CN219117467U - Automatic liquid changing device for cell culture - Google Patents
Automatic liquid changing device for cell culture Download PDFInfo
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- CN219117467U CN219117467U CN202223610102.4U CN202223610102U CN219117467U CN 219117467 U CN219117467 U CN 219117467U CN 202223610102 U CN202223610102 U CN 202223610102U CN 219117467 U CN219117467 U CN 219117467U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
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Abstract
The utility model relates to the field of biotechnology, in particular to an automatic liquid changing device for cell culture, which comprises a body, wherein a plurality of culture liquid storage tanks are arranged above the inside of the body, and a waste liquid storage tank is arranged below the inside of the body; the below of culture solution storage tank is equipped with the multilayer and puts the thing board, is equipped with cell culture household utensils on every layer of thing board, and the top of every cell culture household utensils is equipped with buffer unit, and the inlet of every buffer unit communicates a plurality of culture solution storage tanks respectively, and the liquid outlet of every buffer unit communicates the cell culture household utensils of below, and the waste liquid export of cell culture household utensils communicates the waste liquid storage tank. The automatic liquid changing device with the structure has reasonable design, is convenient for changing the culture liquid at different stages required by the growth of the cells, and can also avoid the damage to the cells caused by the impact force when the culture liquid is added.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to an automatic liquid exchange device for cell culture.
Background
In cell biology research and life science research, cell culture is an essential link; cell culture refers to adding cells and culture fluid into a culture dish, sealing and preserving the environment temperature, humidity and oxygen content required by the controlled cells, and replacing the culture fluid at regular time and quantity. At present, when the culture solution is replaced in cell culture, usually need the experimenter take out the culture dish from the culture system many times, add new culture solution after absorbing useless culture solution, put into the required environment of cell culture with the culture dish again, above-mentioned operation has increased the risk that the cell was polluted, when the experimenter removes the waste liquid and adds new culture solution in addition, because the operation method is different, causes certain influence to the cell, and the whole in-process of cell culture needs to add the culture solution many times, complex operation moreover. Although an automatic liquid-changing device exists at present, when fresh culture liquid is added, the impact force generated when the culture liquid flows can damage cells; in addition, in the cell culture process, culture solutions with different components may need to be supplemented, and the existing automatic liquid exchange device does not have the functions. Therefore, in order to solve the above-mentioned problems, it is necessary to develop an automatic liquid changing device for cell culture.
Disclosure of Invention
The technical problems to be solved by the utility model are as follows: aiming at the defects of the prior art, the automatic liquid exchange device for cell culture is provided, and the liquid exchange device can avoid damage to cells caused by adding culture liquid, and simultaneously, can conveniently add a plurality of different culture liquids.
In order to solve the technical problems, the technical scheme of the utility model is as follows:
the automatic liquid exchange device for cell culture comprises a body, wherein a plurality of culture liquid storage tanks are arranged above the inside of the body, and a waste liquid storage tank is arranged below the inside of the body; the cell culture device comprises a culture solution storage tank, and is characterized in that a plurality of storage plates are arranged below the culture solution storage tank, cell culture vessels are arranged on each storage plate, buffer components are arranged above each cell culture vessel, a liquid inlet of each buffer component is respectively communicated with a plurality of culture solution storage tanks, a liquid outlet of each buffer component is communicated with the cell culture vessel below, and a liquid outlet of each cell culture vessel is communicated with the liquid storage tank.
As an improved technical scheme, the plurality of culture solution tanks comprise a first culture solution tank, a second culture solution tank, a third culture solution tank and a fourth culture solution tank.
As an improved technical scheme, the buffer component comprises an overflow groove, a collecting groove is arranged below the overflow groove, and a plurality of liquid distribution pipes communicated with the cell culture vessel are arranged below the collecting groove.
As an improved technical scheme, the cell culture vessel comprises a vessel body and a vessel cover, wherein a waste liquid outlet is formed in one side of the vessel body, and a filter screen is arranged at the waste liquid outlet; be equipped with a plurality of feed liquor mouthfuls that are linked together with the cloth liquid pipe on the dish lid, the below of feed liquor mouthful be equipped with the cloth liquid structure that the feed liquor mouthful is linked together, cloth liquid structure includes the body, be equipped with a plurality of stock solution district that are linked together with the feed liquor mouth on the body, the outward flange of body is equipped with the breach, form the passageway between breach and the inner wall of dish lid, just be equipped with a plurality of liquid holes on the outer wall of stock solution district.
As an improved technical scheme, a plurality of culture solution storage tanks are communicated with the cell culture vessel through a cell solution conveying pipeline and a conveying pump, and a valve on the cell solution conveying pipeline and the conveying pump are respectively and electrically connected with a controller.
As an improved technical scheme, the waste liquid outlet is communicated with the waste liquid storage tank through a waste liquid pipeline, and a valve on the waste liquid pipeline is electrically connected with the controller.
As an improved technical scheme, a temperature sensor is arranged in the body, a jacket is arranged outside the body, and the temperature sensor is electrically connected with a controller.
After the technical scheme is adopted, the utility model has the beneficial effects that:
the automatic liquid exchange device for cell culture comprises a body, wherein a plurality of culture liquid storage tanks are arranged above the inside of the body, and a waste liquid storage tank is arranged below the inside of the body; the below of culture solution storage tank is equipped with the multilayer and puts the thing board, is equipped with cell culture household utensils on every layer of thing board, and the top of every cell culture household utensils is equipped with buffer unit, and the inlet of every buffer unit communicates a plurality of culture solution storage tanks respectively, and the liquid outlet of every buffer unit communicates the cell culture household utensils of below, and the waste liquid export of cell culture household utensils communicates the waste liquid storage tank. And adding the prepared culture solution into a culture solution storage tank, enabling the culture solution to enter a buffer component along a conveying pipeline under the action of a conveying pump, closing the conveying pump, enabling the culture solution in the buffer component to enter a cell culture vessel again, and enabling waste liquid to enter the waste solution storage tank along with a waste liquid pipeline after being replaced. According to the automatic liquid changing device with the structure, different culture liquids are added at different stages according to the requirement of cell growth, so that the requirement of cell growth is met. Through setting up buffer unit, reduced the velocity of flow of culture solution, avoided the too fast impact force that produces of culture solution velocity of flow to cause the damage to the cell. The automatic liquid changing device with the structure has reasonable design, is convenient for changing the culture liquid at different stages required by the growth of the cells, and can also avoid the damage to the cells caused by the impact force when the culture liquid is added.
Since the plurality of culture solution tanks includes the first culture solution tank, the second culture solution tank, the third culture solution tank, and the fourth culture solution tank. By arranging 4 cell liquid storage tanks, cell culture solutions with different components are conveniently supplied to cells, and the requirement of cell growth is met.
Because the buffer part comprises an overflow groove, a collecting groove is arranged below the overflow groove, and a plurality of liquid distribution pipes communicated with the cell culture vessel are arranged below the collecting groove. The culture solution in the culture solution storage tank enters the overflow groove along the pipeline under the action of the delivery pump, flows to the collecting groove below through the overflow eave, and then enters the cell culture vessel through the liquid distribution pipe. The cell liquid directly gets into the inside of cell culture household utensils under the effect of delivery pump, and the impact force of cell liquid causes the damage to the cell, through setting up buffer unit cell liquid and get into after the buffer unit, close the delivery pump, cell liquid gets into the inside of cell culture household utensils under the effect of gravity, the buffer unit of above-mentioned structure, greatly reduced the velocity of flow of culture liquid, reduced the damage to the cell.
The cell culture vessel comprises a vessel body and a vessel cover, wherein a waste liquid outlet is formed in one side of the vessel body, and a filter screen is arranged at the waste liquid outlet; be equipped with a plurality of feed liquor mouthfuls that are linked together with the cloth liquid pipe on the dish lid, the below of feed liquor mouthful is equipped with the cloth liquid structure that is linked together with the feed liquor mouth each other, and cloth liquid structure includes the body, is equipped with the stock solution district that is linked together with the feed liquor mouth on the body, and the outward flange of body is equipped with a plurality of breach, forms the passageway between the inner wall of breach and dish lid, and is equipped with a plurality of liquid outlet holes on the outer wall of stock solution district. The culture solution in the liquid distribution pipe enters the liquid storage area of the liquid distribution structure along the liquid inlet, passes through the liquid outlet hole of the liquid storage area and flows into the vessel along the channel. The waste liquid is discharged from the waste liquid outlet into the waste liquid storage tank. The cell culture vessel with the structure is reasonable in design, and the liquid distribution structure is arranged, so that the flow velocity of cell liquid is reduced, and the damage to cells is avoided.
Because the plurality of culture solution storage tanks are communicated with the cell culture vessel through the cell solution conveying pipeline and the conveying pump, the valves on the cell solution conveying pipeline and the conveying pump are respectively and electrically connected with the controller. The cell culture liquid with different components is conveniently conveyed into a plurality of cell culture vessels through the conveying pump, and the valve is controlled by the controller, so that automatic operation is realized.
Because the waste liquid outlet is communicated with the waste liquid storage tank through the waste liquid pipeline, a valve on the waste liquid pipeline is electrically connected with the controller. The valve on the waste liquid pipeline is controlled by the controller, so that the operation is convenient.
Because the inside of the body is provided with the temperature sensor, the outside of the body is provided with the jacket, and the temperature sensor is electrically connected with the controller. When the cell culture fluid is replaced, the temperature of the surrounding environment is considered, the temperature inside the body can be controlled through the temperature sensor, and when heating is needed, the temperature required by the growth of the cultured cells is provided by using the heating medium of the jacket.
Drawings
FIG. 1 is a schematic diagram of an automatic liquid exchange device for cell culture according to the present utility model;
FIG. 2 is a schematic diagram of the liquid distribution structure in FIG. 1;
FIG. 3 is a schematic diagram of the structure of the cell culture vessel of FIG. 1;
the device comprises a 1-body, a 2-culture solution storage tank, a 3-waste solution storage tank, a 4-storage plate, a 5-cell culture vessel, a 50-vessel body, a 500-waste solution outlet, a 501-filter screen, a 51-vessel cover, a 510-liquid inlet, a 6-buffer component, a 60-overflow tank, a 61-collecting tank, a 62-liquid distribution pipe, a 7-liquid distribution structure, a 70-liquid storage area, a 71-notch, an 8-waste solution pipeline, a 9-controller, a 10-temperature sensor, an 11-jacket and a 12-conveying pump.
Detailed Description
The present utility model will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.
An automatic liquid exchange device for cell culture, as shown in fig. 1-3, comprises a body 1, wherein a plurality of culture liquid storage tanks 2 are arranged above the inner part of the body 1, and a waste liquid storage tank 3 is arranged below the inner part of the body 1; the below of culture solution storage tank 2 is equipped with multilayer and puts thing board 4, is equipped with cell culture dish 5 on every layer of thing board 4, and the top of every cell culture dish 5 is equipped with buffer unit 6, and buffer unit 6 includes overflow launder 60, and the below of overflow launder 60 is equipped with collecting vat 61, and the below of collecting vat 61 is equipped with a plurality of cloth liquid pipes 62 that are linked together with cell culture dish 5. The cell culture vessel 5 comprises a vessel body 50 and a vessel cover 51, wherein a waste liquid outlet 500 is arranged on one side of the vessel body 50, a filter screen 501 is arranged at the waste liquid outlet 500, and the waste liquid outlet 500 is communicated with the waste liquid storage tank 3; be equipped with a plurality of feed liquor mouthfuls 510 that are linked together with cloth liquid pipe 62 on the dish lid 51, the below of feed liquor mouthfuls 510 is equipped with cloth liquid structure 7 that is linked together with feed liquor mouthfuls 510, cloth liquid structure 7 includes the body (both sides are linked firmly with the inner wall card of dish lid), be equipped with the stock solution district 70 that is linked together with the feed liquor mouthfuls on the body, the outward flange of body is equipped with a plurality of breach 71, form the passageway between breach 71 and the inner wall of dish lid 51, and be equipped with a plurality of drain holes on the outer wall of stock solution district 71.
The culture solution prepared is added into the culture solution storage tank, the culture solution enters the overflow groove of the buffer component along the conveying pipeline under the action of the conveying pump, the conveying pump is closed, the culture solution flows to the collecting groove below under the action of gravity through the overflow eave, then the culture solution enters the liquid distribution structure of the dish cover through the liquid distribution pipe, enters the liquid storage area, passes through the liquid outlet hole, flows into the dish body along the channel, and after the waste solution is replaced, the waste solution enters the inside of the waste solution storage tank along the waste solution pipeline. According to the automatic liquid changing device with the structure, different culture liquids are added at different stages according to the requirement of cell growth, so that the requirement of cell growth is met. Through setting up buffer unit, reduced the velocity of flow of culture solution, avoided the too fast impact force that produces of culture solution velocity of flow to cause the damage to the cell. The automatic liquid changing device with the structure has reasonable design, is convenient for changing the culture liquid at different stages required by the growth of the cells, and can also avoid the damage to the cells caused by the impact force when the culture liquid is added.
Wherein the plurality of culture medium tanks 2 comprises a first culture medium tank 20, a second culture medium tank 21, a third culture medium tank 22 and a fourth culture medium tank 23. By arranging 4 cell liquid storage tanks, cell culture solutions with different components are conveniently supplied to cells, and the requirement of cell growth is met.
Wherein, a plurality of culture solution storage tanks are communicated with the cell culture vessel through a cell solution conveying pipeline and a conveying pump, and a valve and the conveying pump on the cell solution conveying pipeline are respectively and electrically connected with a controller. The cell culture liquid with different components is conveniently conveyed into a plurality of cell culture vessels through the conveying pump, and the valve is controlled by the controller, so that automatic operation is realized.
Wherein the waste liquid outlet 500 is communicated with the waste liquid storage tank 3 through a waste liquid pipeline 8, and a valve on the waste liquid pipeline is electrically connected with the controller 9. The valve on the waste liquid pipeline is controlled by the controller, and the waste liquid is discharged from the outlet of the waste liquid storage tank.
Wherein the inside of the body 1 is provided with a temperature sensor 10, the outside of the body 1 is provided with a jacket 11, and the temperature sensor 10 is electrically connected with the controller 9. When the cell culture fluid is replaced, the temperature of the surrounding environment is considered, the temperature inside the body can be controlled through the temperature sensor, and when heating is needed, the temperature required by the growth of the cultured cells is provided by using the heating medium of the jacket.
The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the utility model.
Claims (7)
1. An automatic liquid changing device for cell culture, which is characterized in that: the culture medium storage device comprises a body, wherein a plurality of culture medium storage tanks are arranged above the inside of the body, and a waste liquid storage tank is arranged below the inside of the body; the cell culture device comprises a culture solution storage tank, and is characterized in that a plurality of storage plates are arranged below the culture solution storage tank, cell culture vessels are arranged on each storage plate, buffer components are arranged above each cell culture vessel, a liquid inlet of each buffer component is respectively communicated with a plurality of culture solution storage tanks, a liquid outlet of each buffer component is communicated with the cell culture vessel below, and a liquid outlet of each cell culture vessel is communicated with the liquid storage tank.
2. The automatic liquid changing device for cell culture according to claim 1, wherein: the plurality of culture solution storage tanks comprise a first culture solution storage tank, a second culture solution storage tank, a third culture solution storage tank and a fourth culture solution storage tank.
3. The automatic liquid changing device for cell culture according to claim 1, wherein: the buffer component comprises an overflow groove, a collecting groove is arranged below the overflow groove, and a plurality of liquid distribution pipes communicated with the cell culture vessel are arranged below the collecting groove.
4. An automatic liquid changing device for cell culture according to claim 3, wherein: the cell culture vessel comprises a vessel body and a vessel cover, wherein a waste liquid outlet is formed in one side of the vessel body, and a filter screen is arranged at the waste liquid outlet; be equipped with a plurality of feed liquor mouthfuls that are linked together with the cloth liquid pipe on the dish lid, the below of feed liquor mouthful be equipped with the cloth liquid structure that the feed liquor mouthful is linked together, cloth liquid structure includes the body, be equipped with a plurality of stock solution district that are linked together with the feed liquor mouth on the body, the outward flange of body is equipped with a plurality of breach, form the passageway between breach and the inner wall of dish lid, just be equipped with a plurality of liquid holes on the outer wall of stock solution district.
5. The automatic liquid changing device for cell culture according to claim 1, wherein: the plurality of culture solution storage tanks are communicated with the cell culture vessel through a cell solution conveying pipeline and a conveying pump, and a valve on the cell solution conveying pipeline and the conveying pump are respectively and electrically connected with a controller.
6. The automatic liquid changing device for cell culture according to claim 1, wherein: the waste liquid outlet is communicated with the waste liquid storage tank through a waste liquid pipeline, and a valve on the waste liquid pipeline is electrically connected with the controller.
7. The automatic liquid changing device for cell culture according to claim 1, wherein: the inside of body is equipped with temperature sensor, the outside of body is equipped with the clamp cover, temperature sensor electricity connection director.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223610102.4U CN219117467U (en) | 2022-12-30 | 2022-12-30 | Automatic liquid changing device for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223610102.4U CN219117467U (en) | 2022-12-30 | 2022-12-30 | Automatic liquid changing device for cell culture |
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| Publication Number | Publication Date |
|---|---|
| CN219117467U true CN219117467U (en) | 2023-06-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223610102.4U Active CN219117467U (en) | 2022-12-30 | 2022-12-30 | Automatic liquid changing device for cell culture |
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| CN (1) | CN219117467U (en) |
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2022
- 2022-12-30 CN CN202223610102.4U patent/CN219117467U/en active Active
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