CN219279888U

CN219279888U - Cell incubator for immune cell expansion

Info

Publication number: CN219279888U
Application number: CN202222428161.3U
Authority: CN
Inventors: 张升; 姜南; 王玉明; 车小红
Original assignee: Beijing Hongxin Stem Cell Biotechnology Co ltd
Current assignee: Beijing Hongxin Stem Cell Biotechnology Co ltd
Priority date: 2022-09-14
Filing date: 2022-09-14
Publication date: 2023-06-30
Anticipated expiration: 2032-09-14

Abstract

The utility model relates to the field of cell culture boxes, in particular to a cell culture box for immune cell expansion, which comprises a cell culture box body, wherein pulleys are arranged at four corners of the bottom end of the cell culture box body, a heating plate is arranged in the cell culture box body, and a control panel is arranged on the front surface of the cell culture box body. According to the utility model, the connecting rod, the servo motor and the angle sensor are arranged, the connecting rod rotates, the angle sensor is coaxially arranged on the servo motor, the back and forth rotation angle is controlled, and the angle is controlled within a range of ten degrees, so that the storage carriage drives the culture vessel to shake for all-weather fixed time, immune cells are cultured in the culture vessel, the culture vessel is horizontally placed on the storage carriage, immune cell agglomeration can be prevented, and the amplification is facilitated.

Description

Cell incubator for immune cell expansion

Technical Field

The utility model relates to the field of cell culture boxes, in particular to a cell culture box for immune cell expansion.

Background

In experiments, cells are cultured in a cell culture incubator, which is a device for culturing cells/tissues in vitro by simulating and forming a growth environment similar to cells/tissues in organisms in the incubator, wherein immune cells refer to a group of cells involved in or related to immune response, and mainly comprise t lymphocytes, b lymphocytes, k lymphocytes and nk lymphocytes. The cell culture box is needed for immune cell expansion.

The utility model discloses a voice recognition's cell culture case is disclosed to the authority bulletin number CN212955177U, including the cell culture case body, the top of cell culture case body is provided with the control box, the top at the incubator body is fixed to the control box, articulated have the chamber door on the incubator body, be fixed with the display screen on the chamber door, one side of display screen is fixed with voice recognition ware, be fixed with intelligent lock on the chamber door, the top of control box is fixed in face identification camera, the inner chamber top of cell culture case body is fixed with the sterilamp, the inner chamber bottom of cell culture case body is fixed with the fixed plate, fixed mounting has the shower nozzle of a plurality of circumference range on the fixed plate, a plurality of the bottom of shower nozzle is fixed with the circular pipe. The utility model can realize voice adjustment of various parameters, does not need manual adjustment, can carry out face recognition, improves the safety, can double sterilize the inner cavity of the incubator body, and can maintain a good sterile environment.

However, in the existing immune cells in the process of culturing, the existing immune cells are easy to grow in a holding manner, so that cells in the middle of the groups cannot be sufficiently nourished, cell death and expansion reduction are caused, the doors of other cell culture boxes are fully shielded, the internal working condition of the box is not visible, the simple viewing window is not clear enough, bacterial impurities can be caused by directly opening the doors for viewing, and immune cell expansion is interrupted, so that a cell culture box for immune cell expansion needs to be designed to solve the problems.

Disclosure of Invention

Aiming at the problems that the prior cell incubator has a fully-shielded door, the working condition inside the incubator is not visible, the observation of a simple window is not clear enough, and the direct opening of the door can lead to the entry of microorganisms such as bacteria and the like, thus leading to the pollution of immune cells and the like, the cell incubator for amplifying immune cells is provided.

The utility model provides a cell culture box for immune cell expansion, which comprises a cell culture box body, wherein pulleys are arranged at four corners of the bottom end of the cell culture box body, a heating plate is arranged in the cell culture box body, a control panel is arranged on the front surface of the cell culture box body, a box door is hinged to one side of the cell culture box body, a servo motor is arranged in the cell culture box body, a connecting rod is arranged on one side of an output shaft of the servo motor, a storage carriage is arranged at the top end of the connecting rod, and a clamping mechanism is uniformly arranged at the top end of the storage carriage; the inside of the box door is provided with a concave cylinder, the inner side of the concave cylinder is embedded with anti-fog toughened sealing glass, the other side of the concave cylinder is provided with a supporting rod, and the inside of the supporting rod is inserted with an observation cylinder in a sliding way.

Preferably, the side of the observation cylinder, which is close to the anti-fog toughened sealing glass, is inclined downwards by twenty-five degrees.

Preferably, a lens on one side of the observation tube, which is close to the anti-fog toughened sealing glass, is a telescope lens, and a handle is arranged on one side of the box door.

Preferably, a heat-insulating rock wool is arranged between the box door and the cell culture box body in a shell interlayer.

Preferably, the top end of the cell culture box body is transversely provided with ultraviolet light tubes, and the number of the ultraviolet light tubes is two.

Preferably, the heat insulation sleeves are symmetrically arranged on one side of the storage planker.

Preferably, the clamping mechanism comprises a rectangular box, a rectangular groove is formed in the top end of the rectangular box, a telescopic spring is arranged in the rectangular box, a sliding rod is arranged on one side of the telescopic spring and is in sliding connection with the rectangular box, a movable rod is arranged on the top end of one side of the rectangular box in a sleeved mode, a movable semi-ring is arranged on one side of the sliding rod, and a fixed semi-ring is arranged on the top end of the storage carriage.

Preferably, a culture vessel is clamped between the movable half ring and the fixed half ring, and a fastening pad is arranged on one side of the movable half ring and one side of the fixed half ring, which are close to each other.

Compared with the prior art, the utility model has the beneficial effects that:

1. through being provided with connecting rod, servo motor and angle sensor, the connecting rod rotates, and servo motor coaxial mounting has angle sensor, and control back and forth pivoted angle, control is in ten degrees within range for deposit the planker drive can all-weather fixed time shake the culture vessel, immune cells fosters in the culture vessel, and the culture vessel keeps flat and stores on the planker, can prevent immune cells agglomeration, more does benefit to the expansion.

2. Through being provided with observation section of thick bamboo, bracing piece, antifog toughened sealing glass and indent section of thick bamboo, can observe the inside approximate condition of cell culture box through antifog toughened sealing glass, the staff of being convenient for knows the internal device operating condition, through sliding the observation section of thick bamboo for one side of observation section of thick bamboo supports antifog toughened sealing glass, goes to observe the amplification condition in the immune cell storage dish through one side of observation section of thick bamboo, and the staff of being convenient for directly observes in the outside, avoids influencing cell reproduction; the downward inclination of one side of the observation cylinder is convenient for a worker to put the observation vessel from top to bottom in view angle; the telescope lens can clearly see the vessel at a distance.

3. Through being provided with the block mechanism, the telescopic spring extrudees the slide bar for movable semi-ring extrusion culture vessel, with fixed semi-ring cooperation block culture vessel, stir the movable rod, make the movable rod extrusion telescopic spring, draw the inside of rectangle box into the slide bar, the movable semi-ring of being convenient for no longer extrudes the culture vessel, makes the culture vessel be convenient for take out.

Drawings

FIG. 1 is a schematic perspective view of the present utility model;

FIG. 2 is a schematic view showing a door open display perspective structure of the present utility model;

FIG. 3 is a schematic perspective view of a storage carriage according to the present utility model;

fig. 4 is a schematic perspective view of the engaging mechanism of the present utility model.

In the figure: 110. a cell culture box; 120. a control panel; 130. a door; 131. a handle; 132. an observation tube; 133. a support rod; 134. anti-fog toughened sealing glass; 135. a concave cylinder; 140. an ultraviolet lamp tube; 150. a heating plate; 160. storing the carriage; 161. a servo motor; 162. a connecting rod; 163. a heat insulating sleeve; 200. a clamping mechanism; 210. a rectangular box; 211. rectangular grooves; 212. a telescopic spring; 213. a moving rod; 214. a slide bar; 215. a culture vessel; 216. a stationary half ring; 217. a movable semi-ring.

Detailed Description

Example 1

Referring to fig. 1-2, an embodiment of the present utility model is provided: the utility model provides a cell culture box for immune cell expansion, including cell culture box 110, four corners of cell culture box 110 bottom are provided with the pulley, the inside of cell culture box 110 is provided with hot plate 150, the front of cell culture box 110 is provided with control panel 120, chamber door 130 is installed to one side hinge of cell culture box 110, the inside of cell culture box 110 is provided with servo motor 161, one side of servo motor 161's output shaft is provided with connecting rod 162, the top of connecting rod 162 is provided with deposits planker 160, deposit planker 160's top even be provided with block mechanism 200; the inside of the box door 130 is provided with a concave cylinder 135, an anti-fog toughened sealing glass 134 is embedded in the inner side of the concave cylinder 135, the other side of the concave cylinder 135 is provided with a supporting rod 133, and an observation cylinder 132 is inserted in the supporting rod 133 in a sliding manner; the servo motor 161 is started to drive the connecting rod 162 to rotate, the angle sensor is coaxially arranged on the servo motor 161, the back and forth rotation angle is controlled, and the angle is controlled within a range of ten degrees, so that the storage carriage 160 drives the culture vessel 215 to shake in all-weather fixed time, immune cells are cultured in the culture vessel 215, the culture vessel 215 is horizontally placed on the storage carriage 160, immune cell agglomeration can be prevented, and the amplification is facilitated.

Can observe the inside approximate condition of cell culture box 110 through antifog toughened sealing glass 134, the staff of being convenient for knows the internal device operating condition, through sliding observation tube 132 for one side of observation tube 132 supports antifog toughened sealing glass 134, goes to observe the expansion condition in the immune cell storage dish through one side of observation tube 132, and the staff of being convenient for directly observes in the outside, avoids influencing cell reproduction.

Further, the side of the observation tube 132 near the anti-fog tempered sealing glass 134 is inclined downward by twenty-five degrees; because the vessel is difficult to see in a horizontal state, the vessel is convenient to observe by a worker from top to bottom in a visual angle.

Further, a lens on one side of the observation tube 132, which is close to the anti-fog toughened sealing glass 134, is a telescope lens, and a handle 131 is arranged on one side of the box door 130; the telescope lens can well see the vessel at a distance clearly, and observe the propagation condition of cells.

Example two

In the cell incubator for immune cell expansion according to the present utility model, compared with the first embodiment, referring to fig. 1, a heat-insulating rock wool is arranged between a case body interlayer of the cell incubator 110 and a case door 130; the temperature of the equipment is protected, and the loss of hot gas is avoided.

Further, the top end of the cell culture box 110 is transversely provided with two ultraviolet lamps 140, and the number of the ultraviolet lamps 140 is two; the ultraviolet lamp 140 can be used after sterilizing the inside of the cell culture case 110, so that immune cells can be amplified in a pollution-free and impurity-free environment.

Example III

In the cell incubator for immune cell expansion according to the present utility model, compared with the first embodiment, referring to fig. 3-4, a heat insulation sleeve 163 is symmetrically disposed on one side of the storage carriage 160; the insulating sleeve 163 can be convenient for the staff take out the cultivation household utensils 215 of depositing on the planker 160, deposits the planker 160 for heat conduction material, and insulating sleeve 163 thermal-insulated protection staff avoids scalding.

Further, the locking mechanism 200 includes a rectangular box 210, a rectangular slot 211 is formed at the top end of the rectangular box 210, a telescopic spring 212 is arranged in the rectangular box 210, a sliding rod 214 is arranged on one side of the telescopic spring 212, the sliding rod 214 is slidably connected with the rectangular box 210, a moving rod 213 is arranged at the top end of one side of the rectangular box 210 in a sleeved mode, a movable half ring 217 is arranged on one side of the sliding rod 214, and a fixed half ring 216 is arranged at the top end of the storage carriage 160; the telescopic spring 212 pushes the sliding rod 214, so that the movable semi-ring 217 extrudes the culture vessel 215, the fixed semi-ring 216 is matched with the fixed semi-ring 216 to clamp the culture vessel 215, the moving rod 213 is shifted, the moving rod 213 extrudes the telescopic spring 212, the sliding rod 214 is pulled into the rectangular box 210, the movable semi-ring 217 is convenient to not extrude the culture vessel 215 any more, and the culture vessel 215 is convenient to take out.

Further, a culture vessel 215 is clamped between the movable half ring 217 and the fixed half ring 216, and a fastening pad is arranged on one side of the movable half ring 217 and the fixed half ring 216, which is close to each other; the fastening pad increases the stability of the grip so that wear of the outer shell of the culture vessel 215 is avoided.

The working principle of the utility model is as follows: the servo motor 161 starts the connecting rod 162 to rotate, the angle sensor is coaxially arranged on the servo motor 161, the back and forth rotation angle is controlled, the angle is controlled within a range of ten degrees, the storage carriage 160 drives the shake culture vessel 215 which can be used for fixing time in all weather, immune cells are cultured in the culture vessel 215, the culture vessel 215 is horizontally placed on the storage carriage 160, the immune cells can be prevented from agglomerating, the expansion is facilitated, the situation that the interior of the cell culture box 110 is approximate can be observed through the anti-fog toughened sealing glass 134, workers can know the working state of internal devices conveniently, one side of the observation cylinder 132 props against the anti-fog toughened sealing glass 134 through sliding the observation cylinder 132, the expansion situation in the immune cell storage vessel is observed through one side of the observation cylinder 132, the workers can observe the immune cells directly outside conveniently, and the influence on cell propagation is avoided; one side of the observation tube 132 near the anti-fog toughened sealing glass 134 is inclined downwards by twenty-five degrees; the observation vessel is conveniently placed from top to bottom at the visual angle of the staff; the telescope lens can clearly see the vessel at a distance.

The telescopic spring 212 extrudes the sliding rod 214, so that the movable semi-ring 217 extrudes the culture vessel 215, and the fixed semi-ring 216 is matched with the clamped culture vessel 215, the moving rod 213 is shifted, so that the moving rod 213 extrudes the telescopic spring 212, the sliding rod 214 is pulled into the rectangular box 210, the movable semi-ring 217 is convenient to not extrude the culture vessel 215 any more, and the culture vessel 215 is convenient to take out.

The embodiments of the present utility model have been described in detail with reference to the drawings, but the present utility model is not limited thereto, and various changes can be made within the knowledge of those skilled in the art without departing from the spirit of the present utility model.

Claims

1. The cell culture box for immune cell expansion is characterized by comprising a cell culture box body (110), pulleys are arranged at four corners of the bottom end of the cell culture box body (110), a heating plate (150) is arranged in the cell culture box body (110), a control panel (120) is arranged on the front surface of the cell culture box body (110), a box door (130) is hinged to one side of the cell culture box body (110), a servo motor (161) is arranged in the cell culture box body (110), a connecting rod (162) is arranged on one side of an output shaft of the servo motor (161), a storage planker (160) is arranged at the top end of the connecting rod (162), and a clamping mechanism (200) is uniformly arranged at the top end of the storage planker (160);

the inside of chamber door (130) is provided with indent section of thick bamboo (135), and the inboard embedding of indent section of thick bamboo (135) is provided with antifog toughened sealing glass (134), and the opposite side of indent section of thick bamboo (135) is provided with bracing piece (133), and the inside slip of bracing piece (133) has inserted observation section of thick bamboo (132).

2. A cell culture incubator for immune cell expansion according to claim 1, wherein the side of the viewing cylinder (132) adjacent to the anti-fog tempered sealing glass (134) is inclined downwards by twenty-five degrees.

3. The cell culture incubator for immune cell expansion according to claim 1, wherein a lens of a side of the observation tube (132) close to the anti-fog tempered sealing glass (134) is a telescope lens, and a handle (131) is arranged on a side of the door (130).

4. A cell culture incubator for immune cell expansion according to claim 1, characterized in that a shell interlayer between the door (130) and the cell culture incubator body (110) is provided with insulating rock wool.

5. The cell culture incubator for immune cell expansion according to claim 1, wherein ultraviolet lamp tubes (140) are transversely arranged at the top end of the cell culture incubator body (110), and the number of the ultraviolet lamp tubes (140) is two.

6. The cell culture incubator according to claim 1, wherein the heat insulation sleeve (163) is symmetrically arranged at one side of the carriage (160).

7. The cell incubator for immune cell expansion according to claim 1, wherein the clamping mechanism (200) comprises a rectangular box (210), a rectangular groove (211) is formed in the top end of the rectangular box (210), a telescopic spring (212) is arranged in the rectangular box (210), a sliding rod (214) is arranged on one side of the telescopic spring (212), the sliding rod (214) is in sliding connection with the rectangular box (210), a movable rod (213) is arranged on the top end of one side of the rectangular box (210) in a sleeved mode, a movable half ring (217) is arranged on one side of the sliding rod (214), and a fixed half ring (216) is arranged on the top end of the storage carriage (160).

8. Cell incubator for immune cell expansion according to claim 7, characterized in that a culture vessel (215) is clamped between the movable half ring (217) and the stationary half ring (216), and a fastening pad is arranged on the side of the movable half ring (217) and the stationary half ring (216) close to each other.