immobilized enzyme are carried out in the reaction kettle body, meanwhile, the reaction liquid and the immobilized enzyme can be separated, the filtering device consisting of stainless steel and a filter membrane is arranged below the reaction kettle body, the reaction liquid can be easily filtered out through the material receiving device connected with the vacuum pump, the immobilized enzyme is reserved in the reaction kettle body for the next batch reaction, the next batch reaction can be directly fed, the reaction efficiency is greatly improved, the experimental operation is convenient, the pressure of post-treatment is reduced, the reaction liquid and the immobilized enzyme can be effectively separated, and the preparation of the immobilized enzyme can be carried out.

Drawings

FIG. 1 is a schematic diagram of an immobilized enzyme reaction apparatus of the present utility model;

FIG. 2 is a schematic diagram of a reaction kettle body of the utility model connected with a receiving device through a discharge hole of the reaction kettle.

In the figure: 1. a stainless steel support bracket; 2. a kettle body fixing rod; 3. a reaction kettle body; 31. a long rod thermometer; 32. an engine; 33. a stirring device; 34. a filtering device; 35. a feed port; 36. a first conductive interface; 37. a second conductive interface; 4. a discharge hole of the reaction kettle; 5. a receiving device; 51. an interface; 52. a discharge port; 6. a vacuum pump; 7. and a receiving fixing rod.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-2, an immobilized enzyme reaction device comprises a stainless steel support bracket 1, wherein a kettle body fixing rod 2 is arranged at the upper end of the stainless steel support bracket 1, and a reaction kettle body 3 is connected to the kettle body fixing rod 2.

A long rod thermometer 31 is arranged on the reaction kettle body 3.

The long-rod thermometer 31 monitors the temperature during the experiment.

An engine 32 is arranged above the reaction kettle body 3, an output shaft of the engine 32 is connected with a stirring device 33, and the stirring device 33 is positioned in the reaction kettle body 3.

The stirring device 33 uniformly stirs the reaction materials in the reaction kettle body 3 by the power provided by the engine 32.

The inner side of the bottom of the reaction kettle body 3 is provided with a filtering device 34 composed of stainless steel and a filtering membrane.

The top of the reaction kettle body 3 is provided with a feed port 35.

The upper end of the reaction kettle body 3 is provided with a first conduction interface 36, and the lower end of the reaction kettle body 3 is provided with a second conduction interface 37.

The first conduction interface 36 and the second conduction interface 37 are connected to a cold-hot integrated machine to control the temperature in the reaction kettle body 3.

The bottom of the reaction kettle body 3 is provided with a reaction kettle discharge port 4, a bottom valve is arranged on the reaction kettle discharge port 4, the reaction kettle discharge port 4 is connected with a material receiving device 5, and the material receiving device 5 is connected to the lower end of the stainless steel support bracket 1 through a material receiving fixing rod 7.

The bottom of the receiving device 5 is obliquely arranged.

The bottom of the receiving device 5 is designed as an inclined device, so that the material can flow out of the discharge hole 52 conveniently.

The upper end of the receiving device 5 is provided with an interface 51, and the interface 51 is connected with the vacuum pump 6 through a pipeline.

The bottom of the receiving device 5 is provided with a discharge hole 52, and the discharge hole 52 is provided with a bottom valve.

Specifically, enzyme solution and microcrystalline cellulose are added through a feed port 35 to manufacture immobilized enzyme, the immobilized enzyme is filtered by a filtering device 34 below the reaction kettle body 3 through a connecting vacuum pump 6, the immobilized enzyme is recovered, and then reaction liquid is fed through the feed port 35 above the reaction kettle body 3 to perform catalytic reaction; the long-rod thermometer 31 monitors the temperature during the experiment; the first conduction interface 36 and the second conduction interface 37 are connected with a cold-hot integrated machine to control the temperature in the reaction kettle body 3; the stirring device 33 uniformly stirs the reaction materials in the reaction kettle body 3 by the power provided by the engine 32; when the catalytic reaction is finished, a bottom valve of a discharge hole 4 of the reaction kettle is opened, an interface 51 is connected with a vacuum pump 6 to pump and filter reaction liquid in the reaction kettle body 3 into a receiving bottle, and finally, reaction clear liquid directly flows out of the discharge hole of the receiving bottle, and immobilized enzyme in the reaction kettle body 3 can directly carry out the next batch reaction.

In summary, the immobilized enzyme reaction device of the utility model carries out the preparation and catalytic reaction of the immobilized enzyme in the reaction kettle body 3, meanwhile, the reaction liquid and the immobilized enzyme can be separated, the filtering device 34 consisting of stainless steel and a filter membrane is arranged below the reaction kettle body 3, the reaction liquid can be easily filtered out by connecting the material receiving device 5 with the vacuum pump 6, the immobilized enzyme is reserved in the reaction kettle body 3 for the next batch of reaction, the next batch of reaction can be directly fed, the reaction efficiency is greatly improved, the experimental operation is convenient, the pressure of post-treatment is reduced, the reaction liquid and the immobilized enzyme can be effectively separated, and the preparation of the immobilized enzyme can be carried out.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An immobilized enzyme reaction device comprises a stainless steel support bracket (1), and is characterized in that: the reactor comprises a reactor body fixing rod (2) arranged at the upper end of a stainless steel support bracket (1), a reactor body (3) connected to the reactor body fixing rod (2), a long rod thermometer (31) arranged on the reactor body (3), an engine (32) arranged above the reactor body (3), a stirring device (33) connected with an output shaft of the engine (32), the stirring device (33) arranged in the reactor body (3), a filtering device (34) composed of stainless steel and a filter membrane arranged on the inner side of the bottom of the reactor body (3), a reactor discharge outlet (4) arranged at the bottom of the reactor body (3), a material receiving device (5) connected to the reactor discharge outlet (4), and an interface (51) arranged at the upper end of the material receiving device (5) and connected to the interface (51) through a pipeline and a vacuum pump (6).

2. An immobilized enzyme reaction apparatus according to claim 1, wherein: the top of the reaction kettle body (3) is provided with a feed inlet (35).

3. An immobilized enzyme reaction apparatus according to claim 2, wherein: the upper end of the reaction kettle body (3) is provided with a first conduction interface (36), and the lower end of the reaction kettle body (3) is provided with a second conduction interface (37).

4. An immobilized enzyme reaction apparatus according to claim 1, wherein: the receiving device (5) is connected to the lower end of the stainless steel support bracket (1) through a receiving fixing rod (7).

5. An immobilized enzyme reaction apparatus according to claim 1, wherein: the bottom of the receiving device (5) is obliquely arranged.

6. The immobilized enzyme reaction apparatus according to claim 5, wherein: the bottom end of the receiving device (5) is provided with a discharge hole (52).