CN219104736U - Homogeneous chemiluminescent detection system - Google Patents

Abstract

The application provides a homogeneous phase chemiluminescence detecting system, which relates to the technical field of homogeneous phase chemiluminescence detection. The centrifugal device of the homogeneous chemiluminescence detection system is provided with a working position for detection in advance, and the centrifugal device is provided with a plurality of stations for installing reagent cards and clamping points corresponding to the stations one by one. The driving device is connected with the centrifugal device in a driving way so as to enable one station of the centrifugal device to rotate to a working position. The elastic piece of the positioning device is arranged on the positioning pin, and the driving piece drives the positioning pin to be separated from the clamping point; when the station is not rotated to the working position, the elastic piece enables the positioning pin to be abutted against the centrifugal device, and when one station is rotated to the working position, the elastic piece enables the positioning pin to be clamped in the clamping point. The system does not need to use a servo motor to accurately position the rotation condition of the centrifugal device, can use a common motor to accurately position the rotation condition of the centrifugal device in cooperation with the positioning device, and can reduce the cost of the homogeneous phase chemiluminescence detection system.

Description

Homogeneous chemiluminescent detection system

Technical Field

The application relates to the technical field of homogeneous chemiluminescent detection, in particular to a homogeneous chemiluminescent detection system.

Background

Chemiluminescent immunoassay (chemiluminescence immunoassay, CLIA) combines a chemiluminescent assay technique with high sensitivity with a highly specific immune reaction, and is used for various antigens, haptens, small molecules, antibodies, drugs, and other analytical techniques. The separation and washing steps are classified into a heterogeneous chemiluminescence method and a homogeneous chemiluminescence method according to the presence or absence of the separation and washing steps.

The heterogeneous chemiluminescent immunoassay method mainly comprises electrochemiluminescence, enzymatic chemiluminescence and the like. It is necessary to separate and remove unreacted free label and determine the signal intensity of the bound label. In general, a solid-phase adsorption separation mode, i.e., magnetic microspheres are used as solid-phase materials, so that the bound state markers are adsorbed on the surfaces of the microspheres, and the free state markers are distributed in a liquid phase, and then the free markers are removed by washing the microspheres. And the homogeneous chemiluminescent immunoassay method does not need separation and washing.

The detection principle of the current homogeneous chemiluminescent detection system is as follows: the sample is transferred by a pipette, mixed with the reagent, and then detected. The need for a robotic arm and transfer during sample transfer results in a large volume homogeneous chemiluminescent detection system.

Disclosure of Invention

Therefore, the inventor designs a homogeneous chemiluminescent detection system, the detection principle of which is as follows: when the detection is needed, the sample to be detected is placed in the reagent card, the reagent card is placed in a centrifugal device of a homogeneous chemiluminescence detection system, high-speed centrifugation is carried out at a speed of thousands of revolutions per minute, the sample to be detected and the nuclear reagent are mixed in the reagent card to obtain a substance to be detected, and the substance to be detected is incubated for a period of time so as to detect the substance to be detected in the incubation.

The inventor continues to research and find that in order to improve the detection efficiency, in general, the aligned position of the detection device is called a working position, the centrifugal device is provided with a plurality of stations, each station is provided with a reagent card, when the first station rotates to the working position, the detection device can detect the luminous signal intensity of a substance to be detected in the reagent card on the first station, then the centrifugal device is controlled to slowly rotate through the servo motor, the reagent card on the second station precisely rotates to the working position, and then the detection device detects the substance to be detected in the reagent card on the second station. However, the centrifugal device is controlled to precisely rotate by the servo motor, so that the requirement on the servo motor is high, and the cost of the homogeneous chemiluminescent detection system is high.

An object of the embodiment of the application is to provide a homogeneous chemiluminescence detection system, and accurate positioning between reagent cards and detection devices on different stations can be achieved by using a common motor.

The embodiment of the application provides a homogeneous phase chemiluminescence detection system, which comprises a bracket, a centrifugal device, a driving device and a positioning device. The centrifugal device is rotatably arranged on the support, the centrifugal device is provided with a working position for detection in advance, and the centrifugal device is provided with a plurality of stations for installing reagent cards and clamping points corresponding to the stations one by one. The driving device is connected with the centrifugal device in a driving way so as to enable one station of the centrifugal device to rotate to a working position. The positioning device comprises a driving piece, an elastic piece and a positioning pin, wherein the elastic piece is arranged on the positioning pin, and the driving piece is matched with the elastic piece, so that the positioning pin has a first state of being separated from a clamping point, a second state of being abutted against the centrifugal device and a third state of being clamped in the clamping point.

In the implementation process, the centrifugal device is provided with a plurality of stations, each station is provided with a reagent card, one position of the centrifugal device is defined as a working position, when the locating pin of the locating device is clamped on one clamping point, the detection device corresponds to the preset station, the reagent card at the working position can be detected, after the detection device detects that the locating pin is separated from the clamping point, the driving device drives the centrifugal device to rotate, at the moment, the locating pin is propped against the centrifugal device, when one of the stations rotates to the working position, the locating pin is clamped on the clamping point, the driving device can stop driving the centrifugal device to rotate through the clamping of the locating pin, and at the moment, a new reagent card is placed at the working position. The system does not need to use a servo motor to accurately position the rotation condition of the centrifugal device, can use a common motor to accurately position the rotation condition of the centrifugal device in cooperation with the positioning device, and can reduce the cost of the homogeneous phase chemiluminescence detection system.

In one possible embodiment, the driving member is deactivated before the station is rotated, and the elastic member brings the positioning pin into the first state; when the work stations are not rotated to the working positions, the driving piece drives the positioning pins to be in the second state, and when one work station is rotated to the working positions, the driving piece drives the positioning pins to be in the third state.

In the implementation process, when the positioning pin is required to be clamped, the driving piece drives the positioning pin to move; when the locating pin is reset, the driving piece does not work and is driven by the elastic piece, so that the service life of the locating mechanism is longer, and the requirement on the elastic piece is not high.

In one possible embodiment, the positioning device further comprises a sleeve, the elastic piece is a spiral spring, the spiral spring is sleeved outside the positioning pin, the upper section of the spiral spring is arranged in the sleeve, the lower end of the spiral spring is fixed on the positioning pin, and the upper end of the spiral spring is fixed on the driving piece.

In the realization process, the sleeve is arranged to facilitate the installation of the locating pin and the spiral spring, and simultaneously, when the driving piece does not work, the lower end of the locating pin is conveniently moved downwards through the rebound effect of the spiral spring, so that the locating pin is separated from the clamping point. In one possible embodiment, the driving member is an electromagnet, the electromagnet is disposed at the lower end of the positioning pin, and a magnetic core of the electromagnet is in contact with the lower end of the positioning pin, and when the electromagnet is energized, the magnetic core of the electromagnet moves upward and moves upward in synchronization with the positioning pin; when the electromagnet is powered off, the spiral spring rebounds to enable the locating pin to be separated from the clamping point.

In the implementation process, the magnetic core of the electromagnet is in a contact state with the positioning pin, and the magnetic core of the electromagnet directly acts on the positioning pin to control the positioning pin to move, so that the material of the positioning pin is not limited to be a magnetic substance; meanwhile, the magnetic core is used for directly controlling the positioning pin to be clamped at the clamping point, the effect of the magnetic core is relatively large, the magnetic core is easier to realize, the requirement on the coil spring is reduced, and the cost of the electromagnet is low, so that the equipment cost of the homogeneous phase chemiluminescence detection system is reduced.

In one possible embodiment, the driving member is a linear driving member, the linear driving member is arranged at the lower end of the positioning pin, and the linear driving member drives the elastic member and the positioning pin to move downwards so as to separate the positioning pin from the clamping point; or the linear driving piece drives the elastic piece and the locating pin to move, and the elastic piece rebounds, so that the upper end of the locating pin is abutted or clamped with the centrifugal device at the clamping point.

In the implementation process, the linear driving piece and the elastic piece are arranged, so that the lifting or the descending of the positioning pin is conveniently controlled, and the clamping position of the positioning pin is conveniently adjusted.

In one possible embodiment, the centrifugal device comprises a centrifugal disc, a heating film and a circuit board which are sequentially arranged from top to bottom, wherein a plurality of stations are arranged on the centrifugal disc, a plurality of clamping points are arranged on the circuit board, and the positioning pin is selectively clamped at one clamping point or is abutted against the circuit board.

In the implementation process, the reagent card is borne by the centrifugal disc and is clamped on different clamping points of the circuit board through the locating pin, so that the locating pin is clamped more conveniently, the clamping position of the locating pin is adjusted conveniently, and the clamping of the locating pin can not influence the installation of the reagent card.

In one possible implementation mode, the edges of the circuit board are provided with notches corresponding to the stations one by one, and the positioning pins are alternatively clamped in one notch; or the lower surface of the circuit board is provided with clamping holes corresponding to the stations one by one, and the positioning pin is selectively clamped in one clamping hole.

In the implementation process, the locating pin is clamped through the notch or the clamping hole, so that the locating pin can be clamped conveniently, and the locating pin is easier to separate from the clamping point, so that the locating pin can be positioned accurately.

In one possible embodiment, the driving device further comprises a zero position photoelectric device, wherein the zero position photoelectric device is arranged on the driving piece and is used for detecting whether the positioning pin is separated from the clamping point.

In the implementation process, the zero position photoelectric energy can judge the position of the locating pin so as to determine whether the driving device can drive the centrifugal device to rotate or not, and unnecessary limitation of the locating pin on rotation of the centrifugal device is avoided.

In one possible embodiment, the homogeneous chemiluminescent detection system further comprises a detection device for collecting and processing the luminescent signal intensity of the substance to be detected in the reagent card at the working location.

In the implementation process, through the arrangement of the detection device, the luminous signal intensity of the substance to be detected in the reagent card at the working position can be collected and processed so as to carry out homogeneous chemiluminescence analysis.

In one possible embodiment, the homogeneous chemiluminescent detection system further comprises a base plate and a housing, the base plate and housing forming a detection chamber, the support, the centrifugation device, the detection device, the drive device and the positioning device being disposed in the detection chamber.

In the implementation process, the main components in the homogeneous chemiluminescence detection system can be arranged in the detection chamber, so that the influence of external factors on the detection result is avoided, and the detection result can be more accurate.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered limiting the scope, and that other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a homogeneous chemiluminescent detection system according to an embodiment of the present application;

FIG. 2 is a schematic diagram of a first internal structure of a homogeneous chemiluminescent detection system according to an embodiment of the present application;

FIG. 3 is a schematic diagram of a second internal structure of the homogeneous chemiluminescent detection system according to an embodiment of the present application;

FIG. 4 is a schematic diagram of a third internal structure of a homogeneous chemiluminescent detection system according to an embodiment of the present application;

FIG. 5 is an exploded view of a centrifuge device in a homogeneous chemiluminescent detection system provided in an embodiment of the present application;

FIG. 6 is a schematic structural diagram of a positioning device in a homogeneous chemiluminescent detection system according to an embodiment of the present application;

FIG. 7 is a cross-sectional view of a positioning device in a homogeneous chemiluminescent detection system according to an embodiment of the present application.

Icon: 10-a centrifugal device; 20-positioning means; 30-detecting means; 40-a bottom plate; 50-a housing; 60-reagent card; 70-driving means; 80-control means; 90-brackets;

111-centrifuge disk; 112-working position; 113-station; 121-a circuit board; 122-heating the film; 123-stuck point; 1231-gap; 220-locating pins; 211-a driving member; 212-an elastic member; 213-coil springs; 214-an electromagnet; 2141-a magnetic core; 216-sleeve; 217-a fixed seat; 218-null photovoltaic.

Detailed Description

For the purposes of making the objects, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, which are generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, as provided in the accompanying drawings, is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments herein without making any inventive effort, are intended to be within the scope of the present application.

It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.

In the description of the present application, it should be noted that, the azimuth or positional relationship indicated by the terms "center", "upper", "lower", "inner", "outer", etc. are based on the azimuth or positional relationship shown in the drawings, or the azimuth or positional relationship that the product of the application is conventionally put in use, are merely for convenience of describing the present application and simplifying the description, and do not indicate or imply that the apparatus or element referred to must have a specific azimuth, be configured and operated in a specific azimuth, and therefore should not be construed as limiting the present application. Furthermore, the terms "first," "second," and the like, are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.

In the description of the present application, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; either mechanically or electrically. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art in a specific context.

FIG. 1 is a schematic diagram of a homogeneous chemiluminescent detection system according to an embodiment of the present application; FIG. 2 is a schematic diagram of a first internal structure of a homogeneous chemiluminescent detection system according to an embodiment of the present application; FIG. 3 is a schematic diagram of a second internal structure of the homogeneous chemiluminescent detection system according to an embodiment of the present application; fig. 4 is a schematic diagram of a third internal structure of the homogeneous chemiluminescent detection system according to the embodiment of the present application, referring to fig. 1 to 4, the homogeneous chemiluminescent detection system includes a support 90, a centrifugal device 10, a positioning device 20, a detection device 30 and a driving device 70.

In this application, set up reagent card 60 on centrifugal device 10, hold in reagent card 60 and detect sample and reagent, sample and reagent are adorned respectively in different cavities, open the valve on the reagent card 60 during the use, drive centrifugal device 10 high-speed centrifugation through drive arrangement 70, make the liquid in the reagent card 60 flow along the runner, mix together sample and reagent (waiting to detect the material) and flow in a printing opacity detection zone. If the sample is whole blood (blood-containing cells), the blood cells need to be disrupted and separated by high-speed centrifugation, leaving a mixture of plasma and reagent (the substance to be detected) in the light-transmitting detection zone. And then the detection device 30 collects and processes the luminous signal intensity of the substance to be detected, so as to obtain a detection result. After the detection device 30 detects the substance to be detected in one of the reagent cards 60, the positioning device 20 cooperates with the driving device 70 to change the position of the reagent card 60, and then the detection device 30 detects the substance to be detected on the other reagent card 60.

In the present application, the detection device 30 is an existing detection device, and the type of the detection device can be adjusted by different reagents and different detection samples, which is not limited in this application. For example: the reagent may be an acceptor reagent and a donor reagent, wherein the donor reagent comprises a donor capable of generating singlet oxygen in an excited state; the acceptor reagent includes an acceptor that is capable of reacting with singlet oxygen to produce a detectable chemiluminescent signal value.

Alternatively, the acceptor is a polymeric microparticle filled with a luminescent compound and a lanthanide compound. For example: the luminescent compound is selected from olefin compounds, which may be selected from the group consisting of dimethylthiophene, bisbutanedione compounds, dioxines, enol ethers, enamines, 9-alkylene xanthenes, 9-alkylene-N-9, 10-dihydroacridine, aryletherenes, arylimidazoles and lucigenin, and their derivatives, such as dimethylthiophene and its derivatives. For example: the lanthanide compound is a europium complex. The receptor binds directly or indirectly to the target molecule to be detected (target molecule contained in the test sample) to form a label.

Alternatively, the donor is a polymer particle filled with a photoactive compound that can generate singlet oxygen upon excitation by a red laser. For example: the photosensitive compound is selected from one of methylene blue, rose bengal and phthalocyanine. The donor is directly or indirectly bound to the label. The target molecule to be detected is an antigen or an antibody; wherein an antigen refers to a substance having immunogenicity, such as: protein, polypeptide. Representative antigens include: cytokines, tumor markers, metalloproteins, cardiovascular-diabetes-related proteins, and the like. Antibodies refer to immunoglobulins produced by the body that recognize a particular foreign body. Antibodies refer to immunoglobulins produced by the body that recognize a particular foreign body. For example: alpha Fetoprotein (AFP), HBsAb hepatitis B virus surface antibody (HBsAb), human chorionic gonadotrophin and beta subunit (HCG+beta), hepatitis B surface antigen (HBsAg), cancer antigen 125 (CA 125), C Peptide (CP), ferritin (Ferr), anti-HCV, etc.

With continued reference to fig. 1 and 2, the homogeneous chemiluminescent detection system further comprises a base plate 40 and a housing 50, the base plate 40 and the housing 50 forming a detection chamber, that is, the base plate 40 and the housing 50 cooperate to form a cavity in which the holder 90, the centrifuge 10, the positioning device 20, the drive device 70 and the detection device 30 are disposed. Of course, all structures of the centrifugal device 10, the positioning device 20, the driving device 70 and the detecting device 30 may be located in the cavity, or part of the structures may be located in the cavity, and part of the structures may be located outside the cavity, so long as the detection of the substance to be detected can be achieved.

With continued reference to fig. 4, the centrifuge apparatus 10 is rotatably mounted to the support 90, and the centrifuge apparatus 10 has a plurality of stations 113 for mounting the reagent cards 60, one reagent card 60 being mounted to each station 113. The centrifugal device 10 is preset with a working position 112, the detection device 30 is arranged corresponding to the working position 112 of the centrifugal device 10, and when the reagent card 60 is arranged at the working position 112, the detection device 30 can be used for collecting and processing the luminous signal intensity of the substance to be detected in the reagent card 60 at the working position 112.

That is, the detecting device 30 corresponds to the preset working position 112 of the centrifugal device 10, so as to detect the substance to be detected in the reagent card 60 at the working position 112. If the centrifuge 10 is rotated at a low speed (e.g., 5 r/min), the other reagent card 60 at station 113 is rotated at a low speed to the working position 112 so that the substance to be detected in the other reagent card 60 can be detected by the detecting means 30.

Fig. 5 is an exploded view of the centrifugal device 10 in the homogeneous chemiluminescent detection system according to the embodiment of the present application, and fig. 6 is a schematic structural view of the positioning device 20 in the homogeneous chemiluminescent detection system according to the embodiment of the present application; FIG. 7 is a cross-sectional view of a positioning device 20 in a homogeneous chemiluminescent detection system according to an embodiment of the present application. Referring to fig. 4 to 7, the centrifugal device 10 is further provided with clamping points 123 corresponding to the stations 113 one by one. The drive means 70 is drivingly connected to the centrifuge apparatus 10 to rotate a station 113 of the centrifuge apparatus 10 to the operating position 112. The positioning device 20 comprises a driving member 211, an elastic member 212 and a positioning pin 220, wherein the elastic member 212 is arranged on the positioning pin 220, and the driving member 211 is matched with the elastic member 212, so that the positioning pin 220 has a first state of being separated from the clamping point 123, a second state of being abutted against the centrifugal device 10 and a third state of being clamped in the clamping point 123.

After the detection device 30 detects the substance to be detected in the reagent card 60 at the working position 112, the positioning pin 220 is separated from the clamping point 123, the driving device 70 drives the centrifugal device 10 to rotate, at this time, the positioning pin 220 abuts against the centrifugal device 10, when one of the stations 113 rotates to the working position 112, the positioning pin 220 is clamped at the clamping point 123, the driving device 70 can stop driving the centrifugal device 10 to rotate through clamping of the positioning pin 220, at this time, a new reagent card 60 is placed at the working position 112, and then the detection device 30 detects the substance to be detected in the new reagent card 60 at the working position 112 again. The system does not need to use a servo motor to accurately position the rotation condition of the centrifugal device 10, can use a common motor to accurately position the rotation condition of the centrifugal device 10 by matching with the positioning device 20, and can reduce the cost of the homogeneous phase chemiluminescence detection system.

Before the station 113 rotates, the driving member 211 is not operated, and the elastic member 212 makes the positioning pin 220 in the first state; when the work stations 113 are not rotated to the working positions 112, the driving piece 211 drives the positioning pins 220 to be in the second state, and when one work station 113 is rotated to the working positions 112, the driving piece 211 drives the positioning pins 220 to be in the third state.

After the detection device 30 detects the substance to be detected in the reagent card 60 at the working position 112, the driving member 211 does not work, the elastic member 212 makes the positioning pin 220 separate from the clamping point 123 (the positioning pin 220 is in the first state), the driving device 70 drives the centrifugal device 10 to rotate, at this time, the station 113 does not rotate to the working position 112, due to the action of the driving member 211, the driving member 211 drives the positioning pin 220 to abut against the centrifugal device 10 (the positioning pin 220 is in the second state), when one of the stations 113 rotates to the working position 112, the driving member 211 drives the positioning pin 220 to clamp at the clamping point 123 (the positioning pin 220 is in the third state), and through the clamping of the positioning pin 220, the driving device 70 can stop driving the centrifugal device 10 to rotate, and at this time, a new reagent card 60 is placed at the working position 112.

Referring to fig. 4 and 5, the centrifugal apparatus 10 includes a centrifugal disk 111, a heating film 122, and a circuit board 121, which are disposed in this order from top to bottom. The centrifugal disc 111, the heating film 122 and the circuit board 121 are of sheet-shaped structures, and the centrifugal disc 111, the heating film 122 and the circuit board 121 synchronously rotate at high speed during centrifugation, so that the centrifugal effect of substances in the reagent card 60 is good, substances to be detected can be obtained, and the eccentricity is not easy to occur in the high-speed centrifugation process, so that the service life of equipment can be prolonged to a certain extent. After the centrifugation is completed, the heating film 122 is energized through the wiring board 121 so that the heating film 122 incubates and holds the substance to be detected in the reagent card 60 for a while. The detection device 30 is then used to detect the substance to be detected in the incubation.

With continued reference to fig. 3 to 5, the driving device 70 is a centrifugal motor, and the centrifugal motor is disposed below the centrifugal device 10, and the centrifugal motor 70 drives the circuit board 121 and the centrifugal disk 111 to rotate at a high speed, so that the circuit board 121 and the centrifugal disk 111 can rotate at a high speed, and the heating film 122 correspondingly interposed between the circuit board 121 and the centrifugal disk 111 also rotates at a high speed, so that the whole centrifugal device 10 can rotate at a high speed, and substances in the reagent cards 60 carried on the centrifugal disk 111 can be centrifuged at a high speed to obtain substances to be detected. Alternatively, the centrifugal motor may be a conventional motor and a servo motor may not be used, thereby saving the cost of the homogeneous chemiluminescent detection system.

With continued reference to fig. 4 and 5, the centrifugal disk 111 is not limited to a disk-shaped structure, and may be provided with a notch 1231, a through hole, or the like, or a clamping member for fixing the reagent card 60, or the like, alternatively, the centrifugal disk 111 is of a central symmetry structure, so that the centrifugal effect is better during high-speed centrifugation.

With continued reference to fig. 4 and 5, the centrifuge disk 111 has a plurality of stations 113 for mounting reagent cards 60; a station 113 may be provided with a reagent card 60, and a test sample may be placed in the reagent card 60 to test it. Typically, the number of stations 113 is a double number so that eccentricity is less likely to occur during high speed centrifugation. For example: in the present application, the centrifugal discs 111 have six stations 113, six reagent cards 60 can be mounted on each centrifugal disc 111, and one reagent card 60 is mounted on one station 113; in other embodiments, twelve stations 113, four stations 113, sixteen stations 113, etc. may also be provided on the centrifuge disk 111, and the present application is not limited thereto. In the present application, the mounting manner of the reagent card 60 is not limited, and any arrangement capable of fixing the reagent card 60 to the station 113 is within the scope of the present application.

With continued reference to fig. 3 and 5, the circuit board 121 is provided with a plurality of clamping points 123, and when the positioning device 20 is clamped at one of the clamping points 123, one of the reagent cards 60 on the centrifugal disk 111 is located at the working position 112. When the positioning device 20 is snapped onto the other snap point 123, the other reagent card 60 on the centrifuge disk 111 is located at the working position 112. When the circuit board 121 is rotated and the reagent card 60 is not yet located at the working position 112, the positioning pins 220 abut against the circuit board 121.

With continued reference to fig. 5 to 7, the edge of the circuit board 121 is provided with notches 1231 corresponding to the stations 113 one by one, and the positioning pins 220 are selectively clamped in one notch 1231; or the lower surface of the circuit board 121 is provided with clamping holes corresponding to the stations 113 one by one, and the positioning pins 220 are alternatively clamped in one clamping hole.

When a reagent card 60 is located at the working position 112, the positioning pin 220 is clamped in a notch 1231 or a clamping hole, after the detecting device 30 detects a substance to be detected in the reagent card 60 at the working position 112, the elastic member 212 drives the positioning pin 220 to separate from the clamping point 123, and then the centrifugal motor drives the circuit board 121 and the centrifugal disc 111 to rotate at a low speed, and at this moment, in the rotating process, the driving member 211 drives the upper end of the positioning pin 220 to abut against the lower surface of the circuit board 121; when the other notch 1231 or the clamping hole rotates to the position of the positioning pin 220 at a low speed, the driving piece 211 continues to drive the positioning pin 220 to ascend and clamp at the other notch 1231 or the clamping hole, the centrifugal motor does not rotate any more, at this time, the new reagent card 60 rotates to the working position 112 at a low speed, and then the detection device 30 continues to detect the substance to be detected of the new reagent card 60 at the working position 112.

With continued reference to fig. 6 and 7, the positioning device 20 further includes a sleeve 216, the elastic member 212 is a coil spring 213, the coil spring 213 is sleeved outside the positioning pin 220, an upper section of the coil spring 213 is disposed in the sleeve 216, and a lower end of the coil spring 213 is fixed to the positioning pin 220 and an upper end is fixed to the driving member 211. The sleeve 216 can facilitate the installation of the positioning pin 220 and the coil spring 213, and simultaneously, when the driving member 211 is not in operation, the lower end of the positioning pin 220 is conveniently moved downwards by the rebound action of the coil spring 213, so that the positioning pin 220 is separated from the locking point 123. Optionally, the positioning device 20 further includes a fixing base 217, where the fixing base 217 is fixed on the support 90, and the sleeve 216 is fixed in the positioning base, so that the fixing of the sleeve 216 can be facilitated. In another embodiment, the elastic member 212 may also be a spring plate.

With continued reference to fig. 6 and 7, the driving member 211 is an electromagnet 214, the electromagnet 214 is disposed at the lower end of the positioning pin 220, and a magnetic core 2141 of the electromagnet 214 contacts with the lower end of the positioning pin 220, when the electromagnet 214 is energized, the magnetic core 2141 of the electromagnet 214 moves upward and moves upward synchronously with the positioning pin 220; when electromagnet 214 is de-energized, coil spring 213 springs back to disengage detent 220 from catch 123.

When electromagnet 214 is energized, magnetic core 2141 of electromagnet 214 moves upward, positioning pin 220 in contact with the magnet of electromagnet 214 also moves upward, coil spring 213 is in a compressed state, and the upper end of positioning pin 220 can abut against the lower surface of circuit board 121; when the locking point 123 rotates to the position of the positioning pin 220, the magnetic core 2141 continues to drive the positioning pin 220 to move upwards, so that the upper end of the positioning pin 220 is locked to the locking point 123; when electromagnet 214 is de-energized, core 2141 of electromagnet 214 no longer exerts a force on dowel 220, and coil spring 213 springs back, causing dowel 220 to move downward away from stuck point 123 (the state shown in FIG. 7 is the state in which electromagnet 214 is de-energized). With continued reference to fig. 6 and 7, the driving device 70 further includes a zero position photo 218, where the zero position photo 218 is disposed on the driving member 211 for detecting whether the positioning pin 220 is separated from the locking point 123. The zero position photoelectric 218 can determine the position of the positioning pin 220 to determine whether the driving device 70 can drive the centrifugal device 10 to rotate, so as to avoid unnecessary limitation of the rotation of the centrifugal device 10 by the positioning pin 220.

In one embodiment, driving member 211 is an electromagnet 214, and a zero position photoelectric device 218 is disposed at a lower end of electromagnet 214 for detecting whether electromagnet 214 is energized. If the zero photoelectric 218 detects that the electromagnet 214 is powered off, the positioning pin 220 is in a state of being separated from the clamping point 123, and the centrifugal disc 111 and the circuit board 121 can be controlled to rotate at a low speed through a centrifugal motor, or the centrifugal disc 111 can be controlled to rotate at a high speed through the centrifugal motor; if electromagnet 214 is energized, locating pin 220 is in a state of being snapped into snap-in point 123, and then it is necessary to first de-energize electromagnet 214 and then control the rotation of the centrifugal motor.

Optionally, null photo 218 is a current sensor, and null photo 218 is disposed between electromagnet 214 and the circuit board, and if the current sensor detects a current, it indicates that a current is flowing through electromagnet 214, and electromagnet 214 is energized; if the current sensor does not detect a current, it indicates that no current is flowing through electromagnet 214 and electromagnet 214 is de-energized.

In other embodiments, the driving member may drive the positioning pin to be separated from the clamping point (the positioning pin is in the first state) before the station rotates; when the station is not rotated to the working position, the driving piece does not work, the elastic piece drives the positioning pin to abut against the lower surface of the circuit board (the positioning pin is in the second state), and when one station is rotated to the working position, the elastic piece continues to drive the positioning pin to be clamped at the clamping point (the positioning pin is in the third state).

The driving piece is an electromagnet, the electromagnet is arranged at the lower end of the positioning pin, and when the electromagnet is electrified, the electromagnet attracts the positioning pin to enable the positioning pin to move downwards to be separated from the clamping point (the positioning pin is made of magnetic materials); when the electromagnet is powered off, the spiral spring rebounds to enable the upper end of the positioning pin to be clamped at the clamping point or enable the upper end of the positioning pin to be abutted against the lower surface of the circuit board.

In general, the positioning pin is made of magnetic materials, and when the electromagnet is electrified, the electromagnet can attract substances made of the magnetic materials, so that the coil spring and the positioning pin fixed on the coil spring can move downwards, and the positioning pin is separated from the clamping point. When the electromagnet is powered off, the magnetism of the electromagnet disappears, and the positioning pin can rebound due to the action of the spiral spring, so that the positioning pin is clamped on a new clamping point or the upper end of the positioning pin is abutted against the lower surface of the circuit board. Through the setting of electro-magnet and coil spring, conveniently control the locating pin and rise or descend to adjust the card of locating pin and establish the position, simultaneously, the cost of electro-magnet is lower.

Optionally, a blocking piece is arranged outside the magnetic core of the electromagnet, the upper end of the spiral spring is fixed on the locating pin, and the lower end of the spiral spring abuts against and is fixed on the blocking piece. When the electromagnet is electrified to attract the positioning pin, the positioning pin is contacted with the electromagnet magnetic core, and the spiral spring is in a compressed state; when the electromagnet is powered off, the force of the magnetic core of the electromagnet to the attraction of the locating pin disappears, the spiral spring rebounds to enable the locating pin to be propped against the lower surface of the circuit board, and when the clamping point of the circuit board rotates to the locating pin at a low speed, the spiral spring rebounds to enable the locating pin to move towards the direction away from the magnetic core, and the locating pin is clamped into the clamping point. Meanwhile, whether the locating pin is separated from the clamping point can still be detected through zero photoelectric detection.

In another embodiment, the driving member may be a linear driving member, where the linear driving member is disposed at the lower end of the positioning pin, and the linear driving member drives the coil spring and the positioning pin to move downward, so that the positioning pin is separated from the clamping point; or the linear driving piece drives the spiral spring and the locating pin to move, and the spiral spring rebounds, so that the upper end of the locating pin is abutted or clamped with the centrifugal device at the clamping point. For example: the motor screw nut matching device, the air cylinder, the hydraulic cylinder and the like are not limited in this application.

If the linear driving piece is an air cylinder, the piston rod of the air cylinder moves downwards, so that the spiral spring connected with the piston rod and the locating pin move downwards together, and the locating pin is separated from the clamping point. The centrifugal motor drives the centrifugal disc to rotate at a low speed, and meanwhile, the piston rod moves upwards to enable the spiral spring connected with the piston rod and the locating pin to move upwards together, the spiral spring compresses and the upper end of the locating pin abuts against the lower surface of the circuit board; the centrifugal motor continues to rotate at a low speed, and when the clamping point rotates to the positioning pin, the spiral spring rebounds to enable the positioning pin to be clamped at the clamping point, the centrifugal motor stops rotating under the action of the positioning pin, and the new reagent clamp is positioned at the working position 112.

The driving part is a linear driving part (such as a cylinder), and the zero position photoelectric device is arranged beside the piston rod and used for detecting the position of the piston rod. If the position of the piston rod is detected to be in a downward moving state by the zero photoelectric detection, the positioning pin is in a state of being separated from the clamping point, and the centrifugal disc and the circuit board can be controlled to rotate at a low speed through the centrifugal motor or the centrifugal disc can be controlled to rotate at a high speed through the centrifugal motor; if the position of the piston rod is detected to be in an upward moving state by the zero photoelectric detection, the positioning pin is in a state of being clamped in the clamping point, and the piston rod needs to be controlled to move downwards before the centrifugal motor is controlled to rotate. Alternatively, the zero position photoelectric is a displacement sensor, which can detect the position of the piston rod.

The homogeneous chemiluminescent detection system provided by the application further comprises a control device 80, wherein the control device 80 is electrically connected with the centrifugal motor 70, the electromagnet 214, the zero photoelectric 218, the circuit board 121 and the detection device 30.

The working principle of the homogeneous chemiluminescent detection system provided by the embodiment of the application is as follows: a test sample is loaded into each reagent card 60, and then a reagent card 60 is mounted on each station 113 of the centrifuge disk 111.

Zero position photoelectric 218 detects whether electromagnet 214 is energized, and if electromagnet 214 is energized, a signal is sent to control device 80, control device 80 controls electromagnet 214 to be de-energized, so that coil spring 213 resets control positioning pin 220 to move downward, and positioning pin 220 is disengaged from stuck point 123. If null photo 218 detects that electromagnet 214 is de-energized, no signal is sent to control device 80.

The centrifugal motor is controlled to rotate at a high speed (for example, the rotation speed is 3000 r/min-10000 r/min) by the control device 80, so that the centrifugal disk 111, the heating film 122, the circuit board 121 and the reagent card 60 on the centrifugal disk 111 all synchronously rotate, and the mixture of the receptor, the donor and the detection sample in the reagent card 60 is centrifuged to obtain a substance to be detected. Then, the controller 80 controls the energization of the wiring board 121, and energizes the heating film 122 electrically connected to the wiring board 121, thereby incubating the substance to be detected.

Control device 80 controls electromagnet 214 to be energized, magnetic core 2141 of electromagnet 214 is lifted, positioning pin 220 abutting against magnetic core 2141 is lifted to abut against the lower surface of circuit board 121, and control device 80 controls the centrifugal motor to rotate slowly, for example: when the clamping point 123 rotates to the position of the positioning pin 220, the electromagnet 214 continues to drive the positioning pin 220 to ascend, the positioning pin 220 is clamped at the clamping point 123, the centrifugal motor stops rotating, and the reagent card 60 is arranged at the working position 112. The control device 80 controls the detection device 30 to detect the substance to be detected of the reagent card 60 at the working position 112.

After the detection is finished, the control device 80 controls the electromagnet 214 to be powered off, the magnetic core 2141 of the electromagnet 214 does not have upward force on the positioning pin 220 any more, the spiral spring 213 is reset to enable the positioning pin 220 to be separated from the clamping point 123, then the centrifugal motor controls the centrifugal disc 111 and the circuit board 121 to rotate slowly, the control device 80 controls the electromagnet 214 to be powered on, the magnetic core 2141 of the electromagnet 214 rises to enable the positioning pin 220 to abut against the lower surface of the circuit board 121, and when the other clamping point 123 rotates to the positioning pin 220, the magnetic core 2141 of the electromagnet 214 continues to rise to enable the positioning pin 220 to be clamped in the clamping point 123, and the new reagent card 60 is switched to the working position 112. The control means 80 control the detection means 30 to detect the substance to be detected in the incubation of the reagent card 60 at this working position 112. And so on to detect the substances to be detected in the different reagent cards 60 at the working position 112 one by one.

The homogeneous chemiluminescent detection system provided by the embodiment of the application has the following beneficial effects:

(1) Through the cooperation of the positioning pin 220, the driving piece 211 and the elastic piece 212, the accurate positioning between the reagent card 60 and the detection device 30 on different stations 113 can be realized by using a common motor, and the cost of the homogeneous chemiluminescence detection system is reduced.

(2) Through the cooperation of the coil spring 213 and the driving piece 211, the coil spring 213 resets to enable the positioning pin 220 to be separated from the clamping point 123, the driving piece 211 drives the positioning pin 220 to be clamped in the clamping point 123 or the upper end of the positioning pin 220 abuts against the lower surface of the circuit board 121, so that the positioning pin 220 is separated from or clamped with the clamping point 123, and the clamping position of the positioning pin 220 is conveniently switched.

(3) By energizing the electromagnet 214, the positioning pin 220 is controlled to clamp the clamping point 123 or the upper end of the positioning pin 220 is abutted against the lower surface of the circuit board 121, so that the positioning pin 220 can be controlled more conveniently, and the electromagnet 214 is lower in cost.

The foregoing description is only of the preferred embodiments of the present application and is not intended to limit the same, but rather, various modifications and variations may be made by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims (10)

1. A homogeneous chemiluminescent detection system comprising:

the bracket is arranged on the upper surface of the bracket,

the centrifugal device is rotatably arranged on the bracket, a working position for detection is preset on the centrifugal device, and a plurality of stations for installing reagent cards and clamping points corresponding to the stations one by one are arranged on the centrifugal device;

the driving device is in driving connection with the centrifugal device so as to enable one station of the centrifugal device to rotate to the working position;

the positioning device comprises a driving piece, an elastic piece and a positioning pin, wherein the elastic piece is arranged on the positioning pin, the driving piece is matched with the elastic piece, so that the positioning pin has a first state of being separated from a clamping point, a second state of being abutted against the centrifugal device and a third state of being clamped in the clamping point.

2. The homogeneous chemiluminescent detection system of claim 1 wherein the drive member is deactivated prior to rotation of the station, the resilient member placing the dowel in the first state; when the work stations are not rotated to the working positions, the driving piece drives the positioning pins to be in the second state, and when one work station is rotated to the working positions, the driving piece drives the positioning pins to be in the third state.

3. The homogeneous chemiluminescent detection system of claim 2 wherein the locating device further comprises a sleeve, the resilient member is a coil spring, the coil spring is disposed around the locating pin, an upper section of the coil spring is disposed within the sleeve, a lower end of the coil spring is secured to the locating pin, and an upper end of the coil spring is secured to the sleeve.

4. A homogeneous chemiluminescent detection system of claim 3 wherein the drive member is an electromagnet disposed at the lower end of the locating pin and having a magnetic core in contact with the lower end of the locating pin, the magnetic core of the electromagnet moving upward and moving upward synchronously with the locating pin when the electromagnet is energized; when the electromagnet is powered off, the spiral spring rebounds to enable the locating pin to be separated from the clamping point.

5. The homogeneous chemiluminescent detection system of claim 1 wherein the drive member is a linear drive member disposed at a lower end of the locating pin, the linear drive member driving the resilient member and the locating pin downward such that the locating pin is disengaged from the clip point; or the linear driving piece drives the elastic piece and the locating pin to move, and the elastic piece rebounds, so that the upper end of the locating pin and the centrifugal device are abutted or clamped at the clamping point.

6. A homogeneous chemiluminescent detection system according to any one of claims 2 to 5 wherein the centrifugal device comprises a centrifugal tray, a heating film and a circuit board sequentially disposed from top to bottom, wherein the centrifugal tray has a plurality of stations thereon, the circuit board has a plurality of clamping points thereon, and the locating pin is selectively clamped at one of the clamping points or is abutted against the circuit board.

7. The homogeneous chemiluminescent detection system of claim 6 wherein the edge of the circuit board is provided with notches in one-to-one correspondence with the stations, the locating pins being selectively snapped into one of the notches;

or, the lower surface of the circuit board is provided with clamping holes corresponding to the stations one by one, and the positioning pins are alternatively clamped in one of the clamping holes.

8. A homogeneous chemiluminescent detection system according to any one of claims 1 to 5 wherein the drive device further comprises a zero position photoelectric device disposed on the drive for detecting whether the locating pin is disengaged from the clip point.

9. A homogeneous chemiluminescent detection system according to any one of claims 1 to 5 further comprising detection means for detecting and processing the luminescent signal intensity of a substance to be detected in the reagent card at the location of operation.

10. The homogeneous chemiluminescent detection system of claim 9 further comprising a base plate and a housing, the base plate and the housing forming a detection chamber, the rack, the centrifugation device, the detection device, the drive device, and the positioning device being disposed in the detection chamber.

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