CN219251720U - Dynamic axial compression chromatographic column without hydraulic cylinder - Google Patents

Abstract

The utility model discloses a dynamic axial compression chromatographic column without a hydraulic cylinder, which comprises a piston body, a piston rod, a sealing gasket arranged between the piston body and the piston rod and a connecting assembly used for movably connecting the piston rod and the piston body, wherein a first cambered surface part is arranged on the piston body, a second cambered surface part matched with the first cambered surface part is arranged on the piston rod, one side of the sealing gasket is clung to the first cambered surface part, and the other side of the sealing gasket is clung to the second cambered surface part. The piston rod is movably connected with the piston body through the connecting component, and is matched with the first cambered surface part through the second cambered surface part, so that the piston rod can be automatically compensated and corrected under the condition of angular displacement, and the requirements on machining precision and mounting precision can be reduced, and the cost is reduced; the sealing gasket between the piston rod and the piston body is respectively clung to the piston rod and the piston body, so that high-pressure sealing connection can be realized, the pressure of the mobile phase in the chromatographic column can not be reduced due to liquid leakage, and the pressure of a bed below the piston body is further ensured.

Description

Dynamic axial compression chromatographic column without hydraulic cylinder

Technical Field

The utility model relates to a chromatographic column, in particular to a dynamic axial compression chromatographic column without a hydraulic cylinder.

Background

The dynamic axial compression chromatographic column without the hydraulic cylinder mainly comprises a chromatographic column tube, a piston body, an upper column cover and a lower column cover, wherein the piston body is provided with a piston rod and is arranged in the column tube, the column tube is divided into an upper cavity and a lower cavity, and a mobile phase is pumped into the upper cavity through an infusion pump so as to drive the piston body to move downwards. Because the stroke of the piston body is longer, and meanwhile, the processing precision, the installation error and other external factors which cannot be overcome are caused, the coaxiality between the five parts cannot be ensured (mainly, the piston rod generates angular displacement relative to the piston body), particularly, the pressure of a mobile phase reaches 10MPa under the working state of the chromatographic column, the sealing between the piston rod and the piston body is more difficult to realize, and the coaxiality and the sealing technical difficulty cannot be solved, so that no hydraulic cylinder column technology is practically applied at present, and the equipment in the market only can adopt a hydraulic cylinder mode and is forced to accept the pain points such as hydraulic oil pollution, high operating environment requirement and the like brought by the hydraulic cylinder.

In addition, after the homogenate is injected into the column tube, the piston body of the chromatographic column is driven by the hydraulic cylinder to move downwards to press out the mobile phase in the homogenate, so that the filler can be uniformly remained in the column tube and compacted. Currently, there are two main modes of injection of homogenates: 1. raising the piston above the column tube, pumping the homogenate into the chromatographic column by an infusion pump, and then depressing the piston; 2. the piston body is placed in the chromatographic column, and the position below the piston body of the side wall of the chromatographic column is provided with holes, and the homogenate is pumped into the chromatographic column through the holes. Both of these two approaches have major drawbacks: in the first mode, impurities are easily brought into the homogenate by pumping, and in the second mode, dead volume or residues among holes are easy to deteriorate and mold, potential health hidden dangers are obvious, and the potential health hidden dangers do not meet corresponding regulation requirements; the filler in the homogenate is pumped into the column casing and then begins to subside immediately due to the action of gravity, the homogenate is continuously poured in, the impact force generated further accelerates the settling filler, so that the compactness of the packed filler column bed is uneven or sugar cores appear after final filling, the separation and purification effects of equipment are directly influenced, the production performance of the equipment is greatly reduced naturally, and the negative acting force influence is larger along with the increase of the diameter of the chromatographic column, which is another pain point of the equipment.

Disclosure of Invention

The utility model aims to overcome the defects of the prior art, and provides the hydraulic cylinder-free dynamic axial compression chromatographic column which has the advantages of simple structure, low processing cost and low installation precision requirement and is beneficial to ensuring the sealing connection under a high pressure state.

In order to solve the technical problems, the utility model adopts the following technical scheme:

the utility model provides a no pneumatic cylinder dynamic axial compression chromatographic column, includes piston body and piston rod, still including locating the sealed pad and be used for with piston rod and piston body swing joint's coupling assembling between piston body and the piston rod, be equipped with first cambered surface portion on the piston body, be equipped with on the piston rod with first cambered surface portion complex second cambered surface portion, sealed pad one side with first cambered surface portion hugs closely, sealed pad opposite side with second cambered surface portion hugs closely.

As a further improvement of the above technical scheme: the connecting assembly comprises a connecting flange and a plurality of fasteners which are distributed along the circumferential direction of the connecting flange at intervals, a radial convex part is arranged on the piston rod, the connecting flange is sleeved on the piston rod and is abutted to the end face of the radial convex part, a gap is reserved between the inner wall of the connecting flange and the outer wall of the piston rod, and the fasteners are connected with the piston body.

As a further improvement of the above technical scheme: the connecting flange is provided with an axial convex part, and a gap is reserved between the inner wall of the axial convex part and the outer wall of the radial convex part.

As a further improvement of the above technical scheme: the piston rod is a hollow rod and is internally provided with a sample tube in a penetrating way.

As a further improvement of the above technical scheme: and a transition fillet is arranged between the inner wall of the piston rod and the second cambered surface part.

As a further improvement of the above technical scheme: the piston body is provided with a positioning part, the positioning part extends into the piston rod, the first arc surface part is positioned on the periphery of the positioning part, and the sealing gasket is sleeved on the positioning part.

As a further improvement of the above technical scheme: the dynamic axial compression chromatographic column without the hydraulic cylinder further comprises a column tube, an upper cover arranged at the upper end of the column tube, a lower cover arranged at the lower end of the column tube and a slurry feeding valve arranged on the lower cover.

As a further improvement of the above technical scheme: the slurry feeding valve comprises a valve seat connected with a lower cover, a feeding pipe arranged in the valve seat, a valve core for plugging the feeding pipe and an opening and closing assembly for driving the valve core to reciprocate in the feeding pipe, wherein the upper end and the lower end of the outer wall of the feeding pipe are in sealing fit with the valve seat, a feeding gap is formed between the middle section and the valve seat, a feeding interface communicated with the feeding gap is arranged on the valve seat, a feeding port communicated with the feeding gap is arranged on the side wall of the feeding pipe, and a discharging port communicated with the column pipe is arranged at the top of the feeding port.

As a further improvement of the above technical scheme: the valve core is characterized in that the opening and closing assembly comprises a pneumatic motor connected with the valve seat and a connecting nut connected with the pneumatic motor, the upper end of the valve core is positioned in the feeding pipe, and the lower end of the valve core is provided with external threads and is connected with the connecting nut.

As a further improvement of the above technical scheme: the upper end of the valve seat extends into the lower cover, a liquid outlet gap is formed between the outer wall of the valve seat and the lower cover, and a liquid outlet port communicated with the liquid outlet gap is formed in the valve seat.

Compared with the prior art, the utility model has the advantages that: according to the hydraulic cylinder-free dynamic axial compression chromatographic column disclosed by the utility model, the piston rod is movably connected with the piston body through the connecting component, and the piston rod is matched with the first cambered surface part through the second cambered surface part, so that the hydraulic cylinder-free dynamic axial compression chromatographic column can be automatically compensated and corrected under the condition that the piston rod is subjected to angular displacement, and therefore, the requirements on machining precision and mounting precision can be reduced, and the cost is reduced; the sealing gasket between the piston rod and the piston body is respectively clung to the piston rod and the piston body, so that high-pressure sealing connection can be realized, the pressure of the mobile phase in the chromatographic column can not be reduced due to liquid leakage, and the pressure of a bed below the piston body is further ensured.

Drawings

FIG. 1 is a schematic diagram of the structure of a hydraulic cylinder-free dynamic axial compression chromatographic column of the utility model.

Fig. 2 is an enlarged view of a connection portion of the piston rod and the piston body in the present utility model.

FIG. 3 is a schematic first cross-sectional view of the present utility model showing the slurry feed valve closed.

FIG. 4 is a schematic view of a second cross-sectional configuration of the slurry feed valve of the present utility model in a closed state.

FIG. 5 is a schematic first sectional view of the slurry feed valve of the present utility model in an open state.

FIG. 6 is a schematic view of a second cross-sectional structure of the utility model showing the slurry feed valve in an open state.

The reference numerals in the drawings denote: 1. a piston body; 11. a first arc surface portion; 12. a positioning part; 2. a piston rod; 21. a second arc surface portion; 22. a radial protrusion; 23. a transition fillet; 3. a sealing gasket; 4. a connection assembly; 41. a connecting flange; 42. a fastener; 43. an axial protrusion; 5. a sample tube; 6. a column tube; 61. an upper cover; 62. a lower cover; 7. a slurry feed valve; 71. a valve seat; 72. a feed pipe; 721. a feed inlet; 722. a discharge port; 73. a valve core; 74. an opening and closing assembly; 741. a pneumatic motor; 742. a coupling nut; 75. a feed gap; 76. a feed port; 77. a liquid outlet gap; 78. and a liquid outlet interface.

Detailed Description

As used in this section and in the claims, the terms "a," "an," "the," and/or "the" are not specific to a singular, but may include a plurality, unless the context clearly dictates otherwise. The use of the terms "first," "second," and the like in this section does not denote any order, quantity, or importance, but rather are used to distinguish one element from another. Likewise, the word "comprising" or "comprises", and the like, means that elements or items preceding the word are included in the element or item listed after the word and equivalents thereof, but does not exclude other elements or items. The terms "connected" or "connected," and the like, are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.

The utility model is described in further detail below with reference to the drawings and specific examples of the specification.

Fig. 1 to 6 show an embodiment of a hydraulic cylinder-free dynamic axial compression chromatographic column of the utility model, which comprises a piston body 1, a piston rod 2, a sealing gasket 3 arranged between the piston body 1 and the piston rod 2, and a connecting component 4 for movably connecting the piston rod 2 with the piston body 1, wherein a first arc surface part 11 is arranged on the piston body 1, a second arc surface part 21 matched with the first arc surface part 11 is arranged on the piston rod 2, one side of the sealing gasket 3 is tightly attached to the first arc surface part 11, and the other side of the sealing gasket 3 is tightly attached to the second arc surface part 21. Preferably, the sealing gasket 3 is a Polytetrafluoroethylene (PTFE) sealing gasket, has good chemical corrosion resistance and long service life, has self-lubricating property, and is convenient for the piston rod 2 to rotate relative to the piston body 1.

According to the hydraulic cylinder-free dynamic axial compression chromatographic column, the piston rod 2 is movably connected with the piston body 1 through the connecting component 4, and is matched with the first arc surface part 11 through the second arc surface part 21, so that automatic compensation and correction can be realized under the condition that the piston rod 2 is subjected to angular displacement, and the requirements on machining precision and mounting precision can be reduced, and the cost is reduced; the sealing gasket 3 between the piston rod 2 and the piston body 1 is respectively clung to the piston rod and the piston body, so that high-pressure sealing connection can be realized, the pressure of the mobile phase in the chromatographic column cannot be reduced due to liquid leakage, and the pressure of a column bed below the piston body 1 is further ensured.

Further, in this embodiment, the connecting assembly 4 includes a connecting flange 41 and a plurality of fasteners 42 (preferably fastening bolts, screws, etc.) distributed at intervals along the circumferential direction of the connecting flange 41, the piston rod 2 is provided with a radial protrusion 22, the connecting flange 41 is sleeved on the piston rod 2 and abuts against the end face of the radial protrusion 22, a gap is formed between the inner wall of the connecting flange 41 and the outer wall of the piston rod 2, and the fasteners 42 are connected with the piston body 1. Referring specifically to fig. 2, the fastening members 42 distributed at intervals in the circumferential direction of the connecting flange 41 are connected with the piston body 1, so that the connecting flange 41 can press the piston rod 2 downwards through the radial convex parts 22, thereby pressing the sealing gasket 3, realizing high-pressure sealing connection, and meanwhile, a gap is reserved between the inner wall of the connecting flange 41 and the outer wall of the piston rod 2, so that the piston rod 2 can rotate randomly along an arc surface within a certain range without being blocked, and the structure is simple and reliable.

Further, in the present embodiment, the connecting flange 41 is provided with an axial protrusion 43, and a gap is formed between the inner wall of the axial protrusion 43 and the outer wall of the radial protrusion 22. The local thickness of the connecting flange 41 is increased by the axial convex part 43, the strength of the connecting flange 41 is improved, firm connection with the piston body 1 is further realized, meanwhile, a gap is reserved between the inner wall of the axial convex part 43 and the outer wall of the radial convex part 22, and the piston rod 2 can be prevented from being blocked when rotating along the cambered surface at will, and the structure is simple and effective.

As a preferred embodiment, the piston rod 2 is a hollow rod and internally pierced with a sample tube 5. The hollow piston rod 2 can facilitate the sample tube 5 to pass through, and the sample tube 5 does not occupy extra space, so that the structure is more compact and the layout is more reasonable.

Further, in this embodiment, a transition fillet 23 is provided between the inner wall of the piston rod 2 and the second arcuate surface portion 21. The transition fillet 23 prevents scratching the gasket 3 when the piston rod 2 is angularly displaced.

Further, in this embodiment, the piston body 1 is provided with a positioning portion 12, the positioning portion 12 extends into the piston rod 2, the first arc surface portion 11 is located at the outer periphery of the positioning portion 12, and the sealing gasket 3 is sleeved on the positioning portion 12. When the piston rod 2 is angularly displaced, the sealing gasket 3 may be driven to move, the sealing gasket 3 is sleeved on the positioning portion 12, and displacement of the sealing gasket 3 can be effectively prevented, so that good sealing performance is always maintained between the first arc surface portion 11 and the second arc surface portion 21.

Further, in this embodiment, the hydraulic cylinder-free dynamic axial compression chromatographic column further comprises a column tube 6, an upper cover 61 arranged at the upper end of the column tube 6, a lower cover 62 arranged at the lower end of the column tube 6, and a slurry feeding valve 7 arranged on the lower cover 62. Referring specifically to fig. 1, the slurry can be injected into the cavity below the piston body 1 from bottom to top through the slurry feed valve 7, the piston body 1 does not need to be lifted above the column tube 6, the side wall of the column tube 6 does not need to be perforated, and the downward gravity of the slurry in the column tube 6 is opposite to the upward impact force direction of the slurry which is continuously injected, so that the problem that the packing column bed after final filling is uneven in compactness or has 'sugar cores' is solved, and the structure is reasonable.

Further, in this embodiment, the slurry feeding valve 7 includes a valve seat 71 connected to the lower cover 62, a feeding pipe 72 disposed in the valve seat 71, a valve core 73 for blocking the feeding pipe 72, and an opening and closing assembly 74 for driving the valve core 73 to reciprocate in the feeding pipe 72, wherein the upper and lower ends of the outer wall of the feeding pipe 72 are in sealing fit with the valve seat 71, a feeding gap 75 is formed between the middle section and the valve seat 71, a feeding interface 76 is disposed on the valve seat 71 and is communicated with the feeding gap 75, a feeding port 721 is disposed on the side wall of the feeding pipe 72 and a discharging port 722 is disposed on the top and is communicated with the column tube 6. Referring specifically to fig. 3, when the valve core 73 is lifted to the top end of the feed pipe 72, the discharge port 722 at the top of the feed pipe 72 is blocked, and at this time, the homogenate cannot enter the column pipe 6; referring specifically to fig. 5, after the valve core 73 is driven by the opening and closing assembly 74 to move downward, the slurry can be sequentially injected into the column tube 6 through the feeding interface 76, the feeding gap 75, the feeding port 721, the cavity of the feeding pipe 72 positioned on the upper side of the valve core 73, and the discharging port 722, so that the structure is simple and reliable.

Further, in the present embodiment, the opening and closing assembly 74 includes a pneumatic motor 741 connected to the valve seat 71 and a connection nut 742 connected to the pneumatic motor 741, and the valve core 73 has an upper end positioned in the feed pipe 72 and a lower end provided with an external thread and connected to the connection nut 742. When the valve core 73 needs to move in the feeding pipe 72, the pneumatic motor 741 is started to drive the connecting nut 742 to rotate, and the lower end of the valve core 73 is connected with the connecting nut 742, so that the valve core 73 can move along the axial direction relative to the connecting nut 742.

As a preferred embodiment, the upper end of the valve seat 71 extends into the lower cover 62 and a liquid outlet gap 77 is provided between the outer wall and the lower cover 62, and a liquid outlet port 78 communicating with the liquid outlet gap 77 is provided on the valve seat 71. Referring specifically to fig. 4 and 6, after the liquid in the column tube 6 is filtered by the sieve plate, the liquid is discharged through the liquid outlet gap 77 and the liquid outlet interface 78 in sequence. The liquid outlet flow passage is also concentrated on the uniform slurry feeding valve 7, so that the structure is more compact.

While the utility model has been described with reference to preferred embodiments, it is not intended to be limiting. Many possible variations and modifications of the disclosed technology can be made by anyone skilled in the art, or equivalent embodiments with equivalent variations can be made, without departing from the scope of the utility model. Therefore, any simple modification, equivalent variation and modification of the above embodiments according to the technical substance of the present utility model shall fall within the scope of the technical solution of the present utility model.

Claims (10)

1. The utility model provides a no pneumatic cylinder dynamic axial compression chromatographic column, includes piston body (1) and piston rod (2), its characterized in that: the piston rod is characterized by further comprising a sealing gasket (3) arranged between the piston body (1) and the piston rod (2) and a connecting assembly (4) used for movably connecting the piston rod (2) with the piston body (1), a first arc surface part (11) is arranged on the piston body (1), a second arc surface part (21) matched with the first arc surface part (11) is arranged on the piston rod (2), one side of the sealing gasket (3) is tightly attached to the first arc surface part (11), and the other side of the sealing gasket (3) is tightly attached to the second arc surface part (21).

2. The cylinder-free dynamic axial compression chromatography column of claim 1, wherein: the connecting assembly (4) comprises a connecting flange (41) and a plurality of fasteners (42) which are distributed along the circumferential direction of the connecting flange (41) at intervals, a radial convex part (22) is arranged on the piston rod (2), the connecting flange (41) is sleeved on the piston rod (2) and is abutted to the end face of the radial convex part (22), a gap is reserved between the inner wall of the connecting flange (41) and the outer wall of the piston rod (2), and the fasteners (42) are connected with the piston body (1).

3. The cylinder-less dynamic axial compression chromatography column of claim 2, wherein: an axial convex part (43) is arranged on the connecting flange (41), and a gap is reserved between the inner wall of the axial convex part (43) and the outer wall of the radial convex part (22).

4. The cylinder-free dynamic axial compression chromatography column of claim 1, wherein: the piston rod (2) is a hollow rod and is internally provided with a sample tube (5) in a penetrating way.

5. The cylinder-less dynamic axial compression chromatography column of claim 4, wherein: a transition fillet (23) is arranged between the inner wall of the piston rod (2) and the second arc surface part (21).

6. The cylinder-less dynamic axial compression chromatography column of claim 4, wherein: the piston body (1) is provided with a positioning part (12), the positioning part (12) extends into the piston rod (2), the first arc surface part (11) is positioned on the periphery of the positioning part (12), and the sealing gasket (3) is sleeved on the positioning part (12).

7. The hydraulic cylinder free dynamic axial compression chromatography column of any one of claims 1 to 6, wherein: the device also comprises a column tube (6), an upper cover (61) arranged at the upper end of the column tube (6), a lower cover (62) arranged at the lower end of the column tube (6) and a slurry feeding valve (7) arranged on the lower cover (62).

8. The cylinder-free dynamic axial compression chromatography column of claim 7, wherein: the slurry feeding valve (7) comprises a valve seat (71) connected with a lower cover (62), a feeding pipe (72) arranged in the valve seat (71), a valve core (73) for blocking the feeding pipe (72) and an opening and closing assembly (74) for driving the valve core (73) to reciprocate in the feeding pipe (72), feeding gaps (75) are formed between the upper end and the lower end of the outer wall of the feeding pipe (72) and the valve seat (71) in sealing fit, the middle section and the valve seat (71), feeding interfaces (76) communicated with the feeding gaps (75) are formed in the valve seat (71), and a feeding hole (721) communicated with the feeding gaps (75) is formed in the side wall of the feeding pipe (72) and a discharging hole (722) communicated with the column pipe (6) is formed in the top.

9. The cylinder-free dynamic axial compression chromatography column of claim 8, wherein: the opening and closing assembly (74) comprises a pneumatic motor (741) connected with the valve seat (71) and a connecting nut (742) connected with the pneumatic motor (741), the upper end of the valve core (73) is positioned in the feeding pipe (72), and the lower end of the valve core is provided with external threads and is connected with the connecting nut (742).

10. The cylinder-free dynamic axial compression chromatography column of claim 8, wherein: the upper end of the valve seat (71) extends into the lower cover (62), a liquid outlet gap (77) is formed between the outer wall of the valve seat and the lower cover (62), and a liquid outlet interface (78) communicated with the liquid outlet gap (77) is arranged on the valve seat (71).

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