CN219079487U

CN219079487U - Negative pressure suction filtration combines post installation device

Info

Publication number: CN219079487U
Application number: CN202223270477.0U
Authority: CN
Inventors: 庄胜宇
Original assignee: Guangzhou Feiyang Biological Engineering Co ltd
Current assignee: Guangzhou Feiyang Biological Engineering Co ltd
Priority date: 2022-12-05
Filing date: 2022-12-05
Publication date: 2023-05-26
Anticipated expiration: 2032-12-05

Abstract

The utility model relates to the technical field of nucleic acid extraction devices, in particular to a negative pressure suction filtration combined column mounting device, which comprises a frame plate, wherein a plurality of placing holes are formed in the frame plate, a protection pipe communicated with the placing holes is fixedly arranged below each placing hole at the bottom of the frame plate, and a through hole is formed in the bottom of the protection pipe; a detachable combination column is arranged in the protection tube, an exhaust port is formed in the bottom of the combination column, and the exhaust port passes through the through port to be communicated with the outside; an adsorption film is arranged above the discharge port in the binding column; a plurality of spliced poles can be placed on the frame plate and negative pressure suction filtration operation is carried out simultaneously, and improvement operating efficiency, the protective tube can protect its inside spliced pole simultaneously, avoids the spliced pole to expose in the external world when carrying out suction filtration operation, and then avoids the spliced pole to receive external impact.

Description

Negative pressure suction filtration combines post installation device

Technical Field

The utility model relates to the technical field of nucleic acid extraction devices, in particular to a negative pressure suction filtration combined column installation device.

Background

In the field of molecular biology research, it is often necessary to separate and purify large molecular substances such as DNA and RNA from biological samples. Therefore, rapid separation and purification of DNA and RNA nucleic acid materials is a key technology for improving the research efficiency of molecular biology. The use of silica gel purification columns to extract nucleic acid macromolecules has become a common means of molecular biology research. For the silica gel column nucleic acid extraction technology, there are two general methods: centrifugal separation and negative pressure suction separation. Centrifugation is currently the most commonly used in laboratories, and is performed as follows: cell samples are subjected to pyrolysis by using a lysate and then are centrifuged to obtain a supernatant; adding a certain proportion volume of nucleic acid column binding solution into the supernatant, mixing and transferring to a purification column; placing the purification column in a matched centrifuge tube, and centrifuging; taking out the purification column, and pouring out filtrate in the centrifuge tube; the purification column is put back into the original centrifuge tube again, nucleic acid washing liquid is added, and filtrate is removed by centrifugation; adding nucleic acid washing liquid into the purification column again, centrifuging and discarding filtrate (optional); the purification column is put back into the original centrifuge tube again, and the residual washing liquid on the purification column membrane is removed by centrifugation; and (3) placing the purification column back into a new centrifuge tube, adding a certain amount of nucleic acid eluent into the center of the membrane of the purification column, standing for a period of time, and centrifugally eluting to obtain the nucleic acid meeting the molecular biological quality requirement. In the centrifugation method, the purification column is required to be matched with the centrifuge tube for use for a plurality of times, and each time of centrifugation, the purification column is required to be subjected to the processes of taking out from the centrifuge tube and putting in again, so that the operation process is complex, and the treatment of a plurality of samples is not facilitated. The specific operation process of the negative pressure method is as follows: inserting the purification column onto an interface of a negative pressure device; cell samples are lysed by using lysate and the supernatant is taken; adding a certain proportion volume of nucleic acid column binding solution into the supernatant, mixing and transferring to a purification column; starting negative pressure equipment, and sucking liquid in the purification column; maintaining negative pressure, and adding washing liquid to wash the purification column; maintaining negative pressure, and removing residual washing liquid on the purification column membrane; placing the purification column into a new centrifuge tube, adding a certain amount of nucleic acid eluent into the center of the membrane of the purification column, standing for a period of time, and centrifugally eluting to obtain the nucleic acid meeting the molecular biological quality requirement. The negative pressure method has the advantages that the complicated centrifugal process in most centrifugal methods is replaced by using a negative pressure air suction mode, so that the whole operation process is time-saving and labor-saving, and a plurality of samples can be continuously separated and purified.

Some traditional negative pressure suction filtration equipment only can purify to a purification post (or called the combination post) at a time, and suction filtration operation is lower in efficiency.

The existing negative pressure suction filtration device comprises a main body which is provided with a purification column, a support pipe rack and a waste liquid collecting bottle respectively; wherein the upper part of the support pipe rack is a sample receiving port, and the lower part of the support pipe rack is a liquid discharge pipe; the sample receiving port is connected with the purification column through a first rubber plug and a needle head of a general-purpose syringe, and the liquid discharge pipe is connected with a waste liquid collecting bottle through a second rubber plug; the waste liquid collecting bottle is provided with an interface which is connected with the negative pressure device.

The prior negative pressure suction filtration device is easy to damage due to the fact that the combined column is exposed to the outside when in use.

Disclosure of Invention

The utility model provides a negative pressure suction filtration combined column installation device, which aims to solve the problems that the negative pressure suction filtration combined column in the prior art is low in efficiency and easy to damage in use.

In order to solve the technical problems, the utility model adopts the following technical scheme:

the pressure suction filtration combined column device comprises a frame plate, wherein a plurality of placing holes are formed in the frame plate, a protection tube communicated with the placing holes is fixedly arranged on the frame plate, and a through hole is formed in the bottom of the protection tube; a detachable combination column is arranged in the protection tube, the top of the combination column is opened, the bottom of the combination column is provided with a discharge port, and the discharge port passes through the through port to be communicated with the outside; and an adsorption film is arranged above the discharge port in the binding column.

The frame plate is used for erecting the combination column, and meanwhile, the whole device is also supported on the negative pressure suction filtration box by the frame plate. The combination post is placed into the protection pipe through placing the hole, and the protection pipe is fixed on the frame plate, so that the combination post in the protection pipe can be protected from being impacted by external force, and the combination post is prevented from being inclined and damaged due to the impact of the external force. Meanwhile, a plurality of binding columns can be placed on one frame plate, so that the operations of suction filtration and purification on the binding columns at one time are achieved; the through port is used for enabling the exhaust port to be communicated with the outside; the discharge port is used for discharging waste liquid in the binding column; the adsorption film is used for adsorbing biological samples required by operators, and simultaneously allows impurities to pass through the adsorption film along with washing liquid.

Further, the bottom of the combined column is provided with a column mouth, the exhaust port is arranged at the bottom of the column mouth, the column mouth penetrates through the passing port, and the outer diameter of the column mouth is equal to the inner diameter of the passing port.

Further, a sealing glue layer is arranged on the upper surface of the frame plate, and the combination column penetrates through the sealing glue layer and the placement hole and is movably inserted into the protection tube; the top of the binding post is provided with a flange, the outer diameter of the flange is larger than the inner diameter of the placing hole, and the bottom of the flange is abutted to the sealant layer.

Further, the locking mechanism capable of locking the binding post in the protection tube is arranged on the binding post and the protection tube.

Further, the locking mechanism comprises a plurality of sliding blocks and a plurality of limiting strips; the limiting strips are obliquely arranged on the inner wall of the protection tube, and the sliding blocks are arranged on the outer wall of the combination column; the limiting strip comprises a starting end and a finishing end, and the starting end is positioned above the finishing end; the sliding block is positioned below the starting end and above the finishing end, and the top of the sliding block is in sliding contact with the bottom of the limiting strip.

Further, the locking mechanism comprises a first thread and a second thread, the first thread is arranged on the inner wall of the protection tube, and the second thread is arranged on the outer wall of the combination column; the combination column is inserted into the protection tube through the cooperation of the first thread and the second thread.

Further, a plurality of deflector rods are arranged at the top of the combination column.

Further, adjacent protection pipes are connected through reinforcing ribs.

Further, an apron is arranged on the periphery of the frame plate, and extends towards the lower part of the frame plate; the bottom of the apron is provided with a sealing strip.

Further, the device also comprises a sealing plug which is detachably arranged in the placing hole.

Compared with the prior art, the utility model has the beneficial effects that:

1. through setting up a plurality of holes of placing on the frame plate, and the fixed protection tube that sets up and place the hole intercommunication in frame plate bottom for can place a plurality of spliced poles on a frame plate and carry out negative pressure suction filtration operation simultaneously, improve the operating efficiency, the spliced pole of inside can be protected to the protection tube simultaneously, avoids the spliced pole to expose in the external world when carrying out suction filtration operation, and then avoids the spliced pole to receive external impact;

2. the inner diameter of the through hole is equal to the outer diameter of the column mouth, so that when the combined column is inserted into the protection tube, the outer wall of the column mouth is just abutted against the inner wall of the through hole, the through hole can play a role in positioning and limiting the column mouth, the combined column is prevented from shaking in the protection tube, meanwhile, the gap between the combined column and the protection tube can be reduced, and the negative pressure suction filtration effect is prevented from being reduced due to the gap between the combined column and the protection tube when the negative pressure suction filtration is carried out;

3. the sealing glue layer is arranged on the frame plate, the flange is arranged at the top of the combining column, when the combining column is placed in the protective tube, the outer diameter of the flange is larger than the outer diameter of the placing hole, and the flange is abutted with the sealing glue layer positioned at the top of the frame plate, so that a sealing structure is formed, and when negative pressure suction filtration is carried out, external air is prevented from entering the frame plate and the suction filtration box through a gap between the combining column and the protective tube, so that a sealing structure cannot be formed between the frame plate and the suction filtration box, and the suction filtration effect is influenced;

4. through the arrangement of the locking mechanism, the binding post can be fixed in the protection tube through the locking mechanism, the stability of the binding post during suction filtration operation is ensured, and the binding post can be removed from the protection tube by operating the locking mechanism to unlock, so that the detachable effect is achieved; the setting can be dismantled to the spliced pole, and convenient change also can increase and decrease the quantity of spliced pole according to actual conditions.

Drawings

FIG. 1 is a view showing the state of use of example 1 of a negative pressure suction filtration binding column mounting device according to the present utility model;

FIG. 2 is a schematic view showing the overall structure of an embodiment 1 of a negative pressure suction filtration combined column mounting device according to the present utility model;

FIG. 3 is a schematic view showing the internal structure of an embodiment 1 of a negative pressure suction filtration combined column mounting device according to the present utility model;

FIG. 4 is a schematic view showing the internal structure of a protective tube in accordance with an embodiment 1 of the present utility model;

FIG. 5 is a schematic view showing the internal structure of an embodiment 2 of a negative pressure suction filtration combined column mounting device according to the present utility model;

fig. 6 is a use state diagram of embodiment 3 of a negative pressure suction filtration combined column mounting device of the present utility model.

In the accompanying drawings: 1. a frame plate; 2. placing the hole; 3. a protective tube; 4. a through port; 5. a binding column; 6. a discharge port; 7. an adsorption film; 8. a pillar mouth; 9. a sealant layer; 10. a flange; 11. a locking mechanism; 111. a slide block; 112. a limit bar; 1121. a starting end; 1122. a terminal end; 113. a first thread; 114. a second thread; 12. a deflector rod; 13. apron; 14. a sealing strip; 15. reinforcing ribs; 16. a sealing plug; 17. and a suction filtration box.

Detailed Description

The drawings are for illustrative purposes only and are not to be construed as limiting the present patent; for the purpose of better illustrating the embodiments, certain elements of the drawings may be omitted, enlarged or reduced and do not represent the actual product dimensions; it will be appreciated by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationship depicted in the drawings is for illustrative purposes only and is not to be construed as limiting the present patent.

The same or similar reference numbers in the drawings of embodiments of the utility model correspond to the same or similar components; in the description of the present utility model, it should be understood that, if there are orientations or positional relationships indicated by terms "upper", "lower", "left", "right", "long", "short", etc., based on the orientations or positional relationships shown in the drawings, this is merely for convenience in describing the present utility model and simplifying the description, and is not an indication or suggestion that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, so that the terms describing the positional relationships in the drawings are merely for exemplary illustration and are not to be construed as limitations of the present patent, and that it is possible for those of ordinary skill in the art to understand the specific meaning of the terms described above according to specific circumstances.

The technical scheme of the utility model is further specifically described by the following specific embodiments with reference to the accompanying drawings:

example 1

Referring to fig. 1, an embodiment 1 of a negative pressure suction filtration combined column installation device according to the present utility model includes a frame plate 1, wherein a plurality of placement holes 2 are provided on the frame plate 1, a protection tube 3 communicated with the placement holes 2 is fixedly provided on the frame plate 1, and a through hole 4 is provided at the bottom of the protection tube 3; a detachable binding post 5 is arranged in the protective tube 3, and the top of the binding post 5 is opened, so that reagents can be conveniently added into the binding post 5; the bottom of the combination column 5 is provided with an outlet 6, and the outlet 6 passes through the through hole 4 to be communicated with the outside; an adsorption film 7 is arranged above the discharge port 6 in the binding column 5.

The frame plate 1 is used for erecting the combination column 5, and the whole device is supported on the negative pressure suction filtration box 17 by the frame plate 1. The combination post 5 is placed into the protection pipe through the placement hole 2, and the protection pipe is fixed on the frame plate 1, so that the combination post 5 in the protection pipe can be protected from being impacted by external force, and the combination post 5 is prevented from being inclined and damaged due to the impact of the external force. Meanwhile, a plurality of binding columns 5 can be placed on one frame plate 1, so that the operations of suction filtration and purification of the binding columns 5 are achieved at one time; the through port 4 is used for enabling the exhaust port 6 to be communicated with the outside; the discharge port 6 is used for discharging the waste liquid in the binding column 5; the adsorption film 7 serves to adsorb a biological sample required by an operator while allowing impurities to pass through itself with the washing liquid. Specifically, the frame plate 1 is rectangular, the frame plate 1 is used to be placed on an opening at the top of the suction box 17, and the suction box 17 is used to communicate with an external negative pressure suction device (such as a vacuum pump). The placement holes 2 at the top of the frame plate 1 are two rows along the length direction of the frame plate 1, and the number of placement holes 2 in each row is six, so that the negative pressure suction filtration combined column mounting device in the embodiment can perform suction filtration operation on twelve combined columns 5 at one time. The protective tube 3 is vertically disposed on the bottom surface of the shelf plate 1 by ultrasonic welding, and the protective tube 3 is coaxially disposed with the placement hole 2.

In this embodiment, the bottom of the combination column 5 is provided with a column mouth 8, the exhaust port 6 is arranged at the bottom of the column mouth 8, the column mouth 8 is arranged through the passing port 4, and the outer diameter of the column mouth 8 is equal to the inner diameter of the passing port 4.

Specifically, the pillar mouth 8 is cylindrical, and the pillar mouth 8 is coaxially provided with the coupling pillar 5. The discharge opening 6 is arranged coaxially with the pillar mouth 8. In use, waste liquid flows through the nozzle 8 and eventually exits the binding column 5 through the discharge port 6. The internal diameter through mouth 4 is equal with the external diameter of post mouth 8 for when spliced pole 5 inserts protection tube 3, the outer wall of post mouth 8 and the inner wall through mouth 4 butt just, can play the effect of location, spacing to post mouth 8 through mouth 4, prevent that spliced pole 5 from rocking in protection tube 3, also can reduce the clearance between spliced pole 5 and the protection tube 3 simultaneously, when avoiding carrying out the negative pressure suction filtration, make negative pressure suction filtration effect decline because of the clearance between spliced pole 5 and the protection tube 3.

In this embodiment, a sealant layer 9 is disposed on the upper surface of the frame plate 1, and the bonding post 5 is movably inserted into the protection tube 3 through the sealant layer 9 and the placement hole 2; the top of the combination column 5 is provided with a flange 10, the outer diameter of the flange 10 is larger than the inner diameter of the placement hole 2, and the bottom of the flange 10 is abutted with the sealant layer 9.

The upper surface of the frame plate 1 is uniformly adhered with a layer of sealant layer 9, and the sealant layer 9 can be made of silica gel. When the binding post 5 is put into the protective tube 3 from the placement hole 2, the flange 10 is put on the top of the frame plate 1 by abutting against the sealant layer 9, so that the whole binding post 5 is prevented from falling into the protective tube 3, and the binding post 5 is prevented from being taken out of the protective tube 3 inconveniently; through setting up sealant layer 9 on frame plate 1, and the top of combining post 5 is equipped with flange 10, when combining post 5 was placed in protection tube 3, flange 10's external diameter owing to be greater than the external diameter of placing hole 2, flange 10 will with the sealant layer 9 butt that is located frame plate 1 top to form seal structure, prevent when carrying out negative pressure suction filtration, outside air gets into frame plate 1, the inside of suction filtration box 17 through the clearance between combining post 5 and the protection tube, makes frame plate 1, unable sealed structure of formation between the suction filtration box 17, influences the suction filtration effect.

In this embodiment, the locking mechanism 11 capable of locking the coupling post 5 in the protection tube 3 is disposed on the coupling post 5 and the protection tube 3. By arranging the locking mechanism 11, the binding post 5 can be fixed in the protection tube 3 through the locking mechanism 11, so that the stability of the binding post 5 in the suction filtration operation is ensured, and the binding post 5 can be removed from the protection tube 3 by operating the locking mechanism 11 to unlock, so that the detachable effect is achieved; the binding post 5 can be detached and arranged, so that the binding post is convenient to replace, and the number of the binding posts 5 can be increased or decreased according to actual conditions.

In this embodiment, the locking mechanism 11 includes a plurality of sliding blocks 111 and a plurality of limiting bars 112; the limiting strips 112 are obliquely arranged on the inner wall of the protection tube 3, and the sliding blocks 111 are arranged on the outer wall of the combination column 5; the limit bar 112 includes a start end 1121 and a stop end 1122, wherein the start end 1121 is located above the stop end 1122; the sliding block 111 is located below the initial end 1121 and above the final end 1122, and the top of the sliding block 111 is in sliding contact with the bottom of the limit bar 112; the flange 10 gradually abuts against the sealant layer 9 during the movement of the slider 111 along the start end 1121 to the end 1122 of the stop bar 112.

Specifically, two limiting strips 112 are disposed on the inner wall of each protection tube 3, the two limiting strips 112 in each protection tube 3 are disposed with the axis of the protection tube 3 as axisymmetric, two square sliding blocks 111 are disposed on the outer wall of the combining column 5 with the axis of the combining column 5 as axisymmetric, and the sliding blocks 111 correspond to the limiting strips 112 in position. During the use, insert the protection pipe 3 with the binding post 5, take notice at this moment and make slider 111 be located between two spacing 112, be the unblock state at this moment, then rotate binding post 5 for slider 111 removes to the starting end 1121 below of stopper, then continue to rotate binding post 5, make slider 111 remove along spacing 112, because the block of flange 10, slider 111 can't continue down to remove, this just makes slider 111 can with spacing 112 between gradual tight, until can't continue to remove, also can't continue to rotate binding post 5, the binding post 5 is the lock state at this moment.

In this embodiment, a plurality of levers 12 are provided on the top of the coupling post 5. Specifically, the shift levers 12 are located at the top of the flange 10, the number of shift levers 12 on each coupling post 5 is two, the two shift levers 12 are symmetrically arranged on the flange 10 by taking the axis of the coupling post 5 as a symmetry axis, and the shift levers 12, the flange 10 and the coupling post 5 are integrally formed. An operator can dial the combination column 5 through the dial rod 12, so that the operator can conveniently control the combination column 5 to enter a locking state or an unlocking state.

In this embodiment, an apron 13 is disposed on the peripheral side of the frame plate 1, and the apron 13 extends downward of the frame plate 1; the bottom of the apron 13 is provided with a sealing strip 14.

Specifically, an apron 13 is provided on the periphery of the bottom surface of the chassis 1, and the apron 13 extends vertically downward of the chassis 1. The apron 13 is used for abutting with the top of the suction filtration box 17, so that the frame plate 1 is in an overhead state, and a certain gap can be formed between the combining column 5 and the bottom of the suction filtration box 17 no matter what specification the suction filtration box 17 is. The bottom of the apron 13 is adhered with a sealing strip 14 made of silica gel, and the sealing strip 14 is used for reducing a gap between the apron 13 and the suction filtration box 17 and increasing air tightness between the apron 13 and the suction filtration box 17.

In this embodiment, adjacent protection pipes 3 are connected by a reinforcing rib 15. Specifically, square reinforcing ribs 15 are fixedly arranged between adjacent safekeeping and between the protective tube 3 and the apron 13, the reinforcing ribs 15 can increase the stability of the protective tube 3, and the structural strength of the whole pressure suction filtration combined column 5 device can also be improved.

Example 2

Referring to fig. 2, in order to provide an embodiment 2 of a negative pressure suction filtration combined column installation device according to the present utility model, the difference between the present embodiment and the embodiment 1 is that the locking mechanism 11 includes a first thread 113 and a second thread 114, the first thread 113 is disposed on the inner wall of the protection tube 3, and the second thread 114 is disposed on the outer wall of the combined column 5; the coupling post 5 is inserted into the protection tube 3 by the engagement of the first screw 113 and the second screw 114.

Specifically, the binding post 5 is inserted into the protection tube 3, then the binding post 5 is rotated, so that the binding post 5 and the protection tube 3 are connected through the threaded fit of the first threads 113 and the second threads 114, the binding post 5 also enters a locking state at this time, and is connected through the threads, so that the binding post 5 is convenient to install and take out, and the tightness between the binding post 5 and the protection tube 3 can be increased through threaded connection.

Example 3

Referring to fig. 2, in order to provide an embodiment 3 of a negative pressure suction filtration combined column mounting device according to the present utility model, the difference between the present embodiment and embodiment 1 is that the device further includes a sealing plug 16, and the sealing plug 16 is detachably disposed in the placement hole 2.

Specifically, the material of sealing plug 16 is silica gel, and the external diameter of sealing plug 16 is greater than the internal diameter of placing hole 2, and if the required spliced pole 5 quantity of once suction filtration is less than the quantity of placing hole 2, then can pack into idle placing hole 2 with sealing plug 16, because sealing plug 16 is soft silica gel, can get into placing hole 2 through deformation to with the pore wall of placing hole 2 supports tightly, reach sealed effect.

It is to be understood that the above examples of the present utility model are provided by way of illustration only and not by way of limitation of the embodiments of the present utility model. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the utility model are desired to be protected by the following claims.

Claims

1. The negative pressure suction filtration combined column mounting device is characterized by comprising a frame plate (1), wherein a plurality of placing holes (2) are formed in the frame plate (1), a protection tube (3) communicated with the placing holes (2) is fixedly arranged on the frame plate (1), and a through hole (4) is formed in the bottom of the protection tube (3); a detachable combination column (5) is arranged in the protection tube (3), the top of the combination column (5) is opened, an exhaust port (6) is formed in the bottom of the combination column (5), and the exhaust port (6) passes through the through port (4) to be communicated with the outside; an adsorption film (7) is arranged above the discharge port (6) in the binding column (5).

2. The negative pressure suction filtration combined column installation device according to claim 1, wherein a column mouth (8) is arranged at the bottom of the combined column (5), the row mouth (6) is arranged at the bottom of the column mouth (8), the column mouth (8) is penetrated through the through hole (4), and the outer diameter of the column mouth (8) is equal to the inner diameter of the through hole (4).

3. The negative pressure suction filtration combined column installation device according to claim 1, wherein the upper surface of the frame plate (1) is provided with a sealant layer (9), and the combined column (5) is movably inserted into the protection tube (3) through the sealant layer (9) and the placement hole (2); the top of the binding post (5) is provided with a flange (10), the outer diameter of the flange (10) is larger than the inner diameter of the placement hole (2), and the bottom of the flange (10) is abutted to the sealant layer (9).

4. The negative pressure suction filtration binding column installation device according to claim 3, wherein a locking mechanism (11) capable of locking the binding column (5) in the protection tube (3) is arranged on the binding column (5) and the protection tube (3).

5. The negative pressure suction filtration combined column mounting device according to claim 4, wherein the locking mechanism (11) comprises a plurality of sliding blocks (111) and a plurality of limit strips (112); the limiting strips (112) are obliquely arranged on the inner wall of the protection tube (3), and the sliding blocks (111) are arranged on the outer wall of the binding column (5); the limit strip (112) comprises a starting end (1121) and a finishing end (1122), and the starting end (1121) is positioned above the finishing end (1122); the sliding block (111) is positioned below the starting end (1121) and above the finishing end (1122), and the top of the sliding block (111) is in sliding contact with the bottom of the limit strip (112).

6. The negative pressure suction filtration binding column installation device according to claim 4, wherein the locking mechanism (11) comprises a first thread (113) and a second thread (114), the first thread (113) is arranged on the inner wall of the protection tube (3), and the second thread (114) is arranged on the outer wall of the binding column (5); the connecting column (5) is inserted into the protection tube (3) through the cooperation of the first screw thread (113) and the second screw thread (114).

7. The negative pressure suction filtration binding column mounting device according to claim 5 or 6, characterized in that the top of the binding column (5) is provided with a plurality of deflector rods (12).

8. The negative pressure suction filtration binding column mounting device according to claim 1, characterized in that adjacent protection pipes (3) are connected by a reinforcing rib (15).

9. The negative pressure suction filtration combined column mounting device according to claim 1, wherein an apron (13) is arranged on the periphery of the frame plate (1), and the apron (13) extends towards the lower part of the frame plate (1); the bottom of the apron (13) is provided with a sealing strip (14).

10. The negative pressure suction filtration binding column mounting device according to claim 1, further comprising a sealing plug (16), the sealing plug (16) being detachably arranged in the placement hole (2).