CN219136769U - Blood agar plate - Google Patents
Blood agar plate Download PDFInfo
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- CN219136769U CN219136769U CN202320114702.7U CN202320114702U CN219136769U CN 219136769 U CN219136769 U CN 219136769U CN 202320114702 U CN202320114702 U CN 202320114702U CN 219136769 U CN219136769 U CN 219136769U
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Abstract
The utility model relates to the technical field of microorganism culture, in particular to a blood agar plate, which comprises a blood agar plate vessel lower shell, a blood agar plate culture substrate, a blood agar plate vessel upper shell and a temperature control culture device, wherein a connecting step is arranged on the outer side of the blood agar plate vessel lower shell, the blood agar plate vessel upper shell is buckled on the connecting step, and the blood agar plate vessel upper shell is also in buckle connection with the blood agar plate vessel lower shell. According to the utility model, the connecting steps and the buckling connection between the upper shell of the blood agar plate vessel and the lower shell of the blood agar plate vessel are arranged, so that the possibility that the upper shell of the blood agar plate vessel is loose due to loose buckling can be eliminated; through setting up accuse temperature culture apparatus, can realize the temperature control culture function to blood agar plate culture substrate to whether it is qualified to the inspection, and then carries out subsequent use.
Description
Technical Field
The utility model relates to the technical field of microorganism culture, in particular to a blood agar plate.
Background
The culture medium refers to a nutrient medium which is used for the growth and reproduction of microorganisms, plants or animals (or tissues) and is prepared by combining different nutrient substances. Generally contains carbohydrate, nitrogenous substances, inorganic salts (including trace elements), vitamins, water and other substances. The culture medium is a basic substance for providing cell nutrition and promoting cell proliferation, and is also a living environment for cell growth and proliferation. The culture medium is of various types and can be classified into a natural culture medium, a synthetic culture medium and a semisynthetic culture medium according to the sources of the preparation raw materials; according to the physical state, it can be classified into solid medium, liquid medium, and semisolid medium; according to the culture function, the culture medium can be divided into basic culture medium, selective culture medium, enrichment culture medium, identification culture medium and the like; according to the application range, bacteria culture medium, actinomycete culture medium, yeast culture medium, fungi culture medium, etc. can be classified.
Blood agar plate culture medium, which is commonly named as blood agar plate culture medium, is used for separating streptococcus and other causticizing bacteria, culturing and detecting hemolytic activity.
Existing blood agar plates, for example: the Chinese patent with publication number of CN110819570A discloses a blood agar plate and a preparation method thereof, which contain various substances such as tryptone, soybean peptone and the like, but the blood agar plate has the problems of inconvenience in separation contrast culture in a vessel and inconvenience in operation and use. In view of the above, chinese patent publication No. CN217757458U discloses a blood agar plate, which divides the blood agar plate culture substrate into two parts by providing a partition plate, so as to observe the growth conditions on both sides of the blood agar plate culture substrate, and provide convenience for adding bacterial liquid material by matching with the use of a storage rack.
However, the disclosed blood agar plate has some defects, such as the blood agar plate needs to be cultured for 24-36 hours at 30-35 ℃ after being prepared and whether the blood agar plate is qualified or not is checked for subsequent use, and the disclosed blood agar plate lacks a temperature control culture device and cannot realize a temperature control culture function; and for example, the upper shell of the blood agar plate vessel is only buckled and connected with the lower shell of the blood agar plate vessel, so that the upper shell of the blood agar plate vessel can be loose due to loose buckling, and the practicability is poor.
For this purpose, a blood agar plate is proposed.
Disclosure of Invention
The utility model aims to overcome the defects of the prior art and provide a blood agar plate with high structural stability and capable of realizing the temperature control culture function.
In order to achieve the above object, the technical scheme of the present utility model is as follows:
the utility model provides a blood agar plate, includes blood agar plate ware inferior valve, blood agar plate culture substrate, blood agar plate ware epitheca, division board and accuse temperature culture apparatus, and blood agar plate culture substrate fills the inside at blood agar plate ware inferior valve, division board integrated glue joint in the inboard intermediate position of blood agar plate ware inferior valve, the outside of blood agar plate ware inferior valve is equipped with the connection step, blood agar plate ware epitheca lock is on the connection step, blood agar plate ware epitheca still is connected with blood agar plate ware inferior valve buckle, blood agar plate ware inferior valve and blood agar plate ware epitheca all adopt transparent material to make, blood agar plate box is constituteed to blood agar plate ware inferior valve, blood agar plate culture substrate and blood agar plate ware epitheca, blood agar plate box is placed in accuse temperature culture apparatus inside.
Preferably, the lower shell of the blood agar plate vessel and the upper shell of the blood agar plate vessel are both made of polystyrene materials.
Preferably, the side of the upper shell of the blood agar plate vessel is hinged with a clamping piece through a hinge seat, and the side of the lower shell of the blood agar plate vessel is provided with a clamping block matched with the clamping piece.
Preferably, the blood agar plate box has a cylindrical structure.
Preferably, the temperature control culture device comprises a shell and a controller arranged on the outer side wall of the shell, the upper part of the shell is hinged with a sealing cover through a hinge, the inner wall of the shell is fixedly covered with a heat preservation layer, the inner wall of the heat preservation layer is fixedly provided with a heating belt, a blood agar plate box is placed on the inner side of the heating belt, the inner wall of the shell is fixedly provided with a temperature sensor, and the heating belt and the temperature sensor are respectively electrically connected with the controller.
Preferably, the heating belt is a silica gel electric heating belt.
Preferably, the heat preservation layer is aerogel felt.
The beneficial effects of the utility model are as follows:
according to the utility model, the connecting steps and the buckling connection between the upper shell of the blood agar plate vessel and the lower shell of the blood agar plate vessel are arranged, so that the possibility that the upper shell of the blood agar plate vessel is loose due to loose buckling can be eliminated; the lower shell of the blood agar plate vessel and the upper shell of the blood agar plate vessel are made of transparent materials, so that the culture condition of an internal blood agar plate culture substrate can be directly observed, and the blood agar plate vessel can be directly inspected by a common optical microscope and is convenient to use; through setting up accuse temperature culture apparatus, can realize the temperature control culture function to blood agar plate culture substrate to whether it is qualified to the inspection, and then carries out subsequent use.
Drawings
FIG. 1 is a schematic diagram of a blood agar plate according to an embodiment of the present utility model;
FIG. 2 is a top view of the lower shell of the blood agar plate vessel according to the embodiment of the utility model;
FIG. 3 is a schematic diagram of a temperature-controlled culture apparatus according to an embodiment of the present utility model;
fig. 4 is a top view of the interior of the housing according to an embodiment of the present utility model.
Reference numerals: the blood agar plate vessel comprises a blood agar plate vessel lower shell 1, a blood agar plate vessel upper shell 2, a connecting step 3, a blood agar plate culture substrate 4, a partition plate 5, a buckle piece 6, a clamping block 7, a sealing cover 8, a shell 9, a buckle 10, a temperature sensor 11, a heat preservation layer 12, a heating belt 13, a blood agar plate box 14 and a controller 15.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-4, a blood agar plate comprises a blood agar plate vessel lower shell 1, a blood agar plate culture matrix 4, a blood agar plate vessel upper shell 2, a partition plate 5 and a temperature control culture device, wherein the blood agar plate culture matrix 4 is filled in the blood agar plate vessel lower shell 1, the partition plate 5 is integrally glued at the middle position of the inner side of the blood agar plate vessel lower shell 1, a connecting step 3 is arranged on the outer side of the blood agar plate vessel lower shell 1, the blood agar plate vessel upper shell 2 is buckled on the connecting step 3, the blood agar plate vessel upper shell 2 is also connected with the blood agar plate vessel lower shell 1 in a buckling manner, the blood agar plate vessel lower shell 1 and the blood agar plate vessel upper shell 2 are made of transparent materials, a blood agar plate box 14 is formed by the blood agar plate vessel lower shell 1, the blood agar plate culture matrix 4 and the blood agar plate vessel upper shell 2, and the blood agar plate box 14 is placed in the temperature control culture device.
In this example, the blood agar plate culture medium 4 is nutrient agar (Ph7.6) which is heated to be dissolved and cooled to 45-50 ℃, so that 5-10 ml of sterilized defibrinated sheep blood or rabbit blood is added into each 100ml of nutrient agar for sterilization, the nutrient agar plate culture medium is gently shaken up and immediately poured into the lower shell 1 of the blood agar plate vessel.
In this example, the blood agar plate culture medium 4 had the following composition: 10g of peptone, 3g of beef extract, 5g of sodium chloride, 15-20 g of agar, 50-100ml of sterile defibrinated sheep blood and 1000ml of distilled water, and the final Ph of the blood agar plate culture substrate 4 is 7.6.
In this embodiment, the blood agar plate vessel lower shell 1 and the blood agar plate vessel upper shell 2 are both made of polystyrene materials.
In the embodiment, the lower shell 1 and the upper shell 2 of the blood agar plate vessel are made of polystyrene materials, wherein the polystyrene materials have extremely high transparency, the light transmittance can reach more than 90 percent, the internal culture condition of the blood agar plate culture substrate 4 can be conveniently and directly observed, and the blood agar plate culture substrate can be directly inspected by a common optical microscope and is convenient to use.
In this embodiment, the side of the upper shell 2 of the blood agar plate vessel is hinged with a fastener 6 through a hinge seat, and the side of the lower shell 1 of the blood agar plate vessel is provided with a clamping block 7 matched with the fastener 6.
In the embodiment, the upper shell 2 of the blood agar plate vessel and the lower shell 1 of the blood agar plate vessel can be fixed by arranging the clamping piece 6 and the clamping block 7, so that the possibility that the upper shell 2 of the blood agar plate vessel is loose due to loose buckling can be eliminated, and the structural stability is improved.
In this embodiment, the blood agar plate cassette 14 has a cylindrical structure.
In the present embodiment, the blood agar plate cassette 14 may have a cylindrical structure or a rectangular parallelepiped structure, and is not limited thereto.
In the embodiment, the temperature control culture device comprises a shell 9 and a controller 15 arranged on the outer side wall of the shell 9, wherein the upper part of the shell 9 is hinged with a sealing cover 8 through a hinge, the inner wall of the shell 9 is fixedly covered with an insulating layer 12, the inner wall of the insulating layer 12 is fixedly provided with a heating belt 13, a blood agar plate box 14 is placed on the inner side of the heating belt 13, the inner wall of the shell 9 is also fixedly provided with a temperature sensor 11, and the heating belt 13 and the temperature sensor 11 are respectively electrically connected with the controller 15; the heating belt 13 is a silica gel electric heating belt 13; the insulation layer 12 is an aerogel blanket.
In this embodiment, the casing 9 and the cover 8 are hinged by a hinge, and are also connected by a buckle 10, so that the stability of the connection between the two is improved.
In this embodiment, through setting up heat preservation 12, can make casing 9 have the heat preservation effect, prevent the heat dissipation in the casing 9 to through setting up heating band 13 to placing the blood agar plate box 14 in its inside and heating, then implement the temperature in the monitoring casing 9 according to temperature sensor 11, and feed back to controller 15, controller 15 is according to the temperature threshold value of predetermineeing, and then control heating band 13 whether continue to work, in order to realize the temperature control cultivation effect.
In this embodiment, the step of culturing the blood agar plate culture medium 4 in a temperature-controlled manner is a prior art, including but not limited to a blood agar plate with reference to publication No. CN110819570A and a preparation method thereof, which indicates that the blood agar plate needs to be cultured at 30-35 ℃ for 24-36 hours for examination, and only after culturing, the blood agar plate without the growth of mixed bacteria can be identified as a qualified product for subsequent use.
Finally, it is noted that the above embodiments are only for illustrating the technical solution of the present utility model and not for limiting the same, and although the present utility model has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the technical solution of the present utility model, which is intended to be covered by the scope of the claims of the present utility model.
Claims (7)
1. The utility model provides a blood agar plate, includes blood agar plate ware inferior valve, blood agar plate culture substrate, blood agar plate ware epitheca, division board and accuse temperature culture apparatus, blood agar plate culture substrate fills the inside at blood agar plate ware inferior valve, division board integration glue joint in the inboard intermediate position of blood agar plate ware inferior valve, its characterized in that, the outside of blood agar plate ware inferior valve is equipped with the connection step, blood agar plate ware epitheca lock is on the connection step, blood agar plate ware epitheca still is connected with blood agar plate ware inferior valve buckle, blood agar plate ware inferior valve and blood agar plate ware epitheca all adopt transparent material to make, blood agar plate ware inferior valve, blood agar plate culture substrate and blood agar plate ware epitheca constitute blood agar plate box, blood agar plate box is placed in accuse temperature culture apparatus inside.
2. The blood agar plate according to claim 1, wherein the lower shell of the blood agar plate vessel and the upper shell of the blood agar plate vessel are made of polystyrene material.
3. The blood agar plate according to claim 1, wherein the side surface of the upper shell of the blood agar plate vessel is hinged with a clamping piece through a hinge seat, and a clamping block matched with the clamping piece is arranged on the side surface of the lower shell of the blood agar plate vessel.
4. The blood agar plate of claim 1, wherein the blood agar plate cassette has a cylindrical configuration.
5. The blood agar plate according to claim 1, wherein the temperature-controlled culture device comprises a shell and a controller arranged on the outer side wall of the shell, the upper portion of the shell is hinged with a sealing cover through a hinge, the inner wall of the shell is fixedly covered with a heat-insulating layer, a heating belt is fixedly arranged on the inner wall of the heat-insulating layer, a blood agar plate box is arranged on the inner side of the heating belt, a temperature sensor is fixedly arranged on the inner wall of the shell, and the heating belt and the temperature sensor are respectively electrically connected with the controller.
6. The blood agar plate of claim 5, wherein the heating belt is a silica gel electric heating belt.
7. The blood agar plate of claim 5, wherein the insulating layer is an aerogel blanket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320114702.7U CN219136769U (en) | 2023-01-19 | 2023-01-19 | Blood agar plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320114702.7U CN219136769U (en) | 2023-01-19 | 2023-01-19 | Blood agar plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219136769U true CN219136769U (en) | 2023-06-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320114702.7U Active CN219136769U (en) | 2023-01-19 | 2023-01-19 | Blood agar plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219136769U (en) |
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2023
- 2023-01-19 CN CN202320114702.7U patent/CN219136769U/en active Active
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