CN219079507U - Portable aseptic inoculation box - Google Patents

Abstract

The utility model belongs to the technical field of experimental instrument equipment and specifically relates to a portable aseptic inoculation box is related to, including the inoculation box that has the inoculation chamber, the inoculation box has been seted up the silica gel pipe that supplies the bacterial bottle respectively and the silica gel pipe of fermentation cylinder wears to establish the inlet that gets into the inoculation chamber, the operation mouth in the inoculation chamber has been seted up to the lateral wall of inoculation box, and the inoculation box is provided with the alcohol burner of built-in inoculation chamber, and inlet channel has been seted up to the diapire of inoculation box, the inoculation box is provided with the air suction piece that forces the air current of inoculation intracavity from flowing down upwards. The application has the effect of improving the stability of the alcohol lamp burning the inoculation pipeline.

Description

Portable aseptic inoculation box

Technical Field

The application relates to the technical field of experimental instrument equipment, in particular to a portable sterile inoculation box.

Background

In the inoculation and culture process of a laboratory fermentation tank, a silicone tube on a strain bottle and a silicone tube of the fermentation tank are connected to form an inoculation pipeline for inoculation, seventy-five percent ethanol (alcohol) is required to be sprayed to disinfect an inoculation area before inoculation, and then an alcohol lamp is used for burning the inoculation pipeline to achieve a sterilization effect, and then inoculation operation is carried out.

With respect to the related art in the above, the inventors consider that there are the following drawbacks: however, in the process of burning the inoculation pipeline, the alcohol lamp is in an open environment and is easily influenced by wind power in the environment and breathing of an operator, so that the flame of the alcohol lamp swings, the stability of the flame of the alcohol lamp is reduced, and the alcohol lamp is easy to be extinguished in the sterilization operation process when the alcohol content is low; when the alcohol content is high, excessive fire is easily caused, and the risk of burning to people exists, so that further improvement is needed.

Disclosure of Invention

In order to improve the stability of burning an alcohol lamp to a seed pipeline, the application provides a portable sterile seed box.

The application provides a portable aseptic inoculation box adopts following technical scheme:

the utility model provides a portable aseptic inoculation box, includes the inoculation box that has the inoculation chamber, the inoculation box has been seted up the silica gel pipe that supplies the bacterial bottle respectively and the silica gel pipe of fermentation cylinder wears to establish the inlet that gets into the inoculation chamber, the operation mouth in the inoculation chamber is seted up to the lateral wall of inoculation box, and the inoculation box is provided with the alcohol burner of putting in the inoculation chamber, and inlet channel has been seted up to the diapire of inoculation box, the inoculation box is provided with the air suction piece that forces the air current in the inoculation chamber from down upwards flowing.

Through adopting above-mentioned technical scheme, through setting up the inoculation box, provide relatively confined inoculation environment, when carrying out inoculation operation, wear the silica gel tube of bacterial bottle and the silica gel tube of fermentation cylinder earlier and locate into the thing mouth, operator's hand stretches into inoculation intracavity through the operation mouth, burn sterilization to the silica gel tube of bacterial bottle and the silica gel tube of fermentation cylinder through handheld alcohol burner, start the piece of bleeding and draw the gas that makes inoculation intracavity from the air current that has down from last, on the one hand, provide oxygen for the burning of alcohol burner, on the other hand, reduce the influence of external environment to the alcohol burner, thereby reduce the possibility that the flame of alcohol burner takes place the side-to-side swing, improve the stability of burning of alcohol burner butt joint seed pipeline.

Preferably, the inside wall of the material inlet is provided with a connecting sheet which is used for sealing the material inlet in a normal state, the connecting sheet is provided with a plurality of connecting sheets which are distributed around the axis of the material inlet, and the connecting sheet is elastically arranged.

Through adopting above-mentioned technical scheme, when inoculation box is in non-operating condition, a plurality of connection piece seals the thing mouth that advances, reduce impurity entering inoculation box inner chamber's possibility, when carrying out inoculation operation, with the silica gel pipe end butt in the free end of connection piece of the silica gel pipe of bacterial bottle/fermentation cylinder, and apply force to the silica gel pipe, the silica gel pipe end forces the connection piece to take place elastic bending, make the thing mouth be the intercommunication state, accomplish the wearing of silica gel pipe and establish the back, the connection piece has the elastic damping power to the silica gel pipe, reduce the possibility that the silica gel pipe takes place random slip.

Preferably, the inoculation box body is provided with a sterilizing device for sterilizing the inoculation cavity, and the sterilizing device comprises an ultraviolet lamp arranged on the inner wall of the top of the inoculation box body.

Through adopting above-mentioned technical scheme, be provided with ultraviolet lamp, the light that ultraviolet lamp transmitted can change microorganism gene structure, lets DNA impaired to let microorganism lose reproduction and restoration function, finally reach the inhibitory action of bacterium, sterilization effect is stable, makes inoculation chamber become stable aseptic district. In addition, the ultraviolet lamp is used for sterilization, so that the generation of odor is not worried, and any harmful chemical components are not remained.

Preferably, the top wall of the inoculation box body is provided with an air extraction opening, and the air extraction piece comprises an air extraction fan arranged in the air extraction opening.

By adopting the technical scheme, the air extraction fan works stably, so that the gas flow in the inoculation cavity is stable.

Preferably, the inoculation box is provided with a filter element covering the air inlet channel for filtering air.

Through adopting above-mentioned technical scheme, the filter element carries out separation filtration to the impurity in the air, reduces the possibility that impurity got into inoculation chamber, improves the cleanliness factor in inoculation chamber.

Preferably, the side wall of the inoculation box body is provided with an access hole, and the inoculation box body is provided with an access cover plate for controlling the opening and closing of the access hole.

Through adopting above-mentioned technical scheme, before carrying out inoculation operation, open the access hole, the operator sprays alcohol to the inside of inoculation box through the access hole and disinfects, improves the bactericidal effect, and during inoculation operation, the access hole shutoff is covered to the rethread.

Preferably, the access cover plate is hinged to the side wall of the inoculation box body, the free end of the access cover plate is detachably connected to the inoculation box body through a hasp, the protrusion of the inner side wall of the access opening is provided with a limiting edge, and the protrusion of the inner plate surface of the access cover plate is provided with a sealing gasket which is abutted to the limiting edge.

Through adopting above-mentioned technical scheme, realize opening and close of access hole fast through the hasp, the access panel is provided with sealing washer, improves the connection leakproofness between access panel and the spacing border.

Preferably, the inoculation box is a transparent box.

By adopting the technical scheme, the inoculation operation is convenient for operators.

Preferably, two operation ports are arranged, and operation gloves are arranged in the operation ports.

Through adopting above-mentioned technical scheme, be provided with the operation gloves, on the one hand, reduce the operator hand and inoculation pipeline direct contact's possibility, on the other hand, reduce the operator hand and take place the possibility of scalding, improve inoculation operation security.

Preferably, the protruding ring of the outer peripheral wall of the extending port of the operating glove is provided with a fixing ring, the diameter of the fixing ring is larger than that of the operating port, and the fixing ring is detachably connected to the side wall of the inoculation box body.

Through adopting above-mentioned technical scheme, the fixed ring can be dismantled and connect in the lateral wall of inoculation box, realizes then that the operation gloves can be dismantled with the inoculation box and be connected, is convenient for change the operation gloves of damage.

In summary, the utility model has the following beneficial effects:

1. the air extraction piece is arranged to extract air in the inoculation cavity, so that air flow from bottom to top is arranged in the inoculation cavity, on one hand, oxygen is provided for combustion of the alcohol lamp, and on the other hand, the alcohol lamp is in a relatively closed environment, so that the influence of the external environment on the alcohol lamp is reduced, the possibility of left-right swing of flame of the alcohol lamp is reduced, and the burning stability of the alcohol lamp to an inoculation pipeline is improved;

2. the sterilization effect is stable, is not influenced by ambient factors such as temperature, personnel and the like, and is suitable for being used in various environments such as laboratories, laminar flow rooms, clean benches and the like;

3. the fixed ring is detachably connected to the side wall of the inoculation box body, and then the detachable connection of the operation glove and the inoculation box body is realized, so that the damaged operation glove can be replaced conveniently.

Drawings

FIG. 1 is a schematic view showing the structure of a portable sterile inoculation box according to embodiment 1;

FIG. 2 is a schematic view showing the back structure of the inoculation box in example 1;

FIG. 3 is a schematic view of the construction of the access panel and the operating glove of example 1;

FIG. 4 is a schematic view showing the connection structure of the operation glove and the front plate in example 2;

fig. 5 is an enlarged partial schematic view of fig. 4 at a.

In the figure, 1, inoculating a box body; 11. a bottom plate; 111. an air intake passage; 12. a side plate; 13. a back plate; 14. a top plate; 141. a first handle; 142. a material inlet; 143. a connecting sheet; 144. an extraction opening; 15. a front plate; 151. an operation port; 16. a transition sloping plate; 161. an access opening; 162. a limit edge; 2. an alcohol lamp; 3. an air extraction fan; 4. an ultraviolet lamp; 5. a gas permeable filtration membrane; 6. an access panel; 61. a second handle; 62. a sealing gasket; 7. an operating glove; 71. a fixing ring; 72. a screw; 73. a mounting member; 731. a plug-in column; 732. a positioning head; 733. a deformation groove; 734. a guide slope; 8. a mounting groove; 81. inserting through holes; 82. and a limiting blind hole.

Detailed Description

The present application is described in further detail below in conjunction with figures 1-5.

Example 1:

the embodiment of the application discloses portable aseptic inoculation box, refer to fig. 1, fig. 2, including inoculation box 1 that has inoculation chamber, inoculation box 1 includes bottom plate 11, a pair of respectively fixed connection is in the curb plate 12 of bottom plate 11 both sides up end, fixed connection is in the backplate 13 of bottom plate 11 and fixed connection in curb plate 12, be located the top of bottom plate 11 and fixed connection in backplate 13 and curb plate 12 up end roof 14, fixed connection is kept away from backplate 13 one side up end and fixed connection in the front bezel 15 of curb plate 12 and be the slope setting and fixed connection in the transition swash plate 16 between front bezel 15 and the roof 14 in bottom plate 11, transition swash plate 16 fixed connection in curb plate 12. The bottom plate 11, the side plates 12, the back plate 13, the top plate 14, the front plate 15 and the transition sloping plate 16 are all acrylic plates, so that the inoculation box 1 is a transparent box.

Referring to fig. 1 and 3, the inoculation box 1 is provided with an alcohol lamp 2 built in an inoculation chamber and an ignition member (not shown) for igniting the alcohol lamp 2, which may be a lighter. The first handle 141 of up end fixedly connected with of roof 14 to be convenient for carry inoculation box 1, the up end of roof 14 has seted up two silicone tubes that supply the bacterial bottle respectively and the silicone tube of fermentation cylinder and has worn to establish the business turn over thing mouth 142 that gets into the inoculation chamber, and thing mouth 142 is from the resilience seal mouth, specifically, the inside wall of business turn over thing mouth 142 is provided with carries out confined connection piece 143 to business turn over mouth 142 under the normality, connection piece 143 is provided with a plurality of and winds the axis distribution of business turn over mouth 142, connection piece 143 is elasticity setting. When inoculation operation is carried out, the end part of the silica gel tube of the strain bottle or the silica gel tube of the fermentation tank is abutted against the free end of the connecting sheet 143, and force is applied to the silica gel tube, the end part of the silica gel tube forces the connecting sheet 143 to elastically bend, so that the material inlet 142 is in a communicating state, and the silica gel tube penetrates into the material inlet 142.

The bottom plate 11 is provided with an air inlet channel 111, the air inlet end of the air inlet channel 111 extends to the outer side wall of the bottom plate 11, the air outlet end of the air inlet channel 111 extends to the upper end face of the bottom plate 11, and the air inlet channel 111 is provided with a plurality of air inlets and uniformly distributed on the bottom plate 11. The upper end surface of the middle part of the top plate 14 is provided with an air extraction opening 144 in a penetrating way, the top plate 14 is provided with an air extraction piece forcing the air flow in the inoculation cavity to flow from bottom to top, and in the embodiment, the air extraction piece is an air extraction fan 3 built in the air extraction opening 144. The inoculation box 1 is provided with a filter element covering the air inlet channel 111 for filtering air, in this embodiment, the filter element is a ventilation filtering membrane 5 fixedly laid on the outer side wall of the bottom plate 11 for covering the air inlet port of the air inlet channel 111.

The top plate 14 is provided with a sterilizing device for sterilizing the inoculation chamber, in this embodiment, the sterilizing device is an ultraviolet lamp 4 fixedly connected to the lower end surface of the top plate 14, the ultraviolet lamps 4 are provided with two ultraviolet lamps and distributed on two sides of the air extraction opening 144, and the ultraviolet lamps 4 are located between the air extraction opening 144 and the material inlet 142.

The front plate 15 is penetrated and provided with two operation ports 151 communicated with the inoculation cavity, and the operation ports 151 are internally provided with operation gloves 7. The operating glove 7 is detachably connected to the front panel 15. The protruding ring of the outer peripheral wall of the extending opening of the operating glove 7 is provided with a fixing ring 71, the diameter of the fixing ring 71 is larger than that of the operating opening 151, the fixing ring 71 is detachably connected to the side wall of the inoculation box 1, and in this embodiment, the fixing ring 71 is threaded with a screw 72 screwed to the front plate 15.

The access hole 161 has been seted up to the lateral wall of inoculation box 1, inoculation box 1 is provided with the access panel 6 that control access hole 161 was opened and close, and access panel 6 is the ya keli board, and the inside wall of access hole 161 encircles and is fixed with spacing border 162 for access hole 161 is the heavy groove setting, and the upper portion of access panel 6 articulates in the inside wall of access hole 161, and the free end of access panel 6 can be dismantled through the hasp and connect in inoculation box 1. The outer plate surface of the access cover plate 6 is fixedly connected with a second handle 61, and the inner plate surface of the access cover plate 6 is provided with a protruding ring which is provided with a sealing gasket 62 which is abutted against a limiting edge 162, so that the tightness of the access cover plate 6 to the access hole 161 is improved.

The implementation principle of the portable sterile inoculation box provided by the embodiment of the application is as follows: before use, the access cover plate 6 is opened, after the inoculation cavity is sprayed and disinfected by seventy five percent ethanol through the access hole 161, the access cover plate 6 is closed, the ultraviolet lamp 4 is opened for irradiation sterilization, the air extraction fan 3 is started, the air in the inoculation cavity is extracted to enable the air in the inoculation cavity to be provided with air flow from bottom to top, the filtering membrane filters the air entering the inoculation cavity, after 15 minutes, the strain bottle silicone tube and the fermentation tank silicone tube penetrate into the inlet 142, hands of a worker extend into the operation hole 151, the operation is performed through the operation glove 7, the alcohol lamp 2 is ignited through the ignition piece, the strain bottle silicone tube and the fermentation tank silicone tube are connected after being burnt and sterilized, and finally the inoculation operation is completed. The alcohol lamp 2 is in a relatively closed environment, so that the influence of the external environment on the alcohol lamp 2 is reduced, the possibility that the flame of the alcohol lamp 2 swings left and right is reduced, and the burning stability of the alcohol lamp 2 to the inoculation pipeline is improved.

Example 2:

referring to fig. 4 and 5, the difference from embodiment 1 is that the outer plate surface of the front plate 15 is provided with a plurality of mounting grooves 8 surrounding the operation opening 151, the mounting grooves 8 include insertion through holes 81 and limit blind holes 82, the insertion through holes 81 are provided on the outer plate surface of the front plate 15, the limit blind holes 82 are communicated with the insertion through holes 81, and the diameter of the limit blind holes 82 is larger than that of the insertion through holes 81. The fixed ring 71 is correspondingly provided with the mounting piece 73 which is clamped and embedded in the mounting groove 8, the mounting piece 73 comprises a plug post 731 and a limiting head 732, one end of the plug post 731 is fixedly connected with the end face of the fixed ring 71, which is close to the front plate 15, the plug post 731 is adapted to be inserted into the limiting through hole, the limiting head 732 is coaxially and fixedly connected with the other end of the plug post 731, the limiting head 732 is clamped and embedded in the limiting blind hole 82, and in the embodiment, the fixed ring 71, the plug post 731 and the limiting head 732 are integrally formed and arranged and are rubber products. The end surface of the limiting head 732 far away from the plug connector is provided with a deformation groove 733 extending to the plug post 731, and the outer peripheral wall of one end of the limiting head 732 far away from the plug post 731 is provided with a guide inclined surface 734.

The implementation principle of the portable sterile inoculation box provided by the embodiment of the application is as follows: when the operation glove 7 is installed, the fixing ring 71 is applied with an acting force close to the front plate 15, the limiting head 732 is forced to elastically deform, when the limiting head 732 is completely inserted into the limiting blind hole 82, the limiting head 732 deforms and resets, the end face of the limiting head 732 close to the plug-in post 731 is abutted against the step end face between the limiting blind hole 82 and the plug-in through hole 81, and when the fixing ring 71 needs to be disassembled, the fixing ring 71 is applied with an acting force in the direction away from the front plate 15, so that the limiting head 732 is separated from the limiting blind hole 82.

The foregoing are all preferred embodiments of the present application, and are not intended to limit the scope of the present application in any way, therefore: all equivalent changes in structure, shape and principle of this application should be covered in the protection scope of this application.

Claims (10)

1. A portable sterile inoculation chamber, characterized in that: including inoculation box (1) with inoculation chamber, inoculation box (1) has been seted up the silica gel pipe that supplies the bacterial bottle respectively and has worn to establish into inoculation chamber's inlet (142) with the silica gel pipe of fermentation cylinder, inoculation box (1) lateral wall has been seted up and has been linked together in inoculation chamber's operation mouth (151), and inoculation box (1) is provided with built-in alcohol burner (2) in inoculation chamber, and inlet channel (111) have been seted up to inoculation box (1) diapire, inoculation box (1) is provided with the air suction piece that forces inoculation intracavity air current from down upwards flowing.

2. A portable sterile inoculation box according to claim 1, wherein: the inside wall of thing mouth (142) is provided with and carries out confined connection piece (143) to thing mouth (142) under the normality, and connection piece (143) are provided with a plurality of and around the axis distribution of thing mouth (142), and connection piece (143) are elasticity setting.

3. A portable sterile inoculation box according to claim 1, wherein: the inoculation box body (1) is provided with a sterilization device for sterilizing the inoculation cavity, and the sterilization device comprises an ultraviolet lamp (4) arranged on the inner wall of the top of the inoculation box body (1).

4. A portable sterile inoculation box according to claim 1, wherein: the top wall of the inoculation box body (1) is provided with an air extraction opening (144), and the air extraction piece comprises an air extraction fan (3) arranged in the air extraction opening (144).

5. A portable sterile inoculation box according to claim 1, wherein: the inoculation box body (1) is provided with a filter piece which covers the air inlet channel (111) to filter air.

6. A portable sterile inoculation box according to claim 1, wherein: the side wall of the inoculation box body (1) is provided with an access hole (161), and the inoculation box body (1) is provided with an access cover plate (6) for controlling the access hole (161) to be opened and closed.

7. A portable sterile inoculation box according to claim 6, wherein: the maintenance apron (6) articulates in inoculation box (1) lateral wall, and the free end of maintenance apron (6) can dismantle through the hasp and connect in inoculation box (1), and the inside wall protrusion of maintenance mouth (161) is provided with spacing border (162), and the inner panel face protrusion of maintenance apron (6) sets up the ring and is equipped with sealing washer (62) in butt in spacing border (162).

8. A portable sterile inoculation box according to claim 1, wherein: the inoculation box body (1) is a transparent box body.

9. A portable sterile inoculation box according to claim 1, wherein: two operation ports (151) are arranged, and operation gloves (7) are arranged in the operation ports (151).

10. A portable sterile inoculation box according to claim 9, wherein: the operation glove (7) stretches into mouthful perisporium bulge loop and is equipped with solid fixed ring (71), the diameter of solid fixed ring (71) is greater than the diameter of operation mouth (151), gu fixed ring (71) detachable connection is in the lateral wall of inoculation box (1).

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