CN219201346U

CN219201346U - Plant tissue slice damage dynamic monitoring device

Info

Publication number: CN219201346U
Application number: CN202223559406.2U
Authority: CN
Inventors: 石梦豪; 朱德泉; 况福明; 熊玮; 张晓双; 耿子轩; 却锦楠; 孙东东; 武立权
Original assignee: Anhui Agricultural University AHAU
Current assignee: Anhui Agricultural University AHAU
Priority date: 2022-12-30
Filing date: 2022-12-30
Publication date: 2023-06-16
Anticipated expiration: 2032-12-30

Abstract

The utility model discloses a plant tissue slice damage dynamic monitoring device which comprises a microscope, wherein an extrusion device is arranged right in front of a microscope seat of the microscope, the extrusion device comprises a supporting fixing frame, a sliding block which moves back and forth is arranged on the supporting fixing frame, a three-dimensional force sensor is arranged on the sliding block, a force measuring component which is used for touching a slice to be analyzed on an extrusion object stage and transmitting force to the three-dimensional force sensor is arranged on the three-dimensional force sensor, and an image pickup unit is arranged on one side of the microscope seat of the microscope. According to the utility model, the movable force measuring device with the three-dimensional force sensor, the microscope and the camera unit are used for extruding a sample on the microscope, so that the problem that the damage of the plant tissue slice cannot be detected at the first time is solved, the damage process of the plant tissue slice can be known more clearly through the high-speed camera, and the corresponding relation between the damage state and the force of the plant tissue slice in the whole damage process can be obtained more clearly through the numerical value fed back by the three-dimensional force sensor.

Description

Plant tissue slice damage dynamic monitoring device

Technical Field

The utility model relates to plant tissue slice damage detection, in particular to a plant tissue slice damage dynamic monitoring device.

Background

The traditional plant tissue slice damage detection device mainly comprises the steps of extruding plant tissues by a texture analyzer, then making plant tissue slices, statically observing the damaged plant tissue slices by a microscope, and analyzing the damage condition of the damaged plant tissue slices, wherein when the plant tissue slices are cut, the plant tissues are secondarily damaged, the plant tissues are extruded firstly, then the plant tissues are observed, and the obtained observation result cannot reflect the condition that the plant tissue slices are damaged for the first time.

In order to realize the dynamic process of detecting the damage at the first time after the damage of the plant tissue and analyze the damage condition of the plant tissue, the detection method conventionally used for the plant tissue section, such as extrusion and then section observation, is not applicable. For implementing dynamic detection, patent CN2020228902426 discloses a novel pressure sensing device, which comprises a control unit, a wireless communication unit and a pressure sensor, wherein the pressure sensor comprises a shell, a baroreceptor, a transmission elastic sheet, a resistance strain gauge, a probe mounting seat and a signal probe, and the functions of pressure detection and transmission are realized by matching the baroreceptor, the transmission elastic sheet, the resistance strain gauge and the signal probe.

Based on the principle of this patent, this application combines microscope and camera unit through mobilizable, has three-dimensional force transducer's force measuring device, realizes the extrusion to the sample on the microscope, solves the problem that can not detect plant tissue section damage in the first time, through the high-speed camera of installation, can know plant tissue section's damage process more clearly to through the numerical value of three-dimensional force transducer feedback, can obtain the corresponding relation of object tissue section damage state and power in whole damage in-process more clearly.

Disclosure of Invention

The utility model aims to overcome the defects and the shortcomings of the prior art, and provides a plant tissue slice damage dynamic monitoring device which solves various problems in the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

the utility model provides a plant tissue section damage dynamic monitoring device, is including the microscope, install extrusion device in the microscope's microscope stand dead ahead, extrusion device is including supporting the mount, install a horizontal lead screw that sets up towards microscope objective table direction on the support mount, install reciprocating motion's slider on the horizontal lead screw, install three-dimensional force sensor on the slider, install the dynamometry subassembly that is used for touching the analysis section on the extrusion objective table and gives three-dimensional force sensor with the force transmission, the dynamometry subassembly is including the probe, the afterbody of probe is through foil gage fixed mounting on the terminal surface towards microscope three-dimensional force sensor, camera shooting unit is installed to microscope stand one side.

The support fixing frame comprises a base, a sliding rail support is arranged in the middle of the base, a sliding seat is arranged on the sliding rail support, fixing supports used for fixing two ends of a horizontal screw rod are respectively arranged at two ends of the sliding seat, the horizontal screw rod is driven by a motor at the end part, and the motor is arranged at the end far away from the microscope.

The sliding block moves to be guided by the guide assembly at the bottom end, the guide assembly comprises two sliding rails which are arranged on two sides of the sliding seat in parallel, a sliding groove matched with the sliding rail guiding groove is formed in the bottom end of the sliding block, and a guide hole screwed with the horizontal screw rod is formed in the middle of the sliding block.

The upper end face middle part of slider is equipped with the draw-in groove, three-dimensional force transducer's bottom card dress is in the draw-in groove, and upper end protrusion slider up end.

The camera unit comprises a camera base, a fixing frame is fixedly arranged on the camera base, a fixing clamp used for clamping the high-speed camera is arranged at the tail part of the upper end of the fixing frame, and the fixing frame is connected with the fixing clamp through an angle-adjustable ball joint connecting component.

The fixing clamp comprises a first arc fixing piece and a second arc fixing piece which are correspondingly matched with each other through beams, corresponding lug plates are respectively arranged on two sides of the first arc fixing piece and the second arc fixing piece, and the lug plates are fixedly connected through bolts in a locking mode.

The ball head connecting assembly comprises a ball head which is arranged at the tail end of a fixing frame and the outer end part of an arc-shaped fixing piece I respectively, the ball heads are clamped and fixed through a pipe clamp, the pipe clamp is connected by two involuted half pipes and an adjusting locking pin between the half pipes, the end part of the adjusting locking pin is provided with a knob, and two ends of the half pipes are respectively provided with a concave cavity matched with the ball head.

Compared with the prior art, the utility model has the beneficial effects that:

(1) The motor is arranged to drive the screw rod to rotate, so that the slide block and the force measuring device do linear reciprocating motion, lateral extrusion of samples on a microscope is realized, the traditional process of extruding plant tissues firstly and then observing the slices is avoided, and the problem that damage to the plant tissue slices cannot be detected at the first time is solved.

(2) Through placing the camera unit, can know plant tissue section's damage process in real time more clearly to through the numerical value of three-dimensional force sensor feedback, can obtain plant tissue section in the corresponding relation of whole damage in-process damage state and power more clearly.

Drawings

Fig. 1 is a schematic structural diagram of a first embodiment of the present utility model.

Fig. 2 is a schematic structural view of the force measuring assembly.

Fig. 3 is a schematic structural diagram of a second embodiment of the present utility model.

Fig. 4 is a schematic structural view of the connection between the fixing clip and the fixing frame through the ball head connecting assembly.

Reference numerals:

1. a base; 2. a slide rail bracket; 3. an image pickup unit; 4. a high-speed camera; 5. a camera base; 6. a slide; 7. a motor; 8. adjusting the locking pin; 9. a horizontal screw rod; 10. a slide block; 11. a three-dimensional force sensor; 12. a force measuring assembly; 13. a microscope; 14. a strain gage; 15. a probe; 16. a slide rail; 17. ball head; 18. a pipe clamp; 19. a fixing clamp; 19-1, an arc-shaped fixing piece I; 19-2, an arc-shaped fixing piece II; 20. a fixing frame; 21. and (5) a knob.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Embodiment one:

as shown in figures 1-2; the utility model provides a plant tissue section damage dynamic monitoring device, including microscope 13, install extrusion device in the microscope 13's microscope stand dead ahead, extrusion device is including supporting the mount, install a horizontal lead screw 9 that sets up towards microscope objective table direction on the support mount, install reciprocating motion's slider 10 on the horizontal lead screw 9, and the guide assembly direction through the bottom is removed to slider 10, install three-dimensional force sensor 11 on the slider 10, install the measurement force subassembly 12 that is used for touching the slice of waiting to analyze on the extrusion objective table and gives three-dimensional force sensor 11 with the force, measurement force subassembly 12 is including probe 15, the afterbody of probe 15 passes through foil gage 14 fixed mounting on the terminal surface of three-dimensional force sensor 11 towards microscope 13, camera unit 3 is installed to microscope 13's microscope stand one side. The sliding block 10 and the force measuring component 12 are driven by the horizontal screw rod to do linear reciprocating motion, so that the extrusion of plant tissue slices is realized, meanwhile, the three-dimensional force sensor 11 is connected with a computer, and the measured force value is fed back to the computer.

Further, the support mount is including base 1, and the mid-mounting of base 1 has slide rail support 2, installs slide 6 on the slide rail support 2, and the fixed bolster that is used for fixed horizontal lead screw both ends is installed respectively at the both ends of slide 6, and horizontal lead screw 9 is driven by the motor 7 of tip, and motor 7 is installed and is keeping away from the microscope end.

The guide assembly comprises two slide rails 16 which are arranged on two sides of the slide seat 6 in parallel, a slide groove matched with the slide rail 16 is arranged at the bottom end of the slide block 10, and a guide hole screwed with a horizontal screw rod is arranged in the middle of the slide block 10. The base is used as a bottom foundation for supporting the fixing frame, the sliding rail support is used for supporting and erecting the horizontal screw rod, the motor acts to drive the horizontal screw rod to rotate, and meanwhile, the sliding blocks are under the guiding action of the sliding rails at the bottoms of the two sides, so that the horizontal screw rod can move back and forth along the horizontal direction. If the sliding rail support of fig. 1 is of a U-shaped structure, the top and the bottom of the sliding rail support are respectively provided with four threaded holes, the top of the sliding rail support is connected with the sliding seat through screws, the bottom of the sliding rail support is connected with the base through bolts, and the supporting erection of the sliding rail support and the sliding seat is realized.

The specific application process of the structure is as follows: when the device is in practical application, a sample is placed on a microscope, the rotating speed of a motor is regulated, so that a horizontal screw rod rotates, a sliding block is driven to do reciprocating linear motion, a three-dimensional force sensor is arranged in a groove above the sliding block, a force measuring component is arranged on the three-dimensional force sensor, the sample is extruded, the microscope is externally connected with a computer, the damage process of a plant tissue slice can be observed in real time, meanwhile, a high-speed camera is placed on one side of a base of the extruding device, the whole extruding process can be recorded in a whole process, the high-speed camera is externally connected with the computer, a shot extruding video can be transmitted to the computer, the three-dimensional force sensor is externally connected with the computer, the measured force values are fed back to the computer, and meanwhile, the values and the extruding process are recorded in a corresponding relation between the dynamic process of the damage of the plant tissue slice and acting force corresponding to the dynamic monitoring of the damage of the plant tissue slice is achieved.

Embodiment two:

as shown in fig. 3-4; the utility model provides a plant tissue section damage dynamic monitoring device, including microscope 13, install extrusion device in the microscope 13's microscope stand dead ahead, extrusion device is including reciprocating motion's slider 10, install three-dimensional force transducer 11 on the slider 10, install the dynamometry subassembly 12 that is used for touching the slice of waiting to analyze on the extrusion objective table and transmits the force to three-dimensional force transducer 11 on the three-dimensional force transducer 11, dynamometry subassembly 12 is including probe 15, probe 15's afterbody passes through foil gage 14 fixed mounting on the terminal surface towards microscope 13's three-dimensional force transducer 11, camera unit 3 is installed to microscope 13's microscope stand one side.

Wherein: the camera unit 3 comprises a camera base 5, a fixing frame 20 is fixedly arranged on the camera base 5, a fixing clamp 19 for clamping the high-speed camera 4 is arranged at the tail part of the upper end of the fixing frame 20, and the fixing frame 20 and the fixing clamp 19 are connected through an angle-adjustable ball joint connecting component. Through the structure of fixation clamp, be suitable for the high-speed camera of different grade type, realized the angular adjustment between mount and the fixation clamp through bulb coupling assembling's structure, when observing sample extrusion process, adjust the fixation clamp to suitable position through bulb coupling assembling, then with fixation clamp centre gripping high-speed camera for the high-speed camera adjusts suitable angle, then shoots, and high-speed camera connection computer simultaneously, with video transmission for the computer after shooting.

Further, the fixing clamp 19 comprises a first arc fixing piece 19-1 and a second arc fixing piece 19-2 which are correspondingly matched with each other through beams, corresponding lug plates 19-3 are respectively arranged on two sides of the first arc fixing piece 19-1 and the second arc fixing piece 19-2, and the lug plates 19-3 are connected and fixed through bolt locking 19-4. The bulb connecting assembly comprises a bulb 17 which is arranged at the tail end of a fixing frame and the outer end part of an arc-shaped fixing piece I respectively, the bulbs 17 are clamped and fixed through a pipe clamp 18, the pipe clamp 18 is connected by two involuted half pipes and an adjusting locking pin 8 between the half pipes, the end part of the adjusting locking pin 8 is provided with a knob 21, and two ends of the half pipes are respectively provided with a concave cavity matched with the bulbs 17.

The clamping of the high-speed camera is realized through the fixing clamp, the clamping space between the first arc-shaped fixing piece and the second arc-shaped fixing piece of the fixing clamp can be adjusted according to bolts on two sides, so that the requirements of clamping and fixing of the high-speed cameras with different specifications are met, rubber pads can be arranged on the inner side surfaces of the clamping parts of the first arc-shaped fixing piece and the second arc-shaped fixing piece, on one hand, the clamping is more stable, and on the other hand, the clamping part of the high-speed camera can be protected, and scratch is avoided; through the structure of bulb coupling assembling, can adjust the high-speed camera to suitable angle, then shoot.

Although the present disclosure describes embodiments, not every embodiment is described in terms of a single embodiment, and such description is for clarity only, and one skilled in the art will recognize that the embodiments described in the disclosure as a whole may be combined appropriately to form other embodiments that will be apparent to those skilled in the art.

Therefore, the above description is only of the preferred embodiments of the present application and is not intended to limit the scope of the present application; all changes which come within the meaning and range of equivalency of the claims are to be embraced within their scope.

Claims

1. Plant tissue section damage dynamic monitoring device, including microscope (13), its characterized in that: the device is characterized in that an extruding device is arranged right in front of a microscope base of the microscope (13), the extruding device comprises a supporting fixing frame, a horizontal screw rod (9) which is arranged towards the direction of a microscope stage is arranged on the supporting fixing frame, a sliding block (10) which moves back and forth is arranged on the horizontal screw rod (9), a three-dimensional force sensor (11) is arranged on the sliding block (10), a force measuring component (12) which is used for touching a slice to be analyzed on the extruding stage and transmitting force to the three-dimensional force sensor (11) is arranged on the three-dimensional force sensor (11), the force measuring component (12) comprises a probe (15), the tail of the probe (15) is fixedly arranged on the end face of the three-dimensional force sensor (11) which faces the microscope (13) through a strain gauge (14), and a camera shooting unit (3) is arranged on one side of the microscope base of the microscope (13).

2. The plant tissue slice injury dynamic monitoring device of claim 1, wherein: the support fixing frame comprises a base (1), a sliding rail support (2) is arranged in the middle of the base (1), a sliding seat (6) is arranged on the sliding rail support (2), fixing supports used for fixing two ends of a horizontal screw rod are respectively arranged at two ends of the sliding seat (6), the horizontal screw rod (9) is driven by a motor (7) at the end part, and the motor (7) is arranged at the end far away from the microscope.

3. The plant tissue slice injury dynamic monitoring device of claim 1, wherein: the sliding block (10) moves to be guided by a guide assembly at the bottom end, the guide assembly comprises two sliding rails (16) which are arranged at two sides of the sliding seat (6) in parallel, a sliding groove matched with the sliding rail (16) is formed in the bottom end of the sliding block (10), and a guide hole screwed with a horizontal screw rod is formed in the middle of the sliding block (10).

4. A plant tissue slice injury dynamic monitoring device according to claim 1 or 3, wherein: the middle part of the upper end face of the sliding block (10) is provided with a clamping groove, the bottom end part of the three-dimensional force sensor (11) is clamped in the clamping groove, and the upper end part of the three-dimensional force sensor protrudes out of the upper end face of the sliding block (10).

5. The plant tissue slice injury dynamic monitoring device of claim 1, wherein: the camera shooting unit (3) comprises a camera base (5), a fixing frame (20) is fixedly installed on the camera base (5), a fixing clamp (19) used for clamping the high-speed camera (4) is installed at the tail of the upper end of the fixing frame (20), and the fixing frame (20) and the fixing clamp (19) are connected through an angle-adjustable ball joint connecting assembly.

6. The plant tissue slice injury dynamic monitoring device of claim 5, wherein: the fixing clamp (19) comprises a first arc-shaped fixing piece (19-1) and a second arc-shaped fixing piece (19-2) which are correspondingly matched with each other through beams, corresponding lug plates (19-3) are respectively arranged on two sides of the first arc-shaped fixing piece (19-1) and the second arc-shaped fixing piece (19-2), and the lug plates (19-3) are fixedly connected through bolt locking (19-4).

7. The plant tissue slice injury dynamic monitoring device of claim 6, wherein: the ball head connecting assembly comprises a ball head (17) which is arranged at the tail end of a fixing frame and the outer end part of an arc fixing piece I respectively, the ball heads (17) are clamped and fixed through a pipe clamp (18), the pipe clamp (18) is connected by two involuted half pipes and an adjusting locking pin (8) between the half pipes, the end part of the adjusting locking pin (8) is provided with a knob (21), and two ends of the half pipes are respectively provided with a concave cavity matched with the ball heads (17).