CN219266168U

CN219266168U - Agarose gel electrophoresis tank integrating glue making and electrophoresis

Info

Publication number: CN219266168U
Application number: CN202220176626.8U
Authority: CN
Inventors: 邓颖
Original assignee: Jianghan University
Current assignee: Jianghan University
Priority date: 2022-01-21
Filing date: 2022-01-21
Publication date: 2023-06-27
Anticipated expiration: 2032-01-21

Abstract

The utility model discloses a gel-making and electrophoresis integrated agarose gel electrophoresis tank, which comprises an electrophoresis tank, wherein two opposite sides of the electrophoresis tank are respectively provided with an electrode anode and an electrode cathode, and is characterized by also comprising a rectangular gel tank arranged in the middle of an inner cavity of the electrophoresis tank, wherein the edge of the gel tank is 2mm high, the thickness is not less than 3mm, and the area surrounded by the inner periphery of the gel tank is 10000-10304 mm ² The edges of the gel tank are spaced apart from the interior cavity of the electrophoresis tank, and the edges of the electrophoresis tank are at least 3mm higher than the edges of the gel tank. Solves the defects and production of colloid and sample holes in the process of preparing the colloidWaste of raw experimental materials.

Description

Agarose gel electrophoresis tank integrating glue making and electrophoresis

Technical Field

The utility model relates to the technical field of gel electrophoresis systems, in particular to an agarose gel electrophoresis tank integrating gel preparation and electrophoresis.

Background

Gel electrophoresis experiments are well known in which the gel is placed in an electrophoresis tank filled with buffer, with the top of the gel just immersed in the buffer. The starting materials for the gel matrix have structural and porous characteristics suitable for containing and separating proteins of interest of specific molecular weight, size, composition. The gel also has loading wells for placement of the target sample for testing. The electrophoresis tank has a cathode and an anode at two ends of gel, so that an electric field formed after current is conducted acts on the sample in the sample loading hole. The electric field causes the charged molecules to separate in the gel such that negatively charged molecules move through the gel to the anode at different rates in the form of a band, while positively charged molecules move through the gel to the cathode at different rates in the form of a band.

At present, in a DNA electrophoresis apparatus commonly used in a laboratory, the main implementation scheme is that the gel preparation and the electrophoresis are respectively independent and matched with each other. CN204666555U discloses a glue making mould, when in use, melted agar is poured into an area formed by a bottom plate and a glue making groove together, gel is transferred from the glue making groove to an electrophoresis groove after glue making is completed, and gel is poured into a double-layer mould in the process of pouring melted agar sometimes, so that a certain agarose waste is caused.

Thus, there is a continuing need for a simple, easy to use, improved electrophoresis apparatus that requires minimal processing and handling steps, has structural and performance stability, is time-saving, is low cost, and is partially a disposable consumable item that avoids the time spent cleaning and maintenance of the apparatus.

Disclosure of Invention

The utility model aims to solve the problems in the prior art and provide an agarose gel electrophoresis tank integrating glue making and electrophoresis.

The above object of the present utility model is achieved by the following technical solutions: the utility model provides a system is glued and electrophoresis integral type agarose gel electrophoresis tank, includes the electrophoresis tank, and two opposite sides of electrophoresis tank set up electrode positive pole and electrode negative pole respectively, still including setting up the rectangle gel groove in the middle of the inner chamber of electrophoresis tank, the edge height of gel groove is 2mm, and the degree is not less than 3mm, and the area that the inner periphery of gel groove encircled is 10000 ~ 10304mm ² The edge of the gel tank is spaced from the inner cavity of the electrophoresis tank, and the edge of the electrophoresis tank is at least 3mm higher than the edge of the gel tank.

The inner peripheral dimensions of the gel tank were 112mm×92mm, and the thickness of the edge of the gel tank was 4mm.

The electrophoresis tank is a set of electrophoresis box made of hard plastic, glass or glass material which is integrally molded, the upper surface of the electrophoresis box is opened, and the electrophoresis box is formed by a groove made of ultraviolet penetrable material, so that gel in the gel tank can be directly observed and shot when the electrophoresis tank is placed on an ultraviolet lamp box.

A gel comb is arranged between two opposite sides between the positive electrode and the negative electrode of the electrophoresis tank electrode, two ends of the gel comb are provided with edges of the U-shaped clamping grooves clamped with the electrophoresis tank, a series of comb teeth extend downwards from the lower surface of the gel comb and extend into the gel to form a series of sample holes for filling target samples, and any number of comb teeth can be arranged according to the number of the target samples to form corresponding number of lanes.

The gel solution adopts agarose gel or any other gel suitable for electrophoresis, the gel solution and the side wall of the gel tank have smaller surface tension, after the gel tank is fully filled with the gel solution, the gel solution expands to the periphery and the upper side along the edge of the gel tank, and the gel with the edge size not more than 3mm of the inner wall of the gel tank and the height not more than 3mm of the upper end surface of the gel tank is formed on the upper end surface of the gel tank, so that after the gel solution is poured, the gel can be ensured not to overflow into the space between the inner cavity of the electrophoresis tank and the edge of the gel tank as long as the liquid level of the gel solution is kept not more than 3mm of the upper end surface of the gel tank or the edge of the gel solution is kept not more than 3mm of the edge of the inner wall of the gel tank. Thus, it was found from the calculation that when the edge of the gel solution comes into contact with the outside of the edge of the gel tank having a thickness of 3mm, the volume of the gel in the gel tank was = (112×92×1+120×100×3) =46 ml. The gel volume is close to the maximum gel volume required by most gel electrophoresis experiments, and the requirements of most experiments are met.

If the inner peripheral dimension of the gel tank is 100mm×100mm, the gel volume is 40-53 ml when the gel solution is 3-4mm higher than the gel tank.

The application method of the device comprises the following steps: filling a gel tank with a hot gel solution, setting the liquid level of the gel solution to be 1-3 mm higher than the gel tank, erecting a gel comb on the gel tank, pouring an electrophoresis buffer solution into the electrophoresis tank after the gel solution on the gel tank is solidified, taking out the gel comb, placing a sample in a sample hole, switching on the anode of an electrode and the cathode of the electrode, and allowing the current to pass through the gel immersed in the buffer solution as long as the current exists in the buffer solution, so that the sample staying in the sample hole is separated in the gel under the action of an electric field, and the molecules with negative charges move to the anode in a strip form through the gel at different speeds, and the molecules with positive charges move to the cathode in a strip form through the gel at different speeds. Some electrophoresis devices in conventional electrophoresis experiments are designed to occupy the full width of the cell, but in operation the electrophoresis liquid will be higher than the upper surface of the gel, so that the gel is completely immersed, and thus the gel need not be completely separated from the two chambers containing the electrophoresis liquid.

The device is used for preparing the gel, and gel is not required to be transferred again after being prepared, so that the defect of the gel caused by the gel adhering to the inner cavity of the electrophoresis tank is avoided; in addition, due to the integrated structure of the gel groove and the electrophoresis tank, before the gel comb is pulled out, the buffer solution is poured into the electrophoresis tank, and when the comb is pulled out, the buffer solution can permeate into gaps between the comb and gel, so that the adhesion between the comb and the gel is avoided, the separation of the comb and the gel is facilitated, and the defect of sample holes on the gel is avoided when the comb is pulled out. In the prior art, the gel tank and the electrophoresis tank are separated from each other independently, and the buffer solution is poured into the gel tank to waste experimental materials. Therefore, the device of the utility model also solves the problems of incomplete colloid and sample hole and waste of experimental materials in the process of preparing the colloid.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

Detailed Description

The present utility model will be further described in detail below in conjunction with the following examples, for the purpose of facilitating understanding and practicing the present utility model by those of ordinary skill in the art, it being understood that the examples described herein are for the purpose of illustration and explanation only and are not intended to limit the utility model.

Example 1:

the utility model provides a system is glued and electrophoresis integral type agarose gel electrophoresis tank, includes electrophoresis tank 1, electrophoresis tank 1's two opposite sides set up electrode positive pole 11 and electrode negative pole 12 respectively, still including setting up the rectangle gel tank 2 in the middle of electrophoresis tank 1's the inner chamber, the edge height 2mm of gel tank 2, thickness is not less than 3mm, the inner peripheral dimension of gel tank 2 is 116mm x 96mm, the edge of gel tank 2 separates with electrophoresis tank 1's inner chamber interval, electrophoresis tank 1 edge is higher than the edge of gel tank 2 at least 3mm.

The electrophoresis tank 1 is a set of electrophoresis box made of hard plastic or organic glass material and integrally molded, the upper surface of the electrophoresis box is opened, and the electrophoresis box is formed by a groove made of ultraviolet penetrable material, so that gel in the gel tank 2 can be directly observed and shot when the electrophoresis tank is placed on an ultraviolet lamp box.

Gel combs 3 are arranged between two opposite sides between the electrophoresis tank electrode anode 11 and the electrode cathode 12, two ends of each gel comb 3 are provided with U-shaped clamping grooves which are clamped with the edges of the electrophoresis tank 1, a series of comb teeth 31 extend downwards from the lower surface of each gel comb 3 and extend into the gel to form a series of sample holes for filling target samples, and any number of comb teeth can be arranged according to the number of the target samples to form corresponding number of lanes.

Preferably, the thickness of the edge of the gel tank 2 is 4mm.

The gel solution generally adopts agarose gel, when the gel solution is fully filled in the gel groove, the liquid gel can form a glue layer with the thickness of at least 4-5mm on the upper end surface of the gel groove due to the action of surface tension, which is higher than the glue groove by about 3-4mm, without overflowing the glue groove, and after the gel is solidified, the agarose gel with the thickness of at least 4-5mm can be formed. The upkeep volume in conventional DNA detection electrophoresis is 5-15ul, and the electrophoresis loading volume in gel recovery is generally 50ul. The volume of the colloid with the height of 4mm is = (112×92×1+120×100×3) =0.046ml=46 ml, and the requirement of the sample loading quantity can be met by matching with the colloid comb teeth with the conventional size. The length and width of a comb single tooth for general detection are 2-3mm, the length and width of the comb during recycling and glue making are 10mm respectively and 2-3mm, and the size of a sample feeding groove finally formed by matching with the height of the glue can meet the experimental requirements.

The specific embodiments described herein are offered by way of example only to illustrate the spirit of the utility model. Those skilled in the art may make various modifications or additions to the described embodiments or substitutions thereof without departing from the spirit of the utility model or exceeding the scope of the utility model as defined in the accompanying claims.

Claims

1. The utility model provides a make and electrophoresis integral type agarose gel electrophoresis tank, includes the electrophoresis tank, and two opposite sides of electrophoresis tank set up electrode positive pole and electrode negative pole respectively, its characterized in that still includes the rectangle gel groove that sets up in the middle of the inner chamber of electrophoresis tank, the edge height of gel groove is 2mm, and thickness is not less than 3mm, and the area that the inner periphery of gel groove encircled is 10000 ~ 10304mm ² The edges of the gel tank are spaced apart from the interior cavity of the electrophoresis tank, and the edges of the electrophoresis tank are at least 3mm higher than the edges of the gel tank.