CN219146577U

CN219146577U - Cell cryopreservation frame

Info

Publication number: CN219146577U
Application number: CN202222313396.8U
Authority: CN
Inventors: 马丽雅; 方晓; 谢海涛
Original assignee: Shenzhen Xiankangda Life Science Co ltd
Current assignee: Shenzhen Xiankangda Life Science Co ltd
Priority date: 2022-08-30
Filing date: 2022-08-30
Publication date: 2023-06-09
Anticipated expiration: 2032-08-30

Abstract

The utility model discloses a cell cryopreservation frame, which comprises a rotating shaft rod, wherein a plurality of space layers which are arranged in a vertically stacked mode are sleeved on the rotating shaft rod in a penetrating way, and a cryopreservation box is arranged on each space layer in an adaptive mode; each of the space layers is rotatable about the shaft in a clockwise or counterclockwise direction relative to the horizontal plane. The cell freezing frame adopts the space layer to rotate relative to the rotating shaft rod, and the freezing between the space layer of the freezing frame and the freezing box is broken through rotating torsion, so that the freezing box is rapidly taken out from the space layer of the freezing frame; the freezing and storing efficiency is high, deicing in the air is not required to be exposed for a long time, and the biological activity of the frozen cells is preserved.

Description

Cell cryopreservation frame

Technical Field

The utility model relates to the field of preparation of cell cryopreservation devices, in particular to a cell cryopreservation frame.

Background

The freezing and preserving of cells, including stem cells, immune cells, CAR-T cells and the like, is carried out at the present stage by placing a freezing tube containing freezing solution and cells on a freezing box, then placing the freezing box on a freezing frame and placing the freezing box in a liquid nitrogen tank for freezing and preserving, for example, the utility model patent CN20577446U (patent name: a portable cell freezing frame); the freezing frame is provided with a plurality of upper and lower structured space layers, each space layer can be used for placing a freezing box, and the freezing box and the freezing frame are placed in a horizontal drawing mode. However, the freezing box and the freezing frame are frozen or frosted, so that the freezing box cannot be taken out in a short time, and the freezing box can be taken out from the freezing frame only after the ice is dissolved; in the process of freezing or frosting and dissolving, all cells on the freezing shelf need to be exposed to the air for a long time, and at this time, the biological activity of the frozen cells is affected.

Disclosure of Invention

Based on the above problems, the present utility model aims to provide a cell freezing frame which can rapidly take out a freezing box from the freezing frame without affecting the biological activity of frozen cells.

The technical scheme of the utility model is as follows:

the cell cryopreservation frame comprises a rotating shaft rod, wherein a plurality of space layers which are arranged in a vertically stacked mode are sleeved on the rotating shaft rod in a penetrating mode, and a cryopreservation box is arranged on each space layer in an adaptive mode; each space layer can rotate clockwise or anticlockwise relative to the horizontal surface of the rotating shaft rod.

In one embodiment, in the cell cryopreserving rack, each space layer is formed by a bottom plate, a left side plate, a right side plate, a rear side plate and a middle through pipe column into an open drawer frame structure, and the middle through pipe column is arranged at the joint of the rear side plate and the right side plate or at the joint of the rear side plate and the left side plate; the space layer rotates relative to the rotating shaft rod through the middle through pipe column.

In one embodiment, the cell cryopreservation frame further comprises a plurality of limiting pieces; each limiting piece comprises a fixed end and an extending end; correspondingly, a limiting hole is formed in the joint of the rear side plate, the middle pipe column and the right side plate or the left side plate of each space layer; the fixed end of each limiting piece is fixedly arranged on the rotating shaft rod through a limiting hole formed in the space layer, and the extending end of each limiting piece horizontally extends to the outer wall of the rear side plate of the space layer and is used for blocking the space layer from rotating circularly relative to the rotating shaft rod.

In one embodiment, the cell cryopreservation frame further comprises a plurality of limiting pieces; each limiting piece comprises a fixed end and an extending end; correspondingly, a limiting hole is formed in the joint of the rear side plate, the middle pipe column and the right side plate or the right side plate of each space layer; the fixed end of each limiting piece is fixedly arranged on the rotating shaft rod through a limiting hole formed in the space layer, and the extending end of each limiting piece horizontally extends to the outer wall of the rear side plate of the space layer and is used for blocking the space layer from rotating circularly relative to the rotating shaft rod.

According to the cell freezing and storing frame provided by the utility model, the space layer is rotated relative to the rotating shaft rod, and the freezing between the space layer of the freezing and storing frame and the freezing and storing box is broken through rotating torsion, so that the freezing and storing box is rapidly taken out from the space layer of the freezing and storing frame; the freezing and storing efficiency is high, deicing in the air is not required to be exposed for a long time, and the biological activity of the frozen cells is preserved.

Drawings

FIG. 1 is a schematic view of the front structure of a freezing shelf of the present utility model;

FIG. 2 is a schematic view of the back structure of the freezing shelf of the present utility model;

FIG. 3 is a schematic view of a spatial layer structure in a freezing shelf;

fig. 4 is a schematic view of a limiting member structure in the freezing shelf;

FIG. 5 is an enlarged partial schematic view of portion A of FIG. 3;

FIG. 6 is a partial cross-sectional view of the latch structure (after release) on the spatial layer;

FIG. 7 is a partial cross-sectional view of the latch structure (after latching) on the spatial layer;

FIG. 8 is a schematic view of the structure of the freezing box;

FIG. 9 is a schematic diagram of the structure of a cell cryopreservation tube.

Detailed Description

The preferred embodiments of the present utility model will be described in further detail with reference to the accompanying drawings.

As shown in fig. 1, 2, 8 and 9, the cell cryopreservation frame 10 provided by the utility model comprises a rotating shaft rod 100, wherein a plurality of space layers 200 which are arranged in a vertically stacked manner are sleeved on the rotating shaft rod 100, and a cryopreservation box 300 is adaptively arranged on each space layer 200; each of the space layers 200 may be rotated clockwise (I direction) or counterclockwise (II direction) about the shaft 100 with respect to the horizontal plane. The freezing box 300 is provided with a plurality of placement holes 310 which are arranged in an array and used for placing freezing pipes 320.

The rotating shaft rod 100 is in a cylindrical or cylindrical tubular structure, and the rotating shaft rod 100 is made of medical grade stainless steel materials and aluminum alloy materials.

As shown in fig. 3, the space 200 is formed by a bottom plate 204, a left side plate 201, a right side plate 202, a rear side plate 203, and a middle through pipe column 205 into an open drawer frame structure; in this embodiment, the middle tube column 205 is disposed at the connection between the rear side plate 203 and the right side plate 202, and in another embodiment, the middle tube column 205 is disposed at the connection between the rear side plate 203 and the left side plate 201. The inner diameter of the middle through pipe column 205 is matched with the outer diameter of the rotating shaft rod 100, and the space layer 200 is sleeved on the rotating shaft rod 100 through the middle through pipe column 205 in a fit manner, so that the space layer 200 rotates clockwise (I direction) or anticlockwise (II direction) around the rotating shaft rod 100 relative to the horizontal surface. In order to make the space layer 200 rotate smoothly around the shaft 100, generally, the inner diameter of the middle tube column 205 is slightly larger than the outer diameter of the shaft 100, i.e. the inner diameter of the middle tube column 205 is 1.2-1.2 times the outer diameter of the shaft 100, so that the rotation space is slightly surplus and the rotation is smoother.

As shown in fig. 2 to 4, preferably, in order to prevent 360 ° endless rotation of the space layers 200 around the rotation shaft 100, a limiting structure is required to prevent 360 ° endless rotation of each space layer 200 with respect to the rotation shaft 100; therefore, the present utility model designs the stoppers 120, and each of the space layers 200 corresponds to one stopper 120. The limiting member 120 includes a fixed end 122 and an extending end 121, in this embodiment, the fixed end 122 is in an arc-shaped structure, and the radius of the arc is adapted to the outer diameter of the rotating shaft 100, so that the fixed end 122 can be ensured to be adapted to be clamped into the outer wall of the rotating shaft 100, and the limiting member is fixed on the rotating shaft 100 through a fastening screw 124 passing through a fastening hole 123 provided on the fixed end 122. The extending end 121 of the limiting member 120 extends horizontally and is adapted to abut against the outer wall of the rear side plate 203 of the space layer 200, thereby preventing 360 ° infinite circular rotation of the space layer 200 relative to the rotating shaft 100. Correspondingly, a limiting hole 2051 is formed at the joint between the middle through pipe column 205, the rear side plate 202 and the right side plate 201 (or the left side plate 201) of each space layer 200; the fixed end 122 of the limiting piece 120 is fixedly arranged on the rotating shaft rod 100 through the limiting hole 2051, and the extending end 120 of the limiting piece 120 horizontally extends to the outer wall of the rear side plate 203 to prevent the space layer 200 from infinitely rotating at 360 degrees relative to the rotating shaft rod 100; in this way, it is convenient to make a certain space layer 200 exhibit a certain offset with respect to other space layers 200, so as to more conveniently take out the cryopreservation cassette 300 of the space layer 200.

As shown in fig. 1, when a certain spatial layer 200 needs to be opened, a torque is manually applied anticlockwise (in direction II) to rotate the spatial layer anticlockwise by a certain angle relative to the rotating shaft lever 100, and the spatial layer 200 is rotated to obtain a position corresponding to a dotted spatial layer 200 '(here, for explaining an imaginary position represented by the spatial layer after rotation, to show that the physical spatial layer is distinguished, so that the dotted spatial layer 200' is adopted, in fact, the spatial layer 200 is also used, and no other meaning is given); continuing to rotate, the stopper 120 blocks the right side plate 202 or the left side plate 201 of the space layer, preventing 360 ° rotation of the space layer 200 in the counterclockwise direction. Thus, by the relative rotation of the space layer 200 with respect to the rotating shaft 100, the frozen ice cubes on the space layer 200 and the freezing box 300 can be easily shaken off, and the freezing box 300 on the space layer 200 can be taken out.

As shown in fig. 2, when the space layer 200 needs to return, a torque force is manually applied clockwise (I direction) at this time to rotate the space layer 200 clockwise relative to the rotating shaft 100, and the middle 200 stops at a preset position due to the blocking effect of the limiting member 120, so that 360 ° rotation of the space layer 200 clockwise is prevented.

Preferably, the cell freezing shelf 10 further comprises a shielding cover 110, and the shielding cover 110 is disposed above the uppermost space layer 200 and fixedly disposed on the rotating shaft lever 100. The shielding cover 100 can prevent dust, foreign matter, etc. from contaminating or damaging the freezing tube 320 or the freezing box 300 on the uppermost space layer 200.

Preferably, a reverse bending hook 111 is arranged at the top end of the rotating shaft rod 100, and the cell freezing and storing frame 10 is hung at the opening end of the liquid nitrogen tank through the hook 111, so that the cell freezing and storing frame 10 can be conveniently taken.

Further, as shown in fig. 3, a latch structure 202 is provided at a side 213 of the opening 210 of each space layer 200; the locking structure 202 is used for locking the freezing storage box 300 placed on the space layer 200; thus, the latch structure 202 can function to prevent the cryopreservation cassette 300 from sliding out of the space layer 200 when the cell cryopreservation frame 10 is moved.

Specifically, as shown in fig. 3, 5, 6 and 7, the latch structure 202 includes a latch post 217, a vertical chute 216, and an elastic member 2192. The vertical sliding groove 216 is disposed on the side 213 of the opening 210 of the space layer 200, and is vertically opened downward from the upper surface of the side 213 of the opening 210 of the space layer 200, so as to form a countersink structure that does not penetrate the bottom surface of the side 213. The depth of the vertical sliding groove 216 is 1.1-1.2 times of the height of the locking column 217, so that the locking column 217 can be hidden in the vertical sliding groove 216 after being retracted into the vertical sliding groove 216, and the freezing box 300 is convenient to take out. The locking posts 217 and the elastic members 2192 are disposed in the vertical sliding slots 216 sequentially from top to bottom. In an initial state, that is, when the locking structure 202 locks the freezing box 300, the elastic component 2192 is in a pressed state, and the locking post 217 is pushed out by the restoring force of the elastic component 2192 to lock the freezing box 300; when the freezing box 300 needs to be taken out, the locking post 217 is pressed downwards by external force, the locking post 217 is retracted into the vertical sliding groove 216, and the freezing box 300 is taken out.

Preferably, as shown in fig. 5, 6 and 7, a clamping post 218 is provided on the outer wall of the locking post 217, and the clamping post 218 is perpendicular to the locking post 217; the side 213 of the opening 210 of the space layer 200 is also provided with a vertical limit groove 214 and a horizontal clamping groove 215, and the vertical limit groove 214 and the horizontal clamping groove 215 are mutually vertical; the clamping column 218 can move back and forth between the vertical limiting groove 214 and the horizontal clamping groove 215, and the length dimension of the vertical sliding groove 216 is 1.5-2 times of the length dimension of the locking column 217, so that a space for the locking column 217 to move left and right in the vertical sliding groove 216 can be provided; meanwhile, an elongated auxiliary sliding piece 2191 is further disposed between the locking post 217 and the elastic member 2192, and the auxiliary sliding piece 2191 is laterally disposed in the vertical sliding slot 216. The auxiliary slide 2191 mainly reduces frictional resistance by sliding the lock post 217 laterally, so that the lock post 217 slides laterally in the vertical slide groove 216 more smoothly.

When it is desired to lock the cryopreservation cassette 300 to the spatial layer 200 of the cell cryopreservation scaffold 10; pushing the clamping column 218 out of the transverse clamping groove 215 to the vertical limiting groove 214 by means of external force; simultaneously, the locking column 217 slides to the left from the right of the vertical chute 216 by means of the auxiliary slide sheet 2191, and then the locking column 217 is ejected upwards by means of the elastic restoring force of the elastic component 2192 to lock the freezing box 300; conversely, when the frozen box 300 needs to be taken out, the clamping column 218 is pressed down by external force, then the clamping column 218 is slid to the right side from the left side of the transverse clamping groove 215, and meanwhile the locking column 217 is slid to the right side from the left side of the vertical sliding groove 216 through the auxiliary sliding sheet 2191, so that the locking column 217 is locked to be ejected upwards, and unlocking and releasing are realized.

In the present utility model, the elastic member 2192 is a coil spring, and the number thereof is two. In other embodiments, the elastic member 2192 may be a spring.

It is to be understood that the foregoing description of the preferred embodiments of the utility model is not to be considered as limiting the scope of the utility model, which is defined by the appended claims.

Claims

1. The cell cryopreservation frame is characterized by comprising a rotating shaft rod, wherein a plurality of space layers which are arranged in a vertically stacked mode are sleeved on the rotating shaft rod in a penetrating mode, and a cryopreservation box is arranged on each space layer in an adaptive mode; each space layer can rotate clockwise or anticlockwise relative to the horizontal surface around the rotating shaft rod.

2. The cell cryopreservation rack of claim 1, wherein each of the spatial layers is composed of a bottom plate, a left side plate, a right side plate, a rear side plate, and a middle through pipe column in an open drawer frame structure, and the middle through pipe column is disposed at a junction of the rear side plate and the right side plate or at a junction of the rear side plate and the left side plate; the space layer rotates relative to the rotating shaft rod through the middle through pipe column.

3. The cell cryopreservation frame of claim 2, further comprising a plurality of limiting members; each limiting piece comprises a fixed end and an extending end; correspondingly, a limiting hole is formed in the joint of the rear side plate, the middle through pipe column and the right side plate of each space layer; the fixed end of each limiting piece is fixedly arranged on the rotating shaft rod through a limiting hole formed in the space layer, and the extending end of each limiting piece horizontally extends to the outer wall of the rear side plate of the space layer and is used for blocking the space layer from rotating circularly relative to the rotating shaft rod.

4. The cell cryopreservation frame of claim 2, further comprising a plurality of limiting members; each limiting piece comprises a fixed end and an extending end; correspondingly, a limiting hole is formed in the joint of the rear side plate, the middle through pipe column and the left side plate of each space layer; the fixed end of each limiting piece is fixedly arranged on the rotating shaft rod through a limiting hole formed in the space layer, and the extending end of each limiting piece horizontally extends to the outer wall of the rear side plate of the space layer and is used for blocking the space layer from rotating circularly relative to the rotating shaft rod.