CN217757458U - Blood agar plate - Google Patents
Blood agar plate Download PDFInfo
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- CN217757458U CN217757458U CN202221312908.2U CN202221312908U CN217757458U CN 217757458 U CN217757458 U CN 217757458U CN 202221312908 U CN202221312908 U CN 202221312908U CN 217757458 U CN217757458 U CN 217757458U
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- blood agar
- agar plate
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- culture medium
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Abstract
The utility model provides a blood agar plate, which comprises a lower blood agar plate vessel shell, a blood agar plate culture matrix, an upper blood agar plate vessel shell, a partition plate and a magnifier, wherein the blood agar plate culture matrix is filled in the lower blood agar plate vessel shell; the upper shell of the blood agar plate vessel is buckled on the upper part of the lower shell of the blood agar plate vessel; the separation plate is integrally glued at the middle position of the lower part of the blood agar plate culture substrate; the magnifying glasses are respectively embedded at the left side and the right side of the inside of the blood agar plate culture substrate. The utility model divides the blood agar plate culture medium into two parts by penetrating the blood agar plate culture medium at the division plate, thereby observing the growth conditions at the two sides of the blood agar plate culture medium; under the action of the magnifier, the growth condition of microorganisms in the blood agar plate culture medium can be timely and comparatively observed by naked eyes.
Description
Technical Field
The utility model belongs to the technical field of the microbial cultivation, especially, relate to a blood agar flat plate.
Background
The culture medium is a nutrient medium prepared from different nutrient substances for the growth and reproduction of microorganisms, plants or animals (or tissues). Generally comprises carbohydrate, nitrogen-containing substances, inorganic salts (including trace elements), vitamins, water and the like. The culture medium is not only a basic substance for providing nutrition for the cells and promoting the proliferation of the cells, but also a living environment for the growth and the propagation of the cells. The culture medium has many kinds, and can be divided into natural culture medium, synthetic culture medium and semi-synthetic culture medium according to the source of the preparation raw material; can be divided into solid culture medium, liquid culture medium and semisolid culture medium according to physical state; according to the culture function, the culture medium can be divided into a basic culture medium, a selective culture medium, an enrichment culture medium, an identification culture medium and the like; according to the application range, the culture medium can be divided into a bacteria culture medium, an actinomycete culture medium, a yeast culture medium, a fungus culture medium and the like.
The media is typically tested and pH adjusted after preparation, and sterilized, typically by autoclaving and filtration. The culture medium is rich in nutrients and is easy to be polluted or deteriorated. It should not be placed for a long time after being prepared, and is best to be prepared and used at present
The blood plate culture medium, commonly named blood agar plate culture medium, is used for separating and culturing streptococcus and other fastidious bacteria and detecting hemolytic activity.
In addition, the Chinese patent publication No. CN110819570A is invented and created as a blood agar plate, which relates to the technical field of culture medium and contains various substances such as tryptone, soybean peptone and the like. However, the existing blood agar plates also have the problems of inconvenient separation and contrast culture in a vessel and inconvenient operation and use.
Therefore, it is necessary to develop a blood agar plate.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a blood agar plate to solve current blood agar plate and be not convenient for separate the contrast culture in the household utensils, operate and use inconvenient problem.
A blood agar plate comprises a lower blood agar plate vessel shell, a blood agar plate culture substrate, an upper blood agar plate vessel shell, a partition plate and a magnifier, wherein the blood agar plate culture substrate is filled in the lower blood agar plate vessel shell; the upper shell of the blood agar plate vessel is buckled on the upper part of the lower shell of the blood agar plate vessel; the separation plate is integrally glued at the middle position of the lower part of the blood agar plate culture substrate; the magnifying glasses are respectively embedded in the left side and the right side of the interior of the blood agar plate culture medium;
the blood agar plate culture medium is prepared by taking nutrient agar (ph 7.3 +/-0.2), heating to dissolve the nutrient agar, cooling to 45-50 ℃, adding sterilized defibered sheep blood or rabbit blood-ml into each ml of nutrient agar by sterilization, shaking up gently, and pouring into a lower shell of a blood agar plate vessel immediately;
the blood agar plate container lower shell, the blood agar plate culture medium and the blood agar plate container upper shell form a blood agar plate box, and the blood agar plate box is connected in the storage rack in a clamping mode.
Preferably, the final pH of the blood agar plate culture medium is 7.3 +/-0.2.
Preferably, the storage rack further comprises a supporting bottom plate, moisture-proof support legs, a clamping seat, a supporting suspension rod, a portable handle, a tool supporting plate and a disposable supporting box, wherein the moisture-proof support legs are respectively connected to four corners of the lower portion of the supporting bottom plate through screws; the clamping seat is glued at the middle position of the upper part of the supporting bottom plate; the supporting suspender is in threaded connection with the middle position of the left side of the upper part of the supporting base plate; the portable handle screw is connected to the upper end of the supporting suspender; the tool supporting plate is axially connected to the upper part of the right side of the supporting suspender; the disposable supporting box is clamped on the right side of the supporting suspender.
Preferably, the tool supporting plate is provided with an annular sliding slot hole in the middle of the inside, the left side of the inside of the sliding slot hole is slidably clamped with a dropper, and the right side of the inside of the sliding slot hole is slidably clamped with a coating rod.
Preferably, the clamping seat is a concave heat-insulating rubber seat; the lower shell of the blood agar plate vessel is clamped in the clamping seat.
Preferably, a tension spring is connected between the tool supporting plate and the upper part of the supporting hanger rod through a screw.
Preferably, the blood agar plate culture medium contains pancreatic digest of casein, pancreatic digest of heart, gastric digest of meat, yeast extract powder, soluble starch for providing carbon and nitrogen source, vitamins and growth factors; sheep blood is a good nutrient for the growth and reproduction of bacteria. The addition of blood to the basic culture medium at 45-50 deg.c can preserve some heat-labile growth factors in blood without damage to blood cells.
Preferably, the partition plate penetrates through the blood agar plate culture medium to divide the blood agar plate culture medium into two parts.
Compared with the prior art, the beneficial effects of the utility model are that:
the division plate penetrates through the blood agar plate culture substrate to divide the blood agar plate culture substrate into two parts, so that the growth conditions of two sides of the blood agar plate culture substrate are observed;
under the action of a magnifier, the growth condition of microorganisms in the blood agar plate culture medium can be timely compared and observed by naked eyes;
the use of frame is accomodate in the cooperation, can provide the convenience for the addition of fungus liquid material thing, and drip tube and coating rod take the convenience moreover, and the experiment of the blood agar plate culture base member of being convenient for is used, is convenient for carry the removal to blood agar plate culture base member moreover and uses, the use when the demonstration explanation of being convenient for.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic view of the top view structure of the lower casing of the blood agar plate vessel of the present invention.
Fig. 3 is a schematic bottom view of the upper shell of the blood agar plate vessel of the present invention.
Fig. 4 is a schematic structural diagram of an embodiment of the present invention.
Fig. 5 is a schematic top view of the tool support plate of the present invention.
In the figure:
1. a lower shell of a blood agar plate vessel; 2. blood agar plate culture medium; 3. blood agar plate ware upper shell; 4. a partition plate; 5. a magnifying glass; 6. a support base plate; 7. moisture-proof support legs; 8. a card holder; 9. a support boom; 10. a carrying handle; 11. a tool pallet; 111. a dropper; 112. coating rods; 113. a tension spring; 114. a sliding slot hole; 12. a disposable tray.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
example (b):
as shown in the attached drawings 1 to 5, the utility model provides a blood agar plate, which comprises a blood agar plate vessel lower shell 1, a blood agar plate culture substrate 2, a blood agar plate vessel upper shell 3, a division plate 4 and a magnifier 5, wherein the blood agar plate culture substrate 2 is filled in the blood agar plate vessel lower shell 1; the upper shell 3 of the blood agar plate vessel is buckled on the upper part of the lower shell 1 of the blood agar plate vessel; the division plate 4 is integrally glued at the middle position of the lower part of the blood agar plate culture substrate 2; the magnifier 5 is respectively embedded at the left side and the right side of the inside of the blood agar plate culture substrate 2;
the blood agar plate culture medium 2 is prepared by taking nutrient agar (ph 7.3 +/-0.2), heating to dissolve the nutrient agar, cooling to 45-50 ℃, adding 5-10 ml of sterilized defibered sheep blood or rabbit blood per 100ml of nutrient agar by sterilization, shaking up gently, and pouring into the lower shell 1 of a blood agar plate vessel immediately;
the casein costume composition of the blood agar plate culture medium 2 is as follows: 10g of pancreatin protein digest, 3g of pancreatin heart digest, 1g of corn starch, 5g of gastric enzyme digest, 5g of yeast extract powder, 5g of sodium chloride, 15g of agar, 50-100ml of aseptic defibrinated sheep blood and 1000ml of distilled water; the final pH7.3 + -0.2 of the blood agar plate culture medium 2.
The blood agar plate box is formed by a blood agar plate vessel lower shell 1, a blood agar plate culture matrix 2 and a blood agar plate vessel upper shell 3, and is connected in the storage rack in a clamping manner.
In the above embodiment, specifically, the storage rack further includes a support base plate 6, moisture-proof support legs 7, a clamping seat 8, a support suspension rod 9, a carrying handle 10, a tool supporting plate 11 and a disposable supporting box 12, wherein the moisture-proof support legs 7 are respectively screwed at four corners of the lower portion of the support base plate 6; the clamping seat 8 is glued at the middle position of the upper part of the supporting bottom plate 6; the supporting suspender 9 is in threaded connection with the middle position of the left side of the upper part of the supporting bottom plate 6; the portable handle 10 is connected with the upper end of the supporting suspender 9 through a screw; the tool supporting plate 11 is coupled at the upper position of the right side of the supporting suspender 9 by a shaft; the disposable supporting box 12 is clamped on the right side of the supporting suspension rod 9.
In the above embodiment, specifically, an annular slide slot hole 114 is formed in the middle position inside the tool supporting plate 11, a dropper 111 is slidably engaged at the left side inside the slide slot hole 114, and a coating rod 112 is slidably engaged at the right side inside the slide slot hole 114.
In the above embodiment, specifically, the clamping seat 8 specifically adopts a concave thermal insulation rubber seat; the lower casing 1 of the blood agar plate vessel is clamped in the clamping seat 8.
In the above embodiment, in particular, a tension spring 113 is connected between the tool supporting plate 11 and the upper portion of the support suspension rod 9 by a screw.
In the above embodiment, the partition plate 4 is configured to divide the blood agar plate culture medium 2 into two parts by penetrating the blood agar plate culture medium 2.
In the above embodiment, specifically, the blood agar plate culture medium 2 contains tryptic casein digest, pancreatic cardiology digest, pepsin digest, yeast extract powder, soluble starch to provide a carbon-nitrogen source, vitamins and growth factors; sheep blood is a good nutrient for the growth and reproduction of bacteria. The addition of blood to the basic culture medium at 45-50 deg.c can preserve some heat-labile growth factors in blood without damage to blood cells.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described above, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. The blood agar plate is characterized by comprising a blood agar plate vessel lower shell (1), a blood agar plate culture substrate (2), a blood agar plate vessel upper shell (3), a partition plate (4) and a magnifying glass (5), wherein the blood agar plate culture substrate (2) is filled in the blood agar plate vessel lower shell (1); the upper shell (3) of the blood agar plate vessel is buckled on the upper part of the lower shell (1) of the blood agar plate vessel; the partition plate (4) is integrally glued at the middle position of the lower part of the blood agar plate culture substrate (2); the magnifying glasses (5) are respectively embedded in the left side and the right side of the interior of the blood agar plate culture matrix (2);
the blood agar plate container comprises a blood agar plate container lower shell (1), a blood agar plate culture medium (2) and a blood agar plate container upper shell (3), which form a blood agar plate box, and the blood agar plate box is clamped in the storage frame.
2. The blood agar plate according to claim 1, wherein the storage rack further comprises a support bottom plate (6), moisture-proof legs (7), a clamping seat (8), a support hanging rod (9), a handle (10), a tool supporting plate (11) and a disposable supporting box (12), wherein the moisture-proof legs (7) are respectively screwed at the four corners of the lower part of the support bottom plate (6); the clamping seat (8) is glued at the middle position of the upper part of the supporting bottom plate (6); the supporting hanger rod (9) is in threaded connection with the middle position of the left side of the upper part of the supporting bottom plate (6); the portable handle (10) is connected to the upper end of the supporting suspender (9) through a screw; the tool supporting plate (11) is coupled at the upper position of the right side of the supporting suspender (9) by a shaft; the disposable supporting box (12) is clamped on the right side of the supporting suspender (9).
3. The blood agar plate according to claim 2, wherein the tool supporting plate (11) is provided with a ring-shaped sliding slot hole (114) at the middle position inside, the dropper (111) is slidably clamped at the left side inside the sliding slot hole (114), and the coating rod (112) is slidably clamped at the right side inside the sliding slot hole (114).
4. Blood agar plate according to claim 2, characterized in that the holder (8) is a concave thermal rubber holder; the blood agar plate vessel lower shell (1) is clamped in the clamping seat (8).
5. Blood agar plate according to claim 2, characterized in that a tension spring (113) is screwed between the tool carrier (11) and the upper part of the support boom (9).
6. Blood agar plate according to claim 1, characterized in that the partition plate (4) divides the blood agar plate culture medium (2) in two across the blood agar plate culture medium (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221312908.2U CN217757458U (en) | 2022-05-30 | 2022-05-30 | Blood agar plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221312908.2U CN217757458U (en) | 2022-05-30 | 2022-05-30 | Blood agar plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217757458U true CN217757458U (en) | 2022-11-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221312908.2U Active CN217757458U (en) | 2022-05-30 | 2022-05-30 | Blood agar plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217757458U (en) |
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2022
- 2022-05-30 CN CN202221312908.2U patent/CN217757458U/en active Active
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